Sample tube shading positioning structure and multi-index qPCR (quantitative polymerase chain reaction) detection device

Abstract

The utility model relates to a sample tube shading and positioning structure and a multi-index qPCR (quantitative polymerase chain reaction) detection device, the sample tube shading and positioning structure comprises a positioning table, the top of the positioning table is provided with a limiting groove, the side wall of the limiting groove is provided with a plurality of plungers protruding towards the inner side of the limiting groove; a plurality of positioning grooves are sequentially formed in the bottom wall of the limiting groove in the length direction of the limiting groove, a plurality of positioning through holes are formed in the positioning grooves respectively, and the positioning through holes are used for containing sample tubes; the plurality of shading covers are respectively arranged above the plurality of positioning grooves in a covering manner, the shading covers are used for covering the sample tubes positioned in the positioning through holes on the inner sides of the shading covers, plunger clamping grooves are formed in the outer walls of the shading covers, and the plungers are clamped in the plunger clamping grooves of the shading covers at the corresponding positions. According to the utility model, the technical problem that the accuracy of the detection result of the sample to be detected is poor is solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to molecular diagnostic technique field especially, relate to a sample tube light shielding positioning structure and multi -index detection qPCR's device. BACKGROUND

[0002] Molecular diagnostic technique refers to with DNA and RNA as diagnostic material, with molecular biology technique through the detection gene's existence, defect or expression exception, thereby to the human state and disease make the diagnosis technique. Its basic principle is to detect whether the structure of DNA or RNA changes, how much and whether the expression function is abnormal, to determine whether the abnormal change of the examinee at gene level, to disease prevention, prediction, diagnosis, treatment and prognosis have important significance. Popularly simple all the methodological techniques based on molecular biology level belong to molecular diagnostic technique, such as polymerase chain reaction technology (also called PCR technology), gene sequencing technology and so on.

[0003] PCR (Polymerase chain reaction) technology is a kind of molecular biology technology for amplifying specific DNA fragments (to be tested gene) of to-be-tested sample, that is, specific in vitro amplification of DNA fragments. The basic principle of PCR is similar to the natural replication process of DNA, which consists of three basic reaction steps of denaturation, annealing and extension: denaturation of template DNA, annealing (renaturation) of template DNA and primer, and extension of primer. Repeat the three processes of denaturation, annealing and extension, more "semi-retained replication chain" can be obtained, and this new chain can become the template for the next cycle.

[0004] Real-time fluorescent quantitative polymerase chain reaction (Quantitative Real-time Polymerase Chain Reaction, qPCR) is to add a reporter group in the PCR reaction system of to-be-tested sample for a specific DNA fragment. When a specific DNA fragment undergoes a reaction cycle (that is, after undergoing replication), the reporter group emits a fluorescence signal that is enhanced once. By detecting the change of fluorescence signal intensity after each reaction cycle, the change of reaction product quantity can be monitored in real time. According to the monitoring result, qualitative and quantitative analysis of to-be-tested sample can be carried out.

[0005] PCR instrument is a key instrument for realizing PCR technology, with the increase of market detection demand, different application scenarios, the improvement of the accuracy requirement of detection result, the existing PCR instrument cannot meet the above requirements. For example, the existing PCR instrument can only realize the simultaneous detection of batch samples, cannot realize the on-site detection of large quantities of different samples, which will cause the patient to wait for a long time for the detection result; in addition, the existing PCR instrument has poor stability for the placement position of the sample tube containing the sample, which causes the sample tube installed in place to be unable to quickly transfer heat, and affects the accuracy of sample detection; and the existing PCR instrument also has the problem of poor light shielding effect for the placement position of the sample tube containing the sample, which causes the sample in the sample tube to be easily affected by the external environment, which also affects the accuracy of sample detection, the above various problems will cause the accuracy of the detection result of the instrument to be unable to meet the requirements.

[0006] At present, there is no effective solution to the problem of poor accuracy of the detection result of the sample in the related art.

[0007] Therefore, the utility model provides a sample tube light shielding positioning structure and a multi-index detection qPCR device to overcome the defects of the prior art. Utility model content

[0008] The utility model aims at providing a sample tube light shielding positioning structure which can shield light for the sample tube and improve the accuracy of the detection result.

[0009] Another purpose of the utility model is to provide a multi-index detection qPCR device which can realize on-site detection of large quantities of samples, and the detection experiment of the sample does not need to wait, so as to speed up the production speed of the detection result of the sample.

[0010] The purpose of the utility model can be realized by the following scheme:

[0011] The utility model provides a sample tube light shielding positioning structure, the sample tube light shielding positioning structure includes:

[0012] The positioning table is provided with a limiting groove on the top, a plurality of plungers are arranged on the side wall of the limiting groove and protrude to the inside of the limiting groove, a plurality of positioning grooves are sequentially arranged on the bottom wall of the limiting groove and along the length direction of the limiting groove, a plurality of positioning through holes are respectively arranged in the plurality of positioning grooves, and the positioning through holes are used for containing sample tubes;

[0013] A plurality of light shielding covers, each of the plurality of light shielding covers is arranged above a plurality of positioning grooves, the light shielding cover is used for arranging a sample tube in the positioning through hole to the inner side of the light shielding cover, and the outer wall of the light shielding cover is provided with a plunger clamping groove, and the plunger is clamped in the plunger clamping groove in the corresponding position.

[0014] In a preferred embodiment of the utility model, the light shielding cover comprises a cover body, the cover body is a cylindrical structure with a top sealed and a bottom opened, and the plunger clamping groove is located on the outer wall of the cover body and is an annular groove extending along the circumferential direction of the cover body partially or entirely.

[0015] When the plunger is clamped in the plunger clamping groove, the outer wall of the cover body is tightly attached to the side wall of the positioning groove, and the bottom of the cover body is embedded in the corresponding positioning groove.

[0016] In a preferred embodiment of the utility model, the positioning through hole is used for extending the lower part of the sample tube arranged therein to the lower part of the positioning table, so that the heating and refrigerating member located below the positioning table can adjust the temperature of the sample to be detected in the sample tube.

[0017] The utility model provides a kind of multi-index detection qPCR device, and the multi-index detection qPCR device includes:

[0018] The above-mentioned sample tube light shielding positioning structure;

[0019] A plurality of temperature control amplification components, each of the plurality of temperature control amplification components corresponds to a plurality of positioning grooves in the sample tube light shielding positioning structure, the lower part of the sample tube in each of the plurality of positioning grooves can extend into the corresponding temperature control amplification component, and each of the plurality of temperature control amplification components can be independently temperature-controlled to amplify the sample to be detected in the corresponding sample tube;Each of the plurality of temperature control amplification components is provided with a light passing portion.

[0020] An optical detection component, the optical detection component is movably arranged on one side of the temperature control amplification component along the length direction of the positioning table in the sample tube light shielding positioning structure, and the optical detection component is used to collect the fluorescence signal emitted by the amplified sample in the corresponding sample tube through the light passing portion.

[0021] In a preferred embodiment of the utility model, the temperature control amplification component includes a positioning plate and a heating and refrigerating member, the positioning plate is provided with a plurality of positioning barrels for accommodating the sample tube, the top of the positioning barrel is opened, the bottom of the positioning barrel is connected with the top surface of the positioning plate, and the plurality of positioning barrels are staggered in the length direction of the positioning table.

[0022] The heating and refrigerating part is located below the positioning plate and connected with the bottom surface of the positioning plate, and is used for heating or cooling the sample tube according to the replication and amplification stage of the sample to be detected.

[0023] In a preferred embodiment of the present application, the temperature control amplification assembly further comprises a heat shield, the lower part of the heat shield is formed with an open cavity, and the upper part of the heat shield has a plurality of through holes corresponding to the positioning cylinders.

[0024] The heat shield is arranged outside the positioning plate and the heating and refrigerating part, so that the plurality of positioning cylinders on the positioning plate are located in the cavity, and the plurality of through holes are respectively in communication with the top openings of the corresponding plurality of positioning cylinders.

[0025] In a preferred embodiment of the present application, the top surface of the positioning plate is provided with a light guide block, and a plurality of first light passing holes are arranged on the light guide block and opposite to the plurality of positioning cylinders along a straight line.

[0026] The plurality of first light passing holes directly penetrate the side walls of the corresponding positioning cylinders and are in communication with the interiors of the corresponding positioning cylinders, and / or the plurality of first light passing holes are respectively connected with the side walls of the corresponding positioning cylinders through light guide members, and the light guide members penetrate the side walls of the positioning cylinders.

[0027] The side wall of the heat shield has a second light passing hole, and the second light passing hole is in communication with the plurality of first light passing holes, and the second light passing hole cooperates with the first light passing holes to form the light passing part.

[0028] In a preferred embodiment of the present application, the multi-index detection qPCR device further comprises a synchronous belt and a slide rail, the synchronous belt and the slide rail are arranged along the length direction of the positioning table, the slide rail is provided with a slidable sliding block, the sliding block is connected with the synchronous belt, the optical detection assembly is arranged on the sliding block, and the synchronous belt drives the sliding block to move along the slide rail, so that the optical detection assembly moves to the position where the temperature control amplification assembly is located.

[0029] In a preferred embodiment of the present application, the optical detection assembly comprises an optical scanning head, the optical scanning head has a plurality of independent optical channels, the optical scanning head emits excitation light to the corresponding plurality of sample tubes through the plurality of optical channels, and receives the fluorescence signals emitted by the corresponding plurality of sample tubes through the plurality of optical channels.

[0030] In a preferred embodiment of the utility model, the optical channel includes excitation channel, emission channel and common channel, the extension direction of emission channel and the extension direction of common channel are on the same straight line, and the extension direction of excitation channel and the extension direction of common channel are perpendicular.

[0031] One end of excitation channel and one end of emission channel are respectively provided with light source and signal collector, the other end of excitation channel and the other end of emission channel are all communicated with one end of common channel, the other end of common channel is provided with first lens, dichroic mirror is arranged on the position of excitation channel, emission channel and common channel communication, the dichroic mirror is gradually arranged to the direction of away from excitation channel direction from emission channel to common channel direction, and the dichroic mirror is used to reflect the excitation light in excitation channel to common channel and be shot out by first lens, and the dichroic mirror is used to transmit the fluorescence signal into common channel to emission channel and be received by signal collector.

[0032] In a preferred embodiment of the utility model, the second lens and excitation filter are arranged at the position close to dichroic mirror in excitation channel.

[0033] And / or, the third lens and emission filter are arranged at the position close to dichroic mirror in emission channel.

[0034] In a preferred embodiment of the utility model, the optical detection assembly further includes first heating plate, and the first heating plate is arranged on the outer wall of optical scanning head and close to the position of excitation channel.

[0035] And / or, the optical detection assembly further includes second heating plate, and the second heating plate is arranged on the outer wall of optical scanning head and close to the position of emission channel.

[0036] From the above, the characteristics and advantages of the sample tube light shielding positioning structure and the device for detecting multiple indexes of qPCR of the utility model are:

[0037] The positioning groove is provided on the top of the positioning table, a plurality of positioning grooves are sequentially arranged on the bottom wall of the positioning groove and along the length direction of the positioning groove, and a plurality of positioning through holes are respectively arranged in the plurality of positioning grooves; when the sample to be detected is detected, the sample tube storing the sample to be detected can be placed in the positioning through hole, so that the sample tube is stably positioned; in addition, the light shielding cover is respectively arranged above each positioning groove, so that the sample tube located in the positioning through hole is covered on the inner side of the light shielding cover; in the installed state of the light shielding cover, the plunger located on the side wall of the positioning groove is clamped in the plunger clamping groove of the corresponding position of the light shielding cover, the positioning of the light shielding cover is realized, the light shielding cover can play a good light shielding effect on the sample tube, the light or other stray light in the external environment is prevented from interfering with the detection, and the accuracy of the detection result is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0038] The following drawings merely aim to schematically illustrate and explain the present application and do not limit the scope of the present application. Among them:

[0039] Figure 1 It is a structural schematic view of the sample tube light shielding positioning structure of the present application;

[0040] Figure 2 It is a structural schematic view of the light shielding cover in the sample tube light shielding positioning structure of the present application;

[0041] Figure 3 It is one of the perspective views of the multi-index detection qPCR device of the present application;

[0042] Figure 4 It is a partial sectional view of the multi-index detection qPCR device of the present application;

[0043] Figure 5 It is a schematic view of the temperature control amplification assembly in the multi-index detection qPCR device of the present application in a disassembled state;

[0044] Figure 6 It is a schematic view of the temperature control amplification assembly in the multi-index detection qPCR device of the present application in an assembled state;

[0045] Figure 7 It is the second perspective view of the multi-index detection qPCR device of the present application;

[0046] Figure 8 It is a perspective view of the optical detection assembly in the multi-index detection qPCR device of the present application;

[0047] Figure 9 It is a sectional view of the optical detection assembly in the multi-index detection qPCR device of the present application at the optical channel position.

[0048] In the present application, the reference signs are:

[0049] 1, positioning table; 101, limiting groove;

[0050] 102, positioning groove; 103, positioning through hole;

[0051] 104, plunger; 2, light shielding cover;

[0052] 201, cover; 202, plunger slot;

[0053] 203, cover handle; 3, sample tube;

[0054] 10, sample tube light shielding positioning structure; 20, temperature control amplification assembly;

[0055] 2001, positioning plate; 2002, heating and refrigeration element;

[0056] 2003, positioning cylinder; 2004, heat shield;

[0057] 2005, through hole; 2006, heat transfer element;

[0058] 2007, light guide block; 2008, first light passing hole;

[0059] 2009, light guide element; 2010, second light passing hole;

[0060] 30, optical detection assembly; 3001, optical scanning head;

[0061] 3002, optical channel; 30021, light source;

[0062] 30022, second lens; 30023, excitation filter;

[0063] 30024, dichroic mirror; 30025, first lens;

[0064] 30026, emission filter; 30027, third lens;

[0065] 30028, signal collector; 30029, excitation channel;

[0066] 30030, emission channel; 30031, common channel;

[0067] 3003, first heating plate; 3004, second heating plate;

[0068] 40, heat sink; 50, heat conduction block;

[0069] 60, synchronous belt; 70, slide rail;

[0070] 80, slider; 90, mounting plate;

[0071] 100, drive wheel; 110, driven wheel;

[0072] 120, motor. DETAILED DESCRIPTION

[0073] The technical solutions of the present application will be described in detail below with reference to the drawings and specific embodiments. It should be understood that these embodiments are only used to illustrate the present application and not to limit the scope of the present application. After reading the present application, those skilled in the art can make various equivalent modifications to the present application, which all fall within the scope defined by the appended claims.

[0074] It should be noted that when an element is referred to as "disposed on" another element, it can be directly on the other element or there can be an intervening element. When an element is referred to as "connected" to another element, it can be directly connected to the other element or there can be an intervening element. The terms "vertical", "horizontal", "upper", "lower", "left", "right", and similar terms as used herein are for purposes of description only and are not intended to be limiting.

[0075] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0076] Embodiment one

[0077] As shown in Figure 1 and Figure 2 The present application provides a sample tube light shielding positioning structure, which comprises a positioning table 1 and a plurality of light shielding covers 2. The positioning table 1 is a long strip-shaped boss. The top of the positioning table 1 has a long strip-shaped limiting groove 101 extending along the length direction of the positioning table 1. A plurality of plungers 104 are arranged on the side wall of the limiting groove 101 and protrude inwardly. A plurality of positioning grooves 102 are sequentially arranged on the bottom wall of the limiting groove 101 along the length direction of the limiting groove 101. A plurality of positioning through holes 103 are arranged in the plurality of positioning grooves 102, respectively. The positioning through holes 103 are used for accommodating sample tubes 3. The plurality of light shielding covers 2 correspond to the plurality of positioning grooves 102 one by one. The plurality of light shielding covers 2 are respectively arranged above the corresponding plurality of positioning grooves 102. The light shielding cover 2 is used for covering the sample tube 3 in the positioning through hole 103 inwardly. The outer wall of the light shielding cover 2 has a plunger clamping groove 202. The plunger 104 is clamped in the plunger clamping groove 202 of the light shielding cover 2 at the corresponding position.

[0078] The utility model discloses a plurality of positioning slots 102 are sequentially arranged on the bottom wall of the limiting groove 101 and along the length direction of the limiting groove 101, and a plurality of positioning through holes 103 are arranged in the plurality of positioning slots 102 respectively, when detecting the sample to be measured, the sample tube 3 for storing the sample to be measured can be placed in the positioning through hole 103, so that the plurality of sample tubes 3 can be positioned stably at the same time, in addition, the light shielding cover 2 is respectively covered above each positioning slot 102, so that the sample tube 3 in the positioning through hole 103 is covered on the inner side of the light shielding cover 2, under the installation state of the light shielding cover 2, the plunger 104 on the side wall of the limiting groove 101 is connected in the plunger card slot 202 of the light shielding cover 2 in the corresponding position, the positioning of the light shielding cover 2 is realized, the light shielding effect of the light shielding cover 2 on the sample tube 3 is guaranteed, the light or other stray light in the external environment is prevented from interfering with the detection, and the accuracy of the detection result is guaranteed.

[0079] In an optional embodiment of the utility model, as shown in Figure 1 、 Figure 2 The light shielding cover 2 includes a cover body 201 and a cover handle 203, the cover body 201 is a cylindrical structure with a top opening and a bottom opening, the cover handle 203 is arranged at the top position of the cover body 201, preferably at the middle position of the top of the cover body 201, and the plunger card slot 202 is arranged on the outer wall of the cover body 201 and is an annular slot extending along the circumference of the cover body 201; when the plunger 104 is connected in the plunger card slot 202, the outer wall of the cover body 201 is tightly attached to the side wall of the positioning slot 102, and the bottom of the cover body 201 is embedded in the corresponding positioning slot 102, so that the installation and limiting of the light shielding cover 2 are realized, and the stable light shielding of the sample tube 3 is guaranteed.

[0080] Preferably, the plunger card slot 202 can be an annular slot extending along the circumference of the cover body 201, so that the light shielding cover 2 does not need to consider the direction when covering, and the plunger card slot 202 and the plunger 104 can be connected and matched at will, which is convenient to operate. Of course, it can also be a ring-shaped or other-shaped and sized card slot, which can be stably connected with the plunger 104, and the specific shape and size of the plunger card slot 202 are not limited in the utility model.

[0081] Further, the plunger 104 is a ball head plunger (i.e. the end of the plunger 104 is spherical or hemispherical) with a certain elasticity, so that it can be deformed to a certain extent after being pressed, so that the plunger 104 can be smoothly clamped into the plunger clamping groove 202 during the installation of the light shielding cover 2. When the light shielding cover 2 is embedded and installed into the positioning groove 102, the outer wall of the cover body 201 will press the plunger 104 to deform; when the light shielding cover 2 continues to be embedded and installed into the positioning groove 102, the plunger 104 enters the plunger clamping groove 202, at this time, under the action of the elastic restoring force of the plunger 104 itself, the plunger 104 restores to its original shape and is clamped in the plunger clamping groove 202, thereby stably fixing the light shielding cover 2 in the positioning groove 102 and preventing the light shielding cover 2 from shaking; since the plunger 104 will generate a force (i.e. downward force) towards the positioning table 1, the top inner wall of the light shielding cover 2 can be in close contact with the top of the sample tube 3 located inside, so that the sample tube 3 is firmly placed in the corresponding position, and the heat conduction efficiency is improved.

[0082] Further, the positioning through hole 103 is used for the lower part of the sample tube 3 contained therein to extend below the positioning table 1, so that the heating and refrigeration member 2002 located below the positioning table 1 can adjust the temperature of the sample to be detected in the sample tube 3. The arrangement of the light shielding cover 2 can also press down the sample tube 3, so that the lower part of the sample tube 3 can be in close contact with the heating and refrigeration member 2002 and the corresponding heat transfer structure, ensuring rapid heat transfer of the sample tube 3, and thereby improving the accuracy of detection of the sample to be detected in the sample tube 3.

[0083] The characteristics and advantages of the sample tube light shielding and positioning structure of the utility model are:

[0084] I. In the sample tube light shielding and positioning structure, the light shielding cover 2 can have a good light shielding effect on the sample tube 3, preventing external light or other stray light from interfering with the detection, and ensuring the accuracy of the detection result.

[0085] II. In the sample tube light shielding and positioning structure, the light shielding cover 2 can press down the sample tube 3, so that the lower part of the sample tube 3 can be in close contact with the heating and refrigeration member 2002 and the corresponding heat transfer structure, ensuring rapid heat transfer of the sample tube 3 and improving the accuracy of detection of the sample to be detected in the sample tube 3.

[0086] Embodiment two

[0087] As Figures 1 to 9As shown, the utility model provides a kind of multi-index detection qPCR's device, the multi-index detection qPCR's device includes optical detection component 30, multiple temperature control amplification components 20 and above-mentioned sample tube light shielding positioning structure 10, multiple temperature control amplification components 20 respectively with multiple positioning slots 102 in sample tube light shielding positioning structure 10 corresponding, the lower part of sample tube 3 in multiple positioning slots 102 can be extended into corresponding temperature control amplification component 20, multiple temperature control amplification components 20 can be respectively independently temperature-regulated, to respectively with the sample tube 3 in the temperature control amplification component 20 corresponding sample to be measured in amplification;Multiple temperature control amplification components 20 are respectively provided with light passing portion;Optical detection component 30 can be movably arranged in the length direction of positioning table 1 in sample tube light shielding positioning structure 10 in one side of temperature control amplification component 20, and when optical detection component 30 moves in the length direction of positioning table 1 state, optical detection component 30 is used to collect the fluorescence signal emitted by the sample amplified in corresponding sample tube 3 by the light passing portion of temperature control amplification component 20.

[0088] In the utility model, the length direction of positioning table 1 can be any straight line or arc direction, preferably a straight line direction, i.e. multiple temperature control amplification components 20 can be sequentially arranged along a straight line direction, when optical detection component 30 reciprocates along the straight line direction, the corresponding sample tube 3 in the multiple temperature control amplification components 20 arranged in the straight line direction can emit excitation light and receive the fluorescence signal emitted by the sample to be measured. By this method, the fluorescence signal of each sample to be measured after amplification cycle can be obtained and finally an amplification curve graph is generated, and the pathological condition of the patient can be reflected by the amplification curve graph.

[0089] In one embodiment of the utility model, as shown in Figure 3 And Figure 4 As shown, the number of multiple positioning slots 102 and temperature control amplification components 20 is taken as an example to introduce the device of the utility model, which is not limited to 12, and the number of positioning slots 102 and temperature control amplification components 20 can be more or less, which can be set according to the actual number of samples to be measured to meet the batch processing requirements.

[0090] In the utility model, multiple temperature control amplification assemblies 20 respectively carry out independent temperature adjustment, and the to-be-tested sample added into the sample tube 3 corresponding to the temperature control amplification assembly 20 is separately and quickly copied and amplified, so that the to-be-tested sample is tested as it comes, even if the to-be-tested sample is large in quantity and cannot be sent for testing at the same time, the to-be-tested sample that is sent does not need to wait, so that the speed of outputting the detection result of the to-be-tested sample is greatly increased; in addition, the light passing part is arranged on each temperature control amplification assembly 20, when the optical detection assembly 30 moves, the excitation light emitted by the optical detection assembly 30 passes through the light passing part on the corresponding temperature control amplification assembly 20 to the to-be-tested sample in the sample tube 3, and the fluorescence signal (i.e. emission light) emitted by the to-be-tested sample is received, so that the fluorescence signal emitted by the sample amplified in the multiple sample tubes 3 can be collected, the fluorescence signal is more stable, the accuracy of the detection result is ensured, and the occurrence of the misjudgment of the detection result is avoided. In the utility model, each temperature control amplification assembly 20 can be started alone at any time, so that the to-be-tested sample can be detected alone, and the to-be-tested samples do not affect each other, the detection wave band of the excitation light and / or the emission light can be adjusted by the optical detection assembly 30, so that the detection of the multiple target genes of the single to-be-tested sample can be realized, the to-be-tested sample does not need to be collected multiple times and the multiple target genes do not need to be detected multiple times, the detection efficiency is improved, and the multi-channel detection requirement is met.

[0091] In the actual application scene (such as a hospital), the to-be-tested samples of different patients are sent for testing at different times, and the to-be-tested samples of different sending times can be tested as they come, on the one hand, different from the traditional high-throughput PCR instrument, the multi-index detection qPCR device of the utility model does not need to wait for the to-be-tested sample quantity to accumulate to a certain amount before starting detection, and also different from the traditional to-be-tested sample as it comes, the multi-index detection qPCR device of the utility model does not need to wait for the detection of the previous to-be-tested sample to be completed before detecting the next to-be-tested sample, therefore, the speed of outputting the detection result of the to-be-tested sample can be greatly increased, and the pathological analysis of the patient can be conveniently and timely performed; meanwhile, the simultaneous detection of the multiple target genes of the single to-be-tested sample can be realized, the to-be-tested sample of the patient does not need to be collected multiple times for the detection of the different target genes, the detection efficiency is improved, and the pain of the patient caused by collecting the sample multiple times is avoided.

[0092] In an optional embodiment of the utility model, Figure 5 and Figure 6As shown, the temperature control amplification assembly 20 comprises a positioning plate 2001 and a heating refrigeration element 2002, the positioning plate 2001 is arranged in the horizontal direction, a plurality of positioning barrels 2003 for accommodating the sample tube 3 are arranged on the top surface of the positioning plate 2001, the top of the positioning barrel 2003 is open, the bottom of the positioning barrel 2003 is connected with the top surface of the positioning plate 2001, and the plurality of positioning barrels 2003 are staggered in the length direction of the positioning table 1; the heating refrigeration element 2002 is located below the positioning plate 2001 and is connected with the bottom surface of the positioning plate 2001, and the heating refrigeration element 2002 is used for heating or cooling treatment of the sample tube 3 according to the replication amplification stage of the sample to be tested. Wherein, the positioning plate 2001 and the positioning barrel 2003 can be positioned on the sample tube 3, and the sample tube 3 can be quickly placed in place.

[0093] In one embodiment of the present application, as shown in Figure 5 and Figure 6 each positioning plate 2001 has four positioning barrels 2003, and two of them form two rows, and the four positioning barrels 2003 are staggered in the length direction of the positioning table 1, so that the fluorescence information emitted by the sample to be tested in each positioning barrel 2003 can be smoothly received.

[0094] Wherein, the heating refrigeration element 2002 can adopt but is not limited to Peltier.

[0095] Further, as shown in Figure 5 and Figure 6 the temperature control amplification assembly 20 further comprises a heat shield 2004, the lower part of the heat shield 2004 forms an open cavity, the upper part of the heat shield 2004 has a plurality of through holes 2005 corresponding to the positioning barrels 2003, and the plurality of through holes 2005 correspond one-to-one to the plurality of positioning barrels 2003; the heat shield 2004 is arranged outside the positioning plate 2001 and the heating refrigeration element 2002, so that the plurality of positioning barrels 2003 on the positioning plate 2001 are located in the cavity, and the plurality of through holes 2005 are respectively in communication with the top openings of the corresponding plurality of positioning barrels 2003, so that when the sample tube 3 is placed, the lower part of the sample tube 3 can pass through the through hole 2005 and enter the corresponding positioning barrel 2003, realizing stable placement of the sample tube 3. The heat shield 2004 has the function of heat insulation, which can prevent temperature interference between the adjacent two temperature control amplification assemblies 20 and avoid affecting the PCR amplification of the sample to be tested.

[0096] Further, as shown in Figure 5As shown, the heat transfer piece 2006 is arranged between the positioning plate 2001 and the heating and refrigeration piece 2002. The heat transfer piece 2006 is in a sheet shape, and the top surface and the bottom surface of the heat transfer piece 2006 are fully attached to the positioning plate 2001 and the heating and refrigeration piece 2002 respectively, so as to achieve good heat transfer effect, improve heat transfer efficiency, accelerate the heating or cooling rate, and shorten the detection time. The heat transfer piece 2006 can be, but is not limited to, a heat-conducting carbon film. The heat-conducting carbon film is attached between the positioning plate 2001 and the heating and refrigeration piece 2002, so that the heat transfer efficiency can be improved through the heat-conducting carbon film. The heat-conducting carbon film is arranged because any one plane cannot form an absolutely smooth plane during production and manufacturing (the surface will have fine pits and / or protrusions). When two planes are attached to each other, the pits and / or protrusions on the planes will cause a gap between the two planes and cannot be fully attached, which will lead to a decrease in the heat transfer efficiency between the two planes. In the utility model, the heat-conducting carbon film is arranged between the two planes, so as to fill the pits and / or protrusions between the positioning plate 2001 and the heating and refrigeration piece 2002, and thus the heat transfer efficiency between the two planes is improved.

[0097] When the utility model detects the sample to be measured, a plurality of sample tubes 3 containing the same patient's sample to be measured are sequentially placed in a plurality of positioning barrels 2003 on a positioning plate 2001 through a plurality of through holes 2005, and then the heating and refrigeration piece 2002 performs a cycle operation of heating and cooling, so as to conduct heat to the sample to be measured in the sample tube 3 through the positioning barrel 2003 or dissipate heat to the environment through the positioning barrel 2003. One temperature control amplification assembly 20 in the utility model can receive a plurality of (such as 4) sample tubes 3 containing the sample to be measured of one patient at most once. Since the PCR reaction system contained in each sample tube 3 is different, the multi-index detection of the same patient can be realized. When the sample tube 3 is not received, the temperature control amplification assembly 20 is in a stop working state, and the amplification operation is started only when the sample tube 3 is received.

[0098] In an optional embodiment of the utility model, as shown in Figure 3 and Figure 4 As shown, the multi-index detection qPCR device further comprises a heat sink 40, the heat sink 40 is provided with a heat-conducting block 50 extending along the length direction of the positioning table 1, the sample tube light-shielding positioning structure 10 is arranged on the top of the heat-conducting block 50, and the heating and refrigeration piece 2002 is attached to the top of the heat-conducting block 50. Through the cooperation of the heat sink 40 and the heat-conducting block 50, heat can be quickly dissipated to the external environment during the cooling process, and the temperature control rate is effectively improved.

[0099] In an optional embodiment of the utility model, as shown in Figure 5As shown, the top surface of the positioning plate 2001 is provided with a light guide block 2007, and the light guide block 2007 is provided with a plurality of first light transmission holes 2008 corresponding to the plurality of positioning cylinders 2003 along a straight line. Since the positioning cylinders 2003 are arranged in front and back rows, the positioning cylinders 2003 close to the light guide block 2007 are directly penetrated by the plurality of first light transmission holes 2008 and are in communication with the inside of the corresponding positioning cylinder 2003. The positioning cylinders 2003 away from the light guide block 2007 are connected to the side wall of the corresponding positioning cylinder 2003 through the light guide piece 2009, and the light guide piece 2009 penetrates the side wall of the positioning cylinder 2003. Therefore, through the arrangement of the plurality of first light transmission holes 2008, the excitation light emitted by the optical detection assembly 30 can be transmitted to the sample tube 4, and the fluorescent signal (emitted light) emitted by the sample tube 4 can be transmitted to the optical detection assembly 30 through the corresponding first light transmission hole 2008, so that the fluorescent signal can be received, and the pathological analysis of the patient can be completed by collecting the fluorescent signal and forming an amplification curve graph. The light guide piece 2009 can be but is not limited to a hard glass optical fiber or a light guide column.

[0100] Further, as shown in Figure 5 and Figure 6 , the side wall of the heat shield 2004 has a second light transmission hole 2010, and the second light transmission hole 2010 is in communication with the plurality of first light transmission holes 2008. The second light transmission hole 2010 and the first light transmission hole 2008 cooperate to form a light transmission part.

[0101] In an optional embodiment of the present application, as shown in Figure 4 and Figure 7 , the multi-index detection qPCR device further comprises a synchronous belt 60 and a sliding rail 70, the synchronous belt 60 and the sliding rail 70 are arranged along the length direction of the positioning table 1, the sliding rail 70 is provided with a slidable sliding block 80, the sliding block 80 is connected with the synchronous belt 60, the optical detection assembly 30 is arranged on the sliding block 80, the synchronous belt 60 drives the sliding block 80 to move along the sliding rail 70, so as to control the movement of the optical detection assembly 30, and then the optical detection assembly 30 can be moved to the position of the corresponding temperature control amplification assembly 20, and the excitation light can be transmitted to the corresponding sample tube 3 or the fluorescent signal emitted by the sample in the corresponding sample tube 3 can be received through the first light transmission hole 2008.

[0102] Specifically, as shown in Figure 4 and Figure 7As shown in the drawings, one side of the sample tube light shielding positioning structure 10 is provided with a mounting plate 90 in a horizontal direction, the top of the mounting plate 90 is provided with a driving wheel 100 and a driven wheel 110, the two ends of the synchronous belt 60 are connected to the driving wheel 100 and the driven wheel 110 respectively, the bottom of the mounting plate 90 is provided with a motor 120, the output shaft of the motor 120 is connected with the center shaft of the driving wheel 100, and the slide rail 70 is arranged on the top of the mounting plate 90. The motor 120 drives the synchronous belt 60 to move, so that the optical detection assembly 30 can pass through each temperature control amplification assembly 20 in turn and complete the collection of the fluorescence signal, and the motor 120 alternately rotates forward and reversely, so that the reciprocating movement of the optical detection assembly 30 along the arrangement direction of the multiple temperature control amplification assemblies 20 can be completed, and the collection of the fluorescence signal of the sample to be detected in the different sample tubes 3 can be completed.

[0103] In an optional embodiment of the present application, as shown in Figure 8 and Figure 9 The optical detection assembly 30 comprises an optical scanning head 3001, the optical scanning head 3001 is provided with a plurality of independent optical channels 3002, the optical scanning head 3001 emits excitation light to the corresponding multiple sample tubes 3 through the multiple optical channels 3002 respectively, and receives the fluorescence signals emitted by the corresponding multiple sample tubes 3 through the multiple optical channels 3002 respectively. In the detection process, each optical channel 3002 can detect one index, so the number of optical channels 3002 on the optical scanning head 3001 is positively correlated with the upper limit of multi-index detection. The multiple optical channels 3002 in the present application are isolated by physical structures (such as a partition plate and the like), so that the problem of fluorescence signal crosstalk can be avoided, and the accuracy of the detection result is improved.

[0104] Specifically, as shown in Figure 9As shown, the optical channel 3002 includes an excitation channel 30029, an emission channel 30030, and a common channel 30031, the extension direction of the emission channel 30030 is on the same straight line with the extension direction of the common channel 30031, and the extension direction of the excitation channel 30029 is perpendicular to the extension direction of the common channel 30031; one end of the excitation channel 30029 is provided with a light source 30021, one end of the emission channel 30030 is provided with a signal collector 30028, the other end of the excitation channel 30029 and the other end of the emission channel 30030 are both in communication with one end of the common channel 30031, the other end of the common channel 30031 is provided with a first lens 30025, a dichroic mirror 30024 is arranged at the position where the excitation channel 30029, the emission channel 30030 and the common channel 30031 are in communication, the dichroic mirror 30024 is gradually arranged to be inclined away from the excitation channel 30029 from the emission channel 30030 to the common channel 30031, the excitation light (emitted by the light source 30021) in the excitation channel 30029 can be reflected to the common channel 30031 by the dichroic mirror 30024 and emitted by the first lens 30025, the dichroic mirror 30024 can also transmit the fluorescent signal entering the common channel 30031 to the emission channel 30030 and be received by the signal collector 30028.

[0105] Further, as shown in Figure 9 the excitation channel 30029 and close to the dichroic mirror 30024 are provided with a second lens 30022 and an excitation filter 30023; the emission channel 30030 and close to the dichroic mirror 30024 are provided with a third lens 30027 and an emission filter 30026. When detecting the to-be-detected sample, the light source 30021 emits excitation light, which is collimated as parallel light after passing through the second lens 30022, and then reaches the dichroic mirror 30024 after filtering out the light of the unnecessary wavelength by the excitation filter 30023, and is reflected to the first lens 30025, the light is concentrated and converged by the first lens 30025, and then passes through the light passing part of the temperature-controlled amplification assembly 20 to reach the to-be-detected sample, the to-be-detected sample is excited to generate emission light, the emission light passes through the light passing part of the temperature-controlled amplification assembly 20, the first lens 30025, the dichroic mirror 30024, the emission filter 30026, and the third lens 30027 in sequence, is converged, and then reaches the signal collector 30028 to be collected, thereby completing the reception and collection of the fluorescent signal.

[0106] Further, as shown in Figure 7 and Figure 8As shown, the optical detection assembly 30 further comprises a first heating plate 3003 arranged on the outer wall of the optical scanning head 3001 and close to the excitation channel 30029, because the excitation light emitted by the light source 30021 will fluctuate after being affected by temperature, so that the temperature of the environment (at least including the excitation channel 30029) where the light source 30021 is located is guaranteed to be a constant temperature environment by arranging the first heating plate 3003, thereby reducing the fluctuation of the excitation light; the optical detection assembly 30 further comprises a second heating plate 3004 arranged on the outer wall of the optical scanning head 3001 and close to the emission channel 30030, and for the same reason, the signal collector 30028 is also easily affected by temperature to cause the collected fluorescence signal to fluctuate, so that the temperature of the environment (at least including the emission channel 30030) where the signal collector 30028 is located is guaranteed to be a constant temperature environment by arranging the second heating plate 3004, thereby reducing the fluctuation of the fluorescence signal.

[0107] The device for multi-index detection qPCR has the following characteristics and advantages:

[0108] I. In the device for multi-index detection qPCR, the plurality of temperature control amplification assemblies 20 are independently temperature-controlled, and the to-be-detected samples added into the sample tubes 3 corresponding to the temperature control amplification assemblies 20 are individually and rapidly copied and amplified, so that the to-be-detected samples are detected as soon as they are received, and even in the case that the number of to-be-detected samples is large and cannot be sent for detection at the same time, the to-be-detected samples received in time do not need to be waited for, and the speed of outputting the detection results of the to-be-detected samples is greatly accelerated.

[0109] II. The device for multi-index detection qPCR can collect the fluorescence signals emitted by the samples amplified in the plurality of sample tubes 3, the fluorescence signals are more stably received, the accuracy of the detection results is guaranteed, and the occurrence of false detection results is avoided.

[0110] III. In the device for multi-index detection qPCR, each temperature control amplification assembly 20 can be individually started at any time, so that the to-be-detected samples can be individually detected without affecting each other, the detection wavelength bands of the excitation light and / or the emission light can be adjusted by the optical detection assembly 30, so that the detection of a plurality of target genes of a single to-be-detected sample is realized, the to-be-detected samples do not need to be collected multiple times and the target genes do not need to be detected multiple times, the detection efficiency is improved, and the multi-channel detection requirement is met.

[0111] In addition, the device for multi-index detection qPCR also has the same characteristics and advantages as the above-mentioned sample tube light-shielding positioning structure, which will not be described here.

[0112] It should be noted that in the description of the present application, the terms "first", "second" and the like are used only for descriptive purposes and to distinguish similar objects, and there is no precedence between them, nor can it be understood as indicating or implying relative importance. In addition, in the description of the present application, unless otherwise stated, the meaning of "a plurality of" is two or more.

[0113] The above various embodiments in the specification are described in a progressive manner, and the same or similar parts between the various embodiments can be referred to each other, and each embodiment focuses on the difference from other embodiments.

[0114] The above is only a few embodiments of the present application, although the embodiments disclosed by the present application are as above, but the content is only for the purpose of understanding the present application and the embodiments adopted, not for limiting the present application. Any person skilled in the art of the present application, without departing from the spirit and scope of the present application, can make any modification and change in the form and details of the embodiments, but the patent protection scope of the present application shall be subject to the scope defined by the appended claims.

Claims

1. A sample tube light-shielding positioning structure, characterized in that, The sample tube light-shielding positioning structure includes: The positioning platform has a limiting groove on its top, and multiple plungers protruding into the limiting groove are provided on the side wall of the limiting groove. Multiple positioning grooves are arranged sequentially on the bottom wall of the limiting groove along the length direction of the limiting groove. Multiple positioning through holes are provided in the multiple positioning grooves respectively, and the positioning through holes are used to accommodate sample tubes. Multiple light-shielding covers are respectively placed above multiple positioning slots. The light-shielding covers are used to cover the sample tube located in the positioning through hole inside the light-shielding cover. The outer wall of the light-shielding cover has a plunger slot, and the plunger is engaged in the plunger slot of the light-shielding cover at the corresponding position.

2. The sample tube light-shielding positioning structure as described in claim 1, characterized in that, The light-shielding cover includes a cover body, which is a cylindrical structure with a sealed top and an open bottom. The plunger groove is located on the outer wall of the cover body and is an annular groove that extends partially or completely along the circumference of the cover body. When the plunger is engaged in the plunger slot, the outer wall of the cover is in close contact with the side wall of the positioning groove, and the bottom of the cover is embedded in the corresponding positioning groove.

3. The sample tube light-shielding positioning structure as described in claim 1, characterized in that, The positioning through hole is used to allow the lower part of the sample tube contained therein to extend to the bottom of the positioning stage, so that the heating and cooling components located below the positioning stage can regulate the temperature of the sample to be tested inside the sample tube.

4. A device for multi-index detection of qPCR, characterized in that, The device for multi-index detection of qPCR includes: The sample tube light-shielding positioning structure according to any one of claims 1 to 3; Multiple temperature-controlled amplification components are provided, each corresponding to a multiple positioning slot in the sample tube light-shielding positioning structure. The lower part of the sample tube in each positioning slot can extend into the corresponding temperature-controlled amplification component. Each temperature-controlled amplification component can be independently temperature-controlled to amplify the sample to be tested in its corresponding sample tube. Each temperature-controlled amplification component is provided with a light-passing section. An optical detection component is provided, which is movably disposed on one side of the temperature-controlled amplification component along the length of the positioning stage in the sample tube light-shielding positioning structure. The optical detection component is used to collect the fluorescence signal emitted by the amplified sample in the corresponding sample tube through the light-passing part.

5. The apparatus for multi-index detection of qPCR as described in claim 4, characterized in that, The temperature-controlled amplification assembly includes a positioning plate and a heating and cooling component. The positioning plate is provided with multiple positioning cylinders for accommodating the sample tubes. The top of each positioning cylinder is open, and the bottom of each positioning cylinder is connected to the top surface of the positioning plate. The multiple positioning cylinders are staggered along the length of the positioning stage. The heating and cooling element is located below the positioning plate and connected to the bottom surface of the positioning plate. The heating and cooling element is used to heat or cool the sample tube according to the replication and amplification stage of the sample to be tested.

6. The apparatus for multi-index detection of qPCR as described in claim 5, characterized in that, The temperature-controlled amplification assembly also includes a heat shield, the lower part of which forms an open chamber, and the upper part of which has multiple through holes corresponding to the positioning cylinder. The heat insulation cover is provided on the outside of the positioning plate and the heating and cooling components, so that the plurality of positioning cylinders on the positioning plate are located in the cavity, and the plurality of through holes are respectively connected to the top openings of the corresponding plurality of positioning cylinders.

7. The apparatus for multi-index detection of qPCR as described in claim 6, characterized in that, A light guide block is provided on the top surface of the positioning plate, and a plurality of first light-passing holes are provided on the light guide block that are respectively aligned with the plurality of positioning cylinders along a straight line; Multiple first light-passing holes directly penetrate the side wall of the corresponding positioning cylinder and communicate with the interior of the corresponding positioning cylinder, and / or, multiple first light-passing holes are respectively connected to the side wall of the corresponding positioning cylinder through light guides, and the light guides penetrate the side wall of the positioning cylinder; The heat insulation cover has a second light-passing hole on its side wall, and the second light-passing hole communicates with a plurality of first light-passing holes. The second light-passing hole and the first light-passing holes cooperate to form the light-passing part.

8. The apparatus for multi-index detection of qPCR as described in claim 7, characterized in that, The multi-index detection qPCR device further includes a synchronization belt and a slide rail. Both the synchronization belt and the slide rail are arranged along the length of the positioning stage. A sliding slider is provided on the slide rail and is connected to the synchronization belt. The optical detection component is disposed on the slider. The synchronization belt drives the slider to move along the slide rail so that the optical detection component moves to the position of the corresponding temperature-controlled amplification component.

9. The apparatus for multi-index detection of qPCR as described in claim 4, characterized in that, The optical detection assembly includes an optical scanning head, which has multiple independent optical channels. The optical scanning head emits excitation light into the corresponding sample tubes through the multiple optical channels and receives fluorescence signals emitted by the corresponding sample tubes through the multiple optical channels.

10. The apparatus for multi-index detection of qPCR as described in claim 9, characterized in that, The optical channel includes an excitation channel, an emission channel, and a common channel. The extension direction of the emission channel and the extension direction of the common channel are on the same straight line, and the extension direction of the excitation channel is perpendicular to the extension direction of the common channel. A light source and a signal collector are respectively provided at one end of the excitation channel and one end of the emission channel. The other ends of the excitation channel and the emission channel are connected to one end of the common channel. A first lens is provided at the other end of the common channel. A dichroic mirror is provided at the position where the excitation channel, the emission channel and the common channel are connected. The dichroic mirror is gradually tilted away from the excitation channel from the emission channel to the common channel. The dichroic mirror is used to reflect the excitation light in the excitation channel to the common channel and be emitted by the first lens. The dichroic mirror is used to transmit the fluorescence signal entering the common channel to the emission channel and be received by the signal collector.

11. The apparatus for multi-index detection of qPCR as described in claim 10, characterized in that, A second lens and an excitation filter are located within the excitation channel and near the dichroic mirror. And / or, a third lens and an emission filter are located within the emission channel and near the dichroic mirror.

12. The apparatus for multi-index detection of qPCR as described in claim 10, characterized in that, The optical detection assembly further includes a first heating plate, which is disposed on the outer wall of the optical scanning head and near the excitation channel. And / or, the optical detection assembly further includes a second heating plate disposed on the outer wall of the optical scanning head and near the emission channel.

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