Disposable sterile closed microorganism collecting, filtering and inoculating device
By designing a disposable, sterile, closed microbial collection, filtration, and inoculation device, and utilizing the principle of negative pressure and the pusher plate, the cumbersome and contamination problems of liquid specimen collection and filter membrane inoculation processes are solved, achieving simplified operation and cost reduction.
Patent Information
- Application Number
- CN202422848078.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-21
- Publication Date
- 2025-10-31
- Estimated Expiration
- 2034-11-21
AI Technical Summary
In existing technologies, the process of collecting liquid specimens and inoculating filter membranes is cumbersome, requires multiple sterilizations, is prone to aerosol pollution, and involves high equipment costs, complex operations, and a large workload.
This disposable, sterile, closed microbial collection, filtration, and inoculation device is designed using the principle of negative pressure. It connects to a negative pressure extraction device via a filter cap connector and utilizes a push plate and a pressing rod to achieve liquid filtration and filter membrane inoculation. It avoids contact with foreign objects, is simple to operate, and has good sealing performance.
It enables fully enclosed operation without repeated disinfection, reducing workload, improving work efficiency, lowering equipment costs, avoiding liquid contamination, and simplifying the process.
Smart Images
Figure CN223496466U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of medical device technology, specifically to a disposable sterile closed microbial collection, filtration and inoculation device. Background Technology
[0002] The membrane filtration method refers to the process of collecting liquid specimens under sterile conditions, filtration and concentration through a filter membrane, inoculating the filter membrane onto a petri dish, and then culturing it in an incubator.
[0003] Current technical operation procedure: Liquid specimens are collected in sterile containers in the clinic, filtered in the laboratory using a membrane filtration machine, and then the membrane is moved to a petri dish containing culture medium using an external object (such as tweezers) to complete the inoculation.
[0004] The disadvantages are as follows:
[0005] 1. Containers for collecting liquid specimens need to be sterilized; 2. Filter membrane machines are relatively expensive, requiring pre-sterilization before use, repeated sterilization during use, and cleaning and disinfection after use. Inadequate cleaning and disinfection can easily lead to contamination; 3. Aerosols are easily generated during the process of pouring liquid specimens into the filter membrane machine, and improper operation can contaminate the liquid specimens; 4. Filter membranes are sterilized products that need to be purchased separately. They need to be moved using external objects during and after use, and these external objects also need to be disinfected; 5. The operation process is relatively complicated, increasing the workload of staff.
[0006] Therefore, the purpose of this utility model is to solve the above-mentioned problems and provide a disposable membrane filter sterile collection device. Utility Model Content
[0007] To address the existing defects and problems, this utility model provides a disposable sterile closed microbial collection, filtration and inoculation device.
[0008] The solution adopted by this utility model to solve its technical problem is as follows: using the principle of negative pressure, the existing negative pressure extraction equipment is connected to the filter cap connector to draw out the liquid in the collection bottle, and a pressure difference is formed on the inside and outside of the liquid pusher plate in the bottle, so that the liquid pusher plate pushes the liquid sample through the filter membrane. After the negative pressure extraction is completed, the connecting tube is removed, and the protruding pressing rod is pressed to remove it from the filter membrane by dipping it on the culture dish.
[0009] The specific details of this plan are:
[0010] A disposable sterile closed microbial collection, filtration, and inoculation device includes a collection bottle and a filter cap. The collection bottle is cylindrical with an internally and externally connected vent at its bottom. An openable sealing cap is located outside the vent. A sliding liquid-pushing plate is installed inside the collection bottle, sealing it to the inner wall. The top of the collection bottle is movably and sealingly connected to the bottom of the filter cap. A negative pressure connector is located on the top of the filter cap, and a filter membrane support is fitted inside the filter cap. An upward-facing pressing rod is located at the center of the filter membrane support. The pressing rod extends through the negative pressure connector, with a protrusion length greater than the height from the bottom of the filter membrane support to the opening of the sealing cap. The diameter of the pressing rod is smaller than the inner diameter of the negative pressure connector to avoid affecting liquid flow. A filter membrane covers the bottom of the filter membrane support, and a sealing ring is provided on the outer edge of the filter membrane to ensure the airtightness between the filter cap and the collection bottle, while also ensuring the airtightness between the filter membrane and the outer edge of the filter membrane support. Liquid passes through the central filter hole.
[0011] The collection bottle and filter cap are cylindrical, with an external thread at the top of the collection bottle and an internal thread at the top of the filter cap. The liquid pusher plate is inserted from the top of the collection bottle and slides to the bottom, which facilitates production and installation and maximizes liquid filtration and extraction.
[0012] The liquid pushing plate is equipped with a flexible outer edge tail wing, and the bottom of the liquid pushing plate has a groove structure that matches the concave bottom of the collection bottle. A vent hole is provided in the concave bottom of the collection bottle, and the opening and sealing cap of the vent hole is also located in the concave area, not exceeding the outer edge of the bottom of the bottle, which increases the stability of the sampled liquid.
[0013] The vent opening and sealing cap adopts an easy-tear structure and is integrally formed with the collection bottle, reducing accessories, making operation convenient, and can be opened by puncturing with a hard object or tearing by hand, thus improving efficiency.
[0014] The liquid-pushing plate has an I-shaped double-layer structure, which can further increase stability and sealing.
[0015] The filter membrane support has side holes or slots on its side wall, which communicate with the back of the filter membrane support, so that the filter membrane can fall off into the culture medium vessel. By utilizing the communication between the inside and outside of the culture medium liquid, the filter membrane can be pushed open from the inside of the filter membrane support.
[0016] To reduce leakage and contamination, a backup adsorption layer is installed at the bottom of the collection bottle, which mainly addresses the leakage of liquid from the inner wall during the negative pressure adsorption process.
[0017] The outer edge of the filter membrane support is provided with a sealing rubber ring. This design can increase the sealing of the filtrate and at the same time increase the damping to prevent the filter membrane support from falling out of the filter cover when the pressing rod is operated.
[0018] The filter membrane holder pressing rod is equipped with an anti-detachment protrusion, which can play a limiting role and prevent the filter membrane holder from detaching. The anti-detachment protrusion is located on the side of the rear end of the pressing rod, with a width smaller than the outer diameter of the negative pressure connector and larger than its inner diameter. The shape is dot-shaped or strip-shaped and does not affect the drainage.
[0019] The collection bottle is made of transparent PP material and has a scale on the outer wall for easy observation of the sample collection volume.
[0020] This invention is for single use only, eliminating the need for repeated cleaning and disinfection. It features a fully enclosed filtration process, requiring no external tools to move the filter membrane for inoculation. It is simple to operate, convenient to use, reduces workload, is cost-effective, and significantly improves work efficiency. The sterile, individually packaged membrane is ready to use immediately after opening, requiring no further sterilization. When membrane filtration is needed, simply connect the bottle opening to a negative pressure suction device, activate the negative pressure suction, and the filtration process is complete. Open the filter cap, gently press the filter membrane support, and transfer the filter membrane into the culture dish. Attached Figure Description
[0021] Figure 1 This is a schematic diagram of the combined structure of this utility model.
[0022] Figure 2 This is an exploded structural diagram of the present invention.
[0023] Figure 3 This is a schematic diagram of the exploded cross-section of this utility model.
[0024] Figure 4 This is a schematic diagram of the structure of this utility model viewed from below.
[0025] Figure 5 This is a schematic diagram of the bottom placement of this utility model.
[0026] Figure 6 This is a side view of the filter membrane holder of this utility model.
[0027] Figure 7 This is a bottom view of the filter membrane support of this utility model.
[0028] Figure 8 This is a schematic diagram of the negative pressure connection of this utility model.
[0029] Figure 9 This is a schematic diagram of the filter membrane detachment of this utility model.
[0030] The following labels are used in the diagram: 1 is the filter cap, 2 is the collection bottle, 3 is the liquid pusher plate, 4 is the filter membrane, 5 is the filter membrane support, 51 is the pressing rod, 52 is the sealing rubber ring, 53 is the side hole of the filter membrane support, 54 is the filter hole, 6 is the opening and sealing cap, 7 is the negative pressure connector, 8 is the negative pressure connector end cap, 9 is the negative pressure connecting tube, 10 is the operating finger, and 11 is the culture medium vessel. Detailed Implementation
[0031] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0032] Example 1: A disposable sterile closed microbial collection, filtration, and inoculation device includes a collection bottle 2 and a filter cap 1. The collection bottle is cylindrical, with an internally and externally connected vent at the bottom. An openable sealing cap 6 is located outside the vent. A sliding liquid-pushing plate 3 is installed inside the collection bottle, which is sealed to the inner wall of the collection bottle. The top of the collection bottle is movably and sealed to the bottom of the filter cap. A negative pressure connector 7 is installed on the top of the filter cap. A filter membrane support 5 is fitted inside the filter cap, with an upward-pointing pressing rod 51 at its center. The pressing rod extends from the negative pressure connector 7, with a protrusion length greater than the height from the bottom of the filter membrane support 5 to the opening of the sealing cap 1. The diameter of the pressing rod 51 is smaller than the inner diameter of the negative pressure connector 7, so as not to affect the liquid flow. A filter membrane 4 covers the bottom of the filter membrane support 5, and a sealing ring is provided on the outer edge of the filter membrane 4 to ensure the airtightness of the filter cap 1 and the collection bottle 2, while also ensuring the airtightness of the filter membrane 4 and the outer edge of the filter membrane support 5. Liquid passes through the central filter hole 54.
[0033] The collection bottle 2 and the filter cover 1 are cylindrical. The upper opening of the collection bottle 2 has an external thread, and the filter cover 1 has an internal thread. The liquid pusher 3 is inserted from the upper opening of the collection bottle 2 and slides to the bottom. This is beneficial for production and installation, and can also maximize the liquid filtration and extraction.
[0034] The liquid pushing plate 3 is equipped with a flexible outer edge tail wing, and the bottom of the liquid pushing plate 3 has a groove structure, which matches the concave bottom of the collection bottle 2. A vent hole is provided in the concave bottom of the collection bottle, and the opening and sealing cap 6 of the vent hole is also located in the concave part, not exceeding the outer edge of the bottom of the bottle, which increases the stability of the sampled liquid.
[0035] The vent opening and sealing cap 6 adopts an easy-tear structure and is integrally formed with the collection bottle 2, reducing accessories, making operation convenient, and can be opened by puncturing with a hard object or tearing by hand, thus improving efficiency.
[0036] The filter membrane support 5 has a filter membrane support side hole 53 or a groove on its side wall, which communicates with the back of the filter membrane support, so that the filter membrane 4 can fall off into the culture medium vessel. By utilizing the communication between the inside and outside of the culture medium liquid, the filter membrane can be pushed open from the inside of the filter membrane support.
[0037] Example 2, see Figure 8 The method is largely the same as the embodiment, except that, in order to reduce leakage of the seepage liquid and cause pollution, a spare adsorption layer is set at the bottom of the collection bottle. This mainly solves the problem of liquid seepage from the inner wall during the negative pressure adsorption process of the collection bottle. The adsorption layer can be made of non-woven fabric, absorbent cotton, or other materials.
[0038] Example 3, see Figure 3 It is largely the same as the embodiment, except that the liquid pushing plate has an I-shaped double-layer structure, which can further increase stability and sealing.
[0039] Example 4, see Figure 9The design is largely the same as the embodiment, except that a sealing rubber ring is provided on the outer edge of the filter membrane support 5. This design can increase the sealing of the filtrate and at the same time increase the damping to prevent the filter membrane support 5 from falling out of the filter cover 1 when the pressing rod is operated. The sealing ring can be a flexible outer layer integrated with the filter membrane support, or it can be a rubber ring nested in the outer edge groove. It is not marked in the figure and is a conventional technical means.
[0040] Example 5, see Figure 9 The method is largely the same as the embodiment, except that the filter membrane holder pressing rod 51 is provided with an anti-detachment protrusion, which can play a limiting role and prevent the filter membrane holder 5 from falling out. The anti-detachment protrusion is located on the rear side of the pressing rod 51, and its width is smaller than the outer diameter of the negative pressure connector 7 but larger than its inner diameter. Its shape is dot-shaped or strip-shaped, which does not affect the drainage. In this way, even if the operator is not familiar with it, it will not cause the filter membrane holder to fall off when operated with one hand, thus reducing the difficulty of operation.
[0041] Example 6, see Figure 1 The method is largely the same as the embodiment, except that the collection bottle 2 is made of transparent PP material and has a scale on the outer wall for easy observation of the sample collection volume. The scale is not drawn separately; please refer to [reference needed]. Figure 1 Commonly used scale marking methods.
[0042] In addition to the above embodiments, the disposable sterile closed microbial collection, filtration and inoculation device can also use a snap-fit method, such as a press-lock, in addition to the threaded connection between the collection bottle 2 and the filter cap 1. Considering the sealing and the commonly used operation methods in hospitals, a circular thread is still preferred.
Claims
1. A disposable sterile closed microbial collection, filtration, and inoculation device, comprising a collection bottle and a filter cap, characterized in that: The collection bottle is cylindrical with a vent at the bottom that connects the inside and outside. An openable sealing cap is located outside the vent. Inside the collection bottle is a sliding liquid-pushing plate that is sealed to the inner wall of the bottle. The top of the collection bottle is movably and sealingly connected to the bottom of the filter cap. A negative pressure connector is located on the top of the filter cap, and a filter membrane support is fitted inside the filter cap. An upward-pointing pressing rod is located at the center of the filter membrane support. The pressing rod extends from the negative pressure connector, with a protrusion length greater than the height from the bottom of the filter membrane support to the sealing cap opening. The diameter of the pressing rod is smaller than the inner diameter of the negative pressure connector to avoid obstructing liquid flow. A filter membrane covers the bottom of the filter membrane support, and a sealing ring is located on the outer edge of the filter membrane to ensure a tight seal between the filter cap and the collection bottle, while also ensuring a tight seal between the filter membrane and the outer edge of the filter membrane support. Liquid flows through the middle of the filter membrane.
2. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: The collection bottle and filter cap are cylindrical, with an external thread at the top of the collection bottle and an internal thread at the top of the filter cap. The liquid pusher is inserted into the collection bottle from the top and slides to the bottom.
3. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 2, characterized in that: The liquid pushing plate is equipped with a flexible outer edge tail wing, and the bottom of the liquid pushing plate has a groove structure to match the concave bottom of the collection bottle. A vent hole is provided in the concave bottom of the collection bottle, and the opening and sealing cap of the vent hole is also located in the concave area, not exceeding the outer edge of the bottom of the bottle.
4. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: The vent opening and sealing cap has an easy-tear structure and is integrally formed with the collection bottle.
5. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: The liquid-pushing plate has an I-shaped double-layer structure.
6. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: The filter membrane support has side holes or slots on its side wall, which communicate with the back of the filter membrane support, so as to facilitate the detachment of the filter membrane from the culture medium vessel.
7. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: A spare adsorption layer is provided at the bottom of the collection bottle to reduce leakage of the seepage liquid.
8. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: A sealing rubber ring is provided on the outer edge of the filter membrane holder.
9. The disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: The filter membrane holder pressing rod is equipped with anti-detachment protrusions.
10. A disposable sterile closed microbial collection, filtration, and inoculation device according to claim 1, characterized in that: The collection bottle is made of transparent PP material and has a scale on the outer wall.