Imprinting method protein detection device

Abstract

The utility model discloses an imprinting method protein detection device, which belongs to the technical field of biochips and diagnostic reagents, and comprises a reaction kit and a waste liquid recovery device, the reaction kit comprises a bottom plate and a box body, a pipeline is arranged on the side wall of the box body, one end of the pipeline is communicated with the inside of the box body, and the other end of the pipeline is connected with a waste liquid pipe; the waste liquid recovery device comprises a support, a rotating disc is arranged in the center of the support, a through hole is formed in the rotating disc, the support and the rotating disc are connected through a plurality of connecting bridges, a plurality of connecting holes are formed in the support, the positions of the connecting holes correspond to the positions of the connecting bridges, and the waste liquid pipe is connected with the connecting holes. The imprinting method protein detection device disclosed by the utility model can be used for controlling the reaction time and realizing rapid and automatic recovery of waste liquid.

Description

Technical Field

[0001] This utility model relates to the field of biochip and diagnostic reagent technology, and in particular to a protein detection device using the imprinting method. Background Technology

[0002] Western blotting is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics. Its basic principle is to stain cell or tissue samples treated with gel electrophoresis with specific antibodies. By analyzing the location and depth of the staining, information about the expression of specific proteins in the analyzed cells or tissues can be obtained.

[0003] Protein blotting requires antibody cultivation of the protein-loaded membrane. For example, Chinese patent CN 207292804 U discloses a protein blotting kit that saves antibody usage. Specifically, during antibody cultivation, the antibody solution is appropriately diluted, and the diluted antibody solution is dropped onto a square carrier plate. A nitrocellulose membrane is taken out from the storage box and placed over the antibody solution, ensuring that the nitrocellulose membrane is evenly distributed to avoid the formation of air bubbles. The nitrocellulose membrane reduces the evaporation of the antibody solution. The evaporation of the antibody solution is further reduced by the snap-fit ​​mechanism of the box lid and body and by temperature regulation, thus saving antibody solution and facilitating the recovery of excess antibody solution.

[0004] The disadvantages of the above-mentioned patent are that air bubbles need to be avoided when covering with nitrocellulose membrane, which requires a high level of experience from the operator and is not suitable for beginners. Secondly, when recovering the antibody solution, the lid of the reagent kit needs to be opened, the nitrocellulose membrane needs to be removed, and then the antibody solution needs to be poured out, which is cumbersome and prone to problems. Utility Model Content

[0005] The technical problem to be solved by this invention is to design a novel imprinting protein detection device that is not only easy to operate, but also allows for control of reaction time and enables rapid and automatic recovery of waste liquid.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: a blot protein detection device, comprising a reaction kit and a waste liquid recovery device; the reaction kit comprises a base plate and a box body, the box body being snapped onto the base plate, a pipe being provided on the side wall of the box body, one end of the pipe communicating with the interior of the box body, the other end being connected to a waste liquid pipe, and the other end of the waste liquid pipe communicating with the outside; the waste liquid recovery device comprises a support, the interior of the support being a hollow cavity, a turntable being provided at the center of the support, the turntable having through holes, the support and the turntable being connected by a plurality of connecting bridges, the connecting bridges being used to fix the reaction kit; a plurality of connecting holes being provided on the support, the positions of the connecting holes corresponding to the connecting bridges, and the waste liquid pipe being connected to the connecting holes.

[0007] Furthermore, the upper surface of the box is provided with a sample dispensing hole and an observation hole, and the interior of the box is provided with a sample dispensing channel, an observation channel and a recovery chamber. The sample dispensing hole is connected to the sample dispensing channel, and the observation hole is connected to the observation channel.

[0008] Furthermore, the upper surface of the base plate is provided with a sample addition area, a reaction area and a recovery area in sequence. The sample addition area is connected to the sample addition channel, the reaction area is connected to the observation channel, and the recovery area is connected to the recovery chamber.

[0009] Furthermore, the bottom shapes of the sample dispensing channel, observation channel, and recovery chamber are respectively adapted to the edge shapes of the sample dispensing area, reaction area, and recovery area.

[0010] Furthermore, two slots are provided opposite each other on the two sides of the reaction zone, which are higher than the sample addition zone and the recovery zone.

[0011] Furthermore, a protrusion is provided at the end opposite to the connection between the recovery zone and the reaction zone, and the protrusion extends into the bottom of the pipe.

[0012] Furthermore, a sealing ring is provided at the connection between the base plate and the box body.

[0013] Furthermore, both the support and the turntable are circular.

[0014] Beneficial effects: This application sets a pipe on the side wall of the reaction kit to output the waste liquid in the reaction chamber to the waste liquid pipe. At the same time, the reaction kit is fixed on the waste liquid recovery device. The rotation speed of the waste liquid recovery device can be controlled by the motor. This not only controls the reaction time, but also throws the waste liquid in the reaction kit into the cavity of the waste liquid recovery device support through the waste liquid pipe, thus realizing the automatic recovery of waste liquid. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the reaction kit of this utility model.

[0016] Figure 2 This is a schematic diagram of the cross-section of the reaction kit of this utility model.

[0017] Figure 3 This is a schematic diagram of the waste liquid recovery device of this utility model.

[0018] The components are: 1-Reaction kit, 11-Base plate, 111-Sample application area, 112-Reaction area, 113-Recovery area, 114-Protrusion, 12-Box body, 121-Sample application hole, 122-Observation hole, 123-Sample application channel, 124-Observation channel, 125-Recovery chamber, 13-Reaction chamber, 14-Pipeline, 15-Waste liquid pipe, 2-Waste liquid recovery device, 21-Support, 211-Connection hole, 22-Rotator, 221-Through hole, 23-Connecting bridge. Detailed Implementation

[0019] To enhance understanding of this utility model, it will be described in detail below with reference to the embodiments and accompanying drawings. These embodiments are only used to explain this utility model and do not limit the scope of protection of this utility model.

[0020] like Figure 1 and Figure 2 The diagram shows the structure of reaction kit 1 in the blotting protein detection device. Reaction kit 1 specifically includes a base plate 11 and a housing 12. The housing 12 can be fastened to the base plate 11, and a sealing ring can be placed at the fastening point to prevent leakage.

[0021] The upper surface of the box 12 is provided with a sample dispensing hole 121 and an observation hole 122. The interior is provided with a sample dispensing channel 123, an observation channel 124 and a recovery chamber 125. The sample dispensing hole 121 is connected to the sample dispensing channel 123, the observation hole 122 is connected to the observation channel 124, and the recovery chamber 125 has only one end open.

[0022] Corresponding to the housing 12, a sample application area 111, a reaction area 112, and a recovery area 113 are sequentially arranged on the upper surface of the base plate 11. The bottom shapes of the sample application channel 123, the observation channel 124, and the recovery chamber 125 are adapted to the edge shapes of the sample application area 111, the reaction area 112, and the recovery area 113, respectively. After the housing 12 and the base plate 11 are fastened together, the sample application area 111 and the sample application channel 123 are connected, the reaction area 112 and the observation channel 124 are connected, and the recovery area 113 and the recovery chamber 125 are connected, forming their respective relatively independent areas. However, liquid can flow between these areas through liquid flow channels.

[0023] According to a preferred embodiment of the present invention, such as Figure 2As shown, the connection between reaction zone 112 and sample loading zone 111 is designed as a ramp to facilitate liquid flow into reaction zone 111. An outlet is provided at the connection between reaction zone 112 and recovery zone 113 to facilitate liquid flow out into recovery zone 113. The liquid flow method can be varied and is not limited here.

[0024] According to another preferred embodiment of the present invention, two slots are provided opposite to the portions of the reaction zone 112 that are higher than the sample loading zone 111 and the recovery zone 113, and the NC membrane can be inserted into the slots for fixation.

[0025] A pipe 14 is provided on the side wall of the box 12. One end of the pipe 14 is connected to the bottom of the recovery chamber 125, and the other end is connected to the waste liquid pipe 15. The other end of the waste liquid pipe 15 is connected to the outside.

[0026] To facilitate the flow of waste liquid from the recycling area 113 into the pipe 14, a protrusion 114 that serves as a guide can be provided at the point where the recycling area 113 contacts the pipe 14. The protrusion 114 extends into the bottom of the pipe 14, and the bottom opening of the pipe 14 is slightly higher than the protrusion 114 to facilitate the flow of waste liquid.

[0027] like Figure 3 The waste liquid recovery device 2 shown includes a circular support 21, the interior of which is a hollow cavity. A circular turntable 22 is positioned at the center of the support 21, and the support 21 and the turntable 22 are connected by several connecting bridges 23. The support 21 has several connecting holes 211, the positions of which correspond to the positions of the connecting bridges 23, i.e., one connecting bridge 23 corresponds to one connecting hole 211. The turntable 22 has several through holes 221 for connecting to the motor shaft.

[0028] In use, the reaction kit 1 is fixed on the connecting bridge 23, and the waste liquid tube 15 on the reaction kit 1 is inserted into the connecting hole 211. When the motor is turned on, the waste liquid collection device will be rotated through the turntable 22. The rotation speed is controlled to rotate at a low speed first to facilitate the reaction in the reaction zone. When the reaction is completed, the motor rotates at a high speed, so that the waste liquid in the reaction kit is thrown into the cavity of the support 21 through the waste liquid tube, thus completing the automatic collection of waste liquid.

[0029] The embodiments disclosed herein are preferred embodiments, but are not limited thereto. Those skilled in the art can readily grasp the spirit of this utility model based on the above embodiments and make different extensions and variations. However, as long as they do not depart from the spirit of this utility model, they are all within the protection scope of this utility model.

Claims

1. A blotting protein detection device, characterized in that, Includes a reaction kit (1) and a waste liquid recovery device (2); The reaction kit (1) includes a base plate (11) and a box body (12). The box body (12) is fastened to the base plate (11). A pipe (14) is provided on the side wall of the box body (12). One end of the pipe (14) is connected to the inside of the box body (12), and the other end is connected to the waste liquid pipe (15). The other end of the waste liquid pipe (15) is connected to the outside. The waste liquid recovery device (2) includes a support (21), the interior of which is a hollow cavity. A turntable (22) is set at the center of the support (21), and a through hole (221) is provided on the turntable (22). The support (21) and the turntable (22) are connected by several connecting bridges (23), and the connecting bridges (23) are used to fix the reaction kit (1). The bracket (21) is provided with a plurality of connection holes (211), the position of the connection holes (211) corresponds to the connection bridge (23), and the waste liquid pipe (15) is connected to the connection holes (211).

2. The immunoblotting protein detection device according to claim 1, characterized in that, The upper surface of the box (12) is provided with a sample dispensing hole (121) and an observation hole (122). The interior of the box (12) is provided with a sample dispensing channel (123), an observation channel (124) and a recovery chamber (125). The sample dispensing hole (121) is connected to the sample dispensing channel (123), and the observation hole (122) is connected to the observation channel (124).

3. The imprinting protein detection device according to claim 2, characterized in that, The upper surface of the base plate (11) is provided with a sample addition area (111), a reaction area (112) and a recovery area (113) in sequence. The sample addition area (111) is connected to the sample addition channel (123), the reaction area (112) is connected to the observation channel (124), and the recovery area (113) is connected to the recovery chamber (125).

4. The imprinting protein detection device according to claim 3, characterized in that, The bottom shapes of the sample loading channel (123), observation channel (124) and recovery chamber (125) are adapted to the edge shapes of the sample loading area (111), reaction area (112) and recovery area (113), respectively.

5. The imprinting protein detection device according to claim 4, characterized in that, Two slots are provided opposite each other on the two sides of the reaction zone (112) above the sample loading zone (111) and the recovery zone (113).

6. The imprinting protein detection device according to claim 3, characterized in that, A protrusion (114) is provided at one end opposite to the connection between the recycling zone (113) and the reaction zone (112), and the protrusion (114) extends into the bottom of the pipe (14).

7. The immunoblotting protein detection device according to claim 1, characterized in that, A sealing ring is provided at the connection between the base plate (11) and the box body (12).

8. The immunoblotting protein detection device according to claim 1, characterized in that, Both the support (21) and the turntable (22) are circular.

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