High-magnification high-resolution microscopic optical system
By using an infinite conjugate optical structure and lens combination, the problems of aperture aberration and field aberration in microscopic optical systems have been solved, enabling high-magnification, high-resolution microscopic imaging and improving imaging clarity and contrast.
Patent Information
- Application Number
- CN202520266113.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-19
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2035-02-19
AI Technical Summary
Traditional high-magnification, high-resolution imaging microscopy optical systems suffer from aperture aberration, field aberration, and stray light effects when observing microscopic biological tissues, resulting in decreased image clarity and contrast.
By employing an infinite conjugate optical structure, astigmatism and field curvature are balanced by introducing a meniscus negative power lens L3, and aperture aberration is corrected by introducing meniscus positive power lenses L4 and L5. Furthermore, stray light is suppressed by using a field stop S1 and an aperture stop S2, thereby achieving secondary imaging.
It achieves flat-field image quality under high magnification, improves imaging clarity and contrast, reaches diffraction-limited imaging quality, and has a compact structure.
Smart Images
Figure CN223692575U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to optical technology field, concretely relates to a big magnification high resolution microscopic optical system. BACKGROUND
[0002] The microscopic optical system is used for observing micro biological tissue, and the micro biological tissue is irradiated by laser, and then the imaging of the micro biological tissue is realized by using scattered light or weak fluorescence, but the traditional big magnification and high resolution imaging usually brings aperture aberration (including spherical aberration, coma and astigmatism etc.), field aberration (including distortion, field curvature and chromatic aberration etc.), and these aberrations can cause the decline of the imaging definition and the image distortion. SUMMARY
[0003] Therefore, the utility model provides a big magnification high resolution microscopic optical system, which can correct spherical aberration and field curvature and other aberrations, effectively suppress stray light, and is suitable for high contrast microscopic imaging of micro biological tissue under the condition of weak scattered light or fluorescence.
[0004] To achieve the above object, the utility model provides the following technical scheme.
[0005] A big magnification high resolution microscopic optical system comprises:
[0006] A biconvex positive focal length lens L1, the curvature radius of the front surface is 21.972mm, the light transmission aperture is 2.90mm, the curvature radius of the rear surface is 23.502mm, the light transmission aperture is 2.79mm, and the focal length is set as phi L1;
[0007] A field diaphragm S1, the light transmission aperture is 1.25mm;
[0008] A biconvex positive focal length lens L2, the curvature radius of the front surface is 84.252mm, the light transmission aperture is 16.42mm, the curvature radius of the rear surface is 41.424mm, the light transmission aperture is 16.42mm, and the focal length is set as phi L2;
[0009] A meniscus negative focal length thick lens L3, the curvature radius of the front surface is -16.131mm, the light transmission aperture is 16.24mm, the curvature radius of the rear surface is -26.619mm, the light transmission aperture is 22.43mm, and the focal length is set as phi L3;
[0010] An aperture diaphragm S2, the light transmission aperture is 21.81mm;
[0011] a meniscus positive power lens L4, a radius of curvature of a front surface of which is 20.003 mm, an aperture of which is 21.22 mm, a radius of curvature of a back surface of which is 61.956 mm, an aperture of which is 19.22 mm, and a power of which is set as φL4;
[0012] a meniscus positive power lens L5, a radius of curvature of a front surface of which is 7.695 mm, an aperture of which is 14.71 mm, a radius of curvature of a back surface of which is 7.672 mm, an aperture of which is 10.85 mm, and a power of which is set as φL5;
[0013] The power of each lens and the power φ of the microscopic optical system satisfy the following relationships respectively:
[0014] 0.06≤φL1 / φ≤0.12;
[0015] 0.02≤φL2 / φ≤0.05;
[0016] -0.005≤φL3 / φ≤-0.002;
[0017] 0.03≤φL4 / φ≤0.06;
[0018] 0.035≤φL5 / φ≤0.065;
[0019] The distance L from the edge of the meniscus positive power lens L5 to the object plane and the focal length f of the microscopic optical system satisfy: L≥3.5f.
[0020] By adopting an infinite conjugate optical structure, imaging is realized in a way of secondary imaging, by introducing the meniscus negative power thick lens L3, chromatic aberration and field curvature under the full field of view are balanced, flat field image quality is obtained under high magnification imaging, by introducing the meniscus positive power lens L4 and the meniscus positive power lens L5, an un-vignetting lens is formed to correct aperture aberration caused under a large numerical aperture, by introducing the field stop S1 and the aperture stop S2, stray light is effectively suppressed, and thus clear high magnification and high resolution imaging is obtained.
[0021] The interval of the biconvex positive power lens L1 and the field stop S1 on the central axis is 11.22 mm;
[0022] The interval of the field stop S1 and the biconvex positive power lens L2 on the central axis is 124.12 mm;
[0023] The interval of the biconvex positive power lens L2 and the meniscus negative power thick lens L3 on the central axis is 1.85 mm;
[0024] The interval of the meniscus negative power thick lens L3 and the aperture stop S2 on the central axis is 0.38 mm.
[0025] The interval of the aperture diaphragm S2 and the meniscus positive power lens L4 on the central axis is 1.68mm;
[0026] The interval of the meniscus positive power lens L4 and the meniscus positive power lens L5 on the central axis is 0.1mm;
[0027] The interval of the meniscus positive power lens L5 and the image plane on the central axis is 6.05mm;
[0028] The thickness of the biconvex positive power lens L1 on the central axis is 4mm;
[0029] The thickness of the biconvex positive power lens L2 on the central axis is 5.22mm;
[0030] The thickness of the meniscus negative power thick lens L3 on the central axis is 19.92mm;
[0031] The thickness of the meniscus positive power lens L4 on the central axis is 5.26mm;
[0032] The thickness of the meniscus positive power lens L5 on the central axis is 4.89mm.
[0033] The material of the biconvex positive power lens L1 is H-ZF88, and the materials of the biconvex positive power lens L2, the meniscus negative power thick lens L3, the meniscus positive power lens L4 and the meniscus positive power lens L5 are all H-ZF52. BRIEF DESCRIPTION OF DRAWINGS
[0034] Figure 1 It is the component structure schematic view of the micro optical system of the utility model.
[0035] Figure 2 It is the wave aberration diagram of the micro optical system of the utility model. DETAILED DESCRIPTION
[0036] The application will be described in detail below in combination with specific embodiments.
[0037] As Figure 1 shown, a high magnification and high resolution micro optical system, from the object plane to the image plane along the optical axis, in sequence from front to back, includes: a biconvex positive power lens L1, a field diaphragm S1, a biconvex positive power lens L2, a meniscus negative power thick lens L3, an aperture diaphragm S2, a meniscus positive power lens L4 and a meniscus positive power lens L5, wherein the focal power of the biconvex positive power lens L1 φL1 and the focal power of the micro optical system φ satisfy:
[0038] 0.06≤φL1 / φ≤0.12;
[0039] The power φL2 of the biconvex positive power lens L2 and the power φ of the microscopic optical system satisfy:
[0040] 0.02≤φL2 / φ≤0.05;
[0041] The power φL3 of the meniscus negative power thick lens L3 and the power φ of the microscopic optical system satisfy:
[0042] -0.005≤φL3 / φ≤-0.002;
[0043] The power φL4 of the meniscus positive power lens L4 and the power φ of the microscopic optical system satisfy:
[0044] 0.03≤φL4 / φ≤0.06;
[0045] The power φL5 of the meniscus positive power lens L5 and the power φ of the microscopic optical system satisfy:
[0046] 0.035≤φL5 / φ≤0.065;
[0047] The distance L from the edge of the meniscus positive power lens L5 to the object plane and the focal length f of the microscopic optical system satisfy: L≥3.5f.
[0048] By adopting an infinite conjugate optical structure, imaging is achieved by using a secondary imaging mode, by introducing the meniscus negative power thick lens L3, the astigmatism and field curvature and other aberrations under the full field of view are balanced, a flat field image quality is obtained under a large magnification imaging, by introducing the meniscus positive power lens L4 and the meniscus positive power lens L5 to form a non-halation lens, aperture aberrations caused by a large numerical aperture are corrected, by introducing the field stop S1 and the aperture stop S2, stray light is effectively suppressed and the light intensity is adjusted, so that clear large magnification and high resolution imaging is obtained.
[0049] Specifically, the curvature radius of the front surface of the biconvex positive power lens L1 is 21.972 mm, the clear aperture is 2.90 mm, the curvature radius of the rear surface is 23.502 mm, and the clear aperture is 2.79 mm. The clear aperture of the field stop S1 is 1.25 mm. Moreover, the interval of the biconvex positive power lens L1 and the field stop S1 on the central axis is 11.22 mm, and the thickness of the biconvex positive power lens L1 on the central axis is 4 mm.
[0050] The radius of curvature of the front surface of the biconvex positive power lens L2 is 84.252 mm, and the clear aperture is 16.42 mm, and the radius of curvature of the back surface is 41.424 mm, and the clear aperture is 16.42 mm. And the interval between the field stop S1 and the biconvex positive power lens L2 on the central axis is 124.12 mm, and the thickness of the biconvex positive power lens L2 on the central axis is 5.22 mm.
[0051] The radius of curvature of the front surface of the meniscus negative power thick lens L3 is -16.131 mm, and the clear aperture is 16.24 mm, and the radius of curvature of the back surface is -26.619 mm, and the clear aperture is 22.43 mm. And the interval between the biconvex positive power lens L2 and the meniscus negative power thick lens L3 on the central axis is 1.85 mm, and the thickness of the meniscus negative power thick lens L3 on the central axis is 19.92 mm.
[0052] The clear aperture of the aperture stop S2 is 21.81 mm. And the interval between the meniscus negative power thick lens L3 and the aperture stop S2 on the central axis is 0.38 mm.
[0053] The radius of curvature of the front surface of the meniscus positive power lens L4 is 20.003 mm, and the clear aperture is 21.22 mm, and the radius of curvature of the back surface is 61.956 mm, and the clear aperture is 19.22 mm. And the interval between the aperture stop S2 and the meniscus positive power lens L4 on the central axis is 1.68 mm, and the thickness of the meniscus positive power lens L4 on the central axis is 5.26 mm.
[0054] The radius of curvature of the front surface of the meniscus positive power lens L5 is 7.695 mm, and the clear aperture is 14.71 mm, and the radius of curvature of the back surface is 7.672 mm, and the clear aperture is 10.85 mm. And the interval between the meniscus positive power lens L4 and the meniscus positive power lens L5 on the central axis is 0.1 mm, and the thickness of the meniscus positive power lens L5 on the central axis is 4.89 mm.
[0055] The material of the biconvex positive power lens L1 is H-ZF88, and the materials of the biconvex positive power lens L2, the meniscus negative power thick lens L3, the meniscus positive power lens L4 and the meniscus positive power lens L5 are all H-ZF52.
[0056] Wherein, the image plane can be a high-sensitivity CCD or CMOS camera target surface.
[0057] Wherein, the central axis coincides with the optical axis,
[0058] As one of the most preferred schemes, the optical parameters of the present microscopic optical system are shown in the following table.
[0059]
[0060] It is worth noting that the mirror surface represented by the surface number corresponds to the mirror surface of each lens arranged in order from the object side to the image side along the optical axis, and when the lens is illuminated with a convex surface, the radius of curvature is positive; when illuminated with a concave surface, the radius of curvature is negative.
[0061] In the present embodiment, the technical indicators achieved by the above parameter settings are as follows:
[0062] Object numerical aperture: 0.8;
[0063] Object imaging line size: 0.1 mm;
[0064] Object working distance: 3.5 mm;
[0065] System focal length: 1 mm;
[0066] Imaging wavelength: 532 nm;
[0067] Optical total length: ≤180 mm.
[0068] The microscopic optical system mainly solves the correction problem of aperture aberration and field aberration caused by realizing high magnification and high resolution when observing micro biological tissues. In order to realize high magnification imaging and obtain clear images of micro biological tissues after magnification, the focal length of the microscopic optical system is controlled to be 1 mm, and after matching with the standard barrel lens on the market, the magnification can reach 200 times. In order to see the details, the numerical aperture of the microscopic optical system reaches 0.8, and the resolution is better than 405 nm. In order to correct the aperture aberration under high numerical aperture, the meniscus positive power lens L4 and the meniscus positive power lens L5 are introduced to form a non-halation lens, and in order to balance the astigmatism and field curvature under the full field of view, the meniscus negative power thick lens L3 is introduced, and the flat field image quality is obtained under high magnification imaging.
[0069] The present microscopic optical system perfectly corrects spherical aberration, coma, astigmatism, field curvature and other aberrations, and obtains diffraction-limited imaging quality. In addition, the present microscopic optical system adopts a secondary imaging light path, and a field stop S1 for eliminating stray light is arranged at the intermediate image plane, which can only allow the light of the imaging part to pass through the microscopic optical system to reach the imaging camera, greatly reducing the background stray light, improving the imaging contrast and clarity, and being more conducive to realizing the collection and imaging of weak signals of micro biological tissues. The present microscopic optical system has an imaging resolution better than 405 nm under a working distance of not less than 3.5 mm.
[0070] Reference Figure 2The wave aberration distribution of the micro optical system in the object space is characterized, wherein the minimum root mean square wave aberration is 0.019λ, the maximum is 0.043λ, and the average is 0.025λ, reaching the diffraction limit. The total length of the micro optical system is not more than 180mm, and only 5 lenses are used to reach the diffraction limit imaging quality, which is compact in structure and high in application value.
[0071] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application, and are not intended to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.
Claims
1. A large-magnification high-resolution microscopic optical system characterized by comprising: In order from front to back along the optical axis from the object plane to the image plane, the system comprises: a biconvex positive lens L1, the front surface of which has a radius of curvature of 21.972 mm and a clear aperture of 2.90 mm, and the back surface of which has a radius of curvature of 23.502 mm and a clear aperture of 2.79 mm, and the optical power of which is set to be φL1; a field stop S1, the clear aperture of which is 1.25 mm; a biconvex positive lens L2, the front surface of which has a radius of curvature of 84.252 mm and a clear aperture of 16.42 mm, and the back surface of which has a radius of curvature of 41.424 mm and a clear aperture of 16.42 mm, and the optical power of which is set to be φL2; a meniscus negative thick lens L3, the front surface of which has a radius of curvature of -16.131 mm and a clear aperture of 16.24 mm, and the back surface of which has a radius of curvature of -26.619 mm and a clear aperture of 22.43 mm, and the optical power of which is set to be φL3; an aperture stop S2, the clear aperture of which is 21.81 mm; a meniscus positive lens L4, the front surface of which has a radius of curvature of 20.003 mm and a clear aperture of 21.22 mm, and the back surface of which has a radius of curvature of 61.956 mm and a clear aperture of 19.22 mm, and the optical power of which is set to be φL4; a meniscus positive lens L5, the front surface of which has a radius of curvature of 7.695 mm and a clear aperture of 14.71 mm, and the back surface of which has a radius of curvature of 7.672 mm and a clear aperture of 10.85 mm, and the optical power of which is set to be φL5; The optical power of each lens and the optical power φ of the microscopic optical system satisfy the following relationships respectively: 0.06≤φL1 / φ≤0.12; 0.02≤φL2 / φ≤0.05; -0.005≤φL3 / φ≤-0.002; 0.03≤φL4 / φ≤0.06; 0.035≤φL5 / φ≤0.065; The distance L from the edge of the meniscus positive lens L5 to the object plane and the focal length f of the microscopic optical system satisfy: L≥3.5f.
2. The large-magnification high-resolution microscopic optical system according to claim 1, wherein The interval of the biconvex positive lens L1 and the field stop S1 on the central axis is 11.22 mm; The interval of the field stop S1 and the biconvex positive lens L2 on the central axis is 124.12 mm; The interval of the biconvex positive lens L2 and the meniscus negative thick lens L3 on the central axis is 1.85 mm; The interval of the meniscus negative thick lens L3 and the aperture stop S2 on the central axis is 0.38 mm; The interval of the aperture stop S2 and the meniscus positive lens L4 on the central axis is 1.68 mm; The interval of the meniscus positive lens L4 and the meniscus positive lens L5 on the central axis is 0.1 mm; The interval of the meniscus positive lens L5 and the image plane on the central axis is 6.05 mm; The thickness of the biconvex positive lens L1 on the central axis is 4 mm; The thickness of the biconvex positive lens L2 on the central axis is 5.22 mm; The thickness of the meniscus negative thick lens L3 on the central axis is 19.92 mm; The thickness of the meniscus positive power lens L4 on the central axis is 5.26mm; The thickness of the meniscus positive power lens L5 on the central axis is 4.89mm.
3. The large-magnification high-resolution microscopic optical system according to claim 2, wherein The material of the lenticular positive power lens L1 is H-ZF88, and the materials of the lenticular positive power lens L2, the meniscus negative power thick lens L3, the meniscus positive power lens L4 and the meniscus positive power lens L5 are all H-ZF52.