Carrier frame for cell crusher

Abstract

The utility model discloses a carrier for a cell crusher, the carrier for the cell crusher comprises a base and a carrier main body, the carrier main body is fixedly installed at the top end of the base, the carrier for the cell crusher comprises the base and the carrier main body, the carrier main body is fixedly installed at the top end of the base, and the carrier main body is fixedly installed at the top end of the base. A plurality of sample tube accommodating holes are formed in the carrier frame main body, the plurality of sample tube accommodating holes are arranged in a square matrix, gaps exist among the sample tube accommodating holes, and the sample tube accommodating holes are used for placing sample containers. The carrier for the cell disruption instrument is beneficial to control of temperature in the water tank, can rapidly dissipate heat to prevent sample denaturation caused by ultrasonic heat production in the cell disruption process, and is simple in structure, easy to manufacture, low in use cost, wide in application range and high in practicability.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology equipment, and in particular to a carrier for a cell disruptor. Background Technology

[0002] In high-throughput experiments of microbial cell culture and expression, cell disruption and lysis are currently key steps and essential procedures. Therefore, high-throughput ultrasonic disruption has emerged.

[0003] Currently, all multi-channel high-throughput cell disruptors on the market require the use of multi-well plates. The entire multi-well plate is embedded in the circulating water tank of the multi-channel cell disruptor to complete the disruption process. However, multi-well plates generally have no gaps in the middle, which leads to the generation of a large amount of heat during cell disruption. Due to the cohesive force of ultrasound, this heat may cause thermal denaturation of the sample, thereby affecting the cell disruption efficiency, experimental safety, and experimental accuracy.

[0004] Therefore, there is a need in the field for a cell disruptor carrier that can solve the heat dissipation problem in the circulating water tank of a cell disruptor, ensuring the safety of the experimental environment while improving cell disruption efficiency and experimental accuracy. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0006] A cell disruptor carrier includes a base and a carrier body. The carrier body is fixedly installed on the top of the base. The carrier body has multiple sample tube receiving holes arranged in a square array with gaps between them. The sample tube receiving holes are used to place sample containers.

[0007] In one specific embodiment, both the base and the main body of the carrier are cuboids, the bottom surface of the main body of the carrier coincides with the top surface of the base, the width of the carrier is greater than the width of the base, and the length of the carrier is less than the length of the base.

[0008] In one specific embodiment, the number of sample tube receiving holes is 6-24.

[0009] In a preferred embodiment, the number of sample tube receiving holes is 6, 12, or 24.

[0010] In one specific embodiment, the size of the gap is 3-7 mm.

[0011] In a preferred embodiment, the size of the gap is 3mm, 5mm or 7mm.

[0012] In one specific embodiment, the base is made of plexiglass, and the main body of the carrier is made of stainless steel.

[0013] In one specific embodiment, the sample container is a centrifuge tube.

[0014] In one specific embodiment, a silicone pad or an O-ring adapter is provided at the bottom of the base, the O-ring adapter being used to prevent the sample container from moving up and down.

[0015] The beneficial effects of this utility model include:

[0016] 1. The cell disruptor carrier provided by this utility model has multiple sample tube receiving holes on the main body of the carrier, which can be used to cooperate with the high-throughput operation of the multi-channel cell disruptor. At the same time, a gap of 3-7mm is set between the multiple sample tube receiving holes. The existence of this gap improves the water flow between the sample containers, which is conducive to good temperature control and prevents cell denaturation due to high heat in the water flow during the cell disruption process.

[0017] 2. The cell disruptor carrier provided by this utility model has a simple structure, is easy to manufacture, has low operating cost, and is widely applicable. For cell disruptors with different numbers of channels, the number of sample tube accommodating holes can be adjusted to improve the practicality of the new invention. Attached Figure Description

[0018] Figure 1 A schematic diagram of the structure of the cell disruptor frame provided by this utility model;

[0019] Figure 2 for Figure 1 Sectional view along line AA;

[0020] Figure 3 A side view of the carrier for the cell disruptor provided by this utility model.

[0021] In the diagram, 1 is the base; 2 is the main body of the carrier; 3 is the sample tube receiving hole; and 4 is the gap. Detailed Implementation

[0022] The technical solution of this utility model will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0023] Example 1

[0024] like Figure 1-3As shown in the figure, this embodiment illustrates a schematic diagram, side view, and cross-sectional view of the overall external structure of a cell disruptor carrier. The cell disruptor carrier includes a base 1 and a carrier body 2. The carrier body 2 is fixedly mounted on the top of the base 1. The carrier body 2 has multiple sample tube receiving holes 3 arranged in a square array, with gaps 4 between them. The sample tube receiving holes 3 are used to hold sample containers. In this embodiment, the gap 4 is 5mm in size, the number of sample tube receiving holes 3 is 24, arranged in a 4*6 square array, and the sample containers are 5mL centrifuge tubes.

[0025] Both the base 1 and the main body 2 are cuboids. The bottom surface of the main body 2 coincides with the top surface of the base 1. The width of the main body 2 is greater than the width of the base 1, and the length of the main body 2 is less than the length of the base 1. The base 1 and the main body 2 are not the same size, and the sides of the base 1 extend beyond the main body 2 to facilitate the operator's grip.

[0026] The base 1 is made of plexiglass, and the main body 2 of the cell disruptor is made of stainless steel. This material selection is appropriate and further enhances the heat dissipation of the cell disruptor's support frame. A silicone pad is installed at the bottom of the base 1, and an O-ring adapter is added to the sample tube receiving hole 3. The silicone pad increases the anti-slip properties of the base 1, and the O-ring adapter prevents the sample container from moving up and down, improving stability and safety during use. This prevents slippage during sample tube transfer, which could lead to sample tube spillage, and also avoids ultrasonic disruption failure caused by the sample container moving up and down during the cold water circulation in the ice bath.

[0027] The usage process of the cell disruptor frame shown in this embodiment is as follows:

[0028] The operator places the cell disruptor on the operating table using a carrier, and then places 5mL centrifuge tubes containing the cell solution to be disrupted into the sample tube receiving well 3. Afterwards, the operator holds both sides of the base 1 and transfers the cell disruptor to the circulating water tank of the multichannel cell disruptor for cell disruption and lysis.

[0029] In summary, the above embodiments are merely preferred embodiments of the present utility model and are not intended to limit the scope of protection of the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the scope of protection of the present utility model.

Claims

1. A carrier for a cell disruptor, characterized in that, The cell disruptor carrier includes a base and a carrier body. The carrier body is fixedly installed on the top of the base. The carrier body has multiple sample tube receiving holes arranged in a square array with gaps between them. The sample tube receiving holes are used to place sample containers.

2. The cell disruptor carrier according to claim 1, characterized in that, Both the base and the main body of the carrier are cuboids. The bottom surface of the main body of the carrier coincides with the top surface of the base. The width of the carrier is greater than the width of the base, and the length of the carrier is less than the length of the base.

3. The cell disruptor carrier according to claim 1, characterized in that, The number of sample tube receiving holes is 6-24.

4. The cell disruptor carrier according to claim 3, characterized in that, The number of sample tube receiving holes is 6, 12, or 24.

5. The cell disruptor carrier according to claim 1, characterized in that, The size of the gap is 3-7mm.

6. The cell disruptor carrier according to claim 5, characterized in that, The size of the gap is 3mm, 5mm or 7mm.

7. The cell disruptor carrier according to claim 1, characterized in that, The base is made of plexiglass, and the main body of the carrier is made of stainless steel.

8. The cell disruptor carrier according to claim 1, characterized in that, The sample container is a centrifuge tube.

9. The cell disruptor carrier according to claim 1, characterized in that, The bottom of the base is equipped with a silicone pad and an O-ring adapter.

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