Light source standard device

Abstract

The utility model belongs to the field of analytical instrument measurement, and relates to a light source etalon, which is used for calibrating a microplate chemiluminescence analyzer, a calibration circular truncated cone comprises an upper layer cylinder, a middle layer cylinder and a lower layer cylinder, and the inner parts of the upper layer cylinder, the middle layer cylinder and the lower layer cylinder are hollow step structures of which the upper inner diameter is larger than the lower inner diameter; the bottom of the lower-layer cylinder is of a closed structure, a strip-shaped groove is formed in the upper surface of the closed structure of the bottom of the lower-layer cylinder, a light source device is placed in the strip-shaped groove, an inserting cylinder is integrally formed on the lower surface of the closed structure of the bottom of the lower-layer cylinder, and the periphery of the inserting cylinder is of a two-stage step structure. The outer diameter of the upper portion inserting connection cylinder is larger than that of the lower portion inserting connection cylinder, the outer diameter of the lower portion inserting connection cylinder is matched with the inner diameter of a hole site of the microwell plate, and square grooves are formed in the upper portions of the two opposite sides of the upper layer cylinder. The device is simple and convenient to operate, can realize calibration of all measurement hole sites, is compatible with circular and strip-shaped attenuation slices, and can save cost.

Description

TECHNICAL FIELD

[0001] The utility model belongs to analytical instrument measurement technical field, especially relate to photoelectric detection instrument measurement technology, concretely relate to a light source standard device for calibrating micro -hole board chemiluminescence analyzer. BACKGROUND

[0002] The micro -hole board chemiluminescence analyzer is a kind of instrument based on chemiluminescence phenomenon, using photoelectric detection technology, the light intensity produced by the reaction of a series of to-be-measured substances in micro -hole board and luminous reagent is detected and analyzed.

[0003] According to the micro -hole board chemiluminescence analyzer calibration specification, light source standard device mainly includes light source and spectral neutral filter, wherein the light source is composed of tritium light source and interference filter with peak wavelength 450nm, the light source can be placed in the arbitrary hole position of 96 -hole plate frame, the absorbance nominal value of spectral neutral filter is 0.2, 0.5, 1.0, 1.5 at 450nm respectively, spectral neutral filter is placed on the light source, and forms a set of light source measurement standard device.

[0004] Patent CN103234960A for micro -hole board chemiluminescence analyzer's measurement standard plate and its using method discloses a kind of measurement standard plate for micro -hole board chemiluminescence analyzer, including, load plate is equipped with uniformly distributed bearing hole, wherein:It further includes standard optical instrument fixed in bearing hole, the cavity of standard optical instrument consisting of light source component and optical instrument support includes coaxial sequentially installed tritium light source, light attenuation sheet, interference filter and neutral transmission ratio filter.The patent can carry out the representation of value accuracy, repeatability, sensitivity and channel difference, but it is difficult to calibrate the interference between holes. SUMMARY

[0005] To the technical problem of micro -hole board chemiluminescence analyzer interference between holes calibration, the utility model provides a kind of light source standard device for micro -hole board chemiluminescence analyzer calibration, can calibrate the indication deviation, indication repeatability, interference between holes, channel difference and linear error of micro -hole board chemiluminescence analyzer.The technical scheme that the utility model adopts is as follows:

[0006] The utility model relates to a light source standard ware, including calibration round table, the calibration round table is three layers of cylindrical structure, including integrative upper layer cylinder, middle layer cylinder and lower layer cylinder, the inside of upper layer cylinder, middle layer cylinder and lower layer cylinder is the hollow step structure of upper portion inner diameter is greater than lower portion inner diameter, the bottom of lower layer cylinder is the closed structure, and the upper surface of bottom closed structure of lower layer cylinder is provided with strip recess, and the light source device is placed in the strip recess, and the bottom closed structure lower surface of lower layer cylinder is integrative and is provided with the spigot cylinder, and the spigot cylinder is two stage step structure including upper portion spigot cylinder and lower portion spigot cylinder, and the outer diameter of upper portion spigot cylinder is greater than the outer diameter of lower portion spigot cylinder, and the outer diameter of lower portion spigot cylinder is matched with the hole site inner diameter of micro -hole plate, and the upper layer cylinder is provided with square recess above the opposite two sides.

[0007] Preferably, it further comprises a calibration plate, the shape of the calibration plate is consistent with the shape of the micro-hole plate, nine circular calibration positioning holes are formed on the calibration plate, the positions of the nine calibration positioning holes are one-to-one corresponding to the hole positions of the micro-hole plate required to be calibrated, and the outer diameter of the upper spigot cylinder is matched with the inner diameter of the calibration positioning hole.

[0008] Preferably, a raised frame is integrally formed on the lower surface of the calibration plate, and the height of the raised frame is greater than the height of the spigot cylinder.

[0009] Preferably, the upper layer cylinder is provided with a planar structure on the opposite two circumferential sides.

[0010] Preferably, a strip-shaped attenuating piece support is placed in the square recess of the upper layer cylinder, the strip-shaped attenuating piece support comprises a cuboid support shell, one end of the support shell is an open structure, a limiting protrusion is integrally formed on the open structure end, a mounting boss is arranged in the closed structure end opposite to the open structure end, the mounting boss is provided with a threaded hole in the vertical direction, and the two ends of the strip-shaped attenuating piece are respectively abutted on the inner sides of the mounting boss and the limiting protrusion; a through hole is formed in one end of the elastic metal sheet of the bending structure, and a fastening screw is screwed into the mounting boss through the through hole of the elastic metal sheet.

[0011] Preferably, a tritium lamp is placed in the strip recess as a light source, a narrow-band pass interference filter is placed on the circular step of the lower layer cylinder, and a circular attenuating piece is placed on the circular step of the upper layer cylinder.

[0012] Preferably, the narrow-band pass interference filter adopts an interference filter with a spectral bandwidth less than 15 nm, and the circular attenuating piece preferably adopts a neutral dark glass filter with a gradient absorbance value.

[0013] The utility model discloses the following beneficial effects:

[0014] The calibration round table of the utility model adopts multilayer cylinder structure, installs filter and attenuation piece through double-layer circular groove, takes the calibration plate with corresponding calibration positioning hole of general microwell plate as support, has the characteristics of simple operation and convenience, takes microwell plate as support, can realize calibration of all measurement hole positions, sets up square notch on the calibration round table, is compatible with common structure attenuation piece such as circular attenuation piece and strip-shaped attenuation piece, and can save cost by about one third. BRIEF DESCRIPTION OF DRAWINGS

[0015] The accompanying drawings are included to provide a further understanding of the utility model, and constitute a part of the specification, explain the utility model together with embodiments of the utility model, and do not constitute the limitation to the utility model.In the drawings:

[0016] Figure 1 It is the structure schematic diagram of the light source standarder of the utility model embodiment one;

[0017] Figure 2 It is the vertical section view of the calibration round table of the utility model embodiment one;

[0018] Figure 3 It is the vertical section view of the calibration round table of the utility model embodiment one and places calibration device;

[0019] Figure 4 It is the vertical section view of the calibration plate and calibration round table when calibrating of the utility model embodiment one;

[0020] Figure 5 It is the principle schematic diagram of the calibration plate and calibration round table when calibrating of the utility model embodiment one;

[0021] Figure 6 It is the schematic diagram of the strip-shaped attenuation piece support installed on the calibration round table of the utility model embodiment two;

[0022] Figure 7 It is the structure schematic diagram of the strip-shaped attenuation piece support of the utility model embodiment two;

[0023] Figure 8 It is the explosion view of the strip-shaped attenuation piece support of the utility model embodiment two;

[0024] Figure 9 It is the schematic diagram of the light source standarder of the utility model embodiment two and places on microwell plate;

[0025] In the figure, 1-calibration plate, 2-calibration round table, 3-calibration positioning hole, 4-weight reduction hole, 5-circular attenuation piece, 6-upper layer cylinder, 7-middle layer cylinder, 8-lower layer cylinder, 9-square groove, 10-strip-shaped groove, 11-inserting cylinder, 12-tritium lamp, 13-narrow band pass interference filter, 14-detector, 15-convex frame, 16-strip-shaped attenuation piece support, 17-strip-shaped attenuation piece, 18-support housing, 19-elastic metal sheet, 20-fastening screw, 21-mounting boss, 22-limiting bump, 23-microplate, 24-upper inserting cylinder, 25-lower inserting cylinder, 26-microplate D7 hole position. DETAILED DESCRIPTION

[0026] The utility model will be further described below by non-restrictive examples and in conjunction with the drawings. Example one

[0027] As Figure 1 shown, the utility model example one discloses a light source standard device, including calibration plate 1 and calibration round table 2.The appearance of calibration plate 1 is consistent with the appearance of general 96-hole microplate, and the size of calibration plate 1 is about 127mm*85mm*8mm, nine circular calibration positioning holes 3 and a plurality of rectangular weight reduction holes 4 are formed on calibration plate 1, the position of nine calibration positioning holes 3 respectively corresponds with the microplate hole position required by microplate chemiluminescence analyzer calibration specification one by one, the size of calibration positioning hole 3 is φ16mm, the inner diameter of nine calibration positioning holes 3 corresponds with the upper inserting cylinder 24 of calibration round table 2, the upper inserting cylinder 24 of calibration round table 2 is tightly inserted on calibration positioning hole 3 on calibration plate 1, and the circular attenuation piece 5 is placed in the upper layer cylinder 6 on the top of calibration round table 2. Figure 1 The position of calibration round table 2 inserted on calibration plate 1 is calibration plate D7 hole position in nine calibration positioning holes 3 on calibration plate 1, and calibration plate D7 hole position corresponds with microplate D7 hole position 26.

[0028] As Figure 2As shown, the calibration round table 2 is a three-layer cylindrical structure, including an integrally formed upper layer cylinder 6, a middle layer cylinder 7 and a lower layer cylinder 8. The interiors of the upper layer cylinder 6, the middle layer cylinder 7 and the lower layer cylinder 8 are hollow stepped structures. The bottom of the lower layer cylinder 8 is a closed structure. A strip-shaped groove 10 is arranged on the upper surface of the bottom closed structure of the lower layer cylinder 8. An insertion cylinder 11 is integrally formed on the lower surface of the bottom closed structure of the lower layer cylinder 8. The outer periphery of the insertion cylinder 11 is a two-stage stepped structure. The insertion cylinder 11 includes an upper insertion cylinder 24 and a lower insertion cylinder 25. The outer diameter of the upper insertion cylinder 24 is greater than the outer diameter of the lower insertion cylinder 25. The outer diameter of the upper insertion cylinder 24 is matched with the inner diameter of the calibration positioning hole 3. The outer peripheral surface of the insertion cylinder 11 is tightly inserted with the inner peripheral surface of the calibration positioning hole 3. Square grooves 9 are arranged on the upper layer cylinder 6 at opposite sides. Plane structures are machined on the opposite circumferential outer sides of the upper layer cylinder 6. The plane structures facilitate the installation and removal of the calibration round table 2. The axes of the grooves coincide with the axis of the calibration round table 2.

[0029] As shown in Figure 3 , when calibrating the microplate chemiluminescence analyzer, a tritium lamp 12 is placed in the strip-shaped groove 10 as a light source. A narrow-band interference filter 13 is placed on the circular step of the lower layer cylinder 8. A circular attenuation sheet 5 is placed on the circular step of the upper layer cylinder 6. The narrow-band interference filter 13 preferably adopts an interference filter with a spectral bandwidth less than 15 nm and a peak wavelength of 450 nm. The circular attenuation sheet 5 preferably adopts a neutral dark glass filter with a gradient absorbance value. The size of the neutral dark glass filter is φ20 mm. The absorbance nominal value of the neutral dark glass filter at a wavelength of 450 nm is 0.2, 0.5, 1.0, 1.5. The tritium lamp 12 and the narrow-band interference filter 13 can be installed by adhesion.

[0030] As shown in Figure 4 , 5 , after the upper insertion cylinder 24 of the calibration round table 2 is completely embedded in the calibration plate D7 hole position of the nine calibration positioning holes 3 of the calibration plate 1, the light source standard is placed in the sample chamber of the microplate chemiluminescence analyzer. The overall height h of the light source standard is between 14 mm and 25 mm. The circular cone top angle α formed by the tritium lamp 12 and the adjacent channel of the analyzer is included in the circular cone top angle β formed by the tritium lamp 12 and the narrow-band interference filter 13, that is, the radiation of the tritium light source is completely propagated to the adjacent channel of the detector 14 of the analyzer through the narrow-band interference filter 13 and the circular attenuation sheet 5.

[0031] A raised frame 15 is integrally formed on the lower surface of the calibration plate 1. The height of the raised frame 15 is greater than the height of the insertion cylinder 11 (the height of the upper insertion cylinder 24 + the height of the lower insertion cylinder 25). When calibrating the microplate chemiluminescence analyzer, the repeatability of the analyzer is calculated according to formula (1):

[0032] (1)

[0033] In the formula: -Luminous intensity The measurement value; - Average luminous intensity; - Number of repeated measurements; -Analyzer repeatability.

[0034] Calculate the inter-hole interference of the analyzer according to formula (2):

[0035] (2)

[0036] In the formula: -Inter-hole interference; -D7 hole surrounding the The measured value of the luminous intensity of the aperture; - Measurement of luminous intensity at aperture D7.

[0037] The upper insertion cylinder 24 of the calibration frustum 2 is inserted into the nine calibration plate holes 3 of the calibration plate 1 respectively, and the channel difference of the analyzer is calculated according to formula (3):

[0038] (3)

[0039] In the formula: -No. The measured value of luminous intensity from the aperture location; -The average value of the luminous intensity measurements from 9 apertures; - Differences in analyzer channels.

[0040] Insert the upper cylinder 24 of the calibration frustum 2 into the calibration plate D7 hole of the nine calibration positioning holes 3 on the calibration plate 1. Place the circular attenuators 5 with nominal absorbance values ​​of 0.2, 0.5, 1.0 and 1.5 on the circular step of the upper cylinder 6 in sequence, measure the absorbance value of the circular attenuators 5, and calculate the correspondence between the absorbance reading of the analyzer and the standard value according to formula (4):

[0041] (4)

[0042] In the formula: - The reading of the attenuation plate measured by the analyzer; - The correlation coefficient of the absorbance readings of the analyzer; - The standard value of absorbance for the attenuator.

[0043] Calculate the fitted value of the absorbance measured by the analyzer according to formula (5), and calculate the linearity error of the analyzer according to formula (6):

[0044] (5)

[0045] (6)

[0046] In the formula: - the linear error of the absorbance indicated value is - the fitting value of the absorbance indicated value - the absorbance measured value - the standard value of the absorbance of the attenuator. - the absorbance measured value - the standard value of the absorbance of the attenuator. Example Two

[0047] This example two is a calibration of the luminescence analyzer directly using the microplate 23 as a support. The common strip attenuator support 16 is used to install the strip attenuator 17. The strip attenuator support 16 is stably placed in the square groove 9 of the upper cylinder 6, and the light path formed by the tritium light source and the corresponding detector 14 completely passes through the strip attenuator 17.

[0048] As shown in Figures 6-9 , the strip attenuator support 16 includes a cuboid structure support shell 18. One end of the support shell 18 is an open structure, and a limiting lug 22 is integrally formed at the open structure end. An installation boss 21 is arranged inside the closed structure end opposite to the open structure end. The installation boss 21 is processed with a threaded hole. The two ends of the strip attenuator 17 are respectively abutted inside the installation boss 21 and the limiting lug 22. One end of the elastic metal sheet 19 is provided with a through hole. The fastening screw 20 passes through the through hole and is screwed into the installation boss 21, so as to firmly install the strip attenuator 17 in the support shell 18. The outer diameter of the lower insertion cylinder 25 matches the inner diameter of the hole position of the microplate 23.

[0049] According to the calibration specification of the microplate chemiluminescence analyzer, the lower insertion cylinder 25 of the calibration circular table 2 is embedded into the microplate D7 hole position 26 of the common microplate 23. The repeatability of the analyzer is calculated according to formula (1), and the inter-well interference of the analyzer is calculated according to formula (2). Then, the lower insertion cylinder 25 of the calibration circular table 2 is respectively embedded into the nine microplate hole positions required to be calibrated of the microplate 23. The channel difference of the analyzer is calculated according to formula (3).

[0050] The lower insertion cylinder 25 of the calibration circular table 2 is embedded into the microplate D7 hole position 26 of the microplate 23. The strip attenuator support 16 with the strip filter 17 having the absorbance nominal value of 0.2, 0.5, 1.0 and 1.5 is sequentially horizontally placed in the square groove 9. The linear error of the analyzer is calculated according to formula (4), (5) and (6).

[0051] The other parts are the same as example one.

[0052] The technical features not described in detail in the embodiments of the utility model are prior art or conventional technical means, which will not be described here.

[0053] Finally, it should be noted that: the above examples, only for the specific embodiments of the utility model, in order to illustrate the technical scheme of the utility model, rather than limit it, the protection scope of the utility model is not limited to this. Those skilled in the art should understand: any person skilled in the art in the technical range disclosed by the utility model, it can modify or easily think of changes to the technical scheme recorded in the foregoing examples, or equivalent replacement of part of the technical features; and these modifications, changes or replacement, without the essence of the corresponding technical scheme deviating from the spirit and scope of the technical scheme of the embodiments of the utility model, all should be covered in the protection scope of the utility model.

Claims

1. A light source standard comprising a calibration goniometer, characterized in that, The calibration circular table is a three-layer cylindrical structure, comprising an integrally formed upper cylinder, a middle cylinder and a lower cylinder, the interiors of the upper cylinder, the middle cylinder and the lower cylinder are hollow step structures with an upper inner diameter greater than a lower inner diameter, the bottom of the lower cylinder is a closed structure, the upper surface of the bottom closed structure of the lower cylinder is provided with a strip-shaped groove, a light source device is placed in the strip-shaped groove, the bottom closed structure of the lower cylinder is integrally formed with a plug-in cylinder, the plug-in cylinder is a two-step structure comprising an upper plug-in cylinder and a lower plug-in cylinder, the outer diameter of the upper plug-in cylinder is greater than the outer diameter of the lower plug-in cylinder, the outer diameter of the lower plug-in cylinder is matched with the inner diameter of the hole site of the microwell plate, and the upper cylinder is provided with square grooves above opposite sides.

2. A source meter according to claim 1, wherein, The calibration plate is further included, the shape of the calibration plate is consistent with the shape of the microwell plate, nine circular calibration positioning holes are formed on the calibration plate, the positions of the nine calibration positioning holes are one-to-one corresponding to the required calibration microwell plate hole sites, and the outer diameter of the upper plug-in cylinder is matched with the inner diameter of the calibration positioning hole.

3. A source meter according to claim 2, wherein, The lower surface of the calibration plate is integrally formed with a raised frame around the periphery, and the height of the raised frame is greater than the height of the plug-in cylinder.

4. A source meter according to claim 3, wherein, The upper cylinder is provided with a planar structure on opposite circumferential outer sides.

5. A source meter according to claim 1, wherein, The strip-shaped attenuation piece support is placed in the square groove of the upper cylinder, the strip-shaped attenuation piece support comprises a cuboid structure support shell, one end of the support shell is an open structure, the open structure end is integrally formed with a limiting protrusion, the closed structure end opposite to the open structure end is provided with a mounting boss inside, the mounting boss is processed with a vertical threaded hole, the two ends of the strip-shaped attenuation piece are respectively abutted on the inside of the mounting boss and the limiting protrusion, one end of the elastic metal sheet of the bending structure is provided with a through hole, and a fastening screw is screwed into the mounting boss through the through hole of the elastic metal sheet.

6. A source meter according to claim 1, wherein, A tritium lamp is placed in the strip-shaped groove as a light source, a narrow-band interference filter is placed on the circular step of the lower cylinder, and a circular attenuation piece is placed on the circular step of the upper cylinder.

7. A source meter according to claim 6, wherein, The narrow-band interference filter adopts an interference filter with a spectral bandwidth less than 15 nm, and the circular attenuation piece preferably adopts a neutral dark glass filter with a gradient absorbance value.

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