Microorganism sterile inoculation device

Abstract

A microorganism sterile inoculation device comprises a seed tank and an inoculation bottle, an inoculation pipeline is connected to the seed tank, and a silica gel hose is connected to a bottle opening of the inoculation bottle; the opening of the inoculation bottle faces downwards; the inoculation pipeline is connected with the silica gel hose through a primary-secondary joint, and a tank body control valve is arranged on the inoculation pipeline; a small discharge valve is arranged on the tank body control valve; the inoculation pipeline at the rear end of the tank body control valve is connected with a steam pipeline; a steam valve is arranged on the steam pipeline; one side of the silica gel hose communicates with an exhaust branch pipe, and a first water stop clamp is arranged on the outer side of the exhaust branch pipe; and a second water stop clamp is arranged on the outer side of the silica gel hose between the exhaust branch pipe and the inoculation bottle. According to the utility model, the inoculation device is disassembled and assembled through the primary and secondary joints, so that the risk of mixing of infectious microbes is reduced; high-temperature steam is used for sterilization, a small exhaust valve and an exhaust branch pipe are used as exhaust ports of the high-temperature steam, the high-temperature steam is guided to completely cover a to-be-sterilized area, the exhaust flow rate of the high-temperature steam can be controlled, and infectious microbe mixing is avoided.

Description

Technical Field

[0001] This utility model relates to the field of microbial inoculation technology, and in particular to a sterile microbial inoculation device. Background Technology

[0002] Fermentation inoculation devices are used in the microbial fermentation process to accurately and efficiently introduce seed culture or inoculum into the fermenter. Inoculation is a crucial process in bio-fermentation engineering technology, and it must be performed under aseptic conditions to prevent contamination. Currently available aseptic inoculation devices have several problems in use. First, existing aseptic inoculation devices primarily use flame sterilization, which poses significant safety risks during operation. Second, to prevent the entry of contaminants, existing devices mainly use a guiding method to introduce the seed culture or inoculum into the seed tank, which can easily result in some seed culture or inoculum residue, affecting the precise control of inoculation. Finally, the internal pipelines of the inoculation device are difficult to clean and disinfect, making it easy for contaminants to remain.

[0003] Therefore, in view of the shortcomings of the existing technology, it is necessary to design a sterile microbial inoculation device to solve the above problems.

[0004] It should be noted that the above introduction to the technical background is only for the purpose of providing a clear and complete explanation of the technical solution of this utility model and facilitating the understanding of those skilled in the art. It should not be assumed that the above content is known to those skilled in the art simply because it has been described in the background section of this utility model. Utility Model Content

[0005] To overcome the shortcomings of the prior art, the present invention discloses a microbial aseptic inoculation device to solve the problems of difficult sterilization operation and easy residue of seed liquid or strain in existing aseptic inoculation devices.

[0006] This utility model discloses a microbial aseptic inoculation device, including a seed tank for use as a fermentation container and an inoculation bottle for storing seed liquid or inoculum. An inoculation pipeline is connected to the seed tank, and a silicone tubing is connected to the mouth of the inoculation bottle. The mouth of the inoculation bottle is positioned downwards to allow the seed liquid or inoculum to flow out completely, avoiding residue. The inoculation pipeline and the silicone tubing are connected via a female-female connector. A tank control valve is provided on the inoculation pipeline. A small drain valve is provided on the tank control valve, connecting to the interior of the tank control valve. The inoculation pipeline at the rear end of the tank control valve is connected to... It has a steam pipeline that can supply high-temperature steam to the inoculation device for sterilization; the steam pipeline is equipped with a steam valve to control the flow rate of high-temperature steam; one side of the silicone tubing is connected to an exhaust branch pipe to provide a channel for the exhaust of high-temperature steam and to prevent external gases and bacteria from directly entering the silicone tubing; a first water-stop clamp is provided on the outside of the exhaust branch pipe to clamp the exhaust branch pipe and control the opening and closing of its internal pipeline; a second water-stop clamp is provided on the outside of the silicone tubing between the exhaust branch pipe and the inoculation bottle to seal the delivery channel between the exhaust branch pipe and the inoculation bottle.

[0007] Preferred technical solution: The connector includes a detachable first connector and a second connector. The first connector is connected to the inoculation tubing, and the second connector is connected to the silicone tubing, which facilitates the assembly and disassembly of the silicone tubing and the inoculation tubing.

[0008] Preferred technical solution: The inoculation pipeline and steam pipeline are made of stainless steel. The inoculation pipeline, tank control valve, small exhaust valve, steam pipeline, steam valve and first female connector are welded into an integral structure, which can reduce the risk of contamination by other bacteria.

[0009] Preferred technical solution: The silicone tubing and the exhaust branch pipe are integrally formed silicone tubing, the second female connector is fastened to the silicone tubing by the first hose clamp, and the silicone tubing is fastened to the mouth of the inoculation bottle by the second hose clamp, which can reduce the risk of contamination by other bacteria.

[0010] Preferred technical solution: A third water-stop clamp is provided on the silicone tubing between the exhaust branch pipe and the inoculation bottle to further reduce the risk of contamination by other microorganisms.

[0011] Preferred technical solution: The opening degree of the first water-stop clamp can be freely controlled, which facilitates the adjustment of the opening degree of the delivery pipe of the exhaust branch pipe.

[0012] Preferred technical solution: The inoculation device also includes a fixed hanger, and the bottom of the inoculation bottle is provided with a skirt structure. The inoculation bottle is detachably snapped onto the fixed hanger through the skirt structure.

[0013] Due to the application of the above technical solution, the beneficial effects of this utility model compared with the prior art are as follows:

[0014] This utility model discloses a microbial aseptic inoculation device. The device is assembled and disassembled using a female connector, which is convenient to operate and reduces the risk of contamination by other microorganisms. High-temperature steam is supplied to the inoculation device through a steam pipeline for sterilization. A small exhaust valve and an exhaust branch pipe are used as the outlet for the high-temperature steam. This not only guides the delivery of the high-temperature steam to fully cover the area to be sterilized, but also controls the flow rate of the high-temperature steam to prevent external microorganisms from being mixed into the inoculation device with the airflow. Attached Figure Description

[0015] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of a microbial aseptic inoculation device according to the present invention.

[0017] In the attached diagrams above, 1. Seed tank; 11. Inoculation pipeline; 12. Tank control valve; 13. Small exhaust valve; 14. Steam pipeline; 14a. Steam valve; 2. Inoculation bottle; 2a. Skirt structure; 21. Silicone hose; 21a. First hose clamp; 21b. Second hose clamp; 22. Exhaust branch pipe; 23. First water-stop clamp; 24. Second water-stop clamp; 25. Third water-stop clamp; 3. Female connector; 31. First female connector; 32. Second female connector; 4. Fixed hanger. Detailed Implementation

[0018] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0019] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be used interchangeably where appropriate for the description of embodiments of this application herein. Furthermore, the terms "comprising" and "having," and their synonyms, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0020] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing the present invention and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0021] Furthermore, in addition to indicating direction or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this utility model according to the specific circumstances.

[0022] Furthermore, the terms "installation," "setting," "equipped with," "connection," "linking," "fitting," and "fitting" should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Similarly, "fitting" can mean completely or partially fitted. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0023] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0024] Example:

[0025] like Figure 1 As shown, this utility model discloses a sterile microbial inoculation device, including a seed container 1, an inoculation bottle 2, and a connector 3. The main components of this utility model will be described in detail below:

[0026] like Figure 1 As shown, the seed tank 1 is connected to an inoculation pipeline 11, the inoculation pipeline 11 is equipped with a tank control valve 12, the tank control valve 12 is equipped with a small drain valve 13, the inoculation pipeline 11 at the rear end of the tank control valve 12 is connected to a steam pipeline 14, the steam pipeline 14 is connected to an external steam supply device, and the steam pipeline 14 is equipped with a steam valve 14a.

[0027] like Figure 1As shown, the inoculation bottle 2 has its mouth facing downwards and is connected to a silicone tube 21. One side of the silicone tube 21 is connected to an exhaust branch pipe 22. A first water-stop clamp 23 is provided on the outside of the exhaust branch pipe 22, and the opening of the first water-stop clamp 23 can be freely controlled. A second water-stop clamp 24 is provided on the outside of the silicone tube 21 between the exhaust branch pipe 22 and the inoculation bottle 2.

[0028] like Figure 1 As shown, the female connector 3 includes a first female connector 31 and a second female connector 32 that are detachably connected. The first female connector 31 is connected to the inoculation tubing 11, and the second female connector 32 is connected to the silicone tubing 21.

[0029] The method and principle of this utility model are as follows: During use, keep the tank control valve 12 and the small drain valve 13 closed, and the first and second water-stop clamps 23 and 24 clamped. Connect the first and second female connectors 31 and 32 to connect the inoculation pipeline 11 to the silicone tubing 21. Open the steam valve 14a to allow high-temperature steam to enter the inoculation device through the steam pipeline 14. Adjust the opening of the small drain valve 13 and the first water-stop clamp 23 to allow high-temperature steam to flow out from the adjusting small drain valve 13 and the exhaust branch pipe 22. At this time, the high-temperature steam thoroughly sterilizes the inoculation pipeline 11 at the rear end of the tank control valve 12 and the silicone tubing 21 at the front end of the second water-stop clamp 24. During this process, as long as the high-temperature steam continues to flow out from the adjusting small drain valve 13 and the exhaust branch pipe 22, external air and bacteria cannot enter the inoculation device through the small drain valve 13 and the exhaust branch pipe 22. Maintain this state for 30 minutes to ensure thorough sterilization; then, sequentially close the small drain valve 13 and the steam valve 14a, and open the tank control valve 12 to connect the inoculation pipeline 11 to the seed tank 1. After five minutes, close the first stop clamp 23, at which point the internal pipelines of the inoculation device will have cooled down considerably; then, shake the inoculation bottle 2 to mix the microbial solution evenly, open the second stop clamp 24, and allow the microbial solution to flow sequentially through the silicone tubing 21 and the inoculation pipeline 11 into the seed tank 1. Once all the microbial solution has entered the seed tank 1, the inoculation is complete, and the seed tank 1 will begin cultivation; subsequently, close the tank control valve 12 and the second stop clamp 24, and open the first stop clamp 23, the small drain valve 13, and the steam valve 14a for sterilization for 10 minutes; finally, close the steam valve 14a, the first stop clamp 23, and the small drain valve 1, and disassemble the first female connector 31 and the second female connector 32, wrapping them separately with sterile gauze to prevent contamination.

[0030] like Figure 1 As shown, to prevent contamination by miscellaneous bacteria, the inoculation pipeline 11 and the steam pipeline 14 are made of stainless steel. The inoculation pipeline 11, the tank control valve 12, the small exhaust valve 13, the steam pipeline 14, the steam valve 14a, and the first female connector 31 are welded to form an integral structure, reducing connection breaks and lowering the possibility of contamination.

[0031] like Figure 1 As shown, to further prevent contamination by miscellaneous bacteria, the silicone hose 21 and the exhaust branch pipe 22 are integrally formed silicone tubes. The second female connector 32 is fastened to the silicone hose 21 by the first hose clamp 21a, and the silicone hose 21 is fastened to the mouth of the inoculation bottle 2 by the second hose clamp 21b, ensuring the sealing of the connection end and further reducing the possibility of contamination.

[0032] like Figure 1 As shown, in order to further reduce contamination by miscellaneous bacteria, a third water-stop clamp 25 is also provided on the silicone hose 21 between the exhaust branch pipe 22 and the inoculation bottle 2. This clamp, in conjunction with the second water-stop clamp 24, provides double restriction on the liquid flow within the silicone hose 21.

[0033] like Figure 1 As shown, to facilitate the operation of the inoculation device, the inoculation device also includes a fixed hanger 4. The bottom of the inoculation bottle 2 is provided with a skirt structure 2a. The inoculation bottle 2 is detachably attached to the fixed hanger 4 through the skirt structure 2a, making it easier to pick up and put down the inoculation bottle 2 and reducing the difficulty of operation.

[0034] Finally, it should be noted that the above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A sterile microbial inoculation device, comprising a seed container (1) and an inoculation bottle (2), wherein an inoculation tube (11) is connected to the seed container (1), and a silicone tubing (21) is connected to the mouth of the inoculation bottle (2), characterized in that: The inoculation bottle (2) is positioned with its mouth facing downwards. The inoculation pipeline (11) and the silicone hose (21) are connected by a female connector (3). The inoculation pipeline (11) is equipped with a tank control valve (12), and the tank control valve (12) is equipped with a small exhaust valve (13). A steam pipeline (14) is connected to the inoculation pipeline (11) at the rear end of the tank control valve (12). A steam valve (14a) is provided on the steam pipeline (14). An exhaust branch pipe (22) is connected to one side of the silicone hose (21). A first water-stop clamp (23) is provided on the outside of the exhaust branch pipe (22). A second water-stop clamp (24) is provided on the outside of the silicone hose (21) between the exhaust branch pipe (22) and the inoculation bottle (2).

2. The microbial aseptic inoculation device according to claim 1, characterized in that: The female connector (3) includes a first female connector (31) and a second female connector (32) that are detachably connected. The first female connector (31) is connected to the inoculation tubing (11), and the second female connector (32) is connected to the silicone tubing (21).

3. The aseptic microbial inoculation device according to claim 2, characterized in that: The inoculation pipeline (11) and steam pipeline (14) are stainless steel pipelines. The inoculation pipeline (11), tank control valve (12), small exhaust valve (13), steam pipeline (14), steam valve (14a) and first female connector (31) are welded together to form an integral structure.

4. The microbial aseptic inoculation device according to claim 2, characterized in that: The silicone hose (21) and the exhaust branch pipe (22) are integrally formed silicone tubes. The second female connector (32) is fastened to the silicone hose (21) by the first hose clamp (21a). The silicone hose (21) is fastened to the mouth of the inoculation bottle (2) by the second hose clamp (21b).

5. The aseptic microbial inoculation device according to claim 4, characterized in that: A third water-stop clamp (25) is provided on the silicone hose (21) between the exhaust branch pipe (22) and the inoculation bottle (2).

6. The microbial aseptic inoculation device according to claim 1, characterized in that: The opening degree of the first water-stopping clamp (23) can be freely controlled.

7. The microbial aseptic inoculation device according to claim 1, characterized in that: The inoculation device also includes a fixed hanger (4), and the bottom of the inoculation bottle (2) is provided with a skirt structure (2a). The inoculation bottle (2) is detachably snapped onto the fixed hanger (4) through the skirt structure (2a).

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