WB electrophoresis comb capable of automatically loading samples
Patent Information
- Application Number
- CN202423028887.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-09
- Publication Date
- 2025-12-09
- Estimated Expiration
- 2034-12-09
AI Technical Summary
[0004]本实用新型的目的在于提供一种可自动上样的WB电泳梳，旨在解决实际操作过程中放置和拔出WB梳子过程中常因用力不均匀使得梳子与玻璃板之间存在空隙不一致，易导致加样时样本飘出加样孔、电泳拖尾、甚至电泳的时候样本跑不下去、溢出加样孔的问题
[0013] This utility model discloses an automatic sample loading WB electrophoresis comb. In use, a clamping assembly secures a fixing plate to the electrophoresis tank. The comb body is hung on the mounting plate. The controller extends the electric telescopic rod, causing the comb body to move downwards at a uniform speed until it reaches the appropriate position in the upper WB gel. The controller then pauses the operation, allowing the upper gel to solidify. Afterwards, the controller resumes operation, causing the electric telescopic rod to retract. The comb body is then slowly and uniformly pulled out of the upper gel. When it has been pulled halfway out, the operation is paused, and a 20µm sample is loaded. The barbell slowly adds samples and protein markers through the sample wells. After sample addition, the movement is controlled until the electrophoresis comb body is completely pulled out. The fixing plate and electrophoresis comb body are then removed to continue the subsequent WB operation. This application uses a controller to control the movement of the electric telescopic rod, which can achieve uniform speed as the electrophoresis comb body rises or falls. This solves the problem that uneven force often causes inconsistent gaps between the comb and the glass plate during the placement and removal of the WB comb in actual operation, which can easily lead to samples floating out of the sample well, electrophoresis tailing, or even samples not running down and overflowing during electrophoresis.
Smart Images
Figure CN223650496U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of electrophoresis comb technology, and in particular to a WB electrophoresis comb that can automatically load samples. Background Technology
[0002] A WB electrophoresis comb is a comb used in Western blotting experiments to prepare SDS-PAGE gels. It has multiple pores to form multiple sample loading slots in the gel, allowing multiple samples to be processed simultaneously in one gel.
[0003] However, in actual operation, uneven force is often applied during the placement and removal of the WB comb, resulting in inconsistent gaps between the comb and the glass plate. This can easily cause the sample to float out of the loading hole during loading, cause tailing during electrophoresis, or even prevent the sample from running down during electrophoresis and overflow the loading hole. Utility Model Content
[0004] The purpose of this invention is to provide an automatic WB electrophoresis comb, which aims to solve the problem that uneven force is often applied during the placement and removal of the WB comb in actual operation, resulting in inconsistent gaps between the comb and the glass plate. This can easily lead to the sample floating out of the loading hole, electrophoresis tailing, or even the sample not running down and overflowing during electrophoresis.
[0005] To achieve the above objectives, this utility model provides an automatic sample loading WB electrophoresis comb, including a fixing plate and a controller, wherein the controller is fixedly mounted on the fixing plate, and also includes auxiliary devices;
[0006] The auxiliary device includes an electric telescopic rod, a hanging plate, an electrophoretic comb body, and a clamping assembly. The electric telescopic rod is fixedly connected to the fixed plate and electrically connected to the controller, and is suspended below the fixed plate. The hanging plate is connected to the output end of the electric telescopic rod and is located on the output end of the electric telescopic rod. The electrophoretic comb body is hung on the hanging plate, and the clamping assembly is arranged on both sides of the fixed plate.
[0007] The electrophoresis comb body is transparent and visible, and has multiple cylindrical hollow holes arranged in a straight line from bottom to top, with sample loading holes connected to the cylindrical hollow holes;
[0008] The controller has a charging cable on the rear, a battery box and a control module inside, and an operating component on the top.
[0009] The clamping assembly includes an adjusting screw and an abutment plate. The adjusting screw is threadedly connected to the fixing plate and passes through the fixing plate, and is located on both sides of the fixing plate. The abutment plate is fixedly disposed on the end of the adjusting screw away from the fixing plate.
[0010] The operating components include a power button, a pause button, a sample dispensing port light button, and a lifting control component. The power button is electrically connected to the controller and fixed to the removable top cover of the controller; the pause button is electrically connected to the controller and fixed to the removable top cover of the controller; the sample dispensing port light button is electrically connected to the controller and fixed to the removable top cover of the controller; and the lifting control component is electrically connected to the controller and is located on the removable top cover of the controller.
[0011] The lifting control component includes an up control button and a down control button. The up control button is electrically connected to the controller and fixed to the removable cover plate on the top of the controller. The down control button is electrically connected to the controller and fixed to the removable cover plate on the top of the controller, and is located on the side of the controller closer to the up control button.
[0012] The controller also features a power indicator and a USB port on its top.
[0013] This utility model discloses an automatic sample loading WB electrophoresis comb. In use, a clamping assembly secures a fixing plate to the electrophoresis tank. The comb body is hung on the mounting plate. The controller extends the electric telescopic rod, causing the comb body to move downwards at a uniform speed until it reaches the appropriate position in the upper WB gel. The controller then pauses the operation, allowing the upper gel to solidify. Afterwards, the controller resumes operation, causing the electric telescopic rod to retract. The comb body is then slowly and uniformly pulled out of the upper gel. When it has been pulled halfway out, the operation is paused, and a 20µm sample is loaded. The barbell slowly adds samples and protein markers through the sample wells. After sample addition, the movement is controlled until the electrophoresis comb body is completely pulled out. The fixing plate and electrophoresis comb body are then removed to continue the subsequent WB operation. This application uses a controller to control the movement of the electric telescopic rod, which can achieve uniform speed as the electrophoresis comb body rises or falls. This solves the problem that uneven force often causes inconsistent gaps between the comb and the glass plate during the placement and removal of the WB comb in actual operation, which can easily lead to samples floating out of the sample well, electrophoresis tailing, or even samples not running down and overflowing during electrophoresis. Attached Figure Description
[0014] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.
[0015] Figure 1 This is a schematic diagram of the overall structure of the WB electrophoresis comb with automatic sample loading according to this utility model.
[0016] Figure 2 This is a cross-sectional view of the main body of the electrophoresis comb of this utility model.
[0017] In the diagram: 101-Fixing plate, 102-Controller, 103-Electric telescopic rod, 104-Hanging plate, 105-Electrophoresis comb body, 106-Cylindrical hollow hole, 107-Sample loading hole, 108-Charging cable, 109-Adjusting screw, 110-Abutting plate, 111-Power button, 112-Pause button, 113-Sample loading hole light button, 114-Up control button, 115-Down control button, 116-Power indicator, 117-USB port. Detailed Implementation
[0018] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.
[0019] like Figure 1 and Figure 2 As shown, where Figure 1 This is a schematic diagram of the overall structure of a WB electrophoresis comb with automatic sample loading. Figure 2 This is a cross-sectional view of the electrophoresis comb body 105. This utility model provides an automatically loading Western blotting (WB) electrophoresis comb, comprising a fixing plate 101, a controller 102, and auxiliary devices. The auxiliary devices include an electric telescopic rod 103, a hanging plate 104, the electrophoresis comb body 105, and a clamping assembly. The clamping assembly includes an adjusting screw 109 and an abutment plate 110. The operating components include a power button 111, a pause button 112, a sample loading port indicator button 113, and a lifting control component. The lifting control component includes an upward control button 114 and a downward control button 115. This solution solves the problem that uneven force during the placement and removal of the WB comb often results in inconsistent gaps between the comb and the glass plate, leading to sample overflowing from the loading port, electrophoresis tailing, or even sample not running down and overflowing during electrophoresis. The aforementioned solution allows the electrophoresis comb body 105 to rise or fall at a uniform speed, making it easier to operate.
[0020] In this embodiment, the controller 102 is fixedly mounted on the fixing plate 101, and the housing of the controller 102 is fixed to the fixing plate 101 by bolts.
[0021] The electric telescopic rod 103 is fixedly connected to the fixed plate 101 and electrically connected to the controller 102, and is suspended below the fixed plate 101. The hanging plate 104 is connected to the output end of the electric telescopic rod 103 and is located on the output end of the electric telescopic rod 103. The electrophoretic comb body 105 is hung on the hanging plate 104. The clamping assembly is set on both sides of the fixed plate 101. The mounting plate on the top of the housing of the electric telescopic rod 103 is fixed to the fixed plate 101 by bolts. The hanging plate 104 is fixed to the output end of the electric telescopic rod 103 by bolts with countersunk bolts. Hanging posts are respectively set on both sides of the hanging plate 104 so that the hanging holes on both sides of the top of the electrophoretic comb body 105 can be slidably inserted to realize the installation of the electrophoretic comb body 105. The clamping assembly facilitates the fixing plate 101 to be fixed on electrophoresis tanks of different sizes.
[0022] The electrophoresis comb body 105 is transparent and visible, with multiple cylindrical hollow holes 106 arranged in a straight line from bottom to top, and sample loading holes 107 connected to the cylindrical hollow holes 106; the sample loading holes 107 are used for sample loading, and the electrophoresis comb body 105 is made of polycarbonate and styrene copolymer.
[0023] The controller 102 has a charging cable 108 on its rear side, a battery box and a control module inside, and an operating component on its top. One end of the charging cable 108 has a plug for easy power connection, and the other end can be directly plugged into the charging port of the controller 102 to charge the battery in the internal battery box. The control module uses a RI SC microprocessor, and the operating component facilitates operation and control.
[0024] Secondly, the adjusting screw 109 is threadedly connected to the fixing plate 101, passes through the fixing plate 101, and is located on both sides of the fixing plate 101; the abutment plate 110 is fixedly disposed on the end of the adjusting screw 109 away from the fixing plate 101. Rectangular frames are provided on both sides of the fixing plate 101, and through threaded holes are provided on the side walls of the rectangular frames to facilitate direct installation of the adjusting screw 109. The adjusting screw 109 has a T-shaped cross-section, with a turntable on one side and the abutment plate 110 fixedly connected to the other side by bolts, with the bolts countersunk.
[0025] Then, the power button 111 is electrically connected to the controller 102 and fixed to the removable cover plate on the top of the controller 102; the pause button 112 is electrically connected to the controller 102 and fixed to the removable cover plate on the top of the controller 102; the sample inlet light button 113 is electrically connected to the controller 102 and fixed to the removable cover plate on the top of the controller 102; the lifting control component is electrically connected to the controller 102 and is disposed on the removable cover plate on the top of the controller 102. The power button 111 is used for power on and off control of the controller 102, the pause button 112 is used to control the electric telescopic rod 103 to stop its movement, and the lifting control component is used to control the lifting and lowering movement of the electric telescopic rod 103.
[0026] Furthermore, the upward control button 114 is electrically connected to the controller 102 and fixed to the detachable top cover of the controller 102; the downward control button 115 is electrically connected to the controller 102 and fixed to the detachable top cover of the controller 102, and is located on the side of the controller 102 near the upward control button 114. The upward control button 114 is used to control the electrophoretic comb body 105 to move upward, and the downward control button 115 is used to control the electrophoretic comb body 105 to move downward.
[0027] Finally, the top of the controller 102 is also provided with a power display 116 and a USB port 117. The power display 116 is used to display the power level, and the USB port 117 facilitates the connection of a USB data cable.
[0028] When using this invention to address the problem that uneven force often results in inconsistent gaps between the comb and the glass plate during the placement and removal of the WB comb in actual operation, leading to sample overflowing from the loading hole, electrophoresis tailing, or even sample not running down and overflowing during electrophoresis, the following steps are taken: Place the fixing plate 101 on top of the electrophoresis tank. Then, rotate the adjusting screws 109 on both sides to move the abutment plate 110 and abut it against the side walls of the electrophoresis tank, thus fixing the fixing plate 101 to the electrophoresis tank. Hang the electrophoresis comb body 105 on the hanging plate 104. Click the power button 111, then click the descent control button 115 to extend the electric telescopic rod 103. The electrophoresis comb body 105 will then move downwards at a constant speed until it reaches the appropriate position in the upper WB gel. Then press the pause button 112. After the upper gel solidifies, click the ascend control button 114 to retract the electric telescopic rod 103. The electrophoresis comb body 105 will then slowly and uniformly pull out of the upper gel. When it has pulled out halfway, press the pause button 112, then click the sample loading port light button 113. Then use a 20u... The sample and protein marker are slowly added through the sample loading port 107 using a barbell. After the sample is added, the up control button 114 is pressed until the electrophoresis comb body 105 is completely pulled out. The clamping assembly is then released, the fixing plate 101 and the electrophoresis comb body 105 are removed, and the subsequent WB operation is completed. This application controls the electric telescopic rod 103 through the controller 102 to achieve uniform speed up or down of the electrophoresis comb body 105. This solves the problem that uneven force often causes inconsistent gaps between the comb and the glass plate during the placement and removal of the WB comb in actual operation, which can easily lead to the sample floating out of the loading port, electrophoresis tailing, or even the sample not running down and overflowing the loading port during electrophoresis.
[0029] The above-disclosed embodiments are merely one or more preferred embodiments of this application and should not be construed as limiting the scope of this application. Those skilled in the art can understand that all or part of the processes for implementing the above embodiments and equivalent changes made in accordance with the claims of this application still fall within the scope of this application.
Claims
1. A WB electrophoresis comb capable of automatic sample loading, comprising a fixing plate and a controller, wherein the controller is fixedly mounted on the fixing plate, characterized in that, It also includes auxiliary devices; The auxiliary device includes an electric telescopic rod, a hanging plate, an electrophoretic comb body, and a clamping assembly. The electric telescopic rod is fixedly connected to the fixed plate and electrically connected to the controller, and is suspended below the fixed plate. The hanging plate is connected to the output end of the electric telescopic rod and is located on the output end of the electric telescopic rod. The electrophoretic comb body is hung on the hanging plate, and the clamping assembly is arranged on both sides of the fixed plate. The electrophoresis comb body is transparent and visible, and has multiple cylindrical hollow holes arranged in a straight line from bottom to top, with sample loading holes connected to the cylindrical hollow holes; The controller has a charging cable on the rear, a battery box and a control module inside, and an operating component on the top.
2. The WB electrophoresis comb with automatic sample loading as described in claim 1, characterized in that, The clamping assembly includes an adjusting screw and an abutment plate. The adjusting screw is threadedly connected to the fixed plate and passes through the fixed plate, and is located on both sides of the fixed plate. The abutment plate is fixedly disposed on the end of the adjusting screw away from the fixed plate.
3. The WB electrophoresis comb with automatic sample loading as described in claim 1, characterized in that, The operating components include a power button, a pause button, a sample dispensing port light button, and a lifting control component. The power button is electrically connected to the controller and fixed to the removable top cover of the controller; the pause button is electrically connected to the controller and fixed to the removable top cover of the controller; the sample dispensing port light button is electrically connected to the controller and fixed to the removable top cover of the controller; and the lifting control component is electrically connected to the controller and is located on the removable top cover of the controller.
4. The WB electrophoresis comb with automatic sample loading as described in claim 3, characterized in that, The lifting control component includes an up control button and a down control button. The up control button is electrically connected to the controller and fixed to the removable cover plate on the top of the controller. The down control button is electrically connected to the controller and fixed to the removable cover plate on the top of the controller, and is located on the side of the controller closer to the up control button.
5. The WB electrophoresis comb with automatic sample loading as described in claim 1, characterized in that, The controller also has a power indicator and a USB port on its top.