Micro-sampling device for liquid chromatography

Abstract

The utility model relates to the technical field of detection devices, in particular to a micro-sampling device for liquid chromatography, which comprises a frame body, a mixing bin is embedded in the inner side of the frame body, the frame body comprises a bottom frame and an operating table, a limiting frame is fixedly mounted on one side of the upper end face of the operating table, and the limiting frame is fixedly connected with the operating table. A liquid tank is movably inserted into the limiting frame, and an air extracting pump and an air injecting pump are fixedly installed on the other side of the upper end face of the operation table. The mixing bin comprises a top bin, holes are formed in the front end and the rear end of one side of the top bin respectively, communicating pipes are embedded in the holes, a hole is formed in the other side of the top bin and communicated with a three-way pipe, and the two ends of the bottom of the three-way pipe are communicated with an air extracting pump and an air injection pump respectively. The material mixing device has the advantages that the material mixing speed is increased, so that the sample injection efficiency is improved, and the problem that the sample injection efficiency is difficult to improve due to low material mixing efficiency of a device in the prior art is solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection device technical field, concretely for a kind of for liquid chromatography trace sample introduction device. BACKGROUND

[0002] Liquid chromatography-mass spectrometry is a highly efficient analytical method, it uses liquid as mobile phase, and by high-pressure pumping system, the mobile phase is injected into the chromatographic column with stationary phase. In the chromatographic column, different polarity components will be separated according to their interaction with the stationary phase. After separation, these components will be guided to the detector for detection. This technology is widely used in chemistry, medicine, industry, agriculture, commercial inspection and forensic medicine, etc. It is an important tool for separating and analyzing samples.

[0003] After a large number of searches, a kind of automatic sampling device for liquid chromatography-mass spectrometer is disclosed in CN218350209U, which can be placed in the liquid placing groove by placing two bottles containing detection liquid in the liquid tank, the liquid in the liquid tank is placed in the measuring cup by the No. 1 liquid pump, the user can observe the capacity of the extracted liquid in the measuring cup, after the extraction is completed, the No. 2 liquid pump extracts the liquid in the measuring cup into the stirring tank for stirring, the motor drives the eccentric gear to eccentric rotate in the main gear through the stirring shaft.

[0004] However, in the prior art, when the solvent and the buffer solution are mixed, the traditional process is used for mixing, and such mixing has a long mixing time, which makes it difficult to improve the working efficiency of the sampling device. Therefore, a kind of for liquid chromatography trace sample introduction device is needed to solve the above problems. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a kind of for liquid chromatography trace sample introduction device, with the advantages of improving mixing speed, and then improving sampling efficiency, solve the problem that the sampling efficiency is difficult to improve due to the low mixing efficiency of the device in the prior art.

[0006] To achieve the above object, the utility model provides the following technical scheme: a kind of for liquid chromatography trace sample introduction device, including frame body, the frame body inside embedded installation has mixing bin, the frame body includes chassis and operation table, the operation table upper end face one side is fixedly installed with limit stop, the limit stop is movably inserted with liquid tank, the operation table upper end face other side is fixedly installed with suction pump and gas injection pump;

[0007] The mixing bin includes top bin, the front and back ends of one side of the top bin are respectively holed and embeddedly installed with communication pipe, the other side of the top bin is holed and connectedly installed with three-way pipe, the bottom of the three-way pipe two ends is respectively communicated with suction pump and gas injection pump and is installed.

[0008] Preferably, the mixing bin also includes a bottom bin, the top of which is fixedly connected to the bottom of the top bin by mounting bolts, and the bottom of the bottom bin is designed in a conical structure and is provided with a discharge valve through a short pipe. The design of the mixing bin also includes a bottom bin, which is fixedly connected to the bottom of the top bin by mounting bolts. This design ensures the stability and durability of the structure. The bottom of the bottom bin is designed in a conical structure and is provided with a discharge valve through a short pipe. This design helps to achieve smoother flow during discharge and reduces residue.

[0009] Preferably, the discharge valve is designed in a spring safety valve structure, the discharge valve is designed in a normally closed safety valve structure, and the spring in the discharge valve is located at the bottom of the inner side of the discharge valve. The design of the discharge valve adopts a spring safety valve structure, which makes the discharge valve remain closed in normal state and only opens under high pressure when discharge is needed. The spring in the discharge valve is located at the bottom of the inner side, which helps to keep the valve closed in a low pressure environment, thereby improving the sealing and safety.

[0010] Preferably, one end of each of the two communication pipes inside the top bin is provided with a one-way valve, and a plug-in pipe is movably inserted into each of the two communication pipes outside the top bin. In the design, a one-way valve is installed on each of the two communication pipes inside the top bin, which ensures that the flow phase can only flow in one direction and prevents backflow and gas leakage. A plug-in pipe is movably inserted into each of the two communication pipes outside the top bin, which allows the plug-in pipe to adjust its position as needed to adapt to different liquid tanks.

[0011] Preferably, the end of the plug-in pipe away from the communication pipe is movably inserted into the inside bottom of the liquid tank, and the plug-in pipe is designed in soft rubber material. In the design, the end of the plug-in pipe away from the communication pipe is movably inserted into the inside bottom of the liquid tank, which helps to more effectively absorb the liquid. The plug-in pipe is designed in soft rubber material, which not only provides good sealing, but also reduces damage to the inner wall of the liquid tank.

[0012] Preferably, a drive motor is fixedly installed on the top of the top bin, a stirring frame is drivingly installed at the bottom end of the drive motor, and the stirring frame penetrates through the top bin and extends into the inside bottom of the bottom bin. In the design, a drive motor is fixedly installed on the top of the top bin, and a stirring frame is drivingly installed at the bottom end of the drive motor. The design of the stirring frame allows it to penetrate through the top bin and extend into the inside bottom of the bottom bin, which ensures the uniformity and efficiency of the mixing process.

[0013] Compared with the prior art, the utility model has the advantages of the following:

[0014] The mixing bin is embeddedly installed on the inner side of the frame body, the design makes the mixing process more concentrated and compact, reduces the moving distance of materials and samples in the mixing process, thereby improving the efficiency, the front and rear ends of the top bin are respectively provided with the communication pipe and the three-way pipe, and the other side is provided with the three-way pipe. This design allows simultaneous air extraction and air injection operation, so that the mobile phase and the stationary phase can be quickly mixed, the two ends of the bottom of the three-way pipe are directly communicated with the air extraction pump and the air injection pump, which can quickly generate negative pressure and positive pressure in the mixing bin, promote the suction and mixing of the mobile phase, the limit frame is fixedly installed on one side of the upper end face of the operation table, and the liquid tank is movably inserted in the limit frame. This design ensures the stable placement and quick replacement of the liquid tank, helps to improve the sample injection efficiency, and the discharge valve adopts a quick response design to quickly discharge the mixed liquid after the mixing is completed, reduce the waiting time, through the design, the liquid chromatography micro sample injection device can reduce the mixing time, improve the uniformity of the mixing, thereby improving the sample injection efficiency. The improvement of the efficiency is particularly important for application scenarios that require rapid analysis of a large number of samples, such as high-throughput screening and rapid diagnostic testing. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a front view structural schematic diagram of the utility model;

[0016] Figure 2 It is a frame body structure schematic diagram of the utility model;

[0017] Figure 3 It is a top bin connecting structure schematic diagram of the utility model;

[0018] Figure 4 It is a bottom bin connecting structure schematic diagram of the utility model.

[0019] In the drawing: 1, frame body; 11, bottom frame; 12, operation table; 121, limit frame; 122, air extraction pump; 123, air injection pump; 2, liquid tank; 3, mixing bin; 31, top bin; 311, three-way pipe; 3111, one-way valve; 312, communication pipe; 3121, plug-in pipe; 32, driving motor; 321, stirring frame; 33, bottom bin; 331, discharge valve. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0021] Embodiment one

[0022] As Figure 1 , Figure 2 , Figure 3 and Figure 4 indicated, an embodiment provided by the utility model: a kind of for liquid chromatogram trace sample introduction device, including frame body 1, frame body 1 inside is embedded and is installed with mixing bin 3, frame body 1 includes chassis 11 and operation table 12, operation table 12 upper end face one side is fixedly installed with limit frame 121, limit frame 121 inside is movably inserted with liquid tank 2, operation table 12 upper end face other side is fixedly installed with suction pump 122 and gas injection pump 123;

[0023] Mixing bin 3 includes top bin 31, the front and rear ends of one side of top bin 31 are respectively perforated and embeddedly installed with communicating pipe 312, the other side of top bin 31 is perforated and connectedly installed with tee pipe 311, and the two ends of bottom of tee pipe 311 are respectively connectedly installed with suction pump 122 and gas injection pump 123.

[0024] Specifically, mixing bin 3 is embeddedly installed inside frame body 1, this design makes mixing process more concentrated and compact, reduces the moving distance of material and sample during mixing process, to improve efficiency, the front and rear ends of one side of top bin 31 are respectively perforated and embeddedly installed with communicating pipe 312, and the other side is perforated and connectedly installed with tee pipe 311. This design allows simultaneous suction and gas injection operation, so that mobile phase and stationary phase can be quickly mixed, and the two ends of bottom of tee pipe 311 are directly connectedly installed with suction pump 122 and gas injection pump 123, so that negative pressure and positive pressure can be quickly generated in mixing bin 3, promoting the inhalation and mixing of mobile phase, and limit frame 121 is fixedly installed on one side of upper end face of operation table 12, and liquid tank 2 is movably inserted in limit frame 121. This design ensures the stable placement and quick replacement of liquid tank 2, helps to improve the efficiency of sample introduction, and the discharge valve 331 adopts a quick response design to quickly discharge the mixed liquid after mixing is completed, reducing the waiting time. Through these designs, the liquid chromatogram trace sample introduction device can reduce the mixing time, improve the uniformity of mixing, and thus improve the sample introduction efficiency. This efficiency improvement is particularly important for application scenarios that require rapid analysis of a large number of samples, such as high-throughput screening and rapid diagnostic testing.

[0025] Embodiment two

[0026] To improve the stability of mixing bin when in use, and can be quickly responded when discharging, as Figure 1 , Figure 3 and Figure 4As shown, the mixing bin 3 also includes a bottom bin 33, which is fixedly connected to the bottom of the top bin 31 through mounting bolts. The bottom of the bottom bin 33 is designed in a conical structure and is provided with a discharge valve 331 through a short tube. The design of the mixing bin 3 also includes a bottom bin 33, which is fixedly connected to the bottom of the top bin 31 through mounting bolts. This design ensures the stability and durability of the structure. The bottom of the bottom bin 33 is designed in a conical structure and is provided with a discharge valve 331 through a short tube. This design helps to achieve smoother flow during discharge and reduces residue.

[0027] Further, the discharge valve 331 adopts a spring safety valve structure design. The discharge valve 331 adopts a normally closed safety valve structure design, and the spring in the discharge valve 331 is located at the bottom of the inside of the discharge valve 331. In the design, the discharge valve 331 adopts a spring safety valve structure. This design makes the discharge valve 331 remain closed in normal state, and only opens under high pressure when it needs to discharge. The spring in the discharge valve 331 is located at the bottom of the inside, which helps to keep the valve closed in low pressure environment, thereby improving the sealing and safety.

[0028] Further, one end of each of the two communication pipes 312 inside the top bin 31 is provided with a one-way valve 3111, and each of the two communication pipes 312 outside the top bin 31 is movably inserted with a plug-in pipe 3121. In the design, the two communication pipes 312 inside the top bin 31 are respectively provided with one-way valves 3111, which ensures that the flow phase can only flow in one direction, preventing backflow and gas leakage. The two communication pipes 312 outside the top bin 31 are movably inserted with plug-in pipes 3121, which allows the plug-in pipes 3121 to adjust the position as needed to adapt to different liquid tanks 2.

[0029] Example Three

[0030] In order to improve the working efficiency of the mixing bin when mixing, as shown in Figure 3 and Figure 4 As shown, one end of the plug-in pipe 3121 away from the communication pipe 312 is movably inserted into the inside bottom of the liquid tank 2, and the plug-in pipe 3121 is designed with soft rubber material. In the design, one end of the plug-in pipe 3121 away from the communication pipe 312 is movably inserted into the inside bottom of the liquid tank 2, which helps to more effectively absorb the liquid. The plug-in pipe 3121 is made of soft rubber material, which not only provides good sealing, but also reduces damage to the inner wall of the liquid tank 2.

[0031] Further, the top of the top bin 31 is fixedly provided with a driving motor 32, and the bottom end of the driving motor 32 is drivingly provided with a stirring frame 321, and the stirring frame 321 penetrates through the top bin 31 and extends to the bottom inside of the bottom bin 33. In the design, the top of the top bin 31 is fixedly provided with the driving motor 32, and the bottom end of the driving motor 32 is drivingly provided with the stirring frame 321. The design of the stirring frame 321 enables the stirring frame 321 to penetrate through the top bin 31 and extend to the bottom inside of the bottom bin 33, and the design ensures the uniformity and efficiency of the mixing process.

[0032] In use, the liquid tank 2 is placed in the limiting frame 121 on the upper end face of the operation table 12, so that the liquid tank 2 can be freely inserted and fixed, the air pump 122 and the gas injection pump 123 are fixedly installed on the other side of the upper end face of the operation table 12, and whether the connection is firm is checked, the air pump 122 is started, the negative pressure is generated in the mixing bin 3 through the air pump 122, and the mobile phase in the liquid tank 2 is sucked through the communication pipe 312 on one side of the top bin 31, the mobile phase is sucked into the mixing bin 3 through the communication pipe 312, the driving motor 32 is started, and the driving motor 32 stirs between the top bin 31 and the bottom bin 33 through the stirring frame 321, so as to ensure that the mobile phase and the fixed phase are fully mixed, the stirring action of the stirring frame 321 can improve the mixing efficiency, the mixing of the mobile phase and the fixed phase is more uniform, the gas injection pump 123 is started, the high-pressure gas enters the mixing bin 3 through the three-way pipe 311, and the gas is prevented from leaking out through the one-way valve 3111, the mixing of the mobile phase and the fixed phase is promoted by increasing the pressure in the mixing bin 3, and after the mixing is completed, the discharge is prepared. At this time, the discharge valve 331 is in a normally closed state to prevent liquid leakage during the mixing process, the discharge valve 331 is automatically opened by continuing to inject gas, the mixed liquid in the mixing bin 3 flows into the discharge valve 331 through the conical structure and the short pipe of the bottom bin 33, and then is discharged to the outlet device, and is prepared for chromatographic analysis.

[0033] It is apparent for a person skilled in the art that the present application is not limited to the details of the above-described exemplary embodiments, but can be implemented in other concrete forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be considered in all aspects as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and range of equivalents of the essential elements of the claims are intended to be embraced in the present application. Any reference signs in the claims should not be considered as limiting the claims involved.

Claims

1. A liquid chromatography micro-injection device, comprising a frame (1), a mixing bin (3) is embedded and installed inside the frame (1), characterized in that: the frame (1) comprises a chassis (11) and an operation table (12), a limiting frame (121) is fixedly installed on one side of the upper end surface of the operation table (12), a liquid tank (2) is movably inserted into the limiting frame (121), an air extraction pump (122) and an air injection pump (123) are fixedly installed on the other side of the upper end surface of the operation table (12); the mixing bin (3) comprises a top bin (31), two communication pipes (312) are respectively embedded and installed on the front and rear ends of one side of the top bin (31), a three-way pipe (311) is communicated and installed on the other side of the top bin (31), and the bottom ends of the three-way pipe (311) are communicated and installed with the air extraction pump (122) and the air injection pump (123).

2. A device for microsyringing liquid chromatography as defined in claim 1, wherein, The mixing bin (3) further comprises a bottom bin (33), the top of the bottom bin (33) is fixedly connected with the bottom of the top bin (31) through mounting bolts, the bottom of the bottom bin (33) is designed in a conical structure and is communicated and installed with a discharge valve (331) through a short pipe.

3. A device for microsyringing liquid chromatography as defined in claim 2, wherein, The discharge valve (331) is designed in a spring type safety valve structure, the discharge valve (331) is designed in a normally closed safety valve structure, and the spring in the discharge valve (331) is arranged at the bottom inside of the discharge valve (331).

4. The device of claim 1 wherein, One end of each of the two communication pipes (312) inside the top bin (31) is communicated and installed with a one-way valve (3111), and the two communication pipes (312) outside the top bin (31) are movably inserted with plug-in pipes (3121).

5. A device for microsyringing liquid chromatography as defined in claim 4, wherein, One end of the plug-in pipe (3121) away from the communication pipe (312) is movably inserted into the bottom inside of the liquid tank (2), and the plug-in pipe (3121) is designed in soft rubber material.

6. The device of claim 1 wherein, A driving motor (32) is fixedly installed on the top of the top bin (31), a stirring frame (321) is drivingly installed at the bottom end of the driving motor (32), and the stirring frame (321) penetrates through the top bin (31) and extends into the bottom inside of the bottom bin (33).

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