Cell programmed cooling instrument suitable for biological cell programmed cooling

Abstract

The utility model discloses a cell programmed cooling instrument suitable for biological cell programmed cooling, and relates to the technical field of cell cryopreservation, the cell programmed cooling instrument suitable for biological cell programmed cooling comprises a box body and a cover body, the cover body is rotatably covered on the upper surface of the box body; a fixing base used for fixing the discharging device is arranged in the box body, and the discharging device is located on the fixing base in a separable mode. Supporting columns for supporting are arranged on the lower surface of the box body; a temperature detection probe, a fan, a power supply and a control panel are arranged on the box body, and the temperature detection probe, the fan and the power supply are electrically connected with the control panel; the fan generates cavity negative pressure to drive external cold air to flow so that programmed cooling can be conducted in the ultralow-temperature refrigerator without liquid nitrogen, and the temperature detection probe and the control panel are adopted for monitoring the temperature and controlling the cooling process. The method is suitable for various cells needing programmed cooling, and cooling programs can be conveniently set.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell freezing field technology especially is suitable for biological cell program cooling cell program cooling instrument. BACKGROUND

[0002] Cell program cooling technology is a kind of technology for cell freezing, by controlling the cooling rate, make cell gradually adapt to low-temperature environment in slow cooling process, reduce the formation of ice crystal in cell, thereby improve the survival rate after cell freezing;Liquid nitrogen program cooling method usually adopts mechanical refrigeration or other new type refrigeration technology to realize cooling, in the process of program cooling, need to be combined with ultra-low temperature refrigerator, use program cooling instrument to suck cold air in ultra-low temperature refrigerator, then flow through the cell sample needing to freeze, and cell is cooled at appropriate rate;The cell program cooling instrument in the prior art does not realize program cooling in ultra-low temperature refrigerator without liquid nitrogen by generating cavity negative pressure of fan to drive external cold air flow, does not adopt temperature detection probe and control panel to realize temperature monitoring and control cooling process;It is not convenient to be suitable for various cells needing to carry out program cooling;Therefore, in view of this status, it is urgent to develop a kind of cell program cooling instrument suitable for biological cell program cooling to meet the needs of actual use. SUMMARY

[0003] Therefore, the utility model provides a kind of cell program cooling instrument suitable for biological cell program cooling, it realizes program cooling in ultra-low temperature refrigerator without liquid nitrogen by generating cavity negative pressure of fan to drive external cold air flow, realizes temperature monitoring and control cooling process by adopting temperature detection probe and control panel;It is suitable for various cells needing to carry out program cooling;Realize the preparation and freeze integration operation of biological cell, it is convenient to set cooling program.

[0004] To achieve the above object, the utility model adopts the following technical scheme:

[0005] A kind of cell program cooling instrument suitable for biological cell program cooling, including box and lid, the lid is rotatably closed on the upper surface of box;The fixed base for fixing the discharging device is arranged in the box, and the discharging device is detachably located on the fixed base;Supporting column for supporting is arranged on the lower surface of box;Temperature detection probe for detecting temperature, fan for circulating air, power supply for providing electric energy and control panel for controlling are arranged on the box, and the temperature detection probe, fan and power supply are electrically connected with control panel.

[0006] As a preferred scheme: the feeding device comprises a cryopreservation tube rack for placing cryopreservation tubes and a cryopreservation bag rack for placing cryopreservation bags, the upper side of the fixed base is provided with a first magnetic attraction piece, the lower side of the cryopreservation tube rack and the lower side of the cryopreservation bag rack are both provided with a second magnetic attraction piece matched with the first magnetic attraction piece, and the feeding device is detachably arranged on the fixed base through the first magnetic attraction piece and the second magnetic attraction piece.

[0007] As a preferred scheme: a plurality of placing grooves for placing cryopreservation tubes are uniformly arranged on the cryopreservation tube rack; and the upper side wall of the cryopreservation bag rack is inwardly recessed to form a containing cavity for containing cryopreservation bags.

[0008] As a preferred scheme: the rear side of the cover is rotatably arranged on the rear side of the box through a hinge, and the front side of the cover is covered with the front side of the box through a buckle structure.

[0009] As a preferred scheme: the two fans are symmetrically arranged on the two opposite side walls of the box, and the two fans are electrically connected with the control panel.

[0010] As a preferred scheme: the four support columns are arranged at the four corner positions of the lower surface of the box; and a handle convenient for gripping is arranged on the upper surface of the cover.

[0011] As a preferred scheme: the two hinges are symmetrically arranged on the two sides of the cover, and the cover is reversibly arranged on the rear side of the box through the two hinges.

[0012] Compared with the prior art, the utility model has obvious advantages and beneficial effects, specifically speaking, according to the above technical scheme, the cavity negative pressure driven by the fan realizes that liquid nitrogen is not needed for program cooling in the ultra-low temperature refrigerator, the temperature detection probe and the control panel are adopted to realize temperature monitoring and control cooling process; be applicable to various cells needing program cooling; realize the preparation and cryopreservation integrated operation of biological cells, conveniently set the cooling program, stable cooling, have the advantages that operation is simple, safe and reliable, can ensure that the cooling environment is stable; the temperature detection probe real-time feedback sample temperature information, the control panel adjusts the power of the fan according to the preset cooling program, ensures that the cell is cooled at a suitable rate.

[0013] To make the structure characteristics and functions of the utility model clearer, specific embodiments will be combined with the drawings to be described in detail. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a first perspective three-dimensional structure schematic diagram of the cell program cooling instrument for biological cell program cooling of the utility model;

[0015] Figure 2 The utility model discloses a second perspective three-dimensional structure schematic diagram suitable for biological cell program cooling cell program cooling appearance of the utility model,

[0016] Figure 3 The utility model discloses a third perspective three-dimensional structure schematic diagram suitable for biological cell program cooling cell program cooling appearance of the utility model,

[0017] Figure 4 The utility model discloses a third perspective three-dimensional structure schematic diagram suitable for biological cell program cooling cell program cooling appearance of the utility model, Figure 2 The enlarged view of M in the middle.

[0018] The drawing mark explanation is as follows:

[0019] In the drawing: 10, box body; 11, discharging device; 12, cryopreservation tube frame; 121, placing groove; 13, cryopreservation bag frame; 131, containing cavity; 14, fixed base; 15, support column; 16, temperature detection probe; 17, fan; 18, power supply; 19, control panel; 20, cover body; 21, hinge; 22, buckle structure. Specific implementation

[0020] The utility model discloses a kind of cell program cooling appearance suitable for biological cell program cooling as shown in Figures 1 to 4 The utility model discloses a kind of cell program cooling appearance suitable for biological cell program cooling, including box body 10 and cover body 20, wherein:

[0021] The cover body 20 can be rotatably closed on the upper surface of the box body 10;The box body 10 is provided with a fixed base 14 for fixing the discharging device 11, and the discharging device 11 is detachably located on the fixed base 14;The lower surface of the box body 10 is provided with a support column 15 for supporting;The box body 10 is provided with a temperature detection probe 16 for detecting temperature, a fan 17 for circulating air, a power supply 18 for providing electric energy and a control panel 19 for control, and the temperature detection probe 16, the fan 17 and the power supply 18 are electrically connected with the control panel 19.

[0022] The box body 10 is provided with an electric wire connected with the external power supply 18 and a power supply 18 switch for switching, the electric wire is designed with flat wire, which facilitates the close closure of the refrigerator door;The control panel 19 has adjusting buttons and a display screen, which realizes the functions of program selection and real-time monitoring of cooling, and buzzes to remind when the program is completed;The outer side of the temperature detection probe 16 is wrapped with aluminum alloy that can be shaped, which facilitates placement at various positions of the box body 10 and temperature detection at different positions, ensuring the temperature accuracy of the box body 10.

[0023] During the process of program cooling, it needs to be combined with an ultra-low temperature refrigerator, and the cell program cooling appearance is used to suck the cold air in the ultra-low temperature refrigerator and then flow through the discharging device 11 where the cell samples to be cryopreserved are placed.

[0024] The cavity negative pressure is generated by the fan 17 to drive the external cold air to flow, so that the liquid nitrogen is not needed in the ultra-low temperature refrigerator for programmed cooling, and the temperature detection probe 16 and the control panel 19 are adopted to realize the temperature monitoring and control cooling process; it is suitable for various cells needing programmed cooling; the preparation and cryopreservation of biological cells are integrated, the cooling program is easily set, the cooling is stable, and the operation is simple, safe and reliable, so that the stable cooling environment is ensured; the temperature detection probe 16 feeds back the temperature information of the sample in real time, the control panel 19 adjusts the power of the fan 17 according to the preset cooling program, and the cell is cooled at a suitable rate.

[0025] The feeding device 11 comprises a cryopreservation tube rack 12 for placing the cryopreservation tube and a cryopreservation bag rack 13 for placing the cryopreservation bag, the upper side of the fixed base 14 is provided with a first magnetic attraction piece, the lower side of the cryopreservation tube rack 12 and the lower side of the cryopreservation bag rack 13 are provided with a second magnetic attraction piece matched with the first magnetic attraction piece, and the feeding device 11 is detachably located on the fixed base 14 through the first magnetic attraction piece and the second magnetic attraction piece; the first magnetic attraction piece and the second magnetic attraction piece are adopted to facilitate the replacement and placement of the feeding device 11, the cryopreservation tube rack 12 and the cryopreservation bag rack 13 are adsorbed and fixed in the box body 10, the cell sample is prevented from being accidentally collided or deviated, the cooling effect is affected, and the stability is improved.

[0026] The cryopreservation tube rack 12 is uniformly provided with a plurality of placing grooves 121 for placing the cryopreservation tube; the upper side wall of the cryopreservation bag rack 13 is inwardly recessed to form a containing cavity 131 for containing the cryopreservation bag; the placing grooves 121 and the containing cavities 131 are adopted to improve the stability of sample placement.

[0027] The rear side of the cover body 20 is rotatably arranged on the rear side of the box body 10 through the hinge 21, and the front side of the cover body 20 is overlapped with the front side of the box body 10 through the buckle structure 22; the hinge 21 and the buckle structure 22 are adopted to realize the flip-over overlapping between the cover body 20 and the box body 10.

[0028] The two fans 17 are symmetrically arranged on the two opposite side walls of the box body 10, and the two fans 17 are electrically connected with the control panel 19; the two symmetrically arranged fans 17 are adopted to facilitate the stable output of the two fans, and a stable cold air flow rate is formed.

[0029] The four support columns 15 are arranged at the four corner positions of the lower surface of the box body 10, and the upper surface of the cover body 20 is provided with a handle convenient for gripping; the support columns 15 are adopted to play a supporting role and facilitate placement.

[0030] The two hinges 21 are symmetrically arranged on two sides of the cover 20, and the cover 20 is reversibly arranged on the rear side of the box body 10 through the two hinges 21.

[0031] The use method and principle of the cell program cooling instrument suitable for biological cell program cooling are as follows:

[0032] In use, the cell program cooling instrument suitable for biological cell program cooling provided by the application is connected to an external power supply, and after a normal operation of an ultra-low temperature refrigerator is combined for use, a power switch is turned on, a cooling program is set or selected through a control panel, a feeding device, i.e., a cryopreservation tube rack and a cryopreservation bag rack, is pre-cooled in the ultra-low temperature refrigerator, and a cryopreserved cell sample is placed in the cryopreservation rack for standby; a buckle structure is opened, the cover is opened, the feeding device loaded with the cryopreserved sample is placed in the cell program cooling instrument, and the feeding device will be adsorbed by the fixed base; the movable temperature detection probe is placed in a suitable position to facilitate real-time monitoring of the temperature; the cover is closed, and the buckle structure is closed; the cell program cooling instrument is placed in the ultra-low temperature refrigerator; the cooling program is started through the control panel, the door of the ultra-low temperature refrigerator is closed, and the cooling program is waited to end; after a bee sound is prompted, it is indicated that the program cooling is completed, and subsequent cryopreservation operation can be performed on the cryopreserved sample.

[0033] The design emphasis of the utility model lies in that the cavity negative pressure generated by the fan drives external cold air to flow to realize program cooling in the ultra-low temperature refrigerator without liquid nitrogen, the temperature detection probe and the control panel are adopted to realize monitoring and control of the cooling process; the utility model is suitable for various cells needing program cooling; the preparation and cryopreservation of biological cells are integrated, the cooling program is conveniently set, stable cooling is realized, the utility model has the advantages of simple operation, safety and reliability, and can ensure stable cooling environment; the temperature detection probe feeds back the temperature information of the sample in real time, the control panel adjusts the power of the fan according to the preset cooling program, and the cell is cooled at a suitable rate.

[0034] The above is only a preferred embodiment of the utility model, and does not limit the technical range of the utility model, so any slight modification, equivalent change and modification made according to the technical essence of the utility model to the above embodiment still belongs to the range of the technical scheme of the utility model.

Claims

1. A cell programmed cooling device suitable for programmed cooling of biological cells, characterized in that: The application relates to a temperature-controlled box, which comprises a box body and a cover body rotatably covered on the upper surface of the box body; a fixing base for fixing a feeding device is arranged in the box body, and the feeding device is detachably arranged on the fixing base; supporting columns for supporting are arranged on the lower surface of the box body; a temperature detection probe for detecting temperature, a fan for circulating air, a power supply for providing electric energy and a control panel for controlling are arranged on the box body, and the temperature detection probe, the fan and the power supply are electrically connected with the control panel.

2. The cell programming cooler suitable for biological cell programming cooling according to claim 1, characterized in that: The feeding device comprises a cryopreservation tube rack for placing cryopreservation tubes and a cryopreservation bag rack for placing cryopreservation bags; the upper side of the fixing base is provided with a first magnetic attraction element; the lower side of the cryopreservation tube rack and the lower side of the cryopreservation bag rack are provided with second magnetic attraction elements matched with the first magnetic attraction element; and the feeding device is detachably arranged on the fixing base through the first magnetic attraction element and the second magnetic attraction elements.

3. The cell programming cooler suitable for biological cell programming cooling according to claim 2, characterized in that: A plurality of placing grooves for placing cryopreservation tubes are uniformly arranged on the cryopreservation tube rack; and the upper side wall of the cryopreservation bag rack is inwardly recessed to form a containing cavity for containing cryopreservation bags.

4. The cell programming cooler suitable for biological cell programming cooling according to claim 1, characterized in that: The rear side of the cover body is rotatably arranged on the rear side of the box body through a hinge; and the front side of the cover body is covered on the front side of the box body through a buckle structure.

5. The cell programming cooler suitable for biological cell programming cooling according to claim 1, characterized in that: The fan is provided with two fans symmetrically arranged on two opposite side walls of the box body; and the two fans are electrically connected with the control panel.

6. The cell programming cooler suitable for biological cell programming cooling according to claim 1, characterized in that: The supporting columns are provided with four supporting columns arranged on four corner positions of the lower surface of the box body; and a handle for facilitating gripping is arranged on the upper surface of the cover body.

7. The cell programming cooler suitable for biological cell programming cooling according to claim 4, characterized in that: The hinge is provided with two hinges symmetrically arranged on two sides of the cover body; and the cover body is reversibly arranged on the rear side of the box body through the two hinges.

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