Liquid closed conversion piece and culture device

Abstract

The utility model discloses a liquid sealing conversion piece and a culture device, and the liquid sealing conversion piece comprises a conversion joint which comprises a cover body, a plugging connecting piece and a first joint; the cover body is provided with a first hollow hole, the plugging connecting piece is inserted in the first hollow hole and is in sealed connection with the cover body, the plugging connecting piece is provided with a second hollow hole, and the first joint is inserted in the second hollow hole and is in sealed connection with the plugging connecting piece; the second connector is used for being connected with the first connector in an inserted mode to form a sealing structure. The utility model provides a liquid closed conversion piece and a culture device, which can reduce the time for liquid transfer and breathable culture conversion of a cell culture container, effectively reduce the probability of pollution in the conversion process and ensure the connection leakproofness.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a liquid-sealed conversion component and culture device. Background Technology

[0002] The production process of biopharmaceuticals requires cell culture and amplification. First, cells are cultured in culture dishes. Once the cells reach a certain multiple, they are transferred to cell culture containers for further amplification, including aseptic transfer of cell suspension and rapid static aeration culture.

[0003] Currently, a threaded connection is made to the opening of the cell culture container, and a knob cap is connected to the liquid storage container. The knob cap is threaded to the opening of the container, so that the liquid storage container can transfer liquid to the cell culture container. After the transfer is completed, the knob cap needs to be unscrewed and then the vent cap needs to be screwed on before aeration culture can be carried out. The cell culture container takes a long time to switch between liquid transfer and aeration culture, and contamination is easily generated in the process. Utility Model Content

[0004] The purpose of this invention is to provide a liquid-sealed conversion component and culture device that can reduce the time required for liquid transfer and aeration conversion of cell culture containers, effectively reduce the probability of contamination during the conversion process, and ensure the airtightness of the connection.

[0005] To achieve the above objectives, this utility model provides a liquid-sealed conversion component, comprising:

[0006] An adapter, the adapter comprising a cover, a plug-in connector, and a first connector;

[0007] The cover has a first hollow hole, the plug-in connector is inserted into the first hollow hole and is sealed to the cover, the plug-in connector has a second hollow hole, the first connector is inserted into the second hollow hole and is sealed to the plug-in connector.

[0008] The second connector is used to insert into the first connector and form a sealing structure.

[0009] Preferably, the first hollow hole includes a locking groove and a positioning groove that are connected and coaxial from bottom to top. The bottom of the locking groove penetrates the bottom wall of the cover, and the opening of the locking groove is connected to the bottom of the positioning groove. The diameter of the locking groove is larger than the diameter of the positioning groove.

[0010] The plug-in connector includes a hollow tube and a first hollow boss connected and coaxially from bottom to top. The top of the hollow tube is connected to the bottom of the first hollow boss. The hollow hole of the hollow tube and the hollow hole of the first hollow boss are connected to form a second hollow hole. A ring of protrusions is provided around the outer periphery of the hollow tube near the top. The outer diameter of the protrusions is larger than the diameter of the positioning groove. The first hollow boss is embedded in the positioning groove, and the protrusions are located at the top of the locking groove.

[0011] Preferably, the first hollow hole further includes a skirt groove disposed above the positioning groove and coaxial with and connected to the positioning groove, the opening of the positioning groove being connected to the bottom of the skirt groove, and the opening of the skirt groove penetrating the top wall of the cover; the diameter of the positioning groove is smaller than the diameter of the skirt groove;

[0012] The first hollow protrusion has a skirt around its outer periphery near the top; the outer diameter of the skirt is the same as the diameter of the skirt groove, the distance between the bottom of the skirt and the top of the protrusion is the same as the depth of the positioning groove, and the skirt is embedded in the skirt groove.

[0013] Preferably, the plug-in connector further includes a second hollow boss, the second hollow boss, the first hollow boss and the hollow tube are coaxial, the bottom of the second hollow boss is connected to the top of the first hollow boss; the hollow hole of the hollow tube, the hollow hole of the first hollow boss and the hollow hole of the second hollow boss are connected to form the second hollow hole.

[0014] Preferably, the hollow hole of the second hollow boss includes a lower hollow hole and an upper hollow hole that are connected and coaxial from bottom to top. The bottom of the lower hollow hole is connected to the top of the hollow hole of the first hollow boss, the top of the lower hollow hole is connected to the bottom of the upper hollow hole, and the top of the upper hollow hole penetrates the top wall of the second hollow boss.

[0015] The diameter of the upper hollow hole gradually increases from bottom to top.

[0016] Preferably, when the first connector is a male connector, the second connector is a female connector; or when the first connector is a female connector, the second connector is a male connector.

[0017] Preferably, the second connector has a insertion groove, which extends from one side of the outer peripheral wall of the second connector and through the other side of the outer peripheral wall of the second connector in the same horizontal direction; a insertion piece is inserted into the insertion groove, the insertion piece includes an insertion part and a limiting part, the limiting part and the insertion part are vertically connected, the limiting part is inserted into the insertion groove, and a limiting hole is formed on the limiting part, the semicircular diameter of the limiting hole near the insertion part is larger than the semicircular diameter of the limiting hole away from the insertion part;

[0018] The first connector has a limiting groove around its outer periphery, and the inner diameter of the limiting groove is the same as the semicircular diameter of the limiting hole away from the insertion part.

[0019] Preferably, the first connector has a insertion groove, which extends from one side of the outer peripheral wall of the first connector and through the other side of the outer peripheral wall of the first connector in the same horizontal direction; an insertion piece is inserted into the insertion groove, the insertion piece includes an insertion part and a limiting part, the limiting part and the insertion part are vertically connected, the limiting part is inserted into the insertion groove, and a limiting hole is formed on the limiting part, the semicircular diameter of the limiting hole near the insertion part is larger than the semicircular diameter of the limiting hole away from the insertion part;

[0020] The second connector has a limiting groove around its outer periphery, and the inner diameter of the limiting groove is the same as the semicircular diameter of the limiting hole away from the insertion part.

[0021] This utility model provides a liquid-sealed culture device, comprising:

[0022] Liquid-sealed conversion components as described above;

[0023] A cell culture container, wherein the adapter is installed at the container opening of the cell culture container;

[0024] A liquid storage container for storing cell suspension, the liquid storage container being connected to the second connector;

[0025] A venting element for supplying oxygen to the cell culture container, the venting element being connected to the second connector.

[0026] Preferably, it further includes a connecting plug for insertion with the first connector.

[0027] Compared with the prior art, the liquid-sealed conversion component and culture device of this utility model have the following advantages: the container mouth of the cell culture container can be equipped with a conversion connector, the liquid storage container and the vent can be connected to the second connector, and the insertion of the first connector and different second connectors of the conversion connector can quickly realize the conversion between the cell culture container and the liquid storage container and the vent, reducing the time for liquid transfer and vent culture conversion of the cell culture container, and effectively reducing the probability of contamination during the conversion process.

[0028] The plug-in connector in the adapter is inserted into the first hollow hole of the cover, and the first connector is inserted into the second hollow hole of the plug-in connector to achieve a sealed connection between the first connector and the cover. The first connector and the second connector are plugged in to form a sealed structure, which can ensure the airtightness of the entire adapter connection. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the structure of the liquid-sealed conversion component described in an embodiment of the present invention;

[0030] Figure 2 This is a schematic diagram of the structure of the liquid-sealed conversion component according to another embodiment of the present invention;

[0031] Figure 3 This is a schematic diagram of the structure of the cover body according to an embodiment of the present utility model;

[0032] Figure 4 This is a schematic diagram of the structure of the plug-in connector described in an embodiment of the present utility model;

[0033] Figure 5 This is a schematic diagram of the structure of the plug-in connector as described in this embodiment of the present invention being inserted into the cover body;

[0034] Figure 6 This is a schematic diagram of the limiting groove where the plug-in tab is not inserted into the second connector according to an embodiment of the present invention;

[0035] Figure 7 This is a schematic diagram of the structure by which the plug-in piece limits the first connector when it is inserted into the limiting groove of the second connector, as described in this embodiment of the utility model.

[0036] Figure 8 This is a schematic diagram of the structure of the present invention, which shows that the first connector is not limited when the plug-in piece is inserted into the limiting groove of the second connector;

[0037] Figure 9 This is a schematic diagram of the structure of the liquid-sealed conversion component according to another embodiment of the present utility model;

[0038] Figure 10 This is a schematic diagram of the liquid-sealed culture device described in this embodiment of the present invention during liquid transfer;

[0039] Figure 11 This is a schematic diagram of the structure of the liquid-sealed culture device described in this embodiment of the invention during aeration culture.

[0040] Figure 12 This is a schematic diagram of the structure of the second connector connecting the liquid storage container to the liquid-sealed culture device described in this embodiment of the present invention;

[0041] Figure 13 This is a schematic diagram of the structure of the second connector of the liquid closed culture device with the ventilated component pulled out, as described in this embodiment of the utility model.

[0042] Figure 14 This is an exploded view of the venting component, the second connecting pipe, and the second connector described in the embodiment of this utility model;

[0043] Figure 15 This is a schematic diagram of the connection between the second connector and the adapter connector of the ventilated component according to an embodiment of the present invention.

[0044] Figure 16 This is a schematic diagram of the structure of the second connector pull-out conversion connector connected to the ventilated component according to an embodiment of the present invention;

[0045] Figure 17 This is an exploded view of the adapter and connecting plug described in this embodiment of the utility model;

[0046] Figure 18 This is a schematic diagram of the structure of the adapter and connecting plug as described in the embodiment of this utility model;

[0047] Figure 19 This is a schematic diagram of the structure of the connecting plug pull-out adapter according to an embodiment of the present utility model;

[0048] Figure 20 This is an exploded view of the adapter and connecting plug according to another embodiment of the present invention;

[0049] Figure 21 This is a schematic diagram of the structure of the adapter and connecting plug as described in another embodiment of the present invention;

[0050] Figure 22 This is a schematic diagram of the connection plug pull-out adapter according to another embodiment of the present invention;

[0051] Figure 23 This is a cross-sectional structural diagram of the adapter and connecting plug described in this embodiment of the utility model;

[0052] Figure 24 This is a cross-sectional structural diagram of the adapter and connecting plug as described in this embodiment of the utility model;

[0053] Figure 25 This is a cross-sectional structural diagram of the adapter and connecting plug according to another embodiment of the present invention;

[0054] Figure 26 This is a schematic diagram of the structure of the adapter and connecting plug as described in another embodiment of the present invention;

[0055] In the diagram, 1 is the liquid storage container; 2 is the venting component; 3 is the cell culture container; 31 is the container opening; 4 is the adapter; 41 is the cover; 411 is the first hollow hole; 4111 is the locking groove; 4112 is the positioning groove; 4113 is the skirt groove; 42 is the plug-in connector; 421 is the second hollow hole; 422 is the hollow tube; 423 is the protrusion; 424 is the first hollow boss; 425 is the skirt; 426 is the second hollow boss; 427 is the upper hollow hole; 428 is the lower hollow hole; 43 is the first connector; 5 is the second connector; 6 is the needle filter; 7 is the connecting plug; 8 is the first connecting tube; 9 is the second connecting tube; 10 is the sealing ring; 11 is the plug-in groove; 12 is the plug-in piece; 121 is the limiting part; 1211 is the limiting hole; 122 is the plug-in part; and 13 is the limiting groove. Detailed Implementation

[0056] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit its scope.

[0057] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In the description of the embodiments of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this utility model based on the specific circumstances.

[0058] In the description of this utility model, it should be understood that the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," "longitudinal," "X-axis direction," "Y-axis direction," and "Z-axis direction," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, some of the above terms, in addition to indicating orientation or positional relationships, may also be used to indicate other meanings. For example, the term "upper" may, in some cases, also indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this application according to the specific circumstances.

[0059] like Figure 1-5 As shown, a liquid-sealed conversion component according to an embodiment of the present invention includes: a conversion connector 4 and a second connector 5;

[0060] The adapter 4 includes a cover 41, a plug-in connector 42, and a first connector 43;

[0061] The cover 41 has a first hollow hole 411, the plug-in connector 42 is inserted into the first hollow hole 411 and is sealed to the cover 41, the plug-in connector 42 has a second hollow hole 421, the first connector 43 is inserted into the second hollow hole 421 and is sealed to the plug-in connector 42.

[0062] The second connector 5 is used to connect with the first connector 43 to form a sealing structure.

[0063] It should be noted that, as Figure 10-13 As shown, the container opening 31 of the cell culture container 3 can be fitted with a conversion connector 4, and the liquid storage container 1 and the venting component 2 can be connected to the second connector 5. The insertion of the first connector 43 of the conversion connector 4 and the different second connectors 5 can quickly realize the conversion between the cell culture container 3 and the liquid storage container 1 and the venting component 2, reducing the time for liquid transfer and aeration culture conversion of the cell culture container 3, and effectively reducing the probability of contamination during the conversion process.

[0064] The plug-in connector 42 in the adapter 4 is inserted into the first hollow hole 411 of the cover 41, and the first connector 43 is inserted into the second hollow hole 421 of the plug-in connector 42, so as to achieve a sealed connection between the first connector 43 and the cover 41. The first connector 43 and the second connector 5 are inserted to form a sealed structure, which can ensure the airtightness of the entire adapter connection.

[0065] The cell culture container 3 can be a cell factory or a culture flask.

[0066] The cover 41 is preferably made of HDPE high-density polyethylene, and the roundness deformation after injection molding is less than 0.1mm.

[0067] The material of the plug-in connector 42 is preferably medical-grade silicone. Medical-grade silicone has a certain degree of toughness and shrinkage, which can withstand the impact force of plugging and unplugging the first connector 43 and the second connector 5. Because medical-grade silicone is relatively soft, the first connector 43 is not easily damaged when inserted.

[0068] like Figure 3-5 As shown, in a more specific embodiment, the first hollow hole 411 includes a locking groove 4111 and a positioning groove 4112 that are connected and coaxial from bottom to top. The bottom of the locking groove 4111 penetrates the bottom wall of the cover 41, and the opening of the locking groove 4111 connects to the bottom of the positioning groove 4112. The diameter of the locking groove 4111 is larger than the diameter of the positioning groove 4112.

[0069] The plug-in connector 42 includes a hollow tube 422 and a first hollow boss 424 connected and coaxially from bottom to top. The top of the hollow tube 422 is connected to the bottom of the first hollow boss 424. The hollow hole of the hollow tube 422 and the hollow hole of the first hollow boss 424 are connected to form a second hollow hole 421. A protrusion 423 is provided around the outer periphery of the hollow tube 422 near the top. The outer diameter of the protrusion 423 is larger than the diameter of the positioning groove 4112. The first hollow boss 424 is embedded in the positioning groove 4112, and the protrusion 423 is located at the top of the locking groove 4111.

[0070] It should be noted that the outer diameter of the protrusion 423 is larger than the diameter of the positioning groove 4112. The top of the protrusion 423 abuts against the bottom wall of the top of the cover 41, which improves the sealing between the plug-in connector 42 and the cover 41. It also requires a greater force to pull the plug-in connector 42 out of the cover 41, preventing the plug-in connector 42 from easily coming off the cover 41. The connection between the plug-in connector 42 and the cover 41 is tighter and more stable.

[0071] like Figure 3-5 As shown, in a more specific embodiment, the first hollow hole 411 further includes a skirt groove 4113 disposed above the positioning groove 4112 and coaxial with and connected to the positioning groove 4112. The opening of the positioning groove 4112 is connected to the bottom of the skirt groove 4113, and the opening of the skirt groove 4113 penetrates the top wall of the cover 41. The diameter of the positioning groove 4112 is smaller than the diameter of the skirt groove 4113.

[0072] The first hollow boss 424 has a skirt 425 around its outer periphery near the top; the outer diameter of the skirt 425 is the same as the diameter of the skirt groove 4113, the distance between the bottom of the skirt 425 and the top of the protrusion 423 is the same as the depth of the positioning groove 4112, and the skirt 425 is embedded in the skirt groove 4113.

[0073] It should be noted that the diameter of the locking groove 4111 is larger than the diameter of the positioning groove 4112, and the diameter of the positioning groove 4112 is smaller than the diameter of the skirt groove 4113. Thus, when the plug-in connector 42 is forcefully inserted downward into the first hollow hole 411 of the cover 41, the skirt 425 arranged along the outer periphery near the top of the first hollow boss 424 is limited by the top of the positioning groove 4112, and the bottom of the skirt 425 is locked in the skirt groove 4113 to achieve positioning.

[0074] Since the distance between the bottom of the skirt 425 and the top of the protrusion 423 is the same as the depth of the positioning groove 4112, the skirt 425 is embedded in the skirt groove 4113. Therefore, the platform formed between the positioning groove 4112 and the skirt groove 4113 is stuck between the bottom of the skirt 425 and the top of the protrusion 423, ensuring that the position of the plug-in connector 42 is stable in the cover 41.

[0075] like Figure 4-5 As shown, in a more specific embodiment, the plug-in connector 42 further includes a second hollow boss 426, the second hollow boss 426, the first hollow boss 424 and the hollow tube 422 are coaxial, the bottom of the second hollow boss 426 is connected to the top of the first hollow boss 424; the hollow hole of the hollow tube 422, the hollow hole of the first hollow boss 424 and the hollow hole of the second hollow boss 426 are connected to form a second hollow hole 421.

[0076] It should be noted that the plug-in connector 42 also includes a second hollow boss 426. The bottom of the second hollow boss 426 is connected to the top of the first hollow boss 424. The second hollow boss 426 can increase the rigidity of the plug-in connector 42, making it easier to plug into the first connector 43.

[0077] like Figure 4 As shown, in a more specific embodiment, the hollow hole of the second hollow boss 426 includes a lower hollow hole 428 and an upper hollow hole 427 that are connected and coaxial from bottom to top. The bottom of the lower hollow hole 428 is connected to the top of the hollow hole of the first hollow boss 424, the top of the lower hollow hole 428 is connected to the bottom of the upper hollow hole 427, and the top of the upper hollow hole 427 penetrates the top wall of the second hollow boss 426.

[0078] The diameter of the upper hollow hole 427 gradually increases from bottom to top.

[0079] It should be noted that the hollow hole of the second hollow boss 426 is divided into an upper hollow hole 427 and a lower hollow hole 428. The diameter of the upper hollow hole 427 gradually increases from bottom to top, which serves as a directional guide and facilitates the insertion of the first connector 43 into the plug-in connector 42 through the upper hollow hole 427.

[0080] In a more specific embodiment, when the first connector 43 is a male connector, the second connector 5 is a female connector; or when the first connector 43 is a female connector, the second connector 5 is a male connector.

[0081] It should be noted that the connection between the male and female connectors is simpler in structure. The male connector is an MPC male connector, and the female connector is an MPC female connector, where MPC stands for methyl phenylcarbamate.

[0082] like Figure 1 , 6As shown in Figure 8, in a more specific embodiment, the second connector 5 is provided with a insertion groove 11, which extends from one side of the outer peripheral wall of the second connector 5 and through the other side of the outer peripheral wall of the second connector 5 in the same horizontal direction; a insertion piece 12 is inserted into the insertion groove 11, the insertion piece 12 includes an insertion part 122 and a limiting part 121, the limiting part 121 and the insertion part 122 are vertically connected, the limiting part 121 is inserted into the insertion groove 11, and a limiting hole 1211 is provided on the limiting part 121, the semicircular diameter of the limiting hole 1211 near the insertion part 122 is larger than the semicircular diameter of the limiting hole 1211 away from the insertion part 122;

[0083] The first connector 43 is provided with a limiting groove 13 around its outer periphery. The inner diameter of the limiting groove 13 is the same as the semi-circular diameter of the limiting hole 1211 away from the insertion part 122.

[0084] It should be noted that the second connector 5 is a female connector, and the first connector 43 is a male connector.

[0085] like Figure 2 As shown, in a more specific embodiment, the first connector 43 is provided with a insertion groove 11, which extends from one side of the outer peripheral wall of the first connector 43 and through the other side of the outer peripheral wall of the first connector 43 in the same horizontal direction; a insertion piece 12 is inserted into the insertion groove 11, the insertion piece 12 includes an insertion part 122 and a limiting part 121, the limiting part 121 and the insertion part 122 are vertically connected, the limiting part 121 is inserted into the insertion groove 11, and a limiting hole 1211 is provided on the limiting part 121, the semicircular diameter of the limiting hole 1211 near the insertion part 122 is larger than the semicircular diameter of the limiting hole 1211 away from the insertion part 122;

[0086] The second connector 5 is provided with a limiting groove 13 around its outer periphery. The inner diameter of the limiting groove 13 is the same as the semi-circular diameter of the limiting hole 1211 away from the insertion part 122.

[0087] It should be noted that the first connector 43 is a female connector, and the second connector 5 is a male connector.

[0088] During the insertion of the male and female connectors, the limiting part 121 of the insertion / removal piece 12 is inserted into the insertion / removal groove 11. The limiting hole 1211 on the limiting part 121 is connected to the interior of the female connector. The insertion / removal piece 122 is pressed down, and at this time the limiting part 121 is inserted to the deepest part of the insertion / removal groove 11. Since the semi-circular diameter of the limiting hole 1211 near the insertion / removal piece 122 is larger than the diameter of the limiting groove 13, the male connector can pass smoothly through the limiting hole 1211 when it is inserted into the female connector.

[0089] When it is necessary to limit the male connector, pull the insertion part 122 of the insertion piece 12 outward, so that the limiting part 121 moves outward until the limiting hole 1211 moves away from the semicircle of the insertion part 122 and is inserted into the limiting groove 13 of the male connector. Only then will the limiting part 121 stop moving, thus limiting the male connector and preventing the male connector and female connector from being separated.

[0090] When it is necessary to separate the male and female connectors, press the insertion part 122 of the insertion and extraction piece 12. The semicircle of the limiting hole 1211 moves away from the insertion and extraction part 122 and leaves the limiting groove 13, so that the male and female connectors can be separated.

[0091] In a more specific embodiment, a spring is provided on the inner side of the insertion slot 11 away from the insertion part 122. When the insertion part 122 is pressed and the limiting part 121 is inserted into the insertion slot 11, the spring is compressed. When the insertion part 122 is released, the spring returns to its original position and pushes the limiting part 121 and the insertion part 122 outward, so that the semicircle of the limiting hole 1211 away from the insertion part 122 can be inserted into the limiting groove 13.

[0092] like Figure 9 As shown, in a more specific embodiment, a pluggable connector 42 is provided on the outer side of the second connector 5, and a cover 41 is provided on the outer side of the pluggable connector 42, so that the adapter 4 can be installed on the container opening of one device, and the cover 41 connected to the second connector 5 can also be installed on the container opening of another device. When the first connector 43 and the second connector 5 are plugged in, the connection between the two devices is realized.

[0093] like Figure 3-5 As shown, in a more specific embodiment, the thickness of the sidewall of the cover 41 is 2.8 mm, the depth of the positioning groove 4112 is 2 mm, and the depth of the skirt groove 4113 is 0.7 mm. The cover 41 can effectively withstand the external force borne by the insertion and removal of the plug-in connector 42 or the first connector 43.

[0094] The outer diameter of the first hollow boss 424 is 1.4 mm smaller than the diameter of the positioning groove 4112, that is, the gap between the first hollow boss 424 and the positioning groove 4112 is 0.7 mm. The inner diameter of the skirt 425 is 2.4 mm larger than the outer diameter of the first hollow boss 424, that is, the skirt 425 is hollowed out by 1.2 mm. The skirt 425 of the plug-in connector 42 can be effectively contracted. The thickness of the skirt 425 is 0.7 mm. The skirt 425 can be effectively locked in the skirt groove 4113 of the cover 41.

[0095] The second hollow boss 426 has a height of 5mm, which makes the plug-in connector 42 protrude a certain height from the upper surface of the cover 41 after being inserted into the cover 41. This can increase the rigidity of the plug-in connector 42 and facilitate docking with the first connector 43.

[0096] The plug-in connector 42 and the first connector 43 are interference-fitted by 0.1mm, which can effectively increase the toughness of the plug-in connector 42 and prevent leakage.

[0097] like Figure 10-16 As shown, a liquid-sealed culture device according to an embodiment of the present invention includes: a liquid-sealed conversion component, a cell culture container 3, a liquid storage container 1, and a venting component 2 as described above.

[0098] The container opening 31 of the cell culture container 3 is equipped with an adapter 4;

[0099] Liquid storage container 1 is used to store cell suspension, and liquid storage container 1 is connected to a second connector 5;

[0100] Vent 2, vent 2 is used to supply oxygen to cell culture container 3, and vent 2 is connected to a second connector 5;

[0101] It should be noted that the liquid storage container 1 is connected to one end of the first connecting pipe 8, and the other end of the first connecting pipe 8 is connected to the second connector 5. When transferring liquid, the second connector 5 connected to the liquid storage container 1 and the conversion connector 4 are plugged in.

[0102] The venting component 2 is connected to one end of the second connecting tube 9, and the other end of the second connecting tube 9 is connected to another second connector 5. When ventilating for culture, the second connector 5 connected to the venting component 2 and the conversion connector 4 are plugged in.

[0103] Both the first connecting pipe 8 and the second connecting pipe 9 are preferably thermoplastic pipes, which are made of 3 / 8*5 / 8 TPE thermoplastic elastomer material.

[0104] The venting component 2 is preferably a needle filter 6, which can filter bacteria in the air. The filtered air then enters the container opening 31 of the cell culture container 3 through the second connector 5 and the conversion connector 4 to provide oxygen for cell culture. The needle filter 6 is preferably a 0.22µm needle filter, and the material of the needle filter 6 is preferably PTFE (polytetrafluoroethylene).

[0105] like Figure 17-26 As shown, in a more specific embodiment, a connecting plug 7 is also included, which is used to connect with the first connector 43.

[0106] It should be noted that when liquid transfer or aeration culture is not required, the connecting plug 7 and the first connector 43 are plugged in to seal the entire cell culture container 3.

[0107] like Figure 17-19 As shown in Figures 25-26, in a more specific embodiment, when the first connector 43 is a male connector, the connecting plug 7 is a female plug;

[0108] The connecting plug 7 has a insertion groove 11, which extends horizontally from one side of the outer peripheral wall of the connecting plug 7 to the other side. An insertion piece 12 is inserted into the insertion groove 11. The insertion piece 12 includes an insertion portion 122 and a limiting portion 121, which are vertically connected. The limiting portion 121 is inserted into the insertion groove 11, and a limiting hole is formed on the limiting portion 121. The semi-circular diameter of the limiting hole near the insertion portion 122 is larger than the semi-circular diameter of the limiting hole away from the insertion portion 122. The first connector 43 has a ring of limiting grooves 13 along its outer periphery. The inner diameter of the limiting grooves 13 is the same as the semi-circular diameter of the limiting hole away from the insertion portion 122.

[0109] like Figure 20-24 As shown, in a more specific embodiment, when the first connector 43 is a female connector, the connecting plug 7 is a male plug;

[0110] The first connector 43 has a insertion groove 11, which extends horizontally from one side of the outer peripheral wall of the first connector 43 to the other side. An insertion piece 12 is inserted into the insertion groove 11. The insertion piece 12 includes an insertion portion 122 and a limiting portion 121, which are vertically connected. The limiting portion 121 is inserted into the insertion groove 11, and a limiting hole is formed on the limiting portion 121. The semi-circular diameter of the limiting hole near the insertion portion 122 is larger than the semi-circular diameter away from the insertion portion 122. The connecting plug 7 has a ring of limiting grooves 13 along its outer periphery. The inner diameter of the limiting grooves 13 is the same as the semi-circular diameter of the limiting hole away from the insertion portion 122.

[0111] It should be noted that the male connector is an MPC male connector, the female connector is an MPC female connector, the male plug is an MPC male plug, the female plug is an MPC female plug, and MPC stands for methyl phenylcarbamate.

[0112] like Figure 17 As shown, in a more specific embodiment, when the first connector 43 is a male connector and the connecting plug 7 is a female plug, a sealing ring 10 is provided on the surface of the first connector 43 near the connecting plug 7.

[0113] like Figure 20 As shown, in a more specific embodiment, when the first connector 43 is a female connector and the connecting plug 7 is a male plug, a sealing ring 10 is provided on the surface of the connecting plug 7 near the first connector 43.

[0114] It should be noted that the sealing ring 10 can improve the sealing performance of the connection, and the sealing ring 10 is preferably made of medical-grade silicone.

[0115] In a more specific embodiment, when the first connector 43 is a male connector and the second connector 5 is a female connector, a sealing ring 10 is provided on the surface of the first connector 43 near the second connector 5; when the first connector 43 is a female connector and the second connector 5 is a male connector, a sealing ring 10 is provided on the surface of the second connector 5 near the first connector 43, thereby improving the sealing performance of the connection between the first connector 43 and the second connector 5.

[0116] like Figure 3-5 As shown in Figures 1 and 12, in a more specific embodiment, the outer peripheral side of the container opening 31 is provided with external threads, and the inner peripheral side of the cover 41 is provided with internal threads. The cover 41 is installed at the container opening 31 of the cell culture container 3 by means of threads.

[0117] It should be noted that the tightness of the container opening 31 and the cover 41 is achieved by the threaded installation, which can better prevent bacteria from entering the cell culture container 3 through the gap between the container opening 31 and the cover 41, and also prevent the cover 41 from easily detaching from the container opening 31.

[0118] The cap 41 is a toothed cap, made of HDPE high-density polyethylene. The cap 41 adopts a top injection molding structure. The roundness deformation of the cap 41 after injection molding is less than 0.1mm, and the cap 41 is sturdy and not easily deformed when screwed on.

[0119] like Figure 10-11 As shown, in a more specific embodiment, the cell culture container 3 has multiple container ports 31, and each of the multiple container ports 31 is equipped with an adapter 4.

[0120] It should be noted that the cell culture container 3 has multiple container ports 31, and each of the multiple container ports 31 is equipped with a conversion connector 4, so that multiple conversion connectors 4 and multiple second connectors 5 can be connected accordingly. For example, when transferring liquid, the storage container 1 can transfer the cell suspension to the cell culture container 3 through multiple pipelines to speed up the transfer of liquid, or when ventilating, multiple venting components 2 can be connected to the cell culture container 3 to increase the amount of oxygen provided. Alternatively, some pipelines can be used to transfer liquid, while other pipelines are used for ventilating and oxygen supply.

[0121] In a more specific embodiment, one end of the plug-in connector 42 of the adapter 4 is inserted into the first hollow hole 411 and embedded in the container opening 31 of the cell culture container 3.

[0122] It should be noted that the end of the plug-in connector 42 inserted into the first hollow hole 411 is embedded in the container opening 31 of the cell culture container 3, which can effectively form a seal to prevent contamination and leakage.

[0123] All components of this application comply with USP Class VI standards and have no stimulating or affecting cell growth.

[0124] The working process of this utility model is as follows: the container opening 31 of the cell culture container 3 can be equipped with a conversion connector 4, the liquid storage container 1 and the venting component 2 can be connected to the second connector 5, and the insertion of the first connector 43 of the conversion connector 4 and the different second connectors 5 can quickly realize the conversion between the cell culture container 3 and the liquid storage container 1 and the venting component 2, reducing the time for liquid transfer and aeration culture conversion of the cell culture container 3, and effectively reducing the probability of contamination during the conversion process.

[0125] The plug-in connector 42 in the adapter 4 is inserted into the first hollow hole 411 of the cover 41, and the first connector 43 is inserted into the second hollow hole 421 of the plug-in connector 42, so as to achieve a sealed connection between the first connector 43 and the cover 41. The first connector 43 and the second connector 5 are inserted to form a sealed structure, which can ensure the airtightness of the entire adapter connection.

[0126] In summary, this utility model provides a liquid-sealed conversion component and a culture device, which can reduce the time for liquid transfer and aeration conversion of cell culture containers, effectively reduce the probability of contamination during the conversion process, and ensure the airtightness of the connection.

[0127] The above are merely preferred embodiments of this utility model. It should be noted that, for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of this utility model, and these improvements and substitutions should also be considered within the protection scope of this utility model.

Claims

1. A liquid-sealed conversion element, characterized in that, include: An adapter, the adapter comprising a cover, a plug-in connector, and a first connector; The cover has a first hollow hole, the plug-in connector is inserted into the first hollow hole and is sealed to the cover, the plug-in connector has a second hollow hole, the first connector is inserted into the second hollow hole and is sealed to the plug-in connector. The second connector is used to insert into the first connector and form a sealing structure.

2. The liquid-sealed conversion component according to claim 1, characterized in that, The first hollow hole includes a locking groove and a positioning groove that are connected and coaxial from bottom to top. The bottom of the locking groove penetrates the bottom wall of the cover, and the opening of the locking groove is connected to the bottom of the positioning groove. The diameter of the locking groove is larger than the diameter of the positioning groove. The plug-in connector includes a hollow tube and a first hollow boss connected and coaxially from bottom to top. The top of the hollow tube is connected to the bottom of the first hollow boss. The hollow hole of the hollow tube and the hollow hole of the first hollow boss are connected to form a second hollow hole. A ring of protrusions is provided around the outer periphery of the hollow tube near the top. The outer diameter of the protrusions is larger than the diameter of the positioning groove. The first hollow boss is embedded in the positioning groove, and the protrusions are located at the top of the locking groove.

3. The liquid-sealed conversion component according to claim 2, characterized in that, The first hollow hole also includes a skirt groove disposed above the positioning groove and coaxial with and connected to the positioning groove. The opening of the positioning groove is connected to the bottom of the skirt groove, and the opening of the skirt groove penetrates the top wall of the cover. The diameter of the positioning groove is smaller than the diameter of the skirt groove. The first hollow protrusion has a skirt around its outer periphery near the top; the outer diameter of the skirt is the same as the diameter of the skirt groove, the distance between the bottom of the skirt and the top of the protrusion is the same as the depth of the positioning groove, and the skirt is embedded in the skirt groove.

4. The liquid-sealed conversion component according to claim 2, characterized in that, The plug-in connector further includes a second hollow boss, the second hollow boss, the first hollow boss, and the hollow tube are coaxial, and the bottom of the second hollow boss is connected to the top of the first hollow boss; the hollow hole of the hollow tube, the hollow hole of the first hollow boss, and the hollow hole of the second hollow boss are connected to form the second hollow hole.

5. The liquid-sealed conversion component according to claim 4, characterized in that, The hollow hole of the second hollow boss includes a lower hollow hole and an upper hollow hole that are connected and coaxial from bottom to top. The bottom of the lower hollow hole is connected to the top of the hollow hole of the first hollow boss, the top of the lower hollow hole is connected to the bottom of the upper hollow hole, and the top of the upper hollow hole penetrates the top wall of the second hollow boss. The diameter of the upper hollow hole gradually increases from bottom to top.

6. The liquid-sealed conversion component according to claim 1, characterized in that, When the first connector is a male connector, the second connector is a female connector; or when the first connector is a female connector, the second connector is a male connector.

7. The liquid-sealed conversion component according to claim 6, characterized in that, The second connector has a insertion groove, which extends from one side of the outer peripheral wall of the second connector and through the other side of the outer peripheral wall of the second connector in the same horizontal direction; a insertion piece is inserted into the insertion groove, the insertion piece includes an insertion part and a limiting part, the limiting part and the insertion part are vertically connected, the limiting part is inserted into the insertion groove, and a limiting hole is formed on the limiting part, the semicircular diameter of the limiting hole near the insertion part is larger than the semicircular diameter of the limiting hole away from the insertion part; The first connector has a limiting groove around its outer periphery, and the inner diameter of the limiting groove is the same as the semicircular diameter of the limiting hole away from the insertion part.

8. The liquid-sealed conversion component according to claim 6, characterized in that, The first connector has a insertion groove, which extends from one side of the outer peripheral wall of the first connector and through the other side of the outer peripheral wall of the first connector in the same horizontal direction; a insertion piece is inserted into the insertion groove, the insertion piece includes an insertion part and a limiting part, the limiting part and the insertion part are vertically connected, the limiting part is inserted into the insertion groove, and a limiting hole is formed on the limiting part, the semicircular diameter of the limiting hole near the insertion part is larger than the semicircular diameter of the limiting hole away from the insertion part; The second connector has a limiting groove around its outer periphery, and the inner diameter of the limiting groove is the same as the semicircular diameter of the limiting hole away from the insertion part.

9. A closed liquid culture device, characterized in that, include: The liquid-sealed conversion element as described in any one of claims 1-8; A cell culture container, wherein the adapter is installed at the container opening of the cell culture container; A liquid storage container for storing cell suspension, the liquid storage container being connected to the second connector; A venting element for supplying oxygen to the cell culture container, the venting element being connected to the second connector.

10. The liquid-sealed culture device according to claim 9, characterized in that, It also includes a connecting plug for insertion with the first connector.

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