3D culture device for hydrogel-coated trace cells

Abstract

The utility model relates to a hydrogel coated trace cell 3D culture device, which comprises a coating device, the center of the coating device is provided with a sample adding hole, the height of the sample adding hole is 2-3mm, the volume of the sample adding hole is 10-200 [mu] l, the coating device with the sample adding hole as the circle center is provided with a liquid storage tank, the liquid storage tank is of an annular structure, and the liquid storage tank is connected with the coating device. The coating device is provided with a plurality of micro-channels, one end of each micro-channel is communicated with the sample adding hole, the other end of each micro-channel is communicated with the liquid storage tank, an umbrella-shaped structure is formed between the plurality of micro-channels and the sample adding hole, the width of each micro-channel is 50-55 microns, the height of each micro-channel is 50-200 microns, the length of each micro-channel is 0.5-2 mm, the volume of the liquid storage tank is 100-500 [mu] l, and the height of each liquid storage tank is 3-5 mm. According to the micro cell culture device, micro samples can be coated, and the culture efficiency of the micro cell samples is improved.

Description

Technical Field

[0001] This invention belongs to the field of biomedical engineering, and in particular relates to a hydrogel-coated micro-cell 3D culture device. Background Technology

[0002] With the development of cell biology and organoid technology, three-dimensional cell culture technology is gradually replacing traditional two-dimensional cell culture technology. Currently, many cell types possess strong self-assembly capabilities, such as pluripotent stem cells, tumor cells, and tissue cells. Three-dimensional cell spheres are three-dimensional aggregates formed by the self-assembly of various cells, more closely resembling the structural morphology of in vivo tissue cells and more conducive to the study of their functional mechanisms. Therefore, three-dimensional cell spheres can be used in numerous biological and biomedical research fields, such as developmental biology, pathology, pharmacology, and cancer treatment.

[0003] A search revealed a patent: a stem cell culture device for breast cancer immunotherapy (CN202410845158.2), which includes a culture chamber and a door. The culture chamber contains a support plate on which culture dishes are placed. The chamber also includes a fixing assembly and a medium-changing assembly. A control panel is located on the top of the culture chamber. Each culture dish contains at least two partitioning components, each including a support cylinder, a filter screen, a clamping plate, and partition blocks. The support cylinder is positioned from bottom to top within the culture dish. The filter screen is located at the bottom of the support cylinder, the clamping plate at the top, and multiple partition blocks are evenly distributed at the bottom of the support cylinder. This culture method is cumbersome and inefficient.

[0004] In view of the above-mentioned shortcomings, the designer has actively researched and innovated in order to create a novel hydrogel-coated micro-cell 3D culture device, making it more valuable for industrial applications. Utility Model Content

[0005] To solve the above-mentioned technical problems, the purpose of this utility model is to provide a hydrogel-coated micro-cell 3D culture device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A hydrogel-coated microcellular 3D culture device includes a coating device with a sample loading well at its center. The height of the sample loading well is 2 mm to 3 mm, and the volume of the sample loading well is 10 to 200 μl. A reservoir is formed on the coating device with the sample loading well as its center. The reservoir has a ring structure. Several microchannels are formed on the coating device. One end of each microchannel is connected to the sample loading well, and the other end of each microchannel is connected to the reservoir. The microchannels and the sample loading well form an umbrella-like structure. The width of each microchannel is 50 μm to 55 μm, the height is 50 to 200 μm, and the length is 0.5 to 2 mm. The volume of the reservoir is 100 to 500 μl, and the height is 3 to 5 mm.

[0008] Preferably, in the hydrogel-coated micro-cell 3D culture device, the volume of the sample well is 10 μl and the height of the sample well is 2 mm.

[0009] Preferably, in the hydrogel-coated microcell 3D culture device, the number of microchannels is 16, the width of the microchannel is 50μm, the height is 50μm, and the length is 2mm.

[0010] Preferably, in the hydrogel-coated micro-cell 3D culture device, the volume of the storage tank is 500 μL and the height is 5 mm.

[0011] Preferably, in the hydrogel-coated microcell 3D culture device, the volume of the sample well is 50 μl and the height of the sample well is 3 mm.

[0012] Preferably, in the hydrogel-coated microcell 3D culture device, the number of microchannels is 8, the width of the microchannel is 50μm, the height is 50μm, and the length is 1mm.

[0013] Preferably, in the hydrogel-coated micro-cell 3D culture device, the volume of the reservoir is 300 μL and the height is 3 mm.

[0014] Preferably, in the hydrogel-coated microcell 3D culture device, the coating device is prepared by injection molding, and the microchannels and the liquid reservoir are bonded together by one of the following connection methods: hot pressing, laser welding or ultrasonic welding.

[0015] By means of the above solution, this utility model has at least the following advantages:

[0016] This invention can coat trace samples, improving the culture efficiency of trace cell samples. Furthermore, it requires no complex equipment and is simple and convenient to operate.

[0017] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a top view of the present invention. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0023] Example

[0024] like Figure 1 and Figure 2As shown, a hydrogel-coated microcellular 3D culture device includes a coating device 1. A sample loading well 2 is located at the center of the coating device 1. The height of the sample loading well 2 is 2 mm to 3 mm, and the volume of the sample loading well 2 is 10 to 200 μl. A reservoir 4 is formed on the coating device 1 with the sample loading well 2 as the center. The reservoir 4 has a ring-shaped structure. Several microchannels 3 are formed on the coating device 1. One end of each microchannel 3 is connected to the sample loading well 2, and the other end of each microchannel 3 is connected to the reservoir 4. The microchannels 3 and the sample loading well 2 form an umbrella-like structure. The width of each microchannel 3 is 50 μm to 55 μm, the height is 50-200 μm, and the length is 0.5-2 mm. The volume of the reservoir 4 is 100-500 μl, and the height is 3-5 mm.

[0025] The coating device 1 described in this invention is prepared by injection molding. The injection molding polymer material includes, but is not limited to, PS, PMMA, PC, etc. The bonding method of the device can be, for example, hot-press bonding, laser bonding, ultrasonic bonding, or other bonding methods known in the art.

[0026] The working principle of this utility model is as follows:

[0027] (1) Select a cell-coated hydrogel precursor and prepare a mixed solution with a trace cell sample;

[0028] (2) Select a suitable size of covering device. If necessary, the covering device can be placed on an ice bath.

[0029] (3) Add the droplet oil into the annular storage tank inside the coating device;

[0030] (4) Add the prepared cell sample and hydrogel precursor mixture into the sample well;

[0031] (5) Select a suitable positive pressure source 5 and apply it to the sample well to drive the mixed solution into the umbrella-shaped microchannel 3 and into the reservoir 4. Under the action of step emulsification, the mixed solution forms microdroplets loaded with cell samples. After the hydrogel is solidified, the hydrogel microspheres loaded with cell samples 6 are obtained.

[0032] The hydrogel precursors include, but are not limited to: Matrigel, collagen, alginate, GelMA, etc., with a hydrogel concentration of 0.1-20% and a cell suspension density of 10. 3 -10 8 pcs / ml; droplet oils include, but are not limited to: fluorocarbon oils, alkane oils, mineral oils, vegetable oils, and commercially available droplet oils;

[0033] Example 1

[0034] Based on the embodiments, the volume of the sample well 2 in this invention is 10 μl, the height of the sample well 2 is 2 mm, the number of microchannels 3 is 16, the width of the microchannels is 50 μm, the height is 50 μm, and the length is 2 mm, the volume of the reservoir 4 is 500 μl, the height is 5 mm, the hydrogel precursor is Matrigel, and the density of the cell suspension is 10. 4 pcs / ml; the droplet oil is a commercially available droplet oil.

[0035] Example 2

[0036] Based on the embodiments, the volume of the sample well 2 in this invention is 50 μl, and the height of the sample well 2 is 3 mm. The number of microchannels 3 is 8, with a width of 50 μm, a height of 50 μm, and a length of 1 mm. The volume of the reservoir 4 is 300 μl, and the height is 3 mm. The hydrogel precursor is Matrigel.

[0037] The density of the cell suspension is: 10 3 The droplet oil is commercially available; the coating device 1 is prepared by injection molding using a mold, and the injection molding polymer material is PS and is bonded by hot pressing.

[0038] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0039] In the description of this application, it should be noted that the terms "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0040] Furthermore, terms such as "horizontal" and "vertical" do not imply that components must be absolutely horizontal or vertical, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0041] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0042] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A hydrogel-coated micro-cell 3D culture device, characterized in that: The device includes a coating device (1), with a sample application hole (2) at its center. The height of the sample application hole (2) is 2 mm to 3 mm, and the volume of the sample application hole (2) is 10 to 200 μl. A liquid storage tank (4) is formed on the coating device (1) with the sample application hole (2) as the center. The liquid storage tank (4) has an annular structure. Several microchannels (3) are formed on the coating device (1). One end of each microchannel (3) is connected to the sample application hole (2), and the other end of each microchannel (3) is connected to the liquid storage tank (4). The microchannels (3) and the sample application hole (2) form an umbrella-shaped structure. The width of each microchannel (3) is 50 μm to 55 μm, the height is 50 to 200 μm, and the length is 0.5 to 2 mm. The volume of the liquid storage tank (4) is 100 to 500 μl, and the height is 3 to 5 mm.

2. The hydrogel-coated micro-cell 3D culture device according to claim 1, characterized in that: The volume of the sample loading well (2) is 10 μl, and the height of the sample loading well (2) is 2 mm.

3. The hydrogel-coated micro-cell 3D culture device according to claim 1, characterized in that: The number of microchannels (3) is 16, the width of the microchannel is 50μm, the height is 50μm, and the length is 2mm.

4. The hydrogel-coated micro-cell 3D culture device according to claim 1, characterized in that: The liquid storage tank (4) has a volume of 500 μL and a height of 5 mm.

5. The hydrogel-coated micro-cell 3D culture device according to claim 1, characterized in that: The volume of the sample loading well (2) is 50 μl, and the height of the sample loading well (2) is 3 mm.

6. The hydrogel-coated micro-cell 3D culture device according to claim 1, characterized in that: The number of microchannels (3) is 8, the width of the microchannel is 50μm, the height is 50μm, and the length is 1mm.

7. The hydrogel-coated micro-cell 3D culture device according to claim 1, characterized in that: The liquid storage tank (4) has a volume of 300 μL and a height of 3 mm.

8. The hydrogel-coated micro-cell 3D culture device according to claim 1, characterized in that: The coating device (1) is prepared by injection molding, and the microchannel and the liquid storage pool are bonded together by one of the following connection methods: hot pressing, laser welding or ultrasonic welding.

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