Extraction device for nucleic acid extraction equipment and nucleic acid extraction equipment

Abstract

The application discloses an extraction device for nucleic acid extraction equipment and the nucleic acid extraction equipment, the extraction device comprises: a bottom plate on which a mounting area and a sample area are formed side by side along the width direction; the first driving part is arranged in the mounting area and is distributed in the length direction of the bottom plate; the magnetic bar sleeve assembly is arranged above the sample area and can vertically move, and the magnetic bar sleeve assembly is in driving connection with the first driving part; the second driving part is arranged above the first driving part, the arrangement direction of the second driving part is consistent with the length direction of the bottom plate, and the second driving part and the first driving part are overlapped in the width direction of the bottom plate; the magnetic bar is arranged above the sample area and can vertically move, and the magnetic bar is in driving connection with the second driving part and can partially extend into the magnetic bar sleeve assembly or be separated from the magnetic bar sleeve assembly under the driving action of the second driving part. The extraction device has the advantages of being compact and reasonable in layout and beneficial to reducing the volume of the extraction device and the nucleic acid extraction equipment.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of nucleic acid extraction equipment, and particularly relates to an extraction device for a nucleic acid extraction equipment and the nucleic acid extraction equipment. BACKGROUND

[0002] The nucleic acid extraction equipment (such as a nucleic acid extraction instrument) belongs to the field of molecular detection equipment and is widely applied in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, animal husbandry and molecular biology research. The extraction device of the existing nucleic acid extraction equipment is not reasonable in the arrangement and layout of internal components, resulting in a large volume of the extraction device, and further resulting in a large space occupied by the nucleic acid extraction equipment, which is inconvenient for transportation or direct placement in a biological safety cabinet. CONTENT OF THE UTILITY MODEL

[0003] The purpose of the present application is to provide an extraction device for a nucleic acid extraction equipment and the nucleic acid extraction equipment, which have the advantages of compact and reasonable layout, and are beneficial to reducing the volume of the extraction device and the nucleic acid extraction equipment.

[0004] In order to achieve the above-mentioned purpose, the first aspect of the present application provides an extraction device for a nucleic acid extraction equipment, which comprises:

[0005] A bottom plate, wherein mounting areas and sample areas are formed on the bottom plate in a parallel arrangement along the width direction;

[0006] A first driving member arranged in the mounting areas and distributed along the length direction of the bottom plate;

[0007] A magnetic rod sleeve assembly arranged above the sample areas and vertically movable, wherein the magnetic rod sleeve assembly is drivingly connected with the first driving member;

[0008] A second driving member arranged above the first driving member, wherein the arrangement direction of the second driving member is consistent with the length direction of the bottom plate, and the second driving member overlaps the first driving member in the width direction of the bottom plate;

[0009] A magnetic rod arranged above the sample areas and vertically movable, wherein the magnetic rod is drivingly connected with the second driving member and can be partially inserted into the interior of the magnetic rod sleeve assembly or separated from the magnetic rod sleeve assembly under the driving action of the second driving member.

[0010] In the embodiments of the present application, the extraction device further comprises:

[0011] A vertical plate arranged above the bottom plate in the vertical direction, wherein the first driving member is arranged at the bottom end of the vertical plate, and the second driving member is arranged at the top end of the vertical plate;

[0012] The first synchronous belt assembly is arranged vertically on one side of the vertical plate and is drivingly connected with the first driving member. A first synchronous belt of the first synchronous belt assembly is connected with the magnetic rod sleeve assembly.

[0013] The second synchronous belt assembly is arranged vertically on a side of the vertical plate away from the first synchronous belt assembly and is drivingly connected with the second driving member. A second synchronous belt of the second synchronous belt assembly is connected with the magnetic rod.

[0014] In the embodiment of the present application, a mounting space for mounting the tensioning mechanism is formed on the vertical plate, and the mounting space is located between the first driving member and the second driving member.

[0015] In the embodiment of the present application, the extraction device further comprises:

[0016] The first guide rail is arranged vertically on a side wall of the vertical plate close to the sample area.

[0017] The first slider is movably arranged on the first guide rail. A first connecting portion connected with the first synchronous belt and a first mounting portion for mounting the magnetic rod sleeve assembly are formed on the first slider.

[0018] The second slider is movably arranged on the first guide rail and located above the first slider. A second connecting portion connected with the second synchronous belt and a second mounting portion for mounting the magnetic rod are formed on the second slider.

[0019] In the embodiment of the present application, the magnetic rod sleeve assembly comprises:

[0020] The magnetic rod sleeve mounting member is arranged on the first mounting portion. A plug hole for the magnetic rod to pass through is formed on the magnetic rod sleeve mounting member.

[0021] The magnetic rod sleeve is arranged in the reagent tube and is detachably connected with the magnetic rod sleeve mounting member.

[0022] In the embodiment of the present application, the plug hole is funnel-shaped.

[0023] In the embodiment of the present application, an end of the magnetic rod away from the second mounting portion is formed with a guide portion.

[0024] In the embodiment of the present application, the extraction device further comprises a first reset assembly for resetting the second driving member. The first reset assembly comprises:

[0025] The first blocking piece is arranged on the second slider.

[0026] The first sensing member is arranged on the vertical plate and located on the same side as the second synchronous belt assembly, and is used for detecting the first blocking piece.

[0027] In the embodiment of the present application, the extraction device further comprises a side plate vertically arranged on one side of the vertical plate close to the sample area, and a second reset assembly for resetting the first driving member, the second reset assembly comprising:

[0028] A second blocking piece is arranged on the first sliding block and located on the vertical side wall of the first sliding block close to the side plate;

[0029] A second sensing member is arranged on the side plate and used for detecting the second blocking piece.

[0030] The second aspect of the present application provides a nucleic acid extraction device, which comprises the above-mentioned extraction device for the nucleic acid extraction device.

[0031] According to the above technical solution, the extraction device comprises a bottom plate, a first driving member, a magnetic rod sleeve assembly, a second driving member and a magnetic rod. The bottom plate is formed with a mounting area and a sample area arranged side by side in the width direction. The first driving member is arranged in the mounting area and is distributed along the length direction of the bottom plate. The magnetic rod sleeve assembly is arranged above the sample area and is vertically movable. The magnetic rod sleeve assembly is drivingly connected with the first driving member. The second driving member is arranged above the first driving member and is arranged in the same direction as the length direction of the bottom plate. In the width direction of the bottom plate, the second driving member overlaps the first driving member. The magnetic rod is arranged above the sample area and is vertically movable. The magnetic rod is drivingly connected with the second driving member and can be partially inserted into the interior of the magnetic rod sleeve assembly or separated from the magnetic rod sleeve assembly under the driving action of the second driving member. The extraction device has a simple structure. Since the first driving member and the second driving member are vertically spaced and overlap in the width direction of the bottom plate, the overall width of the first driving member and the second driving member is consistent with the width of the first driving member (or the width of the second driving member), which is conducive to reducing the overall width of the extraction device. Since the arrangement direction of the first driving member and the arrangement direction of the second driving member are both along the length direction of the bottom plate, the width occupied by the first driving member and the second driving member in the extraction device can be further reduced, which makes the layout of the components of the extraction device more compact, is conducive to reducing the volume of the extraction device and the nucleic acid extraction device, is conducive to realizing the miniaturization design of the extraction device and the nucleic acid extraction device, and is conducive to enabling the nucleic acid extraction device to be directly placed in a biological safety cabinet.

[0032] Other features and advantages of the present application will be described in detail in the following specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0033] The accompanying drawings are included to provide a further understanding of embodiments of the application, and are incorporated in and constitute a part of this specification, illustrate embodiments of the application, and together with the description serve to explain them. Other embodiments of the application can be obtained from a combination of these drawings with the description, which forms a part of the specification. In the drawings:

[0034] Figure 1 Structure diagram of the first perspective of the extraction device in the embodiment of the application;

[0035] Figure 2 Structure diagram of the second perspective of the extraction device in the embodiment of the application;

[0036] Figure 3 Structure diagram of the third perspective of the extraction device in the embodiment of the application;

[0037] Figure 4 Structure diagram of the magnetic rod sleeve mounting member in the embodiment of the application;

[0038] Figure 5 Structure diagram of the second sliding block and the magnetic rod in the embodiment of the application;

[0039] Figure 6 Structure diagram of the first sliding block and the magnetic rod in the embodiment of the application.

[0040] Legend of reference signs

[0041] 1 - base plate; 101 - mounting area; 102 - sample area; 2 - first driving member; 3 - magnetic rod sleeve assembly; 301 - magnetic rod sleeve mounting member; 302 - insertion hole; 303 - magnetic rod sleeve; 304 - annular groove; 4 - second driving member; 5 - magnetic rod; 501 - guide portion; 6 - vertical plate; 601 - mounting space; 7 - first synchronous belt assembly; 701 - first driving wheel; 702 - first synchronous belt; 703 - first driven wheel; 8 - second synchronous belt assembly; 801 - second driving wheel; 802 - second synchronous belt; 803 - second driven wheel; 9 - first guide rail; 10 - first sliding block; 1001 - first connecting portion; 1002 - first mounting portion; 1003 - third connecting portion; 11 - second sliding block; 1101 - second connecting portion; 1102 - second mounting portion; 1103 - fourth connecting portion; 12 - first reset assembly; 1201 - first blocking piece; 1202 - first sensing member; 13 - side plate; 14 - second reset assembly; 1401 - second blocking piece; 1402 - second sensing member. DETAILED DESCRIPTION

[0042] The specific embodiments of the present application are described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative and explanatory and are not intended to limit the present application.

[0043] Embodiments of the present application provide an extraction device for a nucleic acid extraction apparatus, as shown in the drawings, the extraction device comprises: Figures 1-3

[0044] a bottom plate 1, the bottom plate 1 is formed with installation areas 101 and sample areas 102 distributed side by side along the width direction;

[0045] a first driving member 2 arranged in the installation areas 101 and distributed along the length direction of the bottom plate 1;

[0046] a magnetic rod sleeve assembly 3 arranged above the sample areas 102 and vertically movable, the magnetic rod sleeve assembly 3 is drivingly connected with the first driving member 2;

[0047] a second driving member 4 arranged above the first driving member 2, the arrangement direction of the second driving member 4 is consistent with the length direction of the bottom plate 1, and in the width direction of the bottom plate 1, the second driving member 4 overlaps with the first driving member 2;

[0048] a magnetic rod 5 arranged above the sample areas 102 and vertically movable, the magnetic rod 5 is drivingly connected with the second driving member 4 and can be partially inserted into the inside of the magnetic rod sleeve assembly 3 or separated from the magnetic rod sleeve assembly 3 under the driving action of the second driving member 4.

[0049] Specifically, the nucleic acid extraction apparatus in the embodiments can be a nucleic acid extraction instrument, the installation areas 101 and the sample areas 102 are distributed in parallel and spaced apart along the width direction of the bottom plate 1 on the bottom plate 1, and the installation areas 101 and the sample areas 102 both extend along the length direction of the bottom plate 1. The nucleic acid extraction apparatus further comprises a second guide rail, a carrier and reagent tubes, the second guide rail is arranged in the sample areas 102, the carrier is movably arranged on the second guide rail, the number of the reagent tubes is multiple, and the multiple reagent tubes are all detachably arranged on the carrier. Different types of solutions such as lysis solution, buffer solution, etc. are stored in the multiple reagent tubes, and magnetic beads capable of adsorbing nucleic acids are also pre-placed in the reagent tubes.

[0050] The first driving member 2 and the second driving member 4 in the embodiments can both be selected as a stepper motor, under the driving action of the first driving member 2, the magnetic rod sleeve assembly 3 moves up and down above the sample areas 102; under the driving action of the second driving member 4, the magnetic rod 5 can move up and down above the sample areas 102. By changing the movement speed of the first driving member 2 and the second driving member 4 respectively, the magnetic rod sleeve assembly 3 and the magnetic rod 5 can move alone or together.

[0051] ​Before nucleic acid extraction, the partial magnetic rod sleeve assembly 3 is pre-inserted into the reagent tube which needs to be extracted, and then the second driving element 4 is controlled to move, so that the magnetic rod 5 moves downward until the magnetic rod 5 moves to the position, and after the magnetic rod 5 moves to the position, the partial magnetic rod 5 is inserted into the inside of the magnetic rod sleeve assembly 3, so as to pick up the magnetic beads in a reagent tube; after the magnetic beads are picked up, the first driving element 2 and the second driving element 4 are controlled to rotate together, so that the magnetic rod 5 and the magnetic rod sleeve assembly 3 (the magnetic rod sleeve assembly 3 has the magnetic beads adsorbed thereon, and the nucleic acid is adsorbed on the magnetic beads) move upward at the same speed, and after the magnetic rod sleeve assembly 3 is separated from the reagent tube, the moving carrier is moved to move another reagent tube to the position directly below the magnetic rod sleeve assembly 3; after the carrier moves to the position, the first driving element 2 and the second driving element 4 are controlled to rotate together again, so that the magnetic rod 5 and the magnetic rod sleeve assembly 3 (the magnetic rod sleeve assembly 3 has the magnetic beads adsorbed thereon, and the nucleic acid is adsorbed on the magnetic beads) move downward at the same speed, and then the magnetic rod sleeve assembly 3 with the magnetic beads adsorbed therein is inserted into the reagent tube below; after the magnetic rod 5 and the magnetic rod sleeve assembly 3 move downward to the position, the second driving element 4 is controlled to rotate, and then the magnetic rod 5 moves upward and away from the magnetic rod sleeve assembly 3, so as to release the magnetic beads and wash the nucleic acid.

[0052] The extraction device in the embodiment has simple structure, the first driving element 2 and the second driving element 4 are vertically spaced, and the second driving element 4 overlaps the first driving element 2 in the width direction of the bottom plate 1, so that the overall width of the first driving element 2 and the second driving element 4 is consistent with the width of the first driving element 2 (or the width of the second driving element 4), which is conducive to reducing the overall width of the extraction device; and since the setting direction of the first driving element 2 and the setting direction of the second driving element 4 are both along the length direction of the bottom plate 1, the width occupied by the first driving element 2 and the second driving element 4 in the extraction device can be further reduced, so that the layout between the parts of the extraction device is more compact, which is conducive to reducing the volume of the extraction device and the nucleic acid extraction equipment, facilitating the miniaturization design of the extraction device and the nucleic acid extraction equipment, and enabling the nucleic acid extraction equipment to be directly placed in a biological safety cabinet.

[0053] Further, the magnetic flux of the magnetic rod 5 in the embodiment is 5000 Gauss, and the magnetic rod 5 with the magnetic flux can effectively adsorb the nanoscale magnetic beads in the reagent tube, thereby achieving the effect of highly extracting nucleic acid.

[0054] In an embodiment of the present application, the extraction device further comprises:

[0055] The vertical plate 6 is vertically arranged above the bottom plate 1, the first driving element 2 is arranged at the bottom end of the vertical plate 6, and the second driving element 4 is arranged at the top end of the vertical plate 6;

[0056] The first synchronous belt assembly 7 is vertically arranged on one side of the vertical plate 6 and is drivingly connected with the first driving element 2, and the first synchronous belt 702 of the first synchronous belt assembly 7 is connected with the magnetic rod sleeve assembly 3.

[0057] A second synchronous belt assembly 8 is arranged vertically on the side of the vertical plate 6 away from the first synchronous belt assembly 7 and is drivingly connected with the second driving member 4, and a second synchronous belt 802 of the second synchronous belt assembly 8 is connected with the magnetic rod 5.

[0058] Specifically, the vertical plate 6 is formed with a first mounting hole and a second mounting hole at the bottom end and the top end respectively, the second mounting hole is directly above the first mounting hole, the first synchronous belt assembly 7 includes a first driving wheel 701, a first driven wheel 703 and a first synchronous belt 702, the first driving wheel 701 is arranged on the driving end of the first driving member 2 passing through the first mounting hole, the first driven wheel 703 is arranged above the first driving wheel 701 at intervals, the first synchronous belt 702 is sleeved outside the first driving wheel 701 and the first driven wheel 703, when the first driving member 2 rotates, the first synchronous belt 702 is driven to rotate, and in turn drives the magnetic rod sleeve 303 to move up and down; similarly, the second synchronous belt assembly 8 includes a second driving wheel 801, a second driven wheel 803 and a second synchronous belt 802, the second driving wheel 801 is arranged on the driving end of the second driving member 4 passing through the second mounting hole, the second driven wheel 803 is arranged above the second driving wheel 801 at intervals, the second synchronous belt 802 is sleeved outside the second driving wheel 801 and the second driven wheel 803, when the second driving member 4 rotates, the second synchronous belt 802 is driven to rotate, and in turn drives the magnetic rod 5 to move up and down. The arrangement of the first synchronous belt 702 and the second synchronous belt 802 can reduce the noise of the extraction device during use, and can effectively improve the use experience of the extraction device and the nucleic acid extraction instrument.

[0059] Further, the first synchronous belt assembly 7 and the second synchronous belt assembly 8 are respectively arranged on the two sides of the vertical plate 6, the first synchronous belt assembly 7 is arranged below the second driving member 4, and the second synchronous belt assembly 8 is arranged above the first driving member 2, the above arrangement fully utilizes the space below the second driving member 4, and further improves the compactness of the layout between the parts of the extraction device.

[0060] In an embodiment of the present application, the vertical plate 6 is formed with a mounting space 601 for mounting a tensioning mechanism, and the mounting space 601 is located between the first driving member 2 and the second driving member 4.

[0061] Specifically, the nucleic acid extraction device further includes a tensioning mechanism for tensioning the first synchronous belt 702 and / or the second synchronous belt 802, which is beneficial to ensure the accuracy of the up-down movement of the magnetic rod 5 and the magnetic rod sleeve 303. In this embodiment, the tensioning mechanism is arranged in the mounting space 601 and located between the first synchronous belt assembly 7 and the second synchronous belt assembly 8, which further improves the utilization rate of the internal space of the extraction device and makes the multiple parts of the extraction device more compact.

[0062] In an embodiment of the present application, the extraction device further comprises:

[0063] The first guide rail 9 is vertically arranged on the side wall of the stand 6 close to the sample area 102.

[0064] The first slider 10 is movably arranged on the first guide rail 9, and the first slider 10 is formed with a first connecting part 1001 connected with the first synchronous belt 702 and a first mounting part 1002 for mounting the magnetic rod sleeve assembly 3.

[0065] The second slider 11 is movably arranged on the first guide rail 9 and above the first slider 10, and the second slider 11 is formed with a second connecting part 1101 connected with the second synchronous belt 802 and a second mounting part 1102 for mounting the magnetic rod 5.

[0066] Specifically, as shown in the first embodiment of the present application, Figure 6 the first slider 10 further comprises a third connecting part 1003 vertically and movably arranged on the first guide rail 9, one end of the first connecting part 1001 is connected with the first synchronous belt 702, the other end of the first connecting part 1001 is connected with the third connecting part 1003, the first mounting part 1002 is in the form of a flat plate and is in L-shaped distribution with the third connecting part 1003, when the first driving part 2 rotates, the first synchronous belt 702 rotates, the first synchronous belt 702 drives the first slider 10 as a whole to move up and down along the first guide rail 9 through the first connecting part 1001, and the first slider 10 drives the magnetic rod sleeve assembly 3 to move up and down synchronously; as shown in the second embodiment of the present application, Figure 5 likewise, the second slider 11 further comprises a fourth connecting part 1103 vertically and movably arranged on the first guide rail 9, one end of the second connecting part 1101 is connected with the second synchronous belt 802, the other end of the second connecting part 1101 is connected with the fourth connecting part 1103, the second mounting part 1102 is in the form of a flat plate and is in L-shaped distribution with the fourth connecting part 1103, when the second driving part 4 rotates, the second synchronous belt 802 rotates, the second synchronous belt 802 drives the second slider 11 as a whole to move up and down along the first guide rail 9 through the second connecting part 1101, and the second slider 11 drives the magnetic rod 5 to move up and down synchronously. The above-mentioned structure is simple, which is conducive to reducing the production and manufacturing cost of the extraction device and the nucleic acid extraction equipment as a whole.

[0067] In an embodiment of the present application, the magnetic rod sleeve assembly 3 comprises:

[0068] The magnetic rod sleeve mounting part 301 is arranged on the first mounting part 1002, and the magnetic rod sleeve mounting part 301 is formed with a plug hole 302 for the magnetic rod 5 to pass through;

[0069] The magnetic rod sleeve 303 is arranged in the reagent tube and is detachably connected with the magnetic rod sleeve mounting part 301.

[0070] Specifically, the magnetic rod sleeve mounting member 301 in the embodiment can be selected as a magnetic rod sleeve 303 adapter and detachably connected with the first mounting portion 1002 through a bolt assembly. The bottom end of the magnetic rod sleeve mounting member 301 penetrates the first mounting portion 1002 downward. The interior of the magnetic rod sleeve 303 forms an accommodating cavity. A plurality of protrusions are formed on the inner cavity wall of the accommodating cavity and are spaced apart along the circumference of the accommodating cavity. An annular groove 304 for accommodating the plurality of protrusions is formed on the outer circumferential wall of the magnetic rod sleeve mounting member 301. The magnetic rod sleeve 303 is previously placed in the reagent tube. After the reagent tube with the pre-placed magnetic rod sleeve 303 moves into the interior of the nucleic acid extraction device along the carrier, the reagent tube with the pre-placed magnetic rod sleeve 303 moves to the position directly below the magnetic rod sleeve mounting member 301. The first driving member 2 drives the first sliding block 10 to move downward with the magnetic rod sleeve mounting member 301. After the magnetic rod sleeve mounting member 301 moves downward to the position, part of the magnetic rod sleeve mounting member 301 extends into the accommodating cavity of the magnetic rod sleeve 303. The protrusions are clamped into the annular groove 304. In this way, the connection between the magnetic rod sleeve 303 and the magnetic rod sleeve mounting member 301 is achieved.

[0071] In an embodiment of the present application, the insertion hole 302 is funnel-shaped, the insertion hole 302 is wide at the top and narrow at the bottom, and a guide section is formed at the top, which facilitates the magnetic rod 5 to quickly and smoothly pass through the magnetic rod sleeve mounting member 301, and is beneficial to improve the smoothness of the extraction device.

[0072] In an embodiment of the present application, the end of the magnetic rod 5 away from the second mounting portion 1102 forms a guide portion 501, which is in the shape of an inverted circular table, which is beneficial to further improve the smoothness of the magnetic rod 5 passing through the magnetic rod sleeve mounting member 301 downward.

[0073] In an embodiment of the present application, the extraction device further comprises a first reset assembly 12 for resetting the second driving member 4, and the first reset assembly 12 comprises:

[0074] A first blocking piece 1201 is arranged on the second sliding block 11.

[0075] A first sensing member 1202 is arranged on the vertical plate 6 and located on the same side as the second synchronous belt assembly 8, and is used for detecting the first blocking piece 1201.

[0076] Specifically, the extraction device further comprises a processor in communication with the first sensing member 1202 and the second driving member 4, the first blocking piece 1201 is arranged on the vertical side wall of the second sliding block 11 close to the second synchronous belt assembly 8, the first sensing member 1202 can be an optoelectronic sensing member and is in communication with the second driving member 4, when the second sliding block 11 moves to the position, the first blocking piece 1201 extends into the first detection groove of the first sensing member 1202, the potential of the first sensing member 1202 changes and sends the change to the processor, after the processor receives the change, it can be determined that the first sensing member 1202 detects the first blocking piece 1201, and then the processor controls the second driving member 4 to reset, the above arrangement ensures the accuracy of the movement of the second driving member 4 and the magnetic rod 5, and further ensures the accuracy of the extraction device when performing the extraction operation.

[0077] In an embodiment of the present application, the extraction device further comprises a side plate 13 arranged vertically on one side of the vertical plate 6 close to the sample area 102 and a second reset assembly 14 for resetting the first driving member 2, the second reset assembly 14 comprises:

[0078] A second blocking piece 1401 arranged on the first sliding block 10 and located on the vertical side wall of the first sliding block 10 close to the side plate 13;

[0079] A second sensing member 1402 arranged on the side plate 13 and used for detecting the second blocking piece 1401.

[0080] Specifically, the processor is in communication with the second sensing member 1402 and the first driving member 2, the second sensing member 1402 can be an optoelectronic sensing member and is in communication with the first driving member 2, when the first sliding block 10 moves to the position, the second blocking piece 1401 extends into the second detection groove of the second sensing member 1402, the potential of the second sensing member 1402 changes and sends the change to the processor, after the processor receives the change, it can be determined that the second sensing member 1402 detects the second blocking piece 1401, and then the processor controls the first driving member 2 to reset, the above arrangement ensures the accuracy of the movement of the second driving member 4 and the magnetic rod sleeve assembly 3, and further ensures the accuracy of the extraction device when performing the extraction operation.

[0081] Another embodiment of the present application provides a nucleic acid extraction device, which comprises the extraction device for the nucleic acid extraction device in the above-mentioned embodiments.

[0082] In the description of the application, it should be understood that the terms "first", "second" are used only for descriptive purposes and are not to be construed as indicating or implying relative importance or an indicated number of technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the application, the meaning of "a plurality of" is at least two, for example, two, three, etc., unless otherwise explicitly specified and limited.

[0083] In this application, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "fixing" and other terms should be understood in a broad sense, for example, can be fixedly connected, or can be detachably connected, or integrated; can be mechanically connected, or electrically connected or can communicate with each other; can be directly connected, or indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in this application can be understood according to the specific circumstances.

[0084] In the description of the specification, the description of the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the application. In this specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine the different embodiments or examples described in the specification and the features of the different embodiments or examples without contradiction.

[0085] Although the embodiments of the application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be construed as limiting the application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the application.

Claims

1. An extraction device for a nucleic acid extraction apparatus, characterized by, The extraction device comprises: a bottom plate (1) having installation areas (101) and sample areas (102) arranged side by side along the width direction; a first driving member (2) arranged in the installation areas (101) and extending along the length direction of the bottom plate (1); a magnetic rod sleeve assembly (3) arranged above the sample areas (102) and vertically movable, the magnetic rod sleeve assembly (3) being drivingly connected with the first driving member (2); a second driving member (4) arranged above the first driving member (2), the second driving member (4) extending along the length direction of the bottom plate (1) and overlapping the first driving member (2) along the width direction of the bottom plate (1); a magnetic rod (5) arranged above the sample areas (102) and vertically movable, the magnetic rod (5) being drivingly connected with the second driving member (4) and capable of being partially inserted into the magnetic rod sleeve assembly (3) or separated from the magnetic rod sleeve assembly (3) under the driving action of the second driving member (4).

2. The extraction device for a nucleic acid extraction apparatus according to claim 1, characterized by, The extraction device further comprises: a vertical plate (6) arranged above the bottom plate (1) along the vertical direction, the first driving member (2) being arranged at the bottom end of the vertical plate (6) and the second driving member (4) being arranged at the top end of the vertical plate (6); a first synchronous belt assembly (7) arranged on one side of the vertical plate (6) along the vertical direction and drivingly connected with the first driving member (2), a first synchronous belt (702) of the first synchronous belt assembly (7) being connected with the magnetic rod sleeve assembly (3); a second synchronous belt assembly (8) arranged on the side of the vertical plate (6) away from the first synchronous belt assembly (7) along the vertical direction and drivingly connected with the second driving member (4), a second synchronous belt (802) of the second synchronous belt assembly (8) being connected with the magnetic rod (5).

3. The extraction device for a nucleic acid extraction apparatus according to claim 2, characterized by, An installation space (601) for installing a tensioning mechanism is formed on the vertical plate (6) and located between the first driving member (2) and the second driving member (4).

4. The extraction device for a nucleic acid extraction apparatus according to claim 2, wherein The extraction device further comprises: a first guide rail (9) arranged on the side wall of the vertical plate (6) close to the sample areas (102) along the vertical direction; a first sliding block (10) movably arranged on the first guide rail (9), the first sliding block (10) having a first connecting portion (1001) connected with the first synchronous belt (702) and a first mounting portion (1002) for mounting the magnetic rod sleeve assembly (3); a second sliding block (11) movably arranged on the first guide rail (9) and located above the first sliding block (10), the second sliding block (11) having a second connecting portion (1101) connected with the second synchronous belt (802) and a second mounting portion (1102) for mounting the magnetic rod (5).

5. The extraction device for a nucleic acid extraction apparatus according to claim 4, characterized by, The magnetic rod sleeve assembly (3) comprises: A magnetic rod sleeve mounting piece (301) is arranged on the first mounting portion (1002), and a insertion hole (302) for the magnetic rod (5) to pass through is formed on the magnetic rod sleeve mounting piece (301); A magnetic rod sleeve (303) is arranged in the reagent tube and is detachably connected with the magnetic rod sleeve mounting piece (301).

6. The extraction device for a nucleic acid extraction apparatus according to claim 5, wherein The insertion hole (302) is funnel-shaped.

7. The extraction device for a nucleic acid extraction apparatus according to claim 4, wherein A guide portion (501) is formed on the end of the magnetic rod (5) away from the second mounting portion (1102).

8. The extraction device for a nucleic acid extraction apparatus according to claim 4, wherein The extraction device further comprises a first reset assembly (12) for resetting the second driving member (4), and the first reset assembly (12) comprises: A first blocking piece (1201) is arranged on the second sliding block (11); A first sensing member (1202) is arranged on the vertical plate (6) and is located on the same side of the second synchronous belt assembly (8) and is used for detecting the first blocking piece (1201).

9. The extraction device for a nucleic acid extraction apparatus according to claim 8, characterized by, The extraction device further comprises a side plate (13) arranged on the vertical plate (6) near the side of the sample area (102) and a second reset assembly (14) for resetting the first driving member (2), and the second reset assembly (14) comprises: A second blocking piece (1401) is arranged on the first sliding block (10) and is located on the vertical side wall of the first sliding block (10) near the side plate (13); A second sensing member (1402) is arranged on the side plate (13) and is used for detecting the second blocking piece (1401).

10. A nucleic acid extraction device, characterized by, The nucleic acid extraction device comprises the extraction device for nucleic acid extraction device according to any one of claims 1-9.

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