Multistage enzymolysis device

Abstract

The utility model relates to the technical field of enzymolysis equipment, in particular to a multi-stage enzymolysis device, at least N groups of enzymolysis components and enzyme deactivation components are arranged in the multi-stage enzymolysis device, the feeding ends of the enzyme deactivation components are respectively connected with the discharging ends of the enzymolysis components, and the discharging ends of the enzyme deactivation components are respectively connected with the feeding ends of the enzymolysis components; each enzymolysis assembly is used for respectively carrying out each stage of enzymolysis reaction, the enzyme deactivation assembly is used for deactivating enzymes so as to prevent the enzymes of each stage of enzymolysis reaction from being mutually mixed, and N is greater than or equal to 2; thus, a first-stage enzymolysis reaction is carried out in one enzymolysis assembly, then an enzymolysis product is sent to the enzyme deactivation assembly, and after enzyme mixed in the enzymolysis product is inactivated, the enzymolysis product is sent to the other enzymolysis assembly to be subjected to a second-stage enzymolysis reaction; the steps from the third-stage enzymolysis reaction to the Nth-stage enzymolysis reaction are the same, so that enzymes of the enzymolysis reactions at all stages are separated, mutual inhibition among different enzymes is prevented, and the production efficiency of a finished product is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to enzyme hydrolysis equipment technical field especially relates to a multistage enzymatic hydrolysis device. BACKGROUND

[0002] The main components of milk (powder) include water, protein, fat, lactose, minerals, vitamins and other components. Protein includes casein, whey protein, lactalbumin and lactoglobulin. Milk peptide is a kind of macromolecular protein in milk degraded into small molecular peptide by scientific biological degradation technology. Small molecular peptide can be easily absorbed, avoid the distress of lactose intolerance population, enhance the human body's disease resistance, improve sleep quality and prevent osteoporosis. Milk peptide can provide basic nutrients for humans, animals, plants and microorganisms, and is widely used in food, nutritional supplements and laboratory research fields. The whole production process of milk peptide is made by steps such as defatting, precipitation, modification, enzymatic hydrolysis, inactivation, filtration and drying.

[0003] Among them, enzymatic hydrolysis is a process of using enzymes to hydrolyze, polymerize and transform substrates. Enzymatic hydrolysis equipment is used to realize this process. The existing enzymatic hydrolysis equipment can only carry out primary enzymatic hydrolysis reaction. According to the main components of milk, the production steps of milk peptide need to carry out multi-stage enzymatic hydrolysis reaction.

[0004] To solve the above technical problems, the technical scheme disclosed by the Chinese invention patent with the patent application number CN201410548925. X is an enzymatic hydrolysis equipment, characterized in that the enzymatic hydrolysis device comprises a tank body, a jacket provided with an inlet and an outlet wrapped around the outer periphery of the tank body, a stirring shaft and a partition plate provided in the tank body, the partition plate separates the tank body into multiple enzymatic hydrolysis spaces along the height direction of the tank body, and the partition plate has a through hole capable of connecting the adjacent two enzymatic hydrolysis spaces separated by the partition plate. The length direction of the stirring shaft in each enzymatic hydrolysis space separated by the partition plate is provided with a stirrer. The enzymatic hydrolysis device further comprises a cellulose-containing raw material inlet, an enzyme inlet and a material outlet communicated with the tank body.

[0005] However, the above-mentioned prior art has the following technical problems: the overall enzymatic hydrolysis reaction of the above-mentioned enzymatic hydrolysis equipment occurs in the same tank body. The enzymes of the primary reaction will enter the enzymatic hydrolysis space of the secondary enzymatic hydrolysis reaction with the primary enzymatic hydrolysis product. Different enzymes will inhibit each other, resulting in the decrease of the reaction speed and product yield of the secondary enzymatic hydrolysis reaction. Similarly, the reaction speed and product yield of the tertiary enzymatic hydrolysis reaction will also decrease, resulting in the decrease of the production efficiency of the finished product. UTILITY MODEL CONTENT

[0006] Therefore, it is necessary to provide a multi-stage enzymatic hydrolysis device, which can separate enzymes of each enzymatic hydrolysis reaction, prevent mutual inhibition between different enzymes, and thus improve production efficiency of finished products.

[0007] The utility model provides a kind of multi-stage enzymatic hydrolysis device, including at least N groups of enzymatic hydrolysis component and enzyme destroying component, the material inlet end of enzyme destroying component is connected with the material outlet end of each enzymatic hydrolysis component respectively, and the material outlet end is connected with the material inlet end of each enzymatic hydrolysis component respectively;Each enzymatic hydrolysis component is used to carry out each enzymatic hydrolysis reaction respectively, and the enzyme destroying component is used to make enzyme lose activity, to prevent enzyme of each enzymatic hydrolysis reaction from mixing each other, wherein N≥2.

[0008] Preferably, each enzymatic hydrolysis component includes enzymatic hydrolysis reaction tank, enzyme material input part, discharge switching part and discharge pipe, the enzyme material input part is installed at the top end of the enzymatic hydrolysis reaction tank, the discharge switching part is provided with an input end and two switchable output ends, the input end of the discharge switching part is connected with the output end of the enzymatic hydrolysis reaction tank, and the two output ends are connected with the discharge pipe and the enzyme destroying component respectively;The enzyme material input part is used to send the enzyme required for enzymatic hydrolysis reaction into the enzymatic hydrolysis reaction tank, the enzymatic hydrolysis reaction tank is used for enzymatic hydrolysis reaction, the discharge switching part is used to send the product of enzymatic hydrolysis reaction to the discharge pipe or the enzyme destroying component by switching operation, and the discharge pipe is used to output the final finished product reaction product.

[0009] Preferably, the outside of each enzymatic hydrolysis reaction tank is provided with a sampling part for sampling and checking from the enzymatic hydrolysis reaction tank.

[0010] Preferably, the enzyme destroying component includes enzyme destroying input pipe, enzyme destroying output pipe, filter part and heating enzyme destroying part, one end of the enzyme destroying input pipe is connected with each discharge switching part, the other end is connected with the input end of the filter part, one end of the enzyme destroying output pipe is connected with the output end of the filter part, the other end is connected with the top end of each enzymatic hydrolysis reaction tank, and the heating enzyme destroying part is installed on the enzyme destroying input pipe;The enzyme destroying input pipe is used to send the enzymatic hydrolysis product of each enzymatic hydrolysis reaction tank into the filter part, the heating enzyme destroying part is used to make the enzyme in the enzyme destroying input pipe lose activity by heating, the filter part is used to filter out the enzyme losing activity from the enzymatic hydrolysis product, and the enzyme destroying output pipe is used to send the enzymatic hydrolysis product after enzyme destroying to each enzymatic hydrolysis reaction tank.

[0011] Preferably, the multi-stage enzymatic hydrolysis device further includes a substrate input assembly, the substrate input assembly includes an input pipe and a dosage detection part, the discharge end of the input pipe is installed at the top end of any enzymatic hydrolysis reaction tank, and the dosage detection part is installed on the input pipe;The input pipe is used to input the substrate required for enzymatic hydrolysis reaction into the enzymatic hydrolysis reaction tank, and the dosage detection part is used to obtain the volume data of the substrate input into the enzymatic hydrolysis reaction tank from the input pipe.

[0012] Preferably, the multi-stage enzymatic hydrolysis device further comprises a positive pressure assembly, the positive pressure assembly comprises a positive pressure fan and a positive pressure air hole opened at the top end of each enzymatic hydrolysis reaction tank, the air outlet end of the positive pressure fan is connected with the top end of each enzymatic hydrolysis reaction tank respectively; the positive pressure fan is used for blowing sterile gas into each enzymatic hydrolysis reaction tank, and the positive pressure air hole is used for exhausting air outward.

[0013] Preferably, the multi-stage enzymatic hydrolysis device further comprises at least N groups of stirring assemblies with the same structure, each stirring assembly is installed on each enzymatic hydrolysis assembly correspondingly, and each stirring assembly comprises a stirring motor, a stirring rod, a plurality of upper stirring blades with the same structure and a plurality of lower stirring blades with the same structure; the fixed end of the stirring motor is installed at the top end of the enzymatic hydrolysis reaction tank, one end of the stirring rod is drivingly connected with the driving end of the stirring motor, the other end is rotationally connected with the tank bottom of the enzymatic hydrolysis reaction tank, each upper stirring blade is fixedly installed on the stirring rod and located at the middle part of the enzymatic hydrolysis reaction tank, and each lower stirring blade is fixedly installed on the stirring rod and located at the bottom of the enzymatic hydrolysis reaction tank; the bottom end of each lower stirring blade is fan-shaped to tightly adhere to the tank wall of the bottom of the enzymatic hydrolysis reaction tank.

[0014] Preferably, the multi-stage enzymatic hydrolysis device further comprises N groups of temperature control assemblies with the same structure, each temperature control assembly is installed on each enzymatic hydrolysis assembly correspondingly, and each temperature control assembly comprises a temperature control pipe, a heating element, a cooling element and a heat preservation shell; the temperature control pipe is wound around the tank body of the enzymatic hydrolysis reaction tank, the top end of the temperature control pipe is connected with the output end of the heating element and the input end of the cooling element respectively, the bottom end of the temperature control pipe is connected with the input end of the heating element and the output end of the cooling element respectively, and the heat preservation shell is arranged outside the tank body of the enzymatic hydrolysis reaction tank and covers the temperature control pipe; the temperature control pipe is used for heating or cooling the enzymatic hydrolysis reaction tank, the heating element is used for providing heat source for the temperature control pipe, the cooling element is used for providing cold source for the temperature control pipe, and the heat preservation shell is used for isolating the temperature control pipe from the outside.

[0015] Preferably, each enzymatic hydrolysis reaction tank is provided with a temperature detection element and a pressure detection element, the temperature detection element is used for collecting temperature data in each enzymatic hydrolysis reaction tank, and the pressure detection element is used for collecting air pressure data in each enzymatic hydrolysis reaction tank.

[0016] Preferably, the multi-stage enzymatic hydrolysis device further comprises a control terminal, the control terminal is communicatively connected with the dosage detection element, the pressure detection element and the temperature detection element, so as to upload the data acquired by the dosage detection element, the pressure detection element and the temperature detection element to the control terminal; and the control terminal is electrically connected with the enzyme material feeding element, the discharge switching element, the positive pressure fan, the stirring motor, the heating element and the cooling element respectively.

[0017] The multi-stage enzymolysis device comprises at least N groups of enzymolysis assemblies and enzyme-killing assemblies, the input ends of the enzyme-killing assemblies are connected with the output ends of the enzymolysis assemblies respectively, and the output ends are connected with the input ends of the enzymolysis assemblies respectively; the enzymolysis assemblies are used for respectively performing the enzymolysis reactions of different stages, and the enzyme-killing assemblies are used for killing the enzymes to prevent the enzymes of the enzymolysis reactions of different stages from mixing with each other, wherein N≥2; in this way, the first-stage enzymolysis reaction is performed in one enzymolysis assembly, the enzymolysis product is sent to the enzyme-killing assembly, the enzymes mixed in the enzymolysis product are killed, and then the enzymolysis product is sent to another enzymolysis assembly to perform the second-stage enzymolysis reaction; the steps of the third-stage enzymolysis reaction to the Nth-stage enzymolysis reaction are the same as above, so that the enzymes of the enzymolysis reactions of different stages are separated, the mutual inhibition between different enzymes is prevented, and the production efficiency of the finished product is improved. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 is a perspective view of the multi-stage enzymolysis device of the present application.

[0019] Figure 2 is a perspective view of one enzymolysis assembly in the multi-stage enzymolysis device of the present application.

[0020] Figure 3 is a front view of the enzyme-killing assembly of the present application.

[0021] Figure 4 is a top view of the multi-stage enzymolysis device of the present application.

[0022] Figure 5 is a sectional view of one enzymolysis assembly in the multi-stage enzymolysis device of the present application.

[0023] In the drawings: multi-stage enzymolysis device 10, enzymolysis assembly 20, enzymolysis reaction tank 21, enzyme material feeding part 22, discharge switching part 23, discharge pipe 24, sampling part 25, enzyme-killing assembly 30, enzyme-killing input pipe 31, enzyme-killing output pipe 32, filtering part 33, heating enzyme-killing part 34, substrate feeding assembly 40, feeding pipe 41, feeding amount detecting part 42, positive pressure assembly 50, positive pressure fan 51, positive pressure air hole 52, stirring assembly 60, stirring motor 61, stirring rod 62, upper stirring blade 63, lower stirring blade 64, temperature control assembly 70, temperature control pipe 71, heating part 72, cooling part 73, heat preservation shell 74. DETAILED DESCRIPTION

[0024] The technical scheme and technical effects of the embodiments of the present application are further described in detail in combination with the drawings of the present application.

[0025] Please refer to Figure 1The utility model provides a kind of multistage enzymatic hydrolysis device 10, including at least N groups of enzymatic hydrolysis component 20 and enzyme inactivation component 30, and the inlet end of enzyme inactivation component 30 is connected with the discharge end of each enzymatic hydrolysis component 20 respectively, and the discharge end is connected with the inlet end of each enzymatic hydrolysis component 20 respectively;Each enzymatic hydrolysis component 20 is used to carry out each level enzymatic hydrolysis reaction respectively, and enzyme inactivation component 30 is used to make enzyme lose activity, to prevent enzyme mixing of each level enzymatic hydrolysis reaction;In this way, first level enzymatic hydrolysis reaction is carried out in one enzymatic hydrolysis component 20, and then enzymatic hydrolysis product is sent to enzyme inactivation component 30, and after the enzyme mixed in enzymatic hydrolysis product is inactivated, second level enzymatic hydrolysis reaction is carried out in another enzymatic hydrolysis component 20;The steps of third level enzymatic hydrolysis reaction to N level enzymatic hydrolysis reaction are as above, so that the enzyme of each level enzymatic hydrolysis reaction is separated, to prevent mutual inhibition between different enzymes, so as to improve the production efficiency of finished product.

[0026] Please refer to Figure 2 Further, each enzymatic hydrolysis component 20 includes enzymatic hydrolysis reaction tank 21, enzyme material input part 22, discharge switching part 23 and discharge pipe 24, enzyme material input part 22 is installed at the top end of enzymatic hydrolysis reaction tank 21, discharge switching part 23 is provided with one input end and two switchable output ends, the input end of discharge switching part 23 is connected with the output end of enzymatic hydrolysis reaction tank 21, and the two output ends are connected with discharge pipe 24 and enzyme inactivation component 30 respectively;Enzyme material input part 22 is used to send enzyme required for enzymatic hydrolysis reaction into enzymatic hydrolysis reaction tank 21, enzymatic hydrolysis reaction tank 21 is used to carry out enzymatic hydrolysis reaction, and discharge switching part 23 is used to send the product of enzymatic hydrolysis reaction to discharge pipe 24 or enzyme inactivation component 30 by switching operation, and discharge pipe 24 is used to output the final finished product reaction product, specifically, when still needing next level enzymatic hydrolysis reaction, the product of enzymatic hydrolysis reaction is sent to enzyme inactivation component 30;When next level enzymatic hydrolysis reaction is not needed, the product of enzymatic hydrolysis reaction is sent out from discharge pipe 24.

[0027] In the embodiment, the enzyme material input part 22 realizes the quantification of enzyme material input amount by the spiral pushing structure arranged in the pipe, and the spiral pushing feeding mode can disperse the clumps in the enzyme material and prevent the pipe from being blocked.

[0028] In the embodiment, the discharge switching part 23 selects an electric three-way valve or an electric four-way valve according to the requirement.

[0029] In the embodiment, the outside of each enzymatic hydrolysis reaction tank 21 is provided with a sampling part 25, so as to take sample from the enzymatic hydrolysis reaction tank 21 for inspection, thereby facilitating the staff to check whether the product of each level enzymatic hydrolysis reaction meets the requirement, specifically, the sampling part 25 is a pipeline with a valve, for example, a faucet.

[0030] Please refer to Figure 3Further, the enzyme-killing assembly 30 comprises an enzyme-killing input pipe 31, an enzyme-killing output pipe 32, a filter 33 and a heating enzyme-killing part 34. One end of the enzyme-killing input pipe 31 is connected with each of the discharge switching parts 23, and the other end is connected with the input end of the filter 33. One end of the enzyme-killing output pipe 31 is connected with the output end of the filter 33, and the other end is connected with the top end of each of the enzyme reaction tanks 21. The heating enzyme-killing part 34 is installed on the enzyme-killing input pipe 31. The enzyme-killing input pipe 31 is used to send the enzyme reaction product of each of the enzyme reaction tanks 21 into the filter 33. The heating enzyme-killing part 34 is used to make the enzyme in the enzyme-killing input pipe 31 lose activity by heating. The filter 33 is used to filter out the enzyme losing activity from the enzyme reaction product, thereby improving the purity of the enzyme reaction product of each stage. The enzyme-killing output pipe 32 is used to send the enzyme reaction product after enzyme-killing to each of the enzyme reaction tanks 21 to participate in the next stage of enzyme reaction.

[0031] Please refer to Figure 2 Further, the multi-stage enzyme hydrolysis device 10 further comprises a substrate input assembly 40. The substrate input assembly 40 comprises an input pipe 41 and a dosage detection part 42. The discharge end of the input pipe 41 is installed on the top end of any of the enzyme reaction tanks 21. The dosage detection part 42 is installed on the input pipe 41. The input pipe 41 is used to input the substrate into the enzyme reaction tank 21. The dosage detection part 42 is used to obtain the volume data of the substrate input into the enzyme reaction tank from the input pipe.

[0032] Please refer to Figure 4 Further, the multi-stage enzyme hydrolysis device 10 further comprises a positive pressure assembly 50. The positive pressure assembly 50 comprises a positive pressure fan 51 and a positive pressure air hole 52 opened on the top end of each of the enzyme reaction tanks 21. The air outlet end of the positive pressure fan 51 is connected with the top end of each of the enzyme reaction tanks 21. The positive pressure fan 51 is used to blow sterile gas into each of the enzyme reaction tanks 21. The positive pressure air hole 52 is used to exhaust air outward, thereby adjusting the air pressure in each of the enzyme reaction tanks 21, so that the air pressure in each of the enzyme reaction tanks 21 meets the requirement of the enzyme hydrolysis reaction to be carried out.

[0033] Please refer to Figure 5Further, the multi-stage enzymatic hydrolysis device 10 further comprises at least N sets of stirring assemblies 60 which are identical in structure, each of the stirring assemblies 60 is installed on each of the enzymatic hydrolysis assemblies, each of the stirring assemblies 60 comprises a stirring motor 61, a stirring rod 62, a plurality of upper stirring blades 63 which are identical in structure, and a plurality of lower stirring blades 64 which are identical in structure, the fixed end of the stirring motor 61 is installed at the top end of the enzymatic hydrolysis reaction tank 21, one end of the stirring rod 62 is drivingly connected with the driving end of the stirring motor 61, the other end is rotatably connected with the tank bottom of the enzymatic hydrolysis reaction tank 21, each of the upper stirring blades 63 is fixedly installed on the stirring rod 62 and located at the middle part of the enzymatic hydrolysis reaction tank 21, so as to stir the substrate and the enzyme material and accelerate the enzymatic hydrolysis reaction, each of the lower stirring blades 64 is fixedly installed on the stirring rod 62 and located at the bottom of the enzymatic hydrolysis reaction tank 21, the bottom end of each of the lower stirring blades 64 is in the shape of a fan, so as to tightly adhere to the tank wall of the bottom of the enzymatic hydrolysis reaction tank 21, thereby stirring the tank bottom of the enzymatic hydrolysis reaction tank 21 when rotating and preventing the substrate and the enzyme material from sticking to the tank bottom.

[0034] Please refer to Figure 5 Further, the multi-stage enzymatic hydrolysis device 10 further comprises N sets of temperature control assemblies 70 which are identical in structure, each of the temperature control assemblies 70 is installed on each of the enzymatic hydrolysis assemblies 20, each of the temperature control assemblies 70 comprises a temperature control pipe 71, a heating element 72, a cooling element 73, and a heat preservation shell 74, the temperature control pipe 71 is wound around the tank body of the enzymatic hydrolysis reaction tank 21, the top end of the temperature control pipe 71 is respectively connected with the output end of the heating element 72 and the input end of the cooling element 73, the bottom end of the temperature control pipe 71 is respectively connected with the input end of the heating element 72 and the output end of the cooling element 73, the heat preservation shell 74 is arranged outside the tank body of the enzymatic hydrolysis reaction tank 21 and covers the temperature control pipe 71; the temperature control pipe 71 is used for heating or cooling the enzymatic hydrolysis reaction tank 21, the heating element 72 is used for providing heat source for the temperature control pipe 71, the cooling element 73 is used for providing cold source for the temperature control pipe 71, and the heat preservation shell 74 is used for isolating the temperature control pipe 71 from the outside, so as to control the temperature in each of the enzymatic hydrolysis reaction tanks 21 to meet the requirements of the enzymatic hydrolysis reaction to be performed.

[0035] Further, each of the enzymatic hydrolysis reaction tanks 21 is provided with a temperature detection element and a pressure detection element, the temperature detection element is used for collecting the temperature data in each of the enzymatic hydrolysis reaction tanks, and the pressure detection element is used for collecting the air pressure data in each of the enzymatic hydrolysis reaction tanks.

[0036] Further, the multi-stage enzymatic hydrolysis device further comprises a control terminal, the control terminal is communicatively connected with the dosage detection element, the pressure detection element, and the temperature detection element, so as to upload the data acquired by the dosage detection element, the pressure detection element, and the temperature detection element to the control terminal; the control terminal is electrically connected with the enzyme material feeding element 22, the discharge switching element 23, the positive pressure fan 51, the stirring motor 61, the heating element 72, and the cooling element 73, so as to control the start and stop of the enzyme material feeding element 22, the discharge switching element 23, the positive pressure fan 51, the stirring motor 61, the heating element 72, and the cooling element 73.

[0037] In one embodiment, the control terminal comprises a display for displaying data acquired by the dosage detection device, each pressure detection device and each temperature detection device, and an input device, such as a keyboard, for inputting data. An operator can input data from the input device according to the data displayed on the display to control the enzymatic reaction in each enzymatic reaction tank 21. Specifically, the operator can control the amount of enzyme material fed into each enzymatic reaction tank 21 by controlling each enzyme material feeding device 22, switch the output direction of the enzymatic reaction product in each enzymatic reaction tank 21 by controlling the output direction of each discharge switching device 23, adjust the air pressure in each enzymatic reaction tank 21 by controlling the air volume of the positive pressure fan 51, adjust the stirring speed in each enzymatic reaction tank 21 by controlling the rotating speed of each stirring motor 61, and adjust the temperature in each enzymatic reaction tank 21 by controlling the output of each heating device 72 and each cooling device 73.

[0038] In one embodiment, the control terminal controls each enzyme material feeding device 22, each discharge switching device 23, the positive pressure fan 51, each stirring motor 61, each heating device 72 and each cooling device 73 according to preset parameters. Specifically, the control terminal adjusts the amount of enzyme material fed into each enzymatic reaction tank 21 according to the volume data of the substrate uploaded by the dosage detection device 42, controls the discharge switching device 23 to output the enzymatic reaction product to the enzyme inactivation assembly 30 for the next enzymatic reaction according to the number of enzymatic reactions required for the finished product, controls the positive pressure fan 51 to adjust the air pressure in each enzymatic reaction tank 21 according to the air pressure data uploaded by each pressure detection device, controls the rotating speed of each stirring motor 61 according to the requirements of the enzymatic reaction, and controls each heating device 72 and each cooling device 73 to adjust the temperature in each enzymatic reaction tank 21 according to the temperature data uploaded by each temperature detection device.

[0039] In this embodiment, the control terminal is integrated on the positive pressure fan 51.

[0040] Example 1: Multi-stage enzymatic reaction by increasing the number of enzymatic assemblies 20

[0041] (1) The number of enzymatic assemblies 20 is set according to the number of enzymatic reactions required for product processing. These enzymatic assemblies 20 are sequentially the first enzymatic assembly, the second enzymatic assembly, …, the Nth enzymatic assembly.

[0042] (2) The substrate is fed into the enzymatic reaction tank 21 of the first enzymatic assembly through the substrate feeding device, and the enzyme material is fed into the enzymatic reaction tank 21 of the first enzymatic assembly according to the amount of substrate fed.

[0043] (3) After the enzymatic reaction in the first enzymatic assembly is completed, the enzymatic reaction product is sent to the enzyme inactivation assembly 30, the enzyme material is inactivated by the heat inactivation device, and the inactivated enzyme material is separated from the enzymatic reaction product by the filter 33.

[0044] (4) The treated enzymatic product is sent to the enzymatic reaction tank 21 of the second enzymatic assembly, and the second enzyme inputting element 22 inputs enzyme according to the amount of substrate;

[0045] (5) After the enzymatic reaction in the second enzymatic assembly, the enzymatic product is sent to the enzyme inactivation assembly 30, the enzyme is inactivated by the heat inactivation element, and the inactivated enzyme is separated from the enzymatic product by the filter element 33;

[0046] (6) The above steps are repeated until the product after N-stage enzymatic reaction is obtained.

[0047] Example 2, multi-stage enzymatic reaction by increasing the number of enzyme inputting elements 22

[0048] In this embodiment, only two enzymatic assemblies 20 are needed, which are respectively named as the first enzymatic assembly and the second enzymatic assembly;

[0049] (1) The enzyme inputting elements 22 are arranged on the first enzymatic assembly and the second enzymatic assembly according to the required number of enzymatic reaction stages, and are sequentially named as the first enzyme inputting element 22, the second enzyme inputting element 22, …, the Nth enzyme inputting element 22 according to the enzyme inputting order;

[0050] (2) The substrate is inputted into the enzymatic reaction tank 21 of the first enzymatic assembly by the substrate inputting element, and the first enzyme inputting element 22 inputs enzyme into the enzymatic reaction tank 21 of the first enzymatic assembly according to the amount of substrate;

[0051] (3) After the enzymatic reaction in the first enzymatic assembly, the enzymatic product is sent to the enzyme inactivation assembly 30, the enzyme is inactivated by the heat inactivation element, and the inactivated enzyme is separated from the enzymatic product by the filter element 33;

[0052] (4) The treated enzymatic product is sent to the enzymatic reaction tank 21 of the second enzymatic assembly, and the second enzyme inputting element 22 inputs enzyme into the enzymatic reaction tank 21 of the second enzymatic assembly according to the amount of substrate;

[0053] (5) After the enzymatic reaction in the second enzymatic assembly, the enzymatic product is sent to the enzyme inactivation assembly 30, the enzyme is inactivated by the heat inactivation element, and the inactivated enzyme is separated from the enzymatic product by the filter element 33;

[0054] (6) The treated enzymatic product is sent to the enzymatic reaction tank 21 of the first enzymatic assembly, and the third enzyme inputting element 22 inputs enzyme into the enzymatic reaction tank 21 of the second enzymatic assembly according to the amount of substrate;

[0055] (7) The above process is repeated until the product after N-stage enzymatic reaction is obtained.

[0056] The above merely describes the preferred embodiments of the present application, and is not intended to limit the present application, and any modification, equivalent replacement, and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A multistage enzymatic hydrolysis apparatus, characterized by, The multistage enzymatic hydrolysis device comprises at least N groups of enzymatic hydrolysis assemblies and enzyme inactivation assemblies, the input ends of the enzyme inactivation assemblies are connected with the output ends of the enzymatic hydrolysis assemblies respectively, and the output ends are connected with the input ends of the enzymatic hydrolysis assemblies respectively; each enzymatic hydrolysis assembly is used for performing a corresponding enzymatic hydrolysis reaction, and the enzyme inactivation assemblies are used for inactivating the enzymes to prevent the enzymes of the enzymatic hydrolysis reactions from mixing with each other, wherein N≥2.

2. The multi-stage enzymatic digestion device of claim 1, wherein, Each enzymatic hydrolysis assembly comprises an enzymatic hydrolysis tank, an enzyme input element, a discharge switching element and a discharge pipe, the enzyme input element is installed at the top end of the enzymatic hydrolysis tank, the discharge switching element is provided with an input end and two switchable output ends, the input end of the discharge switching element is connected with the output end of the enzymatic hydrolysis tank, and the two output ends are connected with the discharge pipe and the enzyme inactivation assembly respectively; the enzyme input element is used for feeding the enzymes required by the enzymatic hydrolysis reaction into the enzymatic hydrolysis tank, the enzymatic hydrolysis tank is used for performing the enzymatic hydrolysis reaction, and the discharge switching element is used for feeding the product of the enzymatic hydrolysis reaction to the discharge pipe or the enzyme inactivation assembly through switching operation, and the discharge pipe is used for outputting the final product of the reaction.

3. The multi-stage enzymatic digestion device of claim 2, wherein, The outer side of each enzymatic hydrolysis tank is provided with a sampling element for sampling and checking the enzymatic hydrolysis tank.

4. The multi-stage enzymatic digestion device of claim 2, wherein, The enzyme inactivation assembly comprises an enzyme inactivation input pipe, an enzyme inactivation output pipe, a filter element and a heating enzyme inactivation element, one end of the enzyme inactivation input pipe is connected with each discharge switching element, the other end is connected with the input end of the filter element, one end of the enzyme inactivation output pipe is connected with the output end of the filter element, the other end is connected with the top end of each enzymatic hydrolysis tank, and the heating enzyme inactivation element is installed on the enzyme inactivation input pipe; the enzyme inactivation input pipe is used for feeding the enzymatic hydrolysis product of each enzymatic hydrolysis tank into the filter element, the heating enzyme inactivation element is used for inactivating the enzymes in the enzyme inactivation input pipe through heating, the filter element is used for filtering the inactivated enzymes from the enzymatic hydrolysis product, and the enzyme inactivation output pipe is used for feeding the enzymatic hydrolysis product after enzyme inactivation to each enzymatic hydrolysis tank.

5. The multistage enzymatic digestion device of claim 2, wherein, The multistage enzymatic hydrolysis device further comprises a substrate input assembly, the substrate input assembly comprises an input pipe and a dosage detection element, the output end of the input pipe is installed at the top end of any enzymatic hydrolysis tank, and the dosage detection element is installed on the input pipe; the input pipe is used for feeding the substrate required by the enzymatic hydrolysis reaction into the enzymatic hydrolysis tank, and the dosage detection element is used for obtaining the volume data of the substrate fed into the enzymatic hydrolysis tank from the input pipe.

6. The multi-stage enzymatic digestion device of claim 5, wherein, The multistage enzymatic hydrolysis device further comprises a positive pressure assembly, the positive pressure assembly comprises a positive pressure fan and a positive pressure air hole opened at the top end of each enzymatic hydrolysis tank, and the air outlet end of the positive pressure fan is connected with the top end of each enzymatic hydrolysis tank respectively; the positive pressure fan is used for blowing sterile gas into each enzymatic hydrolysis tank, and the positive pressure air hole is used for exhausting air.

7. The multi-stage enzymatic digestion device of claim 6, wherein, The multistage enzymolysis device further comprises at least N groups of stirring assemblies which are identical in structure, each of which is installed on a corresponding enzymolysis assembly, and each stirring assembly comprises a stirring motor, a stirring rod, a plurality of upper stirring blades which are identical in structure, and a plurality of lower stirring blades which are identical in structure, the fixed end of the stirring motor is installed at the top end of the enzymolysis reaction tank, one end of the stirring rod is drivingly connected to the driving end of the stirring motor, the other end is rotationally connected to the tank bottom of the enzymolysis reaction tank, each upper stirring blade is fixedly installed on the stirring rod and located in the middle part of the enzymolysis reaction tank, and each lower stirring blade is fixedly installed on the stirring rod and located at the bottom of the enzymolysis reaction tank; the bottom end of each lower stirring blade is fan-shaped to tightly adhere to the tank wall of the bottom of the enzymolysis reaction tank.

8. The multi-stage enzymatic digestion device of claim 7, wherein, The multistage enzymolysis device further comprises N groups of temperature control assemblies which are identical in structure, each of which is installed on a corresponding enzymolysis assembly, and each temperature control assembly comprises a temperature control pipe, a heating element, a cooling element, and a heat preservation shell, the temperature control pipe is wound around the tank body of the enzymolysis reaction tank, the top end of the temperature control pipe is respectively connected to the output end of the heating element and the input end of the cooling element, the bottom end of the temperature control pipe is respectively connected to the input end of the heating element and the output end of the cooling element, and the heat preservation shell is arranged outside the tank body of the enzymolysis reaction tank and covers the temperature control pipe; the temperature control pipe is used for heating or cooling the enzymolysis reaction tank, the heating element is used for providing a heat source for the temperature control pipe, the cooling element is used for providing a cold source for the temperature control pipe, and the heat preservation shell is used for isolating the temperature control pipe from the outside world.

9. The multistage enzymatic digestion device of claim 8, wherein, Each enzymolysis reaction tank is provided with a temperature detection element and a pressure detection element, the temperature detection element is used for collecting temperature data in each enzymolysis reaction tank, and the pressure detection element is used for collecting air pressure data in each enzymolysis reaction tank.

10. The multistage enzymatic digestion device of claim 9, wherein, The multistage enzymolysis device further comprises a control terminal, the control terminal is in communication connection with the dosage detection element, the pressure detection element, and the temperature detection element, so as to upload the data acquired by the dosage detection element, the pressure detection element, and the temperature detection element to the control terminal, and the control terminal is in electrical connection with the enzyme material feeding element, the discharge switching element, the positive pressure fan, the stirring motor, the heating element, and the cooling element.

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