Concentration measuring device for high-anti-allergy mouse anti-human erythrocyte antibody

Abstract

The utility model relates to a high anti-allergy mouse anti-human red blood cell antibody concentration measuring device, which belongs to the technical field of biological experiment equipment and comprises a detection table, a containing groove is arranged at the left end of the upper surface of the detection table, a packaging box is arranged in an inner cavity of the containing groove, and a plurality of miniature reaction chambers are arranged in an inner cavity of the packaging box. A control panel is arranged at the right end of the detection table, the top of the micro reaction chamber is in threaded connection with a sealing cover, and a mixing input mechanism for mixing a sample and necessary reagents is arranged on the upper side of the sealing cover. According to the concentration measuring device for the high-anti-allergy mouse anti-human erythrocyte antibody, a sample and a necessary reagent can be uniformly mixed through the arranged mixing input mechanism before the sample enters the micro reaction chamber, so that the reaction uniformity is improved, and the result accuracy is ensured; meanwhile, by utilizing the arranged sealing mechanism, a worker can take out and pour out or add a reagent into a single micro reaction chamber, so that a plurality of samples are prevented from being polluted, and the practicability is improved.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment technology, specifically a device for measuring the concentration of highly sensitive mouse anti-human erythrocyte antibodies. Background Technology

[0002] Antibodies are protective proteins produced by the body in response to antigen stimulation. Measuring devices are required for detecting the concentration of mouse anti-human red blood cell antibodies. Currently, the most common method for measuring antibody concentration is to use specific reagent kits and compare the antibody concentration with a standard curve.

[0003] A search revealed Chinese Patent Publication No. CN220490682U, which discloses a device for measuring the concentration of mouse anti-human erythrocyte antibodies. The device includes a base, with a detection stage and a control console fixedly connected to the left and right sides of the top of the base, respectively. A control panel is fixedly embedded in the front surface of the control console. A shielding groove is formed on the front side of the top surface of the detection stage. Rotation notches are formed on both the left and right sides of the rear side of the inner cavity of the shielding groove. A covering device is installed inside the cavity of the rotation notch. A receiving groove is formed at the bottom of the inner cavity of the shielding groove, and the front side of the receiving groove is connected to the shielding notch. The utility model, through the combined use of a covering device, a supporting device, a shielding groove, a rotating notch, and a storage groove, has the advantage of shielding the detection area without the need for shielding components. During detection, contaminants from the external environment are less likely to enter the interior of the measuring solution, thus preventing contamination of the measuring solution and improving the accuracy of the concentration measuring device. However, the utility model lacks an automatic mixing function and cannot mix the sample with the necessary reagents evenly before injecting the sample into the storage container. This can lead to local concentrations that are too high or too low, resulting in uneven reaction of subsequent samples and reducing the accuracy of the measurement. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a high-sensitivity mouse anti-human erythrocyte antibody concentration measurement device. It has the advantages of being able to mix the sample with the necessary reagents evenly, thus solving the problem that the lack of automatic mixing function prevents the sample from being mixed evenly with the necessary reagents before injecting the sample into the storage vessel, which can lead to local concentrations that are too high or too low, resulting in uneven reaction of subsequent samples and reduced measurement accuracy.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a concentration measuring device for high-sensitivity mouse anti-human erythrocyte antibodies, comprising a detection platform, a receiving groove on the left end of the upper surface of the detection platform, a container inside the receiving groove, a plurality of miniature reaction chambers inside the container, a control panel on the right end of the detection platform, a sealing cap threaded to the top of the miniature reaction chamber, a mixing input mechanism for mixing samples and necessary reagents on the upper side of the sealing cap, and sealing mechanisms for sealing the miniature reaction chambers on both the left and right ends of the inner top wall of the sealing cap;

[0006] The mixing input mechanism includes a mixing cylinder, a pad, a locking block, two control valves, two guide pipes, a rotating rod, and two stirring blades. The mixing cylinder is located on the upper side of the sealing cover. The pad is fixed to the lower surface of the mixing cylinder, and the locking block is fixed to the lower surface of the pad. The two control valves are respectively fixed to the bottom of the left and right sides of the mixing cylinder. The guide pipes are connected to the water outlet of the control valves. The rotating rod is located in the inner cavity of the mixing cylinder, and the two stirring blades are fixed to the upper and lower ends of the outer surface of the rotating rod.

[0007] By adopting this technical solution, the mixing input mechanism can ensure that the sample is mixed with the necessary reagents before entering the micro-reaction chamber, thereby improving the uniformity of the reaction and ensuring the accuracy of the results.

[0008] Furthermore, a through hole is provided at the left end of the front of the testing platform. The size of the inner cavity of the through hole is adapted to the size of the container. A pull buckle is fixed on the front of the container. Multiple evenly distributed horizontal and vertical plates are fixed on the inner bottom wall of the container. Multiple spaces are formed between the horizontal and vertical plates. The micro reaction chamber is placed in the inner cavity of the space.

[0009] This technical solution facilitates the removal of the container.

[0010] Furthermore, guide blocks are fixed at both ends of the lower surface of the container, and guide grooves are provided at both ends of the bottom wall of the testing platform. The guide blocks move back and forth linearly within the inner cavity of the guide grooves.

[0011] By adopting this technical solution, the guide blocks and guide grooves can guide the movement of the container.

[0012] Furthermore, the top of the mixing cylinder is threaded with a cover plate, the upper surface of the rotating rod penetrates the cover plate and extends to the upper side of the cover plate and is fixed with a handle, and the two control valves are connected to the inner cavity of the mixing cylinder through water pipes on opposite sides.

[0013] By adopting this technical solution, samples can be added or stirring blades can be removed and cleaned by opening the cover.

[0014] Furthermore, the stirring blade includes a rotating sleeve and four rotating blades. The rotating sleeve is fixed to the outer surface of the rotating rod, and the four rotating blades are evenly fixed to the outer surface of the rotating sleeve.

[0015] Furthermore, the sealing mechanism includes two fixed boxes, two spring telescopic rods, two connecting blocks, two movable plates, two connecting rods, and a blocking plate. The two fixed boxes are respectively fixed to the left and right ends of the inner top wall of the sealing cover. The spring telescopic rods are located in the inner cavity of the fixed boxes and fixed to the inner top wall of the fixed boxes. The connecting blocks are fixed to the lower surface of the spring telescopic rods. The movable plates are fixed to the lower surface of the connecting blocks. The connecting rods are fixed to the upper surface of the movable plates. The upper surface of the sealing cover has a fixing hole. The blocking plate is located in the inner cavity of the fixing hole. The upper surfaces of the two connecting rods are fixed to the blocking plate.

[0016] By adopting this technical solution, the sealing mechanism allows staff to remove and pour out or add reagents to a single micro-reaction chamber, preventing multiple samples from being contaminated and improving practicality.

[0017] Furthermore, a slot is provided on the upper surface of the blocking plate, the inner diameter of the slot is adapted to the outer diameter of the blocking block, the inner diameter of the fixing hole is adapted to the outer diameter of the blocking plate, and the distance between the left side of the left end of the guide tube and the right side of the right end of the guide tube is less than the inner diameter of the fixing hole.

[0018] By adopting this technical solution, it is easy for two guide tubes to enter the inner cavity of the micro-reaction chamber.

[0019] Furthermore, the fixed box is a cuboid with a hollow interior and a missing lower surface. Guide rings are fixed on both the left and right sides of the fixed box, and guide rods that are slidably connected to the guide rings are fixed at both ends of the upper surface of the movable plate.

[0020] By adopting this technical solution, the guide ring and guide rod can limit the movement of the movable plate, enabling it to move stably in a straight line up and down.

[0021] Compared with the prior art, the technical solution of this application has the following beneficial effects:

[0022] This high-sensitivity mouse anti-human erythrocyte antibody concentration measurement device, through its mixing input mechanism, can uniformly mix the sample with necessary reagents before the sample enters the micro-reaction chamber, improving the uniformity of the reaction and ensuring the accuracy of the results. At the same time, the sealing mechanism allows staff to remove and pour out or add reagents to individual micro-reaction chambers, preventing multiple samples from being contaminated and improving practicality. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of this utility model;

[0024] Figure 2 This is a schematic diagram of the testing platform structure of this utility model;

[0025] Figure 3 This is a schematic diagram of the hybrid input mechanism of this utility model;

[0026] Figure 4 This is a schematic diagram of the sealing mechanism of this utility model.

[0027] In the diagram: 1. Testing platform; 2. Receiving tank; 3. Container box; 4. Miniature reaction chamber; 5. Control panel; 6. Sealing cover; 7. Mixing input mechanism; 71. Mixing cylinder; 72. Pad plate; 73. Clamping block; 74. Control valve; 75. Guide pipe; 76. Rotating rod; 77. Stirring blade; 8. Sealing mechanism; 81. Fixing box; 82. Spring telescopic rod; 83. Connecting block; 84. Movable plate; 85. Connecting rod; 86. Blocking plate. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] Please see Figures 1 to 2 This embodiment of a high-sensitivity mouse anti-human erythrocyte antibody concentration measuring device includes a detection stage 1. A receiving groove 2 is provided on the left end of the upper surface of the detection stage 1. A container 3 is provided inside the cavity of the receiving groove 2. Multiple micro-reaction chambers 4 are placed inside the cavity of the container 3. A control panel 5 is provided on the right end of the detection stage 1. A sealing cover 6 is threadedly connected to the top of the micro-reaction chamber 4. A mixing input mechanism 7 for mixing samples and necessary reagents is provided on the upper side of the sealing cover 6. Sealing mechanisms 8 for sealing the micro-reaction chambers 4 are provided on both the left and right ends of the inner top wall of the sealing cover 6.

[0030] Control panel 5 is equipped with a data processing module that receives data from the detection module and calculates antibody concentration according to a preset algorithm. The data processing module has an automatic correction function that can automatically correct the calculation parameters according to changes in environmental factors to improve the accuracy of measurement results.

[0031] In this embodiment, a through hole is provided on the left end of the front of the detection platform 1. The size of the inner cavity of the through hole is adapted to the size of the container 3. A pull buckle is fixed on the front of the container 3. Multiple evenly distributed horizontal and vertical plates are fixed on the inner bottom wall of the container 3. Multiple spaces are formed between the horizontal and vertical plates. The micro reaction chamber 4 is placed in the inner cavity of the space.

[0032] Guide blocks are fixed at both ends of the lower surface of the container 3, and guide grooves are provided at both ends of the bottom wall of the testing platform 1. The guide blocks move back and forth in a straight line in the inner cavity of the guide grooves. The guide blocks and guide grooves can guide the movement of the container 3.

[0033] It should be noted that a microfluidic chip is fixed to the inner bottom wall of the container 3 and located on the lower side of the micro-reaction chamber 4 for the specific binding of the sample and the antibody; the micro-reaction chamber 4 is equipped with an optical sensor for real-time monitoring of the reaction in the micro-reaction chamber 4; the micro-reaction chamber 4 is equipped with a temperature control system to precisely control the temperature in the micro-reaction chamber 4, so as to improve the efficiency and accuracy of the reaction.

[0034] Please see Figure 2 To ensure uniform mixing of the sample and necessary reagents, the mixing input mechanism 7 in this embodiment includes a mixing cylinder 71, a pad 72, a locking block 73, two control valves 74, two guide pipes 75, a rotating rod 76, and two stirring blades 77. The mixing cylinder 71 is located above the sealing cover 6. The cover plate threaded onto the upper surface of the mixing cylinder 71 is unscrewed, and then the sample and necessary reagents are poured into the inner cavity of the mixing cylinder 71. The pad 72 is fixed to the lower surface of the mixing cylinder 71, and the locking block 73 is fixed to the lower surface of the pad 72. The two control valves 74, 75, 76, and 77 are connected to the mixing cylinder 71. 4 are fixed to the bottom of the left and right sides of the mixing cylinder 71 respectively. The guide pipe 75 is connected to the water outlet of the control valve 74. The rotating rod 76 is located in the inner cavity of the mixing cylinder 71. Two stirring blades 77 are fixed to the upper and lower ends of the outer surface of the rotating rod 76. By rotating the rotating rod 76, the stirring blades 77 fixed on its surface will rotate, thereby mixing the sample and necessary reagents in the mixing cylinder 71. The sample and necessary reagents are automatically mixed evenly before the sample is injected, so as to improve the uniformity and accuracy of the reaction.

[0035] In this embodiment, the top of the mixing cylinder 71 is threaded with a cover plate. The upper surface of the rotating rod 76 passes through the cover plate and extends to the upper side of the cover plate, and a handle is fixed thereon. The two control valves 74 are connected to the inner cavity of the mixing cylinder 71 through water pipes on opposite sides. When the control valves 74 on both sides are opened, the sample mixture in the inner cavity of the mixing cylinder 71 can flow into the micro reaction chamber 4 through the guide tube 75. After the appropriate sample mixture is discharged, the control valves 74 can be closed until the sample in the guide tube 75 has completely flowed out.

[0036] The stirring blade 77 includes a rotating sleeve and four rotating blades. The rotating sleeve is fixed on the outer surface of the rotating rod 76, and the four rotating blades are evenly fixed on the outer surface of the rotating sleeve.

[0037] Please see Figure 3To prevent multiple samples from being contaminated, the sealing mechanism 8 in this embodiment includes two fixed boxes 81, two spring telescopic rods 82, two connecting blocks 83, two movable plates 84, two connecting rods 85, and a blocking plate 86. The two fixed boxes 81 are respectively fixed to the left and right ends of the inner top wall of the sealing cover 6. The spring telescopic rods 82 are located in the inner cavity of the fixed boxes 81 and are fixed to the inner top wall of the fixed boxes 81. The connecting blocks 83 are fixed to the lower surface of the spring telescopic rods 82. The movable plates 84 are fixed to the lower surface of the connecting blocks 83. The connecting rods 85 are fixed to the upper surface of the movable plates 84. The upper surface of the sealing cover 6 is provided with a fixing hole. The blocking plate 86 is located in the inner cavity of the fixing hole. The upper surfaces of the two connecting rods 85 are both fixed to the blocking plate 86.

[0038] In this embodiment, the spring telescopic rod 82 includes a spring and a telescopic rod. The telescopic rod consists of a sleeve and a moving rod. One end of the moving rod passes through and extends into the interior of the sleeve. A limiting block located inside the sleeve is fixedly connected to the outside of the moving rod. A through hole adapted to the moving rod is provided on one side of the sleeve. By providing the limiting block, the moving rod is prevented from detaching from the sleeve during movement.

[0039] The upper surface of the blocking plate 86 is provided with a slot, the inner diameter of which is adapted to the outer diameter of the blocking block 73, the inner diameter of the fixing hole is adapted to the outer diameter of the blocking plate 86, and the distance between the left side of the left end guide pipe 75 and the right side of the right end guide pipe 75 is less than the inner diameter of the fixing hole.

[0040] Understandably, when the card block 73 is placed in the slot opened on the upper surface of the blocking plate 86, and then the mixing cylinder 71 is pressed down, the mixing cylinder 71 can apply a downward force to the blocking plate 86, thereby causing the blocking plate 86 to move downward. The movement of the blocking plate 86 drives the connecting rod 85 to move downward, and the movement of the connecting rod 85 causes the movable plate 84 to move downward. The movement of the movable plate 84 drives the connecting block 83 to move downward, thereby causing the spring telescopic rod 82 to be stretched. At this time, the mixing cylinder 71 can move downward along with the movement of the blocking plate 86.

[0041] In this embodiment, the fixed box 81 is a cuboid with a hollow interior and a missing lower surface. Guide rings are fixed on both the left and right sides of the fixed box 81. Guide rods that are slidably connected to the guide rings are fixed on both the left and right ends of the upper surface of the movable plate 84. The guide rings and guide rods can limit the movement of the movable plate 84, so that it can move up and down in a straight line stably.

[0042] Understandably, once the sample in the guide tube 75 has completely flowed out, the mixing cylinder 71 can be pulled up. At this time, the spring telescopic rod 82 will rebound without pressure and drive the connecting block 83 and the movable plate 84 to move upward, thereby allowing the connecting rod 85 and the blocking plate 86 to move back into the inner cavity of the fixed hole, which can seal the inside of the micro reaction chamber 4. This allows the staff to take out and pour out or add reagents to a single micro reaction chamber 4, preventing multiple samples from being contaminated.

[0043] The working principle of the above embodiments is as follows:

[0044] When mixing the sample with the necessary reagents, the threaded cover plate on the upper surface of the mixing cylinder 71 can be unscrewed, and then the sample and necessary reagents can be poured into the inner cavity of the mixing cylinder 71. The cover plate can then be screwed back on. At this time, the rotating rod 76 can be rotated, causing the stirring blades 77 fixed on its surface to rotate, thus mixing the sample and necessary reagents in the mixing cylinder 71. After the sample and necessary reagents are evenly mixed, the locking block 73 can be placed in the slot on the upper surface of the blocking plate 86, and then the mixing cylinder 71 can be pressed down. The mixing cylinder 71 can apply a downward force to the blocking plate 86, causing the blocking plate 86 to move downward. The movement of the blocking plate 86 drives the connecting rod 85 to move downward, which in turn causes the movable plate 84 to move downward. The movement of the connecting block 83 causes it to move downward, which in turn stretches the spring telescopic rod 82. At this time, the mixing cylinder 71 can move downward along with the blocking plate 86 until the outlet end of the guide pipe 75 is located in the inner cavity of the micro reaction chamber 4. The control valves 74 on both sides can be opened, so that the sample mixture in the inner cavity of the mixing cylinder 71 can flow into the micro reaction chamber 4 along with the guide pipe 75. After the appropriate sample mixture is discharged, the control valve 74 can be closed. After the sample in the guide pipe 75 has completely flowed out, the mixing cylinder 71 can be pulled up. At this time, the spring telescopic rod 82 rebounds without pressure and drives the connecting block 83 and the movable plate 84 to move upward, so that the connecting rod 85 and the blocking plate 86 move back into the inner cavity of the fixed hole, which can seal the micro reaction chamber 4.

Claims

1. A device for measuring the concentration of highly sensitive mouse anti-human erythrocyte antibodies, comprising a detection stage (1), characterized in that: The left end of the upper surface of the detection platform (1) is provided with a receiving groove (2), the inner cavity of the receiving groove (2) is provided with a container (3), the inner cavity of the container (3) is provided with multiple micro reaction chambers (4), the right end of the detection platform (1) is provided with a control panel (5), the top of the micro reaction chamber (4) is threaded with a sealing cover (6), the upper side of the sealing cover (6) is provided with a mixing input mechanism (7) for mixing samples and necessary reagents, and the left and right ends of the inner top wall of the sealing cover (6) are provided with sealing mechanisms (8) for sealing the micro reaction chamber (4); The mixing input mechanism (7) includes a mixing cylinder (71), a pad (72), a locking block (73), two control valves (74), two guide pipes (75), a rotating rod (76), and two stirring blades (77). The mixing cylinder (71) is located on the upper side of the sealing cover (6). The pad (72) is fixed on the lower surface of the mixing cylinder (71). The locking block (73) is fixed on the lower surface of the pad (72). The two control valves (74) are respectively fixed at the bottom of the left and right sides of the mixing cylinder (71). The guide pipes (75) are connected to the water outlet of the control valves (74). The rotating rod (76) is located in the inner cavity of the mixing cylinder (71). The two stirring blades (77) are fixed at the upper and lower ends of the outer surface of the rotating rod (76).

2. The concentration measuring device for highly sensitive mouse anti-human erythrocyte antibodies according to claim 1, characterized in that: The detection platform (1) has a through hole on the left side of the front. The size of the through hole cavity is adapted to the size of the container (3). The front of the container (3) is fixed with a pull buckle. The inner bottom wall of the container (3) is fixed with multiple evenly distributed horizontal and vertical plates. Multiple spaces are formed between the horizontal and vertical plates. The micro reaction chamber (4) is placed in the cavity of the space.

3. The concentration measuring device for highly sensitive mouse anti-human erythrocyte antibodies according to claim 1, characterized in that: Guide blocks are fixed at both ends of the lower surface of the container (3), and guide grooves are provided at both ends of the bottom wall of the testing platform (1). The guide blocks move back and forth in a straight line within the guide groove.

4. The concentration measuring device for highly sensitive mouse anti-human erythrocyte antibodies according to claim 1, characterized in that: The top of the mixing cylinder (71) is threaded with a cover plate. The upper surface of the rotating rod (76) passes through the cover plate and extends to the upper side of the cover plate and is fixed with a handle. The two control valves (74) are connected to the inner cavity of the mixing cylinder (71) through water pipes on opposite sides.

5. The concentration measuring device for highly sensitive mouse anti-human erythrocyte antibodies according to claim 1, characterized in that: The stirring blade (77) includes a rotating sleeve and four rotating blades. The rotating sleeve is fixed on the outer surface of the rotating rod (76), and the four rotating blades are evenly fixed on the outer surface of the rotating sleeve.

6. The concentration measuring device for highly sensitive mouse anti-human erythrocyte antibodies according to claim 1, characterized in that: The sealing mechanism (8) includes two fixed boxes (81), two spring telescopic rods (82), two connecting blocks (83), two movable plates (84), two connecting rods (85), and a blocking plate (86). The two fixed boxes (81) are respectively fixed at the left and right ends of the inner top wall of the sealing cover (6). The spring telescopic rods (82) are located in the inner cavity of the fixed boxes (81) and fixed to the inner top wall of the fixed boxes (81). The connecting blocks (83) are fixed to the lower surface of the spring telescopic rods (82). The movable plates (84) are fixed to the lower surface of the connecting blocks (83). The connecting rods (85) are fixed to the upper surface of the movable plates (84). The upper surface of the sealing cover (6) is provided with a fixing hole. The blocking plate (86) is located in the inner cavity of the fixing hole. The upper surfaces of the two connecting rods (85) are fixed to the blocking plate (86).

7. The concentration measuring device for highly sensitive mouse anti-human erythrocyte antibodies according to claim 6, characterized in that: The upper surface of the blocking plate (86) is provided with a slot, the inner diameter of the slot is adapted to the outer diameter of the block (73), the inner diameter of the fixing hole is adapted to the outer diameter of the blocking plate (86), and the distance between the left side of the left end of the guide pipe (75) and the right side of the right end of the guide pipe (75) is less than the inner diameter of the fixing hole.

8. The concentration measuring device for highly sensitive mouse anti-human erythrocyte antibodies according to claim 6, characterized in that: The fixed box (81) is a cuboid with a hollow interior and a missing lower surface. Guide rings are fixed on both the left and right sides of the fixed box (81), and guide rods that are slidably connected to the guide rings are fixed on both the left and right ends of the upper surface of the movable plate (84).

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