Culture kit for neural stem cell amplification

Abstract

The utility model discloses a culture kit for neural stem cell amplification, which comprises a kit body, a plurality of culture chambers with top openings are arranged in the kit body, a culture tube is inserted into each culture chamber, and a heating mechanism for conveying hot air to each culture chamber is arranged at one end of the kit body; the upper end of the culture tube is provided with an extension part extending outwards, the extension part is arranged on the box body, the diameter of an opening in the top of the culture chamber is larger than that of the culture tube, two symmetrical storage grooves are formed in the opening of the culture chamber, elastic clamping pieces are arranged in the two storage grooves and used for clamping the upper portion of the culture tube, and a swing mechanism is arranged at the bottom of the culture chamber. The utility model relates to a culture tube swinging device. Hot air is conveyed to each culture room through the heating mechanism, and independent temperature control can be performed on each culture room, so that a plurality of groups of culture tubes can be conveniently compared.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of culture kit, specifically to a culture kit for neural stem cell expansion. BACKGROUND

[0002] Neural stem cells refer to cells existing in the nervous system, having the potential to differentiate into neurons, astrocytes and oligodendrocytes, thereby being able to produce a large number of brain cell tissues and being able to self-renew and provide a large number of brain tissue cells.

[0003] During the culture of neural stem cells, the culture solution needs to be kept at a certain temperature. Through retrieval, it is found that the Chinese utility model patent with the authorized publication number CN212388045U discloses a high-efficiency hematopoietic stem cell culture kit. According to the specification, the inside of the box can be heated by an electric heating resistance wire. However, it can only heat the entire kit and cannot heat each culture area individually, thereby failing to accurately control the temperature of each culture area.

[0004] The Chinese utility model patent with the authorized publication number CN218262550U discloses an in-vitro high-efficiency culture kit for hair follicle stem cells. According to the specification, the inside of the culture test tube can be added with nutrient solution through the liquid guide pipe by opening the sealing cover. However, after adding the nutrient solution, the culture test tube needs to be slightly shaken to quickly dissolve the nutrient solution. However, if the culture kit in the above patent needs to shake the culture test tube, the test tube needs to be taken out, which is very inconvenient. UTILITY MODEL CONTENT

[0005] In view of the deficiencies of the prior art, the utility model provides a culture kit for neural stem cell expansion to solve the problems that the current culture kit cannot heat each culture tube unit and cannot be conveniently and slightly shaken.

[0006] To solve the above technical problems, the utility model provides the following technical scheme:

[0007] A culture kit for neural stem cell expansion includes a box body, a plurality of culture chambers with open tops are arranged in the box body, a culture tube is inserted into each culture chamber, a heating mechanism for delivering hot air to each culture chamber is arranged at one end of the box body, an extension part extending outward is arranged at the upper end of the culture tube, the extension part is arranged on the box body, the diameter of the open top of the culture chamber is greater than the diameter of the culture tube, two symmetrical storage grooves are arranged at the opening of the culture chamber, an elastic clamping piece is arranged in each storage groove to clamp the upper part of the culture tube, and a swinging mechanism is arranged at the bottom of the culture chamber to drive the culture tube to swing.

[0008] Preferably, the heating mechanism comprises a heating box mounted at one end of the box body, the heating box is provided with a heating area in the shape of a bucket, the heating area is provided with an electric heating wire, the heating box is provided with a delivery main pipe communicated with the heating area, a plurality of delivery branch pipes corresponding to the culture chambers are connected to the delivery main pipe, the delivery branch pipes are communicated with the corresponding culture chambers, a control valve is connected to each delivery branch pipe, an air blower is connected to the outer end of the heating box, the air outlet end of the air blower is communicated with the heating area, a temperature sensor is arranged in each culture chamber, and a box cover is buckled to the top of the box body.

[0009] The above technical scheme is heated by the electric heating wire, hot air is blown to the delivery main pipe by the air blower, and then is delivered to each delivery branch pipe, and then the hot air is delivered to the corresponding culture chamber by opening the corresponding control valve, so as to keep the corresponding culture tube warm, and when the required temperature is reached, the control valve is closed, or the electric heating wire and the air blower stop working.

[0010] Preferably, the elastic clamping piece comprises a spring connected in the storage groove and clamping blocks connected to the outer end of the spring, and the opposite surfaces of the two clamping blocks are arc-shaped.

[0011] The above technical scheme inserts the culture tube into the culture chamber, and through the action of the spring, the clamping blocks are clamped on both sides of the culture tube, so that the culture tube maintains a certain stability, when the lower end of the culture tube is pushed, the culture tube will press the clamping blocks and compress the spring, so as to realize the slight and balanced shaking of the culture tube.

[0012] Preferably, the swinging mechanism comprises a lead screw rotatably connected in the culture chamber, guide rods connected in the culture chamber and located on both sides of the lead screw, a sliding block threadedly connected to the lead screw, a support block connected to the sliding block, a driving cavity arranged on one side of the culture chamber, and a transmission rod rotatably connected to the box body, the sliding block is slidably connected with the guide rod, the top of the support block is provided with a groove matched with the bottom of the culture tube, one end of the lead screw extends into the driving cavity, and the inner end of the transmission rod extends into the driving cavity, the swinging mechanism further comprises a first bevel gear connected to the inner end of the transmission rod, a second bevel gear connected to one end of the lead screw, and a handle connected to the outer end of the transmission rod, and the first bevel gear is engaged with the second bevel gear.

[0013] The above technical scheme rotates the handle to drive the transmission rod to rotate, and through the cooperation of the first bevel gear and the second bevel gear, the lead screw is driven to rotate, and the sliding block moves on the guide rod in the process of rotating the lead screw, thereby driving the support block to move, since the diameter of the opening at the upper end of the culture chamber is greater than the diameter of the culture tube, when the support block drives the culture tube to move, the spring on one side is compressed, and then the handle is reversely rotated to drive the culture tube to move reversely, thereby realizing the left and right swinging of the lower end of the culture tube, so that the culture solution is fully dissolved.

[0014] Preferably, the arc-shaped surface of the clamping block is connected with a rubber pad.

[0015] The above technical scheme sets rubber pads on the inner wall of the clamping block, so that the clamping block can prevent the culture tube from being abraded.

[0016] Preferably, one side of the box body is provided with a control box, the control box is provided with a controller, the control box is provided with a power cord electrically connected with the controller; the box body is provided with a temperature display screen on the front wall of each culture chamber; the temperature display screen, the temperature sensor, the control valve, the air blower and the electric heating wire are electrically connected with the controller.

[0017] The above technical scheme is provided with a control program in the controller, which is used for controlling the orderly work of each component, such as controlling the opening and closing of the control valve, controlling the connection or closing of the electric heating wire, and controlling the opening or closing of the air blower.

[0018] Preferably, the inner wall of the culture chamber is provided with a heat preservation layer.

[0019] The above technical scheme can heat preservation of the culture chamber through the heat preservation layer.

[0020] Compared with the prior art, the utility model has the following beneficial effects:

[0021] The heating mechanism can deliver hot air to each culture chamber, so that the temperature of each culture chamber can be controlled individually, and multiple groups of culture tubes can be compared.

[0022] After the nutrient solution is added into the culture tube, the swinging mechanism can drive the culture tube to swing, so that the culture tube is shaken and the nutrient solution is fully dissolved, and the culture tube does not need to be taken out, thereby improving the convenience of culture. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a schematic view of the utility model;

[0024] Figure 2 It is a sectional view of the utility model;

[0025] Figure 3 It is Figure 2 the enlarged view of A of the utility model;

[0026] Figure 4 It is Figure 2 the enlarged view of B of the utility model;

[0027] Figure 5 It is a schematic view of the clamping block;

[0028] In the figure: 1-box, 2-culture chamber, 3-culture tube, 4-extension, 5-putting groove, 6-heating box, 7-heating area, 8-electric heating wire, 9-delivery main pipe, 10-delivery branch pipe, 11-control valve, 12-blower, 13-temperature sensor, 14-box cover, 15-spring, 16-clamping block, 17-screw rod, 18-guide rod, 19-sliding block, 20-supporting block, 21-driving cavity, 22-transmission rod, 23-first bevel gear, 24-second bevel gear, 25-handle, 26-rubber pad, 27-control box, 28-controller, 29-temperature display screen, 30-heat preservation layer. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0030] Embodiment 1

[0031] Please refer to Figures 1-5 A culture kit for neural stem cell expansion comprises a box 1, a plurality of culture chambers 2 with open top are arranged in the box 1, a culture tube 3 is arranged in each culture chamber 2, and a heating mechanism for delivering hot air to each culture chamber is arranged at one end of the box 1. Specifically, the heating mechanism comprises a heating box 6 arranged at one end of the box, a heating area 7 in the shape of a bucket is arranged in the heating box 6, an electric heating wire 8 is arranged in the heating area 7, a delivery main pipe 9 in communication with the heating area is arranged in the heating box 6, a plurality of delivery branch pipes 10 corresponding to the culture chambers are connected to the delivery main pipe 9, the delivery branch pipes 10 are in communication with the corresponding culture chambers 2, a control valve 11 is connected to each delivery branch pipe 10, a blower 12 is connected to the outer end of the heating box 6, the air outlet end of the blower 12 is in communication with the heating area 7, a temperature sensor 13 is arranged in each culture chamber 2, a box cover 14 is buckled to the top of the box 1, specifically, a clamping rod is arranged at the top of the box, and a clamping hole matched with the clamping rod is arranged on the bottom surface of the box cover. The culture kit in the embodiment needs to be used in cooperation with an air purification device, that is, the purified air is blown into the heating area 7 by the blower 12, at this time, the electric heating wire 8 works to heat the purified air, the hot air is blown to the delivery main pipe 9 by the blower 12, and then the hot air is delivered to each delivery branch pipe 10 by the delivery main pipe 9, then the corresponding control valve 11 is opened, so that the hot air can be delivered to the corresponding culture chamber 2, to heat the corresponding culture tube 3, when the required temperature is reached, the control valve 11 is closed, or the electric heating wire 8 and the blower 12 stop working.

[0032] The upper end of the culture tube 3 is provided with an outwardly extending extension 4, and the extension 4 is placed on the box body 1. The diameter of the opening at the top of the culture chamber 2 is larger than the diameter of the culture tube 3. The opening of the culture chamber 2 is provided with two symmetrical storage grooves 5. The two storage grooves 5 are each provided with an elastic clamping piece for clamping the upper part of the culture tube 3. The elastic clamping piece includes a spring 15 connected to the storage groove, and a clamping block 16 connected to the outer end of the spring. The opposite surfaces of the two clamping blocks 16 are arc-shaped. The arc-shaped surface of the clamping block 16 is connected with a rubber pad 26. After the culture tube 3 is inserted into the culture chamber 2, the clamping block 16 is clamped on both sides of the culture tube by the action of the spring 15, and the rubber pad 26 directly contacts the culture tube 3, which can effectively prevent the culture tube from being worn. The clamping block 16 can also make the culture tube stable. When the lower end of the culture tube 3 is pushed, the culture tube 3 will press the clamping block 16 and compress the spring 15, so as to realize the slight and balanced shaking of the culture tube 3.

[0033] The bottom of the culture chamber 2 is provided with an oscillating mechanism for driving the lower end of the culture tube 3 to swing. Specifically, the oscillating mechanism includes a lead screw 17 rotatably connected to the culture chamber, a guide rod 18 connected to the culture chamber and located on both sides of the lead screw, a sliding block 19 threadedly connected to the lead screw, a support block 20 connected to the sliding block, a drive cavity 21 provided on one side of the culture chamber, and a transmission rod 22 rotatably connected to the box body. The sliding block 19 is slidably connected to the guide rod 18. The top of the support block 20 is provided with a groove matching the bottom of the culture tube 3. One end of the lead screw 17 extends into the drive cavity 21, and the inner end of the transmission rod 22 extends into the drive cavity 21. The oscillating mechanism further includes a first bevel gear 23 connected to the inner end of the transmission rod, a second bevel gear 24 connected to one end of the lead screw, and a handle 25 connected to the outer end of the transmission rod. The first bevel gear 23 is engaged with the second bevel gear 24. Rotating any handle 25 drives the corresponding transmission rod 22 to rotate. Through the cooperation of the first bevel gear 23 and the second bevel gear 24, the lead screw 17 is driven to rotate. During the rotation of the lead screw 17, the sliding block 19 moves on the guide rod 18, thereby driving the support block 20 to move. Since the diameter of the opening at the top of the culture chamber 2 is larger than the diameter of the culture tube 3, a certain gap is maintained between the culture tube 3 and the opening of the culture chamber 2. When the support block 20 drives the culture tube 3 to move, the spring 15 on one side is compressed, and the spring 15 on the other side is extended. The culture tube is kept stable by the two clamping blocks 16. Reversely rotating the handle 25 drives the culture tube 3 to move reversely, thereby realizing the left and right swinging of the culture tube, so as to make the culture solution fully dissolve.

[0034] The side of the box body 1 is provided with a control box 27, the control box 27 is provided with a controller 28, the control box 27 is provided with a power line electrically connected with the controller; the front wall of each culture chamber of the box body 1 is provided with a temperature display screen 29, the temperature display screen 29, the temperature sensor 13, the control valve 11, the blower 12 and the electric heating wire 8 are electrically connected with the controller 28. The controller is written with a control program for controlling the orderly work of each component, such as controlling the opening and closing of the control valve, so as to control the opening or closing of any conveying branch pipe, so as to facilitate the conveying of hot air to any culture chamber; controlling the connection or closing of the electric heating wire, so as to realize heating or stopping heating; controlling the opening or closing of the blower, so as to control blowing or not blowing.

[0035] The working principle of the embodiment is as follows:

[0036] The culture tube 3 is inserted into the culture chamber 2, and the box cover 14 is covered, the temperature in each culture chamber 2 is observed through the temperature display screen 29, and the temperature sensor 13 monitors the temperature in the culture chamber, when the temperature in the culture chamber is too low, the heating mechanism is used to convey hot air to the culture chamber, and the temperature in the culture chamber is controlled, when the temperature reaches the required temperature, the heating of the culture chamber is stopped.

[0037] When the nutrient solution is added to the culture tube, the corresponding handle 25 is rotated back and forth, the transmission rod 22 is rotated, and the supporting block 20 is moved left and right, the supporting block 20 drives the culture tube 3 to move left and right, so as to realize the left and right swing of the culture tube, so that the culture solution is fully dissolved.

[0038] Example 2

[0039] On the basis of example 1, the inner wall of the culture chamber 2 is provided with a heat preservation layer 30. The heat preservation layer can be used to heat preservation of the culture chamber, and the energy consumption is reduced.

[0040] It should be noted that the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment. Without more limitation, the element defined by the statement "including a" does not exclude the presence of another identical element in the process, method, article or equipment including the element.

[0041] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A culture kit for neural stem cell expansion, characterized by: The utility model provides a culture box, including box (1), be equipped with a plurality of top open culture chamber (2) in box (1), insert culture tube (3) in each culture chamber (2), one end of box (1) is equipped with the heating mechanism that transports hot air to each culture chamber, the upper end of culture tube (3) is equipped with the extension (4) that extends outward, and extension (4) is placed on box (1), and the diameter of culture chamber (2) top opening is greater than the diameter of culture tube (3), and the opening of culture chamber (2) is equipped with two symmetrical storage groove (5), and the elastic clamping piece is arranged in two storage groove (5) and is used for clamping the upper portion of culture tube (3), and the bottom of culture chamber (2) is equipped with swing mechanism and is used for driving culture tube (3) swing.

2. The culture kit for expansion of neural stem cells according to claim 1, wherein: The heating mechanism includes a heating box (6) mounted on one end of the box, the heating box (6) is provided with a heating area (7) in the shape of a bucket, the heating area (7) is provided with an electric heating wire (8), the heating box (6) is provided with a delivery main pipe (9) in communication with the heating area, the delivery main pipe (9) is connected with a plurality of delivery branch pipes (10) corresponding to the culture chambers, the delivery branch pipes (10) are in communication with the corresponding culture chambers (2), each delivery branch pipe (10) is connected with a control valve (11), the outer end of the heating box (6) is connected with a blower (12), the air outlet end of the blower (12) is in communication with the heating area (7), each culture chamber (2) is provided with a temperature sensor (13), and the top of the box (1) is buckled with a box cover (14).

3. The culture kit for expansion of neural stem cells according to claim 2, wherein: The elastic clamping piece includes a spring (15) connected in the storage groove, a clamping block (16) connected to the outer end of the spring, and the opposite surfaces of the two clamping blocks (16) are arc-shaped.

4. The culture kit for expansion of neural stem cells according to claim 3, wherein: The swing mechanism includes a lead screw (17) rotatably connected in the culture chamber, a guide rod (18) connected to the culture chamber and located on both sides of the lead screw, a sliding block (19) threadedly connected to the lead screw, a support block (20) connected to the sliding block, a drive cavity (21) provided on one side of the culture chamber, and a transmission rod (22) rotatably connected to the box, the sliding block (19) is slidably connected with the guide rod (18), the top of the support block (20) is provided with a groove matched with the bottom of the culture tube (3), one end of the lead screw (17) extends into the drive cavity (21), the inner end of the transmission rod (22) extends into the drive cavity (21), and the swing mechanism further includes a first bevel gear (23) connected to the inner end of the transmission rod, a second bevel gear (24) connected to one end of the lead screw, and a handle (25) connected to the outer end of the transmission rod, the first bevel gear (23) is engaged with the second bevel gear (24).

5. The culture kit for expansion of neural stem cells according to claim 4, wherein: The arc-shaped surface of the clamping block (16) is connected with a rubber pad (26).

6. The culture kit for expansion of neural stem cells according to claim 5, wherein: One side of the box (1) is provided with a control box (27), the control box (27) is provided with a controller (28), and the control box (27) is provided with a power line electrically connected with the controller; the front wall of each culture chamber of the box (1) is provided with a temperature display screen (29), and the temperature display screen (29), the temperature sensor (13), the control valve (11), the blower (12), and the electric heating wire (8) are electrically connected with the controller (28).

7. A culture kit for expansion of neural stem cells according to any one of claims 1 to 6, characterized in that: The inner wall of the culture room (2) is provided with a heat preservation layer (30).

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