Flow type refrigeration multifunctional storage experiment kit

Abstract

The utility model discloses a flow type refrigeration multifunctional storage experiment kit, and relates to the field of flow cytometry, in particular to a flow type refrigeration multifunctional storage experiment kit which comprises a kit body with an opening in the top and an opening in one side, an experiment box used for placing test tubes and a pull type storage box used for storing cooling media. A second groove is formed in the middle of the body, a third groove is formed in the lower middle portion of the body, a first clamping groove is formed in the pull-out type storage box, and a third clamping groove is formed in the experiment box. When a low-temperature test needs to be carried out, the first clamping groove in the drawing type storage box is pushed into the body along the second groove, and the problems that due to the fact that an existing refrigerator is repeatedly opened and closed, the internal temperature of the refrigerator is unstable, the pollution risk is increased, the fluorescence intensity is attenuated and quenched possibly when a reagent is placed at the room temperature for a long time, and the test time is shortened are solved. And the experiment effect is influenced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of flow cytometry, concretely to a flow cytometry refrigeration multifunctional storage experiment kit. BACKGROUND

[0002] Flow cytometry is a biological technique for counting and sorting microscopic particles suspended in a stream of fluid. The technique can be used to analyze a succession of individual cells flowing past an optical or electronic detector for a variety of parameters. Flow cytometry is a technology that allows for rapid, one-by-one analysis and sorting of single cells or other biological particles in suspension by detecting labeled fluorescent signals. Its characteristics are that it can quantitatively measure cell DNA content, cell volume, protein content, enzyme activity, cell membrane receptors and surface antigens and many other important parameters by rapidly measuring Coulter resistance, fluorescence, light scattering and light absorption. According to these parameters, cells of different properties are separated to obtain pure cell populations for biological and medical research.

[0003] The conventional flow cytometry is generally operated at room temperature in the fluorescent labeling step, and the reagent is repeatedly exposed to room temperature for a long time, which may cause fluorescence intensity attenuation quenching and affect the experimental effect. When the fluorescent reagent reaction system needs to be incubated in a low-temperature environment, it is generally necessary to independently place the experimental refrigerator or reagent refrigerator, which is complicated and has a high possibility of contamination.

[0004] When the reaction environment needs to be incubated at low temperature in the flow experiment process, the reaction system is usually placed in a 2-8 DEG C medical refrigerator; in addition, the fluorescent reagent of flow cytometry also needs to be stored in a 2-8 DEG C medical refrigerator, and each use needs to be moved from the medical refrigerator to room temperature, and then placed in the medical refrigerator again after the experiment is completed. The repeated opening and closing of the refrigerator may cause the temperature inside the refrigerator to be unstable, the risk of contamination to increase, and the fluorescence intensity to attenuate and quench for a long time, which affects the experimental effect.

[0005] Therefore, a novel flow cytometry refrigeration multifunctional storage experiment kit needs to be designed to solve the above problems. UTILITY MODEL CONTENT

[0006] The utility model aims at providing a flow cytometry refrigeration multifunctional storage experiment kit to solve the problems in the background art.

[0007] In order to achieve the above object, the utility model provides the following technical scheme: a flow type refrigeration multifunctional storage experimental kit, including the reagent box body of top and one side opening, the experimental box for placing test tube and the pull-out type storage box for storing cooling medium, the middle part of the body is equipped with groove two, the middle lower part of the body is equipped with groove three, the pull-out type storage box is equipped with the card slot one, the experimental box is equipped with the card slot three, when needing to carry out low temperature test, the card slot one on the pull-out type storage box is pushed into the body inside along the groove two, when needing to carry out short term low temperature storage, the card slot one on the pull-out type storage box is pushed into the body inside along the groove three.

[0008] Preferably, the experimental box includes a box body, the top of the box body is provided with a multi-well plate one for placing test tubes, and the middle of the box body is provided with a multi-well plate two matched with the multi-well plate one.

[0009] Preferably, the body is provided with a pull-tab type plate, the upper part of the body is provided with a groove one, and the pull-tab type plate is provided with a card slot two matched with the groove one.

[0010] Preferably, the pull-out type storage box and the experimental box are respectively provided with handles.

[0011] Preferably, the pull-tab type plate is provided with a recess.

[0012] Compared with the prior art, the utility model has the beneficial effects that:

[0013] 1. The utility model adopts the overall box type structure, and is divided into upper and lower parts, the upper part has the pullable experimental box and the pull-tab type plate, the lower part is the pull-out type storage box, combination is convenient, can create the low temperature environment of temporary operation for some flow type low temperature experiments, and moreover, the structure is simple, the applicable range is wide, the upper pullable box type can be used for flow type tube experimental operation, the pull-tab type plate can be used for temporary low temperature placement, the integrated design can make the appearance simple and decent, safe and stable.

[0014] 2. In the utility model, the position of the pull-out type storage box can be switched, the requirement for refrigerator classification storage is reduced, ice blocks can be placed in the pull-out type storage box, a short term low temperature environment can be provided for the mixture or reagent in the upper test tube, operation is simple, the possibility of pollution in the experimental process is avoided, the flow type fluorescent antibody is ensured to be in a low temperature storage state in the experimental process, work efficiency is improved, and detection quality is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is the overall structure schematic view of the utility model.

[0016] Figure 2 It is the front view of the utility model.

[0017] Figure 3 For the utility model Figure 2 The sectional view of A-A.

[0018] Figure 4 For the utility model Figure 3 The enlarged view of A.

[0019] Figure 5 For the utility model Figure 3 The enlarged view of B.

[0020] Figure 6 The schematic diagram of the pull-out piece type flat plate structure of the utility model.

[0021] Figure 7 The explosion view of the utility model.

[0022] In the figure: 1, the main body; 3, the pull-out type storage box; 4, recess two; 5, recess three; 6, card slot one; 7, card slot three; 8, box body; 9, multi-hole flat plate one; 10, multi-hole flat plate two; 11, pull-out piece type flat plate; 12, recess one; 13, card slot two; 14, handle; 15, recess. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] All the structures in the application are made of materials meeting the requirements of low-temperature tests.

[0025] Embodiment one

[0026] Please refer to Figures 1-7 The utility model provides a flow type refrigeration multifunctional storage experimental kit, including the reagent box body 1 of top and one side opening, the experimental box for placing test tube and the pull-out type storage box 3 for storing cooling medium, the middle part of the body 1 is equipped with recess two 4, the middle lower part of the body 1 is equipped with recess three 5, the pull-out type storage box 3 is equipped with card slot one 6, and the experimental box is equipped with card slot three 7;When needing to carry out low-temperature test, the card slot one 6 on the pull-out type storage box 3 is pushed into the inside of the body 1 along recess two 4;When needing to carry out short-term low-temperature storage, the card slot one 6 on the pull-out type storage box 3 is pushed into the inside of the body 1 along recess three 5.

[0027] The body 1 is made of heat insulation material, the top and one side of the body 1 are open, the experimental box and the pull-out storage box 3 are made of low-temperature-resistant polymer material, some test tubes can be placed on the experimental box, the test tubes are flow tubes commonly used in cold storage experiments, some cooling medium can be stored in the pull-out storage box 3, the cooling medium is one of ice bags and ice blocks, the groove two 4 is arranged on the upper middle part of the body 1, the groove three 5 is arranged on the lower middle part of the body 1, the clamping groove one 6 is fixedly installed on the two sides of the pull-out storage box 3, the clamping groove three 7 is fixedly installed on the two sides of the experimental box, the experimental box can be pulled out, and the pull-out storage box 3 can be inserted into the groove two 4 or the groove three 5 as required, so that low-temperature experiments and short-term low-temperature storage of test tubes can be conveniently performed.

[0028] Specifically, the experimental box comprises a box body 8, a multi-well plate one 9 for placing test tubes is arranged on the top of the box body 8, a multi-well plate two 10 matched with the multi-well plate one 9 is arranged on the middle part of the box body 8, the bottom of the box body 8 is made of heat-conducting material, the multi-well plate one 9 and the multi-well plate two 10 are clamped on the box body 8, the multi-well plate one 9 and the multi-well plate two 10 can conveniently place flow tubes, and the ice blocks in the pull-out storage box 3 can absorb heat on the box body 8, so that a low-temperature environment is maintained in the experimental box.

[0029] Specifically, the experimental box comprises a box body 8, a multi-well plate one 9 for placing test tubes is arranged on the top of the box body 8, a multi-well plate two 10 matched with the multi-well plate one 9 is arranged on the middle part of the box body 8, the bottom of the box body 8 is made of heat-conducting material, the multi-well plate one 9 and the multi-well plate two 10 are clamped on the box body 8, the multi-well plate one 9 and the multi-well plate two 10 can conveniently place flow tubes, and the ice blocks in the pull-out storage box 3 can absorb heat on the box body 8, so that a low-temperature environment is maintained in the experimental box.

[0030] On the basis of the above embodiment, specifically, handles 14 are arranged on the pull-out storage box 3 and the experimental box respectively, the handles 14 are located on one side of the pull-out storage box 3 and the experimental box respectively, so that the pull-out storage box 3 and the experimental box can be conveniently pulled out, and the position of the pull-out storage box 3 can be conveniently switched.

[0031] Working principle: when the low temperature test is carried out, the ice cubes are put into the pull-out storage box 3, then the pull-out storage box 3 is pushed into the groove three 5 at the bottom of the body 1, the pull-out sheet flat plate 11 is removed, then the experimental box is pushed into the groove two 4, the pull-out storage box 3 can cool the flow tube in the box body 8, the flow tube is put on the experimental box along the porous flat plate one 9 and the porous flat plate two 10, when the temperature reaches the experimental requirement, the low temperature test can be carried out;

[0032] When the short-term low temperature storage is needed, the pull-out storage box 3 at the lower part of the body 1 is pulled out first, the required ice cubes are placed, the pull-out storage box 3 is pushed into the groove two 4, then the pull-out sheet flat plate 11 is inserted into the groove one 12 at the upper part of the body 1, the short-term low temperature storage requirement can be met.

[0033] The contents not described in detail in the description belong to the prior art known by the person skilled in the art.

[0034] Although the utility model is described in detail with reference to the foregoing embodiments, the skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. A flow cytometry-based multifunctional storage experimental kit, characterized in that, The kit includes a reagent body (1) with an opening at the top and on one side, an experimental box for placing test tubes, and a pull-out storage box (3) for storing cooling medium. The body (1) has a second groove (4) in the middle, a third groove (5) in the lower middle part of the body (1), a slot (6) on the pull-out storage box (3), and a slot (7) on the experimental box. When a low-temperature test is required, the slot 1 (6) on the pull-out storage box (3) is pushed into the body (1) along the groove 2 (4); When short-term low-temperature storage is required, the slot 1 (6) on the pull-out storage box (3) is pushed into the body (1) along the groove 3 (5).

2. The multifunctional storage test kit for flow cytometry refrigeration according to claim 1, characterized in that: The experimental box includes a box body (8), the top of the box body (8) is provided with a porous plate one (9) for placing test tubes, and the middle part of the box body (8) is provided with a porous plate two (10) that cooperates with the porous plate one (9).

3. The multifunctional storage test kit for flow cytometry refrigeration according to claim 2, characterized in that: The main body (1) is provided with a pull-out plate (11), and the upper part of the main body (1) is provided with a groove (12). The pull-out plate (11) is provided with a slot (13) that cooperates with the groove (12).

4. The multifunctional storage test kit for flow cytometry refrigeration according to claim 3, characterized in that: The pull-out storage box (3) and the experimental box are respectively equipped with handles (14).

5. The multifunctional storage test kit for flow cytometry refrigeration according to claim 4, characterized in that: The pull-out flat plate (11) has a recess (15).

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