Nucleic acid detection module and sample analyzer

Abstract

The embodiment of the utility model provides a nucleic acid detection module and a sample analyzer, the nucleic acid detection module comprises a bearing assembly, an amplification module, a plurality of optical fibers and an optical fiber limiting assembly, the amplification module comprises a temperature control unit and a heat conduction part, the heat conduction part comprises a plurality of tube parts, the plurality of tube parts are arranged along a first direction, and the tube wall of each tube part is provided with at least two insertion holes; one insertion hole of the same tube part is used for inserting the end part of an excitation optical fiber, and the other insertion hole is used for inserting the end part of a receiving optical fiber; the optical fiber limiting assembly is provided with at least one wire fixing groove, and the multiple optical fibers extend towards the first side of the amplification module in the first direction in the direction away from the pipe part and penetrate through the at least one wire fixing groove. According to the nucleic acid detection module provided by the embodiment of the invention, the occupied space of the plurality of optical fibers is small, and the layout compactness of the optical fibers and the accommodating cavity is high. The jack can be tightly attached to the heat conduction part even through simple insertion, displacement caused by vibration or other external force is avoided, and the influence of the end of the optical fiber on the temperature of the heat conduction part is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of in vitro diagnosis, and in particular to a nucleic acid detection module and a sample analyzer. BACKGROUND

[0002] Taking a sample analyzer as a polymerase chain reaction (PCR) instrument as an example, a detection device utilizes optical fibers to make a to-be-tested liquid contained in an amplification container arranged in a reaction cavity generate a fluorescent signal under excitation, so as to reflect the material characteristics of the to-be-tested liquid, thereby performing qualitative or quantitative analysis. However, in the related technology, the optical fibers and the reaction cavity are dispersedly arranged, which leads to a large occupied space, so that the overall volume of a single amplification module is large, which is not conducive to the miniaturization design of the sample analyzer; and the end of the optical fiber is fixed to the reaction cavity by adhesion or other complex methods, which is not convenient for disassembly and maintenance, and the adhesive and the like will affect the temperature of the reaction cavity, thereby affecting the reaction reliability. CONTENT OF THE UTILITY MODEL

[0003] Therefore, an embodiment of the present application aims to provide a nucleic acid detection module and a sample analyzer, the layout of optical fibers and a containing cavity is highly compact, and the end of the optical fiber has little effect on the temperature of the reaction cavity.

[0004] An embodiment of the present application provides a nucleic acid detection module, comprising:

[0005] a bearing assembly;

[0006] an amplification module arranged in the bearing assembly, the amplification module comprising a temperature control unit and a heat conduction component, the temperature control unit being configured to provide heat and / or cold to the heat conduction component, the heat conduction component comprising a plurality of tube portions arranged along a first direction, the tube portions having containing cavities for containing amplification containers loaded with samples, and tube walls of the tube portions being provided with at least two insertion holes;

[0007] a plurality of optical fibers, the plurality of optical fibers comprising a plurality of excitation optical fibers and a plurality of receiving optical fibers, one of the insertion holes of the same tube portion being configured to allow the end of the excitation optical fiber to be inserted, and the other insertion hole being configured to allow the end of the receiving optical fiber to be inserted;

[0008] an optical fiber limiting assembly arranged in the bearing assembly and located on a first side of the heat conduction component in the first direction, the optical fiber limiting assembly being provided with at least one wire fixing groove, and the plurality of optical fibers extending towards the first side of the amplification module in the first direction away from the tube portions and passing through the at least one wire fixing groove.

[0009] In some embodiments, the extension direction of the wire fixing groove is parallel to the first direction.

[0010] In some embodiments, the plurality of excitation fibers are located on a first side of a reference plane, the plurality of receiving fibers are located on a second side of the reference plane, the first side and the second side are opposite to each other, and the reference plane is a plane in which the center lines of the plurality of tube portions are located.

[0011] In some embodiments, the fiber limiting assembly provides a tensioning force to the plurality of fibers to allow the ends of the plurality of fibers to be detachably abutted against the corresponding jacks to fix the ends of the fibers relative to the jacks.

[0012] In some embodiments, the amplification module further comprises a compression assembly and at least one first connecting member.

[0013] The heat conduction component comprises a tube base, the tube base is in heat conduction with the temperature control unit, and the plurality of tube portions are connected to a side of the tube base away from the temperature control unit.

[0014] The compression assembly comprises a pressing plate and at least one column structure, the pressing plate and the at least one column structure are connected, the column structure is at least partially arranged between the pressing plate and the tube base, the first connecting member connects the pressing plate and the bearing assembly, and the compression assembly compresses the tube base through the column structure; and an end of the plurality of fibers close to the heat conduction component is arranged between the pressing plate and the tube base.

[0015] In some embodiments, at least part of the plurality of fibers are arranged around one or more sides of the column structure away from the tube portions to allow the column structure to assist in tensioning the fibers.

[0016] In some embodiments, the first connecting member has a plurality of first portions and a plurality of second portions, the first portions and the second portions are located on opposite sides of the plurality of tube portions in a second direction, and an end of the plurality of fibers close to the heat conduction component is located between the first portions and the second portions.

[0017] The second direction, the first direction, and a height direction of the nucleic acid detection module are perpendicular to each other.

[0018] In some embodiments, the fiber limiting assembly comprises a first clamping portion and a second clamping portion, the first clamping portion is arranged on the bearing assembly, the first clamping portion and / or the second clamping portion are provided with the at least one wire fixing groove, and the first clamping portion and the second clamping portion are detachably connected and clamp the plurality of fibers.

[0019] In some embodiments, an outer periphery of the fiber is provided with a hard structure, and the first clamping portion and the second clamping portion clamp the hard structure to fix the fiber.

[0020] In some embodiments, the rigid structure comprises a metal ring, the metal ring encircles an outer periphery of the optical fiber.

[0021] In some embodiments, a step surface of a slot wall of the wire slot faces the heat conduction component, the rigid structure has an abutting surface, the abutting surface and the step surface abut in the first direction.

[0022] In some embodiments, the number of the wire slots is plural, and the plural wire slots are arranged along a second direction;

[0023] A single wire slot accommodates one optical fiber; or, a single wire slot accommodates plural optical fibers, the step surface extends along a height direction of the nucleic acid detection module, the plural optical fibers accommodated in the same wire slot are stacked along the height direction of the nucleic acid detection module and abut the step surface;

[0024] Wherein, the second direction, the first direction, and the height direction of the nucleic acid detection module are perpendicular to each other.

[0025] In some embodiments, the first clamping part comprises a first connecting sub-part, a second connecting sub-part, and a third connecting sub-part arranged at intervals in the second direction, a spacing region between the first connecting sub-part and the second connecting sub-part forms a first groove, and a spacing region between the second connecting sub-part and the third connecting sub-part forms a second groove.

[0026] The first clamping part further comprises a plurality of spacing walls, part of the spacing walls are arranged in the first groove to separate the first groove into a plurality of wire slots, and another part of the spacing walls are arranged in the second groove to separate the second groove into a plurality of wire slots.

[0027] Wherein, the plurality of excitation optical fibers pass through the plurality of wire slots of the first groove, and the plurality of receiving optical fibers pass through the plurality of wire slots of the second groove.

[0028] The optical fiber limiting assembly comprises a plurality of second connecting members, and the second clamping part is connected to the first connecting sub-part, the second connecting sub-part, and the third connecting sub-part through the plurality of second connecting members, respectively.

[0029] In some embodiments, the bearing assembly comprises a bottom plate and a support structure arranged on the bottom plate, and the amplification module is arranged on the support structure, wherein at least part of the support structure is configured as a heat dissipation structure for dissipating heat for the temperature control unit.

[0030] The nucleic acid detection module comprises a cover, an open side of the cover is formed, opposite ends of the cover in a first direction are respectively provided with openings, the cover is arranged on the outer periphery of the amplification module and the support structure, the open side of the cover abuts against the bottom plate, and one of the openings is used for allowing the plurality of optical fibers to pass through; and the other opening is used for at least allowing a cable to pass through, and the cable is used for at least electrically connecting with the temperature control unit.

[0031] The sample analyzer provided in the embodiments of the present application comprises:

[0032] The nucleic acid detection module is the nucleic acid detection module in any of the embodiments of the present application.

[0033] The light emitting module is configured to provide excitation light through the excitation optical fiber.

[0034] The light receiving module is configured to receive the optical signal transmitted by the receiving optical fiber and convert the received optical signal into an electrical signal.

[0035] The control module is configured to acquire the electrical signal and obtain a sample detection result according to the electrical signal.

[0036] In some embodiments, the sample analyzer comprises a bearing table, and the number of the nucleic acid detection modules is multiple, and the multiple nucleic acid detection modules are arranged on the bearing table and are arranged at intervals along a second direction.

[0037] The second direction intersects the first direction and is perpendicular to the height direction of the sample analyzer.

[0038] The nucleic acid detection module provided in the embodiments of the present application has the following advantages. The plurality of optical fibers are extended towards the first side of the amplification module in the first direction away from the heat conduction component, the distance between the plurality of optical fibers is small, the plurality of optical fibers can be collected and fixed through the wire fixing groove in the case that each accommodation cavity corresponding to the plurality of optical fibers is not deformed or has small deformation, the distance between the optical fibers is reduced during fixing, the occupied space of the plurality of optical fibers is small, the layout of the optical fibers and the accommodation cavities is compact, meanwhile, the extension length of the optical fibers in the direction perpendicular to the first direction is small, the overall volume of the nucleic acid detection module is reduced, and thus the miniaturization design of the nucleic acid detection module is facilitated. Meanwhile, the end of the optical fiber away from the tube is fixed through the wire fixing groove, the end of the optical fiber can be simply plugged into the jack to realize close adhesion with the heat conduction component, displacement of the end of the optical fiber due to vibration or other external force is avoided, the structure and transmission reliability are improved, complex fixing is not needed, disassembly is facilitated, and the influence of the end of the optical fiber on the temperature of the heat conduction component is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1A schematic view of a part of a sample analyzer according to an embodiment of the present application;

[0040] Figure 2 A schematic view of a nucleic acid detection module according to an embodiment of the present application;

[0041] Figure 3 A schematic view of a part of a sample analyzer according to an embodiment of the present application; Figure 2 A schematic view of a part of a sample analyzer according to an embodiment of the present application;

[0042] Figure 4 A schematic view of a part of a sample analyzer according to an embodiment of the present application; Figure 2 A schematic view of a part of a sample analyzer according to an embodiment of the present application;

[0043] Figure 5 A schematic view of a part of a sample analyzer according to an embodiment of the present application; Figure 2 A schematic view of a part of a sample analyzer according to an embodiment of the present application;

[0044] Figure 6 A schematic view of a part of a sample analyzer according to an embodiment of the present application; Figure 5 A schematic view of a part of a sample analyzer according to an embodiment of the present application;

[0045] Figure 7 A schematic view of a part of a sample analyzer according to an embodiment of the present application; Figure 6 A schematic view of a part of a sample analyzer according to an embodiment of the present application;

[0046] Figure 8 A schematic view of a part of a sample analyzer according to an embodiment of the present application; Figure 6 A schematic view of a part of a sample analyzer according to an embodiment of the present application;

[0047] Figure 9 A schematic view of a part of a sample analyzer according to an embodiment of the present application.

[0048] Legend of reference signs

[0049] 100 - amplification module; 200 - bearing table; 300 - optical fiber; 301 - excitation optical fiber; 302 - receiving optical fiber; 400 - hard structure; 400a - abutting surface; 500 - cable;

[0050] 11 - heat conducting component; 111 - tube base; 112 - tube part; 112a - accommodating cavity; 112b - insertion hole;

[0051] 12 - optical fiber limiting assembly; 12a - wire fixing groove; 12b - step surface; 121 - first clamping part; 121a - first recess; 121b - second recess; 1211 - first connecting sub-part; 1212 - second connecting sub-part; 1213 - third connecting sub-part; 1214 - spacing wall; 122 - second clamping part; 123 - second connecting piece;

[0052] 13 - bearing assembly; 131 - bottom plate; 132 - support structure; 14 - cover; 14a - opening; 14b - opening;

[0053] 15-pressing assembly; 151-pressing plate; 152-column structure; 16-first connecting member; 161-first part; 162-second part. DETAILED DESCRIPTION

[0054] In order to make the objects, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and do not limit the present application.

[0055] In the specific embodiments, various specific technical features described can be combined in any appropriate manner without contradiction, for example, different embodiments and technical solutions can be formed by combining different specific technical features. In order to avoid unnecessary repetition, various possible combinations of various specific technical features in the present application are not described again.

[0056] In the following description, the terms "first\second\..." are only used to distinguish different objects, and do not mean that the objects have the same or relationship. It should be understood that the orientation description "upper", "lower", "outer", "inner" is the orientation in the normal use state, and the "left" and "right" directions represent the left and right directions shown in the specific corresponding schematic diagram, which can be the left and right directions in the normal use state or not.

[0057] It should be noted that the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or device. Without more limitation, the element defined by the sentence "including one" does not exclude the existence of other identical elements in the process, method, article or device including the element. "Multiple" means greater than or equal to two.

[0058] The embodiment of the present application provides a nucleic acid detection module.

[0059] In the embodiment of the present application, the nucleic acid detection module is applied to a sample analyzer.

[0060] Please refer to Figures 1 to 9 , the nucleic acid detection module includes a bearing assembly 13, an amplification module 100, a plurality of optical fibers 300 and an optical fiber limiting assembly 12.

[0061] The bearing assembly 13 is used to provide mounting support for the setting of the amplification module 100, the plurality of optical fibers 300 and the optical fiber limiting assembly 12.

[0062] The amplification module 100 is arranged on the bearing assembly 13, the amplification module 100 comprises a temperature control unit and a heat conducting component 11, the temperature control unit is used to provide heat and / or cold to the heat conducting component 11, the heat conducting component 11 comprises a plurality of tube portions 112, the plurality of tube portions 112 are arranged along a first direction, the tube portion 112 has a containing cavity 112a for containing the amplification container loaded with the sample, and the tube wall of the tube portion 112 is provided with at least two insertion holes 112b.

[0063] Here, the amplification module 100 is a device component for performing a nucleic acid amplification reaction, exemplarily, for a polymerase chain reaction, which can amplify a trace amount of nucleic acid in a nucleic acid extraction liquid formed after nucleic acid extraction on a liquid containing a sample to a sufficient amount for subsequent analysis and detection.

[0064] The temperature control unit is the core component of the amplification module 100, which can accurately control the temperature change, so as to support the temperatures required by the denaturation stage, the annealing stage and the extension stage of the amplification reaction such as the polymerase chain reaction.

[0065] The heat conducting component 11 is used to transmit the heat and / or cold generated by the temperature control unit to the amplification container containing the nucleic acid extraction liquid through the containing cavity 112a, and the plurality of containing cavities 112a arranged along the first direction can accommodate a plurality of amplification containers, so that each nucleic acid extraction liquid is subjected to the same temperature treatment, ensuring the consistency of the reaction temperature.

[0066] The number of insertion holes 112b can be two or more, which is not limited here.

[0067] Please refer to Figure 5 and Figure 7 , the plurality of optical fibers 300 comprises a plurality of excitation optical fibers 301 and a plurality of receiving optical fibers 302, one of the insertion holes 112b of the same tube portion 112 is used for inserting the end of the excitation optical fiber 301, and the other insertion hole 112b is used for inserting the end of the receiving optical fiber 302.

[0068] That is, one containing cavity 112a corresponds to one excitation optical fiber 301 and one receiving optical fiber 302.

[0069] The excitation optical fiber 301 can transmit excitation light to make the sample in the amplification container have a fluorescence reaction and generate a fluorescence signal, the excitation light can come from the light emitting module of the sample analyzer, the receiving optical fiber 302 can transmit the fluorescence signal to the light receiving module of the sample analyzer, and the fluorescence signal is converted into an electric signal by the light receiving module, so that the control module of the sample analyzer can obtain the sample detection result according to the electric signal.

[0070] Here, the optical fiber limiting assembly 12 is used to fix the position of the optical fiber 300 to increase the position reliability of the optical fiber 300, facilitate stable excitation light transmission and fluorescence signal transmission, and thus increase the detection reliability.

[0071] Please refer to Figures 3 to 5 The optical fiber limiting assembly 12 is arranged on the bearing assembly 13 and located on the first side of the heat conduction component 11 in the first direction. The optical fiber limiting assembly 12 is provided with at least one wire fixing groove 12a. The plurality of optical fibers 300 extend towards the first side of the amplification module 100 in the first direction away from the tube part 112 and pass through the at least one wire fixing groove 12a.

[0072] It should be noted that the plurality of optical fibers 300 extend towards the first side of the amplification module 100 in the first direction away from the heat conduction component 11, which means that the plurality of optical fibers 300 extend in the spacing direction of the plurality of accommodating cavities 112a. In this way, on the one hand, the end of the plurality of optical fibers 300 cooperates with the jack 112b, and the other end extends towards the first side of the first direction away from the heat conduction component 11. The distance between the optical fibers 300 is relatively small, that is, the optical fibers 300 cooperating with different accommodating cavities 112a can be conveniently gathered and fixed as a whole through the wire fixing groove 12a, the occupied space of the plurality of optical fibers 300 is reduced, and the deformation of the optical fiber 300 is small, which will not affect the setting reliability of the optical fiber 300 and damage the optical fiber 300. On the other hand, the arrangement length of the amplification module 100 perpendicular to the first direction can be reduced, thereby facilitating the reduction of the overall size of the amplification module 100.

[0073] It should be noted that the extension of the optical fiber 300 on the first side of the first direction means that the whole optical fiber 300 extends towards the first side of the first direction, that is, a small amplitude offset relative to the first direction is allowed.

[0074] Exemplarily, taking the length direction of the amplification module 100 as the first direction, the arrangement size of the amplification module 100 in the width direction can be reduced.

[0075] Here, the plurality of optical fibers 300 pass through the at least one wire fixing groove 12a. When the number of wire fixing grooves 12a is one, the plurality of optical fibers 300 are fixed through one wire fixing groove 12a. In this way, the structure of the optical fiber limiting assembly 12 can be relatively simple. When the number of wire fixing grooves 12a is multiple, the plurality of optical fibers 300 are fixed through multiple wire fixing grooves 12a. In this way, the limiting ability of the plurality of optical fibers 300 can be improved, the size requirement of a single wire fixing groove 12a is reduced, and the wire fixing reliability is high.

[0076] It can be understood that in the related art, the optical fibers extend along the direction perpendicular to the spacing direction of the accommodation cavities. On the one hand, the distances between the optical fibers corresponding to each group of accommodation cavities are large, the optical fibers are arranged dispersedly, and a large space is occupied. In addition, a large fixing area is required, and meanwhile, the overall occupied area of the amplification module is large, which is not convenient for miniaturization design of the sample analyzer. Meanwhile, the end of the optical fiber is fixed to the tube portion by adhesion or other complex methods, which is not convenient for disassembly. In addition, the adhesives and the like will affect the temperature of the heat conduction component, and affect the reliability of the amplification reaction.

[0077] The nucleic acid detection module provided by the embodiments of the present application can be used to extend the plurality of optical fibers 300 in the direction away from the heat conduction component 11 towards the first side of the amplification module 100 in the first direction. The distance between the plurality of optical fibers 300 is small, and the plurality of optical fibers 300 can be folded and fixed through the wire fixing groove 12a without deformation or with small deformation of the plurality of optical fibers 300 corresponding to each accommodation cavity 112a. The distance between the plurality of optical fibers 300 is reduced during fixing, the occupied space of the plurality of optical fibers 300 is small, the layout of the optical fibers 300 and the accommodation cavities 112a is compact, and meanwhile, the extension length of the optical fibers 300 in the direction perpendicular to the first direction is small, which can reduce the overall volume of the nucleic acid detection module, thereby facilitating miniaturization design of the nucleic acid detection module. Meanwhile, the end of the optical fiber 300 can be simply inserted into the jack 112b through the simple insertion of the end of the optical fiber 300, and then the end of the optical fiber 300 can be tightly attached to the heat conduction component 11, without displacement due to vibration or other external forces, thereby increasing the transmission reliability, without complex fixing, facilitating disassembly, and reducing the influence of the end of the optical fiber 300 on the temperature of the heat conduction component 11.

[0078] For the amplification reaction, the temperature needs to be controlled during the reaction process to ensure the accuracy, uniformity and temperature control speed of the temperature. Once the temperature of the heat conduction component 11 is affected, the amplification efficiency is easily reduced, the amount of amplification product is affected, and even the amplification fails, which seriously affects the efficiency and accuracy of the nucleic acid detection.

[0079] In some embodiments, referring to Figures 3 to 5 , all the accommodation cavities 112a of a single amplification module 100 are arranged in one-dimensional linear arrangement.

[0080] Here, the one-dimensional linear arrangement refers to that all the accommodation cavities 112a of a single amplification module 100 are arranged along a straight line or a single row. In this way, the mechanical design and manufacturing process can be simplified, and the structural complexity is reduced. The linear arrangement can also utilize the limited space to reduce the volume of the amplification module 100. Of course, all the accommodation cavities 112a are arranged on a straight line, which is convenient for realizing consistent temperature control, reducing temperature difference, making each sample under the same thermal cycling condition, and increasing detection reliability. In addition, the optical fibers 300 are also more convenient to arrange and fix.

[0081] In some embodiments, referring to Figures 3 to 6 , the extending direction of the wire fixing groove 12a is parallel to the first direction. In this way, the end of the optical fiber 300 away from the tube part 112 is fixed from the extending direction of the optical fiber 300, reducing the probability of unnecessary bending of the optical fiber 300 when fixed by the wire fixing groove 12a, and increasing the wire fixing reliability.

[0082] In some embodiments, referring to Figure 5 , the plurality of excitation optical fibers 301 are located on a first side of a reference plane, the plurality of receiving optical fibers 302 are located on a second side of the reference plane, the first side and the second side are opposite, and the reference plane is a plane in which the center lines of the plurality of tube parts 112 are located.

[0083] In this way, the receiving optical fiber 302 and the excitation optical fiber 301 are located on both sides of the plane in which the center lines of the tube parts 112 are located, facilitating the differentiation between the receiving optical fiber 302 and the excitation optical fiber 301, so that they do not interfere with each other, and increasing the layout reliability.

[0084] In some embodiments, the optical fiber limiting assembly 12 provides a tensioning force to the plurality of optical fibers 300, so that the ends of the plurality of optical fibers 300 are detachably abutted against the corresponding insertion holes 112b, so that the ends of the optical fibers 300 are fixed relative to the insertion holes 112b.

[0085] That is, in this embodiment, under the action of the tensioning force, the ends of the plurality of optical fibers 300 can be closely attached to the heat conduction component 11, avoiding displacement due to vibration or other external forces, and improving the transmission reliability of the excitation light and the fluorescent signal.

[0086] By fixing the end of the optical fiber 300 away from the heat conduction component 11, the influence of the end of the optical fiber 300 on the temperature of the heat conduction component 11 can be reduced, and the insertion stability of the end of the optical fiber 300 and the insertion hole 112b is high, the influence of the adhesive on the fluorescent reaction is reduced, the fluorescent reaction reliability is increased, and when the optical fiber 300 needs to be repaired and replaced, the tensioning force of the optical fiber limiting assembly 12 on the optical fiber 300 is only needed to be released, and the optical fiber 300 can be directly separated from the insertion hole 112b, the separation operation is simple and reliable, maintenance and repair and device replacement are facilitated, and the optical fiber 300 is not damaged.

[0087] In some embodiments, referring to Figures 3 to 7 , the amplification module 100 further comprises a pressing assembly 15 and at least one first connecting piece 16.

[0088] The heat conduction component 11 comprises a tube seat 111, the tube seat 111 is in heat conduction cooperation with the temperature control unit, and the plurality of tube parts 112 are connected to one side of the tube seat 111 away from the temperature control unit.

[0089] The compression assembly 15 comprises a compression plate 151 and at least one column structure 152, the compression plate 151 and the at least one column structure 152 are connected, the column structure 152 is at least partially arranged between the compression plate 151 and the tube seat 111, the first connecting member 16 connects the compression plate 151 and the bearing assembly 13, and the compression assembly 15 compresses the tube seat 111 through the column structure 152; one end of the plurality of optical fibers 300 close to the heat conduction component 11 is arranged between the compression plate 151 and the tube seat 111.

[0090] Here, the tube seat 111 is used to directly contact the temperature control unit, so as to transfer heat and / or cold from the temperature control unit to the plurality of tube portions 112, so that the plurality of tube portions 112 can obtain a consistent temperature, thereby facilitating to ensure the reaction consistency of the fluorescence reaction.

[0091] In this embodiment, the compression plate 151 and the bearing assembly 13 are connected through the first connecting member 16, so as to connect the compression assembly 15 and the bearing assembly 13, and at the same time, the column structure 152 compresses the tube seat 111, so that the position of the tube seat 111 is fixed, and the influence of vibration or other external factors is reduced, thereby facilitating to continuously obtain heat and / or cold from the temperature control unit and conduct to the heat conduction component 11, and increasing the heat conduction reliability. At the same time, the column structure 152 is arranged, so that the compression plate 151 does not directly contact the tube seat 111, that is, the contact area with the tube seat 111 is reduced while the reliable fixation of the heat conduction component 11 is realized, so that the temperature control unit has sufficient space for heat dissipation, and the working reliability of the sample analyzer is increased.

[0092] The number of the column structure 152 can be one or multiple. Exemplarily, the number of the column structure 152 is multiple, so as to increase the connection stability.

[0093] In some embodiments, referring to Figure 5 , at least part of the plurality of optical fibers 300 is arranged on one side of the one or more column structures 152 away from the tube portions 112, so that the column structure 152 assists in tensioning the optical fiber 300.

[0094] In this embodiment, the column structure 152 can assist in tensioning the optical fiber 300 while compressing the tube seat 111. When the optical fiber 300 is arranged on the column structure 152, the column structure 152 can exert a certain tension on the optical fiber 300, so as to help to maintain the appropriate tension of the optical fiber 300, reduce the situation that the part of the optical fiber 300 between the heat conduction component 11 and the optical fiber limiting assembly 12 is loosened or bent, further increase the arrangement stability of the optical fiber 300, the appropriate tension can reduce signal attenuation and avoid the loss caused by physical bending, and ensure the transmission performance of the optical fiber 300.

[0095] Of course, the optical fibers 300 wound on the column structures 152 can be arranged more neatly, and by reasonably arranging the extension paths of the optical fibers 300, the crossing probability of the optical fibers 300 can be reduced, and the layout reliability can be improved.

[0096] Meanwhile, the optical fibers 300 wound on the column structures 152 away from the tube part 112 can also reduce the influence of the optical fibers 300 on the temperature of the heat conduction component 11, and improve the fluorescence reaction reliability.

[0097] For example, referring to Figure 5 and Figure 6 , the number of column structures 152 is multiple, the multiple column structures 152 are arranged away from the tube part 112, and each tube part 112 is provided with one column structure 152 on each of the opposite sides in the second direction. In this way, while increasing the connection stability, it is also convenient to wind the multiple excitation optical fibers 301 and the multiple receiving optical fibers 302 respectively.

[0098] In some embodiments, referring to Figures 3 to 7 , the number of first connecting parts 16 is multiple, the multiple first connecting parts 16 include first parts 161 and second parts 162, the first parts 161 and the second parts 162 are located on the opposite sides of the multiple tube parts 112 in the second direction, and the ends of the multiple optical fibers 300 close to the heat conduction component 11 are located between the first parts 161 and the second parts 162.

[0099] The second direction, the first direction, and the height direction of the nucleic acid detection module are perpendicular to each other in pairs.

[0100] In this embodiment, the arrangement of the first parts 161 and the second parts 162 increases the connection stability of the pressing assembly 15 and the bearing assembly 13, makes the forces on each part of the tube seat 111 uniform, and also limits the ends of the multiple optical fibers 300 close to the heat conduction component 11 between the first parts 161 and the second parts 162, thereby positioning and limiting the positions of the optical fibers 300, and further increasing the arrangement stability and layout compactness of the optical fibers 300.

[0101] The specific structure of the optical fiber limiting assembly 12 is not limited.

[0102] In some embodiments, referring to Figures 3 to 5 , the optical fiber limiting assembly 12 includes first clamping parts 121 and second clamping parts 122, the first clamping parts 121 are arranged on the bearing assembly 13, the first clamping parts 121 and / or the second clamping parts 122 are provided with at least one wire fixing groove 12a, and the first clamping parts 121 and the second clamping parts 122 are detachably connected and clamp the multiple optical fibers 300.

[0103] Here, the wire fixing groove 12a can be provided only in the first clamping part 121, only in the second clamping part 122, or defined by the first clamping part 121 and the second clamping part 122 together, which is not limited here.

[0104] The first clamping part 121 and the second clamping part 122 are detachably connected, that is, during assembly, the first clamping part 121 and the second clamping part 122 can be first in a separated state, the optical fiber 300 is first connected with one of the first clamping part 121 and the second clamping part 122, and then the first clamping part 121 and the second clamping part 122 are connected, so as to clamp the optical fiber 300; when the optical fiber 300 needs to be repaired and replaced, the connection relationship between the first clamping part 121 and the second clamping part 122 is released, the optical fiber 300 can be taken out and separated from the heat conducting part 11, and the operation is simple and convenient.

[0105] For example, referring to Figures 6 to 8 , the wire fixing groove 12a is provided in the first clamping part 121, that is, the optical fiber 300 is first matched with the wire fixing groove 12a of the first clamping part 121 to determine the position of the optical fiber 300, and then the first clamping part 121 and the second clamping part 122 are connected, the part of the optical fiber 300 arranged in the wire fixing groove 12a is limited between the first clamping part 121 and the second clamping part 122, and the optical fiber 300 is clamped, so as to realize the tensioning of the optical fiber 300.

[0106] In this embodiment, the first clamping part 121 and the second clamping part 122 clamp the optical fiber 300, so that the position of the optical fiber 300 is fixed and reliable, and each optical fiber 300 can be firmly fixed at its position, reducing the probability of sliding and loosening. The first clamping part 121 and the second clamping part 122 are detachably connected, which can facilitate the optical fiber 300 to be first matched with one of them and then connected with the other under the condition that the optical fiber 300 naturally extends, and the appropriate clamping force is applied to make the first clamping part 121 and the second clamping part 122 clamp the optical fiber 300. By adjusting the pressure between the first clamping part 121 and the second clamping part 122, the necessary tensioning force can be provided without damaging the optical fiber 300, so as to ensure the stable contact between the end of the optical fiber 300 and the heat conducting part 11. When the optical fiber 300 needs to be disassembled, the optical fiber 300 can be easily taken out for cleaning, calibration and replacement by only releasing the connection between the first clamping part 121 and the second clamping part 122, which is convenient to disassemble and has strong clamping reliability.

[0107] The detachable connection mode of the first clamping part 121 and the second clamping part 122 is not limited, which can be screwing, clamping, etc., which is not limited here.

[0108] In some embodiments, referring to Figure 9The outer periphery of the optical fiber 300 is provided with a hard structure 400, and the first clamping portion 121 and the second clamping portion 122 clamp the hard structure 400 to fix the optical fiber 300.

[0109] Here, the hard structure 400 refers to a structure that has a certain firmness and is not easy to deform.

[0110] In this embodiment, the provision of the hard structure 400 can improve the mechanical strength of the optical fiber 300. When the first clamping portion 121 and the second clamping portion 122 clamp the optical fiber 300, the hard structure 400 can isolate the optical fiber 300 from the first clamping portion 121 and the second clamping portion 122. The hard structure 400 can protect the optical fiber 300, reduce the probability of abrasion of the optical fiber 300, reduce unnecessary pressure or deformation on the optical fiber 300, and increase the clamping reliability of the optical fiber 300.

[0111] In some embodiments, referring to Figure 9 The hard structure 400 includes a metal ring that surrounds the outer periphery of the optical fiber 300.

[0112] In this embodiment, the metal ring has a simple structure and a simple cooperation mode with the optical fiber 300, and can provide sufficient mechanical strength and protection for the optical fiber 300.

[0113] It can be understood that the size of the metal ring can be set according to the size of the optical fiber 300 and the fixing groove, so as to at least wrap the part of the optical fiber 300 located in the wire fixing groove 12a.

[0114] In some embodiments, referring to Figures 5 to 9 The groove wall of the wire fixing groove 12a has a stepped surface 12b facing the heat conduction member 11, and the hard structure 400 has an abutting surface 400a that abuts with the stepped surface 12b in the first direction.

[0115] In this embodiment, the provision of the stepped surface 12b can provide a clear stop point and mounting position. When the optical fiber 300 is installed, the abutting surface 400a of the hard structure 400 is directly cooperated with the stepped surface 12b, and then the end of the optical fiber 300 is cooperated with the heat conduction member 11, that is, the fixation of the optical fiber 300 is realized. In this way, the cooperation mode is simple. When assembling, the tight cooperation between the optical fiber 300 and the heat conduction member 11 can be realized directly according to the cooperation between the hard structure 400 and the stepped surface 12b. The cooperation between the stepped surface 12b and the abutting surface 400a can accurately position the hard structure 400 at the same position, so that the consistency of the cooperation between the end of the optical fiber 300 and the heat conduction member 11 is high, and the optical fiber 300 does not need to be cooperated with the heat conduction member 11 in advance. The installation efficiency and reliability are high.

[0116] In some embodiments, referring to Figure 5 and Figure 6 the number of the fixed wire slots 12a is multiple, and the multiple fixed wire slots 12a are arranged along the second direction.

[0117] Here, the multiple fixed wire slots 12a arranged along the second direction facilitate fixing the excitation optical fiber 301 and the receiving optical fiber 302 respectively, and increase the fixing reliability of the optical fiber 300, while making the fixing positions of the multiple optical fibers 300 along the first direction on the optical fiber limiting assembly 12 consistent.

[0118] In some embodiments, one optical fiber 300 is accommodated in one fixed wire slot 12a. In this way, each fixed wire slot 12a only accommodates one optical fiber 300, so that each optical fiber 300 can obtain a more consistent tensioning force, accurate fixing and positioning, and high fixing reliability.

[0119] In other embodiments, referring to Figure 5 one optical fiber 300 is accommodated in one fixed wire slot 12a, and the stepped surface 12b extends along the height direction of the nucleic acid detection module, the multiple optical fibers 300 accommodated in the same fixed wire slot 12a are stacked along the height direction of the nucleic acid detection module, and each of the multiple optical fibers 300 abuts against the stepped surface 12b.

[0120] In this embodiment, one fixed wire slot 12a accommodates multiple optical fibers 300, so that the number of fixed wire slots 12a can be reduced, the occupied size of the optical fibers 300 can be reduced, and the wiring complexity of the optical fibers 300 in the second direction can be reduced. By stacking the optical fibers 300, more optical fibers 300 can be accommodated in a limited space, and the stepped surface 12b extends along the height direction, i.e., can facilitate abutting against each optical fiber 300, for example, abutting against the abutting surface 400a of the hard structure 400 provided on each optical fiber 300, i.e., providing a fixed mounting point, and increasing the mounting reliability.

[0121] The specific structure of the first clamping part 121 is not limited.

[0122] In some embodiments, referring to Figure 6 and Figure 8 the first clamping part 121 includes a first connecting sub-part 1211, a second connecting sub-part 1212, and a third connecting sub-part 1213 arranged at intervals along the second direction, the interval region between the first connecting sub-part 1211 and the second connecting sub-part 1212 forms a first groove 121a, and the interval region between the second connecting sub-part 1212 and the third connecting sub-part 1213 forms a second groove 121b.

[0123] The first clamping part 121 further comprises a plurality of partition walls 1214, some of the partition walls are arranged in the first groove 121a to divide the first groove 121a into a plurality of wire fixing grooves 12a, and the other partition walls 1214 are arranged in the second groove 121b to divide the second groove 121b into a plurality of wire fixing grooves 12a.

[0124] The plurality of excitation optical fibers 301 pass through the plurality of wire fixing grooves 12a of the first groove 121a, and the plurality of receiving optical fibers 302 pass through the plurality of wire fixing grooves 12a of the second groove 121b.

[0125] Here, the area for fixing the excitation optical fibers 301 is defined by the first groove 121a, and the area for fixing the receiving optical fibers 302 is defined by the second groove 121b, so that the installation positions of the excitation optical fibers 301 and the receiving optical fibers 302 can be distinguished, and the probability of cross interference caused by disorderly arrangement of the excitation optical fibers 301 and the receiving optical fibers 302 is reduced.

[0126] Some of the partition walls 1214 divide the first groove 121a into a plurality of wire fixing grooves 12a, that is, the plurality of excitation optical fibers 301 can be fixed separately to increase the fixing reliability, and the other partition walls 1214 divide the second groove 121b into a plurality of wire fixing grooves 12a, which can facilitate the separate fixing of the plurality of receiving optical fibers 302 to increase the fixing reliability.

[0127] The optical fiber limiting assembly 12 comprises a plurality of second connecting members 123, and the second clamping part 122 is connected to the first connecting sub-part, the second connecting sub-part and the third connecting sub-part through the plurality of second connecting members 123.

[0128] Here, the second clamping part 122 is connected to the first connecting sub-part 1211, the second connecting sub-part 1212 and the third connecting sub-part 1213 through the second connecting members 123, that is, the clamping force between the second clamping part 122 and the first connecting sub-part 1211, the second connecting sub-part 1212 and the third connecting sub-part 1213 is relatively uniform, thereby facilitating the provision of relatively uniform clamping force for each excitation optical fiber 301 and receiving optical fiber 302, and reducing the situation of uneven stress.

[0129] The specific form of the second connecting member 123 is not limited, and exemplarily, the second connecting member 123 can be a screw.

[0130] In some embodiments, referring to Figures 2 to 7 The carrying assembly 13 comprises a bottom plate 131 and a support structure 132 arranged on the bottom plate 131, and the expansion module 100 is arranged on the support structure 132, wherein at least a part of the support structure 132 is configured as a heat dissipation structure, and the heat dissipation structure is used for heat dissipation of the temperature control unit.

[0131] The bottom plate 131 is configured to provide a mounting platform for supporting the support structure 132 and the heat conduction component 11 and the temperature control unit disposed on the support structure 132, and the support structure 132 is configured to connect the heat conduction component 11 and the temperature control unit together, so that the temperature control unit provides heat and / or cold to the heat conduction component 11.

[0132] Exemplarily, the optical fiber limiting assembly 12 is disposed on the bottom plate 131 and located at one side of the support structure 132 in the first direction, facilitating limiting the optical fiber 300 extending in the first direction and increasing the layout reliability, and meanwhile, the optical fiber limiting assembly 12 is disposed outside the support structure 132, i.e., can avoid the temperature control unit and the heat conduction component 11, and will not interfere with the operation of the temperature control unit and the heat conduction component 11.

[0133] It can be understood that the form of the heat dissipation structure is not limited, and exemplarily, can be a heat dissipation fin.

[0134] It should be noted that in other embodiments, the support structure 132 can also not have a heat dissipation function, i.e., a heat dissipation component can be disposed outside the support structure 132 to dissipate heat for the temperature control unit, and the heat dissipation component can be a heat dissipation pipe or a heat dissipation fin, which is not limited here.

[0135] It can be understood that in other embodiments, the amplification module 100 can also not be provided with the bottom plate 131, but directly provide support for the temperature control unit and the heat conduction component 11 through the support structure 132, and the optical fiber limiting assembly 12 is connected to the support structure 132 in the first direction.

[0136] Please refer to Figure 2 and Figure 3 The nucleic acid detection module includes a cover 14, the bottom side of the cover 14 is formed with an opening 14a, and the cover 14 is provided with two openings 14b at opposite ends in the first direction, the cover 14 is arranged outside the outer periphery of the amplification module 100 and the support structure 132, the opening 14a of the cover 14 abuts against the bottom plate 131, and one of the openings 14b is used for allowing the plurality of optical fibers 300 to pass through; and the other opening 14b is used for at least allowing the cable 500 to pass through, and the cable 500 is used for at least electrically connecting with the temperature control unit.

[0137] In this embodiment, the hollow chamber formed by the cover 14 and the bottom plate 131 accommodates the heat conduction component 11, the temperature control unit and the support structure 132, i.e., can help to maintain the stability of the internal environment, and facilitate maintaining stable temperature conditions without being disturbed by external factors. Exemplarily, the cover 14 can be made of a heat preservation material.

[0138] Exemplarily, the hollow chamber can be a sealed chamber, i.e., the optical fiber 300 can pass out of one of the openings 14b without damaging the sealing, and the cable 500 can pass out of the other opening 14b without damaging the sealing.

[0139] The cable 500 can obtain electric energy from the outer cover 14 and transmit to the temperature control unit to enable the temperature control unit to work stably. The outer cover 14 is provided with openings 14b at both ends in the first direction to facilitate the cable 500 and the optical fiber 300 to not interfere with each other, thereby increasing the layout reliability.

[0140] The sample analyzer provided in the embodiments of the present application can be used in the medical field.

[0141] It can be understood that the sample analyzer is a device for automatically or semi-automatically analyzing various biological, chemical or physical samples. The sample analyzer can be applied in the medical field, scientific research, environmental monitoring, food and pharmaceutical fields, etc. to detect and measure specific components or characteristics in the sample. In the embodiments of the present application, the sample analyzer is taken as an example for description in the medical field.

[0142] The sample analyzer can automatically complete the processing, mixing, reaction, cleaning, detection and other steps of the sample, reduce manual operation, improve efficiency and reduce human errors.

[0143] The sample analyzer can be a polymerase chain reaction (PCR) instrument, a clinical chemistry analyzer, an immune analyzer, a blood cell analyzer, a urine analyzer, a gene sequencer, etc.

[0144] Exemplarily, the sample analyzer is taken as a polymerase chain reaction (PCR) instrument for description in the embodiments of the present application.

[0145] The sample analyzer comprises a nucleic acid detection module, a light emitting module, a light receiving module and a control module.

[0146] It can be understood that the nucleic acid detection module is the nucleic acid detection module in any of the embodiments of the present application.

[0147] The light emitting module is configured to provide excitation light through the excitation optical fiber 301.

[0148] The light receiving module is configured to receive the optical signal transmitted by the receiving optical fiber 302 and convert the received optical signal into an electric signal.

[0149] The control module is configured to obtain the electric signal and obtain a sample detection result according to the electric signal.

[0150] Exemplarily, the nucleic acid detection module can extract nucleic acid from a liquid containing a sample by using a nucleic acid extraction device to obtain a nucleic acid extraction liquid.

[0151] The nucleic acid extraction device can be an apparatus for separating and purifying DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) from a sample. The sample can be blood, tissue, cells, saliva, etc., and the sample is illustratively saliva. The main purpose of nucleic acid extraction is to remove proteins, lipids and other impurities to obtain a high-purity nucleic acid extract.

[0152] The amplification module 100 is used to amplify the nucleic acid extract in the amplification container to obtain the test liquid; the light emitting module provides excitation light through the excitation optical fiber 301, the excitation optical fiber 301 guides the excitation light to the test liquid in the amplification container in the accommodation cavity 112a, so that the test liquid is excited to generate a fluorescence signal; the receiving optical fiber 302 guides the fluorescence signal to the light receiving module, the light receiving module converts the received fluorescence signal into an electrical signal, and the control module obtains the sample detection result according to the electrical signal.

[0153] The light emitting module is used to generate excitation light of a specific wavelength and irradiate the test liquid through the excitation optical fiber 301.

[0154] Illustratively, the light emitting module can include a light source, an optical element such as a filter, etc. The light source emits light of a specific wavelength, and only excitation light that meets the requirements passes through the filter after being filtered. The lens can focus and transmit the excitation optical fiber 301 to the amplification container, so that the test liquid is excited to generate a fluorescence signal.

[0155] Illustratively, the light receiving module can include a photodetector, a signal amplification circuit, etc. The photodetector converts the fluorescence signal into an electrical signal, and the signal amplification circuit amplifies the weak electrical signal for subsequent signal processing.

[0156] The control module can process and analyze the received electrical signal, calculate the fluorescence intensity or other related parameters, and thus obtain the detection result of the sample.

[0157] In some embodiments, the sample analyzer includes a carrying table 200, and the number of nucleic acid detection modules is multiple. The multiple nucleic acid detection modules are arranged on the carrying table 200 and are arranged at intervals along a second direction.

[0158] In this embodiment, the carrying table 200 can provide installation support for the nucleic acid detection modules and integrate multiple nucleic acid detection modules together to increase installation stability. The multiple nucleic acid detection modules are arranged at intervals along the second direction, that is, the arrangement of the multiple nucleic acid detection modules can be performed without increasing the size of the sample analyzer along the first direction. When the optical fiber 300 extends substantially along the first direction, the overall arrangement size of the nucleic acid detection modules along the second direction can be small, so that more nucleic acid detection modules can be arranged without increasing the overall size of the sample analyzer, so as to process more types and amounts of samples and improve detection efficiency.

[0159] In some embodiments, the plurality of nucleic acid detection modules are arranged in a matrix. That is, the plurality of nucleic acid detection modules are arranged in a two-dimensional or multi-dimensional array, which can be arranged in rows and columns to form a rectangular or square grid, each nucleic acid detection module having an independent temperature control unit and heat conduction component 11. The matrix arrangement can improve the processing capacity and flexibility of the sample analyzer, while increasing the reliability of the layout.

[0160] In some embodiments, the carrier table 200 is a one-piece structure.

[0161] In this embodiment, the carrier table 200 is manufactured in one piece, which is high in production efficiency and low in manufacturing difficulty, and is convenient for assembly.

[0162] It can be understood that the carrier table 200, as a force receiving component, can have a certain structural strength. For example, the carrier table 200 can be a one-piece metal piece. Of course, in other embodiments, the carrier table 200 can also be a one-piece plastic piece, as long as it has sufficient structural strength.

[0163] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms is not necessarily for the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the skilled in the art can combine the different embodiments or examples described in the present application and the features of the different embodiments or examples without contradiction.

[0164] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. For those skilled in the art, the present application can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A nucleic acid detection module, characterized by, The application relates to a nucleic acid detection module, comprising: a bearing assembly; an amplification module arranged on the bearing assembly, the amplification module comprising a temperature control unit and a heat conducting component, the temperature control unit being used for providing heat and / or cold to the heat conducting component, the heat conducting component comprising a plurality of pipe sections arranged along a first direction, the pipe sections having accommodating cavities for accommodating amplification containers loaded with samples, pipe walls of the pipe sections being provided with at least two insertion holes; a plurality of optical fibers, the plurality of optical fibers comprising a plurality of excitation optical fibers and a plurality of receiving optical fibers, one of the insertion holes of the same pipe section being used for inserting an end of the excitation optical fiber, and the other insertion hole being used for inserting an end of the receiving optical fiber; an optical fiber limiting assembly arranged on the bearing assembly and located on a first side of the heat conducting component in the first direction, the optical fiber limiting assembly being provided with at least one wire fixing groove, the plurality of optical fibers extending along a direction away from the pipe sections and passing through the at least one wire fixing groove on the first side of the amplification module in the first direction.

2. The nucleic acid detection module of claim 1, wherein, The extension direction of the wire fixing groove is parallel to the first direction.

3. The nucleic acid detection module of claim 1, wherein, The plurality of excitation optical fibers are located on a first side of a reference plane, the plurality of receiving optical fibers are located on a second side of the reference plane, the first side and the second side are opposite, and the reference plane is a plane in which center lines of the plurality of pipe sections are located.

4. The nucleic acid detection module of claim 1, wherein, The optical fiber limiting assembly provides a tensioning force to the plurality of optical fibers, so that the ends of the plurality of optical fibers are detachably abutted with the corresponding insertion holes, and the ends of the optical fibers are fixed relative to the insertion holes.

5. The nucleic acid detection module of claim 1, wherein, The amplification module further comprises a pressing assembly and at least one first connecting piece; The heat conducting component comprises a pipe seat, the pipe seat is in heat conduction cooperation with the temperature control unit, and the plurality of pipe sections are connected to a side of the pipe seat away from the temperature control unit; The pressing assembly comprises a pressing plate and at least one column structure, the pressing plate and the at least one column structure are connected, the column structure is at least partially arranged between the pressing plate and the pipe seat, the first connecting piece connects the pressing plate and the bearing assembly, the pressing assembly presses the pipe seat through the column structure, and one end of the plurality of optical fibers close to the heat conducting component is arranged between the pressing plate and the pipe seat.

6. The nucleic acid detection module of claim 5, wherein, At least part of the plurality of optical fibers is arranged on a side of one or more column structures away from the pipe sections, so that the column structures assist in tensioning the optical fibers.

7. The nucleic acid detection module of claim 5, wherein, The number of the first connecting pieces is plural, the plurality of first connecting pieces comprise a first part and a second part, the first part and the second part are located on opposite sides of the plurality of pipe sections in a second direction, and one end of the plurality of optical fibers close to the heat conducting component is located between the first part and the second part. The second direction, the first direction and a height direction of the nucleic acid detection module are perpendicular to each other.

8. The nucleic acid detection module of claim 1, wherein, The optical fiber limiting assembly comprises a first clamping part and a second clamping part, the first clamping part is arranged on the bearing assembly, the first clamping part and / or the second clamping part are provided with the at least one wire fixing groove, the first clamping part and the second clamping part are detachably connected and clamp the plurality of optical fibers.

9. The nucleic acid detection module of claim 8, wherein, The outer periphery of the optical fiber is provided with a hard structure, and the first clamping part and the second clamping part clamp the hard structure to achieve fixation of the optical fiber.

10. The nucleic acid detection module of claim 9, wherein, The hard structure comprises a metal ring which surrounds the outer periphery of the optical fiber.

11. The nucleic acid detection module of claim 9, wherein, The groove wall of the wire fixing groove has a stepped surface facing the heat conducting part, and the hard structure has an abutting surface which abuts with the stepped surface in the first direction.

12. The nucleic acid detection module of claim 11, wherein, The number of the wire fixing grooves is multiple, and the multiple wire fixing grooves are arranged along a second direction. A single wire fixing groove accommodates one optical fiber, or a single wire fixing groove accommodates multiple optical fibers, the stepped surface extends along the height direction of the nucleic acid detection module, the multiple optical fibers accommodated in the same wire fixing groove are stacked along the height direction of the nucleic acid detection module and abut with the stepped surface. The second direction, the first direction and the height direction of the nucleic acid detection module are perpendicular to each other.

13. The nucleic acid detection module of claim 12, wherein, The first clamping part comprises a first connecting sub-part, a second connecting sub-part and a third connecting sub-part which are arranged at intervals in the second direction, a spacing region between the first connecting sub-part and the second connecting sub-part forms a first groove, and a spacing region between the second connecting sub-part and the third connecting sub-part forms a second groove. The first clamping part further comprises multiple spacing walls, part of the spacing walls are arranged in the first groove to separate the first groove into multiple wire fixing grooves, and another part of the spacing walls are arranged in the second groove to separate the second groove into multiple wire fixing grooves. The multiple excitation optical fibers pass through the multiple wire fixing grooves of the first groove, and the multiple receiving optical fibers pass through the multiple wire fixing grooves of the second groove. The optical fiber limiting assembly comprises multiple second connecting members, and the second clamping part is connected with the first connecting sub-part, the second connecting sub-part and the third connecting sub-part through the multiple second connecting members.

14. The nucleic acid detection module of claim 1, wherein, The bearing assembly comprises a bottom plate and a support structure arranged on the bottom plate, and the amplification module is arranged on the support structure, wherein at least part of the support structure is configured as a heat dissipation structure for dissipating heat for the temperature control unit. The nucleic acid detection module comprises an outer cover, an open end is formed on the bottom side of the outer cover, and two openings are arranged at opposite ends of the outer cover in the first direction, the outer cover covers the outer periphery of the amplification module and the support structure, and the open end of the outer cover abuts with the bottom plate, one of the openings is used for passing the multiple optical fibers, and the other of the openings is used for passing at least a cable which is used for electrical connection with the temperature control unit.

15. A sample analyzer characterized by, The nucleic acid detection module comprises an outer cover, an open end is formed on the bottom side of the outer cover, and two openings are arranged at opposite ends of the outer cover in the first direction, the outer cover covers the outer periphery of the amplification module and the support structure, and the open end of the outer cover abuts with the bottom plate, one of the openings is used for passing the multiple optical fibers, and the other of the openings is used for passing at least a cable which is used for electrical connection with the temperature control unit. The nucleic acid detection module comprises an outer cover, an open end is formed on the bottom side of the outer cover, and two openings are arranged at opposite ends of the outer cover in the first direction, the outer cover covers the outer periphery of the amplification module and the support structure, and the open end of the outer cover abuts with the bottom plate, one of the openings is used for passing the multiple optical fibers, and the other of the openings is used for passing at least a cable which is used for electrical connection with the temperature control unit. The nucleic acid detection module comprises an outer cover, an open end is formed on the bottom side of the outer cover, and two openings are arranged at opposite ends of the outer cover in the first direction, the outer cover covers the outer periphery of the amplification module and the support structure, and the open end of the outer cover abuts with the bottom plate, one of the openings is used for passing the multiple optical fibers, and the other of the openings is used for passing at least a cable which is used for electrical connection with the temperature control unit. The nucleic acid detection module comprises an outer cover, an open end is formed on the bottom side of the outer cover, and two openings are arranged at opposite ends of the outer cover in the first direction, the outer cover covers the outer periphery of the amplification module and the support structure, and the open end of the outer cover abuts with the bottom plate, one of the openings is used for passing the multiple optical fibers, and the other of the openings is used for passing at least a cable which is used for electrical connection with the temperature control unit. ​ 16. The sample analyzer of claim 15, wherein, The sample analyzer comprises a bearing table, the number of the nucleic acid detection modules is multiple, and the multiple nucleic acid detection modules are arranged on the bearing table and are arranged at intervals along a second direction. The second direction intersects the first direction and is perpendicular to a height direction of the sample analyzer.

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