Quantitative tangential flow filtering system without stirring pipeline

Abstract

The utility model provides a tangential flow filtering system capable of quantifying without a stirring pipeline, which comprises a product tank, a bottom outlet of the product tank is connected with a liquid inlet main pipeline through a pipeline, a circulating pump is arranged on the liquid inlet main pipeline, and an outlet end of the liquid inlet main pipeline is connected with a liquid inlet end of a tangential flow filtering membrane bag. A backflow end of the tangential flow filtering membrane bag is connected with a liquid outlet main pipeline, the liquid outlet main pipeline is connected with a liquid inlet end of a tubular container, a liquid outlet end of the tubular container is respectively connected with the liquid inlet main pipeline and a collection end through pipelines, and a permeation end of the tangential flow filtering membrane bag is connected with a liquid discharge pipeline; during use, the tubular container replaces a product tank to serve as a part of functions of a circulation tank, and meanwhile reverse mixing of the circulation tank is brought about; the utility model has the advantages that the flux of the membrane is improved, and the concentration and re-filtration can be completed more quickly.

Description

TECHNICAL FIELD

[0001] The utility model relates to the filtration system in the biological medicine production, chemical industry and food field, especially in a kind of tangential flow filtration system without stirring pipeline ration. BACKGROUND

[0002] The most common filtration downstream of biopharmaceutical is tangential flow filtration, i.e., TFF, which is mainly used for clarification, product concentration and buffer filtration. Figure 1 The existing tangential flow filtration system mainly includes a tank container, a stirrer and a weighing device at the bottom of the tank container, the bottom of the tank container is connected to the inlet of a tangential flow module through a pump, the backflow end of the tangential flow module is connected to the inlet of the tank container through a pipeline, and the permeation end is connected to a collection container.

[0003] The tangential flow filtration system in the prior art has the following defects: a stirring device and a weighing device need to be added at the bottom of the tank container; as a circulating tank, the tank container will cause severe reverse mixing when the liquid from the backflow end enters the tank, which is not conducive to the flux of the tangential flow filtration module; the overall structure of the system is not compact, which is not conducive to cleaning and sterilization; and the yield of the product is low. UTILITY MODEL CONTENT

[0004] To overcome the above-mentioned deficiencies of the prior art, the utility model provides a tangential flow filtration system without stirring pipeline ration, which can make the tank container not serve as a circulating tank, and does not need to add a stirring device and a weighing device at the bottom of the tank, so that the structure is compact; reverse mixing does not occur, the fluid is pushed horizontally, and the flux is improved.

[0005] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0006] To solve the above-mentioned technical problems, the first aspect of the disclosed content of the utility model provides a tangential flow filtration system without stirring pipeline ration, which includes a product tank, the bottom outlet of the product tank is connected to a liquid inlet main pipeline through a pipeline, a circulating pump is arranged on the liquid inlet main pipeline, the outlet end of the liquid inlet main pipeline is connected to the liquid inlet end of a tangential flow filtration membrane package, the backflow end of the tangential flow filtration membrane package is connected to a liquid outlet main pipeline, the liquid outlet main pipeline is connected to the liquid inlet end of a tubular container, the liquid outlet end of the tubular container is connected to the liquid inlet main pipeline and a collection end through a pipeline respectively, and the permeation end of the tangential flow filtration membrane package is connected to a liquid discharge pipeline.

[0007] In some embodiments, the tubular container is a coiled or folded pipe or pipeline.

[0008] In some embodiments, the diameter of the tubular container is not more than 2 times the diameter of the liquid inlet main pipe and / or the liquid outlet main pipe.

[0009] In some embodiments, a mixer is arranged on the liquid inlet main pipe, and the connecting end between the liquid outlet end of the tubular container and the liquid inlet main pipe is located between the circulating pump and the mixer.

[0010] In some embodiments, an adjusting pump is arranged on the liquid outlet pipe.

[0011] In some embodiments, a bypass pipe is communicated with the liquid inlet end of the tubular container, and a valve is arranged on the pipe.

[0012] In some embodiments, the liquid inlet port of the liquid inlet main pipe is communicated with the displacement tank through a pipe.

[0013] In some embodiments, valves are respectively arranged on the liquid outlet port of the product tank and the connecting pipe.

[0014] In some embodiments, valves are respectively arranged on the liquid outlet port of the product tank and the connecting pipe.

[0015] The beneficial effects of the present application are as follows:

[0016] 1. The tubular container is connected between the liquid inlet end and the return end of the tangential flow filtration membrane package, which replaces part of the function of the product tank as a circulating tank, and brings about reverse mixing of the circulating tank, thus improving the flux of the membrane, which enables the system to complete concentration and refiltration faster.

[0017] 2. The use of the tubular container and the tank circulation greatly reduces the consumption and time of cleaning and sterilization.

[0018] 3. The product tank only serves the function of filling product liquid, and does not need to have bottom stirring and weighing functions, so that the overall structure is compact.

[0019] 4. The volume of the tubular container is quantified according to the volume of the product in the product tank, so that different pipes can be configured to improve the flexibility and convenience of the process.

[0020] 5. The product concentration at the liquid inlet end of the tubular container is greater than the concentration at the liquid outlet end of the tubular container, and the solution realizes flat pushing in the tubular container, which is more conducive to the filtration of the product. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is a structural schematic view of the prior art tangential flow filtration system.

[0022] Figure 2 It is a structural schematic view of the embodiment 1 of the present application.

[0023] Figure 3 Figure 2 is a structural schematic diagram of the embodiment 2 of the present application.

[0024] Figure 4 Figure 3 is a structural schematic diagram of the embodiment 3 of the present application.

[0025] Figure 5 Figure 4 is a structural schematic diagram of the embodiment 4 of the present application. DETAILED DESCRIPTION

[0026] The present application will be further described below with reference to the drawings.

[0027] The technical content of the present application is described below through specific embodiments, and other advantages and effects of the present application can be easily understood by those skilled in the art from the content disclosed in the present specification. The present application can also be implemented or applied through other different specific embodiments. Those skilled in the art can make various modifications and changes without departing from the spirit of the present application.

[0028] Before the specific embodiments of the present disclosure are described in detail, some terms used in the present disclosure are first explained.

[0029] Unless otherwise defined in the following, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Reference herein to technical terms used herein is intended to refer to the technical terms as commonly understood by those skilled in the art, including variations or substitutions of the technology that are obvious to those skilled in the art or equivalent technology. Although the following terms are believed to be well understood by those skilled in the art, the following definitions are set forth to better explain the present application. When a trade name appears herein, it is intended to refer to its corresponding product. All patents, published patent applications, and publications recited herein are incorporated by reference herein.

[0030] The terms "connected", "connected to", "in communication with", or "coupled" and like terms as used herein are not limited to direct connections, but also include indirect connections.

[0031] The "product" as described herein is a biomolecule, including proteins, nucleic acids, lipids, carbohydrates, small nucleotides, amino acids, and derivatives thereof.

[0032] The term "on-line monitoring" or "real-time monitoring" as used herein refers to detecting certain parameters or properties of the buffer, reaction fluid, fluid flowing out of the flow reactor, such as pH value, pressure, flow rate, conductivity, etc. in real time during the use of the chromatography system. Unlike off-line detection or analysis, on-line monitoring or real-time monitoring can provide real-time feedback of the detection results.

[0033] The positional relationship words "on", "under", "left", "right", "front", "back" and the like referred to in this document are determined according to the layout direction of the drawings, and are only used to represent relative positional relationship. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0034] The application scenario of the patent is laboratory scale, pilot test, pilot production and large-scale production, and it is suitable for the biopharmaceutical industry with high value, high sensitivity and strict aseptic requirements.

[0035] Referring to Figure 2 As shown in the figure, the embodiment 1 is a tangential flow filtration system without stirring pipeline quantitative, including product tank 1, the bottom of product tank is provided with valve one 2, the outlet end of product tank 1 is connected with liquid inlet main pipeline 3 through connecting pipeline, the connecting pipeline is provided with valve two 4, the liquid inlet main pipeline 3 is provided with circulating pump 5 and mixer 6, the outlet end of liquid inlet main pipeline 3 is connected with the liquid inlet end of tangential flow filtration membrane package 7, the backflow end of tangential flow filtration membrane package 7 is connected with the liquid inlet end of tubular container 10 through liquid outlet main pipeline 8, the liquid outlet main pipeline 8 is provided with regulating pump 9, the liquid outlet end of tubular container 10 is connected with liquid inlet main pipeline 3 through connecting pipeline, the connecting point is located between circulating pump 5 and mixer 6, and is connected with collection end 12, the connecting pipeline is provided with valve three 13 and valve four 11 respectively. The liquid inlet end of tubular container 10 is also connected with bypass pipeline 14, the bypass pipeline 14 is provided with valve five 15, the tubular container 10 adopts coiled or folded pipe or pipeline, the coiled or folded direction of which is horizontal direction, the diameter of tubular container is not more than 2 times of liquid outlet main pipeline, and the volume of tubular container is quantitatively determined according to the volume of product in product tank. The permeation end of tangential flow filtration membrane package 7 is connected with liquid outlet pipeline 16.

[0036] When the product tank 1 is used, open the valve one 2, valve two 4, circulating pump 5, regulating pump 9 and valve three 13, the product liquid enters into the tangential flow filtration membrane pack 7 through the liquid inlet main pipe 3, part of the small molecule solution permeates the filtration membrane and enters into the liquid outlet pipe 16, the remaining material enters into the tank container 10 through the liquid outlet main pipe 8 from the tangential flow filtration membrane pack 7 backflow port, and then flows into the bamboo liquid inlet main pipe 3; then the product liquid in the product tank 1 enters into the tangential flow filtration membrane pack 7 again under the mixing effect of the circulating pump 5 output solution and the material in the tubular container in the mixer 6, so that a loop is formed between the tangential flow filtration membrane pack 7 and the tubular container, the circulating pump 2 provides the tangential flow speed, according to the set flow rate requirement, the circulating pump 2 speed is adjusted to achieve the set flow rate, and a steady state is reached, the regulating pump 9 is used to increase the suction to actively control the tangential flow speed, which can provide the suction on the tangential flow filtration membrane pack 7 membrane, and the tangential flow is actively controlled sensitively. When the product liquid reaches the concentration process end point, stop the circulating pump 2 and the regulating pump 9, close the tank bottom valve one 2, valve two 4, valve three 13, and the product is concentrated in a quantitative way through the pipeline, and the product is recovered, at this time, open the valve five 15 and the valve four 11, and pressurize to make the product flow from the tubular container 10 into the collection end 12 for collection.

[0037] Referring to Figure 3 As shown in FIG. 2, the tangential flow filtration system without stirring pipeline quantification of embodiment 2 comprises a product tank 21, the bottom of the product tank 21 is provided with a valve one 22, the outlet end of the product tank 21 is connected with a liquid inlet main pipe 23 through a connecting pipe, the connecting pipe is provided with a valve two 24, the liquid inlet main pipe 23 is provided with a circulating pump 25 and a mixer 26, the liquid outlet end of the liquid inlet main pipe 23 is connected with the liquid inlet end of a tangential flow filtration membrane pack 27, the backflow end of the tangential flow filtration membrane pack 27 is connected with the liquid inlet end of a tubular container 210 through a liquid outlet main pipe 28, the liquid outlet main pipe 28 is provided with a regulating pump 29, the liquid outlet end of the tubular container 210 is connected with the liquid inlet main pipe 23 through a connecting pipe, the connecting point is located between the circulating pump 25 and the mixer 26, and the connecting pipe is connected with a collection end 212, the connecting pipe is provided with a valve three 213 and a valve four 211. The liquid inlet end of the tubular container 210 is also connected with a branch pipe 214, the branch pipe 214 is provided with a valve five 215, the tubular container 210 adopts a coiled or folded pipe or pipeline, the coiled or folded direction of the tubular container is a vertical direction, the diameter of the tubular container is not greater than 2 times the diameter of the liquid outlet main pipe, and the volume of the tubular container is quantified according to the volume of the product in the product tank. The permeation end of the tangential flow filtration membrane pack 27 is connected with a liquid outlet pipe 216.

[0038] Referring to Figure 4As shown in the figure, the embodiment 3 is a kind of tangential flow filtration system without stirring pipe quantitative, including product tank 31, product tank bottom is provided with valve one 32, product tank 31 export end is connected through connecting pipe into liquid main pipe 23, connecting pipe is provided with valve two 34, into liquid main pipe 33 is provided with circulating pump 35 and mixer 36, into liquid main pipe 33 outlet end connects the inlet end of tangential flow filtration membrane package 37, tangential flow filtration membrane package 37 backflow end is connected through outlet main pipe 38 the inlet end of tubular container 310, outlet main pipe 38 is provided with regulating pump 39, the outlet end of tubular container 310 is connected into liquid main pipe 33 through connecting pipe, connecting point is located between circulating pump 35 and mixer 36, and is connected collection end 312, connecting pipe is provided with valve three 313 and valve four 31 respectively.The inlet end of tubular container 310 is also connected to the side branch pipe 314, the side branch pipe 314 is provided with valve five 315, the tubular container 310 adopts coiled or folded tube or pipe, the coiled or folded direction of the tubular container has a certain angle with the horizontal direction, the diameter of the tubular container is not more than 2 times of the outlet main pipe, and the volume of the tubular container is quantified according to the volume of the product in the product tank.The permeation end of tangential flow filtration membrane package 37 is connected to the drain pipe 316.

[0039] Referring to Figure 5 As shown in the figure, the embodiment 3 is a kind of tangential flow filtration system without stirring pipe quantitative, including product tank 31, product tank bottom is provided with valve one 32, product tank 31 export end is connected through connecting pipe into liquid main pipe 23, connecting pipe is provided with valve two 34, into liquid main pipe 33 is provided with circulating pump 35 and mixer 36, into liquid main pipe 33 outlet end connects the inlet end of tangential flow filtration membrane package 37, tangential flow filtration membrane package 37 backflow end is connected through outlet main pipe 38 the inlet end of tubular container 310, outlet main pipe 38 is provided with regulating pump 39, the outlet end of tubular container 310 is connected into liquid main pipe 33 through connecting pipe, connecting point is located between circulating pump 35 and mixer 36, and is connected collection end 312, connecting pipe is provided with valve three 313 and valve four 31 respectively.The inlet end of tubular container 310 is also connected to the side branch pipe 314, the side branch pipe 314 is provided with valve five 315, the tubular container 310 adopts coiled or folded tube or pipe, the coiled or folded direction of the tubular container has a certain angle with the horizontal direction, the diameter of the tubular container is not more than 2 times of the outlet main pipe, and the volume of the tubular container is quantified according to the volume of the product in the product tank.The permeation end of tangential flow filtration membrane package 37 is connected to the drain pipe 316.

[0040] When the product tank 41 is used, the tank bottom valve 1 42, valve 2 44, circulating pump 45, regulating pump 49 and valve 3 413 are opened, and the product liquid enters the tangential flow filtration membrane package 47 through the liquid inlet main pipe 43, part of the small molecule solution permeates the filtration membrane and enters the liquid outlet pipe 416, and the remaining material enters the tank container 410 through the liquid outlet main pipe 48 through the tangential flow filtration membrane package 47 backflow port, and then flows into the bamboo liquid inlet main pipe 43; then the product liquid in the product tank 41 is output by the circulating pump 45, and the material in the tubular container is mixed in the mixer 46 to enter the tangential flow filtration membrane package 47, so that a loop is formed between the tangential flow filtration membrane package 47 and the tubular container, the circulating pump 42 provides the tangential flow speed, the circulating pump 2 speed is adjusted according to the set flow rate requirement to achieve the set flow rate and reach the steady state, and the regulating pump 49 is used to increase the suction to actively control the tangential flow speed, which can provide the suction on the tangential flow filtration membrane package 47 membrane and actively control the tangential flow. When fresh buffer solution is needed, the displacement tank 417 bottom valve 6 418 and valve 7 419 are opened at this time, and the buffer solution enters the liquid inlet main pipe 43.

[0041] The pressure sensor 420 and the flow meter 421 are arranged on the liquid inlet main pipe 43 to monitor the pressure and flow rate in real time, the suction of the regulating pump 49 is adjusted through the actual transmembrane pressure difference, so as to achieve the transmembrane pressure difference.

[0042] When the product liquid reaches the concentration process end point, the circulating pump 42 and the regulating pump 49 are stopped, the tank bottom valve 1 42, valve 2 44, valve 3 43, valve 6 418 and valve 7 419 are closed, the product is concentrated by the pipeline quantitative method, the product is recovered, and the valve 5 45 and the valve 4 41 are opened at this time.

Claims

1. A tangential flow filtration system for dosing without agitation of a pipe, characterized in that, The product tank is connected with the inlet main pipeline through a pipeline, the inlet main pipeline is provided with a circulating pump, the outlet end of the inlet main pipeline is connected with the inlet end of the tangential flow filter membrane package, the return end of the tangential flow filter membrane package is connected with the outlet main pipeline, the outlet main pipeline is connected with the inlet end of the tubular container, the outlet end of the tubular container is connected with the inlet main pipeline and the collection end through a pipeline respectively, and the permeation end of the tangential flow filter membrane package is connected with the liquid discharge pipeline.

2. A tangential flow filtration system for dosing a pipe without agitation according to claim 1, characterized in that The tubular container is a coiled or folded tube or pipeline.

3. A tangential flow filtration system for dosing a pipe without agitation according to claim 2, characterized in that The diameter of the tubular container is not more than 2 times of the outlet main pipeline and / or the inlet main pipeline.

4. A tangential flow filtration system for dosing a pipeline without agitation according to claim 1, characterized in that The inlet main pipeline is provided with a mixer, and the connecting end between the outlet end of the tubular container and the inlet main pipeline is located between the circulating pump and the mixer.

5. A tangential flow filtration system for dosing a pipeline without agitation according to claim 1, wherein, The liquid outlet pipeline is provided with an adjusting pump.

6. A tangential flow filtration system for dosing a pipeline without agitation according to claim 1, characterized in that The inlet end of the tubular container is communicated with a branch pipeline, and the pipeline is provided with a valve.

7. A tangential flow filtration system for dosing a pipeline without agitation according to claim 1, characterized in that The inlet port of the inlet main pipeline is communicated with a displacement tank through a pipeline.

8. A tangential flow filtration system for dosing a pipe without agitation according to claim 7, characterized in that Valves are respectively arranged on the liquid outlet of the displacement tank and the connecting pipeline.

9. A tangential flow filtration system for dosing a pipeline without agitation according to claim 1, wherein, Valves are respectively arranged on the liquid outlet of the product tank and the connecting pipeline.

Images