Immune cell culture bag set convenient for changing liquid
By designing an immune cell culture bag kit that facilitates medium changes, and utilizing the closed connection between the replenishment bag, cell culture bag, and waste bag, the cumbersome medium change operation and the risk of bacterial contamination are solved, achieving efficient and low-cost cell culture.
Patent Information
- Application Number
- CN202423100601.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-13
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2034-12-13
AI Technical Summary
In current immune cell culture processes, changing the medium is cumbersome and prone to contamination, and there is a risk of bacterial contamination when using equipment such as pipe fitting machines.
Design an immune cell culture bag kit that facilitates fluid exchange, including a replenishment bag, a cell culture bag, and a waste bag, which are connected by a sterile filter and silicone tubing to form a closed kit, reducing manual operation and lowering the risk of contamination.
It achieves a sterile culture environment, reduces manual operation costs and contamination risks, improves cell culture expansion efficiency, and avoids bacterial contamination problems caused by welded pipes.
Smart Images
Figure CN223688358U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture technical field, specifically, relate to a kind of immune cell culture bag kit convenient to change liquid. BACKGROUND
[0002] Cellular immunotherapy is a rapidly developing technology in the field of oncology, which is the fourth treatment technology after surgery, radiotherapy and chemotherapy. Cellular immunotherapy collects autologous peripheral blood from patients, isolates immune cells, and expands them in vitro through induction and culture. Finally, it is a treatment method that kills tumor cells by returning to the patient's body.
[0003] Currently, immune cells are generally expanded in high density using cell culture bags. Cell culture bags are made of special materials and are air-permeable but water-tight. Compared to traditional cell culture bottles, they have a large gas exchange area and support high-density cell growth. During immune cell culture, it is essential to supplement fresh medium and replace old medium. High-density expansion of immune cell culture bags is usually done by liquid replacement operation in a biological safety cabinet or by connecting the pipeline using a pipe connector and a pipe sealer, and then performing liquid replacement operation. These two methods have two drawbacks: first, when performing liquid replacement operation in a biological safety cabinet, the medium in the bottle needs to be transferred to the culture bag, and there are many places for pipe connection operation, which can easily lead to contamination. Second, during the operation of the pipe connector and the pipe sealer, some smoke and odor are generated, which can contaminate the environment of the clean room and cannot completely ensure the firmness of the pipeline welding, which may cause contamination. SUMMARY
[0004] In view of the defects in the prior art, the utility model aims to provide an immune cell culture bag kit convenient for liquid replacement.
[0005] According to the immune cell culture bag kit convenient for liquid replacement provided by the utility model, the liquid supplementing bag, the cell culture bag, the waste liquid bag and the sterile filter are connected by the first pipeline, the second pipeline and the third pipeline.
[0006] The liquid inlet of the liquid supplementing bag is connected with the first pipeline.
[0007] The liquid outlet of the liquid supplementing bag is connected with the liquid supplementing port of the cell culture bag through the second pipeline.
[0008] The liquid outlet of the cell culture bag is connected with the liquid inlet of the waste liquid bag through the third pipeline.
[0009] Among them, the first pipeline is provided with a sterile filter, and the liquid outlet of the cell culture bag is provided with a filter membrane.
[0010] Preferably, the bottom of the liquid supplementing bag is provided with a first sampling port.
[0011] Preferably, the top of the cell culture bag is provided with an injection port.
[0012] Preferably, the injection port is provided with a heparin sodium cap.
[0013] Preferably, the bottom of the cell culture bag is provided with a second sampling port.
[0014] Preferably, the first sampling port of the supplement liquid bag and the second sampling port of the cell culture bag are provided with a luer needleless sampling head.
[0015] Preferably, the first pipeline, the second pipeline and the third pipeline are silica gel pipelines.
[0016] Preferably, the second pipeline and the third pipeline are provided with a clip.
[0017] Preferably, the filter membrane is bonded to the inner side end of the cell culture bag outlet.
[0018] Preferably, the sterile filter divides the first pipeline into a front end pipeline and a rear end pipeline, and the front end pipeline is provided with a clip.
[0019] Compared with the prior art, the utility model has the beneficial effects that:
[0020] The utility model discloses a pipeline is connected in proper order with supplement liquid bag, cell culture bag, waste liquid bag and forms complete airtight immune cell culture liquid change kit, provides the strict aseptic culture environment for cell, solves the multiple manual transfer fresh culture medium replacement old culture medium's shortcoming, greatly reduces the manual operation cost and the pollution risk, improves cell culture expansion efficiency, solves the risk that cell is contaminated in the complicated operation of liquid change, also solves the risk that the liquid leakage leads to the contamination when using the equipment such as pipe connection machine welds the pipeline, reduces the cost. BRIEF DESCRIPTION OF DRAWINGS
[0021] Other features, objects and advantages of the utility model will become more apparent through reading the following detailed description of non-restrictive embodiments with reference to the accompanying drawings:
[0022] Figure 1 It is the structural schematic diagram of the utility model.
[0023] The drawings show that:
[0024] Supplement liquid bag 1
[0025] First pipeline 11
[0026] First sampling port 12
[0027] Cell culture bag 2
[0028] Second pipeline 21
[0029] injection port 22
[0030] filter membrane 23
[0031] second sampling port 24
[0032] waste liquid bag 3
[0033] sterile filter 4 DETAILED DESCRIPTION
[0034] The utility model will be described in detail below in combination with specific embodiments. The following embodiments will help the person skilled in the art to further understand the utility model, but do not limit the utility model in any form. It should be pointed out that for those skilled in the art, without departing from the concept of the utility model, a number of changes and improvements can be made. These all belong to the protection scope of the utility model.
[0035] The utility model provides a kind of immune cell culture bag set of liquid exchange, as shown in figure 1, including liquid supplement bag 1, cell culture bag 2, waste liquid bag 3 and sterile filter 4, the liquid inlet of liquid supplement bag 1 is connected with first pipeline 11, first pipeline 11 is liquid inlet pipeline, to reduce bacterial infection, first pipeline 11 is provided with sterile filter 4, sterile filter 4 divides first pipeline 11 into front end pipe and rear end pipe, front end pipe is equipped with clip, the on-off of first pipeline 11 can be controlled by being set clip, whether liquid supplement bag 1 is filled can be realized. Figure 1 As shown in figure 2, the liquid outlet of liquid supplement bag 1 is connected with the feeding port of cell culture bag 2 by second pipeline 21, second pipeline 21 is also equipped with clip, the on-off of second pipeline 21 is controlled by clip, whether to transport sterile culture medium to cell culture bag 2 is realized, the liquid inlet of waste liquid bag 3 is connected with the liquid outlet of cell culture bag 2 by third pipeline 31, third pipeline 31 is equipped with clip, and the on-off of third pipeline 31 is controlled by the clip.
[0036] Figure 1 As shown in figure 2, the liquid outlet of liquid supplement bag 1 is connected with the feeding port of cell culture bag 2 by second pipeline 21, second pipeline 21 is also equipped with clip, the on-off of second pipeline 21 is controlled by clip, whether to transport sterile culture medium to cell culture bag 2 is realized, the liquid inlet of waste liquid bag 3 is connected with the liquid outlet of cell culture bag 2 by third pipeline 31, third pipeline 31 is equipped with clip, and the on-off of third pipeline 31 is controlled by the clip.
[0037] The utility model sterile filter 4, liquid supplement bag 1, cell culture bag 2 and waste liquid bag 3 form complete closed type kit, reduce the operation of manual liquid transfer, reduce the pollution risk.
[0038] The first pipeline 11, second pipeline 21 and third pipeline 31 in the utility model are preferably silica gel pipeline.
[0039] In order to block cell flow into waste liquid bag 3, filter membrane 23 is arranged on the liquid outlet of cell culture bag 2, reduces manual operation cost, improves the overall cell culture amplification efficiency. Filter membrane 23 is preferably bonded on the inside end of the liquid outlet of cell culture bag 2.
[0040] Specifically, the bottom of the supplement liquid bag 1 is provided with a first sampling port 12 for measuring biochemical indexes of the culture medium.
[0041] Specifically, the bottom of the cell culture bag 2 is provided with a second sampling port 24 for sampling and detecting the liquid in the cell culture bag 2, and the top of the cell culture bag 2 is provided with an injection port 22 through which the required medicament can be injected into the cell culture bag 2.
[0042] In order to reduce pollution, the injection port 22 is provided with a heparin sodium cap, and in order to facilitate operation, the first sampling port 12 and the second sampling port 24 are both provided with a luer needle-free sampling head.
[0043] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.
[0044] The specific embodiments of the present application are described above. It should be understood that the present application is not limited to the specific embodiments described above, and those skilled in the art can make various changes or modifications within the scope of the claims, which does not affect the essential content of the present application. In the case of no conflict, the embodiments of the present application and the features in the embodiments can be combined with each other arbitrarily.
Claims
1. An immune cell culture bag set facilitating liquid exchange, characterized by, The application relates to a cell culture device, which comprises a liquid supplementing bag (1), a cell culture bag (2), a waste liquid bag (3) and a sterile filter (4). A first pipeline (11) is connected to the liquid inlet of the liquid supplementing bag (1); The liquid outlet of the liquid supplementing bag (1) is connected to the liquid supplementing inlet of the cell culture bag (2) through a second pipeline (21); The liquid outlet of the cell culture bag (2) is connected to the liquid inlet of the waste liquid bag (3) through a third pipeline (31); The first pipeline (11) is provided with the sterile filter (4), and the liquid outlet of the cell culture bag (2) is provided with a filter membrane (23).
2. The immune cell culture bag set with easy liquid exchange according to claim 1, characterized in that, The bottom of the liquid supplementing bag (1) is provided with a first sampling port (12).
3. The immune cell culture bag set with easy liquid exchange of claim 1, wherein, The top of the cell culture bag (2) is provided with an injection port (22).
4. The immune cell culture bag set with easy liquid exchange according to claim 3, characterized in that, The injection port (22) is provided with a heparin sodium cap.
5. The immune cell culture bag set with easy liquid exchange of claim 1, wherein, The bottom of the cell culture bag (2) is provided with a second sampling port (24).
6. The immune cell culture bag set with easy liquid exchange of claim 1, wherein, The first sampling port (12) of the liquid supplementing bag (1) and the second sampling port (24) of the cell culture bag (2) are both provided with a luer needleless sampling head.
7. The immune cell culture bag set with easy liquid exchange of claim 1, wherein, The first pipeline (11), the second pipeline (21) and the third pipeline (31) are all silica gel pipelines.
8. The immune cell culture bag set with easy liquid exchange of claim 1, wherein, The second pipeline (21) and the third pipeline (31) are both provided with a clip.
9. The immune cell culture bag set with easy liquid exchange of claim 1, wherein, The filter membrane (23) is adhered to the inner side end of the liquid outlet of the cell culture bag (2).
10. The immune cell culture bag set with easy liquid exchange of claim 1, wherein, The sterile filter (4) divides the first pipeline (11) into a front-end pipeline and a rear-end pipeline, and the front-end pipeline is provided with a clip.