Anaerobic incubator
By combining components such as magnetically locked cabinet doors, vacuum pumps, heating rods, and gas tanks, the problems of sealing, temperature control, and gas replacement in traditional anaerobic culture devices are solved, thereby improving the stability of anaerobic microbial culture and the accuracy of experimental results.
Patent Information
- Application Number
- CN202423138270.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-19
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2034-12-19
AI Technical Summary
Traditional anaerobic culture devices suffer from poor sealing, inaccurate temperature control, and insufficient gas replacement. They are also cumbersome to operate and have poor observation capabilities, which affect the stability of anaerobic microbial culture and the accuracy of experimental results.
The system employs a magnetic locking cabinet door, vacuum pump, heating rod, gas tank, and sensor system to ensure the airtightness of the anaerobic environment and the accuracy of temperature control. It achieves efficient gas replacement through the vacuum pump and gas tank, and improves operational convenience and observation through transparent glass and drawer boxes.
It achieves stability and precise temperature control of the anaerobic environment, improves the success rate of anaerobic microbial cultivation and the accuracy of experimental results, simplifies the operation process and enhances the convenience of observation.
Smart Images
Figure CN223688336U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to incubator technical field, concretely is an anaerobic incubator. BACKGROUND
[0002] With the continuous development of science and technology, in the field of microbiology research, medical examination, biological pharmacy, the demand for anaerobic microorganism culture is increasing. Anaerobic microorganism is a kind of microorganism that grows and reproduces in the anaerobic environment, and they are extremely sensitive to oxygen, and cannot grow normally in the aerobic environment and even die. Therefore, in order to meet the research and application needs of anaerobic microorganism, a special anaerobic incubator is needed to create and maintain a strict anaerobic environment.
[0003] The traditional anaerobic culture device has some shortcomings. On the one hand, the sealing performance of part of the incubator is not good, which is easy to cause the infiltration of external oxygen, and affects the stability of the anaerobic environment. This may cause the cultured anaerobic microorganism to be disturbed by oxygen, and the growth is inhibited, thereby affecting the accuracy of the experimental results. On the other hand, some incubators are not accurate in temperature control, gas regulation and other aspects, and it is difficult to meet the strict requirements of different types of anaerobic microorganisms on the culture conditions. For example, some anaerobic bacteria are very sensitive to temperature changes, and small temperature fluctuations may affect their growth rate and metabolic activity. At the same time, in the gas replacement process, if the oxygen cannot be effectively removed and the required inert gas or mixed gas cannot be accurately filled, the quality of the anaerobic environment will also decrease.
[0004] In addition, the traditional anaerobic incubator also needs to be improved in terms of operation convenience and observability. For example, the opening and closing of the door of some incubators is relatively cumbersome, which is not conducive to the rapid taking and placing operation of the sample. Moreover, during the culture process, the operator often needs to observe the growth of the culture, and if the observation window of the incubator is not clear enough or the observation angle is limited, it will bring inconvenience to the experiment.
[0005] In summary, in order to overcome the defects of the existing anaerobic culture device and meet the increasing demand for anaerobic microorganism culture, it is of great practical significance to develop an anaerobic incubator with good sealing performance, precise temperature control, efficient gas replacement, and convenient operation and strong observability. UTILITY MODEL CONTENT
[0006] (I) Technical problem solved
[0007] In view of the shortcomings of the prior art, the utility model provides an anaerobic incubator, which solves the problems raised in the background art.
[0008] (II) Technical scheme
[0009] The utility model discloses in order to realize above-mentioned purpose specifically adopts following technical scheme:
[0010] An anaerobic incubator, comprising a cabinet, the side of the cabinet is rotationally hinged with a cabinet door, the bottom of the cabinet is fixedly connected with self-locking universal wheels, the inside of the cabinet door is inlaidly installed with transparent glass, the cabinet door is installed with protruding magnet blocks, the inner wall of the cabinet is inlaidly installed with recessed electromagnets, the cabinet door is fixedly installed with a handle, the inside of the cabinet forms a culture cavity, a vacuum pump is installed in the culture cavity, a heating rod is installed on the inner wall of the culture cavity, a partition plate and a drawer box capable of placing cultures are fixedly connected to the inner wall of the culture cavity, mounting plates are fixedly connected to the side walls of the cabinet, two gas tanks are placed on the mounting plates, the gas tanks are connected to the inside of the culture cavity through detachable gas pipes, and an electromagnetic switch valve is arranged at the connection between the gas pipe and the culture cavity in the cabinet.
[0011] A processor is installed in the cabinet, the processor is electrically connected with an oxygen sensor and a temperature sensor, the processor is electrically connected with an electronic display, and the processor is electrically connected with a control electromagnetic switch valve and a vacuum pump.
[0012] Further, the number of self-locking universal wheels is four, and the four self-locking universal wheels are symmetrically arranged at the four corners of the bottom of the cabinet.
[0013] Further, the electromagnets and the magnet blocks are matched in shape and size, are magnetically attracted, and are used for locking and opening the cabinet door and the cabinet.
[0014] Further, the vacuum pump sucks the gas in the culture cavity and exhausts the gas from the rear of the cabinet.
[0015] Further, the gas tank itself has a switch valve.
[0016] Further, the oxygen sensor and the temperature sensor are uniformly distributed at different positions of the inner wall of the culture cavity, so that the culture environment can be accurately monitored.
[0017] (Three) beneficial effects
[0018] Compared with the prior art, the anaerobic incubator has the following beneficial effects:
[0019] The good sealing property of the incubator is due to the magnetic attraction locking of the cabinet door and the precise machining parts, a stable anaerobic environment is created for anaerobic bacteria, the culture success rate is improved, the precise temperature control is realized by the cooperation of the heating rod and the sensor, the needs of different anaerobic bacteria are met, the efficient gas replacement is realized by the cooperation of the vacuum pump, the gas tank and the valve, the gas composition is optimized, the operation is convenient, the cabinet door is simple to open and close, the drawer type sample management is simple and flexible, the strong observation property is realized by the transparent glass, real-time monitoring is facilitated, and the anaerobic bacteria research and application development are promoted in all directions. BRIEF DESCRIPTION OF DRAWINGS
[0020] Figure 1 It is a three-dimensional structure schematic view of the utility model;
[0021] Figure 2 It is a side view structure schematic view of the utility model;
[0022] Figure 3 It is a system control schematic view of the utility model.
[0023] In the figure: 1, box; 2, cabinet door; 3, self-locking universal wheel; 4, transparent glass; 5, magnet block; 6, electromagnet; 7, handle; 8, culture chamber; 9, vacuum pump; 10, heating rod; 11, drawer box; 12, partition; 13, mounting plate; 14, gas tank; 15, gas pipe. DETAILED DESCRIPTION
[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.
[0025] EMBODIMENT
[0026] As Figures 1-3 shown, an anaerobic incubator according to one embodiment of the utility model includes a box 1;
[0027] The box 1 is cuboid in shape, and the side edges are rotatably connected to the cabinet door 2. Four self-locking universal wheels 3 are installed at the bottom corners of the box 1.
[0028] Function: Provide support structure and protective shell for the overall device, ensure normal operation of internal components, and facilitate equipment placement and transfer.
[0029] The cabinet door 2 is connected to the box 1 via a hinge, and is inlaid with transparent glass 4, a protruding magnet block 5, and a handle 7.
[0030] Function: The transparent glass 4 is used for observing samples, the magnet block 5 cooperates with the electromagnet 6 to lock the cabinet door 2 against oxygen, and the handle 7 facilitates opening and closing of the cabinet door 2 for sample placement and removal.
[0031] The self-locking universal wheels 3 are conventional universal wheels with a self-locking assembly, and are symmetrically arranged at the bottom corners of the box 1.
[0032] Function: Realize flexible movement and positioning locking of the incubator, and facilitate laboratory layout adjustment and equipment stability.
[0033] The transparent glass 4 is inlaid in the cabinet door 2, and has good sealing.
[0034] Function: To clearly observe the conditions in the culture chamber 8 without destroying the anaerobic environment, and to assist in monitoring the experimental progress.
[0035] The magnet block 5 protrudes from the frame of the cabinet door 2, and the electromagnet 6 is recessed in the inner wall of the box 1. The circuit is controlled by the processor.
[0036] Function: Form a magnetic locking mechanism to tightly close the cabinet door 2 and the box 1, maintain the anaerobic environment seal, and resist internal and external interference.
[0037] The handle 7 is fixed to the cabinet door 2.
[0038] Function: To provide operating personnel with the force to open the cabinet door 2, improve sample operation efficiency and convenience.
[0039] The culture chamber 8 is located in the core area of the box 1, with four walls closed, containing various functional components and culture samples.
[0040] Function: To create a dedicated stable space for anaerobic culture, isolate external adverse effects, and ensure constant microbial growth conditions.
[0041] The vacuum pump 9 is installed in the culture chamber 8, with an air inlet and an exhaust port through the box 1.
[0042] Function: To remove air and impurities in the chamber, create a low-oxygen or oxygen-free environment, and ensure suitable growth conditions for anaerobic bacteria.
[0043] The heating rod 10 is installed on the inner wall of the culture chamber 8 and is electrically connected to the processor.
[0044] Function: To accurately control the temperature of the chamber, meet the temperature requirements of different anaerobic bacteria growth, and maintain a stable thermal environment.
[0045] The drawer box 11 is placed in the culture chamber 8, separated by a partition 12, and can be pulled out to place samples.
[0046] Function: To store culture samples in an orderly manner, with a layered design for easy classification and management, and a drawer design for convenient operation and prevention of sample confusion and contamination.
[0047] The partition 12 is fixed to the inner wall of the culture chamber 8, dividing the space of the drawer box 11.
[0048] Function: To reasonably plan the layout of the drawer box 11, improve sample storage capacity and management efficiency, and prevent samples from interfering with each other.
[0049] The mounting plate 13 is fixed to the side wall of the box 1, carrying two gas tanks 14.
[0050] Function: To stably support the gas tank 14, ensure the reliability of the gas supply system, and facilitate installation, maintenance and replacement.
[0051] Two gas tanks 14 are placed on the mounting plate 13, with a switch valve, and are connected to the culture chamber 8 through the gas pipe 15.
[0052] Function: Store specific gases such as nitrogen and carbon dioxide, and accurately supply them to replace the gas in the chamber as needed, which is crucial for creating an anaerobic environment.
[0053] The gas pipe 15 is detachably connected to the gas tank 14 and the culture chamber 8, and an electromagnetic switch valve is provided at the connection between the box 1.
[0054] Function: Transport the gas in the gas tank 14 to the chamber, and the electromagnetic switch valve accurately controls the gas flow and the timing of the on-off, which cooperates to maintain the anaerobic condition.
[0055] The inside of the box 1 is provided with a processor, which is electrically connected to an oxygen sensor and a temperature sensor, and the processor is electrically connected to an electronic display, and the processor is electrically connected to control the electromagnetic switch valve and the vacuum pump 9.
[0056] When the anaerobic incubator is working, first open the cabinet door 2 through the handle 7 on the cabinet door 2, place the culture in the drawer box 11 in the culture chamber 8, and after closing the cabinet door 2, the electromagnet 6 is energized and the magnet block 5 is tightly magnetically attracted to lock the cabinet door 2, ensuring the sealing of the culture chamber 8. Start the vacuum pump 9, which quickly inhales the gas in the culture chamber 8 and discharges it through the rear exhaust port, and repeat the air pumping operation several times to greatly reduce the oxygen content in the chamber. Then, open the electromagnetic switch valve of the gas tank 14 and the electromagnetic switch valve of the gas pipe 15, and according to the demand, fill the specific gas such as nitrogen, carbon dioxide, etc. from the gas tank 14 on the mounting plate 13 into the culture chamber 8 through the gas pipe 15, realize gas replacement, and build an oxygen-free or low-oxygen environment. At the same time, the heating rod 10 is accurately adjusted according to the processor settings to adjust the temperature in the culture chamber 8 to the appropriate range for anaerobic bacteria growth, and the oxygen sensor and temperature sensor are evenly distributed on the inner wall of the culture chamber 8, which monitors the environmental parameters in real time and feeds back to the processor, which adjusts the operation of each component accordingly. The data is directly presented on the electronic display, and the whole process ensures that the anaerobic bacteria grow and reproduce in a stable and accurately controlled environment.
[0057] As shown in Figure 1 In some embodiments, the self-locking universal wheel 3 has four, symmetrically placed at the bottom corners of the box 1; it is convenient to move the incubator in different areas of the laboratory, easily adjust the position, and meet the needs of experimental layout changes. If the laboratory space is limited and the equipment needs to be temporarily moved, or the incubator placement point needs to be changed according to the experimental process optimization, the universal wheel can quickly achieve it, reducing the cost of manual labor and the risk of equipment damage.
[0058] As shown in Figure 1As shown in the drawings, in some embodiments, the electromagnet 6 and the magnet block 5 are matched in size and shape, and are magnetically attracted to each other to lock the cabinet door 2 and the cabinet 1; this magnetic attraction locking method is simple to operate, and only needs to control the on-off circuit of the electromagnet 6 to realize the opening and closing action of the cabinet door 2, without the need for complex mechanical lock insertion or screwing operation, thereby reducing the labor intensity and operation time of the operator, and improving the efficiency of sample taking and placing and equipment maintenance. Moreover, the magnetic attraction force is uniform and stable, which avoids deformation of the cabinet door 2 or poor local sealing, thereby prolonging the service life and maintenance period of the equipment.
[0059] As shown in the drawings, Figure 2 As shown in the drawings, in some embodiments, the vacuum pump 9 sucks the gas in the culture chamber 8 and discharges it from the rear of the cabinet 1; during the anaerobic culture preparation stage, the vacuum pump 9 is operated at high speed to rapidly remove the original air in the culture chamber 8, including a large amount of oxygen and other trace impurity gases. After multiple cycles of air pumping, the oxygen concentration in the chamber is reduced to a very low level, which lays a key foundation for subsequent inert gas filling to create an oxygen-free or strictly low-oxygen environment, ensures that anaerobic bacteria are not harmed by oxygen toxicity, and normally starts the growth and metabolism program, thereby improving the culture success rate and experimental accuracy.
[0060] As shown in the drawings, Figure 2 As shown in the drawings, in some embodiments, the gas tank 14 itself has a switch valve; the switch valve can be completely closed during the non-use period of the gas tank 14 or during equipment maintenance, effectively preventing the accidental leakage of high-pressure gas in the tank from causing safety hazards, and protecting laboratory personnel and the environment. At the same time, if a certain gas tank 14 needs to be replaced or repaired, only the switch valve of the tank needs to be closed to isolate the tank, without affecting the supply of other gases and the overall operation of the incubator, thereby simplifying the maintenance operation process, reducing equipment downtime, and ensuring continuous and stable progress of experiments and efficient and reliable operation of the equipment.
[0061] As shown in the drawings, Figure 3 As shown in the drawings, in some embodiments, the oxygen sensor and the temperature sensor are uniformly distributed at different positions on the inner wall of the culture chamber 8 to ensure accurate monitoring of the culture environment; a large amount of sensor data accumulated during long-term culture experiments is analyzed and processed to provide key basis for optimizing the culture scheme. Based on data insight into the dynamic changes in oxygen and temperature requirements of different anaerobic bacteria at different growth stages, researchers can accurately adjust the gas filling strategy, temperature set value, and equipment operation parameters, thereby improving the adaptability and universality of the incubator, accelerating the scientific research process, enhancing the quality of research results, and promoting the development and innovation in the field of anaerobic bacteria.
[0062] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application have been described in detail, for the skilled in the art, it still can be modified, or for the equivalent replacement of part of the technical features of the technical solutions recorded in the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, shall be included within the scope of the present application.
Claims
1. An anaerobic incubator comprising a cabinet (1), characterised in that: The side of the box (1) is rotatably connected with a cabinet door (2), the bottom of the box (1) is fixedly connected with a self-locking universal wheel (3), the inside of the cabinet door (2) is inlaidly installed with a transparent glass (4), the cabinet door (2) is installed with a protruding magnet block (5), the inner wall of the box (1) is inlaidly installed with a recessed electromagnet (6), the cabinet door (2) is fixedly installed with a handle (7), the inside of the box (1) forms a culture cavity (8), the culture cavity (8) is installed with a vacuum pump (9), the inner wall of the culture cavity (8) is installed with a heating rod (10), the inner wall of the culture cavity (8) is fixedly connected with a partition plate (12) and a drawer box (11) capable of placing culture, the side wall of the box (1) is fixedly connected with a mounting plate (13), the mounting plate (13) is placed with two gas tanks (14), the gas tank (14) is connected to the inside of the culture cavity (8) through a detachable gas pipe (15), wherein the gas pipe (15) to the culture cavity (8) is provided with an electromagnetic switch valve at the connection of the box (1). The inside of the box (1) is installed with a processor, the processor is electrically connected with an oxygen sensor and a temperature sensor, the processor is electrically connected with an electronic display, the processor is electrically connected with a control electromagnetic switch valve and a vacuum pump (9).
2. An anaerobic incubator according to claim 1, wherein: The number of the self-locking universal wheel (3) is four, which is symmetrically arranged at the four corners of the bottom of the box (1).
3. An anaerobic incubator according to claim 1, wherein: The electromagnet (6) and the magnet block (5) are matched in size and shape, and are magnetically attracted to lock and open the cabinet door (2) and the box (1).
4. An anaerobic incubator according to claim 1, wherein: The vacuum pump (9) sucks the gas in the culture cavity (8) and exhausts from the rear of the box (1).
5. An anaerobic incubator according to claim 1, wherein: The gas tank (14) itself has a switch valve.
6. An anaerobic incubator according to claim 1, wherein: The oxygen sensor and the temperature sensor are uniformly distributed at different positions of the inner wall of the culture cavity (8) to ensure accurate monitoring of the culture environment.