Nucleic acid detection reaction tube and reaction module

Abstract

The utility model relates to the technical field of nucleic acid detection kits, in particular to a nucleic acid detection reaction tube and a reaction module, and mainly relates to a nucleic acid detection reaction tube, the nucleic acid detection reaction tube comprises a tube body, the tube body is divided into a first section, a second section and a third section, the first section is in a tube shape with a circular section, the second section is arranged in a reducing mode from top to bottom, and the third section is arranged in the tube body. The third section is a cone with the oval section, a freeze-drying small ball is arranged in the tube body, the cross section of the freeze-drying small ball is larger than the minimum sectional area of the third section, and a gap is formed between the freeze-drying small ball and the bottom of the tube body. Compared with the prior art, the utility model has the following effects: in the second section, the second section is designed to be elliptical, and compared with a tubular body, the second section can contain less liquid under the same axial length, so that after the same reaction liquid is added, the generated tiny bubble layer is higher, and tiny bubbles on the upper layer can be better avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of nucleic acid detection kits, in particular to a nucleic acid detection reaction tube and a reaction module. BACKGROUND

[0002] In the field of detection kits, a certain specific substance is detected by mixing detection, for example, normal temperature constant nucleic acid rapid detection technology, which relies on recombination enzymes, single-stranded DNA binding proteins, DNA polymerases and specific primers in the system to complete the nucleic acid amplification reaction quickly under normal temperature constant (37-42 DEG C) conditions.

[0003] It mainly performs amplification in the reaction tube, and the lower end of the tube body is generally tubular. In the reaction process, a certain proportion of micro-bubbles is generated in the upper layer of the reaction liquid. In the actual reaction process, the part of the liquid that has been amplified in the reaction tube body also needs to be irradiated with a light spot to make it emit fluorescence. However, due to the existence of the micro-bubble layer, the effective area that can be irradiated is small, which affects the accuracy of detection. CONTENT OF THE UTILITY MODEL

[0004] In order to lift the micro-bubble layer and reduce its influence on light spot irradiation, the present application provides a nucleic acid detection reaction tube and a reaction module.

[0005] In the first aspect, the present application provides a nucleic acid detection reaction tube, which adopts the following technical scheme: a nucleic acid detection reaction tube, comprising a tube body, the tube body is divided into a first section, a second section and a third section, the first section is a tubular body with a circular cross section, the second section is provided with a diameter that decreases from top to bottom, and the third section is a conical body with an elliptical cross section, a freeze-dried ball is arranged in the tube body, the cross section of the freeze-dried ball is larger than the minimum cross-sectional area of the third section, and there is a gap between the freeze-dried ball and the bottom of the tube body.

[0006] By adopting the above technical scheme, the first section is mainly used for clamping the tube body, and it is also convenient for other reaction liquids to be dropped into. In the second section, the elliptical mode is adopted, compared with the tubular body, in the same axial length, the liquid capacity that can be accommodated is less, so that after the same reaction liquid is added, the micro-bubble layer generated will be higher, so that the upper micro-bubble layer can be better avoided, and the lowermost end of the freeze-dried ball is also in the reaction liquid. Compared with the elliptical shape of the second section, the cross section of the freeze-dried ball is circular, and there is a possibility that liquid flows through the two ends of the long axis of the second section cross section, so that the lower side of the freeze-dried ball can also smoothly contact the reaction liquid, thereby facilitating the reaction.

[0007] Preferably, the first section is connected with a gland, and the gland has an extension pipe embedded into the first section.

[0008] Preferably, the end of the extension pipe has a ring-shaped protrusion, and the inner wall of the first section has a limiting ridge for clamping the ring-shaped protrusion.

[0009] By adopting the technical scheme, the reagent in the pipe body can be sealed.

[0010] Preferably, the area of the gland is greater than the area of the end of the first section.

[0011] Preferably, the outer diameter of the extension pipe is smaller than the inner diameter of the first section.

[0012] Preferably, the end of the first section has a ring-shaped slope for abutting against the ring-shaped protrusion.

[0013] In another aspect, the application provides a nucleic acid detection reaction module, which adopts the following technical scheme: further comprising a reaction block for inserting the pipe body, the reaction block comprising a heat insulation part and a heating part, and the second section being located in the heating part.

[0014] Preferably, the heating part is provided with a light inlet channel and a light trapping channel, and the light inlet channel is perpendicular to the long axis of the cross section of the second section.

[0015] In summary, the application has at least one of the following beneficial technical effects:

[0016] 1. The first section is mainly used for clamping the pipe body, and also facilitates the dropping of other reaction liquid. In the second section, the second section is provided in an elliptical manner, compared with the tubular body, in the same axial length, the liquid volume that can be accommodated is less, so that after the same reaction liquid is added, the micro-bubble layer generated will be higher, so that the upper micro-bubble can be better avoided;

[0017] 2. At the same time, the lowermost position of the freeze-dried small ball also has reaction liquid. The cross section of the freeze-dried small ball is circular, compared with the elliptical shape of the second section, the long axis of the cross section of the second section has a certain possibility of liquid flowing through the two ends, so that the lower side of the freeze-dried small ball can also smoothly contact the reaction liquid, thereby facilitating the reaction. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 is a structural schematic diagram of embodiment 1 of the application;

[0019] Figure 2 is a partial sectional view of embodiment 1 of the application, for showing the position of the freeze-dried small ball in the pipe body;

[0020] Figure 3 is a top view of the pipe body when opened in embodiment 1 of the application;

[0021] Figure 4is a sectional view of the third segment in the third paragraph of Embodiment 1 of the present application, used to show the freeze-dried small balls in the gap between the inner walls of the third segment on the side;

[0022] Figure 5 is another partial sectional view of Embodiment 1 of the present application, used to show the connection relationship between the gland and the first segment;

[0023] Figure 6 is a sectional view of Embodiment 2 of the present application.

[0024] Legend: 100, first segment; 110, second segment; 111, third segment; 112, freeze-dried small balls; 113, gland; 114, extension tube; 115, annular protrusion; 116, limiting protrusion; 117, annular inclined surface; 120, reaction block; 121, heat insulation part; 122, heating part; 123, light inlet channel; 124, light trapping channel. DETAILED DESCRIPTION

[0025] The present application will be further described in detail below with reference to the accompanying drawings.

[0026] Embodiment 1: The present application discloses a nucleic acid detection reaction tube, referring to Figure 1 , Figure 2 , comprising a tube body, which is divided into a first segment 100, a second segment 110 and a third segment 111. The first segment 100 is a tube with a circular cross-section, the second segment 110 is arranged with a decreasing diameter from top to bottom, and the third segment 111 is an elliptical conical body.

[0027] Referring to Figure 2 , Figure 3 , a freeze-dried small ball 112 is arranged in the tube body. The cross-section of the freeze-dried small ball 112 is larger than the smallest cross-sectional area of the third segment 111, and the freeze-dried small ball 112 has a gap with the bottom of the tube body. The following is an example of arranging a single freeze-dried small ball 112 in the third segment 111. 25ul sample can be added to fully dissolve the small ball, and the total height of the liquid surface is 4mm. A 3mm light spot can be irradiated on the reaction tube. There is a small bubble layer of about 1mm on the upper layer, which does not affect the irradiation or detection.

[0028] Referring to Figure 2 , Figure 3 , Figure 4 , and the lower end of the third segment 111 is conical. When the freeze-dried small ball 112 is stuck, due to the horizontal cross-section being elliptical, the freeze-dried small ball 112 will be stuck at the short axis of the cross-section, and there is a liquid space between the long axis and the freeze-dried small ball 112. In this way, the reaction liquid will move downward into the gap, so that the lower side of the freeze-dried small ball 112 can also react smoothly.

[0029] Referring to Figure 1 , Figure 5The first section 100 is connected with a cover 113, and the cover 113 has an extension pipe 114 embedded into the first section 100. The end of the extension pipe 114 has a ring-shaped protrusion 115, and the inner wall of the first section 100 has a limiting protrusion 116 for clamping the ring-shaped protrusion 115. Meanwhile, the outer diameter of the extension pipe 114 is smaller than the inner diameter of the first section 100. The area of the cover 113 is larger than the area of the end of the first section 100. During the covering process, the extension pipe 114 will be able to be inserted into the first section 100 during the rotation, and the ring-shaped protrusion 115 will be clamped on the limiting protrusion 116 to achieve the fixing effect.

[0030] The inner edge of the end of the first section 100 has a ring-shaped slope 117 for abutting against the ring-shaped protrusion 115. Such ring-shaped slope 117 will play a guiding role when in contact with the ring-shaped protrusion 115, facilitating the covering during the rotation.

[0031] Embodiment 2: Based on the nucleic acid detection reaction tube described above, referring to Figure 2 The nucleic acid detection reaction module also includes a reaction block 120 for inserting the above-mentioned tube body, and the reaction block 120 includes a heat insulation part 121 and a heating part 122, and the second section 110 is located in the heating part 122. The heating part 122 is provided with a light inlet channel 123 and a light trapping channel 124, and the light inlet channel 123 is perpendicular to the long axis of the cross section of the second section 110.

[0032] Firstly, the third section 111 can be heated in a separate heating area without affecting the temperature of other parts, so that the third section 111 reaches the required reaction temperature, and meanwhile, the light inlet channel 123 and the light trapping channel 124 can increase the height of the micro-bubble layer and increase the irradiation area of the light inlet channel 123 directly to the third section 111 through the oval-shaped third section 111, facilitating the reaction.

[0033] The embodiments of the specific embodiment are the preferred embodiments of the present application, but do not limit the protection scope of the present application, so that: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A nucleic acid detection reaction tube, comprising a tube body, characterized in that: The tube is divided into a first section (100), a second section (110), and a third section (111). The first section (100) is a tube with a circular cross-section. The second section (110) is tapered from top to bottom. The third section (111) is a cone with an elliptical cross-section. Freeze-dried spheres (112) are provided inside the tube. The cross-section of the freeze-dried spheres (112) is larger than the minimum cross-sectional area of ​​the third section (111). There is a gap between the freeze-dried spheres (112) and the bottom of the tube.

2. The nucleic acid detection reaction tube according to claim 1, characterized in that: The first segment (100) is connected to a pressure cap (113), the pressure cap (113) having an extension tube (114) embedded in the first segment (100).

3. The nucleic acid detection reaction tube according to claim 2, characterized in that: The end of the extension tube (114) has an annular protrusion (115), and the inner wall of the first section (100) has a limiting protrusion (116) for engaging the annular protrusion (115).

4. A nucleic acid detection reaction tube according to claim 3, characterized in that: The area of ​​the cap (113) is larger than the area of ​​the end of the first segment (100).

5. A nucleic acid detection reaction tube according to claim 4, characterized in that: The outer diameter of the extension tube (114) is smaller than the inner diameter of the first segment (100).

6. A nucleic acid detection reaction tube according to claim 5, characterized in that: The inner edge of the end of the first segment (100) has an annular bevel (117) for abutting against the annular protrusion (115).

7. A nucleic acid detection reaction module, characterized in that: It also includes a reaction block (120) for inserting into the tube as described in any of claims 1-6, the reaction block (120) including a heat insulation part (121) and a heating part (122), the second segment (110) being located within the heating part (122).

8. The nucleic acid detection reaction module according to claim 7, characterized in that: The heating part (122) is provided with a light-entry channel (123) and a light-harvesting channel (124), and the light-entry channel (123) is perpendicular to the long axis of the second section (110).

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