In-vitro gene mutation test contamination device for gaseous or volatile substances
By designing the structure of the chamber, top cover, exhaust port, and storage compartment, the risk of gas leakage in the sealed operating chamber and the difficulty in retrieving and placing reagent plates were solved, achieving uniform gas contamination and safe and efficient gene mutation experiments.
Patent Information
- Application Number
- CN202422961468.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-03
- Publication Date
- 2025-12-02
- Estimated Expiration
- 2034-12-03
AI Technical Summary
Existing technologies for conducting in vitro gene mutation experiments on gaseous or volatile substances in sealed operating chambers present problems such as large space requirements, high gas demand, high risk of leakage, and difficulty in sterilization, making it difficult to handle reagent plates.
A device for in vitro gene mutation testing of gaseous or volatile substances was designed, comprising a box, a top cover, an exhaust port, an air inlet, and a storage compartment. It employs a structure including spring latches, sealing strips, a lifting part, and a damping push rod to achieve sealing and convenient reagent plate placement and removal.
Achieving gas pressurization and uniform contamination of reagent plates within a confined space solves the problem of difficulty in placing and removing reagent plates within a sealed structure, thus improving operational safety and efficiency.
Smart Images
Figure CN223615919U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of gene mutation, specifically to an in vitro gene mutation test exposure device for gaseous or volatile substances. Background Technology
[0002] According to the "Chemical Testing Methods: Health Effects Volume" published by the Chemical Registration Center of the Ministry of Environmental Protection, 2nd Edition, 2013, page 476, the in vitro mammalian cell gene mutation assay is an in vitro genetic toxicology assay that uses cultured mammalian cells as indicator organisms. It can be used to detect gene mutations induced by chemical substances. The most commonly used genetic endpoints are the detection of thymidine kinase (TK) markers, hypoxanthine-guanine transphosphoribokinase (HPRT / AGPRT), and xanthine transphosphoribokinase (XPRI). TK, HPRT / AGPRT~XPRI mutation assays can detect different genetic event profiles.
[0003] The principle of in vitro gene mutation assays is that under normal conditions, cells produce HPRT (hypoxanthine-guanine phosphoribosyltransferase). In a selective culture medium containing 6-thioguanine (6-TG), HPRT catalyzes the production of nucleoside-5-monophosphate (NVUP), which is incorporated into DNA, leading to cell death. Under the influence of carcinogens and / or mutagens, the structural gene controlling HPRT on the X chromosome of certain cells mutates, preventing the production of HPRT. This results in mutant cells being resistant to 6-TG and able to survive and grow in a selective culture medium containing 6-TG.
[0004] Key points of the experiment: With or without a metabolic activation system, cell cultures are exposed to the test substance for an appropriate time, and then the cells are passaged again. In a selective culture medium containing 6-TG, mutant cells will continue to divide and form colonies. The number of mutant colonies formed is counted, and the crossover frequency is calculated to infer the mutagenicity of the test substance.
[0005] However, conducting experiments directly in a sealed operating chamber is particularly inappropriate, as the internal space is large, requiring a large amount of gas and posing a risk of leakage. Furthermore, the gaseous or volatile substances are toxic, and subsequent disinfection is not easy to perform, thus requiring a specialized toxicity testing device. Utility Model Content
[0006] This application provides an in vitro gene mutation test device for gaseous or volatile substances, which solves the problems of the purpose of the test and the difficulty in handling the reagent plate. It achieves gas pressurization and easy handling of the reagent plate within a limited sealed structure.
[0007] The technical problem solved by this utility model can be achieved by the following technical solution:
[0008] An in vitro gene mutation assay device for a gaseous or volatile substance, comprising:
[0009] Box;
[0010] The top cover is detachably attached to the top of the housing;
[0011] The exhaust port is detachably connected to the outer wall of one side of the box and is connected to the inside of the box.
[0012] An air inlet is detachably connected to the upper surface of the top cover and is connected to the lower surface of the top cover.
[0013] Storage compartment, which is located inside the box.
[0014] Furthermore, a spring latch is detachably connected between the housing and the top cover, and each side of the housing has at least one centrally located spring latch.
[0015] Furthermore, the top of the housing has an insert groove, and a sealing strip is detachably connected inside the insert groove.
[0016] Furthermore, the storage unit includes:
[0017] A shelf, wherein the shelf is disposed inside the box;
[0018] The lifting unit is detachably connected to the shelf and is located inside the box.
[0019] Furthermore, the shelf has several air vents spaced at equal intervals.
[0020] Furthermore, the lifting unit includes two pairs of damping push rods, which are vertically fixed at the four corners of the shelf, with the shelf located at the center of the damping push rods.
[0021] Furthermore, the damping push rod is detachably connected to a top cap at both its upper and lower ends.
[0022] Furthermore, a guide block is detachably connected to the outside of the damping push rod, and the guide block has a prismatic structure.
[0023] The beneficial effects of this utility model are: by adopting a box body and top cover, the problem of uneven dyeing is effectively solved, thereby enabling experiments to be conducted under different requirements within a limited space.
[0024] By using a shelf in conjunction with a damping push rod, the problem of reagents easily tipping over when placed in and taken out due to the limited space inside the box is effectively solved, thus enabling the problem of placing and taking out reagents to be solved by using a height-adjustable shelf.
[0025] The use of a top cap effectively solves the problem of wear and tear on the casing and top cover, thereby protecting the surfaces of the casing and top cover.
[0026] By using guide blocks, the problem of centering the shelf is effectively solved, thus enabling self-adjustment through the guide blocks with a prismatic structure. Attached Figure Description
[0027] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0028] Figure 1 This is a schematic diagram of the structure of this utility model.
[0029] Figure 2 This is a schematic diagram of the damping push rod of this utility model.
[0030] Figure 3 This is a top view of the present invention.
[0031] In the diagram: 1-box body; 2-top cover; 3-exhaust port; 4-air inlet; 5-spring latch; 6-installation groove; 7-sealing strip; 8-shelf; 9-damping push rod; 10-top cap; 11-guide block. Detailed Implementation
[0032] Example 1:
[0033] Reference Figure 1-3 This is a schematic diagram of Embodiment 1 of the present invention, a device for in vitro gene mutation assay of gaseous or volatile substances, comprising:
[0034] Box 1;
[0035] Top cover 2, which is detachably connected to the top of the housing 1;
[0036] Exhaust port 3, which is detachably connected to the outer wall of one side of the housing 1 and is connected to the inside of the housing 1;
[0037] Air inlet 4 is detachably connected to the upper surface of the top cover 2 and is connected to the lower surface of the top cover 2.
[0038] Storage compartment, which is located inside the housing 1.
[0039] In actual use: First, place the reagent plate in the storage compartment, then connect the top cover 2 to the box body 1 to form a sealed mechanism between the box body 1 and the top cover 2. Then connect the air inlet 4 to the gas source and the exhaust port 3 to the waste gas system. Inject the pre-prepared test gas into the box body 1 through the air inlet 4, and at the same time open the exhaust port 3 to replace the gas in the box body 1. Then close the exhaust port 3 and continue to supply gas through the air inlet 4 to make the pressure conditions in the box body 1 reach the required level. Then let it stand for a certain period of time to complete the poisoning experiment.
[0040] In this embodiment, both the box body 1 and the top cover 2 are made of transparent material, so that the inside of the box body 1 can be seen from the outside.
[0041] When the top cover 2 is closed with the box body 1, the storage part enters the box body 1 at the same time.
[0042] Example 2:
[0043] Reference Figure 1-3 The difference in this embodiment is that: a spring buckle 5 is detachably connected between the box body 1 and the top cover 2, and each side of the box body 1 has at least one centrally located spring buckle 5.
[0044] In actual use: The top cover 2 can be easily closed and connected to the box body 1 by the spring buckle 5. At the same time, when opened, the top cover 2 can be completely separated from the box body 1. The spring buckle 5 can also keep the top cover 2 under downward pull when connected, so that the top cover 2 and the box body 1 are sealed.
[0045] Example 3:
[0046] Reference Figure 1-3 The difference in this embodiment is that: the top of the box 1 has an insert groove 6, and a sealing strip 7 is detachably connected in the insert groove 6.
[0047] In actual use: the sealing strip 7 can achieve the sealing between the top cover 2 and the box 1, and the mounting groove 6 can facilitate the positioning and replacement of the sealing strip 7.
[0048] Example 4:
[0049] Reference Figure 1-3 The difference in this embodiment is that the storage section includes:
[0050] Storage board 8, which is disposed inside the box body 1;
[0051] The lifting unit is detachably connected to the shelf 8 and is located inside the box 1.
[0052] In actual use: the reagent plate is placed on the shelf 8, and the shelf 8 can be moved down into the box 1 by the lifting part, thus solving the problem that the reagent plate is not easy to take out and put in the lower surface of the box 1.
[0053] Example 5:
[0054] Reference Figure 1-3 The difference in this embodiment is that the shelf 8 has several air holes at equal intervals.
[0055] In actual use: the gas flow rate can be increased through the vent, so that the contaminated gas can be evenly distributed after entering the chamber 1.
[0056] Example 6:
[0057] Reference Figure 1-3 The difference in this embodiment is that the lifting part includes two pairs of damping push rods 9, the two pairs of damping push rods 9 are vertically fixed at the four corners of the shelf 8, and the shelf 8 is located at the center of the damping push rods 9.
[0058] In actual use: Due to the use of damping push rod 9, the top cover 2 pushes the damping push rod 9, which in turn moves the placement plate 8 down into the box 1. At the same time, when the top cover 2 is removed, the placement plate 8 rises slowly under the action of the damping push rod 9, and the problem of sample overflow will not occur due to rapid rise.
[0059] Example 7:
[0060] Reference Figure 1-3 The difference in this embodiment is that the damping push rod 9 has a top cap 10 detachably connected to its upper and lower ends.
[0061] In actual use: the top cap 10 can increase the friction with the top cover 2, while reducing frictional damage to the bottom of the box 1.
[0062] Example 8:
[0063] Reference Figure 1-3 The difference in this embodiment is that a guide block 11 is detachably connected to the outside of the damping push rod 9, and the guide block 11 has a prismatic structure.
[0064] In actual use: the guide block 11 can automatically adjust and center the shelf 8 and damping push rod 9 when they are placed in.
[0065] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention, and all such changes are within the protection scope of the technology.
Claims
1. An in vitro gene mutation assay device for a gaseous or volatile substance, characterized in that, include: Box (1); Top cover (2), which is detachably connected to the top of the box body (1); The exhaust port (3) is detachably connected to the outer wall of one side of the box (1) and the exhaust port (3) is connected to the inside of the box (1); Air inlet (4), which is detachably connected to the upper surface of the top cover (2) and communicates with the lower surface of the top cover (2); Storage compartment, which is located inside the housing (1); The storage compartment includes: A shelf (8) is provided inside the box (1); The lifting part is detachably connected to the shelf (8) and is located inside the box (1); The lifting unit includes two pairs of damping push rods (9), which are vertically fixed at the four corners of the shelf (8), and the shelf (8) is located at the center of the damping push rods (9).
2. The in vitro gene mutation assay device for gaseous or volatile substances according to claim 1, characterized in that, A spring latch (5) is detachably connected between the box body (1) and the top cover (2), and each side of the box body (1) has at least one centrally located spring latch (5).
3. The in vitro gene mutation assay device for gaseous or volatile substances according to claim 1, characterized in that, The top of the box (1) has an insert groove (6), and a sealing strip (7) is detachably connected inside the insert groove (6).
4. The in vitro gene mutation assay device for gaseous or volatile substances according to claim 1, characterized in that, The shelf (8) has several air holes at equal intervals.
5. The in vitro gene mutation assay device for gaseous or volatile substances according to claim 1, characterized in that, The damping push rod (9) has a top cap (10) detachably connected to its upper and lower ends.
6. The in vitro gene mutation assay device for gaseous or volatile substances according to claim 1, characterized in that, The damping push rod (9) is detachably connected to a guide block (11) on its outer side, and the guide block (11) has a prismatic structure.