EGFR (epidermal growth factor receptor) gene mutation multiple detection kit
By introducing a sample-dispensing mechanism and a septum tray structure into the EGFR gene mutation multiplex detection kit, the problem of uneven sample pouring is solved, ensuring the accuracy of sample pouring and the protection of reagent tubes, thereby improving the accuracy and reliability of detection.
Patent Information
- Application Number
- CN202422887410.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-25
- Publication Date
- 2025-10-31
- Estimated Expiration
- 2034-11-25
AI Technical Summary
Existing EGFR gene mutation multiplex detection kits are not uniform when the sample is poured into each test tube, leading to false negative or false positive results.
A multiplex EGFR gene mutation detection kit was designed, which includes a dropper mechanism. The dropper mechanism delivers the sample dropwise into the reagent tube, ensuring the accuracy of the sample volume. The kit is also separated from other containers by partitions and trays to prevent damage from collisions.
This ensures uniform sample pouring, avoids false negative or false positive results, protects reagent tubes from damage, and improves the accuracy and reliability of the test.
Smart Images
Figure CN223496472U_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of gene detection technology, specifically to an EGFR gene mutation multiplex detection kit. Background Technology
[0002] EGFR gene mutation multiplex assay kits are molecular diagnostic tools used to detect mutations in the epidermal growth factor receptor (EGFR) gene, and are particularly clinically significant in lung cancer and related diseases. This detection method typically employs quantitative real-time PCR (qPCR) technology, which can efficiently and accurately identify specific EGFR mutations. However, existing multiplex assay kits require pouring the sample into each test tube, but the amount poured cannot be accurately controlled during this process, leading to variations in sample concentration in each tube and potentially causing false negative or false positive results due to sample inhomogeneity. Utility Model Content
[0003] To address the shortcomings of existing technologies, this invention provides an EGFR gene mutation multiplex detection kit, which solves the technical problem of uneven sample pouring into each reagent tube.
[0004] To solve the above-mentioned technical problems, the present invention provides the following technical solution: an EGFR gene mutation multiplex detection kit, including a box body and a box cover connected to the box body. A fixing plate is fixedly provided inside the box body. The fixing plate has multiple placement holes one and one placement hole two. A sponge ring is fixedly provided in the placement hole one. A reagent tube containing a fluorescently labeled probe is placed inside the sponge ring. A sample tube is placed in the placement hole two. A sample dispensing mechanism is also slidably provided on the fixing plate to add the sample dropwise into the reagent tube.
[0005] The sample dispensing mechanism includes a slider that is slidably mounted on a fixed plate, a fixed frame that is fixed on the side wall of the slider, a sample dispensing hopper that is fixed on the top of the fixed frame, and a control component that can control the sample to be accurately dispensed into the reagent tube at the bottom of the sample dispensing hopper.
[0006] Preferably, the control assembly includes a control rod movably connected to the bottom of the sample dropper, a spring fitted on the outer wall of the control rod and installed inside the sample dropper, one end of the control rod having an inclined surface extending into the sample dropper, and a sealing ball movably disposed inside the sample dropper that can contact one end of the control rod.
[0007] Preferably, the reagent tube is fitted with a cap at the opening.
[0008] Preferably, a partition is fixed inside the box body, and two ribs fixed to the inner wall of the box body are provided on one side of the partition. A tray is placed on the ribs, and a storage groove is provided on the tray. Two connecting straps are fixed on the tray.
[0009] Preferably, the slider has an adjusting bolt threaded onto its side wall, and the adjusting bolt is inserted into an adjusting hole opened on the side wall of the fixing frame.
[0010] Preferably, the area on the front of the box corresponding to the bottom of the reagent tube is provided with a transparent window.
[0011] By employing the above technical solution, this utility model provides an EGFR gene mutation multiplex detection kit, which has at least the following beneficial effects:
[0012] 1. This EGFR gene mutation multiplex detection kit uses a dropper mechanism to pour the sample into the dropper. Then, the dropper is moved above the corresponding reagent tube. Under the control of the control component, the sample can be controlled to be dropped into the reagent tube in a droplet shape, thereby accurately controlling the amount of sample poured in and avoiding false negative or false positive results caused by uneven sample pouring.
[0013] 2. This EGFR gene mutation multiplex detection kit, by setting up partitions and trays, allows other sample collection containers or tools to be placed inside the kit, and keeps them separate from the reagent tubes, thus avoiding collision damage to the reagent tubes. Attached Figure Description
[0014] The accompanying drawings, which are included to provide a further understanding of the present invention, form part of this application:
[0015] Figure 1 This is a three-dimensional structural diagram of the entire utility model;
[0016] Figure 2 This is a schematic diagram of the structure of each component on the fixing plate of this utility model;
[0017] Figure 3 This is a cross-sectional view of the bottom of the sample dropper of this utility model;
[0018] Figure 4 This is a schematic diagram of the internal partition and tray of the box body of this utility model;
[0019] Figure label:
[0020] 1. Box body; 2. Box lid; 3. Fixing plate; 4. Sponge ring; 5. Reagent tube; 6. Tube cap; 7. Sample tube; 8. Dispensing mechanism; 801. Slider; 802. Fixing frame; 803. Adjusting bolt; 804. Dispensing hopper; 805. Control component; 8051. Spring; 8052. Control rod; 8053. Sealing ball; 9. Partition; 10. Rib; 11. Support plate; 12. Storage slot; 13. Connecting strap; 14. Transparent window. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0022] GFR gene mutations refer to changes in the epidermal growth factor receptor (EGFR) gene that may affect the function of the EGFR protein. In some types of cancer, particularly non-small cell lung cancer (NSCLC), EGFR mutations are closely associated with tumor development, progression, and response to treatment.
[0023] Example 1:
[0024] Due to the technical defects of the existing technology, the sample is not poured evenly into each reagent tube 5. Please refer to... Figures 1-4 This invention provides an EGFR gene mutation multiplex detection kit, which can control the sample to be dropped into the reagent tube 5, thereby accurately controlling the amount of sample poured in and avoiding false negative or false positive results caused by uneven sample pouring. The kit includes a box body 1 and a box cover 2 connected to the box body 1. A fixing plate 3 is fixed inside the box body 1. The fixing plate 3 has multiple placement holes one and one placement hole two. A sponge ring 4 is fixed inside the placement hole one, and a reagent tube 5 containing a fluorescently labeled probe is placed inside the sponge ring 4. A sample tube 7 is placed inside the placement hole two. A sample dispensing mechanism 8 is also slidably installed on the fixing plate 3 to add the sample to the reagent tube 5 in a drop-like manner. First, a sample is taken through the sample tube 7. After taking the sample, the sample is poured into the sample dispensing mechanism 8. Then, the sample dispensing mechanism 8 is matched with each reagent tube 5 one by one to accurately drop the sample into the reagent tube 5 for fluorescence reaction.
[0025] It should also be noted that at least five reagent tubes 5 are set up, and each fluorescently labeled probe is used to detect exon 20 mutation, exon 19 deletion, L861Q mutation, exon 18 mutation and exon 21 mutation, respectively.
[0026] To ensure accurate sample addition, please refer to... Figure 2 The sample dispensing mechanism 8 includes a slider 801 slidably mounted on a fixed plate 3. A fixed frame 802 is fixed on the side wall of the slider 801. A sample dispensing hopper 804 is fixed on the top of the fixed frame 802. A control component 805 is provided at the bottom of the sample dispensing hopper 804 to control the accurate dispensing of the sample into the reagent tube 5. Under the action of the slider 801, the sample dispensing hopper 804 can move horizontally along the fixed plate 3 to add the sample into each reagent tube 5. Under the action of the sample dispensing hopper 804, the sample can be dripped into the reagent tube 5, thereby achieving accurate sample dispensing.
[0027] To achieve droplet-like sample droplets, please refer to... Figure 3 The control component 805 includes a control rod 8052 movably connected to the bottom of the sample dropper 804. A spring 8051 is fitted on the outer wall of the control rod 8052 and installed inside the sample dropper 804. One end of the control rod 8052 has an inclined surface and extends into the sample dropper 804. A sealing ball 8053 is movably disposed inside the sample dropper 804 and can contact one end of the control rod 8052. When the control rod 8052 is pressed, the spring 8051 is compressed. Since one end of the control rod 8052 is inclined, after one end of the control rod 8052 contacts the sealing ball 8053, it can push the sealing ball 8053 upward. At this time, a gap is formed between the sealing ball 8053 and the inner wall of the sample dropper 804. The sample flows down through the gap. Since the gap is small, it can be ensured that the sample is added in a droplet manner.
[0028] To prevent leakage of the fluorescently labeled probe, a cap 6 is connected to the opening of reagent tube 5.
[0029] To facilitate the storage of the sample dropper 804, an adjusting bolt 803 is threaded onto the side wall of the slider 801. The adjusting bolt 803 is inserted into the adjusting hole opened on the side wall of the fixing frame 802. When not in use, the sample dropper 804 can be positioned in the sample tube 7. At this time, the bottom of the sample dropper 804 is stored inside the sample tube 7, which reduces the overall space occupied and thus reduces the volume of the box 1.
[0030] To facilitate observation of the fluorescence reaction inside reagent tube 5, a transparent window 14 is provided on the front of the box 1 in the area corresponding to the bottom of reagent tube 5.
[0031] Example 2:
[0032] To facilitate the placement of other sample collection containers or tools, a partition 9 is fixed inside the box body 1. Two ribs 10 are fixed to the inner wall of the box body 1 on one side of the partition 9. A tray 11 is placed on the ribs 10, and a storage slot 12 is provided on the tray 11. Two connecting straps 13 are fixed on the tray 11. By setting up the partition 9 and the tray 11, other sample collection containers or tools can be placed inside the box body 1 and separated from the reagent tube 5, which can avoid collision damage to the reagent tube 5. The design of the connecting straps 13 also makes it easy to remove the tray 11.
[0033] It should be noted that the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.
[0034] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. An EGFR gene mutation multiplex detection kit, comprising a housing (1) and a lid (2) connected to the housing (1), characterized in that: The box body (1) is fixedly provided with a fixing plate (3), which has multiple placement holes one and one placement hole two. A sponge ring (4) is fixedly provided in the placement hole one, and a reagent tube (5) containing a fluorescently labeled probe is provided in the sponge ring (4). A sample tube (7) is provided in the placement hole two. A sample dispensing mechanism (8) for adding the sample in drop form to the reagent tube (5) is also slidably provided on the fixing plate (3). The sample dispensing mechanism (8) includes a slider (801) slidably disposed on a fixed plate (3), a fixed frame (802) fixed on the side wall of the slider (801), a sample dispensing bucket (804) fixed on the top of the fixed frame (802), and a control component (805) for accurately dispensing the sample into the reagent tube (5) at the bottom of the sample dispensing bucket (804).
2. The EGFR gene mutation multiplex detection kit according to claim 1, characterized in that: The control assembly (805) includes a control rod (8052) movably connected to the bottom of the sample dropper (804). A spring (8051) installed inside the sample dropper (804) is sleeved on the outer wall of the control rod (8052). One end of the control rod (8052) has an inclined surface and extends into the sample dropper (804). A sealing ball (8053) that can contact one end of the control rod (8052) is movably arranged inside the sample dropper (804).
3. The EGFR gene mutation multiplex detection kit according to claim 1, characterized in that: The reagent tube (5) is connected to a cap (6) at its opening.
4. The EGFR gene mutation multiplex detection kit according to claim 1, characterized in that: The box body (1) is fixedly provided with a partition (9). Two ribs (10) are fixedly connected to the inner wall of the box body (1) on one side of the partition (9). A tray (11) is placed on the ribs (10). A storage groove (12) is provided on the tray (11). Two connecting straps (13) are fixedly provided on the tray (11).
5. The EGFR gene mutation multiplex detection kit according to claim 1, characterized in that: The slider (801) has an adjusting bolt (803) threadedly connected to its side wall. The adjusting bolt (803) is inserted into an adjusting hole opened on the side wall of the fixing frame (802).
6. The EGFR gene mutation multiplex detection kit according to claim 1, characterized in that: A transparent window (14) is provided on the front of the box (1) in the area corresponding to the bottom of the reagent tube (5).