Mouse anesthesia scald experiment device

Abstract

The utility model relates to the technical field of mouse scald experiments, and particularly discloses a mouse anesthesia scald experiment device which comprises a water bath kettle, a support frame, a strip-shaped mouse placing groove body, an anesthesia air cylinder, an air anesthesia machine and an air guide pipe, a water storage pot is arranged at the top of the water bath pot; the bottom end of the supporting frame abuts against the inner bottom wall of the water storage pot. The mouse placing groove body is fixed on the supporting frame, and an oval scalding opening is formed in the inner bottom wall of the mouse placing groove body; the anesthesia inflator is axially fixed on the inner bottom wall of the mouse placing groove body, the open end of the anesthesia inflator is arranged corresponding to one side of the scald opening, and the closed end is provided with a through hole; an anesthetic gas outlet of the gas anesthesia machine is connected and communicated with one end of a gas guide pipe, and the other end of the gas guide pipe penetrates through the supporting frame and the through hole to be communicated with an inner cavity of the anesthetic gas cylinder; according to the mouse anesthesia scald experiment device, an experimenter is prevented from grasping a mouse with hands to conduct a scald experiment, safety is high, the mouse is scalded through the oval scald opening, and the scald area of the back of the mouse is easy to calculate.

Description

Technical Field

[0001] This utility model relates to the field of experimental technology of mouse burns, and more specifically to an experimental device for mouse anesthesia burns. Background Technology

[0002] The mouse scalding experiment is a commonly used medical research experiment. It allows observation of the pathophysiological mechanisms of scalded mice and the differences in the efficacy of different treatment methods, providing strong scientific evidence for the treatment and rehabilitation of burn patients. This experiment has broad application prospects in burn medicine, wound repair, and other fields. There are various scalding methods for mouse scalding experiments, including the hot water method, the steam method, and the copper rod method. Among these, the steam method and the hot water method are more commonly used. The steam method involves scalding the mouse's back with steam generated by heating, while the hot water method involves immersing the mouse's back in hot water.

[0003] In the experiment of scalding mice with hot water, healthy adult mice were first selected as experimental subjects. The mice were then anesthetized and the fur on their backs was removed. The experimenters then held the mice and placed them back down in hot water to scald them. However, this method has the following drawbacks: First, the experimenters are prone to burning their hands during the operation, which is not safe. Second, the outline of the scalded area on the mouse's back is irregular, making it difficult to calculate the scalded area on the mouse's back.

[0004] Therefore, in medical experiments involving scalding mice with hot water, how to provide a safe and easily controllable anesthetic scalding experimental device that can calculate the scalding area of ​​mice is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0005] In view of this, in order to solve the technical problems of lack of safety when experimental personnel handle mice to perform scalding, the irregular shape of the outline of the scald on the back of the mouse, and the difficulty in calculating the scald area, this utility model provides a mouse anesthesia scald experimental device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An experimental apparatus for anesthetic burns in mice includes a water bath, a support frame, a strip-shaped mouse placement tank, an anesthetic gas cylinder, an anesthesia machine, and a gas delivery tube. The water bath has a water reservoir at its top for storing hot water. The bottom end of the support frame abuts against the inner bottom wall of the water reservoir. The mouse placement tank is fixed to the support frame, and its radial cross-section is arc-shaped with the arc-shaped opening facing upwards. An elliptical burn wound is formed on the inner bottom wall of the mouse placement tank. The water level in the water reservoir is higher than the burn wound but lower than the opening of the mouse placement tank. The anesthetic gas cylinder is axially fixed to the inner bottom wall of the mouse placement tank, with its open end corresponding to the burn wound and a perforation at its closed end. The anesthetic gas outlet of the anesthesia machine is connected to one end of the gas delivery tube, and the other end of the gas delivery tube passes through the support frame and the perforation, communicating with the inner cavity of the anesthetic gas cylinder.

[0008] Based on the above technical solution, this utility model provides a mouse anesthesia scald experimental device. When scalding a mouse, the anesthetized mouse is first placed on a mouse placement tank, with its back covered and the scald wound exposed, and its head inserted into the anesthesia gas cylinder. Then, the gas anesthesia machine is activated, outputting anesthetic gas to the anesthesia gas cylinder to maintain the mouse in a continuous anesthetized state. Next, a support frame is placed in the water tank of a water bath, and the water level in the water tank is controlled to be above the scald wound but below the opening of the mouse placement tank. Finally, after standing for a period of time, the support frame is removed to complete the experiment. This utility model's mouse anesthesia scald experimental device uses a support frame instead of the experimenter's hands to hold the mouse during the scald experiment, avoiding burns to the experimenter's hands and improving safety. Furthermore, the elliptical scald wound makes the outline of the scald on the mouse's back more regular, making it easier to calculate the area of ​​the scald on the mouse's back. This device has a simple structure, reasonable design, and is easy to control and operate.

[0009] Preferably, the support frame includes two support plates, the bottom ends of which abut against the bottom wall of the water storage pot and are arranged opposite to each other, and the two ends of the mouse placement tank are respectively fixed to the lower part of the two support plates.

[0010] Preferably, a mouse anesthesia burn experimental device further includes a handle rod, which is arranged between the two support plates and its two ends are respectively fixed to the top of the two support plates.

[0011] The beneficial effect of adopting the above technical solution is that the support frame is more convenient to move and carry through the handle rod.

[0012] Preferably, the distance from the bottom of the handle to the lowest point of the inner wall of the mouse placement slot is 10cm.

[0013] Preferably, a mouse anesthesia burn experimental device further includes a mouse pressing component, which includes a connecting strap and a pressing block; the two ends of the connecting strap are respectively fixed to the upper parts of the two support plates; the pressing block is arranged vertically corresponding to the burn wound, with its top fixed to the connecting strap and its bottom able to apply pressure to the mouse to keep the mouse's body in an extended state.

[0014] The beneficial effect of using the above technical solution is that mice that have been anesthetized and are in a coma may slightly retract their limbs through physiological reactions. When the experimenter gently presses the pressure block with their fingers, the pressure block applies slight pressure to the mouse, keeping the mouse's body in an extended state.

[0015] Preferably, the connecting strip is made of silicone material.

[0016] Preferably, a mouse anesthesia burn experimental device further includes a limiting wire mesh, which is fixed to the bottom of the mouse placement tank and seals the burn wound to prevent the mouse from sliding out of the burn wound.

[0017] The beneficial effect of adopting the above technical solution is that the limiting wire mesh can not only prevent mice from slipping out of the scalded wound, but also prevent the surface of the mouse's back from being excessively immersed in hot water.

[0018] Preferably, the mouse placement tank and the two support plates are both acrylic plates.

[0019] Preferably, a mouse anesthesia burn experimental device further includes a positioning frame, which includes a U-shaped bracket and an arc-shaped connecting rod. There are two U-shaped brackets that are parallel to each other, and there are two arc-shaped connecting rods. The two ends of the two arc-shaped connecting rods are respectively fixed to the corresponding ends of the two U-shaped brackets. Each arc-shaped connecting rod is bent downward and its arc-shaped concave surface supports the mouse placement tank.

[0020] The advantage of adopting the above technical solution is that when the support frame is not in use, it can be stored on the positioning frame.

[0021] Preferably, the length of the mouse placement tank is 25cm-30cm, and the radius of the arc is 2cm-2.5cm. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0023] Figure 1The attached diagram is a schematic diagram of the present invention in operation;

[0024] Figure 2 The attached figure is a schematic diagram showing the connection between the support frame, the mouse placement tank, the handle rod, and the mouse clamping component in this utility model;

[0025] Figure 3 The attached figure is a top view of the mouse placement tank in this utility model;

[0026] Figure 4 The attached figure is a structural schematic diagram of the positioning frame in this utility model;

[0027] Figure 5 This is a schematic diagram showing the support frame, mouse placement tank, handle rod, and mouse clamping device stored on the positioning frame in this utility model.

[0028] In the diagram: 1-Water bath, 2-Support frame, 3-Mouse placement tank, 4-Anesthetic cylinder, 5-Anesthetic machine, 6-Handle rod, 7-Mouse clamp, 8-Limiting wire mesh, 9-Positioning frame, 21-Support plate, 31-Burn wound, 71-Connecting strap, 72-Pressure block, 91-U-shaped bracket, 92-Arc-shaped connecting rod. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0031] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0032] See appendix Figures 1 to 5 This invention relates to a mouse anesthesia and scalding experimental apparatus, comprising a water bath 1, a support frame 2, a strip-shaped mouse placement tank 3, an anesthesia gas cylinder 4, an anesthesia machine 5, and a gas delivery tube. The top of the water bath 1 is equipped with a water reservoir for storing hot water. The bottom end of the support frame 2 abuts against the inner bottom wall of the water reservoir. The mouse placement tank 3 is fixed on the support frame 2, and the radial cross-section of the mouse placement tank 3 is arc-shaped with its arc-shaped opening facing upwards. An elliptical scalding wound 31 is formed on the inner bottom wall of the mouse placement tank 3. The water level in the water reservoir is higher than the scalding wound 31 and lower than the opening end of the mouse placement tank 3. The anesthesia gas cylinder 4 is axially fixed on the inner bottom wall of the mouse placement tank 3, with its open end corresponding to one side of the scalding wound 31 and a perforation on its closed end. The anesthesia gas outlet of the anesthesia machine 5 is connected to one end of the gas delivery tube, and the other end of the gas delivery tube passes through the support frame 2 and the perforation to communicate with the inner cavity of the anesthesia gas cylinder 4. The temperature of the hot water in the water bath needs to be controlled between 80℃ and 90℃.

[0033] The water bath is model DK-S22; the air anesthesia machine is model MSS-3.

[0034] In some specific examples, the support frame 2 includes two support plates 21, the bottom ends of which abut against the bottom wall of the water storage pot and are arranged opposite to each other, and the two ends of the mouse placement tank 3 are respectively fixed to the lower part of the two support plates 21.

[0035] In some embodiments, a mouse anesthesia burn experimental device further includes a handle 6, which is arranged between two support plates 21 and its two ends are respectively fixed to the top of the two support plates 21.

[0036] Specifically, the distance from the bottom of the handle 6 to the lowest point of the inner wall of the mouse placement tank 3 is 10cm.

[0037] In some embodiments, a mouse anesthesia burn experimental device further includes a mouse pressing component 7, which includes a connecting strap 71 and a pressing block 72. The two ends of the connecting strap 71 are respectively fixed to the upper parts of two support plates 21. The pressing block 72 is arranged vertically and vertically corresponding to the burn wound 31, with its top fixed to the connecting strap 71 and its bottom able to apply pressure to the mouse to keep the mouse's body in an extended state.

[0038] Specifically, the connecting strip 71 is made of silicone material.

[0039] In some embodiments, a mouse anesthesia burn experimental device further includes a limiting wire mesh 8, which is fixed to the bottom of the mouse placement tank 3 and seals the burn wound 31 to prevent the mouse from sliding out of the burn wound 31.

[0040] Specifically, the mouse placement tank 3 and the two support plates 21 are both acrylic plates.

[0041] In some embodiments, a mouse anesthesia burn experimental device further includes a positioning frame 9, which includes a U-shaped bracket 91 and an arc-shaped connecting rod 92; there are two U-shaped brackets 91 that are parallel to each other; there are two arc-shaped connecting rods 92, and the two ends of the two arc-shaped connecting rods 92 are respectively fixed to the corresponding ends of the two U-shaped brackets 91, and each arc-shaped connecting rod 92 is bent downward and its arc-shaped concave surface supports the mouse placement tank 3.

[0042] Specifically, the length of the mouse placement tank 3 is 25cm-30cm, and the radius of the arc is 2cm-2.5cm.

[0043] When using this device, firstly, select a healthy adult mouse, anesthetize it, and remove the fur from its back. Cover the burn wound in the mouse's tank with its back, leaving the burn wound exposed. Place the mouse's head into the anesthesia cylinder. Then, start the gas anesthesia machine, which delivers anesthetic gas to the cylinder to keep the mouse in a comatose state. Next, place the support frame in the water tank of the water bath, ensuring the water level is above the burn wound but below the rim of the mouse's tank. Finally, let it stand for 5 to 10 seconds, then remove the support frame to complete the burn experiment. During the experiment, the experimenter can gently press the top of the pressure block, applying slight pressure to the mouse from top to bottom to prevent it from curling up its limbs due to physiological reactions.

[0044] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A mouse anesthesia burn experimental apparatus, characterized in that, include: Water bath (1), the top of which is provided with a water storage tank for storing hot water; Support frame (2), the bottom end of which abuts against the inner bottom wall of the water storage pot; A strip-shaped mouse placement tank (3) is fixed on the support frame (2). The radial cross-section of the mouse placement tank (3) is arc-shaped with its arc-shaped groove facing upward. An elliptical scald wound (31) is provided on the inner bottom wall of the mouse placement tank (3). The water level in the water storage pot is higher than the scald wound (31) and lower than the groove end of the mouse placement tank (3). Anesthesia cylinder (4), the anesthesia cylinder (4) is axially fixed on the inner bottom wall of the mouse placement tank (3), its open end is arranged on the side of the burn wound (31), and a perforation is provided on the closed end; The gas anesthesia machine (5) and the gas delivery tube are provided. The anesthetic gas outlet of the gas anesthesia machine (5) is connected to one end of the gas delivery tube and communicates with it. The other end of the gas delivery tube passes through the support frame (2) and the perforation and communicates with the inner cavity of the anesthetic gas cylinder (4).

2. The experimental apparatus for anesthetic burns in mice according to claim 1, characterized in that, The support frame (2) includes two support plates (21), the bottom ends of the two support plates (21) abut against the bottom wall of the water storage pot and are arranged opposite to each other, and the two ends of the mouse placement tank (3) are respectively fixed to the lower part of the two support plates (21).

3. The mouse anesthesia burn experimental apparatus according to claim 2, characterized in that, It also includes a handle (6), which is arranged between the two support plates (21) and its two ends are respectively fixed to the top of the two support plates (21).

4. The mouse anesthesia burn experimental apparatus according to claim 3, characterized in that, The distance from the bottom of the handle (6) to the lowest point of the inner wall of the mouse placement tank (3) is 10cm.

5. The mouse anesthesia burn experimental apparatus according to claim 2, characterized in that, It also includes a mouse clamp (7), which comprises: A connecting strap (71) is provided, with both ends of the connecting strap (71) fixed to the upper parts of the two support plates (21); The pressure block (72) is arranged vertically to the burn wound (31), with its top fixed to the connecting strap (71) and its bottom able to apply pressure to the mouse to keep the mouse's body in an extended state.

6. The mouse anesthesia burn experimental apparatus according to claim 5, characterized in that, The connecting strip (71) is made of silicone material.

7. The experimental apparatus for scalding mice under anesthesia according to claim 1, characterized in that, It also includes a limiting wire mesh (8), which is fixed to the bottom of the mouse placement tank (3) and seals the burn wound (31) to prevent the mouse from sliding out of the burn wound (31).

8. The mouse anesthesia burn experimental apparatus according to claim 2, characterized in that, The mouse placement tank (3) and the two support plates (21) are both acrylic plates.

9. A mouse anesthesia burn experimental apparatus according to any one of claims 1 to 8, characterized in that, It also includes a positioning frame (9), which comprises: U-shaped brackets (91), wherein there are two U-shaped brackets (91) and they are parallel to each other; Two arc-shaped connecting rods (92) are provided. The two ends of the two arc-shaped connecting rods (92) are respectively fixed to the corresponding ends of the two U-shaped brackets (91). Each arc-shaped connecting rod (92) is bent downward and its arc-shaped concave surface supports the mouse placement groove (3).

10. A mouse anesthesia burn experimental apparatus according to any one of claims 1 to 8, characterized in that, The length of the mouse placement tank (3) is 25cm-30cm, and the radius of the arc is 2cm-2.5cm.

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