Integrated anaerobic solution preparation system

Abstract

The utility model discloses an integrated anaerobic solution preparation system, which comprises a water filling component, an air inflation component and an air exhaust component which are respectively communicated with an anaerobic culture container through pipelines, the water filling component is used for providing anaerobic water for the anaerobic culture container, the air inflation component is used for providing an air source for the anaerobic culture container, and the air exhaust component is used for exhausting air from the anaerobic culture container. The air extraction assembly is used for extracting oxygen from the anaerobic culture container, the water filling assembly, the air inflation assembly and the air extraction assembly are all connected with the control device, and the control device is connected with the power supply. According to the integrated anaerobic solution preparation system provided by the utility model, a digestion method and a gas extraction method are combined, the integration of the digestion method and the gas extraction method is realized, oxygen in the solution is removed by the digestion method, and air in the container is removed by the gas extraction method by adopting a one-suction and one-feeding method; and then oxygen-free gas is injected to reach an anaerobic state. The operation is simple, the cost is low, the time and the cost are saved, and the culture requirements of anaerobic microorganisms can be effectively met.

Description

Technical Field

[0001] This utility model relates to the field of anaerobic culture container preparation, and in particular to an integrated anaerobic solution preparation system. Background Technology

[0002] In the fields of microbiology and bioengineering, the cultivation of anaerobic microorganisms is a crucial step. Due to the sensitivity of anaerobic microorganisms to oxygen, their cultivation methods are highly demanding, especially requiring a specific anaerobic environment. Currently, traditional anaerobic cultivation methods are relatively limited, specifically including the following: 1. Gas extraction method: Using a vacuum pump and gas source, air is removed from the container through a suction and injection process, followed by the injection of oxygen-free gas to achieve a vacuum state. The disadvantages are: limited by the container size, unsuitable for large-scale preparation, only suitable for smaller containers, and this method only removes oxygen from the bottle, not the solution itself, failing to achieve 100% anaerobic conditions. 2. Digestion method: The prepared reagent solution is heated to boiling for an extended period to remove oxygen from the solution, while simultaneously introducing oxygen-free gas to expel oxygen from the bottle. The disadvantages are: only one solution can be prepared at a time, reagent changes are cumbersome, and prolonged boiling leads to water loss, unclear reagent concentration, and changes in reagent composition, severely impacting experimental results.

[0003] In view of the shortcomings of the above methods, there is an urgent need in the field to develop an integrated, simple and efficient anaerobic culture container preparation system based on the combination of digestion and gas extraction methods, which would have important practical significance and application value for various biological experiments. Summary of the Invention

[0004] The purpose of this invention is to provide an integrated anaerobic solution preparation system to solve the problems existing in the prior art. It can realize an integrated anaerobic solution preparation system based on the combination of digestion and gas extraction methods, which can ensure anaerobic precision, achieve accurate control, and is simple and efficient.

[0005] To achieve the above objectives, this utility model provides the following solution:

[0006] This invention provides an integrated anaerobic solution preparation system, comprising a water filling component, an air filling component, and an air extraction component, all connected to pipelines of an anaerobic culture container. The water filling component is used to provide anaerobic water to the anaerobic culture container, the air filling component is used to provide an air source to the anaerobic culture container, and the air extraction component is used to extract oxygen from the anaerobic culture container. The water filling component, the air filling component, and the air extraction component are all connected to a control device, which is connected to a power source.

[0007] Preferably, the anaerobic culture container is an anaerobic bottle.

[0008] Preferably, the water filling assembly includes a water storage tank and a water pump connected in a water filling pipeline. The water pump is used to draw water from the water storage tank to supply water to the anaerobic bottle. A water filling pipeline solenoid valve is provided in the water filling pipeline and the water filling pipeline solenoid valve is connected to the control device.

[0009] Preferably, the inflation assembly includes an air source and a dynamic gas distribution device connected to the inflation pipeline. The dynamic gas distribution device is used to configure the gas ratio in the air source. An inflation pipeline solenoid valve is provided on the inflation pipeline and is connected to the control device.

[0010] Preferably, the gas source includes one or more of nitrogen, hydrogen, and carbon dioxide.

[0011] Preferably, the gas extraction assembly includes a vacuum pump connected to the gas extraction pipeline, the vacuum pump being used to extract gas from the anaerobic bottle, and the gas extraction pipeline being equipped with a gas extraction pipeline solenoid valve, which is connected to the control device.

[0012] Preferably, an inflation pressure gauge is provided on the inflation pipeline, and an air extraction pressure gauge is provided on the air extraction pipeline. Both the inflation pressure gauge and the air extraction pressure gauge are connected to the control device.

[0013] Preferably, the water filling pipeline and the air filling pipeline are connected to each other before being connected to the anaerobic bottle to form a water filling and air filling pipeline. The end of the water filling and air filling pipeline is connected to the bottom of the anaerobic bottle, and the end of the air extraction pipeline is connected to the mouth of the anaerobic bottle.

[0014] Preferably, the control device includes a computer controller, which is connected to a fuse and a dry-burn protection device.

[0015] Preferably, the computer controller is connected to a temperature sensor, a water pump discharge controller, a gas configuration controller, an intermittent continuous heating and insulation controller, and a gas emission controller, and the computer controller is equipped with an operation panel.

[0016] The present invention achieves the following beneficial technical effects compared to the prior art:

[0017] 1. The integrated anaerobic solution preparation system provided by this utility model combines digestion and gas extraction methods, achieving a unified approach. Digestion removes oxygen from the solution, while gas extraction, using a single intake and intake method, removes air from the container before injecting oxygen-free gas to achieve an anaerobic state. This system is simpler to operate, lower in cost, saves experimental time and costs, and effectively meets the cultivation needs of anaerobic microorganisms outside of anaerobic workstations.

[0018] 2. The integrated anaerobic solution preparation system provided by this utility model, by setting up a computer controller, can input the parameters for preparing the anaerobic solution into the computer, and control the computer through the control panel, thereby realizing precise control of various input parameters for preparing the anaerobic solution, ensuring the anaerobic state and corresponding concentration parameters of the anaerobic solution.

[0019] 3. The integrated anaerobic solution preparation system provided by this utility model uses a bottom-suction method for anaerobic water input and a top-suction method for air extraction. Specifically, the end of the water filling pipe is connected to the bottom of the anaerobic bottle, and the end of the air extraction pipe is connected to the mouth of the anaerobic bottle. This better isolates the system from oxygen in the air, ensuring an anaerobic state. Furthermore, it can simultaneously create anaerobic water and an anaerobic environment; the anaerobic bottle is not in contact with the outside world, thus isolating it from oxygen. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the pipeline structure of the integrated anaerobic solution preparation system of this utility model;

[0022] Figure 2 This is a schematic diagram of the control device of the integrated anaerobic solution preparation system of this utility model.

[0023] In the diagram: 1-Anaerobic bottle, 2-Water filling pipeline, 3-Water storage tank, 4-Water pump, 5-Water filling pipeline solenoid valve, 6-Air filling pipeline, 7-Gas source, 8-Dynamic gas distribution device, 9-Air filling pipeline solenoid valve, 10-Air extraction pipeline, 11-Vacuum pump, 12-Air extraction pipeline solenoid valve, 13-Air filling pressure gauge, 14-Air extraction pressure gauge, 15-Water and air filling pipeline, 16-Computer controller, 17-Fuse, 18-Anti-dry-burning protector, 19-Temperature probe, 20-Water pump discharge controller, 21-Gas configuration controller, 22-Intermittent continuous heating and insulation controller, 23-Gas emission controller, 24-Operation panel. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0026] This invention provides an integrated anaerobic solution preparation system, such as... Figures 1-2 As shown, the system includes a water filling assembly, an aeration assembly, and an air extraction assembly, all connected to the piping of the anaerobic culture container. The water filling assembly supplies anaerobic water to the anaerobic culture container, the aeration assembly supplies gas 7 to the anaerobic culture container, and the air extraction assembly extracts oxygen from the top of the anaerobic solution in the anaerobic culture container. All three assemblies are connected to a control device, which is connected to a power source. The anaerobic culture container is an anaerobic bottle 1.

[0027] The integrated anaerobic solution preparation system provided by this utility model, in its specific application, involves: First, a water-filling component provides anaerobic water via a digestion process for immediate use; second, a gas source 7 is provided to the anaerobic bottle 1, which has been pre-filled with reagents, via a gas-filling component, while anaerobic water is supplied to the anaerobic bottle 1 via the water-filling component; third, a gas extraction component removes residual oxygen from the top of the anaerobic solution in the anaerobic bottle 1. By employing a gas extraction method, an absolutely anaerobic state is achieved within the anaerobic bottle 1, ensuring anaerobic precision. This device combines digestion and gas extraction methods, achieving an integrated approach. Digestion removes oxygen from the solution, while gas extraction, using a one-inhale-one-out method, removes air from the container before injecting oxygen-free gas to achieve an anaerobic state.

[0028] In this embodiment, the water filling assembly includes a water reservoir 3 and a water pump 4 connected to the water filling pipeline 2. The water pump 4 is used to extract water from the water reservoir 3 to supply water to the anaerobic bottle 1. A water filling pipeline solenoid valve 5 is provided on the water filling pipeline 2 and is connected to the control device. The gas filling assembly includes a gas source 7 and a dynamic gas distribution device 8 connected to the gas filling pipeline. The dynamic gas distribution device 8 is used to configure the gas ratio in the gas source 7. A gas filling pipeline solenoid valve 9 is provided on the gas filling pipeline and is connected to the control device. The air extraction assembly includes a vacuum pump 11 connected to the air extraction pipeline 10. The vacuum pump 11 is used to extract oxygen from the anaerobic solution above the anaerobic solution in the anaerobic bottle 1. An air extraction pipeline solenoid valve 12 is provided on the air extraction pipeline 10 and is connected to the control device.

[0029] Specifically, water tank 3 is a 1.8-liter water container, and the experimental method for preparing the anaerobic solution is as follows:

[0030] 1. Fill a 1.8-liter water tank with distilled water. The automatic heating system will boil the distilled water every 10 seconds after it reaches 100 degrees Celsius. After heating for 5 minutes, the system will automatically stop heating and maintain a constant temperature of 95 degrees Celsius for later use. Boiling will bring the distilled water to an anaerobic state, thus obtaining anaerobic water.

[0031] 2. Before preparing the anaerobic solution, put the reagent into anaerobic bottle 1 and seal it.

[0032] 3. According to experimental requirements, the gas configuration settings of the gas source 7 can be adjusted through the dynamic gas distribution device 8, which can be a single gas or a mixture of gases;

[0033] 4. Open the solenoid valve 9 of the inflation line to input the prepared gas source 7 into the anaerobic bottle 1 through the inflation line. The discharge time can be set to ensure that an appropriate amount of gas source 7 is input into the anaerobic bottle 1. At the same time, open the solenoid valve 12 of the suction line to extract the oxygen above the anaerobic solution in the anaerobic bottle 1 through the vacuum pump 11, so as to realize the gas exchange of oxygen in the anaerobic bottle 1 and achieve the anaerobic state in the anaerobic bottle 1.

[0034] 5. Open the solenoid valve 5 of the water filling pipeline, and at the same time close the solenoid valve 9 of the air filling pipeline and open the solenoid valve 12 of the air extraction pipeline. Anaerobic water is introduced into the anaerobic bottle 1 through the water filling pipeline 2 and the water pump 4. The amount of anaerobic water introduced can be selected in the range of 10ml-2000ml according to the experimental requirements.

[0035] 6. After adding an appropriate amount of anaerobic water to anaerobic bottle 1, close the water filling pipeline solenoid valve 5, and simultaneously open the air filling pipeline solenoid valve 9 and the air extraction pipeline solenoid valve 12. Continue for 20 seconds.

[0036] In this anaerobic solution preparation system: 1. It is not limited by the container of the equipment itself; different reagents only need to be placed in the anaerobic bottle 1 to prepare the corresponding anaerobic solution culture medium; 2. This anaerobic solution preparation system is no longer limited by the liquid dosage and can prepare anaerobic solutions in the range of 10-2000ml; 3. This anaerobic solution preparation system adopts an integrated gas exchange and distilled water digestion and deoxygenation method, ensuring that the prepared solution is absolutely anaerobic; 4. This anaerobic solution preparation system precisely controls the output of anaerobic water through a control device to ensure the accuracy of the solution concentration; 5. Compared with the reagent solution digestion method, which increases the reagent concentration due to prolonged boiling time, this anaerobic solution preparation system can prepare anaerobic water separately, ensuring separation from the reagent and maintaining the original content of the reagent to the greatest extent; 6. By setting up a water filling pipeline solenoid valve 5, an air filling pipeline solenoid valve 9, and an air extraction pipeline solenoid valve 12, and connecting all three to the control device, precise control of water filling, air filling, and air extraction is achieved.

[0037] In this embodiment, the gas source 7 includes one or more of nitrogen, hydrogen, and carbon dioxide. This invention is not limited to this; those skilled in the art can use other types of gas sources according to actual needs.

[0038] In this embodiment, an inflation pressure gauge 13 is provided on the inflation pipeline, and an exhaust pressure gauge 14 is provided on the exhaust pipeline 10. Both the inflation pressure gauge 13 and the exhaust pressure gauge 14 are connected to a control device. By setting the inflation pressure gauge 13 and the exhaust pressure gauge 14, the air pressure can be adjusted in real time to maintain air pressure balance, remove oxygen from the anaerobic bottle 1, and achieve an absolutely anaerobic state inside the anaerobic bottle 1.

[0039] In this embodiment, the water filling pipeline 2 and the air filling pipeline are interconnected before being connected to the anaerobic bottle 1 to form a water filling and air filling pipeline 15. The end of the water filling and air filling pipeline 15 is connected to the bottom of the anaerobic bottle 1, meaning the anaerobic water is fed in via a downward suction method. The end of the air extraction pipeline 10 is connected to the mouth of the anaerobic bottle 1, meaning the air extraction is performed via an upward suction method. In this anaerobic solution preparation system, it can better maintain isolation from oxygen in the air, ensuring an anaerobic state. Moreover, it can simultaneously create anaerobic water and an anaerobic environment; the anaerobic bottle 1 is not in contact with the outside world and can isolate oxygen.

[0040] In this embodiment, the control device includes a computer controller 16, which is connected to a fuse 17 and a dry-burning protector 18. By setting the fuse 17 and the dry-burning protector 18, the water storage tank 3 is prevented from being damaged due to excessive heating, thus providing dual protection for the water storage tank 3 and preventing the occurrence of fire.

[0041] In this embodiment, the computer controller 16 is connected to the temperature probe 19, the water pump discharge controller 20, the gas preparation controller 21, the intermittent continuous heating and insulation controller 22, and the gas emission controller 23. The computer controller 16 is equipped with an operation panel 24. The temperature probe 19 is used to detect the temperature of the distilled water in the water tank 3, maintaining the distilled water within a set temperature range. The water pump discharge controller 20 allows the operator to control the output of anaerobic water according to the required amount. The gas preparation controller 21 allows the operator to control the proportion of gases required for the experiment. The intermittent continuous heating and insulation controller 22 allows the operator to control the intermittent heating of the water tank 3 to maintain the distilled water temperature between 95-99°C, thereby removing oxygen from the distilled water. The gas emission controller 23 controls the flow rate of gas in the gas filling and extraction pipelines 10.

[0042] By setting up the computer controller 16, the parameters for preparing the anaerobic solution can be input into the computer. The computer can be controlled through the operation panel 24, which realizes precise control of various input parameters for preparing the anaerobic solution, ensuring the anaerobic state and corresponding concentration parameters of the anaerobic solution, and effectively meeting the cultivation needs of anaerobic microorganisms outside the anaerobic workstation.

[0043] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. An integrated anaerobic solution preparation system, characterized in that: It includes a water filling component, an air filling component, and an air extraction component, which are respectively connected to the pipeline of the anaerobic culture container. The water filling component is used to provide anaerobic water to the anaerobic culture container, the air filling component is used to provide an air source to the anaerobic culture container, and the air extraction component is used to extract oxygen from the anaerobic culture container. The water filling component, the air filling component, and the air extraction component are all connected to a control device, and the control device is connected to a power source.

2. The integrated anaerobic solution preparation system according to claim 1, characterized in that: The anaerobic culture container is an anaerobic bottle.

3. The integrated anaerobic solution preparation system according to claim 2, characterized in that: The water filling assembly includes a water storage tank and a water pump connected in a water filling pipeline. The water pump is used to draw water from the water storage tank to supply water to the anaerobic bottle. A water filling pipeline solenoid valve is provided in the water filling pipeline and the water filling pipeline solenoid valve is connected to the control device.

4. The integrated anaerobic solution preparation system according to claim 3, characterized in that: The inflation assembly includes an air source and a dynamic gas distribution device connected to the inflation pipeline. The dynamic gas distribution device is used to configure the gas ratio in the air source. An inflation pipeline solenoid valve is provided on the inflation pipeline and is connected to the control device.

5. The integrated anaerobic solution preparation system according to claim 4, characterized in that: The gas source includes one or more of nitrogen, hydrogen, and carbon dioxide.

6. The integrated anaerobic solution preparation system according to claim 5, characterized in that: The gas extraction assembly includes a vacuum pump connected to the gas extraction pipeline, the vacuum pump being used to extract gas from the anaerobic bottle, and a gas extraction pipeline solenoid valve being provided on the gas extraction pipeline, the gas extraction pipeline solenoid valve being connected to the control device.

7. The integrated anaerobic solution preparation system according to claim 6, characterized in that: An inflation pressure gauge is installed on the inflation line, and an air extraction pressure gauge is installed on the air extraction line. Both the inflation pressure gauge and the air extraction pressure gauge are connected to the control device.

8. The integrated anaerobic solution preparation system according to claim 7, characterized in that: The water filling pipeline and the air filling pipeline are connected to each other before being connected to the anaerobic bottle to form a water filling and air filling pipeline. The end of the water filling and air filling pipeline is connected to the bottom of the anaerobic bottle, and the end of the air extraction pipeline is connected to the mouth of the anaerobic bottle.

9. The integrated anaerobic solution preparation system according to claim 1, characterized in that: The control device includes a computer controller, which is connected to a fuse and a dry-burn protection device.

10. The integrated anaerobic solution preparation system according to claim 9, characterized in that: The computer controller is connected to the temperature probe, the water pump discharge controller, the gas configuration controller, the intermittent continuous heating and heat preservation controller, and the gas emission controller. The computer controller is equipped with an operation panel.

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