Cryopreservation device using liquid betaine for cryopreservation of cells

Abstract

The utility model relates to the technical field of cryopreservation devices for cryopreservation of cells, in particular to a cryopreservation device for cryopreservation of cells by using liquid betaine, which comprises a liquid betaine storage tank and a freezing tank, and an infusion pump is fixedly connected to one side, far away from the liquid betaine storage tank, of a tank body output pipe. A first injection pipe is fixedly connected to the output end of the infusion pump, a second input port and a third input port are formed in the detachable cover body, a base body is fixedly connected to the bottom end in the freezing tank, a notch is formed in one side of the top of the base body, a freezing cell test tube is arranged on one side in the notch, and a freezing cell is arranged in the freezing cell test tube. A swinging structure fixedly connected with the driving end below the electric telescopic rod is arranged on the other side of the interior of the notch; the frozen cell test tube is driven by the swing structure to move back and forth, so that a cell suspension and liquid betaine in the frozen cell test tube can be mixed, and the trouble of manual shaking is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to the freezing device technical field of frozen cell, specifically to a kind of liquid betaine is used for the freezing device of frozen cell. BACKGROUND

[0002] Betaine, as a natural osmotic pressure regulator, has the characteristics of non-toxic and good biocompatibility, and has been studied for the protection of cryopreservation of cells. During the freezing process, betaine can protect cells from freezing injury through various mechanisms, such as inhibiting ice crystal formation and reducing osmotic damage.

[0003] However, at present, when liquid betaine is used for cryopreservation of cells, it is usually necessary to manually add liquid betaine to the cells and shake to achieve cryopreservation of the cells. This method is not efficient as a whole.

[0004] Therefore, it is necessary to design a freezing device for cryopreservation of cells using liquid betaine to solve the above problems. UTILITY MODEL CONTENT

[0005] The utility model aims to provide a kind of freezing device for cryopreservation of cells using liquid betaine to solve the problems raised in the above background.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0007] A kind of freezing device for cryopreservation of cells using liquid betaine, comprising liquid betaine storage tank and freezing tank, the top of the liquid betaine storage tank is provided with detachable cover, the detachable cover is provided with first input and tank body output, the upper side of the tank body output is connected with tank body output pipe by screw thread, the side of the tank body output pipe away from the liquid betaine storage tank is fixedly connected with infusion pump, the output end of the infusion pump is fixedly connected with first injection pipe, the top of the freezing tank is fixedly installed with detachable cover, and the detachable cover is provided with second input and third input, the second input is fixedly connected with second injection pipe inside, the bottom of the third input is fixedly connected with auxiliary injection pipe, the inside bottom end of the freezing tank is fixedly connected with seat, the inside of the seat is fixedly installed with refrigeration device, the upper side of the refrigeration device is fixedly provided with exhaust fan, the top of the seat is provided with notch on one side, the inside of the notch is provided with frozen cell test tube on one side, and the bottom of the frozen cell test tube is connected with electric telescopic rod, the inside of the notch is provided with swing structure fixedly connected with the driving end below the electric telescopic rod on the other side.

[0008] As the preferred scheme of the utility model, the swing structure includes the reciprocating air cylinder which is located at the side of the gap far away from the frozen cell test tube, the front end of the reciprocating air cylinder is fixedly connected to the outside of the driving end of the electric telescopic rod through the welding process.

[0009] As the preferred scheme of the utility model, the bottom middle position of the frozen cell test tube is provided with an internal thread groove, the top telescopic end of the electric telescopic rod is provided with an external thread groove, and the bottom of the frozen cell test tube and the top of the telescopic end of the electric telescopic rod are connected through threads.

[0010] As the preferred scheme of the utility model, the distance sensor is embedded and installed on the inner wall of the shorter side of the side of the gap far away from the reciprocating air cylinder, the distance sensor is used for detecting the distance between the distance sensor and the electric telescopic rod, and the distance sensor and the control end of the reciprocating air cylinder are electrically connected.

[0011] As the preferred scheme of the utility model, the top of the frozen cell test tube is provided with two through holes, and the second injection pipe and the auxiliary injection pipe penetrate into the inside of the two through holes.

[0012] As the preferred scheme of the utility model, the top cold air outlet end of the refrigeration device is also provided with a cold guide plate in communication, the top of the cold guide plate is in contact with the inside of the gap, so that the cold air is transmitted to the frozen cell test tube.

[0013] As the preferred scheme of the utility model, the detachable cover body located on the liquid betaine storage tank and the freezing tank and the peripheral side of the liquid betaine storage tank and the freezing tank are fixedly locked through the lock catch.

[0014] Compared with the prior art, the utility model has the beneficial effects that:

[0015] 1. In the utility model, a freezing device for freezing cells by using liquid betaine is set, the freezing device adopts an infusion pump to inject the liquid betaine in the liquid betaine storage tank into the frozen cell test tube in the freezing tank, and cells are previously placed in the frozen cell test tube, so that the liquid betaine and the cell suspension are mixed, and the subsequent freezing operation is facilitated.

[0016] 2. In the utility model, a freezing device for freezing cells by using liquid betaine is set, the swing structure drives the frozen cell test tube to move back and forth, so that the cell suspension in the frozen cell test tube and the liquid betaine are mixed, and the trouble of manual shaking is avoided. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the whole three-dimensional structure schematic diagram of the utility model;

[0018] Figure 2 It is the inside structure schematic view of the freezing tank of the utility model;

[0019] Figure 3 It is the overhead structure schematic view of the utility model seat body;

[0020] Figure 4 It is the inside main structure schematic view of the utility model seat body.

[0021] In the drawing: 1, liquid betaine storage tank; 2, freezing tank; 21, detachable cover; 3, first input port; 4, tank body output port; 5, tank body output pipe; 6, infusion pump; 7, first injection pipe; 8, second input port; 9, third input port; 10, second injection pipe; 11, auxiliary injection pipe; 12, seat body; 13, refrigeration device; 14, exhaust fan; 15, gap; 16, frozen cell test tube; 161, electric telescopic rod; 17, swing structure; 171, reciprocating air cylinder; 18, distance sensor; 19, cold guide plate; 20, lock catch. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0023] In order to facilitate the understanding of the utility model, the utility model will be described more fully below with reference to the relevant drawings. Several embodiments of the utility model are given. However, the utility model can be realized in many different forms, and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the utility model more thorough and comprehensive.

[0024] It should be noted that when an element is referred to as "fixed to" another element, it can be directly on the other element or there can be a middle element. When an element is referred to as "connected" to another element, it can be directly connected to the other element or there can be a middle element. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description herein is for describing particular embodiments only and is not intended to be limiting of the application. The use herein of the terms "including", "comprising", "having" and the like are specifically intended to be construed in an inclusive sense and not to the exclusion of any other items.

[0026] Embodiments, please refer to Figures 1-4 The application provides a technical scheme:

[0027] A kind of liquid betaine is used for the freezing device of frozen cell, including liquid betaine storage tank 1 and freezing tank 2, the top of liquid betaine storage tank 1 is provided with detachable cover 21, detachable cover 21 is provided with first input 3 and tank body output 4, the top of tank body output 4 is connected with tank body output pipe 5 by screw thread, tank body output pipe 5 is fixedly connected with infusion pump 6 on the side away from liquid betaine storage tank 1, the output end of infusion pump 6 is fixedly connected with first injection pipe 7, first injection pipe 7 is connected using detachable pipeline of multiple sections, the top of freezing tank 2 is fixedly installed with detachable cover 21, and second input 8 and third input 9 are arranged in the detachable cover 21, second injection pipe 10 is fixedly connected in the inside of second input 8, auxiliary injection pipe 11 is fixedly connected in the bottom of third input 9, the inside bottom end of freezing tank 2 is fixedly connected with seat body 12, the top side of seat body 12 is provided with aperture 15, freezing cell test tube 16 is arranged in the inside side of aperture 15, infusion pump 6 is used to inject liquid betaine in the inside of liquid betaine storage tank 1 into freezing cell test tube 16 in the inside of freezing tank 2, and cell is placed in freezing cell test tube 16 in advance, so that liquid betaine and cell suspension are mixed.

[0028] Specifically, the other side of the inside of aperture 15 is provided with swing structure 17, swing structure 17 includes reciprocating cylinder 171 on the side away from freezing cell test tube 16 in the inside of aperture 15, the front end telescopic end of reciprocating cylinder 171 is fixedly connected to the outside of the lower drive end of electric telescopic rod 161 by welding process;

[0029] In this embodiment, the telescopic end of reciprocating cylinder 171 is driven to move back and forth to make freezing cell test tube 16 reciprocate, so that cell suspension in the inside of freezing cell test tube 16 and liquid betaine are mixed.

[0030] The notch 15 is embedded with a distance sensor 18 on the short side inner wall away from the reciprocating cylinder 171. The distance sensor 18 is used to detect the distance between the distance sensor 18 and the electric telescopic rod 161, and the distance sensor 18 and the control end of the reciprocating cylinder 171 are electrically connected. Through the detection of the distance sensor 18, the reciprocating cylinder 171 can drive the frozen cell test tube 16 to move to the original position, so as to facilitate the next freezing operation.

[0031] The bottom of the frozen cell test tube 16 is connected with the electric telescopic rod 161, the top of the frozen cell test tube 16 is provided with two through holes, and the second injection pipe 10 and the auxiliary injection pipe 11 penetrate into the two through holes. The electric telescopic rod 161 can drive the frozen cell test tube 16 to move up and down, so that the second injection pipe 10 and the auxiliary injection pipe 11 are placed in the frozen cell test tube and separated from the frozen cell test tube.

[0032] The bottom of the frozen cell test tube 16 is connected with the electric telescopic rod 161, the top of the frozen cell test tube 16 is provided with two through holes, and the second injection pipe 10 and the auxiliary injection pipe 11 penetrate into the two through holes. The electric telescopic rod 161 can drive the frozen cell test tube 16 to move up and down, so that the second injection pipe 10 and the auxiliary injection pipe 11 are placed in the frozen cell test tube and separated from the frozen cell test tube.

[0033] Specifically, the inside of the seat body 12 is fixedly installed with a refrigeration device 13, the upper side of the refrigeration device 13 is fixedly provided with an exhaust fan 14, and the top of the refrigeration device 13 is further communicated with a cold air guide plate 19. The top of the cold air guide plate 19 is in contact with the inside of the notch 15, so as to transfer the cold air to the frozen cell test tube 16.

[0034] In this embodiment, the refrigeration device 13 can work and produce cold air, which is finally conducted to the frozen cell test tube 16 under the action of the exhaust fan 14 to perform the freezing operation.

[0035] Specifically, the detachable cover 21 on the liquid betaine storage tank 1 and the freezing tank 2 and the periphery of the liquid betaine storage tank 1 and the freezing tank 2 are locked and fixed by the lock buckle 20.

[0036] In this embodiment, the detachable cover 21 on the liquid betaine storage tank 1 and the freezing tank 2 can be opened by opening the lock buckle 20.

[0037] The utility model discloses a working procedure: when using the liquid betaine for the cryopreservation device of cryopreservation cell, first according to cell type and experimental demand, select suitable betaine concentration. Different cell types are different to the sensitivity of betaine, therefore need through pre-experiment to determine optimum concentration, and a proper amount of liquid betaine is dissolved in proper solvent (such as physiological saline, PBS etc.), and preparation becomes cryopreservation fluid. Ensure that the solution is sterile and pH is appropriate, when cryopreservation, adopt infusion pump 6 and inject the liquid betaine in the liquid betaine storage jar 1 inside to the cryogenic tank 2 inside cryogenic cell test tube 16 of liquid betaine, and cryogenic cell test tube 16 has placed cell in advance, to make liquid betaine and cell suspension mix, in the process of mixing, through the back and forth movement of the telescopic end of reciprocating cylinder 171 to drive cryogenic cell test tube 16 to reciprocate, and then make the cell suspension and liquid betaine in cryogenic cell test tube 16 mix. After mixing, through the work of refrigeration device 13 and produce cold air, and the cold air is finally conducted to cryogenic cell test tube 16 under the action of exhaust fan 14, to carry out the cryopreservation of cell suspension and liquid betaine, after the complete cryopreservation of cell, can be transferred to liquid nitrogen tank and kept for a long time. In liquid nitrogen tank, cell can keep very low temperature, thereby prolongs the preservation time.

[0038] Although the embodiments of the utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made thereto without departing from the principles and spirit of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A freezing device for freezing cells using liquid betaine, comprising a liquid betaine storage tank (1) and a freezing tank (2), characterized in that: The detachable cover (21) is arranged on the top of the liquid betaine storage tank (1), and a first input port (3) and a tank body output port (4) are arranged on the detachable cover (21); a tank body output pipe (5) is threadedly connected above the tank body output port (4); a transfusion pump (6) is fixedly connected to the side of the tank body output pipe (5) away from the liquid betaine storage tank (1); a first injection pipe (7) is fixedly connected to the output end of the transfusion pump (6); the detachable cover (21) is fixedly installed on the top of the freezing tank (2), and a second input port (8) and a third input port (9) are arranged on the detachable cover (21); a second injection pipe (10) is fixedly connected in the second input port (8); an auxiliary injection pipe (11) is fixedly connected to the bottom of the third input port (9); a seat body (12) is fixedly connected to the inside bottom end of the freezing tank (2); a refrigeration device (13) is fixedly installed in the inside of the seat body (12); an exhaust fan (14) is fixedly arranged above the refrigeration device (13); an opening (15) is formed on one side of the top of the seat body (12); a freezing cell test tube (16) is arranged on one side in the inside of the opening (15), and an electric telescopic rod (161) is connected to the bottom of the freezing cell test tube (16); and a swing structure (17) is arranged on the other side in the inside of the opening (15) and fixedly connected to the driving end below the electric telescopic rod (161).

2. The cryopreservation device of claim 1, wherein the liquid betaine is used for cryopreservation of cells. The swing structure (17) comprises a reciprocating air cylinder (171) arranged on the side of the opening (15) away from the freezing cell test tube (16).

3. A cryogenic device for cryopreservation of cells using liquid betaine according to claim 2, characterized in that: An internal thread groove is formed at the middle position of the bottom of the freezing cell test tube (16), an external thread groove is formed at the top telescopic end of the electric telescopic rod (161), and the bottom of the freezing cell test tube (16) is connected to the top telescopic end of the electric telescopic rod (161) through threads.

4. The cryogenic device for cryopreserving cells using liquid betaine according to claim 2, wherein: A distance sensor (18) is embedded and installed on the inner wall of the shorter side of the opening (15) away from the reciprocating air cylinder (171), the distance sensor (18) is used for detecting the distance between the distance sensor (18) and the electric telescopic rod (161), and the distance sensor (18) is electrically connected to the control end of the reciprocating air cylinder (171).

5. The device for cryopreservation of cells using liquid betaine according to claim 1, wherein: Two through holes are formed on the top of the freezing cell test tube (16), and the second injection pipe (10) and the auxiliary injection pipe (11) penetrate into the inside of the two through holes.

6. The cryogenic device of claim 1, wherein the liquid betaine is used for cryopreservation of cells. A cold guide plate (19) is further connected and arranged at the cold air outlet end of the top of the refrigeration device (13), and the top of the cold guide plate (19) is in contact with the inside of the opening (15) to transfer cold air to the freezing cell test tube (16).

7. The use of a liquid betaine for the cryopreservation of cells according to claim 1, characterized in that: The detachable cover (21) on the liquid betaine storage tank (1) and the freezing tank (2) and the periphery of the liquid betaine storage tank (1) and the freezing tank (2) are locked and fixed by the lock catch (20).

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