Matrix laying device

Abstract

The utility model relates to a biomedical experimental instrument, in particular to a matrigel laying device, which comprises a placing piece (1), a laying assembly (2) and a clamping assembly (3), and is characterized in that a plurality of reaction cavities (11) are formed on the placing piece (1), and the laying assembly (2) is placed in the reaction cavities (11) or taken out from the reaction cavities (11) through the clamping assembly (3); the laying assembly (2) comprises a pressing plate (21) and a supporting piece (22), the pressing plate (21), the supporting piece (22) and the reaction cavity (11) define a containing cavity (23) capable of containing matrigel, and a gel injection channel (24) is formed in the pressing plate (21) so that the interior of the containing cavity (23) can be filled with the matrigel. The device can assist in completing matrigel laying, so that matrigel laying is uniform and flat, and the experiment precision is further improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biomedical experimental instrument, and concretely relates to a substrate glue laying device. BACKGROUND

[0002] There are some experiments in biomedical that need to use thick glue method to lay even substrate glue, such as 3D cell culture, organoid culture, cell invasion experiment etc. The role of substrate glue in thick glue method is to provide three-dimensional support structure, support cell adhesion and growth, and simulate the natural cell growth environment. Substrate glue through its complex three-dimensional network structure, similar to the microenvironment of in-vivo cell growth, helps cell culture and differentiation in vitro.

[0003] Substrate glue will begin to gel at 10 DEG C or above, at 22-37 DEG C, substrate glue macromolecules can combine with each other through chemical bonds, promote substrate glue to rapidly polymerize and form 3D gel substrate, and under the condition of a certain range of low temperature (such as 4 DEG C), substrate glue presents liquid state because there is not enough energy to promote chemical bond combination. The existing technical method is mainly to spread substrate glue with a pipette. However, because substrate glue has the characteristics of high viscosity and easy solidification, it is difficult to lay flat and even, which greatly affects the accuracy of the experiment.

[0004] Therefore, it is necessary to design a substrate glue laying device to assist the laying of substrate glue. UTILITY MODEL CONTENT

[0005] The technical problem to be solved by the utility model is to provide a substrate glue laying device, which can assist the laying of substrate glue, make the laying of substrate glue even and flat, and further improve the accuracy of the experiment.

[0006] In order to realize the above technical problem, the utility model provides a substrate glue laying device, which comprises a placing piece, a laying assembly and a clamping assembly, a plurality of reaction cavities are formed on the placing piece, the laying assembly is put into or taken out of the reaction cavity through the clamping assembly.

[0007] The laying assembly comprises a pressing plate and a supporting piece, the pressing plate, the supporting piece and the reaction cavity jointly form a containing cavity capable of containing substrate glue, and a glue injection channel is formed on the pressing plate to fill the substrate glue into the containing cavity.

[0008] Further, the supporting piece is a supporting rod, a plurality of supporting rods are arranged on the working surface of the pressing plate facing the substrate glue, the lower end surface of each supporting rod is in contact with the lower surface of the reaction cavity, and the outer wall of each supporting rod is in contact with the inner wall of the reaction cavity.

[0009] Further, the support is a barrel structure, the barrel structure is arranged on the working surface of the pressing plate facing the matrix glue, the lower end surface of each barrel structure is in contact with the lower surface of the reaction cavity, the outer wall of the barrel structure is in contact with the inner wall of the reaction cavity, and the cavity in the barrel structure and the lower surface of the reaction cavity jointly form a containing cavity capable of containing the matrix glue.

[0010] Further, the outer edge of the one end of the glue injection channel away from the pressing plate extends outwardly with a first protruding structure, and the first protruding structure is capable of being clamped with the clamping end of the clamping assembly.

[0011] Further, the clamping assembly comprises a first clamping rod and a second clamping rod, the first clamping rod and the second clamping rod are hingedly connected with each other through a hinge point, the one end of the first clamping rod close to the first protruding structure and the one end of the second clamping rod close to the first protruding structure are respectively formed with the clamping end, the upper surface of the clamping end is in abutment with the lower surface of the first protruding structure, and a reset element is arranged between the one end of the first clamping rod away from the pressing plate and the one end of the second clamping rod away from the pressing plate, so that the first clamping rod and the second clamping rod can be spread apart.

[0012] Further, the working surface of the pressing plate, the support and the glue injection channel facing the matrix glue is formed with a protective layer structure, and the protective layer structure is a Teflon formed part.

[0013] Further, the cover plate assembly comprises a cover plate and a sealing part, the sealing part is arranged on the working surface of the cover plate facing the reaction cavity, the outer peripheral surface of the sealing part is capable of being in contact with the inner wall of the reaction cavity, and the working surface of the sealing part facing the laying assembly is capable of being in abutment with the end surface of the laying assembly facing the sealing part.

[0014] Further, a sealing part is arranged between the outer peripheral surface of the sealing part and the inner surface of the reaction cavity, and the sealing part is a rubber formed part.

[0015] Further, a reaction cup is further arranged, the reaction cup is arranged in the reaction cavity, the opening of the reaction cup faces away from the placing part, the reaction cup divides the reaction cavity into a first reaction cavity and a second reaction cavity from top to bottom, and the laying assembly is arranged in the first reaction cavity.

[0016] Further, the outer edge of the opening of the reaction cup extends in a direction away from the reaction cup with a second protruding structure, and the second protruding structure is located between the cover plate assembly and the placing part.

[0017] Through the above technical scheme, the beneficial effects of the present application are as follows:

[0018] The utility model provides a kind of matrix glue laying device, wherein, by setting laying assembly same with the laying of auxiliary matrix glue, to be able to the evenness of matrix glue laying, it is favorable to improve the accuracy of later experimental result, the matrix glue laying device provided by the utility model, simple structure, easy to operate.

[0019] Other features and advantages of the utility model will be described in detail in the subsequent specific implementation manner part. BRIEF DESCRIPTION OF DRAWINGS

[0020] The accompanying drawings are included to provide a further understanding of the utility model, and constitute part of the specification, and are used to explain the utility model together with the following specific implementation, but do not constitute the limitation to the utility model.In the drawings:

[0021] Figure 1 It is the structure schematic view of the specific implementation of the matrix glue laying device of the utility model;

[0022] Figure 2 It is the perspective view of one specific implementation of laying assembly in the matrix glue laying device of the utility model;

[0023] Figure 3 It is the front view of one specific implementation of laying assembly in the matrix glue laying device of the utility model;

[0024] Figure 4 It is Figure 3 Sectional view of A-A of

[0025] Figure 5 It is the perspective view of another specific implementation of laying assembly in the matrix glue laying device of the utility model;

[0026] Figure 6 It is the front view of another specific implementation of laying assembly in the matrix glue laying device of the utility model;

[0027] Figure 7 It is Figure 6 Sectional view of B-B of

[0028] Figure 8 It is the structure schematic view of the specific implementation of the clamping assembly in the matrix glue laying device of the utility model;

[0029] Figure 9 It is the structure schematic view of one specific implementation of the matrix glue laying device of the utility model and being provided with cover plate assembly;

[0030] Figure 10It is another specific embodiment structure diagram of the base matrix glue laying device provided with the cover plate assembly.

[0031] Explanation of reference signs

[0032] Specific embodiment

[0033] The specific embodiments described herein are intended to be illustrative only and are presented for the purpose of explanation and should not be construed as limiting the scope of the present application. It will be readily understood that the specific embodiments described herein are not limited to the specific embodiments described herein.

[0034] In the description of the present application, it should be pointed out that, unless otherwise explicitly specified and limited, the terms "provided", "provided with", "installed", "connected" should be understood broadly, for example, the connection can be direct connection, or indirect connection through intermediate medium, can be fixed connection, or detachable connection, or integral connection. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0035] In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features, therefore, the features limited by "first", "second" can explicitly or implicitly include one or more of the features.

[0036] In the present application, the orientation words such as "upper", "lower" are defined with the inside and outside of the corresponding components, in addition, "facing", "backing" are defined with the direction of the base matrix glue laying device, specifically in the drawings provided by the present application, the orientation or position relationship used is based on the orientation or position relationship shown in the drawings, the contact is only for the convenience of describing the present application and simplifying the description, and is not indicative or suggestive of the device or element indicated must have a specific orientation, be constructed and operated in a specific orientation, therefore, it cannot be understood as a limitation of the present application; for the orientation terms of the present application, it should be understood in combination with the actual installation state.

[0037] Reference Figures 1 to 10The utility model provides a kind of matrix glue laying device, including placing piece 1, laying assembly 2 and clamping assembly 3, and several reaction cavities 11 are formed on placing piece 1, laying assembly 2 is placed in reaction cavity 11 or is taken out from reaction cavity 11 by clamping assembly 3, and laying assembly 2 includes compression plate 21 and support 22, and the containment cavity 23 capable of accommodating matrix glue is formed by compression plate 21, support 22 and reaction cavity 11, and glue injection channel 24 is formed on compression plate 21, to be able to fill matrix glue inside containment cavity 23. Among them, placing piece 1 is as the corresponding culture plate required by experiment or is used to place the placing plate of Transwell chamber (permeable cell culture chamber), and several reaction cavities 11 are formed on placing piece 1, and reaction cavity 11 is as the reaction dish used in experiment;Laying assembly 2 is used to assist laying matrix glue, to be able to prevent the surface of injected matrix glue from being inclined or concave-convex, guarantee the volume and thickness of matrix glue, make the matrix glue that is laid thick and thin uniform, facilitate the error of control experiment;Clamping assembly 3 is used to clamp laying assembly 2, to be able to place laying assembly 2 in reaction cavity 11, or take out from reaction cavity 11.

[0038] It needs to be explained that laying assembly 2 includes compression plate 21 and support 22, wherein the containment cavity 23 capable of accommodating matrix glue is formed by compression plate 21, support 22 and reaction cavity 11, and glue injection channel 24 is formed on compression plate 21 to fill matrix glue into containment cavity 23. Containment cavity 23 is as the cavity capable of accommodating matrix glue, can limit the volume and thickness of matrix glue, matrix glue is injected into containment cavity 23 from glue injection channel 24, compression plate 21 is flattened by the thickness of itself to matrix glue, guarantee the flatness and laying uniformity of matrix glue, support 22 can guarantee the spacing between compression plate 21 and the bottom of reaction cavity 11, and guarantee that compression plate 21 and the bottom of reaction cavity 11 are mutually parallel, guarantee the uniformity and flatness of matrix glue laying.

[0039] It also needs to be explained that the method for using the matrix glue laying device provided by the utility model is as follows:

[0040] Firstly, select appropriate model of matrix glue laying device according to experimental type and cell culture plate size. According to the requirements of each experiment on the volume and thickness of matrix glue, the size of containment cavity 23 is set, and each model of matrix glue laying device corresponds to a specific amount of matrix glue when placing piece 1 is used.

[0041] Then, pre-place laying assembly 2 on placing piece 1 as culture plate by clamping assembly, that is, in the hole or Transwell chamber (permeable cell culture chamber) as reaction cavity 11, and pre-cool in refrigerator at 4 degrees.

[0042] Then, during the experiment, a certain amount of liquid matrix glue is injected into the containing cavity 23 from the glue injection channel 24 by using a pipette, so that the matrix glue fills the containing cavity 23;

[0043] Finally, after being placed at a temperature of 37° for 30 min, the auxiliary device is taken out by using the clamping assembly 3, at this time, the matrix glue is retained in the reaction cavity 11 of the hole / Transwell chamber (permeable cell culture chamber) as a culture plate for subsequent experiments.

[0044] The matrix glue laying device provided by the utility model has the advantages of simple structure, easy operation, auxiliary laying of matrix glue, ensured flatness and uniformity of matrix glue laying, controlled experimental error, and ensured precision of experimental results. In addition, the manufacturing material of the laying assembly 2 can be metal material, the metal material has a proper self-weight, helps to flatten the matrix glue, prevents the surface of the injected matrix glue from being inclined or concave-convex, and the metal material can be easily cooled and pre-cooled, prevents the matrix glue from being prematurely solidified, avoids problems of uneven laying of the matrix glue, and also avoids problems of blockage of the glue injection channel 24 or the pipette caused by premature solidification of the matrix glue.

[0045] Further, referring to Figures 2 to 4 , as a specific embodiment of the laying assembly 2 in the matrix glue auxiliary device of the utility model, the support 22 of the laying assembly 2 is a support rod, and the plurality of support rods are arranged on the working surface of the pressing plate 21 facing the matrix glue, the lower end surface of each support rod is in contact with the lower surface of the reaction cavity 11, and the outer wall of each support rod is in contact with the inner wall of the reaction cavity 11. The use of the support rod as the support 22 not only ensures the space between the pressing plate 21 and the bottom of the reaction cavity 11, so that the pressing plate 21 is horizontal relative to the bottom of the reaction cavity 11, but also does not occupy the space of the matrix glue.

[0046] In addition, referring to Figures 5 to 7 , as another specific embodiment of the support 22, the support 22 is a barrel-shaped structure, the barrel-shaped structure is arranged on the working surface of the pressing plate 21 facing the matrix glue, the lower end surface of each barrel-shaped structure is in contact with the lower surface of the reaction cavity 11, the outer wall of the barrel-shaped structure is in contact with the inner wall of the reaction cavity 11, and the cavity in the barrel-shaped structure and the lower surface of the reaction cavity 11 jointly form a containing cavity 23 capable of containing the matrix glue. The support 22 of the barrel-shaped structure cooperates with the pressing plate 21 to limit the use volume of the matrix glue, and the matrix glue can be uniformly laid in the upward and downward directions and the circumferential direction, and in some experiments in which a reaction space is required in the reaction cavity 11, a certain reaction space can also be provided.

[0047] Referring to Figures 1 to 10, as a specific embodiment of the utility model, the outer edge of one end of glue injection channel 24 away from the pressing plate 21 extends outwardly with the first protruding structure 25, the first protruding structure 25 can be connected with the clamping end 33 of the clamping assembly 3. The first protruding structure 25 can facilitate the clamping assembly 3 to clamp the laying assembly 2, since the experimental apparatus as reactor vessel size is not big, and, the texture is thin, the greater holding force will cause the clamped assembly or the related assembly to produce deformation, setting the first protruding structure 25 can provide the part of the laying assembly 2 for easy clamping, facilitate use.

[0048] Further, referring to Figure 8 , as a specific embodiment of the clamping assembly 3, the clamping assembly 3 includes the first clamping rod 31 and the second clamping rod 32, the first clamping rod 31 and the second clamping rod 32 are hinged to each other through the hinge point 4, the end of the first clamping rod 31 close to the first protruding structure 25 and the end of the second clamping rod 32 close to the first protruding structure 25 are respectively formed with the clamping end 33, the upper surface of the clamping end 33 abuts against the lower surface of the first protruding structure 25, the end of the first clamping rod 31 away from the pressing plate 21 and the end of the second clamping rod 32 away from the pressing plate 21 are provided with the reset element 34, so that the first clamping rod 31 and the second clamping rod 32 can be spread apart. Among them, the first clamping rod 31 and the second clamping rod 32 are to facilitate the use of experimental personnel and contact with the laying assembly 2, the clamping end 33 is to provide the structure that is adapted to the first protruding structure 25, which can facilitate contact with the first protruding structure 25, and the reset element 34 can adopt a spring, in the initial state, the reset element can be in a half-compressed half-released state, when in use, the experimental personnel compress the first clamping rod 31 and the second clamping rod 32 in opposite directions, which also compresses the spring, at this time, the end of the first clamping rod 31 and the second clamping rod 32 that needs to contact the laying assembly 2 releases space for clamping the laying assembly 2, after the clamping end 33 contacts the first protruding structure 25, the experimental personnel can release the clamping assembly 3, at this time, the spring continues to clamp the laying assembly 2 due to its resilience, and as Figure 8 indicated, the lower surface of the clamping end 33 can contact the upper surface of the pressing plate 21, on the one hand, the weight of the clamping assembly 3 can be used to further compress the laying assembly 2, on the other hand, it will not affect the glue injection.

[0049] Further, as a specific embodiment of the utility model, referring to Figure 2A protective layer structure 26 is formed on the working surface of the pressing plate 21, the support 22 and the glue injection channel 24 towards the matrix glue, and the protective layer structure 26 is a Teflon forming piece. Teflon has non-stick, corrosion resistance and moisture resistance, almost all substances do not stick to the Teflon coating film, and the matrix glue is also the same, so that the accuracy of the experiment and the stability of the experimental environment can be ensured, that is, a layer of Teflon coating film can be covered on the inner and outer surfaces of the entire laying assembly 2.

[0050] In addition, referring to Figure 9 and Figure 10 , as a specific embodiment of the utility model, the matrix glue laying device provided by the utility model further comprises a cover plate assembly 5, the cover plate assembly 5 comprises a cover plate 51 and a sealing piece 52, the sealing piece 52 is arranged on the working surface of the cover plate 51 towards the reaction cavity 11, and the outer peripheral surface of the sealing piece 52 can be in contact with the inner wall of the reaction cavity 11, and the working surface of the sealing piece 52 towards the laying assembly 2 can abut against the end surface of the laying assembly 2 towards the sealing piece 52. Among them, the sealing piece 52 can further press the laying assembly 2 on one hand, and on the other hand, it can be used as the sealing piece 52 of the reaction cavity 11, to prevent the reactions in adjacent reaction cavities 11 from interfering with each other, and the sealing piece 52 on the cover plate 51 is arranged corresponding to the reaction cavity 11 on the placing piece 1, to ensure that the reactions in each reaction cavity 11 are stably carried out, to ensure the experimental precision, and further fix the laying assembly 2, to avoid shaking of the device during movement by the experimenter, and to affect the matrix glue forming.

[0051] Further, a sealing piece 53 is arranged between the outer peripheral surface of the sealing piece 52 and the inner surface of the reaction cavity 11, and the sealing piece 53 is a rubber forming piece. In some experiments with high requirements for the environment in the reaction cavity 11, the sealing piece 53 can be arranged between the outer peripheral surface of the sealing piece 52 and the inner surface of the reaction cavity 11 to further ensure the sealing of the reaction cavity 11 and ensure the experimental precision, wherein a rubber forming piece with certain deformation capacity can be used.

[0052] In addition, when performing experiments such as organoid culture or cell invasion, the upper and lower parts of the test object need to have a certain reaction space, at this time, a Transwell (permeable cell culture) nest needs to be used, therefore, the laying assembly 2 provided by the utility model further comprises a reaction cup body 6, the reaction cup body 6 is built-in in the reaction cavity 11, and the reaction cup body 6 opens back to the placing piece 1, the reaction cup body 6 divides the reaction cavity 11 into a first reaction cavity 12 and a second reaction cavity 13 from top to bottom, and the laying assembly 2 is arranged in the first reaction cavity 12.

[0053] It should be noted that the bottom of the reaction vessel 6 is a porous membrane, and the reaction vessel 6 can divide the reaction chamber 11 into a first reaction chamber 12 and a second reaction chamber 13 to facilitate the experiment.

[0054] Specifically, such as Figure 10 As shown, a second protruding structure 61 extends from the outer edge of the opening of the reaction vessel 6 away from the reaction vessel 6. The second protruding structure 61 is located between the cover assembly 5 and the placement member 1. In order to ensure that the reaction vessel 6 can be placed stably in the reaction chamber 11, and that the reaction in the reaction chamber 11 will not be affected even when the experimenter moves it, the second protruding structure 61 is provided on the reaction vessel 6. The reaction vessel 6 is further fixed by the cooperation of the cover assembly 5 and the placement member 1.

[0055] The substrate adhesive spreading device provided by this invention can spread the substrate adhesive evenly and uniformly, ensuring a consistent thickness. The device has a simple structure, is easy to use, optimizes experimental procedures, and significantly reduces experimental errors.

[0056] In the description of this utility model, the reference to terms such as "one embodiment," "some embodiments," and "a specific implementation" indicates that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this utility model. In this utility model, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0057] The preferred embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0058] It should also be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, this utility model will not describe the various possible combinations separately.

[0059] Furthermore, various different embodiments of this utility model can be combined in any way, as long as they do not violate the spirit of this utility model, they should also be regarded as the content disclosed by this utility model.

Claims

1. A Matrigel® gel laying device comprising a placing member (1), a laying assembly (2) and a clamping assembly (3), characterized in that, A plurality of reaction cavities (11) are formed on the placing member (1), and the laying assembly (2) is put into or taken out of the reaction cavities (11) through the clamping assembly (3); The laying assembly (2) comprises a pressing plate (21) and a support member (22), the pressing plate (21), the support member (22) and the reaction cavities (11) jointly form a containing cavity (23) capable of containing matrix glue, and a glue injection channel (24) is formed on the pressing plate (21) to enable the containing cavity (23) to be filled with the matrix glue.

2. The Matrigel® gel laying device of claim 1, wherein, The support member (22) is a support rod, a plurality of support rods are arranged on the working surface of the pressing plate (21) facing the matrix glue, the lower end surface of each support rod is in contact with the lower surface of the reaction cavity (11), and the outer wall of each support rod is in contact with the inner wall of the reaction cavity (11).

3. The Matrigel® gel laying device of claim 1, wherein, The support member (22) is a barrel-shaped structure, the barrel-shaped structure is arranged on the working surface of the pressing plate (21) facing the matrix glue, the lower end surface of each barrel-shaped structure is in contact with the lower surface of the reaction cavity (11), the outer wall of the barrel-shaped structure is in contact with the inner wall of the reaction cavity (11), and the cavity in the barrel-shaped structure and the lower surface of the reaction cavity (11) jointly form a containing cavity (23) capable of containing the matrix glue.

4. The Matrigel® gel laying device of claim 1, wherein, The outer edge of the end of the glue injection channel (24) away from the pressing plate (21) extends outwardly to form a first protruding structure (25), and the first protruding structure (25) is capable of being clamped with the clamping end (33) of the clamping assembly (3).

5. The Matrigel® gel laying device of claim 4, wherein, The clamping assembly (3) comprises a first clamping rod (31) and a second clamping rod (32), the first clamping rod (31) and the second clamping rod (32) are hingedly connected to each other through a hinge point (4), the end of the first clamping rod (31) close to the first protruding structure (25) and the end of the second clamping rod (32) close to the first protruding structure (25) are respectively provided with the clamping end (33), the upper surface of the clamping end is in abutment with the lower surface of the first protruding structure (25), and a reset element (34) is arranged between the end of the first clamping rod (31) away from the pressing plate (21) and the end of the second clamping rod (32) away from the pressing plate (21) to enable the first clamping rod (31) and the second clamping rod (32) to be stretched apart.

6. The Matrigel® gel laying device according to any one of claims 1 to 5, wherein, The working surface of the pressing plate (21), the support member (22) and the glue injection channel (24) facing the matrix glue is provided with a protective layer structure (26), and the protective layer structure (26) is a Teflon shaped member.

7. The Matrigel® gel laying device of claim 6, wherein, The cover plate assembly (5) comprises a cover plate (51) and a sealing member (52) arranged on the working surface of the cover plate (51) facing the reaction cavity (11), and the outer peripheral surface of the sealing member (52) can be in contact with the inner wall of the reaction cavity (11), and the working surface of the sealing member (52) facing the laying assembly (2) can abut against the end surface of the laying assembly (2) facing the sealing member (52).

8. The Matrigel® gel laying device of claim 7, wherein, A sealing member (53) is arranged between the outer peripheral surface of the sealing member (52) and the inner surface of the reaction cavity (11), and the sealing member (53) is a rubber molded part.

9. The Matrigel® gel laying device of claim 8, wherein, The reaction cup body (6) is arranged in the reaction cavity (11), and the opening of the reaction cup body (6) faces away from the placing member (1), the reaction cup body (6) divides the reaction cavity (11) into a first reaction cavity (12) and a second reaction cavity (13) from top to bottom, and the laying assembly (2) is arranged in the first reaction cavity (12).

10. The Matrigel® gel laying device of claim 9, wherein, The opening outer edge of the reaction cup body (6) extends in a direction away from the reaction cup body (6) and has a second protruding structure (61), and the second protruding structure (61) is located between the cover plate assembly (5) and the placing member (1).

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