Micro-fluidic chip liquid absorption and magnetic bead attraction device and liquid analysis system

Abstract

The utility model discloses a microfluidic chip liquid absorption and magnetic bead attraction device and a liquid analysis system, and the device comprises a device body which is provided with a placing bayonet for placing a microfluidic chip and an injector fixing groove for fixing an injector; the liquid suction assembly enables the injector to be connected with the output port of the micro-fluidic chip, enables the input port of the micro-fluidic chip to be connected with liquid needing to flow in, and is used for adjusting the suction force of the micro-fluidic chip to the liquid needing to flow in; the magnetic bead attraction assembly is used for attracting magnetic beads, needing to flow into liquid, in the micro-fluidic chip to move; the detection assembly is used for detecting the liquid level and the magnetic bead position in the micro-fluidic chip and outputting a corresponding liquid level signal and a corresponding magnetic bead position signal; and the controller is used for receiving the liquid level signal, outputting a first driving signal to drive the liquid suction assembly to work, receiving the magnetic bead position signal and outputting a second driving signal to drive the magnetic bead attraction assembly to work. The utility model aims to realize accurate liquid suction and magnetic bead separation of the micro-fluidic chip at the same time.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic field especially relates to a kind of micro -fluidic chip liquid suction and magnetic bead suction device and liquid analysis system. BACKGROUND

[0002] With the continuous development of biotechnology, chemical analysis technology and nanotechnology, microfluidic chip as laboratory miniaturization equipment, has been widely used in chemical reaction, analysis detection, cell culture, separation and purification etc. Many fields. On the other hand, in the biomedical field, magnetic beads as a common separation and enrichment tool, commonly used in cell sorting, DNA, RNA extraction etc. Operation.

[0003] And current liquid suction and magnetic bead separation often need different equipment and operation steps, leading to complex operation, low efficiency, and difficult to realize accurate control simultaneously. SUMMARY

[0004] In view of the above deficiencies of prior art, the utility model aims at providing a kind of micro -fluidic chip liquid suction and magnetic bead suction device and liquid analysis system, to realize accurate liquid suction and magnetic bead separation to micro -fluidic chip simultaneously.

[0005] The technical scheme of the utility model is as follows:

[0006] Firstly, the utility model provides a kind of micro -fluidic chip liquid suction and magnetic bead suction device, comprising:

[0007] Device body, the device body is formed with accommodating cavity, the device body is equipped with the placement bayonet, the placement bayonet is used to place micro -fluidic chip, the device body is formed with syringe fixed groove in, for fixing syringe;

[0008] Liquid suction component, set in the device body, the liquid suction component is used to make syringe connect the output port of micro -fluidic chip, and make the input port of micro -fluidic chip connect the liquid that needs to flow in, the liquid suction component is used to adjust the suction force of micro -fluidic chip to the liquid that needs to flow in;

[0009] Magnetic bead suction component, set in the device body, and correspondingly set below the placement bayonet, the magnetic bead suction component is used to attract the magnetic bead movement in the liquid that needs to flow in micro -fluidic chip;

[0010] Detection component, set in the device body, the detection component is used to detect the liquid level and magnetic bead position in micro -fluidic chip, and output corresponding liquid level signal and magnetic bead position signal;

[0011] a controller, an input end of the controller being connected with an output end of the detecting component, an output end of the controller being connected with input ends of the liquid suction component and the magnetic bead suction component respectively, the controller being used for receiving the liquid level signal and outputting a first driving signal to drive the liquid suction component to work, and receiving the magnetic bead position signal and outputting a second driving signal to drive the magnetic bead suction component to work.

[0012] Optionally, the liquid suction component comprises:

[0013] a syringe plunger rod for connecting a syringe;

[0014] a first sliding block formed with a plunger rod placing groove for fixedly placing the syringe plunger rod;

[0015] a first screw rod connected with the first sliding block;

[0016] a first motor, a driving end of the first motor being connected with the first screw rod, an input end of the first motor being connected with an output end of the controller, the first motor being used for driving the first screw rod to drive the syringe plunger rod on the first sliding block to move to control the syringe to adjust the suction force of the microfluidic chip to the liquid to be flowed in when receiving the first driving signal output by the controller.

[0017] Optionally, the magnetic bead suction component comprises:

[0018] an electromagnet, the electromagnet being electrically connected with the controller and being correspondingly arranged below the placing socket, the electromagnet being used for working when receiving the working signal output by the controller;

[0019] a second sliding block formed with an electromagnet placing groove for placing the electromagnet;

[0020] a second screw rod connected with the second sliding block;

[0021] a second motor, a driving end of the second motor being connected with the second screw rod, an input end of the second motor being connected with an output end of the controller, the second motor being used for driving the second screw rod to drive the electromagnet on the second sliding block to move to attract the magnetic beads in the microfluidic chip to move when receiving the second driving signal output by the controller.

[0022] Optionally, the detecting component comprises:

[0023] a liquid level sensor, an output end of the liquid level sensor being connected with an input end of the controller, the liquid level sensor being used for detecting the liquid level in the microfluidic chip and outputting a corresponding liquid level signal to the controller.

[0024] Optionally, the detection assembly further comprises:

[0025] A Hall sensor, an output end of the Hall sensor being connected with an input end of the controller, the Hall sensor being used for detecting a magnetic bead position in the micro-fluidic chip and outputting a corresponding magnetic bead position signal to the controller.

[0026] Optionally, the accommodating cavity formed by the device body comprises an injector placing bin, and a top of the injector placing bin is open, the injector fixing groove being formed in the injector placing bin; the device body comprises an openable and closable injector bin cover, and the openable and closable injector bin cover is arranged at the top of the injector placing bin.

[0027] Optionally, the micro-fluidic chip liquid suction and magnetic bead suction device further comprises:

[0028] An infrared sensor, the infrared sensor being arranged in the device body, an output end of the infrared sensor being connected with an input end of the controller, the infrared sensor being used for detecting a distance between the openable and closable injector bin cover and the infrared sensor and outputting a corresponding distance detection signal to the controller.

[0029] Optionally, the micro-fluidic chip liquid suction and magnetic bead suction device further comprises:

[0030] A touch control screen, the touch control screen being connected with the controller, the touch control screen being used for outputting a control trigger signal to the controller when being triggered by a user, and the controller being used for controlling the magnetic bead suction assembly and the liquid suction assembly to work according to the control trigger signal.

[0031] Optionally, the micro-fluidic chip liquid suction and magnetic bead suction device further comprises:

[0032] A power supply circuit, an input end of the power supply circuit being used for connecting with an external power supply, output ends of the power supply circuit being connected with a power supply end of the magnetic bead suction assembly, a power supply end of the liquid suction assembly, a power supply end of the controller and a power supply end of the detection assembly, and the power supply circuit being used for converting the external power supply and providing working voltages for the magnetic bead suction assembly, the liquid suction assembly, the controller and the detection assembly.

[0033] The utility model further provides a kind of liquid analysis system, including liquid container and the micro-fluidic chip liquid suction and magnetic bead suction device as described above, the liquid container is used to accommodate the liquid that needs to flow, and the liquid container is connected with the liquid suction assembly in the micro-fluidic chip liquid suction and magnetic bead suction device.

[0034] The utility model discloses a technical scheme through the device body, liquid suction subassembly, magnetic bead attraction subassembly, detection component and controller constitute micro -fluidic chip liquid suction and magnetic bead attraction device, wherein, the device body forms and holds the cavity, and the device body is set up and placed the bayonet, and the bayonet is used for placing micro -fluidic chip, and the device body forms and has syringe fixed groove in, for fixing syringe, liquid suction subassembly sets up in the device body, and liquid suction subassembly is used for making syringe connect the output of micro -fluidic chip, and makes the input of micro -fluidic chip connect the liquid that needs to flow, and liquid suction subassembly is used for adjusting the suction of micro -fluidic chip to the liquid that needs to flow, magnetic bead attraction subassembly sets up in the device body, and correspondingly sets up below the bayonet, and magnetic bead attraction subassembly is used for attracting the magnetic bead movement in the liquid that needs to flow in micro -fluidic chip, detection component sets up in the device body, and the output of detection component is connected with magnetic bead attraction subassembly and liquid suction subassembly respectively, and detection component is used for detecting the liquid level and magnetic bead position in micro -fluidic chip, and exports corresponding liquid level signal and magnetic bead position signal, controller, the output of controller is connected with the output of detection component, and the input of liquid suction subassembly and magnetic bead attraction subassembly is connected with the output of controller respectively, and controller is used for receiving liquid level signal and exporting first drive signal and driving liquid suction subassembly work, and receiving magnetic bead position signal and exporting second drive signal and driving magnetic bead attraction subassembly work, in this way, the micro -fluidic chip liquid suction and magnetic bead attraction device in this scheme can realize the liquid suction and magnetic bead separation of micro -fluidic chip simultaneously, and through detection component can detect liquid level and magnetic bead position, and then control magnetic bead attraction subassembly and liquid suction subassembly work, adjust liquid level and magnetic bead position, thereby improve the precision of liquid suction and magnetic bead separation. BRIEF DESCRIPTION OF DRAWINGS

[0035] In order to more clearly illustrate the technical scheme in the embodiment of the utility model or prior art, below will be to the drawing needed to use in the embodiment or prior art description briefly introduce, obviously, the drawing in the following description only some embodiments of the utility model, for those skilled in the art, under the premise of not paying creative labor, can also obtain other drawings according to the structure shown in these drawings.

[0036] Figure 1 It is the function module schematic diagram of the utility model micro -fluidic chip liquid suction and magnetic bead attraction device one embodiment.

[0037] Figure 2 It is the structure schematic diagram of the utility model micro -fluidic chip liquid suction and magnetic bead attraction device one embodiment.

[0038] Figure 3 It is the structure schematic diagram of the utility model micro -fluidic chip liquid suction and magnetic bead attraction device another embodiment.

[0039] Figure 4It is another embodiment of the function module schematic view of the microfluidic chip liquid suction and magnetic bead suction device.

[0040] Figure 5 It is a structural schematic view of the microfluidic chip in the microfluidic chip liquid suction and magnetic bead suction device of the placement bayonet one embodiment.

[0041] The marker 10, the device body; 11, the placement bayonet; 111, the microfluidic chip entrance; 112, the microfluidic chip exit; 113, the microfluidic chip reaction area; 12, the syringe fixed groove; 13, the openable syringe warehouse cover; 20, the liquid suction assembly; 21, the syringe pressure rod; 22, the first sliding block; 221, the pressure rod placement groove; 23, the first screw rod; 24, the first motor; 30, the magnetic bead suction assembly; 31, the second sliding block; 32, the second screw rod; 33, the second motor; 40, the detection assembly; 41, the liquid level sensor; 42, the hall sensor; 50, the touch control screen; 60, the syringe; 70, the controller; 80, the infrared sensor; 90, the power supply circuit. DETAILED DESCRIPTION

[0042] In order to make the purpose, technical scheme and effect of the utility model more clear, definite, the following refers to the drawing and takes example to the utility model further detailedly.It should be understood that the specific embodiments described herein are only used to explain the utility model, and are not used to limit the utility model.

[0043] In the embodiment and the patent application scope, unless the article has special limitation in the text, "one", "a", "said" and "the" can also include the plural form.If the description of "first", "second" and the like is involved in the embodiments of the utility model, the description of "first", "second" and the like is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features.Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features.

[0044] It should be further understood that the phrase "comprising" used in the specification of the utility model means that the features, integers, steps, operations, elements and / or components exist, but does not exclude the existence or addition of one or more other features, integers, steps, operations, elements, components and / or their groups.It should be understood that when an element is said to be "connected" or "coupled" to another element, it can be directly connected or coupled to the other element, or there can be an intermediate element.In addition, "connection" or "coupling" used herein can include wireless connection or wireless coupling.The phrase "and / or" used herein includes all or any unit and all combinations of the associated listed items.

[0045] Those skilled in the art will understand that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

[0046] In addition, the technical solutions of various embodiments can be combined with each other, but it must be based on the fact that a person skilled in the art can realize it. When the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, and is also not within the protection scope required by the application.

[0047] With the continuous development of biotechnology, chemical analysis technology and nanotechnology, microfluidic chips as laboratory miniaturization equipment have been widely used in chemical reaction, analysis and detection, cell culture, separation and purification and other fields. On the other hand, in the field of biomedicine, magnetic beads as a common separation and enrichment tool are often used for cell sorting, DNA, RNA extraction and other operations.

[0048] At present, liquid suction and magnetic bead separation often need different equipment and operation steps, resulting in complex operation, low efficiency, and it is difficult to realize precise control at the same time.

[0049] To solve the above problems, the utility model provides a microfluidic chip liquid suction and magnetic bead suction device.

[0050] Reference Figure 1 In an embodiment, the microfluidic chip liquid suction and magnetic bead suction device comprises:

[0051] The device body 10 is formed with a containing cavity, and the device body 10 is provided with a placing bayonet 11, the placing bayonet 11 is used for placing a microfluidic chip, and the device body 10 is formed with a syringe fixing groove 12 for fixing a syringe 60;

[0052] The liquid suction assembly 20 is arranged in the device body 10, and the liquid suction assembly 20 is used for connecting the output port of the syringe 60 with the microfluidic chip and connecting the input port of the microfluidic chip with the liquid to be flowed in, and the liquid suction assembly 20 is used for adjusting the suction force of the microfluidic chip on the liquid to be flowed in;

[0053] The magnetic bead suction assembly 30 is arranged in the device body 10 and corresponds to the lower side of the placing bayonet 11, and the magnetic bead suction assembly 30 is used for attracting the magnetic beads in the liquid to be flowed in in the microfluidic chip to move;

[0054] The detection assembly 40 is arranged in the device body 10, and the output ends of the detection assembly 40 are connected with the magnetic bead attraction assembly 30 and the liquid suction assembly 20 respectively. The detection assembly 40 is used for detecting the liquid level and the magnetic bead position in the microfluidic chip, and driving the magnetic bead attraction assembly 30 and the liquid suction assembly 20 to work according to the liquid level and the magnetic bead position.

[0055] In the embodiment, the accommodation cavity is formed by the device body 10, which can be used to fix the positional relationship of the liquid suction assembly 20, the magnetic bead attraction assembly 30 and the detection assembly 40 in the device body 10, and ensure the safety and stability inside the accommodation cavity formed by the microfluidic chip liquid suction and magnetic bead attraction device. When the microfluidic chip liquid suction and magnetic bead attraction device works, the positional relationship of the liquid suction assembly 20, the magnetic bead attraction assembly 30 and the detection assembly 40 will not change. The placement aperture 11 on the device body 10 can be set according to the size of the microfluidic chip, for example, the placement aperture 11 with a size of 40mmx15mm is set, and the specific size can be set according to the actual situation. In addition, the bottom of the placement aperture 11 can be provided with an opening, so that the magnetic bead attraction assembly 30 in the device can be closer to the bottom of the microfluidic chip, thereby realizing the accurate and strong control of the magnetic beads flowing in the microfluidic chip. The syringe fixing groove 12 in the device body 10 can be used to fix the syringe 60, and the size of the syringe fixing groove 12 can be set according to the actual size of the syringe 60. By connecting the microfluidic chip with the syringe 60, the liquid suction assembly 20 can exert pressure or suction force on the syringe 60, so that the syringe 60 can exert pressure or suction force on the microfluidic chip, thereby making the microfluidic chip suck or discharge the liquid to be flowed in. The liquid to be flowed in can be accommodated in a beaker or a test tube, and the liquid suction assembly 20 can connect the liquid to be flowed in and the input port of the microfluidic chip through a hose, and also can connect the syringe 60 and the output port of the microfluidic chip through a hose. In addition, the syringe 60 can be replaced according to different situations in actual application in the embodiment, and the design of the replaceable syringe 60 can achieve the effect of preventing pollution in some biological sample experiments with pollution.

[0056] The liquid suction assembly 20 can apply pressure or suction to the microfluidic chip by a negative pressure suction device, such as a mechanical structure driven syringe 60, so that the microfluidic chip sucks in or discharges the liquid to be flowed in. The magnetic bead suction assembly 30 can be controlled by moving an electromagnet through the arrangement of the electromagnet and the mechanical structure, so as to control the movement of the magnetic beads in the liquid to be flowed in. It should be noted that the bottom of the internal space of the microfluidic chip placed on the placement socket 11 in the embodiment can have an array of million micropores, and the diameter can be 4um, which can just fall into a magnetic bead. Therefore, when the magnetic beads mixed in the liquid to be flowed in pass through the microfluidic chip, they will be more stably clamped in the micropores in the lower layer of the microfluidic chip under the attraction of the electromagnet in the magnetic bead suction assembly 30. After this step is completed, the oil droplets can be flowed in, and the oil film is formed on the micropores to ensure that only one magnetic bead is in one micropore. The structure of the microfluidic chip can refer to Figure 5 , the microfluidic chip includes a microfluidic chip inlet 111, a microfluidic chip outlet 112, and a microfluidic chip reaction area 113.

[0057] The detection assembly 40 can be composed of multiple sensors and a controller 70, such as a liquid level sensor 41 and other devices for detecting the liquid level, and a Hall element and other devices for detecting the position of the magnetic beads. The controller 70 can be a digital signal processor (Digital Signal Processor, abbreviated as DSP), a programmable logic device (Programmable Logic Device, abbreviated as PLD), a field programmable gate array (Field Programmable Gate Array, abbreviated as FPGA), a microprocessor, an MCU single-chip microcomputer, or other electronic elements. The controller 70 can determine the liquid level and the position of the magnetic beads according to the liquid level signal and the magnetic bead position signal output by the sensors, and output a first driving signal and a second driving signal to drive the liquid suction assembly 20 and the magnetic bead suction assembly 30 to work, respectively, so as to adjust the liquid level and the position of the magnetic beads in the microfluidic chip. For example, when the liquid level is zero at the end of liquid suction, the controller 70 can control the liquid suction assembly 20 to stop working. When the Hall element senses that the magnetism is stable, it means that the magnetic beads have reached the designated area, and the controller 70 can output a working signal to control the magnetic bead suction assembly 30 to stop attracting the magnetic beads. The present scheme integrates the liquid suction assembly 20 and the magnetic bead suction assembly 30 in the same space, reduces the volume of the equipment, and improves the simplicity of experimental operation.

[0058] The utility model discloses a technical scheme through device body 10, liquid suction subassembly 20, magnetic bead attraction subassembly 30 and detection subassembly 40 constitute micro -fluidic chip liquid suction and magnetic bead attraction device, wherein, device body 10 forms and holds the cavity, and device body 10 is set up and placed bayonet 11, and placed bayonet 11 is used to place micro -fluidic chip, and device body 10 is formed with syringe fixed groove 12 in, for fixing syringe 60, liquid suction subassembly 20 sets up in device body 10, and liquid suction subassembly 20 is used for making syringe 60 connect the output of micro -fluidic chip, and make the input of micro -fluidic chip connect the liquid that needs to flow, and liquid suction subassembly 20 is used for adjusting the suction of micro -fluidic chip to the liquid that needs to flow, magnetic bead attraction subassembly 30 sets up in device body 10, and correspondingly sets up below placed bayonet 11, and magnetic bead attraction subassembly 30 is used for attracting the magnetic bead movement in the liquid that needs to flow in micro -fluidic chip, detection subassembly 40 sets up in device body 10, and the output of detection subassembly 40 is connected with magnetic bead attraction subassembly 30 and liquid suction subassembly 20 respectively, and detection subassembly 40 is used for detecting the liquid level and magnetic bead position in micro -fluidic chip, and exports corresponding liquid level signal and magnetic bead position signal, the input of controller 70 is connected with the output of detection subassembly, and the output of controller 70 is connected with the input of liquid suction subassembly and magnetic bead attraction subassembly respectively, and controller 70 is used for receiving liquid level signal and exporting first drive signal and drive liquid suction subassembly work, and receiving magnetic bead position signal and exporting second drive signal and drive magnetic bead attraction subassembly work, so, the micro -fluidic chip liquid suction and magnetic bead attraction device in this scheme can realize the liquid suction and magnetic bead separation of micro -fluidic chip simultaneously, and through detection subassembly 40, the liquid level and magnetic bead position can be detected, and then control magnetic bead attraction subassembly 30 and liquid suction subassembly 20 work, adjust the liquid level and magnetic bead position, thereby improve the precision of liquid suction and magnetic bead separation.

[0059] Referring to Figure 2 and Figure 3 In an embodiment, the liquid suction subassembly 20 comprises:

[0060] A syringe plunger rod 21 is used to connect the syringe 60.

[0061] A first sliding block 22 is formed with a plunger rod placement groove 221 for fixing the syringe plunger rod 21.

[0062] A first lead screw 23 is connected with the first sliding block 22.

[0063] A first motor 24, a driving end of the first motor 24 is connected with the first screw rod 23, an input end of the first motor 24 is connected with an output end of the controller 70, the first motor 24 is used for driving the first screw rod 23 to drive the syringe plunger 21 on the first sliding block 22 to move to control the syringe 60 to adjust the suction of the microfluidic chip to the liquid to be flowed in when receiving a first driving signal output by the controller 70.

[0064] In the embodiment, the liquid suction assembly 20 can be composed of the syringe plunger 21, the first sliding block 22, the first screw rod 23 and the first motor 24, the syringe 60 can be fixed on the syringe fixing groove 12 formed in the device body 10, and the syringe plunger 21 is fixed in the plunger placing groove 221 formed in the first sliding block 22. In the embodiment, the first motor 24 can be combined with the first screw rod 23 and the first sliding block 22 to realize the accurate control of the syringe plunger 21, so as to control the suction or pressure of the syringe 60 to the microfluidic chip, so as to control the liquid suction amount of the microfluidic chip; for example, if the suction of the syringe 60 to the microfluidic chip is increased, the microfluidic chip will suck the liquid to be flowed in through the hose, if the pressure of the syringe 60 to the microfluidic chip is increased, the microfluidic chip will discharge the liquid to be flowed in through the hose, so as to control the liquid level in the microfluidic chip.

[0065] Referring to Figure 2 and Figure 3 In an embodiment, the magnetic bead suction assembly 30 comprises:

[0066] An electromagnet, electrically connected with the controller 70 and correspondingly arranged below the placing bayonet 11, the electromagnet is used for working when receiving a working signal output by the controller;

[0067] A second sliding block 31, formed with an electromagnet placing groove for placing the electromagnet;

[0068] A second screw rod 32, connected with the second sliding block 31;

[0069] A second motor 33, a driving end of the second motor 33 is connected with the second screw rod 32, an input end of the second motor 33 is connected with an output end of the controller 70, the second motor 33 is used for driving the second screw rod 32 to drive the electromagnet on the second sliding block 31 to move to attract the magnetic beads in the microfluidic chip to move when receiving a second driving signal output by the controller 70.

[0070] In the embodiment, the magnetic bead attraction assembly 30 can be composed of an electromagnet, a second sliding block 31, a second screw rod 32 and a second motor 33. The electromagnet (not shown in the figure) can be arranged at the bottom of the microfluidic chip and placed in the electromagnet placing groove on the second sliding block 31. The electromagnet can attract the magnetic beads in the fluid channel of the microfluidic chip by magnetic force. The electromagnet in the magnetic bead attraction assembly 30 in the embodiment is electrically connected with the controller 70, and can adjust the strength of the magnetic field of the electromagnet according to the working signal output by the controller 70, so as to accurately control the movement and distribution of the magnetic beads. In addition, the electromagnet module is driven by the structure of the second motor 33, the second screw rod 32 and the second sliding block 31, has high precision and stable movement, and the second motor 33 can work according to the second driving signal. The accurate control of the magnetic beads in the microfluidic chip in a small area is ensured.

[0071] With reference to Figure 4 In an embodiment, the detection assembly comprises:

[0072] A liquid level sensor 41, an output end of the liquid level sensor 41 being connected with an input end of the controller 70, the liquid level sensor 41 being used for detecting the liquid level in the microfluidic chip and outputting a corresponding liquid level signal to the controller 70.

[0073] In the embodiment, the liquid level sensor 41 is a sensor for measuring the liquid level by using the principle of capacitance change. The liquid level sensor 41 internally contains one or more fixed electrodes and a variable electrode. When the liquid level rises, the variable electrode will be immersed in the liquid, causing the total capacitance of the capacitor to change. By measuring this change, the height of the liquid level can be calculated. Specifically, a capacitive liquid level sensor, an ultrasonic liquid level sensor, a laser liquid level sensor, a photoelectric liquid level sensor, a pressure liquid level sensor or a magnetic float liquid level sensor can be used. The type of the liquid level sensor 41 can be selected according to the detection accuracy required by the type of liquid in the actual application. Therefore, the liquid level sensor 41 can be used to detect the change of the liquid level in the microfluidic chip.

[0074] With reference to Figure 4 In an embodiment, the detection assembly further comprises:

[0075] A Hall sensor 42, an output end of the Hall sensor 42 being connected with an input end of the controller 70, the Hall sensor 42 being used for detecting the position of the magnetic beads in the microfluidic chip and outputting a corresponding magnetic bead position signal to the controller 70.

[0076] In the embodiment, the basic structure of the Hall sensor 42 can include a Hall element, an amplifier and an output circuit. The Hall element is usually made of semiconductor materials such as gallium arsenide, indium antimonide, etc. When current passes through the Hall element, a magnetic field perpendicular to the current direction is generated, which deflects the carriers in the Hall element, thereby generating an electromotive force in the transverse direction. The amplifier amplifies the weak electromotive force generated by the Hall element to a measurable level and outputs it as a standard electrical signal such as voltage or current. The output circuit can convert the amplified signal into a digital signal or an analog signal, i.e. a magnetic bead position signal output to the controller 70. Therefore, the embodiment can detect the change of the magnetic bead position in the microfluidic chip by setting the Hall sensor 42.

[0077] With reference to Figure 2 In an embodiment, the accommodating cavity formed by the device body 10 includes an injector placing bin, and the top of the injector placing bin is open. The injector fixing groove 12 is formed in the injector placing bin. The device body 10 includes an openable and closable injector bin cover 13, which is arranged on the top of the injector placing bin.

[0078] In the embodiment, by opening the openable and closable injector bin cover 13, various types of injectors 60 can be replaced. By closing the openable and closable injector bin cover 13, various devices and structures of the device body 10 can be protected. The openable and closable injector bin cover 13 and the device body 10 can be connected by a hinge or other connecting device.

[0079] With reference to Figure 4 In an embodiment, the microfluidic chip liquid suction and magnetic bead suction device further includes:

[0080] An infrared sensor 80 is arranged in the device body 10. The output end of the infrared sensor 80 is connected to the input end of the controller 70. The infrared sensor 80 is used to detect the distance between the openable and closable injector bin cover 13 and the infrared sensor 80, and output a corresponding distance detection signal to the controller 70.

[0081] In the embodiment, the infrared sensor 80 is arranged to detect the distance between the openable and closable injector bin cover 13 and the infrared sensor 80, so as to determine whether the openable and closable injector bin cover 13 is closed. If the distance between the openable and closable injector bin cover 13 and the infrared sensor 80 is greater than a preset closing distance according to the distance detection signal obtained by the controller 70 in the detection assembly 40 during the working process, it can be determined that the openable and closable injector bin cover 13 is not closed. At this time, the microfluidic chip liquid suction and magnetic bead suction device can be controlled to stop working by the detection assembly 40, so as to avoid safety accidents.

[0082] In addition, in another embodiment, the microfluidic chip liquid suction and magnetic bead suction device can also be provided with a plurality of infrared sensors 80, which can respectively detect whether the syringe pressing rod 21 is stuck during operation, causing the detection assembly 40 to issue a continue operation instruction but the first sliding block 22 does not move, and by detecting the distance of the operation of the syringe pressing rod 21, the volume of the suction liquid can also be calculated in real time; in the magnetic bead suction assembly 30, whether the second sliding block 31 is stuck can be detected. In this way, the state of each structure can be detected in real time, and when any structure fails or cannot operate, the microfluidic chip liquid suction and magnetic bead suction device can be immediately controlled to stop working.

[0083] Referring to Figure 2 In an embodiment, the microfluidic chip liquid suction and magnetic bead suction device further comprises:

[0084] The touch control screen 50 is connected with the controller 70, and the touch control screen 50 is used to output a control trigger signal to the controller 70 when triggered by a user, and the controller 70 is used to control the magnetic bead suction assembly 30 and the liquid suction assembly 20 to work according to the control trigger signal.

[0085] In this embodiment, the touch control screen 50 can be a resistive touch screen, a capacitive touch screen, an infrared touch screen or an optical touch screen, etc. The touch control screen 50 combines the functions of a display and an input device, and a user can directly operate on the screen by a finger or a special touch pen, so that the touch control screen 50 outputs a control trigger signal to the controller 70, and the controller 70 can control the magnetic bead suction assembly 30 and the liquid suction assembly 20 to work according to the control trigger signal. In addition, the touch control screen 50 can also display liquid level information and magnetic bead position information, and display “completed” and other text information when the liquid suction is completed.

[0086] Referring to Figure 4 In an embodiment, the microfluidic chip liquid suction and magnetic bead suction device further comprises:

[0087] The power supply circuit 90 has an input end connected with an external power supply, and an output end connected with a power supply end of the magnetic bead suction assembly 30, a power supply end of the liquid suction assembly 20, a power supply end of the controller 70 and a power supply end of the detection assembly 40, and the power supply circuit 90 is used to convert the external power supply to provide working voltage for the magnetic bead suction assembly 30, the liquid suction assembly 20, the controller 70 and the detection assembly 40.

[0088] In the embodiment, the power supply circuit 90 can be implemented by a DC-DC circuit or a power supply chip, and the power supply circuit 90 can be directly arranged on a circuit board in the micro-fluidic chip liquid suction and magnetic bead attraction device. The power supply circuit 90 can convert an external power supply into a working voltage suitable for the magnetic bead attraction assembly 30, the liquid suction assembly 20, the controller 70 and the detection assembly 40, so as to prevent the magnetic bead attraction assembly 30, the liquid suction assembly 20, the controller 70 and the detection assembly 40 from being damaged due to receiving a higher working voltage or from being unable to normally work due to receiving a lower working voltage.

[0089] The utility model discloses still propose a kind of liquid analysis system.

[0090] In an embodiment, the liquid analysis system comprises a liquid container for containing liquid to be flowed in, and the micro-fluidic chip liquid suction and magnetic bead attraction device as described above, and the liquid container is connected with the liquid suction assembly in the micro-fluidic chip liquid suction and magnetic bead attraction device. It can be understood that, since the micro-fluidic chip liquid suction and magnetic bead attraction device as described above is used in the liquid analysis system of the utility model, the embodiments of the liquid analysis system of the utility model include all the technical solutions of all the embodiments of the micro-fluidic chip liquid suction and magnetic bead attraction device as described above, and the technical effects achieved are also exactly the same, and will not be repeated here.

[0091] It should be understood that the application of the utility model is not limited to the above examples, and those skilled in the art can improve or transform it according to the above description, and all these improvements and transformations shall belong to the protection scope of the claims attached to the utility model.

Claims

1. A microfluidic chip liquid suction and magnetic bead suction device, characterized in that, The device comprises: a device body, which is formed with a receiving cavity, and is provided with a placing aperture for placing a microfluidic chip, and is formed with a syringe fixing groove for fixing a syringe; a liquid suction assembly arranged in the device body, which is used to connect the output port of the microfluidic chip with the syringe, and connect the input port of the microfluidic chip with the liquid to be flowed in, and is used to adjust the suction force of the microfluidic chip on the liquid to be flowed in; a magnetic bead attraction assembly arranged in the device body and corresponding to the lower part of the placing aperture, which is used to attract the magnetic beads in the liquid to be flowed in in the microfluidic chip to move; a detection assembly arranged in the device body, which is used to detect the liquid level and the position of the magnetic beads in the microfluidic chip, and output corresponding liquid level signals and magnetic bead position signals; a controller, the input end of which is connected with the output end of the detection assembly, the output end of which is connected with the input end of the liquid suction assembly and the magnetic bead attraction assembly respectively, which is used to receive the liquid level signals and output first driving signals to drive the liquid suction assembly to work, and receive the magnetic bead position signals and output second driving signals to drive the magnetic bead attraction assembly to work.

2. The microfluidic chip liquid suction and magnetic bead suction device according to claim 1, wherein, The liquid suction assembly comprises: a syringe pressing rod for connecting the syringe; a first sliding block, which is formed with a pressing rod placing groove for fixing the syringe pressing rod; a first screw rod connected with the first sliding block; a first motor, the driving end of which is connected with the first screw rod, the input end of which is connected with the output end of the controller, which is used to drive the first screw rod to drive the syringe pressing rod on the first sliding block to move to control the syringe to adjust the suction force of the microfluidic chip on the liquid to be flowed in when receiving the first driving signals output by the controller.

3. The microfluidic chip liquid suction and magnetic bead suction device according to claim 1, wherein, The magnetic bead attraction assembly comprises: an electromagnet, which is electrically connected with the controller and corresponding to the lower part of the placing aperture, which is used to work when receiving the working signals output by the controller; a second sliding block, which is formed with an electromagnet placing groove for placing the electromagnet; a second screw rod connected with the second sliding block; a second motor, the driving end of which is connected with the second screw rod, the input end of which is connected with the output end of the controller, which is used to drive the second screw rod to drive the electromagnet on the second sliding block to move to attract the magnetic beads in the liquid to be flowed in in the microfluidic chip to move when receiving the second driving signals output by the controller.

4. The microfluidic chip liquid suction and magnetic bead suction device according to claim 1, wherein, The detection assembly comprises: a liquid level sensor, the output end of which is connected with the input end of the controller, which is used to detect the liquid level in the microfluidic chip and output corresponding liquid level signals to the controller.

5. The microfluidic chip liquid suction and magnetic bead suction device according to claim 1, wherein, The detection assembly further comprises: A Hall sensor, an output end of the Hall sensor being connected with an input end of the controller, the Hall sensor being used for detecting a magnetic bead position in the micro-fluidic chip and outputting a corresponding magnetic bead position signal to the controller.

6. The microfluidic chip liquid suction and magnetic bead suction device according to claim 1, wherein, The accommodating cavity formed by the device body includes a syringe placing bin, and a top of the syringe placing bin is open, and the syringe fixing groove is formed in the syringe placing bin; the device body includes an openable and closable syringe bin cover, and the openable and closable syringe bin cover is arranged at the top of the syringe placing bin.

7. The microfluidic chip liquid suction and magnetic bead suction device according to claim 6, wherein, The micro-fluidic chip liquid suction and magnetic bead suction device further includes: An infrared sensor is arranged in the device body, an output end of the infrared sensor being connected with an input end of the controller, the infrared sensor being used for detecting a distance between the openable and closable syringe bin cover and the infrared sensor and outputting a corresponding distance detection signal to the controller.

8. The microfluidic chip liquid suction and magnetic bead suction device according to claim 1, wherein, The micro-fluidic chip liquid suction and magnetic bead suction device further includes: A touch control screen is connected with the controller, the touch control screen being used for outputting a control trigger signal to the controller when being triggered by a user, and the controller being used for controlling the magnetic bead suction assembly and the liquid suction assembly to work according to the control trigger signal.

9. The microfluidic chip liquid suction and magnetic bead suction device according to claim 1, wherein, The micro-fluidic chip liquid suction and magnetic bead suction device further includes: A power supply circuit, an input end of the power supply circuit being used for connecting with an external power supply, output ends of the power supply circuit being connected with power supply ends of the magnetic bead suction assembly, the liquid suction assembly, the controller and the detection assembly, and the power supply circuit being used for converting the external power supply and providing working voltages for the magnetic bead suction assembly, the liquid suction assembly, the controller and the detection assembly.

10. A liquid analysis system characterized by comprising: The micro-fluidic chip liquid suction and magnetic bead suction device is used for connecting with a liquid container, and the liquid container is used for containing liquid to be flowed in.

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