Filtering and sterilizing device for culture solution

Abstract

The utility model discloses a culture solution filtering and sterilizing device, and relates to the technical field of cell sterile culture. The culture solution filtering and sterilizing device comprises a solution storage bottle used for storing a culture solution; the high-capacity injector is used for injecting a culture solution into the liquid storage bottle; the filter is fixedly connected to a liquid outlet of the high-capacity injector and is used for filtering the extruded culture solution; the filter is integrally mounted at the bottom of the high-capacity injector, direct filtration can be performed during extrusion, pollution caused by repeated disassembly and assembly can be avoided, the bottle cap capable of being unscrewed is mounted at the bottom of the filter, the bottle opening of the liquid storage bottle can be covered during injection, the pollution rate is reduced, sufficient culture liquid can be injected at a time by using the high-capacity injector, and the operation is simple and convenient. Before use, the piston is located at the opening of the syringe and can be taken down after being slightly pulled out, dust can be prevented from being sucked into the filter, sterile operation in the whole process is guaranteed, and the inspection result is more accurate.

Description

Technical Field

[0001] This utility model relates to the field of sterile cell culture technology, specifically a culture medium filtration and sterilization device. Background Technology

[0002] Aseptic cell culture is a technique that allows cells to survive, grow, multiply, and maintain their main structures and functions in vitro, mimicking the in vivo environment. This technique is of great significance for biological research and applications, particularly in cell biology, medicine, and drug development. The culture medium needs to be aseptically prepared and properly filtered before use.

[0003] Aseptic cell culture must be performed in a biosafety cabinet. Currently, researchers often use syringes and needle filters to filter and sterilize the culture medium. The procedure involves first drawing up the culture medium with a syringe, then attaching the filter, pressing the syringe to suspend the culture medium in the air and add it to a collection bottle, then removing the filter and drawing up the culture medium again. This process is often repeated several times, is tedious and complex, and is highly susceptible to contamination during repeated filter assembly. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a culture medium filtration and sterilization device, which solves the aforementioned problems.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a culture medium filtration and sterilization device, comprising:

[0006] Storage bottles are used to store culture media;

[0007] A large-capacity syringe is used to inject culture medium into a storage bottle;

[0008] A filter, fixedly connected to the outlet of a large-capacity syringe, is used to filter the extruded culture medium.

[0009] The cap can be unscrewed and fixedly connected to the bottom of the filter for screwing onto the mouth of the liquid storage bottle.

[0010] Replaceable cap, with an additional feature for sealing the reservoir bottle opening after the unscrewable cap is removed.

[0011] Preferably, the filter consists of a housing and a filter membrane disposed inside it.

[0012] Preferably, both the screw-on cap and the replaceable cap have an outer edge, and a sealing film is pasted on the outer edge surface of both the screw-on cap and the replaceable cap before use.

[0013] Preferably, the high-capacity syringe further includes a syringe barrel and a piston that slides inside it. The piston has an internal support, and a T-shaped handle is mounted on the internal support.

[0014] Preferably, a threaded sleeve is provided at the center of the top of the inner support, and the bottom end of the T-shaped handle is provided with an external thread that matches the threaded sleeve.

[0015] Preferably, the top of the inner support is provided with two rows of brackets, and the bottom and both sides of the vertical section of the T-shaped handle are provided with protruding rods.

[0016] Preferably, the bracket has an internal groove that matches the protruding rod, and an outer edge structure is provided on the outer side of one end of the bracket, with the protruding rod at the bottom of the T-shaped handle slidably disposed in the groove.

[0017] Preferably, when the protrusion at the end of the T-shaped handle slides to the end of the groove away from the outer edge structure, and the protrusion in the middle of the T-shaped handle is locked at the bottom of the outer edge structure, the T-shaped handle is restricted to a horizontal state and the piston is restricted from sliding down.

[0018] This invention provides a culture medium filtration and sterilization device. Compared with the prior art, it has the following advantages:

[0019] Beneficial effects:

[0020] 1. This culture medium filtration and sterilization device integrates a filter at the bottom of a large-capacity syringe, allowing for direct filtration during dispensing and avoiding contamination caused by repeated disassembly. A screw-on cap at the bottom of the filter covers the reservoir bottle opening during injection, further reducing contamination. The large-capacity syringe allows for a single injection of sufficient culture medium, eliminating the need for multiple extractions. This convenient design also reduces contamination. Before use, the piston is located at the syringe opening; simply pulling it out slightly removes the filter, preventing dust from being drawn in and ensuring aseptic operation throughout the process, resulting in more accurate test results.

[0021] 2. This culture medium filtration and sterilization device, by setting a bracket on the inner support and slidably setting the T-shaped handle on the inner support, allows the T-shaped handle to push and pull the piston while also laying the T-shaped handle flat, thereby locking the piston in the mouth of the syringe to prevent it from sliding down. It also facilitates the storage of the T-shaped handle. Compared with Embodiment 1, there is no need to assemble the T-shaped handle separately, making it convenient to use. Attached Figure Description

[0022] Figure 1 This is a perspective view of the structure of this utility model;

[0023] Figure 2 This is a front view of the structure of this utility model;

[0024] Figure 3Exploded view of the syringe, filter and screw-open bottle cap of this utility model;

[0025] Figure 4 This is a schematic diagram of the replaceable bottle cap of this utility model;

[0026] Figure 5 This is a schematic diagram of the sealing film and the screw-on cap of this utility model;

[0027] Figure 6 This is a schematic diagram of the connection between the piston and the T-shaped handle in Embodiment 1 of this utility model;

[0028] Figure 7 This is a schematic diagram of the connection between the piston and the T-shaped handle in Embodiment 2 of this utility model;

[0029] Figure 8 This is a schematic diagram of the T-shaped handle in a horizontal state connection according to Embodiment 2 of this utility model.

[0030] In the diagram: 1-Reservoir bottle, 2-Large-capacity syringe, 21-Injection barrel, 22-Piston, 23-Inner support, 24-T-handle, 25-Screw sleeve, 26-Bracket, 27-Protruding rod, 28-Slide groove, 29-Outer edge structure, 3-Filter, 4-Sealing membrane, 5-Unscrewable cap, 6-Replaceable cap. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] This utility model provides two technical solutions:

[0033] Figures 1-6 A first embodiment is shown: a culture medium filtration and sterilization device, comprising:

[0034] Storage bottle 1 is used to store culture medium. The bottom of storage bottle 1 is equipped with a rubber pad to prevent slipping, and the surface of storage bottle 1 is marked with graduations.

[0035] Large-capacity syringe 2 is used to inject culture medium into the storage bottle 1. The capacity of large-capacity syringe 2 is set to 100ml or 200ml.

[0036] Filter 3 is fixedly connected to the outlet of the large-capacity syringe 2 to filter the extruded culture medium; Filter 3 consists of an outer shell and a filter membrane inside it, and the filter membrane has a micropore size of 0.22um;

[0037] The screw-on cap 5 is fixedly connected to the bottom of the filter 3 and is used to screw onto the mouth of the storage bottle 1. The screw-on cap 5 does not have a rubber ring inside, which reduces the chance of external contamination while allowing air in the storage bottle 1 to be slowly released through the gap during injection. The screw-on cap 5 should not be completely tightened during use.

[0038] The replaceable cap 6 is additionally designed to be screwed onto the mouth of the storage bottle 1 after the unscrewable cap 5 is removed to seal it. The cap has a rubber ring inside for sealing.

[0039] Both the screw-on cap 5 and the replaceable cap 6 have an outer edge, and a sealing film 4 is pasted on the outer edge surface of both the screw-on cap 5 and the replaceable cap 6 before use. The sealing film 4 can cover the screw-on cap 5 and the replaceable cap 6 to prevent dust from entering. On the other hand, when the piston 22 is pulled out to generate negative pressure on the screw-on cap 5, the sealing film 4 is elastic and can be concave to compensate for the air pressure.

[0040] The large-capacity syringe 2 also includes a syringe barrel 21 and a piston 22 that slides inside it. The piston 22 has an inner support 23 inside it, and a T-shaped handle 24 is installed on the inner support 23. A threaded sleeve 25 is provided at the center of the top of the inner support 23, and the bottom end of the T-shaped handle 24 has an external thread that is compatible with the threaded sleeve 25.

[0041] The syringe 21, the screw-on cap 5, and the filter 3 are integrated into one unit, avoiding contamination caused by repeated assembly of the filter membrane.

[0042] By integrating a filter 3 at the bottom of the large-capacity syringe 2, filtration can be performed directly during extrusion, avoiding contamination caused by repeated disassembly and assembly. A screw-on cap 5 is also installed at the bottom of the filter 3, covering the mouth of the reservoir bottle 1 during injection, reducing the contamination rate. Using the large-capacity syringe 2 allows for the injection of a sufficient amount of culture medium at once, eliminating the need for multiple extractions. This method is convenient and also reduces the contamination rate. Furthermore, before use, the piston 22 is located at the mouth of the syringe 21; it can be removed by slightly pulling it out, preventing dust from being sucked into the filter 3 and ensuring aseptic operation throughout the process, resulting in more accurate test results.

[0043] Figures 7-8The second embodiment is shown. The main difference from the first embodiment is that: the top of the inner support 23 is provided with two rows of brackets 26, and the bottom and middle sides of the vertical section of the T-shaped handle 24 are provided with protruding rods 27. The inside of the brackets 26 is provided with sliding grooves 28 that are adapted to the protruding rods 27, and an outer edge structure 29 is provided on the outer side of one end of the brackets 26. The protruding rod 27 at the bottom of the T-shaped handle 24 is slidably disposed in the sliding groove 28. The end of the sliding groove 28 near the outer edge structure 29 is slightly concave downward. When the protruding rod 27 at the end of the T-shaped handle 24 slides to the end of the sliding groove 28 away from the outer edge structure 29, and the protruding rod 27 in the middle of the T-shaped handle 24 is locked at the bottom of the outer edge structure 29, the T-shaped handle 24 is restricted to a horizontal state and the piston 22 is restricted from sliding down.

[0044] By setting a bracket 26 on the inner support 23 and slidably setting the T-shaped handle 24 on the inner support 23, the T-shaped handle 24 can push and pull the piston 22, and can also be laid flat to lock the piston 22 in the mouth of the syringe 21 to prevent it from sliding down. It is also convenient to store the T-shaped handle 24. Compared with the first embodiment, there is no need to assemble the T-shaped handle 24, which is convenient to use.

[0045] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0046] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0047] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A culture medium filtration and sterilization device, characterized in that, include: Storage bottles are used to store culture media; A large-capacity syringe is used to inject culture medium into a storage bottle; A filter, fixedly connected to the outlet of a large-capacity syringe, is used to filter the extruded culture medium. The cap can be unscrewed and fixedly connected to the bottom of the filter for screwing onto the mouth of the liquid storage bottle. Replaceable cap, with an additional feature for sealing the reservoir bottle opening after the unscrewable cap is removed.

2. The culture medium filtration and sterilization device according to claim 1, characterized in that: The filter consists of a housing and a filter membrane disposed inside it.

3. The culture medium filtration and sterilization device according to claim 1, characterized in that: Both the screw-on cap and the replaceable cap have an outer edge, and a sealing film is pasted on the outer edge surface of both the screw-on cap and the replaceable cap before use.

4. The culture medium filtration and sterilization device according to claim 1, characterized in that: The high-capacity syringe also includes a syringe barrel and a piston that slides inside it. The piston has an internal support, and a T-shaped handle is mounted on the internal support.

5. The culture medium filtration and sterilization device according to claim 4, characterized in that: A threaded sleeve is provided at the center of the top of the inner support, and the bottom of the T-shaped handle is provided with an external thread that matches the threaded sleeve.

6. The culture medium filtration and sterilization device according to claim 4, characterized in that: The top of the inner support is provided with two rows of brackets, and the bottom and middle sides of the vertical section of the T-shaped handle are provided with protruding rods.

7. The culture medium filtration and sterilization device according to claim 6, characterized in that: The bracket has an internal groove that matches the protruding rod, and an outer edge structure is provided on the outer side of one end of the bracket. The protruding rod at the bottom of the T-shaped handle is slidably disposed in the groove.

8. The culture medium filtration and sterilization device according to claim 7, characterized in that: When the protrusion at the end of the T-shaped handle slides to the end of the slide groove away from the outer edge structure, and the protrusion in the middle of the T-shaped handle is locked at the bottom of the outer edge structure, the T-shaped handle is restricted to a horizontal state and the piston is restricted from sliding down.

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