Full-automatic device for repairing and dyeing tissue sections

Abstract

The utility model provides a full-automatic device for repairing and dyeing tissue slices. A cooling and liquid discharging system is arranged on a frame body of the full-automatic device, a heating block is arranged on the cooling and liquid discharging system, a glass slide frame is arranged above the heating block, and a glass slide is arranged above the glass slide frame; the frame body is also provided with a driving assembly, the driving assembly is connected with a pressing structure and a dragging plate, the pressing structure can press the cover plate, the cover plate and the glass slide are in one-to-one correspondence to form a reaction cavity, and the head of the cover plate is connected with the dragging plate. According to the utility model, the problems of poor dyeing consistency effect, insufficient tissue repair effect and dry sheet are solved.

Description

Technical Field

[0001] This utility model relates to the field of automated machinery technology, specifically to a fully automated device for tissue section repair and staining. Background Technology

[0002] The fully automated immunohistochemistry device is used to complete the entire IHC process from slide baking to counterstaining, which greatly reduces the cost of manual operation and effectively avoids human error. It allows for precise control of the amount of working solution, temperature and time required for each step of the experiment, thereby obtaining uniform and high-quality staining results.

[0003] Fully automated immunohistochemistry devices employ a technique called "liquid coverslip." This process eliminates the need for traditional glass coverslips, instead using a plastic slide that forms a cavity that covers the specimen, creating an effect similar to a physical coverslip. In this way, the reaction area is confined directly beneath the added reagent, ensuring concentrated and uniform contact. During operation, the slide ensures the sample remains moist and stable during staining and incubation, preventing external contamination or interference. Furthermore, the slide effectively reduces reagent evaporation, improving experimental accuracy and reproducibility.

[0004] Chinese patent CN212134283 discloses an automated staining drive device for use with the slide cover plate disclosed in patent 201711340983.3, proposing a structure capable of dragging the cover plate. It achieves the translational locking and lifting of the cover plate through the cooperation of a third auxiliary plate and a follower. However, in the existing device, during the experimental heating process, some moisture inevitably disappears from the reaction chamber between the cover plate and the slide, leading to varying degrees of dryness in the upper reaction area and insufficient sample activity. This results in inconsistent staining and inadequate tissue repair effects in the experimental results. Utility Model Content

[0005] This invention provides a fully automated device for tissue section repair and staining, which aims to solve the problems of poor staining consistency, insufficient tissue repair effect, and dry sections in existing automated staining drive devices.

[0006] Technical means:

[0007] This invention proposes a fully automated device for tissue section repair and staining. The device includes a cooling and drainage system on the frame, a heating block on the cooling and drainage system, a slide holder above the heating block, and a glass slide placed on the slide holder. The frame also includes a drive assembly, which is connected to a pressing structure and a drag plate. The pressing structure can press down a cover plate, and the cover plate and the glass slide correspond one-to-one to form a reaction chamber. The head of the cover plate is connected to the drag plate.

[0008] Furthermore, the drive assembly includes a drive plate, a small cam, a support frame, a guide shaft, a column guide shaft, a guide rail, a slide block, a large cam, a support plate, a first roller, a second roller, a third roller, a fourth roller, and a fifth roller. The guide shaft and the column guide shaft are mounted on the frame. The support frame is slidably connected to the guide shaft. A guide rail is fixed above the support frame. A slide block is slidably connected to the guide rail. The slide block is an L-shaped plate. The short side of the L-shaped plate passes through the support plate and is fixedly connected to the drive plate. A drag plate is fixedly connected to the long side of the L-shaped plate. The support frame is equipped with a first roller, which cooperates with the small cam. The support plate is slidably connected to the column guide shaft. A pressing structure is fixedly connected to the top of the support plate. The support plate is equipped with a second roller and a third roller. The second roller cooperates with the large cam, and the third roller cooperates with the drive plate. The large cam is also equipped with a fourth roller and a fifth roller, both of which cooperate with the drive plate.

[0009] Furthermore, the drive board is provided with a series of curved grooves, long grooves and short grooves.

[0010] Furthermore, the pressing structure includes an upper cover and multiple sets of pressing block assemblies disposed below it.

[0011] Furthermore, the pressing block assembly consists of a pressing block mounting base, at least one pressing block, a pin, and a torsion spring. The pressing block mounting base has a number of pressing block slots matching the number of pressing blocks. One end of the pressing block is fixed in the pressing block slot by the pin and the torsion spring, and the other end of the pressing block is used to press down on the cover plate. A limiting block is provided in the pressing block slot. One side of the pressing block touches the limiting block, and the other side touches the torsion spring. The limiting block limits the tilt angle of the pressing block. This tilt angle satisfies the requirement that when the waste liquid returns, the surface of the pressing block facing the cover plate is parallel to the inclined surface at the tail of the cover plate.

[0012] Furthermore, the cooling and drainage system includes a duct base plate, a heating block fixing plate, a negative pressure fan, a waste liquid collection bottle, and a negative pressure fan interface. The duct base plate is mounted on the frame, and the heating block fixing plate is mounted above the duct base plate. A heat dissipation outlet, a waste liquid discharge outlet, a drain outlet, and an air inlet are provided below the duct base plate. The heat dissipation outlet is used to connect to the negative pressure fan, and the waste liquid discharge outlet is connected to the negative pressure fan interface through the waste liquid collection bottle.

[0013] Furthermore, the waste liquid collection bottle includes a bottle body and a guide tube. The guide tube is located inside the bottle body and extends from the top of the bottle body to the bottom of the bottle body. An airflow port is opened at the top of the bottle body. The guide tube is connected to the waste liquid discharge port, and the airflow port is connected to the negative pressure fan interface.

[0014] Furthermore, the heating block fixing plate is a rectangular groove with a ventilation drain rack. The upper surface of the ventilation drain rack has multiple heating grooves and drain holes, with each heating groove and drain hole corresponding to the previous one. Heating blocks are placed in the heating grooves, and the number of heating grooves corresponds to the number of heating blocks. The drain holes are connected to the waste liquid outlet on the bottom plate of the ventilation duct. The inner wall of the ventilation drain rack is provided with air ducts that correspond to each heating groove. One end of the air duct corresponds to the air inlet on the bottom plate of the air duct, and the other end of the air duct is located below the heating block.

[0015] Furthermore, the heating block includes a heating plate, with fins fixed below the heating plate. A through hole is provided between the fins and the heating plate, and a heating rod is placed in the through hole. A clip is also provided below one end of the heating plate, and the clip engages with the drain hole of the heating block fixing plate.

[0016] Furthermore, the drag plate is provided with multiple drag tabs with openings at the top.

[0017] Beneficial effects:

[0018] This invention provides a fully automated device for tissue section repair and staining. Through a clever design of the drive plate structure, a bent arc groove is incorporated compared to existing drive plates. This curved arc groove restricts the forward extension of the drag plate, and the cover plate and slide only move relative to each other on the horizontal axis, without relative movement on the vertical axis. This ensures that the cover plate remains locked onto the slide and does not separate. Compared to existing structures, an additional liquid replenishment position is added, allowing for sample replenishment without lifting the cover plate. This avoids the problem of dried slides and ensures that the entire reaction area is filled with replenishment liquid, solving problems such as inconsistent staining results and insufficient tissue repair.

[0019] Existing devices suffer from low heat dissipation efficiency, taking approximately 15 minutes to cool from around 103°C to 37°C, while this device only requires 7-8 minutes. This device's cooling and drainage system achieves heat dissipation for each heating block through independent air ducts corresponding to each heating block. The heating blocks feature heat dissipation fins to improve efficiency, and the air ducts form an internal circulation structure, avoiding uneven airflow and ensuring consistent heat dissipation across different heating blocks. Existing devices share a single air duct for waste liquid drainage and heat dissipation, making it easy for liquid to enter the heating and electrical components, leading to frequent component failures. This device separates the negative pressure air duct for waste liquid drainage from the heat dissipation channel for heating elements, and incorporates a waste liquid collection bottle, effectively preventing the risk of liquid entering the heating and electrical components.

[0020] In existing devices, when changing reagents at the reaction site, a cleaning solution needs to be added for cleaning, which mostly uses liquid displacement, resulting in low cleaning efficiency. This design uses a liquid cover plate to be dragged to the waste outlet. The movement of the cover plate cleans the reagent residue from the previous step before adding reagent, thus enhancing the cleaning effect. Furthermore, it relies on its own weight and the dual evacuation of a series booster fan and a vacuum pump to create negative pressure to assist in liquid waste discharge, thereby improving cleaning efficiency.

[0021] The pressure block assembly of this device ensures that the cover plate moves smoothly during operation and prevents occasional occurrences of cover plate jumping.

[0022] In existing devices, the installation of sub-module heating components either cannot be disassembled independently or only supports disassembly a few times. Subsequent replacements require the disassembly of most parts, making maintenance very cumbersome. This device can support disassembly without removing other components, thus providing better support for maintenance.

[0023] In commercially available devices, the reaction chamber is directly connected to the heat dissipation pipes or liquid lines. Disassembling the pipes is cumbersome and inconvenient for maintenance. This device separates the pipes from the main body, eliminating the need to disassemble the main body.

[0024] This device achieves six experimental operations through the cooperation of various structures in the drive assembly, including the initial position, locking position, liquid replenishment position, incubation position, sample addition position, and liquid discharge position, satisfying the entire process of IHC from slide baking to counterstaining. Attached Figure Description

[0025] Figure 1 This is a three-dimensional structural diagram of the device;

[0026] Figure 2 This is the front view of the device;

[0027] Figure 3 This is a side view of the device;

[0028] Figure 4 This is an exploded view of the device;

[0029] Figure 5 A top-view three-dimensional schematic diagram of the air duct base plate and the heating block fixing plate;

[0030] Figure 6 A three-dimensional schematic diagram of the air duct base plate and the heating block fixing plate viewed from below;

[0031] Figure 7 This is a schematic diagram of the heating block structure;

[0032] Figure 8 A schematic diagram of the structure of the side of the small cam with the first groove;

[0033] Figure 9A three-dimensional schematic diagram of the side of the large cam with the second groove;

[0034] Figure 10 This is a schematic diagram of the driver board structure;

[0035] Figure 11 This is a 3D view of the compaction block assembly;

[0036] Figure 12 This is a side view of the compaction assembly structure;

[0037] Figure 13 This is a schematic diagram of the compaction block structure;

[0038] Figure 14 This is a schematic diagram of the waste liquid collection bottle structure;

[0039] Figure 15 This is a schematic diagram of the initial bit state;

[0040] Figure 16 This is a schematic diagram of the locked position.

[0041] Figure 17 This is a schematic diagram showing the liquid level status.

[0042] Figure 18 This is a schematic diagram of the incubation site status;

[0043] Figure 19 This is a schematic diagram of the sample loading position.

[0044] Figure 20 This is a schematic diagram showing the liquid level status.

[0045] The diagram is labeled as follows: 1. Frame; 21. Waste liquid collection bottle; 211. Bottle body; 212. Guide pipe; 213. Airflow port; 22. Air duct base plate; 221. Negative pressure fan; 222. Negative pressure fan interface; 223. Heat dissipation outlet; 224. Waste liquid discharge port; 225. Drain outlet; 226. Air inlet; 23. Heating block fixing plate; 231. Rectangular tank; 2311. Drain hole; 2312. Air duct; 232. Ventilation drain rack; 2321. Heating tank; 2322. Heating block; 2322-1. Heating plate; 2322-2. Fins; 2322-3. Clip; 2322-4. Through hole; 3. Slide holder; 31. Slide; 4. Cover plate; 5. Motor; 6. Drive shaft. 61. Small cam; 611. First groove; 62. Support frame; 621. First roller; 63. Guide shaft; 64. Column guide shaft; 65. Guide rail; 66. Slide; 67. Large cam; 671. Second groove; 672. Fourth roller; 673. Fifth roller; 68. Drive plate; 681. Long groove; 682. Short groove; 683. Arc groove; 683-1. Vertical groove; 683-2. Arc groove; 69. Support plate; 691. Second roller; 692. Third roller; 7. Traction plate; 71. Traction piece; 8. Top cover; 81. Frame; 9. Pressure block assembly; 91. Pressure block mounting base; 92. Pressure block; 93. Pin; 94. Torsion spring; 95. Pressure block groove; 96. Limiting block. Detailed Implementation

[0046] To better explain and facilitate understanding of this utility model, the present utility model will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0047] like Figure 1-4 As shown, this utility model provides a fully automated device for tissue section repair and staining, comprising:

[0048] Frame 1;

[0049] A cooling and drainage system is installed on the frame 1; the cooling and drainage system includes an air duct base plate 22, a heating block fixing plate 23, a negative pressure fan 221, a waste liquid collection bottle 21, and a negative pressure fan interface 222, as shown below. Figure 1-2 As shown, the duct base plate 22 is mounted on the two opposing frames 1, and a heating block fixing plate 23 is mounted on the duct base plate 22, such as... Figure 6As shown, a heat dissipation outlet 223, a waste liquid discharge outlet 224, and a drain outlet 225 are provided in the middle of the air duct base plate 22. Multiple air inlets 226 are provided at the bottom of the air duct base plate 22. The heat dissipation outlet 223 is used to connect to a negative pressure fan 221, which employs a diagonal-flow centrifugal booster fan to provide a negative pressure environment for circulating air. The waste liquid discharge outlet 224 is connected to a negative pressure fan interface 222 via a waste liquid collection bottle 21. The negative pressure fan interface 222 is used to connect to a series booster fan. Preferably, the bottom of the waste liquid collection bottle 21 is connected to a waste liquid collection tank, which is also connected to a vacuum pump. The dual evacuation method of the series booster fan and the vacuum pump creates negative pressure to assist in liquid discharge, improving cleaning efficiency. The drain outlet 225 facilitates the removal of liquid that drips onto the heating block fixing plate 23 during the experiment.

[0050] Furthermore, such as Figure 14 As shown, the waste liquid collection bottle 21 includes a bottle body 211 and a guide tube 212. The guide tube 212 is located inside the bottle body 211, extending from the top to the bottom of the bottle body 211. The distance from the bottom of the bottle body 211 is approximately 1 / 5 to 1 / 4 of the height of the bottle body 211. An airflow port 213 is opened at the top of the bottle body 211. The guide tube 212 is connected to a waste liquid discharge port 224. The bottom of the guide tube 212 is set as an inclined opening, and the length of the opening is 1 / 3 to 1 / 2 of the length of the bottle body 211. The long opening facilitates the flow of liquid along the wall of the guide tube 212 into the bottom of the bottle body 211. The long opening length prevents it from being blocked by liquid and does not affect the negative pressure generated inside the guide tube 212 by the negative pressure airflow. The opening side is away from the airflow port 213. The airflow port 213 is connected to a negative pressure fan interface 222. The negative pressure fan interface 222 is connected to a series booster fan. The series booster fan provides negative pressure inside the bottle body 211 and the guide tube 212.

[0051] A heating block fixing plate 23 is installed above the air duct base plate 22, such as Figure 5-6 As shown, the heating block fixing plate 23 is a rectangular groove 231 with a ventilation drain rack 232. The upper surface of the ventilation drain rack 232 has multiple heating grooves 2321 and drain holes 2311, with each heating groove 2321 corresponding to a drain hole 2311. Heating blocks 2322 are placed inside the heating grooves 2321, and the number of heating grooves 2321 corresponds to the number of heating blocks 2322. The drain holes 2311 are connected to waste liquid outlets 224 on the ventilation duct bottom plate 22. During the experiment, waste liquid discharged from the cavity between each glass slide 31 and the cover plate 4 flows into the waste liquid collection bottle 21 through the drain holes 2311 to the waste liquid outlet 224 under the negative pressure created by the series booster fan and vacuum pump.

[0052] like Figure 5As shown, the inner wall of the ventilation drain rack 232 is provided with air ducts 2312 corresponding to the heating tank 2321. One end of the air duct 2312 corresponds to the air inlet 226 provided on the air duct bottom plate 22, and the other end of the air duct 2312 is located below the heating block 2322. When the negative pressure fan 221 starts working, the negative pressure formed will cause air to enter the corresponding air duct 2312 from each air inlet 226. Through the air duct 2312, air comes into contact with the fins 2322-2 of the corresponding heating block 2322 for heat exchange. The air enters the heat dissipation outlet 223, forming an air circulation, which evenly cools each heating block 2322. After 7-8 minutes, the temperature can be cooled from about 103℃ to 37℃. The negative pressure air duct for waste liquid discharge and the heat dissipation channel for heating elements are designed separately. Structurally, the heat dissipation air duct 2312 corresponds one-to-one with the heating tank 2321. The air duct forms an internal circulation structure. Heat dissipation of the heating device is achieved through independent air ducts, avoiding the problem of uneven air supply and improving the consistency and efficiency of heat dissipation of the heating blocks.

[0053] like Figure 1-4 As shown, a motor 5 is also installed below the air duct base plate 22. The motor 5 is connected to the drive shaft 6 through a worm gear. Both ends of the drive shaft 6 are connected to drive components.

[0054] like Figure 1-4 As shown, the drive assembly includes a small cam 61, a support frame 62, a guide shaft 63, a column guide shaft 64, a guide rail 65, a slide block 66, a large cam 67, a drive plate 68, and a support plate 69. The small cam 61 and the large cam 67 are coaxially fixed to the drive shaft 6, with the large cam 67 positioned outside the small cam 61. Figure 8As shown, a first groove 611 is provided on the outer circumference of the small cam 61. The first groove 611 cooperates with the first roller 621. The first roller 621 is set on the support frame 62. The two ends of the support frame 62 are slidably connected to the guide shaft 63. The guide shaft 63 is fixed to the frame 1. A guide rail 65 is fixed on the top of the support frame 62. The guide rail 65 is inclined, and the inclination angle is the same as the inclination angle of the heating block 2322. The end of the guide rail 65 is lower than the head direction of the cover plate 4, and the end is higher than the tail direction of the cover plate 4. A slide block 66 is slidably connected to the guide rail 65. The slide block 66 is an L-shaped plate. The short side of the L-shaped plate is fixedly connected to the drive plate 68, and the short side passes through the support plate 69. A drag plate 7 is fixedly connected to the long side of the L-shaped plate. The drag plate 7 is provided with multiple drag tabs 71 with openings at the top. The number of drag tabs 71 is the same as the number of heating blocks 2322. The opening of the drag plate 71 engages with the head of the cover plate 4. The end of the cover plate 4 with the protruding structure is the head, and the end with the liquid inlet is the tail. When the drive shaft 6 rotates, it drives the small cam 61 to rotate, which in turn drives the first roller 621 to rotate. The first roller 621 is fixed on the support frame 62. Under the limiting action of the guide shaft 63, the first roller 621 drives the support frame 62 to move up and down, thereby driving the drag plate 7 and the slide 66 to move up and down. The drag plate 7 can drive the cover plate 4 to perform dragging and lifting operations, with a lifting angle of 0 to 30°.

[0055] like Figure 9 As shown, a second groove 671 is provided on the side of the large cam 67 facing the small cam 61. The second groove 671 cooperates with the second roller 691, which is located at the bottom of the support plate 69. The support plate 69 has a frame structure, and its two ends are slidably connected to the column guide shaft 64. A spring is provided on the column guide shaft 64 to facilitate the stable movement and reset of the support plate 69. The top cover 8 is fixedly connected to the top of the support plate 69. A third roller 692 is also provided on the support plate 69, which cooperates with the drive plate 68.

[0056] like Figure 3 As shown, a fourth roller 672 and a fifth roller 673 are provided on the outer side of the large cam 67. Both the fourth roller 672 and the fifth roller 673 cooperate with the drive plate 68, as shown. Figure 10 As shown, the drive plate 68 is provided with a vertical long groove 681 and a short groove 682, as well as a bent arc-shaped groove 683. The bent arc-shaped groove 683 is a groove connecting the vertical groove 683-1 and the arc-shaped groove 683-2. The bent arc-shaped groove 683 restricts the slight horizontal movement of the drag plate 7 to achieve the sample replenishment function. The long groove 681 is located between the arc-shaped groove 683 and the short groove 682. The long groove 681 cooperates with the fourth roller 672, the arc-shaped groove 683 cooperates with the fifth roller 673, and the short groove 682 cooperates with the third roller 692.

[0057] Furthermore, a limiting component is provided above the support frame 62, located at the lower end of the guide rail 65. Specifically, the limiting component includes a spring and a damping positioning ball. A slot is formed above the support frame 62, and the spring is placed inside the slot. A damping positioning ball is positioned at the opening end of the spring and the slot. The damping positioning ball of the limiting component can contact the slide block 66, restricting its sliding along the inclined guide rail 65 when not under force constraint, ensuring stable cooperation between the drive plate 68 and its acting roller, and preventing mechanical jamming.

[0058] In summary, through the cooperation between the drive components, six experimental operations can be achieved, including the initial position, locking position, liquid replenishment position, incubation position, sample addition position, and liquid discharge position, satisfying the entire process of IHC from slide baking to counterstaining.

[0059] like Figure 1-2 As shown in Figure 4, the bottom ends of the slide holder 3 are provided with opposing sliding strips. The distance between the opposing sliding strips is slightly larger than the width of the ventilation drain rack 232. The sliding strips are engaged with the upper cover 8. Specifically, the upper cover 8 has L-shaped frames 81 on both sides. The slide holder 3 is engaged with the short side of the L-shape. The slide holder 31 is placed on the slide holder 3, so that the slides correspond one-to-one with the heating blocks, and can be easily inserted and removed. The end of the upper cover 8 (e.g. Figure 1 As shown on the right side, an elastic protrusion structure is provided on the inner side of the long side of the L-shape to position the slide holder 3, ensuring that the slides on the slide holder 3 correspond one-to-one with the heating block 2322. A cover plate 4 is placed above the slide 31, preferably the cover plate structure described in Chinese Patent CN214097474. This cover plate structure, combined with the slide, provides a large reaction area, facilitating sample preparation and helping to solve the problem of reagent evaporation and dry slides. Furthermore, the cover plate is provided with a liquid storage cavity (sample replenishment hole), which facilitates sample replenishment in the reaction cavity without lifting the cover plate, further avoiding the problem of dry slides. In the experiment, the slide 31 is placed above the heating block 2322 at an angle of 0 to 45° with the horizontal direction, preferably 5°. The side of the slide 31 relative to the tail of the cover plate 4 is higher, and the side relative to the head of the cover plate 4 is lower. This certain tilt angle facilitates the flow of liquid from the high end to the low end of the slide 31, making it easier to drain waste liquid from the slide 31 and fill the reaction solution.

[0060] like Figure 7As shown, the heating block 2322 includes a heating plate 2322-1, with fins 2322-2 fixed below the heating plate 2322-1. A through hole 2322-4 for placing a heating rod is opened between the fins 2322-2 and the heating plate 2322-1. The heating rod is placed in the through hole 2322-4. A clip 2322-3 is also provided below one end of the heating plate 2322-1 to fix the heating block 2322 in the drain hole 2311 of the heating block fixing plate 23, which facilitates draining and removal, is easy to disassemble, and better supports maintenance.

[0061] like Figure 1 As shown, the upper cover 8 has multiple T-shaped holes. The horizontal holes of the T-shaped holes are located at the tail of the cover plate 4, and the vertical holes of the T-shaped holes correspond one-to-one with the cover plate 4, which facilitates sample addition. Pressure block assemblies 9 are provided on both sides of the vertical holes of the T-shaped holes on the lower surface of the upper cover 8.

[0062] like Figure 11-13 As shown, the pressure block assembly 9 consists of a pressure block mounting base 91, at least one pressure block 92, a pin 93, and a torsion spring 94. The pressure block mounting base 91 has pressure block grooves 95, the same number as the pressure blocks 92. One end of each pressure block 92 is fixed to the pressure block groove 95 by the pin 93 and the torsion spring 94, while the other end of the pressure block 92 is used to press down on the cover plate 4. A limiting block 96 is provided within the pressure block groove 95. One side of the pressure block 92 contacts the limiting block 96, and the other side contacts the torsion spring 94. The limiting block 96 restricts the tilt angle of the pressure block 92, ensuring that during the waste liquid return stroke, the surface of the pressure block 92 facing the cover plate 4 is parallel to the inclined surface at the tail of the cover plate 4 (the inclined surfaces at the tails of both sides of the cover plate 4). The end of the pressure block 92 is a smooth arc surface R. The arc height H of the arc surface R is 0.5–1.5 mm. The cover plate 4 is an existing structure; refer to the cover plate structure described in Chinese Patent CN214097474.

[0063] In existing pressure block structures, during the waste discharge return process, the pressure block first contacts the inclined surface at the tail of the cover plate 4, with the point of downward pressure located to the right of the fulcrum. According to the lever principle, this will pry up the head of the cover plate 4, causing the cover plate 4 to spring up. The pressure block 92 structure designed in this application, when pushing the cover plate 4 back during drainage, has the side of the pressure block 92 facing the cover plate 4 parallel to the inclined surface at the tail of the cover plate 4. At this time, the pressure block 92 only contacts the inclined surface at the tail of the cover plate 4 but does not provide downward pressure until the arc surface of the end of the pressure block 92 contacts the inflection point between the upper surface of the cover plate 4 and the inclined surface. Only then does the pressure block 92 provide downward pressure on the cover plate 4. The contact point at this point is located to the left of the fulcrum (in the direction of the head of the cover plate 4), which is the point where the cover plate 4 contacts the glass slide when it is lifted. This avoids the lever phenomenon, and the cover plate 4 will not spring up.

[0064] This device achieves 6 operating positions, which are described in detail below in the order of rotation of the large cam 67:

[0065] Initial position: First, place the required glass slides 31 onto the slide holder 3 in sequence. Then, place the cover plate 4 on the glass slides 31 and slide the slide holder 3 into the frame 81 of the upper cover 8. At this time, each glass slide 31 corresponds to a heating block 2322 below and two pressure block assemblies above. The fifth roller 673 is at the end of the arc groove 683-2 of the arc groove 683, and the third roller 692 is located in the short groove 682 of the drive plate 68. The entire drive assembly is in the initial position, as shown below. Figure 15 As shown, this is the initial bit state or the reset origin.

[0066] Locked position: When the motor 5 drives the drive shaft 6 to rotate, the third roller 692 gradually disengages from the short groove 682 of the drive plate 68. The second groove 671 of the large cam 67 engages with the second roller 691. As the motor 5 rotates, the second roller 691 drives the support plate 69 to move downwards. The support plate 69 drives the pressure block assembly 9 to move downwards until it touches the upper surface of the top cover plate 4. The slide holder falls on the upper surface of the ventilation drain rack 232, and the slide 31 falls on the upper surface of the heating block 2322. The head of the cover plate 4 is engaged in the opening at the upper end of the drag strip 71. The fifth roller 673 moves in the arc groove 683-2 of the arc groove 683, moving to the other end of the arc groove 683-2, such as... Figure 16 As shown, this is the locked position. At this time, a relatively stable and closed reaction chamber is formed between the cover plate 4 and the glass slide 31.

[0067] Liquid replenishment status: Motor 5 continues to rotate, causing the fifth roller 673 to move within the vertical groove 683-1 of the arc-shaped groove 683. The fifth roller 673 moves slightly downwards relative to the locking position, such as... Figure 17 As shown, this is the liquid replenishment position. However, during experimental operation, typically, after the sample is added in the sample addition position, the motor 5 reverses to the incubation position, which is the liquid replenishment position. At this time, the cover plate 4 moves a certain displacement to the right (towards the tail of the cover plate 4) relative to the slide 31, and the sample replenishment hole is positioned at the upper edge of the reaction area of ​​the slide 31. This facilitates complete coverage of the reaction area by the replenishment liquid, avoiding varying degrees of dryness issues on the upper reaction area of ​​the slide in existing devices. During this process, the cover plate 4 and the slide 31 remain in a locked state, as shown... Figure 17 As shown, this is the state of replenishing the liquid level.

[0068] Incubation position status: When motor 5 continues to rotate, and the fifth roller 673 is located at the bottom of the vertical groove 683-1 of the arc-shaped groove 683, as follows... Figure 18 As shown, this is the incubation position.

[0069] The fifth roller 673 moves in the vertical groove 683-1 of the arc groove 683. While ensuring that the cover plate 4 is not lifted, it can move back and forth between the replenishment position and the incubation position. During the repair stage, there will be a large amount of reagent evaporation at the incubation position. The replenishment position can compensate for the amount of reagent evaporation, and compensate for the reagent without lifting the cover plate. This avoids the phenomenon of dry slices in the incubation experiment, which affects tissue activity, thereby ensuring that the tissue is fully repaired.

[0070] Sample loading position: Motor 5 continues to rotate, and the small cam 61 drives the first roller 621 on the support frame 62 to rotate, causing the support frame 62 to lift upwards. The lifting of the support frame 62 then causes the drag plate 7 to lift upwards, at which point the cover plate 4 lifts upwards. Subsequently, the fifth roller 673 of the large cam disengages from the arc-shaped groove 683, and the fourth roller 672 enters the long groove 681, driving the drive plate 68 to move. The drive plate 68 then drives the slide 66 to move on the guide rail 65, and the slide 66 drives the drag plate 7 to move. The drag plate 7 pulls the cover plate 4 towards the head. This results in the tail of the cover plate 4 being located within the reaction zone of the slide 31, preferably in the middle of the slide 31, and at a certain angle relative to the slide 31. Figure 19 As shown, this is the sample loading position.

[0071] The forward and reverse rotation of motor 5 enables the reciprocating motion from the incubation position to the sample application position. This action enables the cover plate 4 to be lifted, moved forward, and returned to its flat position, which can eliminate air bubbles generated when the cover plate 4 is pressed down and avoid false negatives. The repeated reciprocating motion is conducive to the uniform mixing of reagent concentration. The reagent added according to the capillary phenomenon will be more evenly applied to the tissue, ensuring the staining effect.

[0072] Liquid level status: Motor 5 continues to rotate, the fourth roller 672 moves downward along the long groove 681, continuing to drive the drive plate 68 to move. The drive plate 68 drives the slide 66 to continue moving on the guide rail 65. The slide 66 drives the drag plate 7 to continue moving until the drag plate 7 pulls the tail of the cover plate 4 to contact the head of the glass slide 31. At this time, the cover plate 4 carries the liquid scraped from the glass slide 31 to the corresponding drain hole 2311. Under the negative pressure formed by the series booster fan and vacuum pump, and the gravity effect of the glass slide 31 being tilted at a certain angle, the waste liquid flows through the drain hole 2311 to the waste liquid outlet 224 and into the waste liquid collection bottle 21, thereby enhancing the cleaning effect and improving the cleaning efficiency. Figure 20 As shown, this area is in the drain position.

[0073] The normal experimental operation sequence is: initial position, locking position, sample addition position, incubation position, liquid replenishment position, and liquid discharge position. The motor 5 can achieve reciprocating stepless motion by rotating in both directions, and can also switch between the above operation positions, depending on the experimental requirements.

[0074] In summary, this invention provides a fully automated device for tissue section repair and staining. Through the design of the drive plate structure, it achieves six experimental operations: initial position, locking position, liquid replenishment position, incubation position, sample addition position, and liquid discharge position. This automates the staining process and meets the requirements of IHC from slide baking to counterstaining. It effectively solves the problems of poor staining consistency, insufficient tissue repair, and dried slides found in existing devices.

[0075] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A fully automatic apparatus for tissue section mounting and staining, characterized by, The frame body (1) is provided with a cooling and liquid discharging system, the cooling and liquid discharging system is provided with a heating block (2322), the heating block (2322) is provided with a slide rack (3) above, the slide rack (3) is placed with a slide glass (31) above; the frame body (1) is further provided with a driving assembly, the driving assembly is connected with a pressing structure and a pulling plate (7), the pressing structure can press down a cover plate (4), the cover plate (4) and the slide glass (31) one-to-one correspond to form a reaction cavity, and the head of the cover plate (4) is connected with the pulling plate (7).

2. The fully automated apparatus for tissue section mounting and staining according to claim 1, wherein, The driving assembly comprises a driving plate (68), a small cam (61), a support frame (62), a guide shaft (63), a column guide shaft (64), a guide rail (65), a sliding seat (66), a large cam (67), a support plate (69), a first roller (621), a second roller (691), a third roller (692), a fourth roller (672) and a fifth roller (673), The guide shaft (63) and the column guide shaft (64) are arranged on the frame body (1), the support frame (62) is slidably connected to the guide shaft (63), the guide rail (65) is fixedly arranged above the support frame (62), the sliding seat (66) is slidably connected to the guide rail (65), the sliding seat (66) is an L-shaped plate, the short side of the L-shaped plate is fixedly connected with the driving plate (68) through the support plate (69), and the long side of the L-shaped plate is fixedly connected with the pulling plate (7); the first roller (621) is arranged on the support frame (62) and matched with the small cam (61); The support plate (69) is slidably connected to the column guide shaft (64), the pressing structure is fixedly connected to the top of the support plate (69), the second roller (691) and the third roller (692) are arranged on the support plate (69), the second roller (691) is matched with the large cam (67), and the third roller (692) is matched with the driving plate (68); the fourth roller (672) and the fifth roller (673) are also arranged on the large cam (67) and matched with the driving plate (68).

3. The fully automated apparatus for tissue section mounting and staining according to claim 2, wherein, The driving plate (68) is sequentially provided with a bent arc-shaped groove (683), a long groove (681) and a short groove (682).

4. The fully automated apparatus for tissue section mounting and staining according to claim 2, wherein The pressing structure comprises an upper cover (8) and a plurality of groups of pressing block assemblies (9) arranged below the upper cover (8).

5. The fully automated apparatus for tissue section mounting and staining according to claim 4, wherein The pressing block assembly (9) comprises a pressing block mounting seat (91), at least one pressing block (92), a pin (93) and a torsional spring (94), a plurality of pressing block grooves (95) are formed in the pressing block mounting seat (91) and correspond to the number of the pressing blocks (92), one end of the pressing block (92) is fixed in the pressing block groove (95) through the pin (93) and the torsional spring (94), and the other end of the pressing block (92) is used for pressing down the cover plate (4); a limiting block (96) is arranged in the pressing block groove (95), one side of the pressing block (92) abuts against the limiting block (96), the other side abuts against the torsional spring (94), the limiting block (96) limits the inclination angle of the pressing block (92), and the inclination angle satisfies that, when the waste liquid is discharged and returns, the surface of the pressing block (92) facing the cover plate (4) is parallel to the inclined surface of the tail of the cover plate (4).

6. The fully automated apparatus for tissue section mounting and staining according to claim 1, wherein, The cooling and liquid discharging system comprises a wind channel bottom plate (22), a heating block fixing plate (23), a negative pressure fan (221), a waste liquid collecting bottle (21) and a negative pressure fan interface (222), the wind channel bottom plate (22) is arranged on the frame body (1), the heating block fixing plate (23) is arranged above the wind channel bottom plate (22), the heat dissipation air outlet (223), the waste liquid discharge port (224), the water discharge port (225) and the air inlet (226) are arranged below the wind channel bottom plate (22), the heat dissipation air outlet (223) is used for connecting the negative pressure fan (221), and the waste liquid discharge port (224) is connected with the negative pressure fan interface (222) through the waste liquid collecting bottle (21).

7. The fully automated apparatus for tissue section mounting and staining according to claim 6, wherein The waste liquid collecting bottle (21) comprises a bottle body (211) and a flow guide pipe (212), the flow guide pipe (212) is arranged in the bottle body (211) and extends from the upper end of the bottle body (211) to the bottom of the bottle body (211), the air inlet (213) is arranged at the upper end of the bottle body (211), the flow guide pipe (212) is communicated with the waste liquid discharge port (224), and the air inlet (213) is communicated with the negative pressure fan interface (222).

8. The fully automated apparatus for tissue section mounting and staining according to claim 6, wherein The heating block fixing plate (23) is a structure that the ventilation and liquid discharge frame (232) is arranged on the rectangular groove body (231), a plurality of heating grooves (2321) and liquid discharge holes (2311) are arranged on the upper surface of the ventilation and liquid discharge frame (232), the heating grooves (2321) and the liquid discharge holes (2311) are one-to-one corresponding, the heating block (2322) is arranged in the heating groove (2321), the number of the heating grooves (2321) corresponds to the number of the heating blocks (2322), the liquid discharge holes (2311) are communicated with the waste liquid discharge ports (224) on the wind channel bottom plate (22), and the wind channel (2312) corresponding to the heating groove (2321) is arranged on the inner wall of the ventilation and liquid discharge frame (232), one end of the wind channel (2312) corresponds to the air inlet (226) arranged on the wind channel bottom plate (22), and the other end of the wind channel (2312) is located below the heating block (2322).

9. The fully automated apparatus for tissue section mounting and staining according to claim 1, wherein, The heating block (2322) comprises a heating plate (2322-1), the fin (2322-2) is fixed below the heating plate (2322-1), the through hole (2322-4) is arranged between the fin (2322-2) and the heating plate (2322-1), the heating rod is arranged in the through hole (2322-4), the clamping piece (2322-3) is further arranged below the end portion of one side of the heating plate (2322-1), and the clamping piece (2322-3) is clamped with the liquid discharge hole (2311) of the heating block fixing plate (23).

10. The fully automated apparatus for tissue section mounting and staining according to claim 1, wherein, A plurality of drag pieces (71) with open upper ends are arranged on the drag plate (7).

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