Microfluidic device

Abstract

The utility model relates to the technical field of microfluidics, and discloses a microfluidics device. A microfluidic main body of the microfluidic device is provided with a first liquid flow channel and a second liquid flow channel, the first liquid flow channel comprises a first liquid injection port, a mixing communication port and a first pressure equalizing port which are communicated in sequence, and the second liquid flow channel comprises a second liquid injection port, a fusion cavity, a reaction cavity and a second pressure equalizing port which are communicated in sequence; the control unit comprises a micro-flow pump, a flow meter, a liquid drop generator and a temperature control chip which are in communication connection, the micro-flow pump and the flow meter are both arranged between the second liquid injection opening and the fusion cavity and used for pumping a preset amount of second solution to the fusion cavity, and the liquid drop generator is arranged at the cavity bottom of the reaction cavity and communicated with the mixing communication opening; the dispersing device is used for dispersing a first solution to be mixed with a second solution in the fusion cavity to form a mixed solution; the temperature control chip is arranged in the reaction cavity and used for providing a preset temperature for the mixed solution in the reaction cavity. The micro-fluidic device disclosed by the utility model is higher in integration level and has more functions at the same time.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic technology field especially, relates to a micro -fluidic device. BACKGROUND

[0002] Micro -fluidic is the important tool of chemical, biology, medical to micro -fluid detection. Current micro -fluid detection system all just make the structure size of flow channel small, but power source, detection unit, temperature control unit etc. are external. Result in micro -fluidic chip integration degree is low, and detection equipment volume is bigger.

[0003] Therefore, the micro -fluidic device is urgently needed to solve the above -mentioned problems. UTILITY MODEL CONTENT

[0004] Based on above, the utility model discloses a purpose in at providing a kind of micro -fluidic device, integration degree is higher, more function simultaneously.

[0005] To achieve the above purpose, the utility model adopts following technical scheme:

[0006] Micro -fluidic device, comprising:

[0007] Micro -fluid main body is provided with first liquid flow channel and second liquid flow channel, the first liquid flow channel includes the first liquid injection port, the mixed communication port and the first pressure equalization port that are sequentially communicated, the second liquid flow channel includes the second liquid injection port, the fusion cavity, the reaction cavity and the second pressure equalization port that are sequentially communicated;

[0008] Control unit is set to the micro -fluid main body, and the control unit includes the micro -flow pump, flowmeter, droplet generator and temperature control chip that are communicated, the micro -flow pump and the flowmeter are all set between the second liquid injection port and the fusion cavity, for pumping the second solution of preset amount to the fusion cavity, the droplet generator is set to the chamber bottom of the reaction cavity, and is communicated with the mixed communication port, for dispersing first solution to mix with second solution in the fusion cavity and form mixed solution;The temperature control chip is set to the reaction cavity, for providing preset temperature for the mixed solution in the reaction cavity.

[0009] As a preferred scheme of micro -fluidic device, the micro -fluid main body includes seat body, the fusion cavity and the reaction cavity are all set to the seat body, and the seat body is also provided with the first liquid injection channel that is communicated with the first liquid injection port and the first pressure equalization channel that is communicated with the first pressure equalization port;The micro -fluid main body is also provided with base, and the base is set to the seat body near the chamber bottom of the fusion cavity, and the mixed communication port is set to the base, and the base is also provided with the first liquid injection communication port that is communicated with the first liquid injection channel and the first pressure equalization communication port that is communicated with the first pressure equalization channel, and the first liquid injection communication port and the first pressure equalization communication port are all communicated with the mixed communication port.

[0010] As a preferred solution of the microfluidic device, the base body comprises a bottom plate, the bottom plate is provided with a first flow channel groove and a second flow channel groove on the side away from the base, the reaction cavity and the second pressure equalization port are arranged on the bottom plate, one end of the first flow channel groove is in communication with the reaction cavity, the other end is provided with a fusion groove, the second flow channel groove is in communication with the fusion groove; the base body further comprises an upper cover, the upper cover is arranged on the side of the bottom plate away from the base, the first liquid injection port and the first pressure equalization port are arranged on the upper cover, the upper cover is provided with a second liquid injection port, and the second liquid injection port can be in communication with the second flow channel groove.

[0011] As a preferred solution of the microfluidic device, the base is provided with a communication groove, the communication groove is in communication with the second flow channel groove and the fusion cavity.

[0012] As a preferred solution of the microfluidic device, the side of the bottom plate close to the base is further provided with a flow detection groove, the flow detection groove is in communication with the second flow channel groove, and the detection end of the flow meter is arranged in the flow detection groove; and / or, the side of the bottom plate close to the base is further provided with a micro-pump mounting groove, the micro-pump mounting groove is arranged at the groove bottom of the second flow channel groove and is in communication with the second flow channel groove, and the micro-pump is arranged in the micro-pump mounting groove.

[0013] As a preferred solution of the microfluidic device, the bottom plate, the upper cover and the base are made of transparent plastic or glass; the bottom plate, the upper cover and the base are connected by bonding or bonding.

[0014] As a preferred solution of the microfluidic device, the microfluidic body further comprises a lower cover, the base is provided with a through groove on the side away from the base body, the through groove is in communication with the first liquid injection communication port, the first pressure equalization communication port and the mixing communication port, and the lower cover is arranged on the side of the base away from the base body.

[0015] As a preferred solution of the microfluidic device, the droplet generator comprises a droplet generation plate and a droplet generation line in communication with the droplet generation plate, the droplet generation plate is arranged on the cavity bottom of the fusion cavity and is in communication with the mixing communication port, and the droplet generation line is arranged in a U shape and is clamped on the side of the lower cover and the base away from each other.

[0016] As a preferred solution of the microfluidic device, the microfluidic body is further provided with a chip mounting groove, the chip mounting groove is arranged corresponding to the reaction cavity, and the temperature control chip is arranged in the chip mounting groove.

[0017] As a preferred solution of the microfluidic device, the microfluidic pump is arranged at the end of the flow meter away from the fusion cavity.

[0018] The microfluidic device has the advantages that:

[0019] The microfluidic device has the advantages that: BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments of the present application will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art according to the contents of the embodiments of the present application and the drawings without any creative labor.

[0021] Figure 1 is an exploded schematic view of the microfluidic device provided by the specific embodiment of the present application;

[0022] Figure 2 is a schematic view of one perspective of the bottom plate of the microfluidic device provided by the specific embodiment of the present application;

[0023] Figure 3 is a schematic view of another perspective of the bottom plate of the microfluidic device provided by the specific embodiment of the present application;

[0024] Figure 4 is a schematic view of another perspective of the base of the microfluidic device provided by the specific embodiment of the present application.

[0025] In the drawings:

[0026] 110, bottom plate; 111, first liquid injection channel; 112, first pressure equalization channel; 113, first flow channel groove; 1131, fusion groove; 114, second flow channel groove; 115, flow detection groove; 1151, first mounting groove; 116, micro-pump mounting groove; 1161, second mounting groove; 117, chip mounting groove; 118, reaction groove; 119, second pressure equalization port; 120, upper cover; 121, first liquid injection port; 122, first pressure equalization port; 123, second liquid injection port; 124, avoidance notch;

[0027] 200, base; 210, mixed communication port; 220, first liquid injection communication port; 230, first pressure equalization communication port; 240, communication groove; 250, through groove;

[0028] 310, micro-pump; 311, micro-pump wire; 320, flow meter; 321, detection end; 322, flow wire; 331, droplet generation plate; 332, droplet generation wire; 340, temperature control chip;

[0029] 410, lower cover; 420, sealing plate. DETAILED DESCRIPTION

[0030] The embodiments of the present application will be described in detail below, examples of the embodiments are shown in the drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0031] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application. In addition, the terms "first" and "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0032] Unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing" should be interpreted broadly. For example, they can refer to fixed connections or detachable connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and connections within two components or interactions between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0033] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0034] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.

[0035] like Figures 1-4 As shown, this embodiment provides a microfluidic device, which includes a microfluidic body and a control unit disposed on the microfluidic body. The microfluidic body is provided with a first liquid channel and a second liquid channel. The first liquid channel includes a first injection port 121, a mixing port 210, and a first equalization port 122 connected in sequence. The second liquid channel includes a second injection port 123, a fusion chamber, a reaction chamber, and a second equalization port 119 connected in sequence. The control unit includes a microfluidic pump 310, a flow meter 320, a droplet generator, and a temperature control chip 340 connected in communication. The microfluidic pump 310 and the flow meter 320 are both disposed between the second injection port 123 and the fusion chamber, and are used to pump a preset amount of second solution into the fusion chamber. The droplet generator is disposed at the bottom of the reaction chamber and is connected to the mixing port 210, and is used to disperse the first solution to mix with the second solution in the fusion chamber to form a mixed solution. The temperature control chip 340 is disposed in the reaction chamber and is used to provide a preset temperature for the mixed solution in the reaction chamber.

[0036] The control unit is arranged on the microfluidic body to realize different functions and improve the integration. Specifically, the microfluidic body is provided with a first liquid flow channel and a second liquid flow channel for flowing of the first liquid and the second liquid respectively. The liquid injection port is used for injection of the liquid, and the pressure equalization port is used for discharge of gas or liquid in the fluid channel to equalize the air pressure in the liquid flow channel. Before use, the second liquid is injected from the second liquid injection port 123 and fills the second liquid flow channel. During the injection process, the second pressure equalization port 119 discharges gas to make the injection more smooth. Then the first liquid is injected from the first liquid injection port 121, and the second pressure equalization port 119 discharges gas during the injection process. Then the first liquid injected from the first liquid injection port 121 enters the droplet generator through the mixing communication port 210, and is processed by the droplet generator, dispersed and mixed with the second solution in the fusion chamber to form a mixed solution. Next, the microfluidic pump 310 and the flow meter 320 work to pump a preset amount of second solution to the fusion chamber, so that the mixed solution in the fusion chamber enters the reaction chamber, and the second pressure equalization port 119 discharges the second liquid. After the mixed solution enters the reaction chamber, the temperature control chip 340 works to cool or heat the mixed solution in the reaction chamber to a preset temperature, thereby realizing detection of the liquid. The above microfluidic device has higher integration and more functions, and can be suitable for different use scenarios.

[0037] It can be understood that the microfluidic device is also connected with a power supply system and a control system. The power supply system is used to supply power to the control unit, and the control unit is in communication connection with the control system to operate the liquid according to a preset control program, thereby achieving a preset function. The control method of the control system and each control unit can be set according to the prior art, which is not limited here.

[0038] Exemplarily, the above microfluidic device can be used in dPCR (Digital PCR) detection. Accordingly, the first liquid is a reaction reagent, and the second liquid is a transport liquid such as fluorinated oil. The temperature control chip 340 needs to realize high and low temperature cycles. In other embodiments, the microfluidic device can also be used in other scenarios requiring temperature control and flow control, or special scenarios such as ships and space.

[0039] Specifically, the microfluidic body includes a seat body, the fusion cavity and the reaction cavity are arranged on the seat body, the seat body is further provided with a first liquid injection channel 111 in communication with the first liquid injection port 121 and a first pressure equalization channel 112 in communication with the first pressure equalization port 122; the microfluidic body is further provided with a base 200, the base 200 is arranged on one side of the seat body close to the cavity bottom of the fusion cavity, a mixed communication port 210 is arranged on the base 200, and correspondingly, the base 200 is further provided with a first liquid injection communication port 220 in communication with the first liquid injection channel 111 and a first pressure equalization communication port 230 in communication with the first pressure equalization channel 112, and the first liquid injection communication port 220 and the first pressure equalization communication port 230 are both in communication with the mixed communication port 210. Exemplarily, the droplet generator includes a droplet generation plate 331, and the droplet generation plate 331 is arranged on the cavity bottom of the fusion cavity to communicate the mixed communication port 210 and the fusion cavity. That is, the flow path of the first liquid is as follows: the first liquid injection port 121, the first liquid injection channel 111, the first liquid injection communication port 220, and then enters the droplet generator through the mixed communication port 210, and at the same time, the gas in the first liquid flow channel can be discharged from the microfluidic device through the first pressure equalization communication port 230, the first pressure equalization channel 112 and the first pressure equalization port 122. By arranging the base 200 for guiding the first liquid to the lower side of the fusion cavity, the second liquid dispersed into the fusion cavity after the first liquid is processed by the droplet generator is facilitated.

[0040] In the embodiment, the microfluidic body further includes a lower cover 410, and the base 200 is provided with a communication groove 240 on the side away from the seat body, the communication groove 240 is in communication with the first liquid injection communication port 220, the first pressure equalization communication port 230 and the mixed communication port 210, and the lower cover 410 is arranged on the side of the base 200 away from the seat body. By arranging the communication groove 240 for communicating the first liquid injection communication port 220, the first pressure equalization communication port 230 and the mixed communication port 210, and arranging the lower cover 410 to block the communication groove 240 to form a sealed liquid flow channel, the processing is facilitated. Exemplarily, the droplet generation plate 331 is provided with two liquid inlets, and correspondingly, the mixed communication port 210 is also provided with two.

[0041] Further, the droplet generator includes a droplet generation cable 332 in communication connection with the droplet generation plate 331, the droplet generation cable 332 is arranged in a U shape and is clamped on the side of the lower cover 410 and the base 200 away from each other, which can be used to fix the lower cover 410 and the base 200 or improve the connection reliability between the lower cover 410 and the base 200.

[0042] Exemplarily, the seat body comprises a bottom plate 110, the bottom plate 110 is provided with a first flow channel groove 113 and a second flow channel groove 114 on the side away from the base 200, the reaction cavity and the second pressure equalization port 119 are both arranged on the bottom plate 110, one end of the first flow channel groove 113 is communicated with the reaction cavity, the other end is provided with a fusion groove 1131, the second flow channel groove 114 is communicated with the fusion groove 1131; the seat body further comprises an upper cover 120, the upper cover 120 is arranged on the side of the bottom plate 110 away from the base 200 to form a fusion cavity with the fusion groove 1131. The upper cover 120 is provided with a second liquid injection port 123, the second liquid injection port 123 can be communicated with the second flow channel groove 114; the first liquid injection port 121 and the first pressure equalization port 122 are both arranged on the upper cover 120, and the first liquid injection channel 111 and the first pressure equalization channel 112 are arranged on the bottom plate 110.

[0043] In the embodiment, in order to realize the communication between the second flow channel groove 114 and the fusion groove 1131, the base 200 is provided with a through groove 250 on the side close to the bottom plate 110, and the second flow channel groove 114 and the fusion cavity are communicated through the through groove 250. It can be understood that the second liquid directly enters the fusion cavity through the through groove 250 and does not pass through the droplet generating plate 331.

[0044] Optionally, the bottom plate 110 is further provided with a flow detection groove 115 on the side close to the base 200, the flow detection groove 115 is communicated with the second flow channel groove 114, the detection end 321 of the flow meter 320 is arranged in the flow detection groove 115, and the liquid is metered by flowing through the detection end 321. At the same time, the bottom plate 110 is further provided with a micro-pump mounting groove 116 on the side close to the base 200, the micro-pump mounting groove 116 is arranged on the groove bottom of the second flow channel groove 114 and is communicated with the second flow channel groove 114, and the micro-pump 310 is arranged in the micro-pump mounting groove 116 to realize the pumping of the liquid. In addition, the flow meter 320 is further provided with a flow meter wire 322, and the micro-pump is further provided with a micro-pump wire 311. Correspondingly, the bottom plate 110 is further provided with a first mounting groove 1151 communicated with the flow detection groove 115 for mounting the flow meter wire 322; the bottom plate 110 is further provided with a second mounting groove 1161 communicated with the micro-pump mounting groove 116 for mounting the micro-pump wire 311; the above structures are all conducive to improving the integration of the micro-fluidic device. It is worth noting that the upper cover 120 is further provided with a relief notch 124 to avoid the installation of the micro-pump wire 311 and the flow meter wire 322.

[0045] Preferably, the micro-pump 310 is arranged at the end of the flow meter 320 away from the fusion cavity. By arranging the flow meter 320 close to the fusion cavity, the accuracy of the volume of the liquid flowing into the fusion cavity is improved, and the detection accuracy is further improved.

[0046] In the embodiment, the micro-flow main body further comprises a sealing plate 420, the bottom plate 110 is provided with a reaction groove 118 at one end away from the upper cover 120, and the sealing plate 420 is connected with the bottom plate 110 to seal the reaction groove 118 to form a reaction cavity. Preferably, the micro-flow main body is further provided with a chip mounting groove 117, the chip mounting groove 117 is arranged corresponding to the reaction cavity, and the temperature control chip 340 is arranged in the chip mounting groove 117. By arranging the chip mounting groove 117 corresponding to the reaction cavity, the distance between the temperature control chip 340 and the reaction cavity is reduced, and the temperature control effect of the temperature control chip on the liquid in the reaction cavity is improved.

[0047] Exemplarily, the bottom plate 110, the upper cover 120, the base 200, the lower cover 410 and the sealing plate 420 are all made of transparent plastic or glass, and specifically can be COC. In addition, the bottom plate 110, the base 200, the lower cover 410 and the sealing plate 420 are connected by bonding or bonding, wherein the bonding can be bonded by glue or double-sided adhesive tape, so as to ensure the sealing between the flow channels, and the specific limitation is not limited.

[0048] The above is only a preferred embodiment of the present application, and for those skilled in the art, according to the idea of the present application, the specific implementation and application range can be changed, and the content of the specification should not be understood as a limitation of the present application.

Claims

1. A microfluidic device, characterized in that, include: The microfluidic body is provided with a first liquid flow channel and a second liquid flow channel. The first liquid flow channel includes a first injection port (121), a mixing communication port (210), and a first equalizing port (122) connected in sequence. The second liquid flow channel includes a second injection port (123), a fusion chamber, a reaction chamber, and a second equalizing port (119) connected in sequence. A control unit is disposed in the microfluidic body. The control unit includes a microfluidic pump (310), a flow meter (320), a droplet generator, and a temperature control chip (340) connected by communication. The microfluidic pump (310) and the flow meter (320) are both disposed between the second injection port (123) and the fusion chamber, and are used to pump a preset amount of the second solution into the fusion chamber. The droplet generator is disposed at the bottom of the reaction chamber and is connected to the mixing communication port (210), and is used to disperse the first solution to mix with the second solution in the fusion chamber to form a mixed solution. The temperature control chip (340) is disposed in the reaction chamber and is used to provide a preset temperature for the mixed solution in the reaction chamber.

2. The microfluidic device according to claim 1, characterized in that, The microfluidic body includes a base, the fusion chamber and the reaction chamber are both disposed on the base, the base is also provided with a first injection channel (111) communicating with the first injection port (121) and a first equalization channel (112) communicating with the first equalization port (122); the microfluidic body is also provided with a base (200), the base (200) is disposed on the side of the base near the bottom of the fusion chamber, the mixing connection port (210) is disposed on the base (200), the base (200) is also provided with a first injection connection port (220) communicating with the first injection channel (111) and a first equalization connection port (230) communicating with the first equalization channel (112), the first injection connection port (220) and the first equalization connection port (230) are both connected to the mixing connection port (210).

3. The microfluidic device according to claim 2, characterized in that, The base includes a base plate (110), on which a first flow channel groove (113) and a second flow channel groove (114) are provided on the side away from the base (200). The reaction chamber and the second equalizing port (119) are both provided on the base plate (110). One end of the first flow channel groove (113) is connected to the reaction chamber, and the other end is provided with a fusion groove (1131). The second flow channel groove (114) is connected to the fusion groove (1131). The base also includes a top cover (120), which is placed on the side of the base plate (110) away from the base (200). The first injection port (121) and the first equalizing port (122) are both provided on the top cover (120). The top cover (120) is provided with a second injection port (123), which is connected to the second flow channel groove (114).

4. The microfluidic device according to claim 3, characterized in that, The base (200) is provided with a connecting groove (240), which is connected to the second flow channel groove (114) and the fusion cavity.

5. The microfluidic device according to claim 3, characterized in that, A flow detection groove (115) is provided on the side of the base plate (110) near the base (200). The flow detection groove (115) is connected to the second flow channel groove (114). The detection end (321) of the flow meter (320) is located in the flow detection groove (115). And / or, a micro pump mounting groove (116) is provided on the side of the base plate (110) near the base (200). The micro pump mounting groove (116) is located at the bottom of the second flow channel groove (114) and is connected to the second flow channel groove (114). The micro pump (310) is located in the micro pump mounting groove (116).

6. The microfluidic device according to claim 3, characterized in that, The base plate (110), the top cover (120), and the base (200) are all made of transparent plastic or glass; the base plate (110), the top cover (120), and the base (200) are connected by adhesive or bonding.

7. The microfluidic device according to claim 2, characterized in that, The microfluidic body also includes a lower cover (410). A through groove (250) is provided on the side of the base (200) away from the seat body. The through groove (250) is connected to the first injection port (220), the first equalizing port (230), and the mixing port (210). The lower cover (410) is placed on the side of the base (200) away from the seat body.

8. The microfluidic device according to claim 7, characterized in that, The droplet generator includes a droplet generating plate (331) and a droplet generating cable (332) that is communicatively connected to the droplet generating plate (331). The droplet generating plate (331) is disposed at the bottom of the fusion cavity and communicates with the mixing port (210). The droplet generating cable (332) is U-shaped and is snapped onto the opposite side of the lower cover (410) and the base (200).

9. The microfluidic device according to any one of claims 1-8, characterized in that, The microfluidic body is also provided with a chip mounting slot (117), which is provided in the reaction chamber, and the temperature control chip (340) is provided in the chip mounting slot (117).

10. The microfluidic device according to any one of claims 1-8, characterized in that, The micro-flow pump (310) is located at the end of the flow meter (320) away from the fusion chamber.

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