Flow cytometer microwell plate sample introduction mechanism

Abstract

The utility model relates to the technical field of cytometers, and discloses a flow cytometer microwell plate sample introduction mechanism which comprises a uniform mixing module and a driving module capable of driving the uniform mixing module to move along the X-axis direction and rotate around the Z-axis direction, the uniform mixing module comprises a middle connecting plate, one side of the middle connecting plate is provided with a bottom plate connected with the output end of the driving module, the other side of the middle connecting plate is provided with a top plate used for bearing a microwell plate, and the middle connecting plate is provided with a vortex driving assembly capable of driving the top plate to translate around the Z-axis direction; the vortex driving assembly drives the top plate to translate around the Z-axis direction relative to the middle connecting plate and the bottom plate, so that vortex mixing of samples in the microwell plate carried by the top plate can be realized, and the sample mixing effect is improved; and under the action of the driving module, the uniform mixing module can drive the microwell plate to move along the X-axis direction and rotate around the Z-axis direction, so that the sample injection mode is compact in structure, and the occupied space is greatly reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to cellometer technical field, concretely relates to a flow cytometer microporous plate sampling mechanism. BACKGROUND

[0002] The flow cytometer microporous plate sampling mechanism is mainly to accurately transport the sample in the microporous plate (such as 96 well plate or 384 well plate) to the detection area of flow cytometer, and usually in the sampling process, a sampling needle is used, which is accurately positioned to each hole position of the microporous plate, and when sampling is needed, the sampling needle is lowered to the appropriate depth in the hole, and then the sample is sucked into the pipeline system by suction.

[0003] At present, most of the flow cytometer microporous plate sampling mechanisms on the market adopt the XY axis translation sampling mode, and the structure of this sampling mode is large, which needs to occupy a large space, and this sampling mode uses suction and spitting mixing or sampling needle vibration mixing mode, which needs more complex host performance, but the mixing effect is not as good as vortex mixing. UTILITY MODEL CONTENT

[0004] The utility model discloses a flow cytometer microporous plate sampling mechanism, and the mixing module drives the microporous plate to realize vortex mixing, and the driving module drives the mixing module to move along the X axis direction and rotate around the Z axis direction, which can improve the mixing effect of the sample and reduce the space occupied by the sampling structure.

[0005] To achieve the above object, the utility model adopts the following technical scheme:

[0006] A flow cytometer microporous plate sampling mechanism, comprising a mixing module and a driving module capable of driving the mixing module to move along the X axis direction and rotate around the Z axis direction, wherein the mixing module comprises a middle connecting plate, one side of the middle connecting plate is provided with a bottom plate connected with the output end of the driving module, the other side is provided with a top plate for accommodating the microporous plate, and a vortex driving assembly capable of driving the top plate to move horizontally around the Z axis direction is arranged on the middle connecting plate.

[0007] Optionally, the vortex driving assembly comprises an eccentric shaft parallel to the Z axis direction, both ends of the eccentric shaft are connected with the top plate and the middle connecting plate respectively, and a driven wheel is mounted on the eccentric shaft.

[0008] A mixing motor is mounted on the middle connecting plate, a driving wheel is mounted on the output end of the mixing motor, and the driving wheel and the driven wheel are connected through a synchronous belt.

[0009] Optionally, the eccentric shaft is provided with three eccentric shafts, which are distributed in a triangular shape on the outside of the mixing motor.

[0010] Optionally, a driven wheel is mounted on each eccentric shaft, and a counterweight is fixedly connected to the driven wheel.

[0011] Optionally, the eccentric shaft comprises a shaft shoulder, one end of the shaft shoulder is connected with a first shaft body and a first shaft neck in sequence, and the other end is connected with a second shaft body and a second shaft neck in sequence; the first shaft neck is connected with the bottom plate, the second shaft neck is connected with the top plate, the driven wheel is sleeved on the first shaft body, and the first shaft body and the second shaft body are different in axis.

[0012] Optionally, the driving module comprises a base, a sliding plate capable of moving horizontally along the X-axis direction is arranged on the base, and a rotating disc capable of rotating around the Z-axis is arranged on the sliding plate.

[0013] The bottom plate is sleeved on the outside of the rotating disc, and a locking assembly for locking the bottom plate and the rotating disc is further arranged on the bottom plate.

[0014] Optionally, a support plate is fixedly mounted on the sliding plate, and a rotating shaft is arranged through the support plate; a rotating driving assembly for driving the rotating shaft to rotate is further mounted on the sliding plate, the output end of the rotating driving assembly is connected with the lower end of the rotating shaft, and the upper end of the rotating shaft is connected with the rotating disc.

[0015] Optionally, a sliding rail assembly parallel to the X-axis direction is mounted on the base, the sliding plate is slidably connected with the base through the sliding rail assembly, and an X-axis linear module is further mounted on the base, and the output end of the X-axis linear module is connected with the sliding plate.

[0016] Optionally, a sliding hole is formed in the side surface of the bottom plate, and a groove corresponding to the sliding hole is formed in the outer circumferential surface of the rotating disc.

[0017] The locking assembly comprises a locking block slidably arranged in the sliding hole, one end of the locking block is connected with a push plate, and the other end can be embedded in or separated from the groove along with the sliding of the push plate.

[0018] Optionally, a limiting sliding groove and a guide groove parallel to the X-axis direction are formed in the locking block, a limiting rod and a guide rod are fixedly arranged through the bottom plate, the lower part of the limiting rod is slidably embedded in the limiting sliding groove, and the lower end of the guide rod is slidably embedded in the guide groove.

[0019] Advantages

[0020] (1) The utility model discloses a mixing module, and the vortex drive assembly drives the top plate to move in the Z axis direction relative to the middle connecting plate and the bottom plate, so that the sample in the microwell plate received by the top plate can be mixed by vortex, and the mixing effect of the sample is improved. Under the action of the driving module, the mixing module can drive the microwell plate to move along the X axis direction and rotate around the Z axis direction. The structure of this sample feeding mode is compact, and the occupied space is greatly reduced.

[0021] (2) For the vortex drive assembly, the mixing motor drives the eccentric shaft to rotate through the transmission structure. Since the two ends of the eccentric shaft are connected with the top plate and the middle connecting plate respectively, when the eccentric shaft rotates, the top plate will move relative to the middle connecting plate, and the trajectory of the movement is a circle around the Z axis direction, thereby driving the sample on the top plate to vibrate and mix by vortex, improving the mixing effect of the sample.

[0022] (3) For the eccentric shaft, the synchronous belt is used to pass through the three eccentric shafts to conduct power transmission, which can increase the stress area and avoid movement jamming caused by uneven stress. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 is the structure schematic diagram of the flow cytometer microwell plate sample feeding mechanism of the utility model embodiment;

[0024] Figure 2 is the main view structure schematic diagram of the flow cytometer microwell plate sample feeding mechanism of the utility model embodiment;

[0025] Figure 3 is the structure schematic diagram of the vortex drive assembly in the utility model embodiment;

[0026] Figure 4 is the assembly structure schematic diagram of the eccentric shaft and the driven wheel in the utility model embodiment;

[0027] Figure 5 is the structure schematic diagram of the eccentric shaft in the utility model embodiment;

[0028] Figure 6 is the structure schematic diagram of the driving module in the utility model embodiment;

[0029] Figure 7 is the assembly structure schematic diagram of the bottom plate and the turntable in the utility model embodiment;

[0030] Figure 8 is the structure schematic diagram of the locking assembly in the utility model embodiment;

[0031] Among them, 1, mixing module;11, bottom plate;12, middle connecting plate;13, top plate;

[0032] 14. Scroll drive assembly; 141. Eccentric shaft; 1411. Shaft shoulder; 1412. First shaft body; 1413. First journal; 1414. Second shaft body; 1415. Second journal;

[0033] 142. Driven pulley; 143. Mixing motor; 144. Driving pulley; 145. Synchronous belt; 146. Counterweight; 15. Sliding hole;

[0034] 3. Drive module; 31. Base; 32. Slide plate; 33. Support plate; 34. Rotary shaft; 35. Rotary drive assembly; 36. Turntable; 361. Positioning groove; 362. Groove; 37. Slide rail assembly; 38. X-axis linear module;

[0035] 4. Locking assembly; 41. Push plate; 42. Locking block; 43. Limiting slide; 44. Guide groove; 45. Limiting rod; 46. Guide rod; 5. Lower pin; 6. Mixing control plate. Detailed Implementation

[0036] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams, which are only used to illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0037] Example 1

[0038] like Figure 1 As shown, a flow cytometer microplate sample introduction mechanism includes a mixing module 1 and a driving module 3. The former can drive the microplate to perform vortex mixing, and the latter can drive the microplate to move along the X-axis and rotate around the Z-axis. Compared with existing aspiration and expulsion mixing or sampling needle vibration mixing methods, vortex mixing is not only simple in structure and has low requirements for the performance of the host, but also has a better mixing effect. Compared with the XY axis translation sample introduction method, the X-axis translation and Z-axis rotation method adopted by this mechanism can reduce the space occupied and improve the space utilization efficiency.

[0039] like Figures 1-5 As shown, the mixing module 1 includes a bottom plate 11, a middle connecting plate 12, and a top plate 13 arranged sequentially from bottom to top. The three are parallel to each other, and the middle connecting plate 12 is provided with a vortex drive assembly 14 that can drive the top plate 13 to translate around the Z-axis. The bottom plate 11 is connected to the output end of the drive module 3, and the top plate 13 is used to support and hold microporous plates, such as 96-well plates, while test tubes are placed on the microporous plates.

[0040] The vortex drive assembly 14 can drive the top plate 13 to translate relative to the middle plate 12 and the bottom plate 11 around the Z-axis, so that the sample in the microplate supported by the top plate 13 can be vortex mixed, thereby improving the mixing effect of the sample.

[0041] The above-mentioned vortex driving assembly 14 comprises an eccentric shaft 141 parallel to the Z-axis direction, both ends of the eccentric shaft 141 are connected with the top plate 13 and the middle connecting plate 12 respectively, and a driven wheel 142 is installed on the eccentric shaft 141; a mixing motor 143 is installed on the middle connecting plate 12, an output end of the mixing motor 143 is provided with a driving wheel 144, and the driving wheel 144 and the driven wheel 142 are connected through a synchronous belt 145.

[0042] The bottom plate 11 and the middle connecting plate 12 are fixedly connected through a stand column, and the mixing motor 143 is provided with an installation and working space; the middle connecting plate 12 and the top plate 13 are connected through the eccentric shaft 141, and both ends of the eccentric shaft 141 are rotatably connected with the middle connecting plate 12 and the top plate 13.

[0043] The mixing motor 143 transmits torque to the eccentric shaft 141 through a belt transmission structure, that is, the mixing motor 143 first drives the driving wheel 144 to rotate, the driving wheel 144 drives the driven wheel 142 to rotate through the synchronous belt 145, and then the driven wheel 142 drives the eccentric shaft 141 to rotate, and finally, based on the eccentric structure of the eccentric shaft 141, the top plate 13 can rotate and translate relative to the middle connecting plate 12.

[0044] Regarding the rotation and translation of the top plate 13, when the eccentric shaft 141 rotates, the top plate 13 will translate relative to the middle connecting plate 12, and the trajectory of the translation is a circle around the Z-axis direction; and finally, the microplate is vibrated to realize the vortex mixing function.

[0045] The eccentric shaft 141 comprises a shaft shoulder 1411, one end of the shaft shoulder 1411 is sequentially connected with a first shaft body 1412 and a first shaft neck 1413, the other end is sequentially connected with a second shaft body 1414 and a second shaft neck 1415; the first shaft neck 1413 is rotatably connected with the bottom plate 11 through a bearing, the second shaft neck 1415 is rotatably connected with the top plate 13 through a bearing, the driven wheel 142 is cooperatively sleeved on the first shaft body 1412, and the first shaft body 1412 and the second shaft body 1414 are different shafts, that is, the axial directions of the two are parallel to each other and do not coincide, and the eccentric radius is 1.5 mm.

[0046] Further, the eccentric shaft 141 is provided with three, which are distributed in a triangular shape outside the mixing motor 143. The power transmission is conducted by the synchronous belt 145 around the three eccentric shafts 141, which can increase the stress area and avoid jamming caused by uneven stress. Each eccentric shaft 141 is provided with a driven wheel 142, and a counterweight 146 is fixedly connected to each driven wheel 142 to ensure the dynamic balance of the top plate 13 during movement.

[0047] Embodiment two

[0048] On the basis of the embodiment one, the utility model also proposes the specific structure of the driving module 3.

[0049] As Figures 1-2 , Figure 6 shown, the drive module 3 includes a base 31, the base 31 is provided with a slide plate 32 capable of moving horizontally along the X-axis direction, the slide plate 32 is provided with a turntable 36 capable of rotating around the Z-axis, and the bottom plate 11 is fitted on the outside of the turntable 36.

[0050] The bottom of the bottom plate 11 is provided with a mounting groove, and the bottom plate 11 can be fitted on the turntable 36 through the mounting groove. A positioning lug is arranged in the mounting groove, and a positioning groove 361 corresponding to the number and position of the positioning lug is arranged on the outer circumferential surface of the turntable 36; when the bottom plate 11 is fitted on the turntable 36, the positioning lug is embedded in the positioning groove 361, so that the turntable 36 can drive the entire mixing module 1 to rotate around the Z-axis direction through the bottom plate 11.

[0051] The slide plate 32 drives the turntable 36 to move horizontally along the X-axis direction on the base 31, and the turntable 36 drives the mixing module 1 to rotate around the Z-axis direction, thereby realizing the sample injection mode of X-axis translation and Z-axis rotation, reducing the occupied space of the overall structure, and reducing the demand for the performance of the main machine, thereby achieving the purpose of reducing the cost.

[0052] Among them, the top of the turntable 36 is provided with a lower pin needle 5, the top surface of the mounting groove is provided with an upper pin needle matched with the lower pin needle 5, and after the turntable 36 is embedded in the mounting groove of the bottom plate 11, the two groups of pin needles are mutually docked to realize electrical communication, so as to control the mixing module 1 through the mixing control panel 6.

[0053] The slide plate 32 is further provided with a slip ring circuit board between the slide plate 32 and the turntable 36. Through the slip ring circuit board 16 and the lower pin needle 5, the control signal can be introduced into the mixing control panel 6 of the mixing module 1, thereby realizing the control of the mixing work of the mixing module 1.

[0054] Regarding the rotary motion of the turntable 36 around the Z-axis, the slide plate 32 is fixedly installed on the slide plate 32 through a support rod, and a rotating shaft 34 is provided on the support plate 33; the slide plate 32 is further provided with a rotary drive assembly 35 for driving the rotating shaft 34 to rotate, the output end of the rotary drive assembly 35 is connected with the lower end of the rotating shaft 34, and the upper end of the rotating shaft 34 is connected with the turntable 36.

[0055] The rotary drive assembly 35 includes a motor, and the output end of the motor is connected with the lower end of the rotating shaft 34 through a belt transmission structure, that is, the output torque of the motor is transmitted to the rotating shaft 34 through the belt transmission structure, and then the rotating shaft 34 drives the turntable 36 to rotate around the Z-axis direction; the belt transmission structure is a prior art, which will not be described in detail here.

[0056] As to the translational movement of the rotating disc 36 along the X-axis direction, a slide rail assembly 37 parallel to the X-axis direction is installed on the base 31, and the slide plate 32 is slidably connected with the base 31 through the slide rail assembly 37, and an X-axis linear module 38 is also installed on the base 31, and the output end of the X-axis linear module 38 is connected with the slide plate 32.

[0057] The X-axis linear module 38 and the slide rail assembly 37 are both prior arts, the former can adopt an electric cylinder, a pneumatic cylinder or an oil cylinder and the like to output linear movement, and the latter includes a slide rail and a slide block in sliding cooperation, the slide rail is fixedly connected with the base 31, and the slide block is fixedly connected with the slide plate 32, that is, under the driving of the X-axis linear module 38, the slide plate 32 moves along the slide rail assembly 37, and then drives the supporting plate 33 and the rotating disc 36 to move horizontally along the X-axis direction.

[0058] Embodiment Three

[0059] On the basis of the embodiments one and two, in order to ensure that the bottom plate 11 can be locked with the rotating disc 36 after being sleeved on the rotating disc 36, a locking assembly 4 is further arranged on the bottom plate 11.

[0060] As shown in Figure 1 , Figure 3 , Figures 6-8 , a slide hole 15 is formed in the side surface of the bottom plate 11, and a groove 362 corresponding to the slide hole 15 is formed in the outer circumferential surface of the rotating disc 36; the locking assembly 4 includes a locking block 42 slidably arranged in the slide hole 15, one end of the locking block 42 is connected with a push plate 41, and the other end can be embedded in or separated from the groove 362 along with the sliding of the push plate 41.

[0061] The push plate 41 is located on the outer side of the bottom plate 11, and the end of the locking block 42 adopts a circular arc structure corresponding to the outer circumferential surface of the rotating disc 36, and horizontal pushing of the push plate 41 along the X-axis direction can realize the locking and separation between the bottom plate 11 and the rotating disc 36; if the push plate 41 is pushed to the left along the X-axis direction, the end of the locking block 42 is separated from the groove 362, and the bottom plate 11 can be taken out from the rotating disc 36 along the Z-axis direction; if the push plate 41 is pushed to the right along the X-axis direction, the end of the locking block 42 is embedded in the groove 362, and the locking between the bottom plate 11 and the rotating disc 36 is realized, which ensures the stable operation of the mechanism during the sampling process.

[0062] Further, a limiting slide groove 43 and a guide groove 44 parallel to the X-axis direction are formed in the locking block 42, and a limiting rod 45 and a guide rod 46 are fixedly arranged on the bottom plate 11, and the lower part of the limiting rod 45 is slidably embedded in the limiting slide groove 43, and the lower end of the guide rod 46 is slidably embedded in the guide groove 44.

[0063] The end of the limiting rod 45 is embedded in the limiting sliding groove 43, the push plate 41 and the lock block 42 can be avoided to be separated from the bottom plate 11 along the Y-axis direction, and the bottom of the guide rod 46 is provided with rotating ball, the ball is embedded in the guide groove 44, the moving direction of the push plate 41 can be ensured, and the friction between the push plate 41 and the lock block 42 can be reduced, so that the push plate 41 is more labor-saving and convenient to push.

[0064] On the basis of the first to third embodiments, the working process of using the device is as follows:

[0065] S1, install the mixing module 1 on the turntable 36;

[0066] S2, connect the automatic sample feeding device with the matched flow cytometer, initialize the turntable 36 after power on, and confirm that the parameter setting is completed after system self-checking, and the turntable 36 is in a test-ready state;

[0067] S3, the micro-hole plate with the sample is installed on the top plate 13 of the mixing module 1;

[0068] S4, the automatic sample feeding device automatically runs, and the X-axis linear module 38 in the driving module 3 drives the turntable 36 to reach the specified position through the sliding plate 32;

[0069] S5, the turntable 36 is driven to rotate by the rotary driving assembly 35, the micro-hole plate A1 hole position is located directly below the sampling needle of the flow cytometer, the mixing motor 143 rotates to drive the eccentric shaft 141 to rotate, and the micro-hole plate is vortex mixed, after the mixing is completed, the sampling needle moves downward to sample;

[0070] S6, after the A1 hole position test is completed, the turntable 36 continues to rotate to make the next hole position align with the sampling needle, and the above-mentioned actions are repeated until all the hole positions are tested, and the driving module 3 retreats to the initial position with the mixing module 1;

[0071] S7, the micro-hole plate with the test completed is taken down, the micro-hole plate to be tested is replaced, and the next round of test is carried out.

[0072] If the mixing module 1 is replaced by a test tube disc, 5ml flow tubes can be compatible, and the operation mode is the same as above.

[0073] In summary, the flow cytometer micro-hole plate sample feeding mechanism provided by the utility model can realize 800-1500 rotation eccentric mixing, has good mixing effect, high motion precision and good repeatability, can be compatible with different models of 96-hole plates and 5ml flow tubes, has good applicability, the whole turntable 36 is automatically operated by the cytometer host, is convenient to use, has small noise by increasing the counterweight and shock absorption design, the operator puts the test tube disc into the instrument, clicks to start collection, and does not need subsequent manual intervention, the mechanical operation is stable, the common flow tubes and 96 shallow-hole plates (round bottom, V bottom and flat bottom) on the market can be compatible, and consumables are easy to obtain.

[0074] In the description of the utility model, it needs to understand that the orientation or position relation indicated by the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like is the orientation or position relation based on the drawings shown, and is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the utility model. In addition, the terms "first", "second" and the like are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features limited by "first", "second" and the like can explicitly or implicitly include one or more features. In the description of the utility model, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0075] In the description of the utility model, it needs to be explained that, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connection" should be understood broadly, for example, it can be fixed connection, can also be detachable connection, or integrally connected, can be mechanical connection, can also be electrical connection, can be directly connected, can also be indirectly connected through an intermediate medium, can be the communication inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood through specific circumstances.

[0076] According to the ideal embodiment of the utility model, through the above description, relevant personnel can make various changes and modifications without deviating from the technical idea of the utility model. The technical scope of the utility model is not limited to the contents in the specification, and the technical scope must be determined according to the scope of claims.

Claims

1. A flow cytometer microplate sample injection mechanism characterized by: The utility model provides a mixing module (1) and drive module (3) can drive mixing module (1) moves along X axle direction and rotates around Z axle direction, mixing module (1) includes the middle joint board (12), one side of middle joint board (12) is provided with the bottom plate (11) connected with the output end of drive module (3), the other side is provided with the top plate (13) for receiving micro -hole board, and the middle joint board (12) is provided with the vortex drive assembly (14) that can drive top plate (13) moves around Z axle direction.

2. The flow cytometer microwell plate sample injection mechanism of claim 1, wherein: The vortex drive assembly (14) includes an eccentric shaft (141) parallel to the Z-axis direction, both ends of the eccentric shaft (141) are connected with the top plate (13) and the middle joint board (12) respectively, and a driven wheel (142) is installed on the eccentric shaft (141). The middle joint board (12) is provided with a mixing motor (143), a driving wheel (144) is installed on the output end of the mixing motor (143), and the driving wheel (144) and the driven wheel (142) are connected through a synchronous belt (145).

3. The flow cytometer microwell plate sample injection mechanism of claim 2, wherein: The eccentric shaft (141) is provided with three, which are distributed on the outside of the mixing motor (143) in a triangular shape.

4. The flow cytometer microplate injection mechanism of claim 3, wherein: Each eccentric shaft (141) is provided with a driven wheel (142), and the driven wheel (142) is fixedly connected with a counterweight (146).

5. The flow cytometer microplate sample injection mechanism of claim 2, wherein: The eccentric shaft (141) includes a shaft shoulder (1411), one end of the shaft shoulder (1411) is sequentially connected with a first shaft body (1412) and a first shaft neck (1413), and the other end is sequentially connected with a second shaft body (1414) and a second shaft neck (1415). The first shaft neck (1413) is connected with the bottom plate (11), the second shaft neck (1415) is connected with the top plate (13), the driven wheel (142) is sleeved on the first shaft body (1412), and the first shaft body (1412) and the second shaft body (1414) are different shafts.

6. The flow cytometer microwell plate sample injection mechanism of claim 1, wherein: The drive module (3) includes a base (31), the base (31) is provided with a sliding plate (32) capable of moving horizontally along the X-axis direction, and the sliding plate (32) is provided with a rotating disc (36) capable of rotating around the Z-axis. The bottom plate (11) is sleeved on the outside of the rotating disc (36), and the bottom plate (11) is further provided with a locking assembly (4) for locking the bottom plate (11) and the rotating disc (36).

7. The flow cytometer microwell plate sample injection mechanism of claim 6, wherein: The sliding plate (32) is fixedly provided with a support plate (33), and a rotating shaft (34) penetrates through the support plate (33); the sliding plate (32) is further provided with a rotating drive assembly (35) for driving the rotating shaft (34) to rotate, the output end of the rotating drive assembly (35) is connected with the lower end of the rotating shaft (34), and the upper end of the rotating shaft (34) is connected with the rotating disc (36).

8. The flow cytometer microplate sample injection mechanism of claim 6, wherein: The base (31) is provided with a slide rail assembly (37) parallel to the X-axis direction, the slide plate (32) is slidably connected with the base (31) through the slide rail assembly (37), and the base (31) is further provided with an X-axis linear module (38), and an output end of the X-axis linear module (38) is connected with the slide plate (32).

9. The flow cytometer microplate sample injection mechanism of claim 6, wherein: The side surface of the bottom plate (11) is provided with a slide hole (15), and the outer circumferential surface of the rotating disc (36) is provided with a groove (362) corresponding to the slide hole (15). The locking assembly (4) comprises a locking block (42) slidably arranged in the slide hole (15), one end of the locking block (42) is connected with a push plate (41), and the other end can be embedded in or separated from the groove (362) by sliding the push plate (41).

10. The flow cytometer microwell plate sample injection mechanism of claim 9, wherein: The locking block (42) is provided with a limiting sliding groove (43) and a guide groove (44) parallel to the X-axis direction, the bottom plate (11) is fixedly provided with a limiting rod (45) and a guide rod (46), and the lower part of the limiting rod (45) is slidably embedded in the limiting sliding groove (43), and the lower end of the guide rod (46) is slidably embedded in the guide groove (44).

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