Electro-transfection device for eukaryotic cells
By employing a threaded self-locking sealing structure in the eukaryotic cell electroporation device, the problem of high dependence on air pressure in the prior art has been solved, achieving a convenient and high-precision sealing effect and simplifying the operation process.
Patent Information
- Application Number
- CN202422564099.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-23
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2034-10-23
AI Technical Summary
Existing eukaryotic cell electroporation devices require high air pressure and continuous air pressure control during the pressurization and sealing process, which leads to inconvenience in operation and insufficient accuracy.
The sealing structure adopts a threaded self-locking connection. The position of the sealing cover in the hollow cylindrical wall is adjusted by rotating the screw. The sealing is achieved by the friction of the threads, and the position of the sealing cover is fixed by the thread self-locking to avoid continuous pressure.
It achieves safe and convenient sealing operation, improves adjustment accuracy, reduces dependence on air pressure, and simplifies the operation process.
Smart Images
Figure CN223674654U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of electrotransfection, more particularly to a eukaryotic cell electrotransfection device. BACKGROUND
[0002] Eukaryotic cell electrotransfection, also known as electroporation, is a technique that uses physical means to introduce foreign molecules such as DNA, RNA, etc. into eukaryotic cells. By sending a pulse current through the electrode, a hole is punched in the cell membrane, allowing the foreign molecule to penetrate the cell membrane and enter the cell interior, thereby changing the characteristics of the cell or achieving the purpose of modifying the cell. When the electric pulse ends, the hole in the cell membrane will close quickly, and at this time the foreign molecule has successfully entered the cell interior. To avoid bubbles generated during the electrotransfection process from adhering to the electrode, a structure similar to a plug can be used to reduce the volume of the container by air pressure, thereby increasing the pressure. The plug and the inner wall of the container are usually deformed by being squeezed by the inner wall of the container, thereby increasing the adhesion of the plug to the inner wall of the container, thereby sealing the container with the plug. The normal pressure between the plug and the inner wall of the container is large, so the static friction is large, and a large air pressure is needed to push the plug. On the other hand, maintaining the position of the plug requires a continuous supply of air pressure, which requires a higher control of the air pressure, so other ways of pressurization can also be tried. SUMMARY
[0003] The utility model provides a eukaryotic cell electrotransfection device, the purpose is to keep the position of the plug through the self locking of screw thread.
[0004] The above-mentioned purpose is realized by the following technical solutions:
[0005] A eukaryotic cell electrotransfection device, comprising a base, a screw rod seat is fixedly connected to the base, a hollow cylindrical wall body is arranged on the right side of the screw rod seat, an exchange port is arranged on the right side of the upper part of the hollow cylindrical wall body, a first cover is arranged at the right end of the hollow cylindrical wall body, a second cover is arranged on the hollow cylindrical wall body to close the exchange port, a sealing cover is arranged on the left side of the inside of the hollow cylindrical wall body, a screw rod is fixedly connected to the left end of the sealing cover, and the screw rod is connected to the screw rod seat through thread cooperation.
[0006] The inner wall of the hollow cylindrical wall body is provided with two electrodes located on the right side of the sealing cover.
[0007] The upper end of the base is provided with two sockets in front and back, the lower end of the hollow cylindrical wall body is fixedly connected with two supporting pieces, and the two supporting pieces are respectively inserted into the two sockets.
[0008] The sealing cover comprises a first gland, a second gland is detachably connected to the left end of the first gland, an annular groove is formed between the first gland and the second gland, a sealing ring is arranged in the annular groove, the first gland and the second gland are respectively pressed on the left end and the right end of the sealing ring, and the outer circle of the sealing ring is attached to the inner wall of the hollow cylindrical wall body.
[0009] A bolt is arranged through the first gland, the head of the bolt is abutted against the left end of the second gland, and the tail of the bolt is connected to the first gland through thread cooperation.
[0010] An annular groove is arranged on the outer circumferential surface of the sealing ring.
[0011] The outer circumferential edge of the left end of the first gland and the outer circumferential edge of the right end of the second gland are both provided with chamfered surfaces.
[0012] The first cover is inserted into the right part of the inner wall of the hollow cylindrical wall body, the left end of the first cover is provided with an inclined surface, and the inclined surface gradually rises from left to right.
[0013] The top of the inclined surface is located on the left side of the right end edge of the exchange port.
[0014] The left end of the screw rod is provided with a prismatic groove, and a handle is inserted into the prismatic groove.
[0015] The eukaryotic cell electroporation device has the following beneficial effects:
[0016] The position of the sealing cover on the axis of the hollow cylindrical wall body is adjusted by the feeding of the screw thread connection of the screw rod, compared with the air bag mode, the sealing cover does not need a large air pressure, is safe and convenient to use, and has high adjustment precision; the position of the sealing cover relative to the hollow cylindrical wall body is fixed by the self-locking property of the screw thread, and pressure does not need to be continuously provided. BRIEF DESCRIPTION OF DRAWINGS
[0017] Figure 1 It is a cross-sectional view of the eukaryotic cell electroporation device;
[0018] Figure 2 It is a schematic view of the support piece inserted into the socket;
[0019] Figure 3 It is a schematic view of the electrode in the hollow cylindrical wall body;
[0020] Figure 4 It is a top view of the hollow cylindrical wall body;
[0021] Figure 5 It is a schematic view of the first gland and the sealing ring;
[0022] Figure 6 It is Figure 1 It is an enlarged view of the left lower side;
[0023] Figure 7 is a schematic view of the second gland structure;
[0024] Figure 8 is a schematic view of the second gland and screw;
[0025] Figure 9 is a schematic view of the first cover and bevel;
[0026] Figure 10 is a schematic view of the Figure 1 is an enlarged view of the upper right corner.
[0027] In the figure: base 1-1; screw seat 1-2; hollow cylindrical wall 2-1; exchange port 2-2; electrode 2-3; support 2-4; first gland 3-1; second gland 3-2; sealing ring 3-3; bolt 3-4; screw 3-5; first cover 4-1; bevel 4-2; second cover 4-3. DETAILED DESCRIPTION
[0028] An electric transfection device for eukaryotic cells, with reference to Figures 1 to 4 , including a base 1-1, a screw seat 1-2 is fixed on the left side of the upper end of the base 1-1, a hollow cylindrical wall 2-1 is arranged on the right side of the screw seat 1-2, an exchange port 2-2 is arranged on the right side of the upper part of the hollow cylindrical wall 2-1, a second cover 4-3 is arranged on the hollow cylindrical wall 2-1 and inserted into the exchange port 2-2, so as to close the exchange port 2-2, the first cover 4-3 is pulled out upwardly to complete the opening of the exchange port 2-2;
[0029] With reference to Figures 5 to 8 , a sealing cover is arranged on the left side of the inside of the hollow cylindrical wall 2-1, the sealing cover includes a first gland 3-1, a second gland 3-2 is attached to the left end of the first gland 3-1, a bolt 3-4 passes through the first gland 3-1 from left to right, the head of the bolt 3-4 is arranged to the left and abuts against the left end of the second gland 3-2, the left end of the first gland 3-1 is provided with an internal thread hole, the tail of the bolt 3-4 is connected in the internal thread hole of the first gland 3-1 through thread cooperation, an annular groove is formed between the first gland 3-1 and the second gland 3-2, a sealing ring 3-3 is arranged in the annular groove, the outer periphery of the sealing ring 3-3 protrudes from the outer periphery of the first gland 3-1 and the second gland 3-2, so that the outer ring of the sealing ring 3-3 is attached to the inner wall of the hollow cylindrical wall 2-1 to complete the sealing of the left side of the hollow cylindrical wall 2-1, the first gland 3-1 and the second gland 3-2 are respectively extruded on the left and right ends of the sealing ring 3-3 to realize the sealing between the first gland 3-1, the second gland 3-2 and the sealing ring 3-3; the sealing ring 3-3 is an elastomer, such as medical rubber or silicone.
[0030] The left end of the second gland 3-2 is fixedly connected with a screw rod 3-5, the screw rod 3-5, the first gland 3-1, the second gland 3-2, the sealing ring 3-3 and the hollow cylindrical wall body 2-1 are coaxially arranged, the outer thread of the screw rod 3-5 is matched with the inner thread hole on the screw rod seat 1-2, the screw rod 3-5 is screwed into the inner thread hole of the screw rod seat 1-2, and the second gland 3-2 can be driven to move left and right along with the rotation of the screw rod 3-5, and then the sealing ring 3-3 is driven to move left and right in the hollow cylindrical wall body 2-1; on the other hand, the relative position of the screw rod 3-5 and the screw rod seat 1-2 is fixed by using the friction between the outer thread of the screw rod 3-5 and the inner thread of the screw rod seat 1-2, when the thread is tightened, the tooth type surface between the inner and outer threads will be extruded, thereby forming a friction force, when the screw rod 3-5 is subjected to an axial force, the friction force will hinder the relative rotation of the thread, thereby achieving the effect of self-locking, for example, between the bolt and the nut, and between the screw and the inner thread hole. The self-locking ability can be controlled by the thread rise angle and the tooth type angle. The thread rise angle is the angle between the tangent of the helix on the thread mean diameter cylinder and the plane perpendicular to the thread axis direction. When the thread rise angle decreases, the friction force component between the threads will increase, thereby enhancing the self-locking property. For single-threaded screw, since the number of threads is small, the thread rise angle is relatively small, and therefore the self-locking property is good. Compared with coarse thread, the pitch of fine thread is small, and therefore the thread rise angle is also small under the same nominal diameter, and therefore the self-locking property is better. The thread rise angle is less than or equal to the friction angle, and the self-locking is completed. Although the increase of the tooth type angle can improve the self-locking property to a certain extent, because the efficiency of the helical pair is reduced, but the tooth type angle is too large, and therefore the carrying capacity of the thread is reduced. Therefore, under the premise of ensuring the carrying capacity, appropriately reducing the tooth type angle is helpful to improve the self-locking property. The increase of the tooth side angle: the increase of the tooth side angle will increase the friction angle, and thereby enhance the self-locking property.
[0031] Further description, the inner wall of the hollow cylindrical wall body 2-1 is fixedly connected with two electrodes 2-3 located on the right side of the sealing cover, recesses can be arranged on the inner wall of the hollow cylindrical wall body 2-1, so that the electrodes 2-3 are embedded in the recesses, or the distance between the electrodes 2-3 and the first gland 3-1 is large, the purpose is to provide space for the right movement of the first gland 3-1.
[0032] The first cover 4-1 is inserted into the right part of the inner wall of the hollow cylindrical wall body 2-1, and the right part of the hollow cylindrical wall body 2-1 is closed, the left end of the first cover 4-1 is provided with a bevel 4-2, so as to be squeezed into the hollow cylindrical wall body 2-1, the bevel 4-2 gradually rises from left to right, so that after the hollow cylindrical wall body 2-1 is rotated clockwise by 90°, the bevel 4-2 realizes the drainage, and guides the liquid to the exchange port 2-2.
[0033] The upper end of the base 1-1 is provided with two sockets in front and back, the lower end of the hollow cylindrical wall 2-1 is fixedly connected with two supporting members 2-4, the two supporting members 2-4 are respectively inserted into the two sockets; when liquid needs to be discharged, the second cover 4-3 is pulled out, the first cover 4-1 serves as the bottom of the container, so that the exchange port 2-2 located at the bottom can discharge liquid. When the second pressing cover 3-2 is left shifted to be separated from the hollow cylindrical wall 2-1, the supporting member 2-4 can be pulled out from the socket, so that the whole container can be disassembled, which is beneficial to disinfection and cleaning.
[0034] The outer peripheral surface of the sealing ring 3-3 is provided with an annular groove, when the sealing ring 3-3 is pressed in the radial direction, the sealing ring 3-3 can be deformed laterally to the annular groove, so that the sealing capacity is increased.
[0035] The outer peripheral edge of the left end of the first pressing cover 3-1 and the outer peripheral edge of the right end of the second pressing cover 3-2 are both provided with chamfered surfaces, so as to be easily squeezed into the sealing ring 3-3.
[0036] The top of the inclined surface 4-2 is located at the left side of the right end edge of the exchange port 2-2, so that liquid stagnation can be avoided.
[0037] The left end of the screw rod 3-5 is provided with a prismatic groove, a handle is inserted into the prismatic groove, and the handle is rotated to facilitate the rotation of the screw rod and save labor.
Claims
1. An apparatus for electroporating a eukaryotic cell, characterized by, The base (1-1) is provided with a screw rod base (1-2), the right side of the screw rod base (1-2) is provided with a hollow cylindrical wall body (2-1), the upper right side of the hollow cylindrical wall body (2-1) is provided with an exchange port (2-2), the right end of the hollow cylindrical wall body (2-1) is provided with a first cover (4-1), the hollow cylindrical wall body (2-1) is provided with a second cover (4-3) for closing the exchange port (2-2), the left side of the inside of the hollow cylindrical wall body (2-1) is provided with a sealing cover, the left end of the sealing cover is fixedly connected with a screw rod (3-5), and the screw rod (3-5) is connected on the screw rod base (1-2) through thread cooperation.
2. An apparatus for electroporation of eukaryotic cells according to claim 1, characterized in that The inner wall of the hollow cylindrical wall body (2-1) is provided with two electrodes (2-3) located on the right side of the sealing cover.
3. The apparatus for electroporation of eukaryotic cells according to claim 1, characterized in that, The upper end of the base (1-1) is provided with two sockets, and the lower end of the hollow cylindrical wall body (2-1) is fixedly connected with two supporting pieces (2-4), which are respectively inserted into the two sockets.
4. The apparatus for electroporation of eukaryotic cells according to claim 1, characterized in that, The sealing cover comprises a first gland (3-1), the left end of the first gland (3-1) is detachably connected with a second gland (3-2), an annular groove is formed between the first gland (3-1) and the second gland (3-2), a sealing ring (3-3) is arranged in the annular groove, the first gland (3-1) and the second gland (3-2) are respectively pressed on the left and right ends of the sealing ring (3-3), and the outer circle of the sealing ring (3-3) is attached to the inner wall of the hollow cylindrical wall body (2-1).
5. An apparatus for electroporation of eukaryotic cells according to claim 4, characterised in that, A bolt (3-4) is arranged on the first gland (3-1), the head of the bolt (3-4) abuts against the left end of the second gland (3-2), and the tail of the bolt (3-4) is connected on the first gland (3-1) through thread cooperation.
6. The apparatus for electroporation of eukaryotic cells according to claim 4, characterized in that, The outer circumferential surface of the sealing ring (3-3) is provided with an annular groove.
7. The apparatus for electroporation of eukaryotic cells according to claim 4, characterized in that, The left end outer circumferential edge of the first gland (3-1) and the right end outer circumferential edge of the second gland (3-2) are both provided with a chamfer surface.
8. The apparatus for electroporation of eukaryotic cells according to claim 1, characterized in that, The first cover (4-1) is inserted into the right part of the inner wall of the hollow cylindrical wall body (2-1), the left end outer circumferential edge of the first cover (4-1) is provided with an inclined surface (4-2), and the inclined surface (4-2) gradually rises from left to right.
9. An apparatus for electroporation of eukaryotic cells according to claim 8, characterised in that, The top of the inclined surface (4-2) is located on the left side of the right end edge of the exchange port (2-2).
10. The apparatus for electroporation of eukaryotic cells according to claim 1, characterized in that, The left end of the screw rod (3-5) is provided with a prismatic groove, and a handle is inserted into the prismatic groove.