Microscopic imaging device
By integrating a multi-channel LED light source module, a multi-channel excitation plate, and a dichroic mirror into a microscopic imaging device, the problem of requiring manual or electronic switching of the light source and filter in existing technologies has been solved, achieving a simple and compact fluorescence imaging effect.
Patent Information
- Application Number
- CN202520112604.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-17
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2035-01-17
AI Technical Summary
Existing fluorescence microscopy devices require manual or electronic switching of light sources and filters to achieve observation of multiple fluorescence channels, which is inconvenient to operate.
It adopts an integrated design of multi-channel LED light source module, multi-channel excitation plate, dichroic mirror and multi-channel emitting plate. The light source module provides light of multiple wavelengths, and the multi-channel excitation plate and dichroic mirror realize automatic selection of excitation light, avoiding manual or electronic control switching.
It achieves a high degree of integration and ease of operation of the fluorescence imaging device, enabling fluorescence imaging observation under various fluorescent reagents without switching fluorescence components, thus improving operational efficiency and device compactness.
Smart Images
Figure CN223711916U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present disclosure relates to an optical device, in particular to a microscopic imaging device. BACKGROUND
[0002] At present, the microscopic imaging device is widely used in the fields of biological and medical research, which can perform real-time fluorescence observation and measurement on cells, sections and stained samples.
[0003] The fluorescence microscopic imaging technology can perform imaging in multiple ways, such as two-dimensional, three-dimensional, time sequence and the like. Moreover, the fluorescence microscopic imaging technology can observe the specific structure and physiological function of microorganism systems such as living cells, animals and bacteria in real time. Therefore, the fluorescence microscopic imaging technology has been widely applied in biomedical, medical diagnosis and drug development.
[0004] The basic principle of the fluorescence microscopic imaging technology is to use excitation light of a certain wavelength to excite the sample to generate fluorescence of a certain wavelength, and to perform qualitative, positioning and quantitative observation and detection on the structure or constituent components of the sample. The fluorescence microscope in the prior art generally includes a light source and a filter, which need to be switched by electric control or manually to realize observation of multiple fluorescence channels, which is inconvenient to operate. CONTENT OF THE INVENTION
[0005] The present disclosure provides a microscopic imaging device.
[0006] According to one aspect of the present disclosure, a microscopic imaging device is provided, which comprises:
[0007] a light source module for emitting light of multiple wavebands;
[0008] a multi-channel excitation sheet for selectively passing light of at least two wavebands of the multiple wavebands;
[0009] a dichroic mirror for reflecting light passing through the multi-channel excitation sheet to a first imaging lens and providing the light to an observation sample through the first imaging lens; light emitted by the observation sample is transmitted through the dichroic mirror after passing through the first imaging lens;
[0010] an emission sheet for filtering the light transmitted by the dichroic mirror; and
[0011] an imaging module for imaging the light filtered by the emission sheet.
[0012] The microscopic imaging device according to at least one embodiment of the present disclosure further comprises:
[0013] A condenser is arranged between the light source module and the multi-channel excitation sheet, and is used to shape the light emitted by the light source module.
[0014] According to the microscopic imaging device of at least one embodiment of the present disclosure, the light source module comprises a plurality of LED chips, wherein the plurality of LED chips at least comprises LED chips capable of emitting light of 385nm, 475nm, 545nm, 525nm, 585nm, 630nm and 680nm.
[0015] According to the microscopic imaging device of at least one embodiment of the present disclosure, each of the plurality of LED chips can be independently controlled.
[0016] According to the microscopic imaging device of at least one embodiment of the present disclosure, the multi-channel excitation sheet is used to selectively pass red light, green light, blue light and ultraviolet light.
[0017] According to the microscopic imaging device of at least one embodiment of the present disclosure, the dichroic mirror is a multi-channel dichroic mirror.
[0018] According to the microscopic imaging device of at least one embodiment of the present disclosure, the emission sheet is a multi-channel emission sheet.
[0019] According to the microscopic imaging device of at least one embodiment of the present disclosure, further comprising:
[0020] A second imaging lens is arranged between the emission sheet and an imaging module.
[0021] According to the microscopic imaging device of at least one embodiment of the present disclosure, the second imaging lens comprises at least two lenses.
[0022] According to the microscopic imaging device of at least one embodiment of the present disclosure, the imaging module is a CMOS image sensor.
[0023] The microscopic imaging device of the present disclosure can be formed into a highly integrated structure, and through the multiple wave bands of light provided by the light source module and the fall illumination method, uniform illumination of the entire field of view can be achieved, avoiding the electric control or manual control of the light source module. Through the arrangement of the multi-channel excitation sheet and the multi-channel dichroic mirror, manual or electric control switching of the fluorescent component or the light source is avoided. Moreover, the microscopic imaging device of the present disclosure is easy to operate and compact in structure, and can realize fluorescent imaging observation under one or more fluorescent reagents without switching the fluorescent component. BRIEF DESCRIPTION OF DRAWINGS
[0024] The accompanying drawings, which are included to provide a further understanding of the present disclosure and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the present disclosure and together with the description serve to explain the principles of the present disclosure.
[0025] Figure 1 is a structural schematic diagram of a microscopic imaging device according to an embodiment of the present disclosure.
[0026] Figure 2 is a structural schematic diagram of a light source module according to an embodiment of the present disclosure.
[0027] The reference signs in the drawings are specifically as follows:
[0028] 100 light source module
[0029] 110 substrate
[0030] 120 LED chip
[0031] 200 multi-channel excitation sheet
[0032] 300 dichroic mirror
[0033] 400 first imaging lens
[0034] 500 emission sheet
[0035] 600 imaging module
[0036] 700 condenser lens
[0037] 800 second imaging lens. DETAILED DESCRIPTION
[0038] The present disclosure will be described in further detail below with reference to the drawings and embodiments. It can be understood that the specific embodiments described herein are only intended to explain the related content, and not to limit the present disclosure. In addition, it should be noted that only parts related to the present disclosure are shown in the drawings for ease of description.
[0039] It should be noted that the embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict. The technical solutions of the present disclosure will be described in detail below with reference to the drawings and in combination with the embodiments.
[0040] Unless otherwise specified, the exemplary embodiments / instances shown will be understood to provide exemplary features of various details that can implement the technical concepts of the present disclosure in practice. Therefore, unless otherwise specified, the features of various embodiments / instances can be additionally combined, separated, interchanged and / or rearranged without departing from the technical concepts of the present disclosure.
[0041] The use of cross-hatching and / or shading in the drawings is generally used to illustrate the boundaries and / or transitions between adjacent portions of a part. As such, unless specified, the presence or absence of cross-hatching and / or shading is not intended to convey or imply any preference or requirement for specific material, material properties, dimensions, proportions, commonality of the illustrated parts between the parts, and / or any other characteristic, attribute, property, etc. of the parts. Moreover, in the drawings, the size and relative sizes of parts can be exaggerated for clarity and / or descriptive purposes. When exemplary embodiments can be practiced differently, a specific sequence of processes can be performed in a different order than described. For example, two consecutively described processes can be performed substantially simultaneously or in the opposite order to that described. Moreover, like reference numerals can denote like parts throughout the specification.
[0042] When a part is referred to as being "on" or "over" another part, "connected to" or "coupled to" another part, it can be directly on, connected or coupled to the other part, or intervening parts can be present. However, when a part is referred to as being "directly on", "directly connected to", or "directly coupled to" another part, there are no intervening parts present. For this reason, the term "connected" can refer to physical, electrical, or other connections, with or without intervening parts.
[0043] For descriptive purposes, the disclosure can use spatial or relative terms, such as "below", "lower", "under", "downward", "below" "upward", "above", "higher", and "side" (e.g., as in "sidewall") to describe the relationship between one part and another part as the drawings suggest. The spatial or relative terms are intended to encompass different orientations of the device in use, operation, and / or manufacture in addition to the orientation depicted in the drawings. For example, if the device in the drawings is turned over, elements described as "below" or "under" other elements or features would then be oriented "above" the other elements or features. Thus, the exemplary term "below" can encompass both an orientation of above and below. Moreover, the device can be otherwise oriented (e.g., rotated 90 degrees or at other orientations) and the spatial or relative descriptors used herein interpreted accordingly.
[0044] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. As used herein, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. Furthermore, to the extent that the terms "comprising," "including," "containing," and / or "having" and variations thereof are used herein, such terms are intended to be inclusive in a manner similar to the term "comprising" as that term is interpreted when employed as a transitional term in a claim. It is also noted that the terms "substantial," "approximately," and other similar terms, as used herein, are used in their normal, ordinary sense and are not used in an absolute sense.
[0045] Figure 1 is a structural schematic diagram of a microscopic imaging device according to an embodiment of the present disclosure.
[0046] As shown in Figure 1 , the microscopic imaging device of the present disclosure, in use, does not need to switch fluorescent components, i.e. can realize fluorescent observation under one or more fluorescent reagents, greatly simplifying the operation method of the fluorescent microscopic imaging device.
[0047] Specifically, as shown in Figure 1 , the microscopic imaging device can include a light source module 100, a multi-channel excitation sheet 200, a dichroic mirror 300, an emission sheet 500, and an imaging module 600, etc.
[0048] Figure 2 is a structural schematic diagram of a light source module according to an embodiment of the present disclosure.
[0049] As shown in Figure 2 , the light source module 100 of the present disclosure can include a substrate 110 and a plurality of LED chips 120 disposed on the substrate 110. These LED chips 120 can be arranged in an array on the substrate 110 and can be controlled individually and can be lit simultaneously, so that different wavelengths of light can be obtained by providing electrical energy to different LED chips 120.
[0050] In a preferred embodiment, the plurality of LED chips 120 at least includes LED chips 120 capable of emitting light of 385nm, 475nm (blue light), 545nm, 525nm (green light), 585nm, 630nm (red light), and 680nm. In other words, the light source module 100 of the present disclosure can at least emit blue light, red light, green light, and ultraviolet light, etc.
[0051] As shown in Figure 1As shown, the condenser 700 of the present disclosure is arranged between the light source module 100 and the multi-channel excitation sheet 200, and is used to shape the light emitted by the light source module 100. Accordingly, the light shaped by the condenser 700 can be provided to the multi-channel excitation sheet 200.
[0052] The multi-channel excitation sheet 200 selectively passes at least two wavebands of light among a plurality of wavebands of light. In one specific embodiment, the multi-channel excitation sheet 200 is used to selectively pass red light, green light, blue light and ultraviolet light, that is, the multi-channel excitation sheet 200 of the present disclosure can be a four-channel excitation sheet 200. Moreover, in the microscopic imaging device of the present disclosure, the light required for imaging can be selectively passed by replacing different multi-channel excitation sheets 200.
[0053] The dichroic mirror 300 of the present disclosure is a multi-channel dichroic mirror. Specifically, the dichroic mirror 300 is used to reflect the light that has passed through the multi-channel excitation sheet 200 to the first imaging lens 400, and provide the light to the observation sample through the first imaging lens 400. At this time, the light provided to the observation sample can be referred to as excitation light, and after the excitation light is provided to the observation sample, the observation sample can be excited by the excitation light and generate emission light.
[0054] The light emitted by the observation sample is transmitted through the first imaging lens 400 and then transmitted through the dichroic mirror 300. Further, the light transmitted through the dichroic mirror 300 can be provided to the emission sheet 500.
[0055] In the present disclosure, the emission sheet 500 is a multi-channel emission sheet, and the emission sheet 500 can be used to filter the light transmitted by the dichroic mirror 300. In one preferred embodiment, the emission sheet 500 can also be a four-channel emission sheet.
[0056] The light that has passed through the emission sheet 500 can be provided to the second imaging lens 800, and the second imaging lens 800 is arranged between the emission sheet 500 and the imaging module 600. The imaging module 600 is used to image the light filtered by the emission sheet 500.
[0057] The second imaging lens 800 of the present disclosure includes at least two lenses. In one preferred embodiment, the two lenses can be convex lenses, and the distance between the two lenses can be adjusted so that the imaging module 600 can generate a clear image.
[0058] In some embodiments, the imaging module 600 is a CMOS image sensor.
[0059] Based on the above structure, the microscopic imaging device of the present disclosure can be formed into a highly integrated structure, and through the multiple waveband lights provided by the light source module 100 and the drop illumination method, uniform illumination of the entire field of view can be achieved, avoiding the electrical control or manual control of the light source module. Through the multi-channel excitation sheet 200 and the multi-channel dichroic mirror, manual or electrical control switching of the fluorescence components or light sources is avoided. Moreover, the microscopic imaging device of the present disclosure is easy to operate and compact in structure, and can achieve fluorescence imaging observation under one or more fluorescent reagents without switching the fluorescence components.
[0060] The microscopic imaging device of the present disclosure is also applicable to other special waveband fluorescence detection, and can also be used as a basic imaging module for other complex microscopes, cell imagers and other devices such as automatic focusing, which is reasonable in layout and has practicality.
[0061] Taking a four-channel fluorescence microscopic imaging device as an example, the light source module 100 directly emits a mixed color spectrum containing 385nm, 475nm, 545nm, 525nm, 585nm, 630nm and 680nm after being powered on, and the light source is shaped through the condenser lens 700. The shaped light beam is filtered through the multi-channel excitation sheet 200 to obtain four colors of excitation light, which is reflected to the first imaging lens through the multi-channel dichroic mirror, falls on the observed sample, and excites four colors of emission light. The emission light passes through the first imaging lens, the multi-channel dichroic mirror and the multi-channel emission sheet in turn, and is imaged by the second imaging lens, and finally image acquisition is realized on the COMS image sensor.
[0062] Taking a three-channel fluorescence microscopic imaging device as an example, according to the design requirements of the excitation light waveband, three LED chips are selectively driven to emit light, and a mixed color spectrum containing three required excitation light spectra is emitted. The light source is shaped through the condenser lens 700. The shaped light beam is filtered through the multi-channel excitation sheet 200 to obtain three colors of excitation light, which is reflected to the first imaging lens through the multi-channel dichroic mirror, falls on the observed sample, and excites three colors of emission light. The emission light passes through the first imaging lens, the multi-channel dichroic mirror and the multi-channel emission sheet in turn, and is imaged by the second imaging lens, and finally image acquisition is realized on the COMS image sensor.
[0063] Taking a two-channel fluorescence microscopic imaging device as an example, according to the excitation light wavelength band required by the design, the selective light emission of two LED chips is driven and controlled, the mixed color spectrum containing the required two kinds of excitation light spectrum is emitted, the light source is shaped through the condenser lens 700, the shaped light beam is filtered through the multi-channel excitation sheet 200 to obtain the required two colors of excitation light, the two colors of excitation light are reflected to the first imaging lens through the multi-channel dichroic mirror, fall on the observed sample, and excite another two colors of emission light. The emission light is sequentially imaged by the second imaging lens after passing through the first imaging lens, the multi-channel dichroic mirror and the multi-channel emission sheet, and finally the image acquisition is realized on the COMS image sensor.
[0064] Taking a two-channel fluorescence microscopic imaging device as an example, according to the excitation light wavelength band required by the design, the selective light emission of two LED chips is driven and controlled, the mixed color spectrum containing the required two kinds of excitation light spectrum is emitted, the light source is shaped through the condenser lens 700, the shaped light beam is filtered through the multi-channel excitation sheet 200 to obtain the required two colors of excitation light, the two colors of excitation light are reflected to the first imaging lens through the multi-channel dichroic mirror, fall on the observed sample, and excite another two colors of emission light. The emission light is sequentially imaged by the second imaging lens after passing through the first imaging lens, the multi-channel dichroic mirror and the multi-channel emission sheet, and finally the image acquisition is realized on the COMS image sensor.
[0065] In the description of the present specification, the description of the terms "one embodiment / way", "some embodiments / ways", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment / way or example are included in at least one embodiment / way or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment / way or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments / ways or examples. In addition, the person skilled in the art can combine and combine the different embodiments / ways or examples described in the present specification and the features of the different embodiments / ways or examples without contradiction.
[0066] In addition, the terms "first", "second" are only for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "multiple" is at least two, for example, two, three, etc., unless otherwise specifically limited.
[0067] Those skilled in the art should understand that the above embodiments are only for clearly illustrating the present disclosure, and are not intended to limit the scope of the present disclosure. Based on the above disclosure, other changes or modifications can also be made by those skilled in the art, and these changes or modifications are still within the scope of the present disclosure.
Claims
1. A microscopic imaging device, characterized in that, include: A light source module, which is used to emit light in multiple wavelength bands; A multi-channel excitation plate that selectively transmits light from at least two of a plurality of wavelength bands; A dichroic mirror is used to reflect light passing through a multi-channel excitation plate to a first imaging lens, and then provide the light to the observation sample through the first imaging lens; the light emitted by the observation sample passes through the first imaging lens and is transmitted through the dichroic mirror. An emitting plate, wherein the emitting plate is used to filter the light transmitted by the dichroic mirror; as well as An imaging module is used to image the light after it has been filtered by the emitter.
2. The microscopic imaging device according to claim 1, characterized in that, Also includes: A condenser lens is disposed between the light source module and the multi-channel excitation plate to shape the light emitted by the light source module.
3. The microscopic imaging device according to claim 1, characterized in that, The light source module includes multiple LED chips, wherein the multiple LED chips include at least LED chips capable of emitting light at 385nm, 475nm, 545nm, 525nm, 585nm, 630nm and 680nm.
4. The microscopic imaging device according to claim 3, characterized in that, Each of the plurality of LED chips can be controlled independently.
5. The microscopic imaging device according to claim 3, characterized in that, The multi-channel excitation plate is used to selectively transmit red, green, blue, and ultraviolet light.
6. The microscopic imaging apparatus according to claim 1, characterized in that, The dichroic mirror is a multi-channel dichroic mirror.
7. The microscopic imaging apparatus according to claim 1, characterized in that, The transmitter is a multi-channel transmitter.
8. The microscopic imaging apparatus according to claim 1, characterized in that, Also includes: The second imaging lens is disposed between the emitting sheet and the imaging module.
9. The microscopic imaging apparatus according to claim 8, characterized in that, The second imaging lens includes at least two lenses.
10. The microscopic imaging apparatus according to claim 1, characterized in that, The imaging module is a CMOS image sensor.