Reagent uniform split-flow sample adding system

Abstract

The utility model relates to the technical field of reagent pipetting, in particular to a reagent uniform shunting and sample adding system which comprises a first reagent box, a first guide pipe, a switching valve, a second guide pipe, a first pump body, a third guide pipe, a first shunting part, a fourth guide pipe and a first injection needle which are sequentially connected, a flow inlet, a first flow channel, a second flow channel and flow outlets are formed in the first flow dividing part, the two ends of the first flow channel communicate with the two ends of the second flow channel correspondingly, the multiple flow outlets are linearly distributed in the second flow channel along the axis of the second flow channel, and each flow outlet is provided with an electromagnetic valve capable of controlling the flow outlet to be opened and closed. According to the sample adding system disclosed by the utility model, due to the arrangement of the shunting part and the electromagnetic valve, the injected reagent can be shunted, and when the reagent is injected, the sample adding efficiency can be obviously improved, and the sample adding workload can be reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to reagent pipetting technical field more particularly, relate to a reagent uniform shunt sample adding system. BACKGROUND

[0002] Immunohistochemistry is a method that uses the principle of specific binding between antigen and antibody in immunology, and determines the antigen in tissue cells by making the color developing agent of labeled antibody develop color through chemical reaction, and carries out positioning, qualitative and relative quantitative research. In the process of immunohistochemistry experiment, a plurality of different reagents such as hybridization solution, eluent, water, blocking solution, color developing solution and enzyme-labeled solution need to be pipetted, and each reagent is quantitatively delivered to the hole position on each reaction tank.

[0003] In the prior art, in order to ensure that the reagent injected into each hole position on the reaction tank is equal, the reagent is generally uniformly injected into each hole position on the reaction tank by using a pump to suck the reagent through a liquid injection needle. However, since the hole positions to be injected on the reaction tank are generally 30-50, the work load of reagent sampling will be doubled if each reagent is injected in this way, and the sampling efficiency is low and the work load is large. UTILITY MODEL CONTENT

[0004] The utility model aims at overcoming the deficiency of low sampling efficiency and large work load of the prior art reagent injection method for each hole position on the reaction tank, and provides a reagent uniform shunt sample adding system. The reagent sampling system in the utility model can shunt the injected reagent, achieve the purpose of simultaneously injecting a plurality of hole positions on the reaction tank, improve the sampling efficiency, and reduce the sampling work load.

[0005] To solve the above technical problems, the utility model adopts the technical scheme of:

[0006] The utility model provides a reagent uniform distribution flow sampling system, including first reagent box, switch valve, first pump body, first distribution part, first injection needle, first reaction tank, second conduit, third conduit and fourth conduit, be equipped with a plurality of containing cavities on the first reagent box, the reagent that each containing cavity on the first reagent box contains does not exist influence each other, the containing cavity of first reagent box with the feed port of switch valve one to one and pass through first conduit connection, the discharge port of switch valve passes through second conduit with the feed port of first pump body connection, be equipped with inflow port, first flow channel, second flow channel and a plurality of outflow ports on the first distribution part, the both ends of first flow channel communicate with the both ends of second flow channel respectively, a plurality of outflow ports are linearly arranged along the axis of second flow channel on second flow channel, be equipped with an electromagnetic valve on each outflow port, and the electromagnetic valve can control the opening and closing of the outflow port, the discharge port of first pump body passes through third conduit with the inflow port on the first distribution part connection, each outflow port passes through fourth conduit with a first injection needle, and the first injection needle can be inserted into the hole position on the first reaction tank, and the first injection needle is one to one with the hole position on the first reaction tank.

[0007] The reagent uniform distribution flow sampling system of the utility model carries out distribution flow sampling to the reagent that does not exist influence each other, first control switch valve switches different discharge port, so that one containing cavity on the first reagent box is connected with the first distribution part through the first conduit and the second conduit, and the first pump body starts to work, drives the reagent in the containing cavity to pass through the first conduit, switch valve, second conduit and third conduit in turn, and enters into the first distribution part along the inflow port on the first distribution part. The reagent that enters into the first distribution part continues to advance under the drive of the first pump body, and the reagent will first fill the first flow channel, then enters into the both ends of the second flow channel from the both ends of the first flow channel, and the reagent that enters into the both ends of the second flow channel converges to the midpoint of the second flow channel. Among a plurality of outflow ports on the second flow channel, first open the electromagnetic valve of the outflow port at the midpoint position of the second flow channel, close the electromagnetic valve on the outflow port when the first injection needle connected with the outflow port flows out the reagent, then insert all first injection needles into the hole position on the first reaction tank, ensure that there is a first injection needle in each hole position, then open the electromagnetic valve on a plurality of outflow ports simultaneously, so that the reagent at each outflow port flows into the first injection needle through the fourth conduit connected with it, and flows into the hole position on the first reaction tank through the first injection needle, complete the equal and simultaneous sampling of a plurality of hole positions on the first reaction tank. When different reagents need to be added, the feed port on the switch valve can be changed to complete the switching of the reagent to be added.

[0008] The utility model discloses a reagent uniform shunting sampling system, through the setting of two flow channels in the first shunting part, the flow of several outflow ports can be completely same, when the reagent that does not exist influence each other is filled, can obviously improve the sampling efficiency, reduces the workload of sampling.

[0009] Further, the several outflow ports are equidistantly arranged on the second flow channel, and the inflow port is located at the midpoint of the first flow channel. The inflow port is located at the midpoint of the first flow channel, so that the reagent can fill the first flow channel through the inflow port, and the reagent at both ends of the first flow channel can enter the second flow channel at the same time, further ensuring that the reagent at both ends of the second flow channel converges at the midpoint of the second flow channel, and ensuring that the second flow channel has been filled with reagent when the outflow port at the midpoint of the second flow channel has reagent flowing out. The several outflow ports are equidistantly arranged on the second flow channel, which is more convenient to ensure that the flow of each outflow port is the same, and is also more convenient to connect the fourth conduit with the outflow port.

[0010] Further, the inner diameter of the third conduit is greater than the inner diameter of the first conduit, the inner diameter of the first conduit is equal to the inner diameter of the second conduit, and the inner diameter of the second conduit is greater than the inner diameter of the fourth conduit. Since the reagent in the first conduit and the second conduit flows into the third conduit, the reagent in the third conduit needs to be divided and enter the several fourth conduits, and experiments show that when the inner diameter of the third conduit is greater than the inner diameter of the first conduit, the inner diameter of the first conduit is equal to the inner diameter of the second conduit, and the inner diameter of the second conduit is greater than the inner diameter of the fourth conduit, the flow rate of the reagent flowing out of the first injection needle can be ensured to be the same, so that the reagent entering the several holes of the first reaction tank is equal.

[0011] Further, the first pump body is a plunger pump; and the switching valve is a rotary switching valve. The plunger pump has the advantages of high efficiency and large transmission power. The use of the plunger pump can make the reagent flow in the conduit larger, and the flow of the plunger pump is easy to adjust. The flow of the plunger pump divided by the number of the first injection needles can obtain the flow in each hole of the first reaction tank. The first pump body can also be a peristaltic pump or other driving elements capable of driving the reagent to advance in the pipeline. The rotary switching valve is easy to operate. The first conduit, the second conduit, the third conduit and the fourth conduit are all transparent PTFE tubes. The PTFE tube (polytetrafluoroethylene tube) has the advantages of resistance to all strong acids, strong bases and strong oxidizing agents, and no reaction with various organic solvents. In addition, the PTFE tube also has the advantages of small friction, heat resistance, water resistance and aging resistance. The transparent tube can observe the advancing position of the reagent during sampling, which is more convenient for the operator to find the advancing path of the reagent in time.

[0012] The utility model also provides another reagent uniform shunt sampling system, including second reagent box, second reaction tank, a plurality of second pump body, a plurality of second shunt parts and a plurality of second injection needle, a plurality of containing cavities on the second reagent box, there is mutual influence between the reagent filled in each containing cavity on the second reagent box, the second shunt part is equipped with one inflow and a plurality of outflow, each containing cavity on the second reagent box is connected with the feed inlet of one second pump body through the fifth conduit, the discharge outlet of each second pump body is connected with the inflow of one second shunt part through the sixth conduit, each sixth conduit is equipped with one pipe valve, the outflow of each second shunt part is connected with one second injection needle through the seventh conduit, and the liquid outlet of each second injection needle can be located in one hole position on the second reaction tank. When each reagent is filled, the reagent in the containing cavity on the second reagent box sequentially passes through the fifth conduit, the second pump body, the sixth conduit, the second shunt part, the seventh conduit and the second injection needle and enters the hole position on the second reaction tank. Each reagent has a pipeline composed of a separate second pump body, a second shunt part and a second injection needle, so as to avoid the reaction of different reagents before entering the second reaction tank and affect the effect of the reagent.

[0013] Further, the reagent uniform distribution and injection system further comprises the above-mentioned reagent uniform distribution and injection system, the first distribution part and the second distribution part are the same in structure, the second pump body and the first pump body are the same in structure, and the first reaction tank and the second reaction tank are the same reaction tank. The first reaction tank and the second reaction tank are the same reaction tank, that is, after the first injection needle is used to inject the reagent into the hole position on the reaction tank, the second injection needle can be used to inject the reagent into the same hole position on the same reaction tank again, that is, the reagent in the second reagent box can be injected into the hole position of the first reaction tank through the second injection needle, and the reagent in the first reagent box can be injected into the hole position of the second reaction tank through the first injection needle.

[0014] Further, the reagent uniform distribution and injection system further comprises the above-mentioned reagent uniform distribution and injection system, the first distribution part and the second distribution part are the same in structure, the second pump body and the first pump body are the same in structure, and the first reaction tank and the second reaction tank are the same reaction tank. The first reaction tank and the second reaction tank are the same reaction tank, that is, after the first injection needle is used to inject the reagent into the hole position on the reaction tank, the second injection needle can be used to inject the reagent into the same hole position on the same reaction tank again, that is, the reagent in the second reagent box can be injected into the hole position of the first reaction tank through the second injection needle, and the reagent in the first reagent box can be injected into the hole position of the second reaction tank through the first injection needle.

[0015] Further, the hole positions on the reaction tank are arranged in an array on the reaction tank, and the reagent uniform distribution and injection system further comprises a fixing frame, the fixing frame is provided with a plurality of first mounting holes and a plurality of second mounting holes, the first mounting holes and the second mounting holes are arranged in an array on the fixing frame, the first injection needle is arranged in the first mounting hole, and the second injection needle is arranged in the second mounting hole. The fixing frame is arranged, so that when the first injection needle and the second injection needle are used to inject the reagent into the hole positions on the reaction tank, the reaction tank only needs to be moved, and the injection needles do not need to be moved into the hole positions on the reaction tank one by one, thereby further reducing the workload during reagent injection.

[0016] Further, the reagent uniform distribution and injection system further comprises the above-mentioned reagent uniform distribution and injection system, the first distribution part and the second distribution part are the same in structure, the second pump body and the first pump body are the same in structure, and the first reaction tank and the second reaction tank are the same reaction tank. The first reaction tank and the second reaction tank are the same reaction tank, that is, after the first injection needle is used to inject the reagent into the hole position on the reaction tank, the second injection needle can be used to inject the reagent into the same hole position on the same reaction tank again, that is, the reagent in the second reagent box can be injected into the hole position of the first reaction tank through the second injection needle, and the reagent in the first reagent box can be injected into the hole position of the second reaction tank through the first injection needle.

[0017] Further, the inner diameter of the sixth conduit is greater than the inner diameter of the fifth conduit, and the inner diameter of the fifth conduit is greater than the inner diameter of the seventh conduit. It is experimentally measured that when the inner diameter of the sixth conduit is greater than the inner diameter of the fifth conduit, and the inner diameter of the fifth conduit is greater than the inner diameter of the seventh conduit, the same reagent flow rate from the second injection needle can be ensured, so that the reagents entering the holes of the reaction tank are equal. The fifth conduit, the sixth conduit and the seventh conduit for transporting light-proof reagents are black PTFE pipes; and the fifth conduit, the sixth conduit and the seventh conduit for transporting non-light-proof reagents are transparent PTFE pipes. The light-proof reagents are transported by black pipes to avoid light; and the non-light-proof reagents are transported by transparent pipes, so that the advancing position of the reagents can be seen in real time.

[0018] Compared with the prior art, the reagent uniform distribution sampling system has the following beneficial effects:

[0019] The reagent uniform distribution sampling system has the following beneficial effects: the flow rates at the plurality of outflow openings are completely the same due to the arrangement of the two flow channels in the first distribution part, the sampling efficiency is obviously improved, and the sampling workload is reduced when the reagents that do not affect each other are filled.

[0020] The electromagnetic valve is arranged at each outflow opening of the first distribution part of the reagent uniform distribution sampling system, the opening and closing of each outflow opening can be independently controlled, the number of the working first injection needles is further independently controlled, the number of the working first injection needles can be flexibly adjusted according to the number of the holes on the specific first reaction tank when the reagents are filled into the first reaction tanks of different models, and the reagent filling into the first reaction tanks of more different models is applicable.

[0021] The reagent uniform distribution sampling system further has the following beneficial effects: each reagent has a separate pipeline composed of a second pump body, a second distribution part and a second injection needle, so that the different reagents do not react before entering the reaction tank, and the effect of the reagents is affected. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 It is a structural schematic view of a reagent uniform distribution sampling system;

[0023] Figure 2 It is an internal structure schematic view of a first distribution part of a reagent uniform distribution sampling system;

[0024] Figure 3 It is another structural schematic view of a reagent uniform distribution sampling system;

[0025] Figure 4 It is a structural schematic view of an injection needle and a fixing frame of a reagent uniform distribution sampling system;

[0026] Figure 5 It is a structural schematic view of a shunt part and a shunt die seat of a reagent uniform shunt sampling system.

[0027] In the drawings: 1, first reagent box; 2, rotary switching valve; 3, first plunger pump; 4, first shunt part; 5, first injection needle; 6, first reaction tank; 7, first conduit; 8, second conduit; 9, third conduit; 10, fourth conduit; 11, fixed frame; 401, inlet; 402, outlet; 403, first flow channel; 404, second flow channel; 12, second reagent box; 13, second plunger pump; 14, second shunt part; 15, second injection needle; 16, shunt die seat; 17, electromagnetic valve; 18, mounting bracket; 19, pipe valve. DETAILED DESCRIPTION

[0028] The utility model will be further explained in connection with specific implementation. Among them, the drawings are only for example description, and the representation is only schematic diagram, and cannot be understood as the limitation of the patent; in order to better illustrate the embodiment of the utility model, some components of the drawings will be omitted, enlarged or reduced, and the size of actual product is not represented; for those skilled in the art, it is understandable that some well-known structures and their description in the drawings can be omitted.

[0029] The same or similar reference numerals in the drawings of the embodiments of the utility model correspond to the same or similar components; in the description of the utility model, it is understood that if the orientation or position relationship indicated by the terms "upper", "lower", "left", "right" etc. is based on the orientation or position relationship shown in the drawings, only for the convenience of describing the utility model and simplifying the description, and not indicating or implying that the indicated device or element must have a particular orientation, a particular orientation and operation, therefore the position relationship description in the drawings is only for example description, and cannot be understood as the limitation of the patent, for the ordinary skilled in the art, the specific meaning of the above terms can be understood according to the specific situation.

[0030] Embodiment one

[0031] The embodiment is a first embodiment of a reagent uniform shunt sampling system, as shown in Figure 1 And Figure 2As shown, it comprises a first reagent box 1, a switching valve 2, a first pump body 3, a first flow splitter 4, a first injection needle 5, a first reaction tank 6, a first conduit 7, a second conduit 8, a third conduit 9 and a fourth conduit 10. The first reagent box 1 is provided with a plurality of accommodation cavities for respectively containing reagents that do not affect each other. The accommodation cavities are in one-to-one correspondence with the feed ports of the switching valve 2 and are connected to the feed ports through the first conduit 7. The discharge port of the switching valve 2 is connected to the feed port of the first pump body 3 through the second conduit 8. The first flow splitter 4 is provided with one inflow port 401. The first flow splitter 4 is further provided with a first flow channel 403, a second flow channel 404 and a plurality of outflow ports 402. The two ends of the first flow channel 403 are respectively in communication with the two ends of the second flow channel 404. The plurality of outflow ports 402 are linearly and equidistantly arranged on the second flow channel 404 along the axis of the second flow channel 404. The inflow port 401 is located at the midpoint of the first flow channel 403. Each outflow port 402 is provided with an electromagnetic valve 17 capable of controlling the opening and closing of the outflow port 402. The discharge port of the first pump body 3 is connected to the inflow port 401 of the first flow splitter 4 through the third conduit 9. Each outflow port 402 is connected to a first injection needle 5 through the fourth conduit 10. The first injection needle 5 can be inserted into the hole position on the first reaction tank 6, and the first injection needle 5 is in one-to-one correspondence with the hole position on the first reaction tank 6.

[0032] Specifically, the switching valve 2 is a rotary switching valve. The first pump body 3 is a plunger pump. The inner diameter of the third conduit 9 is 2 mm. The inner diameters of the first conduit 7 and the second conduit 8 are 1.5 mm. The inner diameter of the fourth conduit 10 is 1 mm. The first conduit 7, the second conduit 8, the third conduit 9 and the fourth conduit 10 are all transparent PTFE tubes.

[0033] The working principle or working process of the embodiment is as follows:

[0034] The sample adding system is used in the following way: first, different reagents which do not affect each other are respectively filled in the cavities of the first reagent box 1, then the switching valve 2 is controlled to switch different discharge ports, so that one cavity of the first reagent box 1 is connected with the first shunt part 4 through the first conduit 7 and the second conduit 8, and the first pump body 3 is operated to drive the reagents in different cavities to sequentially pass through the first conduit 7, the switching valve 2, the second conduit 8 and the third conduit 9, and then enter the first shunt part 4 through the inflow port 401 of the first shunt part 4. The reagents entering the first shunt part 4 continue to move under the driving of the plunger pump, first move along the middle point of the first flow channel 403 to the two sides of the first flow channel 403, then the reagents enter the two ends of the second flow channel 404 from the two ends of the first flow channel 403, and then gather along the two ends of the second flow channel 404 to the middle point of the second flow channel 404. Among the several outflow ports 402 of the second flow channel 404, first, the electromagnetic valve 17 at the outflow port 402 at the middle point of the second flow channel 404 is opened, when the reagent flows out of the first injection needle 5 connected with the outflow port 402, the electromagnetic valve 17 at the outflow port 402 is closed, then all the first injection needles 5 are inserted into the hole positions of the first reaction tank 6, so that each hole position has one first injection needle 5, then the electromagnetic valves 17 at the several outflow ports 402 are simultaneously opened, so that the reagents at each outflow port 402 flow into the first injection needle 5 through the fourth conduit 10 connected with the outflow port 402, and then flow into the hole positions of the first reaction tank 6 through the first injection needle 5, thus completing the equal and simultaneous sample adding of the several hole positions of the first reaction tank 6. When different reagents need to be added, the discharge port of the rotating switching valve 2 is changed to complete the switching.

[0035] The beneficial effects of the embodiment are as follows:

[0036] The sample adding system is used in the following way: first, different reagents which do not affect each other are respectively filled in the cavities of the first reagent box 1, then the switching valve 2 is controlled to switch different discharge ports, so that one cavity of the first reagent box 1 is connected with the first shunt part 4 through the first conduit 7 and the second conduit 8, and the first pump body 3 is operated to drive the reagents in different cavities to sequentially pass through the first conduit 7, the switching valve 2, the second conduit 8 and the third conduit 9, and then enter the first shunt part 4 through the inflow port 401 of the first shunt part 4. The reagents entering the first shunt part 4 continue to move under the driving of the plunger pump, first move along the middle point of the first flow channel 403 to the two sides of the first flow channel 403, then the reagents enter the two ends of the second flow channel 404 from the two ends of the first flow channel 403, and then gather along the two ends of the second flow channel 404 to the middle point of the second flow channel 404. Among the several outflow ports 402 of the second flow channel 404, first, the electromagnetic valve 17 at the outflow port 402 at the middle point of the second flow channel 404 is opened, when the reagent flows out of the first injection needle 5 connected with the outflow port 402, the electromagnetic valve 17 at the outflow port 402 is closed, then all the first injection needles 5 are inserted into the hole positions of the first reaction tank 6, so that each hole position has one first injection needle 5, then the electromagnetic valves 17 at the several outflow ports 402 are simultaneously opened, so that the reagents at each outflow port 402 flow into the first injection needle 5 through the fourth conduit 10 connected with the outflow port 402, and then flow into the hole positions of the first reaction tank 6 through the first injection needle 5, thus completing the equal and simultaneous sample adding of the several hole positions of the first reaction tank 6. When different reagents need to be added, the discharge port of the rotating switching valve 2 is changed to complete the switching.

[0037]

[0038] ​The reagents are driven forward by the plunger pump, the flow of the plunger pump is adjusted conveniently, and the flow of each hole in the reaction tank is obtained by dividing the flow by the number of the first injection needle. The rotary switching valve switches the liquid path conveniently. When the third conduit is larger than the inner diameter of the first conduit 7, the inner diameter of the first conduit 7 is equal to the inner diameter of the second conduit 8 and is larger than the inner diameter of the fourth conduit 10, the uniform and equal effect of the reagents is best. The PTFE tube (polytetrafluoroethylene tube) has the advantages of resisting all strong acids, strong bases and strong oxidants, not reacting with various organic solvents, small friction, heat resistance, water resistance and aging resistance. The transparent tube can observe the forward position of the reagents during sampling, which is more convenient for the operator to find the forward path of the reagents in time.

[0039] Embodiment two

[0040] The embodiment is a second embodiment of a reagent uniform distribution sampling system, as shown in Figures 3-5 The sampling system of the embodiment comprises a second reagent box 12, a second reaction tank, a plurality of second pump bodies 13, a plurality of second distribution parts 14 and a plurality of second injection needles 15, a plurality of containing cavities on the second reagent box 12 are used to contain different reagents which affect each other, the second distribution part 14 is provided with an inflow port and a plurality of outflow ports, the containing cavities on each second reagent box 12 are connected with the inflow port of a second pump body 13 through a fifth conduit, the outflow port of each second pump body 13 is connected with the inflow port 401 of a second distribution part 14 through a sixth conduit, a pipe valve 19 is arranged on each sixth conduit, the pipe valve 19 is an electromagnetic valve, the outflow port 402 of each second distribution part 14 is connected with a second injection needle 15 through a seventh conduit, and the outflow end of each second injection needle 15 can be located in a hole of the second reaction tank.

[0041] The beneficial effects of the embodiment are as follows: each reagent in the sampling system of the embodiment has a pipeline composed of a separate second pump body 13, a second distribution part 14 and a second injection needle 15, so that the reaction of different reagents before entering the second reaction tank is avoided, and the effect of the reagents is affected.

[0042] Embodiment three

[0043] The embodiment is based on embodiment two, as shown in Figures 3-5 The structure of the sampling system is further limited.

[0044] Specifically, it further comprises a reagent uniform distribution sampling system as described in embodiment one, the structures of the first distribution part 4 and the second distribution part 14 are the same, the structures of the first pump body 3 and the second pump body 13 are the same, and the first reaction tank 6 and the second reaction tank are the same reaction tank.

[0045] Specifically, the shunt die holder 16 is included, the first shunt part 4 and the second shunt part 14 are located on the shunt die holder 16, the inlet port 401 and the outlet port 402 of the first shunt part 4 and the inlet port 401 and the outlet port 402 of the second shunt part 14 are located on the same side of the shunt die holder 16, the electromagnetic valve 17 on the first shunt part 4 and the electromagnetic valve 17 of the second shunt part 14 are located on the same side of the shunt die holder 16, and the electromagnetic valve 17 of the first shunt part 4 and the inlet port 401 of the first shunt part 4 are located on the opposite sides of the shunt die holder 16.

[0046] Specifically, as shown in Figure 2 the holes on the reaction tank are arranged in an array on the reaction tank, and the fixed frame 11 is further included, the fixed frame 11 is provided with a plurality of first mounting holes and a plurality of second mounting holes, the first mounting holes and the second mounting holes are arranged in an array on the fixed frame, the first injection needle 5 is arranged in the first mounting hole, and the second injection needle 15 is arranged in the second mounting hole.

[0047] Specifically, the mounting rack 18 is further included, and the fixed frame 11 and the shunt die holder 16 are mounted on the mounting rack 18.

[0048] Specifically, the inner diameter of the sixth conduit is 2mm, the inner diameter of the fifth conduit is 1.5mm, and the inner diameter of the seventh conduit is 1mm. The fifth conduit, the sixth conduit and the seventh conduit for transporting light-avoiding reagents are black PTFE tubes; and the fifth conduit, the sixth conduit and the seventh conduit for transporting non-light-avoiding reagents are transparent PTFE tubes.

[0049] The beneficial effects of the embodiment are as follows:

[0050] The sample adding system of the embodiment can also add reagents that do not react with each other in batches.

[0051] The shunt die holder 16 integrates the first shunt part 4 and the second shunt part 14 together, which saves more space on the experiment table. The inlet port 401 and the outlet port 402 are connected with the conduits, so they are arranged on the same side of the shunt die holder 16, and the electromagnetic valve 17 is arranged on the back of the shunt die holder 16, so that the space utilization rate of the shunt die holder 16 is higher.

[0052] The fixed frame 11 is arranged, so that when the injection needle is used to add reagents to the holes on the reaction tank, the reaction tank only needs to be moved, and the injection needle does not need to be moved to the holes on the reaction tank one by one, which further reduces the workload when reagents are added.

[0053] The installation frame 18 is arranged to facilitate positioning and installation of the fixed frame 11 and the shunt mold base 16. When the inner diameter of the sixth conduit is greater than the inner diameter of the fifth conduit, and the inner diameter of the fifth conduit is greater than the inner diameter of the seventh conduit, the uniform and equal effect of the reagent is best. The light-avoiding reagent is transported by a black pipe to avoid the reagent from being exposed to light; the non-light-avoiding reagent is transported by a transparent pipe, and the advancing position of the reagent can be seen in real time.

[0054] In the specific contents of the above specific embodiments, each technical feature can be combined arbitrarily without contradiction. In order to make the description simple, all possible combinations of the above technical features are not described, but as long as the combination of the technical features does not exist, it should be considered as the scope of the present disclosure.

[0055] Obviously, the above embodiments of the present application are only examples for clearly illustrating the present application, and are not intended to limit the embodiments of the present application. For those skilled in the art, other different forms of changes or modifications can be made on the basis of the above description. Here, it is not necessary and impossible to enumerate all the embodiments. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the claims of the present application.

Claims

1. A reagent uniform distribution and sample injection system, characterized in that, The utility model provides a kind of automatic injection system, including first kit (1), switching valve (2), first pump body (3), first shunt (4), first injection needle (5), first reaction tank (6), first conduit (7), second conduit (8), third conduit (9) and fourth conduit (10), the first kit (1) is equipped with several containing cavities, there is no mutual influence between the reagent filled in each containing cavity on the first kit (1), the containing cavity of first kit (1) is corresponding with the feed inlet of switching valve (2) and is connected by first conduit (7), the discharge outlet of switching valve (2) is connected with the feed inlet of first pump body (3) by second conduit (8), first shunt (4) is equipped with inflow port (401), first flow channel (403), second flow channel (404) and several outflow ports (402), both ends of first flow channel (403) are communicated with both ends of second flow channel (404) respectively, several outflow ports (402) are linearly arranged on second flow channel (404) along the axis of second flow channel (404), an electromagnetic valve (17) for controlling the opening and closing of each outflow port (402) is arranged on each outflow port (402), the discharge outlet of first pump body (3) is connected with inflow port (401) by third conduit (9), each outflow port (402) is connected with a first injection needle (5) by fourth conduit (10), and the first injection needle (5) can be inserted into the hole position on the first reaction tank (6), and the first injection needle (5) is corresponding with the hole position on the first reaction tank (6).

2. The reagent uniform distribution and injection system according to claim 1, characterized in that, Several outflow ports (402) are equidistantly arranged on second flow channel (404), and inflow port (401) is located at the midpoint of first flow channel (403).

3. The reagent uniform distribution and injection system according to claim 1, characterized in that, The inner diameter of third conduit (9) is greater than the inner diameter of first conduit (7), the inner diameter of first conduit (7) is equal to the inner diameter of second conduit (8), and the inner diameter of second conduit (8) is greater than the inner diameter of fourth conduit (10).

4. The reagent uniform distribution and injection system according to claim 1, characterized in that, First pump body (3) is a plunger pump, and switching valve (2) is a rotary switching valve.

5. A reagent uniform distribution and injection system, characterized in that, The second kit (12) comprises a plurality of containing cavities, the reagents contained in each containing cavity of the second kit (12) interact with each other, the second flow distributor (14) is provided with an inlet and a plurality of outlets, each containing cavity of the second kit (12) is connected to the inlet of one of the second pumps (13) through a fifth conduit, the outlet of each second pump (13) is connected to the inlet of one of the second flow distributors (14) through a sixth conduit, each sixth conduit is provided with a valve (19), and the outlet of each second flow distributor (14) is connected to one of the second injection needles (15) through a seventh conduit.

6. The reagent uniform distribution and injection system according to claim 5, characterized in that, The reagent uniform distribution and injection system also comprises the first flow distributor (4) and the second flow distributor (14) which are identical in structure, the second pump (13) which is identical in structure to the first pump (3), and the first reaction tank (6) and the second reaction tank which are one and the same reaction tank.

7. The reagent uniform distribution and injection system according to claim 6, characterized in that, The reagent uniform distribution and injection system also comprises a flow distributor base (16), the first flow distributor (4) and the second flow distributor (14) are located on the flow distributor base (16), the inlet (401) and the outlet (402) of the first flow distributor (4) and the inlet (401) and the outlet (402) of the second flow distributor (14) are located on the same side of the flow distributor base (16), the electromagnetic valve (17) of the first flow distributor (4) and the electromagnetic valve (17) of the second flow distributor (14) are located on the same side of the flow distributor base (16), and the electromagnetic valve (17) of the first flow distributor (4) and the inlet (401) of the first flow distributor (4) are located on opposite sides of the flow distributor base (16), respectively.

8. The reagent uniform distribution and injection system according to claim 7, characterized in that, The holes on the reaction tank are arranged in an array on the reaction tank, and the reagent uniform distribution and injection system also comprises a fixing frame (11) provided with a plurality of first mounting holes and a plurality of second mounting holes, the first mounting holes and the second mounting holes are arranged in an array on the fixing frame (11), and the first injection needle (5) is arranged in the first mounting hole, and the second injection needle (15) is arranged in the second mounting hole.

9. The reagent uniform distribution and injection system of claim 8, wherein, The reagent uniform distribution and injection system also comprises a mounting rack (18), and the fixing frame (11) and the flow distributor base (16) are mounted on the mounting rack (18).

10. The reagent uniform distribution and injection system of claim 5, wherein, The inner diameter of the sixth conduit is greater than the inner diameter of the fifth conduit, and the inner diameter of the fifth conduit is greater than the inner diameter of the seventh conduit.

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