Agarose gel plate shaping tool

By using a vibrating motor and a suspension support in the shaping tool, the problem of air bubbles in the preparation of agarose gel plates was solved, achieving the preparation of air bubble-free, high-purity gel plates, and enabling precise control of gel plate size.

CN223545585UActive Publication Date: 2025-11-14WUHAN GEOCHROM BIOTECH CO LTD
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Patent Information

Application Number
CN202423202712.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-12-25
Publication Date
2025-11-14
Estimated Expiration
2034-12-25

AI Technical Summary

Technical Problem

The existing agarose gel plate preparation process cannot remove air bubbles in time, resulting in low visibility and purity of the finished product.

Method used

A shaping tool consisting of a molding box, a vibration motor, and a suspension support is used. The vibration motor drives the molding box to vibrate to expel air bubbles, and the width and thickness of the gel plate are controlled by a partition plate and a grid plate.

Benefits of technology

This method produces bubble-free agarose gel plates, ensuring their visibility and purity, and allowing for precise control of the plate size.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses an agarose gel plate shaping tool. The agarose gel plate shaping tool comprises a forming box and a vibration motor, the forming box is of a cuboid box body structure with an upper opening, a vibration motor is fixed to the bottom of the forming box, and the whole forming box is supported by a suspension support in a floating mode. A plurality of groups of symmetrically distributed caulking grooves are formed in the long side surface of the forming box, and isolation plates or grid plates can be selectively inserted into the symmetrical caulking grooves; the structure of the grid plate is consistent with that of the isolation plate, the grid plate and the isolation plate have the same upper barrier strip, side rubber strip and barrier strip rubber strip structures, no embedded rubber strip is arranged, and the grid plate is provided with arrayed strip-shaped openings to form grid strip holes; the device has the beneficial effects that the width and the thickness of the agarose gel plate can be controlled; and the visibility and the purity of the agarose gel plate finished product are ensured.
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Description

Technical Field

[0001] This utility model belongs to the field of agarose gel plate manufacturing technology, and specifically relates to an agarose gel plate shaping tool. Background Technology

[0002] Agarose gel electrophoresis is a commonly used tool in biological experiments. Currently, laboratories prepare gel plates on demand, using plastic boxes as molds, pouring agarose gel liquid into them, and allowing it to solidify to form an agarose gel plate. There are also existing devices for forming agarose gel plates, such as the patent document with publication number CN2601403Y.

[0003] The aforementioned equipment cannot remove air bubbles from the agarose gel solution in a timely manner during the preparation of agarose gel plates. As a result, air bubbles remain inside the solidified agarose gel solution, leading to low visibility and purity of the finished agarose gel plate. Utility Model Content

[0004] The purpose of this invention is to provide an agarose gel plate shaping tool to solve the technical problems mentioned in the background section.

[0005] The above-mentioned technical objective of this utility model is achieved through the following technical solution:

[0006] A tool for shaping agarose gel plates includes: a molding box and a vibration motor; the molding box is a rectangular box structure with an open top, and a vibration motor is fixed at the bottom of the molding box. The molding box is supported by a floating bracket; several sets of symmetrically distributed slots are provided on the long side of the molding box, and a partition plate or a grid plate can be inserted into the symmetrical slots; the partition plate and the grid plate are parallel to the short side of the molding box; the main body of the partition plate is a solid plate with a plate-like structure, and an upper baffle strip with a width greater than the solid plate is provided on the upper part of the solid plate. Side adhesive strips are attached and fixed on both sides of the solid plate, and a baffle strip is attached and fixed below the upper baffle strip. An embedded adhesive strip is embedded in the bottom of the side adhesive strip and the solid plate. The lower part of the embedded adhesive strip protrudes to the bottom of the side adhesive strip and the solid plate and remains parallel to the lower surface of the solid plate. The structure of the grid plate is the same as that of the partition plate, and both have the same upper baffle strip, side adhesive strip, and baffle strip structure. The grid plate does not have an embedded adhesive strip. An array of strip openings is provided on the grid plate to form grid holes.

[0007] Furthermore, to facilitate the vibration and floating of the molding box, the suspension support includes: a base frame, a lower support, an upper support, spring pieces, spring supports, and springs; wherein the base frame is a horizontal support structure, and several inclined spring pieces are fixed on the base frame by the lower support. The top of the spring pieces is fixed to the bottom of the molding box by the upper support. A spring support is fixed to the side of each set of upper supports. The spring support and the bottom of the spring piece are connected by a spring. Under no external force, the spring is normally not compressed or stretched.

[0008] Furthermore, to facilitate the insertion of the isolation plate and the grid plate, the main body of the groove is a side groove that is embedded in the side of the isolation plate and the grid plate; the top of the side groove is recessed downward to form a top groove, and the upper stop strip of the isolation plate and the grid plate is embedded in the top groove.

[0009] Furthermore, to facilitate the determination of the thickness of the agarose gel plate, a thickness scale line for indicating the thickness of the agarose gel plate is provided on the side of the side groove.

[0010] Furthermore, to facilitate the cleaning of gel fragments, rough edges, and defective products in the molding box after the agarose gel plate has been used up, an opening is provided at the bottom of one end of the molding box, and a bucket-shaped recycling hopper is connected and fixed below the opening. A receiving device (such as a receiving bag or container) is provided below the recycling hopper.

[0011] Furthermore, to facilitate the determination of the thickness of the agarose gel plate, the top surface of the long side of the molding box is provided with linearly distributed fixed-width scale lines.

[0012] Furthermore, in order to facilitate the cooling of the agarose gel liquid in the molding box and accelerate its solidification, a fan bracket is provided on the side of the molding box away from the recycling hopper, and several fans are fixed on the fan bracket, with the air outlet of the fans facing the side towards the recycling hopper.

[0013] Furthermore, to facilitate the hanging of the receiving bag below the recycling hopper, several hooks for hanging the receiving bag are provided at the lower opening of the recycling hopper.

[0014] Furthermore, in order to prevent gel fragments, burrs, and defective products from falling from the lower opening of the recycling hopper to the suspension support and making them inconvenient to clean when a receiving box is set below the recycling hopper, an inclined blocking plate is provided on the side of the lower opening of the recycling hopper near the suspension support, and the blocking plate is inclined towards the center of the recycling hopper.

[0015] In summary, this utility model has the following beneficial effects:

[0016] ① The width and thickness of the agarose gel plate can be controlled: By setting up the separator plate, grid plate, and thickness scale line, the separator plate is inserted into the corresponding groove according to the required width of the agarose gel plate. The solid side of the separator plate is embedded in the side groove. At this time, the side rubber strips on both sides of the solid plate are elastically compressed. The side of the solid plate and the side groove are sealed by interference fit. Continue to press down the separator plate until the upper stop strip on the separator plate is embedded in the top groove. At this time, the bottom inner rubber strip is squeezed and compressed to seal the bottom of the separator plate and the bottom of the molding box. The distance between adjacent separator plates can be determined according to the scale reading of the width scale line at the position of the separator plate, thereby determining whether the required width of the agarose gel plate has reached the predetermined width. Then, the prepared melted agarose gel liquid is poured into the cavity formed by the separator plate, grid plate, and side wall of the molding box. The liquid level of the poured agarose gel liquid is determined by the thickness scale line at the groove, thereby determining the thickness of the solidified agarose gel plate.

[0017] ② The formed agarose gel plate is free of air bubbles, ensuring the visibility and purity of the finished agarose gel plate: By setting up a vibration motor and a suspension support, after the agarose gel liquid is poured out, the vibration motor is powered on and put into operation. The vibration motor drives the forming box and the agarose gel liquid inside to vibrate. According to the principle of tamping, the air bubbles in the agarose gel liquid are shaken out, ensuring that there are no air bubbles inside the formed agarose gel plate, thereby ensuring the visibility and purity of the finished agarose gel plate. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the structure of the suspension support in this utility model;

[0020] Figure 3 This is a schematic diagram of the structure of the recycling hopper in this utility model;

[0021] Figure 4 This is a schematic diagram of the insertion principle of the isolation plate and the grid plate in this utility model;

[0022] Figure 5 yes Figure 4 Enlarged view of the structure at point A in the middle;

[0023] Figure 6 This is a schematic diagram of the structure of the isolation plate in this utility model;

[0024] Figure 7 yes Figure 6 Enlarged view of the structure at point B in the middle;

[0025] Figure 8 This is a schematic diagram of the structure of the grid plate in this utility model;

[0026] Figure 9 This is a schematic diagram of the structure of the grid plate in this utility model, which simultaneously fabricates multiple agarose gel plates;

[0027] Figure 10 This is a schematic diagram of the installation structure of the cooling fan in this utility model.

[0028] In the diagram, 1. Molding box; 2. Vibration motor; 3. Suspension bracket; 4. Isolation plate; 5. Grid plate; 6. Recycling hopper; 7. Fan bracket; 8. Fan; 9. Insert groove; 10. Agarose gel plate; 11. Width scale line; 31. Base frame; 32. Lower support; 33. Upper support; 34. Spring piece; 35. Spring support; 36. Spring; 41. Solid plate; 42. Upper baffle; 43. Side rubber strip; 44. Baffle rubber strip; 45. Embedded rubber strip; 51. Grid hole; 61. Hook; 62. Barrier inclined plate; 91. Side insert groove; 92. Top insert groove; 93. Thickness scale line; 101. Break line. Detailed Implementation

[0029] The present invention will be further described in detail below with reference to the accompanying drawings.

[0030] Example:

[0031] Please see Figures 1-10 The present invention provides the following technical solution:

[0032] A tool for shaping agarose gel plates includes: a molding box 1 and a vibration motor 2; the molding box 1 is a rectangular box structure with an open top, and a vibration motor 2 is fixed at the bottom of the molding box 1; the molding box 1 is floated and supported by a suspension bracket 3; several sets of symmetrically distributed slots 9 are provided on the long side of the molding box 1, and a partition plate 4 or a grid plate 5 can be inserted into the symmetrical slots 9; the partition plate 4 and the grid plate 5 are parallel to the short side of the molding box 1; the main body of the partition plate 4 is a solid plate 41 with a plate-like structure, and the upper part of the solid plate 41 is provided with a width greater than the solid plate. The upper baffle 42 of 41 has side adhesive strips 43 attached and fixed on both sides of the solid plate 41, and a baffle strip 44 attached and fixed below the upper baffle 42. The side adhesive strips 43 and the bottom of the solid plate 41 are together embedded with an embedded adhesive strip 45. The lower part of the embedded adhesive strip 45 protrudes to the bottom of the side adhesive strips 43 and the solid plate 41 and remains parallel to the lower surface of the solid plate 41. The structure of the grid plate 5 is the same as that of the partition plate 4, both having the same upper baffle, side adhesive strip, and baffle strip structure. The embedded adhesive strip 4 is not provided. The grid plate 5 is provided with an array of strip openings to form grid holes 51.

[0033] The suspended support 3 includes: a base frame 31, a lower support 32, an upper support 33, a spring piece 34, a spring support 35, and a spring 36; wherein the base frame 31 is a horizontal support structure, and several inclined spring pieces 34 are fixed on the base frame 31 by the lower support 32. The top of the spring piece 34 is fixed to the bottom of the molding box 1 by the upper support 33. A spring support 35 is fixed to the side of each set of upper supports 33. A spring support 36 is connected to the lower part of the spring support 35 and the spring piece 34. Under normal conditions, the spring 36 is not compressed or stretched when no external force is applied.

[0034] The main body of the groove 9 is a side groove 91 that is embedded in the side of the isolation plate 4 and the grid plate 5; the top of the side groove 91 is recessed downward to form a top groove 92, and the upper baffle 42 of the isolation plate 4 and the grid plate 5 is embedded in the top groove 92.

[0035] The side groove 91 is provided with a thickness scale line 93 for indicating the thickness of the agarose gel plate.

[0036] The molding box 1 has an opening at one end of the bottom, and a bucket-shaped recycling hopper 6 is fixedly connected below the opening. A receiving device (such as a receiving bag or a container) is provided below the recycling hopper 6.

[0037] The molding box 1 has linearly distributed fixed-width scale lines 11 on the top surface of its long side.

[0038] The molding box 1 is provided with a fan bracket 7 on the side away from the recycling hopper 6. Several fans 8 are fixed on the fan bracket 7, and the air outlet of the fans 8 faces the side facing the recycling hopper 6.

[0039] The lower opening of the recycling hopper 6 is equipped with several hooks 61 for hanging the receiving bags.

[0040] The lower opening of the recovery hopper 6 is provided with an inclined baffle plate 62 on the side near the suspension support 3, and the baffle plate 62 is inclined towards the center of the recovery hopper 6.

[0041] Brief description of usage:

[0042] In use, the separator plate 4 is inserted into the corresponding groove 9 according to the required width of the agarose gel plate. The side of the solid plate 41 of the separator plate 4 is embedded in the side groove 91. At this time, the side adhesive strips 43 on both sides of the solid plate 41 are elastically compressed, and the sides of the solid plate 41 and the side groove 91 are sealed by an interference fit. The separator plate 4 is pressed down until the upper stop strip 42 on the separator plate 4 is embedded in the top groove 92. At this time, the bottom inner adhesive strip 45 is squeezed and compressed to seal the bottom of the separator plate 4 and the bottom of the molding box 1. The spacing between adjacent separator plates 4 can be determined according to the scale reading of the fixed width scale line 11 at the location of the separator plate 4, thereby determining whether the required width of the agarose gel plate has reached the predetermined width.

[0043] If multiple agarose gel plates need to be formed simultaneously, the separator plate 4 between two or more agarose gel plates can be replaced with a grid plate 5. The installation method of the grid plate 5 is the same as that of the separator plate 4, so it will not be described again.

[0044] Then, the fully melted agarose gel solution is poured into the cavity formed by the isolation plate 4, the grid plate 5, and the side wall of the molding box 1. The liquid level of the poured agarose gel solution is determined by the thickness scale line 93 at the groove 9, thereby determining the thickness of the solidified agarose gel plate 10.

[0045] After the agarose gel solution is poured out, the vibration motor 2 is powered on and put into operation. The vibration motor 2 drives the molding box 1 and the agarose gel solution inside to vibrate. According to the principle of tamping, the air bubbles in the agarose gel solution are shaken out, ensuring that there are no air bubbles inside the molded agarose gel plate 10, thereby ensuring the visibility and purity of the finished agarose gel plate 10.

[0046] When the vibration motor 2 drives the molding box 1 to vibrate, the molding box 1 drives the top of the spring 34 to swing through the upper support 33. The spring 36 is used to constrain the vibration amplitude of the molding box 1 and uses its elasticity to reset the molding box 1 after each vibration. After the bubbles in the agarose gel solution are released, the vibration motor 2 is turned off, the molding box 1 stops vibrating and resets.

[0047] At this point, you can wait for the agarose gel to cool and solidify naturally. If you need to shorten the cooling and solidification time of the agarose gel, you can power on the fan 8 and make it work. The fan 8 will blow away the heat near the agarose gel to accelerate its cooling. After the agarose gel has cooled and solidified, an agarose gel plate 10 is formed.

[0048] At this point, the agarose gel plate 10 can be used. If a grid plate 5 is provided, such as... Figure 9 As shown, the grid plate 5 is pulled out from its groove 9. Due to the conduction principle of the grid bar hole 51, the thickness of the agarose gel plates 10 on both sides of the grid plate 5 is the same. After the grid plate 5 is pulled out, a dashed fracture line 101 will be formed on the agarose gel plate 10. When not in use, multiple agarose gel plates 10 are connected together by the fracture line 101. When one or more agarose gel plates 10 need to be used, the fracture line 101 is broken by hand, and the agarose gel plates 10 of the same thickness are separated.

[0049] After the above operations are completed, remove all the isolation plates 4 and grid plates 5, and use a brush or cloth to sweep the gel fragments, burrs, defective products, etc. into the recycling hopper 6 and discharge them from the lower opening of the recycling hopper 6. The receiving mechanism below the recycling hopper 6 (a receiving bag is suspended on the hook or a receiving box is set below the recycling hopper 6) will receive them.

[0050] This specific embodiment is merely an explanation of the present utility model and is not intended to limit the present utility model. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but as long as they are within the scope of the claims of the present utility model, they are protected by patent law.

Claims

1. An agarose gel plate setting tool, comprising: A molding box (1) and a vibration motor (2); characterized in that: the molding box (1) is a rectangular box structure with an open top, a vibration motor (2) is fixed at the bottom of the molding box (1), and the molding box (1) is supported by a floating bracket (3); the long side of the molding box (1) is provided with several sets of symmetrically distributed slots (9), and a partition plate (4) or a grid plate (5) can be inserted into the symmetrical slots (9); the partition plate (4) and the grid plate (5) are parallel to the short side of the molding box (1); the main body of the partition plate (4) is a solid plate (41) with a plate-like structure, and the upper part of the solid plate (41) is provided with an upper part with a width greater than that of the solid plate (41). The side strips (43) are attached and fixed on both sides of the solid plate (41), and the bottom of the upper strip (42) is attached and fixed with a strip (44). The bottom of the side strip (43) and the solid plate (41) are together embedded with an embedded strip (45). The lower part of the embedded strip (45) extends to the bottom of the side strip (43) and the solid plate (41) and remains parallel to the lower surface of the solid plate (41). The structure of the grid plate (5) is the same as that of the partition plate (4), both having the same upper strip, side strip, and strip structure. The embedded strip (45) is not provided. The grid plate (5) is provided with an array of strip openings to form grid holes (51).

2. The agarose gel plate shaping tool according to claim 1, characterized in that: The suspended support (3) includes: a base frame (31), a lower support (32), an upper support (33), a spring piece (34), a spring support (35), and a spring (36); wherein the base frame (31) is a horizontal support structure, and a number of inclined spring pieces (34) are fixed on the base frame (31) by the lower support (32). The top of the spring piece (34) is fixed to the bottom of the molding box (1) by the upper support (33). A spring support (35) is fixed on the side of each set of upper supports (33). The spring support (35) and the spring piece (34) are connected by a spring (36) below. Under no external force, the spring (36) is normally not compressed or stretched.

3. The agarose gel plate shaping tool according to claim 1, characterized in that: The main body of the groove (9) is a side groove (91) embedded in the side of the isolation plate (4) and the grid plate (5); the top of the side groove (91) is recessed downward to form a top groove (92), and the upper baffle (42) of the isolation plate (4) and the grid plate (5) is embedded in the top groove (92).

4. The agarose gel plate shaping tool according to claim 3, characterized in that: The side groove (91) is provided with a thickness scale line (93) for indicating the thickness of the agarose gel plate.

5. The agarose gel plate shaping tool according to claim 1, characterized in that: The molding box (1) has an opening at one end of its bottom, and a bucket-shaped recycling bucket (6) is fixedly connected below the opening. A receiving device is provided below the recycling bucket (6).

6. The agarose gel plate shaping tool according to claim 1, characterized in that: The molding box (1) has linearly distributed fixed-width scale lines (11) on the top surface of its long side.

7. The agarose gel plate shaping tool according to claim 5, characterized in that: The molding box (1) is provided with a fan bracket (7) on the side away from the recycling hopper (6). Several fans (8) are fixed on the fan bracket (7), and the air outlet of the fans (8) faces the side facing the recycling hopper (6).

8. The agarose gel plate shaping tool according to claim 5, characterized in that: The lower opening of the recycling hopper (6) is provided with several hooks (61) for hanging the receiving bags.

9. The agarose gel plate shaping tool according to claim 5, characterized in that: The lower opening of the recovery hopper (6) is provided with an inclined baffle plate (62) on the side near the suspension support (3), and the baffle plate (62) is inclined toward the center of the recovery hopper (6).

Citation Information

Patent Citations

  • Agarose gel plate forming appts.

    CN2601403Y