Reagent storage and transfer device for pesticide residue detection and pesticide residue detection equipment

Abstract

The utility model relates to the technical field of pesticide residue detection, in particular to a reagent storage and transfer device for pesticide residue detection and pesticide residue detection equipment. The reagent storage and transfer device comprises a reagent storage module, a liquid sample transfer module and an enzyme storage module; a reagent tray of the reagent storage module is used for storing reagent bottles; an enzyme storage bottle of the enzyme storage module is placed in an enzyme storage position of the reagent disc; a mechanical arm of the liquid sample transfer module can drive a liquid adding needle to rotate, so that the liquid adding needle reaches a position above a reagent position and an enzyme storage position, and can also drive the liquid adding needle to descend into a reagent bottle to extract a reagent, and the mechanical arm drives the liquid adding needle to descend into an enzyme storage bottle to extract an enzyme. Compared with a traditional enzyme storage scheme, according to the technical scheme, the enzyme storage positions and the reagent positions are both designed on the reagent disc, portable storage and automatic extraction of enzyme are achieved, carrying convenience can be improved, detection errors caused by the manipulation problem of an operator can be reduced, the pesticide residue detection accuracy is high, and the detection efficiency is high.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of pesticide residue detection, in particular to a reagent storage and transfer device for pesticide residue detection and pesticide residue detection equipment. BACKGROUND

[0002] Pesticides are widely used in the prevention and treatment of crop diseases and insect pests due to their advantages of high efficiency, rapidness, economy and convenience, but also cause pesticide residues on agricultural products to threaten food safety, therefore, it is necessary to detect pesticide residues on agricultural products by pesticide residue detection equipment. When detecting organophosphorus and carbamate pesticide residues, acetylcholinesterase (AChE) is often used as a detection tool, and pesticides can inhibit the activity of acetylcholinesterase, and the presence of these pesticides in the sample and their concentration can be indirectly known by detecting the change of enzyme activity. Generally, in the pesticide residue detection process, a sample to be tested is first prepared, and then the enzyme and substrate are added to the sample to be tested before pesticide residue detection. Enzyme is a biological catalyst, which is often used as a special reagent to catalyze specific chemical reactions, and usually needs to be stored at low temperature to ensure activity, so a separate special storage bottle is usually used for storage.

[0003] In some application scenarios that require real-time pesticide residue detection, both pesticide residue detection equipment and special enzyme storage devices need to be carried, which may cause the following problems: 1. diversification of articles, inconvenience of carrying; 2. in the pesticide residue detection process, the enzyme in the enzyme storage bottle needs to be manually extracted and added to the sample to be tested by the staff, which may cause operation errors and affect the accuracy of pesticide residue detection; 3. inconvenient operation may affect the efficiency of pesticide residue detection. UTILITY MODEL CONTENT

[0004] To overcome the problems in the related art, the utility model provides a reagent storage and transfer device for pesticide residue detection and pesticide residue detection equipment, the reagent storage and transfer device and the reagent storage device are designed in an integrated manner, which is convenient to carry and operate, can reduce detection errors caused by the operation method of the operator, and improve the accuracy and efficiency of pesticide residue detection.

[0005] To achieve the above purpose, the utility model provides a reagent storage and transfer device for pesticide residue detection, which comprises:

[0006] The reagent storage module comprises a reagent disc, a liquid sample transfer module, and an enzyme storage module; the reagent disc is provided with a shaft hole in the center, and is provided with a reagent position and an enzyme storage position; the reagent position is used for storing a reagent bottle; the liquid sample transfer module comprises a mechanical arm and a liquid adding needle; the liquid adding needle is installed on the mechanical arm and used for extracting and releasing a liquid sample; the mechanical arm is installed through the shaft hole of the reagent disc, so that the liquid adding needle is located above the reagent disc; the enzyme storage module comprises an enzyme storage bottle and a refrigeration sheet; the enzyme storage bottle is arranged at the enzyme storage position of the reagent disc; and the refrigeration sheet is arranged towards the enzyme storage bottle and provides a cold source for the enzyme storage bottle; the mechanical arm drives the liquid adding needle to rotate, so that the liquid adding needle reaches the reagent position and the enzyme storage position.

[0007] In an optional embodiment, the enzyme storage module further comprises a storage box and a mounting block; the enzyme storage position is a circular through hole; the storage box is arranged below the reagent disc and is internally provided with a cavity; one end of the storage box towards the reagent disc is provided with a circular limiting part; the cavity in the storage box is used for placing the enzyme storage bottle, and the circular limiting part is clamped at the enzyme storage position to limit the upper end of the enzyme storage bottle; a first side wall of the storage box is provided with a cold source window; and the refrigeration sheet is installed at the cold source window through the mounting block, so that the refrigeration sheet provides a cold source for the enzyme storage bottle in the storage box.

[0008] In an optional embodiment, the cavity in the storage box is further provided with a heat dissipation sleeve; the heat dissipation sleeve is in contact with the refrigeration sheet and is used for placing the enzyme storage bottle and is made of an aluminum alloy material.

[0009] In an optional embodiment, the bottom of the storage box is provided with a temperature measuring hole, and the bottom of the heat dissipation sleeve is provided with a temperature measuring cavity; the enzyme storage module further comprises a temperature sensor; the temperature sensor passes through the temperature measuring hole and enters the temperature measuring cavity to detect the temperature of the heat dissipation sleeve.

[0010] In an optional embodiment, a second side wall of the storage box is provided with a detection window; a side wall of the heat dissipation sleeve is provided with a detection position corresponding to the detection window; the enzyme storage module further comprises an in-position detector; the in-position detector is installed below the reagent disc; and the in-position detector performs in-position detection on the enzyme storage bottle in the heat dissipation sleeve through the detection window and the detection position.

[0011] In an optional embodiment, an inner side wall of the circular limiting part is provided with a limiting groove; the in-position detector is a mechanical detector and comprises a moving contact and a stationary contact; the moving contact extends into the heat dissipation sleeve through the silica gel sleeve, is pressed by the enzyme storage bottle, and is in contact with the stationary contact to achieve in-position detection.

[0012] In an optional embodiment, the enzyme storage module further includes a heat sink mounted toward the cooling chip.

[0013] This utility model also provides a pesticide residue detection device, including the reagent storage and transfer device for pesticide residue detection described in the above embodiments.

[0014] The reagent storage and transfer device and pesticide residue detection equipment of this utility model for pesticide residue detection have the following beneficial effects:

[0015] Compared to traditional enzyme storage solutions, the technical solution in this embodiment combines the enzyme storage module with the reagent storage module, eliminating the need for separate enzyme storage modules independent of the pesticide residue detection equipment, thus improving portability for field testing. Simultaneously, the enzyme storage bottle and multiple reagent bottles are placed on a reagent tray, allowing for the extraction and addition of reagents and enzymes using the same injection needle, eliminating the need for a dedicated enzyme extraction needle and avoiding detection errors caused by manual operation. Compared to manual operation, automated enzyme extraction and addition to sample bottles is faster, thereby improving the efficiency of pesticide residue detection.

[0016] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit the present invention.

[0017] To better understand and implement this invention, the following detailed description is provided in conjunction with the accompanying drawings. Attached Figure Description

[0018] Figure 1 A perspective view of the pesticide residue detection equipment provided in the embodiments of this utility model;

[0019] Figure 2 A schematic diagram of the structure of a reagent storage and transfer device for pesticide residue detection provided in an embodiment of this utility model;

[0020] Figure 3 This is a schematic diagram of the structure of the enzyme storage module provided in an embodiment of the present invention;

[0021] Figure 4 A cross-sectional view of an enzyme storage module provided in an embodiment of this utility model;

[0022] Figure 5 A schematic diagram of the structure of the storage box provided in an embodiment of this utility model;

[0023] Figure 6 This is a schematic diagram of the installation of the heat sink provided in an embodiment of the present utility model.

[0024] Icon labels:

[0025] 1. Housing; 11. Frame; 12. Base; 211. Sample tray; 2111. Sample position; 22. Reagent storage module; 221. Reagent tray; 2211. Reagent position; 2213. Enzyme storage position; 2214. Cleaning position; 23. Liquid sample transfer module; 231. Robotic arm; 232. Liquid dispensing needle; 233. Drive assembly; 51. Storage box; 511. Cavity; 512. Circular limiting part; 513. Cold source window; 514. Temperature measuring hole; 515. Detection window; 52. Enzyme storage bottle; 53. Cooling element; 54. Mounting block; 55. Temperature sensor; 56. Heat sink; 57. Heat dissipation sleeve; 571. Temperature measuring chamber; 572. Detection position; 58. Silicone sleeve; 59. In-situ detector; 591. Moving contact. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0027] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0028] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0029] This utility model discloses a reagent storage and transfer device and a pesticide residue detection device for pesticide residue detection. The pesticide residue detection device is suitable for pesticide residue detection in diverse scenarios such as agricultural product quality supervision and inspection, health and epidemic prevention, environmental protection, industrial and commercial management, and vegetable wholesale markets. It employs an automated method to realize the steps of liquid sample transfer, buffer injection, liquid sample mixing, enzyme addition, chromogenic agent addition, substrate addition, and quantitative detection of pesticide residues at each stage. This pesticide residue detection device is suitable for the detection of organophosphates, carbamates, and other pesticide residues.

[0030] like Figure 1As shown, the pesticide residue testing equipment includes: a housing 1, and a reagent storage and transfer device for pesticide residue testing installed inside the housing 1. To improve the transfer convenience, ease of use, and environmental adaptability of the pesticide residue testing equipment, the housing 1 includes a top cover, a frame 11, and a base 12, which are installed together from top to bottom to form the housing 1.

[0031] like Figures 1-4 As shown, the reagent storage and transfer device for pesticide residue detection includes: a reagent storage module 22, a liquid sample transfer module 23, and an enzyme storage module.

[0032] The reagent storage module 22 includes a reagent tray 221; the reagent tray 221 has a central axial hole, and is provided with reagent positions 2211, cleaning positions 2212, and enzyme storage positions 2213. Reagent positions 2211, cleaning positions 2212, and enzyme storage positions 2213 are all circular through holes. Reagent positions 2211 are used to store reagent bottles, and enzyme storage positions 2213 are used to place enzyme storage bottles.

[0033] The liquid sample transfer module 23 includes a robotic arm 231 and a liquid dispensing needle 232; the liquid dispensing needle 232 is mounted on the robotic arm 231 and is used to extract and release liquid samples; the robotic arm 231 is mounted through the shaft hole of the reagent tray 221 so that the liquid dispensing needle 232 is located above the reagent tray, that is, the liquid dispensing needle 232 can be located above the reagent position 2211 and the enzyme storage position 2213.

[0034] The enzyme storage module includes an enzyme storage bottle 52 and a cooling plate 53. The enzyme storage bottle 52 is located at the enzyme storage position 2213 of the reagent storage module 22. The cooling plate 53 is positioned facing the enzyme storage bottle 52 to provide a cooling source for the enzyme storage bottle 52.

[0035] The robotic arm 231 drives the dispensing needle 232 to rotate, so that the dispensing needle 232 reaches the reagent position 2211 and above the enzyme storage position 2213; the robotic arm 231 drives the dispensing needle 232 to descend into the reagent bottle to extract the reagent, and the robotic arm 231 drives the dispensing needle 232 to descend into the enzyme storage bottle 52 to extract the enzyme, thereby realizing the automated extraction of reagents and enzymes and avoiding operational errors caused by manual enzyme addition.

[0036] Optionally, the robotic arm 231 is driven to rotate by a drive assembly 233. The drive assembly 233 is installed below the reagent tray 221 and connected to the second end of the robotic arm 231. The drive assembly 233 is used to drive the robotic arm 231 to lift or rotate, thereby driving the dispensing needle 232 to lift or rotate. The robotic arm 231 drives the dispensing needle 232 to rotate around the shaft hole of the reagent tray 221 with the horizontal distance between the shaft hole and the dispensing needle 232 as the radius. That is, the dispensing needle 232 moves along the circumference of a preset circle. Alternatively, the preset circle is centered on the shaft hole with the distance between the shaft hole and the center of the reagent position or the center of the enzyme storage position as the radius. The movement of the dispensing needle 232 along the circumference of the preset circle enables the dispensing of reagents from reagent bottles and the dispensing of enzymes from enzyme storage bottles.

[0037] Compared to traditional enzyme storage solutions, the technical solution in this embodiment integrates the enzyme storage module and reagent storage module onto a portable pesticide residue detection device, eliminating the need for separate enzyme storage equipment and improving portability for field testing. Furthermore, since the enzyme storage bottle and multiple reagent bottles are all located on a reagent tray, reagents and enzymes can be added using the same dispensing needle, eliminating the need for a dedicated enzyme extraction needle and avoiding detection errors caused by manual operation, thus improving detection accuracy. Compared to manual operation, automatic enzyme extraction and addition to sample bottles is faster, thereby improving pesticide residue detection efficiency.

[0038] In a preferred embodiment, the cooling chip 53 is a semiconductor cooling chip, which enables the enzyme to be stored in a low-temperature environment, ensuring the enzyme's activity. The enzyme storage module also includes a storage box 51 and a mounting block 54; the storage box 51 is located below the reagent tray 221, specifically fixed to the bottom of the reagent tray 221 by screws, and has an internal cavity 511; a circular limiting part 512 is provided at the end of the storage box 51 facing the reagent tray 221; during installation, the circular limiting part 512 is engaged with the enzyme storage position 2213 of the reagent tray 221. The cavity 511 in the storage box 51 is used to place the enzyme storage bottle 52, and the circular limiting part 512 limits the upper end of the enzyme storage bottle 52, preventing the enzyme storage bottle 52 from shaking during carrying and detection, thus improving the overall reliability of the device.

[0039] A cold source window 513 is provided on the first side wall of the storage box 51; a semiconductor cooling chip 53 is installed at the cold source window 513 by a mounting block 54. The semiconductor cooling chip 53 provides a cold source for the enzyme storage bottle 52 in the storage box 51 to ensure the low-temperature storage of the enzyme.

[0040] In a preferred embodiment, a heat dissipation sleeve 57 is further disposed within the cavity 511 of the storage box 51; the heat dissipation sleeve 57 is used to place the enzyme storage bottle 52. The heat dissipation sleeve 57 is in contact with the semiconductor cooling chip 53 and encloses the enzyme storage bottle 52, effectively conducting and dissipating heat from the enzyme storage bottle 52 to ensure low-temperature storage of the enzyme. The heat dissipation sleeve 57 is made of aluminum alloy material, further ensuring low-temperature storage of the enzyme.

[0041] In a preferred embodiment, the storage box 51 is provided with a temperature measuring hole 514 at the bottom, and the heat dissipation sleeve 57 is provided with a temperature measuring cavity 571 at the bottom; the enzyme storage module also includes a temperature sensor 55, which passes through the temperature measuring hole 514 and enters the temperature measuring cavity 571 to detect the temperature of the heat dissipation sleeve 57, so as to adjust the cooling temperature of the semiconductor cooling chip 53, so that the enzyme storage bottle is always in a constant temperature environment during portable storage.

[0042] A detection window 515 is provided on the second side wall of the storage box 51; a detection position 572 is provided on the side wall of the heat dissipation sleeve 57, corresponding to the detection window 515; the enzyme storage module also includes an in-situ detector 59, which is installed below the reagent tray 221. The in-situ detector 59 performs in-situ detection on the enzyme storage bottle 52 inside the heat dissipation sleeve 57 through the detection window 515 and the detection position 572. Before the pesticide residue detection begins, in-situ detection of reagents or enzymes is performed by the in-situ detector 59 to ensure the smooth progress of the detection.

[0043] A limiting groove 516 is provided on the inner side wall of the circular limiting part 512. The limiting groove 516 is used to place the silicone sleeve 58, so that the various components of the enzyme storage module are installed in close fit.

[0044] Optionally, the in-situ detector 59 is a mechanical detector, which includes a moving contact 591 and a stationary contact. The moving contact 591 extends through the silicone sleeve 58 into the heat dissipation sleeve 57, is squeezed by the enzyme storage bottle 52, and comes into contact with the stationary contact to achieve in-situ detection. Optionally, the in-situ detector can also be an electronic detector, such as a photoelectric sensor.

[0045] The enzyme storage module also includes a heat sink 56, which is mounted toward the semiconductor cooling chip 53 to regulate the ambient temperature of the enzyme storage bottle and ensure enzyme activity.

[0046] Optionally, to facilitate the extraction of reagents and enzymes using the same dispensing needle, the structure of the reagent tray and sample tray was cleverly designed. Specifically, for example... Figure 1 Figure 2As shown, the reagent tray 221 is crescent-shaped, and the sample tray 211 is a circular turntable. Multiple sample positions 2111 are arranged in a ring on the sample tray 211, used to hold sample vials. The reagent tray 221 and sample tray 211 are arranged side-by-side; the reagent tray 221 is fixed, while the sample tray 211 is rotatable. Multiple reagent positions 2211 are arranged in an arc shape on the side of the reagent tray 221 away from the sample tray 211, with each reagent position 2211 equidistant along the arc, making it easier for the dispensing needle 232 to be controlled and transferred to each reagent position 2211. Optionally, the reagent tray 221 has four reagent positions 2211, one enzyme storage position 2213, and one washing position 2214. The reagent tray 221 has an arc-shaped portion concave towards its center on the side near the sample tray 211. The outer edge of the sample tray 211 mates with the arc-shaped portion of the reagent tray 221, with a slight gap between them, allowing the sample tray 211 to rotate freely. This allows the robotic arm 231 to rotate and move the dispensing needle 232 to various sample vials, cuvettes, and reagent bottles to aspirate or release liquid samples. Optionally, a decorative sticker is affixed to the upper surface of the reagent tray 221 facing the cover, indicating the reagent slot number 2211, the cleaning tank label, and / or the enzyme storage label.

[0047] When the robotic arm 231 rotates the dispensing needle 232 on the reagent tray 221, the dispensing needle 232 reaches each reagent position 2211 to draw reagents. When the robotic arm 231 rotates the dispensing needle 232 to a first preset position outside the reagent tray 221, the sample tray 211 is driven to rotate, causing the sample vials on the sample positions to move sequentially below the dispensing needle 232, realizing liquid sample injection or extraction. When the robotic arm 231 rotates the dispensing needle 232 to a second preset position outside the reagent tray 221, the sample tray 211 is driven to rotate, causing the reaction tray under the sample tray 211 to rotate, causing the cuvettes on the cuvette positions to move sequentially below the dispensing needle 232, realizing liquid sample injection or extraction. Each reagent position 2211, enzyme storage position 2213, washing position 2214, the first preset position, and the second preset position are all on the circumference of the same preset circle.

[0048] The outer edge of the sample tray 211 is tightly fitted with the arc-shaped part of the reagent tray 221, which can reduce the radius of rotation of the robotic arm 231, reduce the platform area, thereby reducing the volume of the box, saving space, making it easy to carry and transport, and reducing the requirements for the testing environment.

[0049] Compared to traditional enzyme storage solutions, the technical solution in this embodiment integrates the enzyme storage module and reagent storage module onto a portable pesticide residue detection device, eliminating the need for a separate enzyme storage module and improving portability for field testing. Furthermore, with the enzyme storage bottle and multiple reagent bottles mounted on a reagent tray, reagents and enzymes can be added using a single dispensing needle, eliminating the need for a dedicated enzyme extraction needle and avoiding detection errors caused by manual operation, thus improving detection accuracy. Compared to manual operation, automated enzyme extraction and addition to sample bottles is faster, thereby improving pesticide residue detection efficiency.

[0050] Another embodiment of this utility model discloses a pesticide residue detection device, including the reagent storage and transfer device for pesticide residue detection as described in the above embodiment. For the contents not disclosed in this embodiment, please refer to the contents described in the above embodiment.

[0051] Note that the above description is merely a preferred embodiment of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and various obvious changes, readjustments, and substitutions can be made without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the concept of the present invention. The scope of the present invention is determined by the scope of the appended claims.

Claims

1. A reagent storage and transfer device for pesticide residue detection, characterized in that, include: Reagent storage module, liquid sample transfer module, and enzyme storage module; The reagent storage module includes a reagent tray; the reagent tray has a central shaft hole, and the reagent tray has reagent positions and enzyme storage positions, the reagent positions being used to store reagent bottles; The liquid sample transfer module includes a robotic arm and a dispensing needle; the dispensing needle is mounted on the robotic arm for extracting and releasing liquid samples; the robotic arm is installed through the shaft hole of the reagent tray, so that the dispensing needle is positioned above the reagent tray; The enzyme storage module includes an enzyme storage bottle and a cooling plate. The enzyme storage bottle is located at the enzyme storage position of the reagent tray, and the cooling plate is positioned towards the enzyme storage bottle to provide a cooling source for the enzyme storage bottle. The robotic arm drives the dispensing needle to rotate, so that the dispensing needle reaches the reagent position and the enzyme storage position.

2. The reagent storage and transfer device for pesticide residue detection according to claim 1, characterized in that, The enzyme storage module further includes a storage box and a mounting block; the enzyme storage position is a circular through hole; The storage box is located below the reagent tray and has an internal cavity. The storage box has a circular limiting part at one end facing the reagent tray; the cavity inside the storage box is used to place the enzyme storage bottle, and the circular limiting part is locked at the enzyme storage position to limit the upper end of the enzyme storage bottle; The storage box has a cold source window on its first side wall; the cooling chip is installed at the cold source window via the mounting block, and the cooling chip provides a cold source for the enzyme storage bottles inside the storage box.

3. The reagent storage and transfer device for pesticide residue detection according to claim 2, characterized in that, The storage box is also equipped with a heat dissipation sleeve inside its cavity; the heat dissipation sleeve is in contact with the cooling chip and is used to place the enzyme storage bottle, which is made of aluminum alloy.

4. The reagent storage and transfer device for pesticide residue detection according to claim 3, characterized in that, The storage box has a temperature measuring hole at the bottom, and the heat dissipation sleeve has a temperature measuring cavity at the bottom. The enzyme storage module also includes a temperature sensor that passes through the temperature measuring hole and enters the temperature measuring chamber to detect the temperature of the heat sink.

5. The reagent storage and transfer device for pesticide residue detection according to claim 3, characterized in that, A detection window is provided on the second side wall of the storage box; a detection position is provided on the side wall of the heat dissipation sleeve, and the detection position corresponds to the detection window; The enzyme storage module also includes an in-situ detector, which is installed below the reagent tray. The in-situ detector performs in-situ detection on the enzyme storage bottle inside the heat sink through the detection window and the detection position.

6. The reagent storage and transfer device for pesticide residue detection according to claim 5, characterized in that, A limiting groove is provided on the inner sidewall of the circular limiting part, and the limiting groove is used to place the silicone sleeve. The in-situ detector is a mechanical detector, which includes a moving contact and a stationary contact. The moving contact extends through the silicone sleeve into the heat dissipation sleeve and is squeezed by the enzyme storage bottle to contact the stationary contact, thereby realizing in-situ detection.

7. The reagent storage and transfer device for pesticide residue detection according to claim 2, characterized in that, The enzyme storage module also includes a heat sink, which is mounted toward the cooling chip.

8. The reagent storage and transfer device for pesticide residue detection according to claim 1, characterized in that, The cooling chip is a semiconductor cooling chip.

9. A pesticide residue detection device, characterized in that, Includes the reagent storage and transfer device for pesticide residue detection as described in any one of claims 1 to 8.

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