Ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card

Abstract

The utility model discloses a fluorescent quantitative rapid detection card for ofloxacin quantum dot microspheres. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card comprises a test strip, the test strip comprises a bottom plate, a sample pad, a fluorescent microsphere conjugate pad, a nitrocellulose membrane and a water absorption pad, the sample pad, the fluorescent microsphere conjugate pad, the nitrocellulose membrane and the water absorption pad are arranged on the bottom plate and connected in sequence, and detection lines and quality control lines which are distributed at intervals are arranged on the nitrocellulose membrane. The detection line is close to the fluorescent microsphere conjugate pad, the quality control line is close to the water absorption pad, the detection line is coated with a micromolecule ofloxacin antigen, the quality control line is coated with a goat anti-mouse IgG antibody, and a microsphere labeled antibody conjugate in the fluorescent microsphere conjugate pad is a fluorescent microsphere labeled ofloxacin antibody. The method is simple and rapid to operate, is less affected by environment temperature and solvent reagents, is suitable for rapid detection of food safety, and is accurate in detection result.

Description

Technical Field

[0001] This utility model relates to the field of food safety testing technology, and in particular to an ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card. Background Technology

[0002] Ofloxacin is an organic compound with the chemical formula C. 18 H 20 FN3O4 is a synthetic, broad-spectrum antibacterial fluoroquinolone drug, primarily used for acute and chronic infections of the respiratory tract, pharynx, tonsils, urinary tract (including the prostate), skin and soft tissues, gallbladder and bile ducts, middle ear, sinuses, dacryocystorhinostomy sac, and intestines caused by Gram-negative bacteria. Ofloxacin is a third-generation quinolone antibiotic with good antibacterial activity against Staphylococcus, Streptococcus (including Enterococcus), Streptococcus pneumoniae, Neisseria gonorrhoeae, Escherichia coli, Citrobacter, Shigella, Klebsiella pneumoniae, Enterobacter spp., Serratia spp., Proteus, Haemophilus influenzae, Acinetobacter, and Helicobacter pylori, and also has some antibacterial activity against Pseudomonas aeruginosa and Chlamydia trachomatis. Due to its broad-spectrum antibacterial capabilities, it is widely used in animal husbandry and clinical medicine. However, the extensive use of ofloxacin in livestock farming can lead to residues that can enter the human body through the food chain. These residues can cause kidney dysfunction (e.g., elevated BUN, elevated serum creatinine), elevated liver enzymes, decreased blood cells and platelets, gastrointestinal dysfunction, allergic reactions, and central nervous system symptoms (insomnia, dizziness, etc.). Furthermore, it can cause cancer, birth defects, and mutations. The national standard GB31650.1-2022 specifies different limits for ofloxacin, with a minimum residue limit of 2 μg / kg and a maximum residue limit of 5 μg / kg.

[0003] Currently, traditional methods for detecting ofloxacin mainly include colloidal gold assays and enzyme-linked immunosorbent assays (ELISA). These methods can accurately detect ofloxacin qualitatively and quantitatively, but colloidal gold assays cannot provide quantitative results. ELISA, on the other hand, has a long detection cycle, is complex to operate, and is greatly affected by ambient temperature, requiring laboratory testing, making it difficult to rapidly and effectively quantify ofloxacin in food. For example, quinolone detection cards and quinolone detection cards utilize colloidal gold immunochromatography, but neither can achieve quantitative results due to low sensitivity and poor specificity. Additionally, traditional techniques include ratiometric fluorescent probes: a commonly used fluorescent probe that uses the ratio of fluorescence intensity of a fluorescent dye at two different wavelengths to reflect the concentration or activity of a target substance. These probes mainly come in two forms: standalone ratiometric probes and paired complementary probes. Ratiometric fluorescent probe technology is greatly affected by external factors, such as light intensity and detector performance, requiring high spectral resolution and signal-to-noise ratio, and has relatively low sensitivity. Utility Model Content

[0004] Therefore, it is necessary to provide a rapid quantitative detection card for ofloxacin using quantum dot microspheres. This novel rapid quantitative detection card for ofloxacin using quantum dot microspheres is simple and fast to operate, less affected by ambient temperature and solvent reagents, suitable for rapid food safety testing, and provides accurate results.

[0005] One embodiment of this application provides a rapid quantitative detection card for ofloxacin using quantum dot microspheres.

[0006] A rapid quantitative detection card for ofloxacin using quantum dot microspheres includes a test strip. The test strip includes a base plate and a sample pad, a fluorescent microsphere conjugate pad, a nitrocellulose membrane, and an absorbent pad, which are sequentially connected on the base plate. The nitrocellulose membrane has spaced detection lines and control lines. The detection lines are closer to the fluorescent microsphere conjugate pad, and the control lines are closer to the absorbent pad. The detection lines are coated with small molecule ofloxacin antigen, and the control lines are coated with goat anti-mouse IgG antibody. The microsphere-labeled antibody conjugate in the fluorescent microsphere conjugate pad is a fluorescent microsphere-labeled ofloxacin antibody.

[0007] In some embodiments, the test strip has an elongated structure, and the sample pad, the fluorescent microsphere conjugate pad, the nitrocellulose membrane, and the absorbent pad all extend along the length of the test strip.

[0008] In some embodiments, the length of the test strip is 6cm to 8cm;

[0009] And / or, the width of the test strip is 2.5mm to 4mm.

[0010] In some embodiments, the fluoxetine quantum dot microsphere fluorescence quantitative rapid detection card further includes a housing, on which a detection window and a sample application hole are provided. The test strip is assembled inside the housing, with the detection line and the control line on the test strip located at the detection window position, and the sample pad located at the sample application hole position.

[0011] In some embodiments, the size of the sample application orifice gradually decreases from one side located on the outer surface of the housing to the side located inside the housing.

[0012] In some embodiments, the detection window is elongated, and the detection line and the control line are located in the middle of the detection window.

[0013] In some embodiments, the housing includes a cover and a base, the cover and the base being detachably connected, the test strip being assembled on the base, and the cover having a detection window and a sample application hole.

[0014] In some embodiments, the base is provided with a slot, and the test strip is inserted into the slot.

[0015] In some embodiments, the base and the cover are respectively provided with a limiting groove and a buckle, and the base and the cover are detachably connected through the limiting groove and the buckle.

[0016] In some embodiments, the housing has an identification area, which is provided with a one-dimensional barcode and / or a two-dimensional barcode that can be scanned and identified by an external scanning device.

[0017] And / or, at least one clearance groove for positioning the housing is also provided on the outer wall of the housing. The above-mentioned ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card, compared with quinolone detection cards and ratiometric fluorescent probe technology, is simple and rapid to operate, less affected by ambient temperature and solvent reagents, suitable for rapid food safety detection, and provides accurate results. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] To gain a more complete understanding of this application and its beneficial effects, the following description will be provided in conjunction with the accompanying drawings. In the following description, the same reference numerals denote the same parts.

[0020] Figure 1 This is a schematic diagram of an embodiment of the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card.

[0021] Figure 2 This is a schematic diagram of the test strip of the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to an embodiment of the present invention;

[0022] Figure 3 This is a schematic diagram of the base of the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card according to an embodiment of this utility model.

[0023] Explanation of reference numerals in the attached figures

[0024] 10. Ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card; 100. Test strip; 101. Base plate; 102. Sample pad; 103. Fluorescent microsphere coupling pad; 104. Nitrocellulose membrane; 105. Absorbent pad; 106. Detection line; 107. Quality control line; 201. Shell cover; 2011. Detection window; 2012. Sample dispensing hole; 202. Base; 203. Slot; 204. Limiting slot; 205. Buckle; 206. Relief slot. Detailed Implementation

[0025] To make the above-mentioned objects, features, and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a full understanding of this utility model. However, this utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed below.

[0026] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0027] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0028] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0029] In the description of this utility model, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0031] In this application, when numerical intervals (i.e., numerical ranges) are involved, unless otherwise specified, the distribution of selectable numerical values ​​within the numerical interval is considered continuous, and includes the two endpoints of the numerical interval (i.e., the minimum and maximum values), as well as every numerical value between these two endpoints. Unless otherwise specified, when a numerical interval refers only to integers within that numerical interval, it includes the two endpoint integers of the numerical range, as well as every integer between the two endpoints, which is equivalent to directly listing every integer. When multiple numerical ranges are provided to describe features or characteristics, these numerical ranges can be merged. In other words, unless otherwise specified, the numerical ranges disclosed in this application should be understood to include any and all subranges included therein. The "numerical value" in the numerical interval can be any quantitative value, such as a number, percentage, ratio, etc. The term "numerical interval" can be broadly included to include percentage intervals, ratio intervals, proportion intervals, etc.

[0032] This application provides a rapid quantitative detection card for ofloxacin using quantum dot microspheres, addressing the shortcomings of existing technologies such as low sensitivity, poor repeatability, and inability to rapidly detect and quantify ofloxacin. The rapid quantitative detection card for ofloxacin using quantum dot microspheres will be described below with reference to the accompanying drawings.

[0033] The ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 provided in this application embodiment is exemplary; please refer to [link to example]. Figure 1 As shown, Figure 1 This is a schematic diagram of the structure of the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10 provided in this application embodiment. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10 of this application can be used to detect ofloxacin residues in food, utilizing the high sensitivity, good stability, high recovery rate, and convenience of quantum dot fluorescent microspheres.

[0034] To more clearly illustrate the structure of the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10, the following description of the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10 will be provided in conjunction with the accompanying drawings.

[0035] For example, please refer to Figure 1 As shown, an ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10 includes a test strip 100. The test strip 100 includes a base plate 101 and a sample pad 102, a fluorescent microsphere conjugate pad 103, a nitrocellulose membrane 104, and an absorbent pad 105, which are disposed on the base plate 101 and connected in sequence. The nitrocellulose membrane 104 has detection lines 106 and control lines 107 spaced apart. The detection lines 106 are close to the fluorescent microsphere conjugate pad 103, and the control lines 107 are close to the absorbent pad 105. The detection lines 106 are coated with small molecule ofloxacin antigen, and the control lines 107 are coated with goat anti-mouse IgG antibody. The microsphere-labeled antibody conjugate in the fluorescent microsphere conjugate pad 103 is a fluorescent microsphere-labeled ofloxacin antibody.

[0036] It should be noted that the small molecule ofloxacin antigen in this application is formed by assembling luminescent quantum dots with nano- to micron-sized microspheres with functional groups on their surface, and then binding the antigen to them. In the field of quantitative biological detection, due to the complex and variable detection environment, biolabeling and detection place high demands on the stability of quantum dots, the sensitivity of detection, and the accuracy of the results. Quantum dot microspheres (QD nanobeads or QD nanospheres), as a type of fluorescent microsphere, assemble luminescent quantum dots with nano- to micron-sized microspheres with functional groups on their surface, thereby protecting the quantum dots, making the surface easy to modify, and amplifying the signal. This effectively compensates for the problems of insufficient signal intensity and poor repeatability of single quantum dots during detection. Therefore, quantum dot microspheres are widely used in labeling, food safety detection, tracing, and immunomedicine. Quantum dot microspheres are fluorescent microspheres prepared from multiple quantum dots. As fluorescent probes for detecting target substances, they have high sensitivity and advantages such as high stability, strong fluorescence intensity, and large specific surface area.

[0037] As can be seen, the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10, compared with quinolone detection cards and ratiometric fluorescent probe technology, is simple and fast to operate, less affected by ambient temperature and solvent reagents, and is suitable for rapid food safety detection with accurate results.

[0038] In some of these embodiments, see Figure 2 As shown, the test strip 100 has a long strip structure. The sample pad 102, the fluorescent microsphere conjugate pad 103, the nitrocellulose membrane 104, and the absorbent pad 105 all extend along the length of the test strip 100.

[0039] In some embodiments, the length of the test strip 100 is 6cm to 8cm. For example, the length of the test strip 100 described above is 6cm, 7cm, 8cm, or other values.

[0040] In some embodiments, the width of the test strip 100 is 2.5 mm to 4 mm. For example, the width of the test strip 100 described above is 2.5 mm, 2.8 mm, 3 mm, 3.5 mm, 4 mm, or other values.

[0041] In some embodiments, the fluoxetine quantum dot microsphere fluorescence quantitative rapid detection card also includes a housing. The housing has a detection window 2011 and a sample application port 2012. A test strip 100 is assembled inside the housing. The detection line 106 and control line 107 on the test strip 100 are located at the detection window 2011. A sample pad 102 is located at the sample application port 2012.

[0042] In some embodiments, the size of the sample dispensing orifice 2012 gradually decreases from one side located on the outer surface of the housing to the other side located inside the housing. The gradually decreasing size of the sample dispensing orifice 2012 facilitates sample dispensing and allows the sample solution to smoothly enter the sample pad 102.

[0043] In some embodiments, the detection window 2011 is elongated. The detection line 106 and the control line 107 are located in the middle of the detection window 2011.

[0044] In some of these embodiments, see Figure 3 As shown, the housing includes a cover 201 and a base 202. The cover 201 and the base 202 are detachably connected. The test strip 100 is assembled on the base 202. The cover 201 is provided with a detection window 2011 and a sample application hole 2012.

[0045] In some embodiments, the base 202 is provided with a slot 203. The test strip 100 is held in the slot 203.

[0046] In some embodiments, the base 202 and the cover 201 are respectively provided with a limiting groove 204 and a snap fastener 205. The base 202 and the cover 201 are detachably connected via the limiting groove 204 and the snap fastener 205. See also Figure 3 As shown, the base 202 has several limiting grooves 204, and the cover 201 has a snap fastener 205. The snap fastener 205 can be inserted into the corresponding limiting groove 204 under external force to achieve fixation and engagement. (See also...) Figure 1 As shown, several limiting grooves 204 and buckles 205 are respectively provided on both sides of the base 202 along its length.

[0047] In some embodiments, a recognition area is provided on the housing, within which a one-dimensional barcode and / or a two-dimensional barcode is provided for scanning and recognition by an external scanning device. The one-dimensional barcode and / or two-dimensional barcode is used for scanning and recording information by the scanning device. Preferably, the recognition area is located on the upper surface of the housing, for example, at one end of the upper surface of the housing.

[0048] In some of these embodiments, see Figure 1 As shown, at least one clearance groove 206 for positioning the housing is also provided on the outer wall of the housing. In use, the clearance groove 206 can cooperate with an external positioning post to fix its position. For example, in use, the housing is placed on a workbench or testing platform, and the external positioning post is engaged within the clearance groove 206 to fix the position of the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10, preventing movement of the ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card 10 during sample addition and detection, thus improving detection stability.

[0049] In some embodiments, ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 is assembled with detection reagents to form a kit.

[0050] The ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 includes the following steps when used:

[0051] (1) Before testing, turn on the dry fluorescence immunoassay analyzer, scan the QR code on the reagent kit with a barcode scanner, and select the corresponding “sample type” on the dry fluorescence immunoassay analyzer.

[0052] (2) Turn on the constant temperature incubator, set the reaction temperature to 37℃ and the reaction time to 10min. It can be used when the temperature rises to 37℃.

[0053] (3) Bring the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 and the spare sample to room temperature.

[0054] (4) Take out ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 from the aluminum foil packaging bag and use it within 30 minutes.

[0055] (5) Place the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 flat on the table, use a pipette to add 90 μL of the diluted sample to the sample well 2012 of the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10, and then insert the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 into the constant temperature incubator and incubate at 37°C for 10 min.

[0056] (6) After the reaction is complete, insert the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 into the detection card slot 203 of the dry fluorescence immunoassay analyzer as required, and click "Test" on the interface of the dry fluorescence immunoassay analyzer. The dry fluorescence immunoassay analyzer will automatically provide the quantitative test results. During the test, the QR code on the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 is detected by the dry fluorescence immunoassay analyzer, which can automatically call up the QR code information on the reagent kit.

[0057] This invention employs quantum dot microsphere fluorescence quantitative immunochromatography to detect ofloxacin residues in food. It leverages the high sensitivity, stability, high recovery rate, and convenience of quantum dot fluorescent microspheres. Furthermore, the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 features a unique one-dimensional or two-dimensional code, automatically identifying product information, retrieving the corresponding standard curve, and automatically providing quantitative test results, thus achieving rapid quantitative detection. Compared to quinolone detection cards and ratiometric fluorescent probe technology, this method is simpler and faster to operate, less affected by ambient temperature and solvent reagents, and provides accurate results, making it suitable for rapid food safety testing.

[0058] Compared with traditional technologies, the ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card 10 of this application has the following advantages:

[0059] (1) This application is easy to operate and can complete the experimental test and result interpretation well without good training and high skills.

[0060] (2) This application can perform rapid detection and quantification, and has a simple structure.

[0061] (3) The core of this application is to utilize quantum dot microsphere fluorescence immunochromatography technology and introduce it into a fluorescence quantitative rapid detection card to improve detection accuracy.

[0062] (4) The core of this application is to utilize quantum dot microsphere fluorescence immunochromatography technology and introduce it into a fluorescence quantitative rapid detection card to improve detection sensitivity.

[0063] (5) This application provides a quantum dot microsphere fluorescence immunochromatography technique, which has a simple sample pretreatment method, uses less reagents, causes less pollution, is inexpensive and does not use chloride-containing solvents.

[0064] (6) This application can analyze a wide range of drugs, including food additives, pesticides, veterinary drugs and other drugs.

[0065] (7) This application has the advantages of a wide quantitative detection range, a wide range of detection data, good repeatability, and good specificity.

[0066] (8) This application is less affected by the sample matrix and has a high recovery rate.

[0067] (9) The test card of this application has a simple structure, the standard curve can be imported through a one-dimensional code or a two-dimensional code, no on-site calibration is required, the sample can be tested as soon as it arrives, and it is convenient to use and fast to test.

[0068] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0069] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0070] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A rapid quantitative detection card for ofloxacin using quantum dot microspheres, characterized in that, The test strip includes a base plate and a sample pad, a fluorescent microsphere conjugate pad, a nitrocellulose membrane, and an absorbent pad, which are disposed on the base plate and connected in sequence. The nitrocellulose membrane has a detection line and a control line distributed at intervals. The detection line is closer to the fluorescent microsphere conjugate pad, and the control line is closer to the absorbent pad. The detection line is coated with a small molecule ofloxacin antigen, and the control line is coated with goat anti-mouse IgG antibody. The microsphere-labeled antibody conjugate in the fluorescent microsphere conjugate pad is a fluorescent microsphere-labeled ofloxacin antibody.

2. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to claim 1, characterized in that, The test strip has a long strip structure, and the sample pad, the fluorescent microsphere conjugate pad, the nitrocellulose membrane and the absorbent pad all extend along the length of the test strip.

3. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to claim 1, characterized in that, The length of the test strip is 6cm to 8cm; And / or, the width of the test strip is 2.5mm to 4mm.

4. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to any one of claims 1 to 3, characterized in that, The fluoxetine quantum dot microsphere fluorescence quantitative rapid detection card also includes a housing, on which a detection window and a sample application hole are provided. The test strip is assembled inside the housing, and the detection line and the quality control line on the test strip are located at the detection window position, and the sample pad is located at the sample application hole position.

5. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to claim 4, characterized in that, The size of the sample application port gradually decreases from one side located on the outer surface of the housing to the side located inside the housing.

6. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to claim 4, characterized in that, The detection window is elongated, and the detection line and the quality control line are located in the middle of the detection window.

7. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to claim 4, characterized in that, The housing includes a cover and a base, the cover and the base are detachably connected, the test strip is assembled on the base, and the cover is provided with a detection window and a sample application hole.

8. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to claim 7, characterized in that, The base is provided with a slot, and the test strip is inserted into the slot.

9. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card according to claim 7, characterized in that, The base and the cover are respectively provided with a limiting groove and a buckle, and the base and the cover are detachably connected through the limiting groove and the buckle.

10. The ofloxacin quantum dot microsphere fluorescence quantitative rapid detection card according to claim 4, characterized in that, The identification area on the housing is provided with a one-dimensional barcode and / or a two-dimensional barcode that can be scanned and identified by an external scanning device. And / or, at least one clearance groove for positioning the housing is also provided on the outer wall of the housing.

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