Protein purification and impurity washing device

Abstract

The utility model provides a protein purification and impurity washing device which comprises a base, a rotating plate is rotatably arranged at the top of one end of the base in a limiting mode, rotating cylinders are symmetrically and rotatably arranged on the two sides of the top of the rotating plate, and filter cylinders are installed in the rotating cylinders through positioning mechanisms. And a jacking mechanism is arranged below the filter cylinder and corresponds to the interior of the rotating cylinder. The upper cover can be subjected to ascending adjustment through a hydraulic rod and a mounting cover, so that the rotating cylinders are separated from the upper cover, the two rotating cylinders can be rotated and alternately adjusted through a rotating plate, and the filter cylinders can be ejected out of the rotating cylinders through jacking mechanisms such as a fixed frame, a movable cover and a spring, so that filtered impurities and the like can be conveniently cleaned; and meanwhile, centrifugal purification can be carried out on the other rotating cylinder, the protein purification efficiency can be improved, and the practicability is high.

Description

Technical Field

[0001] This utility model relates to the field of protein purification technology, specifically a protein purification and washing device. Background Technology

[0002] Protein purification is the process of separating a target protein from other proteins and non-protein substances by utilizing the similarities and differences between different proteins through a series of technical means.

[0003] Chinese utility model patent CN220132118U discloses a protein purification device, which includes a purification unit comprising a base, a mounting frame, a purification tank, a feed hopper, a control valve, a discharge pipe, a fixing rod, a fixing cylinder, a bearing, a damping telescopic rod, a support foot, a rubber pad, and a motor. The motor drives the purification tank to rotate within the bearing for centrifugation. The fixing rod and fixing cylinder limit the rotation of the purification tank, preventing it from shaking due to centrifugal force and thus ensuring that the purification process is not affected by shaking. However, this device is inconvenient for removing impurities after separation from the purification tank, which affects the protein purification efficiency and reduces its practicality.

[0004] Therefore, this utility model provides a protein purification and washing device. Utility Model Content

[0005] To address the shortcomings of existing technologies, the purpose of this invention is to provide a protein purification and impurity washing device to solve the problems mentioned in the background. This invention uses a rotating plate to adjust the rotation of the rotating cylinder, allowing the rotating cylinder to separate from the top cover, making it easy to remove the filter cartridge from the inside of the rotating cylinder. This facilitates the cleaning of filtered impurities, etc. It is simple to operate, can improve the protein purification efficiency, and is highly practical.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a protein purification and washing device, comprising a base, a rotating plate rotatably mounted at the top of one end of the base, rotating cylinders symmetrically mounted on both sides of the top of the rotating plate, a filter cylinder mounted inside the rotating cylinder via a positioning mechanism, a lifting mechanism positioned below the filter cylinder corresponding to the interior of the rotating cylinder, the lifting mechanism comprising a fixed frame and a movable cover, an L-shaped bracket fixedly mounted at the top of the other end of the base, and an upper cover movably mounted below the top of the L-shaped bracket via a hydraulic rod, the upper cover positioned above the rotating cylinder.

[0007] Furthermore, the fixed frame is fixedly installed at the bottom of the inner side of the rotating cylinder, the movable cover is movably installed on the outer side of the fixed frame, and a spring is fixedly installed between the inner top wall of the movable cover and the bottom wall of the rotating cylinder.

[0008] Furthermore, the filter cartridge is placed on top of the movable cover and in close contact with it, the spring is set inside the fixed frame, and a drain port is fixedly provided on one side of the movable cover corresponding to the inside of the rotating cylinder.

[0009] Furthermore, the positioning mechanism includes a fixing ring, which is fixedly disposed on the inner wall of the rotating cylinder, and the fixing ring has symmetrical insertion holes at both ends.

[0010] Furthermore, fixing blocks are symmetrically fixed on the outer walls of both sides of the top of the filter cartridge, and insertion rods are fixedly installed at the bottom of the fixing blocks, with the bottom end of the insertion rods inserted into the inside of the insertion hole.

[0011] Furthermore, the hydraulic rod is fixedly mounted on the top of the L-shaped bracket, and a mounting cover is fixedly mounted on the bottom end of the output rod of the hydraulic rod. The upper cover is rotatably limited and mounted at the bottom of the mounting cover.

[0012] Furthermore, limit rods are symmetrically fixed on both sides of the top of the mounting cover, and the top of the limit rods is slidably disposed inside the L-shaped bracket. A servo motor is fixedly installed at the center of the inner top wall of the mounting cover, and the top of the output shaft of the servo motor is fixedly connected to it.

[0013] Furthermore, a protrusion is fixedly provided at the bottom center of the top cover, and the protrusion is pressed and contacted with the top of the filter cartridge. A positioning rod is symmetrically fixedly provided at the bottom edge of the top cover corresponding to the outer side of the protrusion. The top of the rotating cylinder has a circular positioning hole, and the positioning rod is inserted and installed inside the positioning hole.

[0014] The beneficial effects of this utility model are as follows: This utility model provides a protein purification and impurity washing device. The upper cover can be raised and adjusted using a hydraulic rod and mounting cover, allowing the rotating cylinder to separate from the upper cover. A rotating plate allows for the rotation and alternating adjustment of two rotating cylinders. A lifting mechanism consisting of a fixed frame, movable cover, and springs can push the filter cartridge out from inside the rotating cylinder, facilitating the cleaning of filtered impurities. The device is simple to operate and can simultaneously perform centrifugal purification on the other rotating cylinder, improving protein purification efficiency and practicality. Furthermore, the positioning mechanism, including a fixed ring, insertion hole, insertion rod, and fixed ring, allows for the positioning and installation of the filter cartridge, as well as limiting its movement, thus improving the stability of the filter cartridge. Attached Figure Description

[0015] Figure 1 This is a structural diagram of a protein purification and washing device according to the present invention;

[0016] Figure 2 This is a front cross-sectional view of a protein purification and washing device according to the present invention;

[0017] Figure 3This is a cross-sectional view of the centrifuge mechanism of a protein purification and washing device according to this utility model;

[0018] Figure 4 This is a structural diagram of the upper cover of a protein purification and washing device according to the present invention;

[0019] In the diagram: 1. Base; 2. Rotating plate; 3. Rotating cylinder; 4. L-shaped bracket; 5. Top cover; 6. Mounting cover; 7. Hydraulic rod; 8. Limiting rod; 9. Filter cartridge; 10. Drain port; 11. Positioning hole; 12. Positioning rod; 13. Servo motor; 14. Fixed frame; 15. Movable cover; 16. Spring; 17. Fixing block; 18. Insert rod; 19. Fixing ring. Detailed Implementation

[0020] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0021] Please see Figures 1 to 4 This utility model provides a technical solution: a protein purification and impurity washing device, including a base 1. A rotating plate 2 is rotatably mounted on the top of one end of the base 1. Rotating cylinders 3 are symmetrically mounted on both sides of the top of the rotating plate 2. A filter cartridge 9 is installed inside the rotating cylinder 3 through a positioning mechanism. A lifting mechanism is provided below the filter cartridge 9 corresponding to the inside of the rotating cylinder 3. The lifting mechanism includes a fixed frame 14 and a movable cover 15. An L-shaped bracket 4 is fixedly mounted on the top of the other end of the base 1. A top cover 5 is movably mounted below the top of the L-shaped bracket 4 through a hydraulic rod 7. The top cover 5 is positioned above the rotating cylinder 3. The top cover 5 and the rotating cylinder 3 are designed separately. After the top cover 5 is separated from the rotating cylinder 3, it is convenient to remove the filter cartridge 9 from the inside of the rotating cylinder 3, thereby facilitating the cleaning of impurities without affecting the subsequent purification of proteins, thus improving the purification and impurity washing efficiency of proteins.

[0022] In this embodiment, the fixed frame 14 is fixedly installed at the bottom of the inner side of the rotating cylinder 3, and the movable cover 15 is movably installed on the outer side of the fixed frame 14. A spring 16 is fixedly installed between the inner top wall of the movable cover 15 and the bottom wall of the rotating cylinder 3. The filter cartridge 9 is placed on the top of the movable cover 15 and in close contact. The spring 16 is installed inside the fixed frame 14. A drain port 10 is fixedly installed on one side of the movable cover 15 corresponding to the inside of the rotating cylinder 3. The movable cover 15 can move upward under the elastic force of the spring 16, which facilitates lifting the filter cartridge 9 upward, thereby facilitating the removal of the filter cartridge 9 from the inside of the rotating cylinder 3 and facilitating subsequent cleaning of impurities inside the filter cartridge 9. The fixed frame 14 can limit the movable cover 15 and improve the stability of the movable cover 15.

[0023] In this embodiment, the positioning mechanism includes a fixing ring 19, which is fixedly disposed on the inner wall of the rotating cylinder 3. The fixing ring 19 has symmetrical insertion holes at both ends. Fixing blocks 17 are symmetrically fixedly disposed on the outer walls of both sides of the top of the filter cylinder 9. An insertion rod 18 is fixedly disposed at the bottom of the fixing block 17, and the bottom end of the insertion rod 18 is inserted into the insertion hole.

[0024] In this embodiment, the hydraulic rod 7 is fixedly mounted on the top of the L-shaped bracket 4. A mounting cover 6 is fixedly mounted on the bottom end of the output rod of the hydraulic rod 7. The upper cover 5 is rotatably limited and mounted on the bottom of the mounting cover 6. Limiting rods 8 are symmetrically fixed on both sides of the top of the mounting cover 6. The top ends of the limiting rods 8 are slidably mounted inside the L-shaped bracket 4. A servo motor 13 is fixedly mounted at the center of the inner top wall of the mounting cover 6. The output shaft of the servo motor 13 is fixedly connected to the top of the upper cover 5. A protrusion is fixedly mounted at the center of the bottom of the upper cover 5, and the protrusion is in contact with the top of the filter cartridge 9. A positioning rod 12 is symmetrically fixed at the bottom edge of the upper cover 5, corresponding to the outer side of the protrusion. The top of the rotating cylinder 3 is circularly provided with a positioning hole 11. The positioning rod 12 is inserted into the positioning hole 11. The limiting rod 8 can limit the mounting cover 6, improving the stability of the mounting cover 6. The mounting cover 6 and the upper cover 5 can be raised and lowered by the hydraulic rod 7, which facilitates the merging or separation of the upper cover 5 and the rotating cylinder 3. The positioning rod 12 and the positioning hole 11 facilitate the positioning of the upper cover 5. At the same time, the upper cover 5 can drive the rotating cylinder 3 to rotate, which facilitates the centrifugal separation of impurities.

[0025] When using this protein purification and washing device, the filter cartridge 9 is installed inside a rotating cylinder 3 away from the top cover 5 via a positioning mechanism, and the insertion rod 18 is inserted into the insertion hole. The insertion rod 18, the fixing ring 19, and the fixing block 17 facilitate the positioning of the filter cartridge 9. The protein raw material to be purified is added inside the filter cartridge 9. The rotating cylinder 3 and the rotating plate 2 are rotated, rotating the rotating cylinder 3 to the bottom of the top cover 5, and the other rotating cylinder 3 is away from the top cover 5. The hydraulic rod 7 drives the mounting cover 6 and the top cover 5 to descend. The top cover 5 drives the positioning rod 12 and inserts it into the positioning hole 11. The protrusion squeezes the filter cartridge 9. The filter cartridge 9 is compressed by the spring 16 through the movable cover 15, and the top of the rotating cylinder 3 is sealed by the top cover 5. The drain port 10 is located inside the filter cartridge 9. A valve is installed on the top. When the servo motor 13 is started, the servo motor 13 drives the top cover 5 to rotate. The top cover 5 drives the rotating cylinder 3 to rotate on the top of the rotating plate 2 through the positioning rod 12 and the positioning hole 11, which facilitates the centrifugation purification of protein raw materials. At the same time, the filter cylinder 9 and protein raw materials are placed in sequence inside the rotating cylinder 3 on the other side. After centrifugation, the hydraulic rod 7 drives the mounting cover 6 and the top cover 5 to rise, so that the rotating cylinder 3 is separated from the top cover 5. The movable cover 15 lifts the filter cylinder 9 upward under the elastic force of the spring 16, removes the filter cylinder 9 and filters the impurities inside it, which facilitates the cleaning of the filtered impurities. The rotating plate 2 can rotate the two rotating cylinders 3 and adjust them alternately, which can improve the efficiency of protein purification.

[0026] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A protein purification and washing device, comprising a base (1), characterized in that, A rotating plate (2) is provided at the top of one end of the base (1) for limiting rotation. A rotating cylinder (3) is symmetrically rotated on both sides of the top of the rotating plate (2). A filter cylinder (9) is installed inside the rotating cylinder (3) through a positioning mechanism. A lifting mechanism is provided below the filter cylinder (9) corresponding to the inside of the rotating cylinder (3). The lifting mechanism includes a fixed frame (14) and a movable cover (15). An L-shaped bracket (4) is fixedly provided at the top of the other end of the base (1). A top cover (5) is movably provided below the top of the L-shaped bracket (4) through a hydraulic rod (7). The top cover (5) is located above the rotating cylinder (3).

2. The protein purification and washing apparatus according to claim 1, characterized in that: The fixed frame (14) is fixedly installed at the bottom of the inner side of the rotating cylinder (3), and the movable cover (15) is movably installed on the outer side of the fixed frame (14). A spring (16) is fixedly installed between the inner top wall of the movable cover (15) and the bottom wall of the rotating cylinder (3).

3. The protein purification and washing apparatus according to claim 2, characterized in that: The filter cartridge (9) is placed on top of the movable cover (15) and in close contact with it. The spring (16) is set inside the fixed frame (14). A drain port (10) is fixedly provided on one side of the movable cover (15) corresponding to the inside of the rotating cylinder (3).

4. The protein purification and washing apparatus according to claim 1, characterized in that: The positioning mechanism includes a fixing ring (19), which is fixedly mounted on the inner wall of the rotating cylinder (3). The fixing ring (19) has symmetrical insertion holes at both ends.

5. The protein purification and washing apparatus according to claim 4, characterized in that: Fixing blocks (17) are symmetrically fixed on the outer walls of the top two sides of the filter cartridge (9). A plug rod (18) is fixedly installed at the bottom of the fixing block (17). The bottom end of the plug rod (18) is inserted into the inside of the plug hole.

6. The protein purification and washing apparatus according to claim 1, characterized in that: The hydraulic rod (7) is fixedly mounted on the top of the L-shaped bracket (4), and the bottom end of the output rod of the hydraulic rod (7) is fixedly mounted with a mounting cover (6). The upper cover (5) is rotatably limited and mounted at the bottom of the mounting cover (6).

7. The protein purification and washing apparatus according to claim 6, characterized in that: Limiting rods (8) are symmetrically fixed on both sides of the top of the mounting cover (6). The top of the limiting rods (8) is slidably set inside the L-shaped bracket (4). A servo motor (13) is fixedly installed at the center of the inner top wall of the mounting cover (6). The top of the output shaft cover (5) of the servo motor (13) is fixedly connected.

8. The protein purification and washing apparatus according to claim 7, characterized in that: A protrusion is fixedly provided at the bottom center of the top cover (5), and the protrusion is pressed against the top of the filter cylinder (9). A positioning rod (12) is symmetrically fixedly provided at the bottom edge of the top cover (5) corresponding to the outer side of the protrusion. The top of the rotating cylinder (3) is circularly provided with a positioning hole (11), and the positioning rod (12) is inserted into the positioning hole (11).

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