High-pressure dewaxing thermal repair device for immunohistochemistry

Abstract

The utility model relates to a high-pressure dewaxing thermal repair device for automatic immunohistochemistry. And the lifting mechanism and the temperature control module are integrated, so that automatic operation of the thermal repair process is realized. The design of the stepping motor and the supporting mechanism enables the lifting of the top cover to be automatic, so that the test sample carrier placing area is automatically exposed or closed, and the complexity and potential errors of manual operation are effectively reduced. Through the design and layout optimization of the heating element, the heating and the automatic lifting mechanism of the top cover are accurately controlled, the total time required by thermal repair is shortened, and the device has important significance for accelerating the experimental process and improving the working efficiency of a laboratory. The temperature control module is electrically connected with the temperature probe, so that the temperature of the heating element can be monitored and adjusted in real time, experimental conditions are ensured to accord with preset parameters, an automatic control system reduces variables caused by improper manual operation, and the stability and accuracy of the experimental process are enhanced.

Description

TECHNICAL FIELD

[0001] The utility model belongs to high pressure hot repair technical field, more specifically relates to a kind of high pressure dewaxing hot repair device for automation immunohistochemistry. BACKGROUND

[0002] Immunohistochemistry experiment is important research method in biology, medicine and other fields. Currently, the most commonly used fixative for tissue fixation is formaldehyde. During the process of tissue fixation, formaldehyde will cross-link with protein, forming a kind of methylene bridge cross-linking structure, so that the antigenic determinant in the tissue is shielded. Heating can destroy the protein cross-linking caused by formaldehyde fixation, so that the shielded antigenic determinant is exposed again, so that it can be combined with the corresponding antibody. High pressure hot repair step plays a key role in improving the exposure of antigen, enhancing the sensitivity of immunohistochemical reaction and the quality of final staining. However, the existing high pressure hot repair is generally operated by artificial, and the operation of high pressure hot repair equipment is often complex, so that the automatic in and out of the slide cannot be realized, which greatly affects the experimental efficiency. Therefore, it has important practical application value to design a device capable of automatically performing high pressure immunohistochemical hot repair and automatically transferring the slide. SUMMARY

[0003] In view of the above technical problems, the utility model provides a kind of high pressure dewaxing hot repair device for immunohistochemistry, to realize the automation of high pressure hot repair process and the automatic transfer of slide, improve the effect of hot repair, shorten the hot repair time and accurately control the temperature and time of hot repair, so as to improve the accuracy, stability and experimental efficiency of repair.

[0004] To achieve the above object, the utility model provides a kind of high pressure dewaxing hot repair device for immunohistochemistry, comprising:

[0005] Reactor main body, the reactor main body has reactor wall, top cover and bottom cover, the reactor wall and bottom cover are enclosed to form accommodating cavity, and the accommodating cavity is used to accommodate test sample carrier;

[0006] Heating element is arranged in the reactor wall and / or accommodating cavity;

[0007] Temperature probe is arranged in the top cover;

[0008] Test sample carrier placement area;

[0009] The lifting mechanism comprises a stepping motor, a screw rod, a bracket and a support rod, the stepping motor is arranged on the bracket, connected with a top cover located directly below the stepping motor through the screw rod, the top cover is connected with the test sample carrier placement area through a connecting rod, the maximum distance between the top cover and the test sample carrier placement area is less than or equal to the longitudinal depth of the accommodating cavity, and the support rod supports the bracket through the edge of the top cover and is used for lifting the top cover along the support rod.

[0010] The control unit comprises a temperature control module and a driving module, the temperature control module is electrically connected with the temperature probe and used for controlling the heating element based on the temperature reading of the temperature probe, and the driving module is electrically connected with the lifting mechanism and used for driving the lifting mechanism to move upward or downward.

[0011] Further, the high-pressure dewaxing thermal repair device for immunohistochemistry further comprises an automatic transfer module connected to the control unit, and the automatic transfer module is electrically connected with a component comprising a three-dimensional motion platform, a turnover motor, a mechanical arm, a first electromagnetic valve, a suction cup, a vacuum interface and a vacuum pump.

[0012] The mechanical arm is connected to the three-dimensional motion platform through a fixed shaft, the mechanical arm rotates around the fixed shaft as the axis, the turnover motor is electrically connected with the mechanical arm and controlled by the control unit, the vacuum interface is arranged in the suction cup and connected with the pipeline of the vacuum pump, the suction cup is arranged on the mechanical arm, and the vacuum pump is controlled by the first electromagnetic valve to realize the suction and release of the test sample carrier.

[0013] Such a design realizes the automatic transfer of the test sample carrier between the processing stations by integrating the three-dimensional motion platform and the mechanical arm, not only reduces the need for manual operation, but also greatly improves the processing speed and accuracy. The mechanical arm cooperates with the three-dimensional motion platform to accurately control the position of the test sample carrier in space, ensures the accurate placement of the test sample carrier during thermal repair and dewaxing, and reduces operation errors. The automatic transfer module improves the repeatability and accuracy of the experiment by accurately controlling the movement and positioning of the test sample carrier, which is particularly important for scientific research and clinical diagnosis. The application of the automatic transfer module makes the entire experimental process without manual intervention, realizes fully automated operation, and significantly improves the overall efficiency of the experiment and the operation efficiency of the laboratory. Moreover, since manual operation is reduced, the test sample carrier reduces contact with the outside world during the transfer process, effectively reducing the risk of cross contamination. At the same time, the automatic transfer module is designed simply, which is convenient for daily maintenance and inspection, reduces the maintenance cost and complexity of the equipment. These improvements comprehensively enhance the functionality and practicality of the device, making the device more efficient and reliable in the field of biochemical analysis such as immunohistochemistry.

[0014] Further, the immunohistochemistry high-pressure dewaxing and heat repairing device further comprises a gas pressure detection module arranged in the pipeline passage between the suction cup and the vacuum pump, for detecting whether the suction cup adsorbs the test sample carrier. In this way, by monitoring the gas pressure state of the suction cup in real time, the gas pressure detection module can accurately determine whether the slide is successfully adsorbed, ensuring the reliability of the test sample carrier transfer and processing process, and avoiding experimental failure or repeated work due to the failure of the suction cup to correctly adsorb the test sample carrier. The gas pressure detection module can automatically detect the functional state of the suction cup system, such as whether the suction cup is leaking or the pipeline is blocked, reducing the complexity and frequency of equipment maintenance, while preventing possible operation errors in advance. Ensuring accurate adsorption of the test sample carrier each time helps to maintain the consistency and repeatability of the experiment, which is crucial for accurate interpretation and subsequent analysis of experimental data. By reducing the loss of test sample carriers and repeated experiments due to operation errors, the gas pressure detection module helps to save experimental materials and valuable time, improve overall experimental efficiency, and further improve the automation level of the entire device, making the device operation more intelligent and automatic, and reducing the dependence on operator skills. The gas pressure detection module not only improves the safety and reliability of the experiment, but also significantly improves the performance of the entire device and the effectiveness of the experiment by improving the accuracy and automation of the operation.

[0015] Some improved immunohistochemistry high-pressure dewaxing and heat repairing devices further comprise a water pump and a second electromagnetic valve connected to the water pump, controlled by a control unit to realize water injection into the accommodation cavity or water discharge from the accommodation cavity. In these improved embodiments, the combination of the water pump and the second electromagnetic valve enables the control unit to accurately control water injection into the accommodation cavity or water discharge from the accommodation cavity, which helps to maintain the specific humidity and temperature conditions required during the experiment, thereby improving the accuracy and repeatability of the experimental results. Automated water flow management reduces the need for manual operation, improves the automation level of the experiment, optimizes the workflow, and reduces the possibility of human error during operation. During the high-pressure dewaxing and heat repairing process, timely water flow control can effectively regulate the temperature in the accommodation cavity, speed up the dewaxing and repairing process, save experimental time, and improve overall efficiency. Precise control of water discharge can effectively prevent cross-contamination, especially in the case of continuous processing of multiple samples, a clean working environment is crucial for maintaining the purity of the samples and the accuracy of the experiment. Precise water flow management ensures that the accommodation cavity can be effectively cleaned after each experiment, ensuring that there is no residual material affecting the next experiment.

[0016] Further, the accommodating cavity is provided with a water outlet, a water inlet and an exhaust port. The water outlet is arranged at the lower part of the accommodating cavity close to the bottom cover. The water inlet is arranged at the middle upper part of the accommodating cavity close to the top cover. The exhaust port is arranged at the upper part of the accommodating cavity close to the top cover. Such arrangement follows the pressure difference between the water inlet and the water outlet, so that the water drainage and water inlet can be achieved most quickly, and the gas can be easily discharged.

[0017] Further, the test sample carrier placement area is provided with a plurality of vertical grooves which are oppositely arranged and matched for vertically inserting or pulling out the test sample carrier. By vertically inserting or pulling out the test sample carrier, the test sample carrier can be accessed more quickly, thereby improving the overall work efficiency, especially in experiments involving a large number of samples. More importantly, when the test sample carrier is a commonly used glass slide, the vertical insertion design can reduce the high-pressure contact area, thereby reducing the high pressure that the glass slide is subjected to during high-pressure repair, and avoiding the risk of glass breakage. The vertical design of the grooves allows the test sample carrier to be stored vertically, achieving uniform dewaxing and repair, and more effectively utilizing the space within the device, so that the device can store more glass slides in a smaller space, which is suitable for experimental environments with limited space. In addition, the grooves provide stable support and protection for the test sample carrier, ensuring the stability of the test sample carrier during high-pressure and high-temperature thermal repair, and avoiding experimental failure caused by displacement of the test sample carrier.

[0018] Further, the lower part of the reactor body is further provided with a base.

[0019] Still further, the periphery of the reactor body is further provided with a protective sleeve. This can enhance the safety of the entire device and avoid accidental injury to the experimenter.

[0020] In some preferred embodiments, a transfer module is further provided for placing the test sample carrier after high-pressure dewaxing and thermal repair. Such arrangement facilitates the subsequent operation of the mechanical arm and suction cup group to suck the repaired test sample carrier into the next processing step.

[0021] In more preferred embodiments, the top cover is matched with the reactor body and opens or seals the reactor with the support rod rising or descending. It can be understood that the matching degree of the top cover plays an important role in the overall aesthetics and sealing performance of the device, and can well play the dewaxing and repair functions.

[0022] Distinguishing from the prior art, the above technical scheme realizes the automatic operation of the thermal repair process by integrating the lifting mechanism and the temperature control module. The design of the stepping motor and the supporting mechanism makes the lifting of the top cover automatic, thereby automatically exposing or closing the test sample carrier placement area, effectively reducing the complexity and potential errors of manual operation. Through the design and layout optimization of the heating element, uniform heating of the test sample in the accommodation cavity is ensured, thereby improving the effect of thermal repair, which is particularly important for immunohistochemical processes that require precise control of heating temperature and time. By precisely controlling the heating and the automatic lifting mechanism of the top cover, the present application shortens the total time required for thermal repair, which is of great significance for speeding up the experimental process and improving the efficiency of laboratory work. The electrical connection of the temperature control module and the temperature probe allows real-time monitoring and adjustment of the temperature of the heating element, ensuring that the experimental conditions meet the preset parameters, and precise control helps to ensure the repeatability and reliability of the experimental results. The automated control system reduces the variables caused by improper human operation, enhancing the stability and accuracy of the experimental process. The above technical scheme effectively solves the problems of traditional manual operation, such as complexity, time consumption, and inaccurate temperature control, providing a more efficient and reliable technical solution for immunohistochemical experiments.

[0023] The above description is only a summary of the technical scheme of the present application. In order for those skilled in the art to more clearly understand the technical scheme of the present application, and to implement the content described in the specification and drawings, and in order for the above and other purposes, features and advantages of the present application to be more easily understood, the following describes the specific embodiments of the present application and the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0024] The accompanying drawings are only used to illustrate the principles, implementation methods, applications, characteristics and effects of the specific embodiments of the present application and other related content, and cannot be considered as limiting the present application.

[0025] In the drawings of the specification:

[0026] Figure 1 The high-pressure dewaxing thermal repair device for immunohistochemistry provided by the embodiment 1 of the present application is shown in the schematic diagram.

[0027] Figure 2 The control unit structure schematic diagram containing the temperature control module and the driving module in the embodiment 1 of the present application is shown.

[0028] Figure 3 The more automated high-pressure dewaxing thermal repair device for immunohistochemistry provided by the embodiment 2 of the present application is shown in the schematic diagram.

[0029] Figure 4The control unit structure schematic view of the automatic transfer module of the utility model embodiment 3 contains temperature control module, drive module, automatic transfer module and air pressure detection module;

[0030] Figure 5 The control unit structure schematic view of the automatic transfer module of the utility model embodiment 3 contains temperature control module, drive module, automatic transfer module and air pressure detection module;

[0031] Figure 6 The high-pressure dewaxing heat repair device containing cavity structure schematic view provided by the utility model embodiment 5 for immunohistochemistry is provided;

[0032] Figure 7 The test sample carrier glass slide placement area structure schematic view of the high-pressure dewaxing heat repair device for immunohistochemistry provided by the utility model embodiment 6 is provided.

[0033] The reference signs involved in the above-mentioned various drawings are explained as follows:

[0034] 1, reactor main body;10, reactor wall;11, top cover;12, bottom cover;111, containing cavity;112, water outlet;113, water inlet;114, exhaust port;

[0035] 20, heating element;

[0036] 30, temperature probe;

[0037] 4, test sample carrier placement area;41, groove;

[0038] 5, lifting mechanism;50, stepper motor;51, lead screw, 52, support;53, support rod;54, connecting rod;

[0039] 6, control unit;61, temperature control module;62, drive module;63, automatic transfer module;64, air pressure detection module;

[0040] 71, three-dimensional motion platform;72, overturning motor;73, mechanical arm;74, first electromagnetic valve;75, suction cup;

[0041] 8, protective sleeve. DETAILED DESCRIPTION

[0042] In order to explain the possible application scenarios, technical principles, specific schemes that can be implemented, purposes and effects that can be achieved of the present application in detail, the following will be described in detail in combination with the specific embodiments listed and the accompanying drawings. The embodiments described in this paper are only used to more clearly explain the technical solutions of the present application, therefore only as an example, and cannot limit the protection scope of the present application.

[0043] The term "embodiment" is mentioned herein means that the specific features, structures, or characteristics described in connection with the embodiment can be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit the independence or association between other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the technical features mentioned in each embodiment can be combined in any way to form a corresponding implementable technical solution.

[0044] Unless otherwise defined, the meanings of the technical terms used herein are the same as those commonly understood by those skilled in the art to which the present application belongs; the use of related terms herein is only for the purpose of describing specific embodiments, and is not intended to limit the present application.

[0045] In the description of the present application, the phrase "and / or" is a description of the logical relationship between the objects, which means that there can be three relationships, for example, A and / or B, which means that there are three cases: A exists, B exists, and A and B exist at the same time. In addition, the character " / " herein generally represents that the associated objects before and after are a "or" logical relationship.

[0046] In the present application, terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual quantity, primary and secondary, or order relationship between the entities or operations.

[0047] In the present application, without more limitation, the "includes", "contains", "has" or other similar expressions used in the sentence are intended to cover non-exclusive inclusion, and these expressions do not exclude the presence of other elements in the process, method or product including the described elements, so that the process, method or product including a series of elements can not only include those limited elements, but also include other elements not explicitly listed, or also include the elements inherent to such process, method or product.

[0048] As the same as the understanding in the "Guidelines for Examination", in the present application, the expressions "greater than", "less than", "exceed" and the like are understood as not including the number; the expressions "above", "below", "within" and the like are understood as including the number. In addition, in the description of the embodiments of the present application, the meaning of "multiple" is more than two (including two), and similar expressions related to "multiple" are also understood in this way, for example, "multiple groups", "multiple times" and the like, unless otherwise explicitly limited.

[0049] In the description of the embodiments of the present application, the spatially relative terms, such as "central", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "vertical", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or position relationship shown in the specific embodiment or the accompanying drawings, and are only for the convenience of describing the specific embodiments of the present application or for the reader to understand, and do not indicate or imply that the indicated device or element must have a particular position, a particular orientation, or be constructed or operated in a particular orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.

[0050] Unless otherwise expressly specified or limited, the terms "mount", "connect", "connection", "fixed", "set", and the like used in the description of the embodiments of the present application should be interpreted broadly. For example, the "connection" can be a fixed connection, or a detachable connection, or an integral setting; it can be a mechanical connection, or an electrical connection, or a communication connection; it can be a direct connection, or an indirect connection through an intermediate medium; it can be a communication or interaction relationship between two elements. For those skilled in the art to which the present application belongs, the specific meaning of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0051] The traditional immunohistochemical high-pressure steam method dewaxing heat repair operation steps include:

[0052] Slice preparation: Use anti-dewaxing glass slides to ensure that the slices are complete and undamaged.

[0053] Dewaxing and hydration: through a series of solvent treatment, the tissue slices are released from the wax and recover water, so that the antigen repair solution can fully penetrate into the tissue.

[0054] Antigen repair solution preparation: select appropriate antigen repair buffer, such as TivEDTA pH9.0 or sodium citrate pH6.0, and add it to the pressure cooker.

[0055] High-pressure repair: place the pot on the heating plate and turn it to the maximum power, but do not cover the lid tightly. After the antigen repair solution is boiled, carefully take the slides out of the tap water and put them into the pressure cooker, making sure that the slides are completely immersed in the repair solution. Add the pressure valve, and when the pressure cooker reaches the maximum pressure, maintain it for a period of time (usually 2-3 minutes).

[0056] Cooling: turn off the heating plate, remove the pressure cooker, open the valve for cold water cooling. After the pressure is reduced, open the lid and continue to cool with cold water for a period of time (such as 10 minutes) to slowly restore the antigen repair solution to room temperature.

[0057] Subsequent treatment: after the repair liquid is restored to room temperature, PBS washing is performed, and then subsequent staining and slicing operations are performed according to the selected immunohistochemical staining method.

[0058] The above operation has large errors, large differences between links, low work efficiency of manual operation, and the like, and in addition, health hazards caused by contact with toxic reagents are easily caused.

[0059] Based on the above, the utility model provides an immunohistochemical high-pressure dewaxing heat repair device to solve the above technical problems. Embodiment

[0060] Please refer to Figure 1 , Figure 2The utility model provides a kind of high-pressure dewaxing thermal repair device for immunohistochemistry, including reactor main body 1, heating element 20, temperature probe 30, TR glass sheet placement area 4, lifting mechanism 5 and control unit 6.Reactor main body 1 needs to satisfy the dewaxing repair condition of immunohistochemistry in biomedical field, for example, heating uniformity, durable safety, easy cleaning, compatible with commonly used immunohistochemistry reagent and solution etc., it can be for example stainless steel, aluminum alloy, ceramic etc. Material, shape is not particularly limited, including but not limited to cylindrical, square, rectangle, but preferred is stainless steel cylindrical pot.Reactor main body 1 has reactor wall 10, top cover 11 and bottom cover 12, reactor wall 10 and bottom cover 12 are enclosed to form accommodating cavity 111, and accommodating cavity 111 is used to accommodate test sample.In the dewaxing repair process, test sample is the slide group to be dewaxed and repaired.Heating element 20 is for example electric heating wire, electric heating tube, electric heating plate, electromagnetic coil, electric heating film, graphite heating element etc., is separately arranged in reactor wall or accommodating cavity, or is simultaneously arranged in reactor wall and accommodating cavity, but preferably is separately arranged in reactor wall, to avoid generating occupation and unnecessary test risk in accommodating cavity.Temperature probe 30 is arranged on top cover.Test sample carrier glass sheet placement area 4 in the embodiment is the slide rack of basket or box shape made of material capable of being placed horizontally or vertically and capable of bearing high temperature above 60 DEG C, containing slide limiting structure.Test sample carrier glass sheet placement area 4 is the slide rack of basket or box shape made of material capable of being placed horizontally or vertically and capable of bearing high temperature above 60 DEG C, containing slide limiting structure.Lifting mechanism 5 includes stepping motor 50, screw rod 51, support 52 and support rod 53 and some fixed screws, nuts and other accessories, stepping motor 50 is fixedly arranged on support 52, is connected with top cover 11 located directly below stepping motor 50 by screw rod 51, top cover 11 is connected with glass sheet placement area 4 by connecting rod 54, and the maximum distance between top cover 11 and glass sheet placement area 4 is less than or equal to the depth of accommodating cavity 111, so that when top cover descends along with screw rod, glass sheet placement area also gradually descends into accommodating cavity, and it can be ensured that top cover can be completely closed with the upper part of reactor main body.Support rod 53 supports support 52 through the edge of top cover 11, and is used for lifting movement of top cover 11 along support rod 53.Control unit 6 includes temperature control module 61 and drive module 62, temperature control module 61 is electrically connected with temperature probe 30, for controlling heating element 20 based on the temperature reading of temperature probe 30, and drive module 62 is electrically connected with lifting mechanism 5, for driving lifting mechanism 5 to move upward or downward.

[0061] These embodiments realize the automation operation of thermal repair process, automatically expose or close glass sheet placement area, effectively reduce the complexity and potential error of manual operation. The total time required for thermal repair is shortened, which is of great significance for accelerating experimental process and improving laboratory efficiency. Embodiments

[0062] Please refer to Figure 3 , Figure 4The embodiment proposes a more automated high-pressure dewaxing and heat repairing device for immunohistochemistry. Based on the device in Embodiment 1, an automatic transfer module 63 connected to the control unit 6 is further provided. The automatic transfer module 63 is electrically connected with an automatic transfer assembly including a three-dimensional motion platform 71, a turnover motor 72, a mechanical arm 73, a first electromagnetic valve 74, a suction cup 75, a vacuum interface (not shown), and a vacuum pump (not shown). The mechanical arm is connected to the three-dimensional motion platform through a fixed shaft. The mechanical arm performs a turnover motion with the fixed shaft as the axis. The turnover motor is electrically connected with the mechanical arm and controlled by the control unit. The vacuum interface is arranged in the suction cup and connected with the vacuum pump pipeline. The suction cup is arranged on the mechanical arm. The vacuum pump realizes the suction and release of the slide by the control of the first electromagnetic valve.

[0063] In these embodiments, the slides are automatically transferred between processing stations, reducing the need for manual operation and greatly improving processing speed and accuracy. The mechanical arm combined with the three-dimensional motion platform can accurately control the position of the slide in space, ensuring accurate placement of the slide during heat repair, dewaxing, and other processes, and reducing operational errors. The automatic slide transfer module improves the repeatability and accuracy of the experiment by precisely controlling the movement and positioning of the slide, allowing the entire experimental process to be performed without human intervention, achieving fully automated operation, and significantly improving the overall efficiency of the experiment and the operation efficiency of the laboratory. Moreover, as manual operation is reduced, the slide reduces contact with the outside world during transfer, effectively reducing the risk of cross-contamination. Embodiment

[0064] Please refer to Figure 5 Based on Embodiment 2, the high-pressure dewaxing and heat repairing device for immunohistochemistry further includes a gas pressure detection module 64 arranged in the pipeline passage between the suction cup and the vacuum pump, for detecting and determining whether the suction cup is adsorbed with the slide. Such a setting can ensure the reliability of the slide transfer and processing process by determining whether the slide is successfully adsorbed, avoiding experimental failure or repeated work due to the failure of the suction cup to correctly adsorb the slide. Embodiment

[0065] Based on Embodiment 3, the high-pressure dewaxing and heat repairing device for immunohistochemistry further includes a water pump (not shown) and a second electromagnetic valve (not shown). The water pump is connected with the second electromagnetic valve and controlled by the control unit to realize water injection into the accommodation cavity or water discharge from the accommodation cavity. Embodiment

[0066] Please refer to Figure 6 Based on Embodiment 4, the accommodation cavity of the high-pressure dewaxing and heat repairing device for immunohistochemistry is provided with a water outlet 112, a water inlet 113, and an exhaust port 114. The water outlet 112 is arranged at the lower part of the accommodation cavity close to the bottom cover. The water inlet 113 is arranged at the middle upper part of the accommodation cavity close to the top cover. The exhaust port 114 is arranged at the upper part of the accommodation cavity close to the top cover. Embodiment

[0067] Referring to Figure 7 The sample carrier slide placement area 4 of the high-pressure dewaxing and heat repair device for immunohistochemistry is provided with a plurality of groove bodies 41 arranged opposite to each other and matched with each other for vertically inserting or pulling out the slide. By vertically inserting and pulling out the slide, the slide access operation can be performed more quickly, thereby improving the overall work efficiency, especially in experiments involving a large number of samples. More importantly, the vertical insertion design can reduce the high-pressure contact area, thereby reducing the pressure intensity to which the slide is subjected during the high-pressure repair process, and avoiding the risk of slide breakage. The groove body design allows the slide to be stored vertically, achieving uniform and consistent dewaxing and repair, and can more effectively utilize the space within the device, so that the device can store more slides in a smaller space, which is suitable for experimental environments with limited space. In addition, the groove body also provides stable support and protection for the slide, ensuring the stability of the position of the slide during the high-pressure and high-temperature heat repair process, and avoiding experimental failure caused by slide displacement. Embodiment

[0068] Referring again to Figure 3 The lower part of the reactor main body of the high-pressure dewaxing and heat repair device for immunohistochemistry is further provided with a base, and the periphery is further provided with a protective sleeve 8. In addition, a transfer module is further provided for placing the slides after high-pressure dewaxing and heat repair.

[0069] The operation process of the high-pressure dewaxing and heat repair device for immunohistochemistry provided in the present application is as follows:

[0070] (1) Device start-up and preheating:

[0071] The user starts the device through the touch screen interface and selects the preset dewaxing and heat repair program. The control unit starts the heating element and preheats the reactor to the target temperature, for example, sets the temperature between 65°C and 75°C.

[0072] (2) Sample loading:

[0073] Open the top cover of the device through the corresponding button of the touch screen interface, place the slide containing the tissue section in the slide placement area, start the stepping motor to lower, and let the top cover, temperature probe, connecting rod and slide placement area together descend to the containing cavity of the reactor main body, and then close the top cover.

[0074] (3) Automatic heat repair and dewaxing:

[0075] The temperature probe monitors the temperature in the containing cavity in real time and adjusts the heating element to maintain the set temperature.

[0076] (4) Cooling and sample removal:

[0077] After the heat treatment is completed, the heating is stopped, and the control unit instructs the lifting mechanism to gradually lift the cover by the stepper motor, and the user can take out the slide.

[0078] (5) Equipment cleaning and shutdown:

[0079] After the user removes all test samples, simple cleaning and maintenance are performed, and then the equipment is turned off through the control unit.

[0080] In a more preferred embodiment, the computer system control can be used to realize the automatic processing of the high-pressure dewaxing and heat repair device for immunohistochemistry in the present application, and the operation process is as follows:

[0081] (1) Automatic slide loading:

[0082] The user stacks the slides to be processed in the automatic loading area. The vacuum pump of the automatic slide transfer module is started, the suction cup adsorbs the slide at the top, and the mechanical arm moves it to the slide placement area.

[0083] (2) Heat repair and dewaxing:

[0084] The control unit adjusts the height of the lifting mechanism and the temperature of the heating element according to the preset program to achieve efficient heat repair. The entire process is carried out in a closed reactor to prevent heat loss and speed up the processing speed.

[0085] (3) Automatic slide transfer:

[0086] After the heat repair is completed, the automatic transfer module is started again, and the mechanical arm moves the processed slide to the cooling area or directly to the transfer module.

[0087] (4) Continuous operation and monitoring:

[0088] The control unit records the processing parameters and time, and the user can monitor the processing status of each slide through the interface. The system supports continuous operation, and multiple sample processing can be completed without manual intervention.

[0089] Finally, it should be noted that although the above embodiments have been described in the specification and drawings of the present application, this does not limit the patent protection scope of the present application. Any equivalent structure or equivalent process substitution or modification based on the essential concept of the present application, using the content described in the specification and drawings, and directly or indirectly implementing the technical solutions of the above embodiments in other related technical fields, etc. are all included in the patent protection scope of the present application.

Claims

1. A high-pressure dewaxing thermal repair device for immunohistochemistry, characterized by, The reactor body has a reactor wall, a top cover and a bottom cover, the reactor wall and the bottom cover enclose a containing cavity for accommodating a test sample carrier; A heating element is arranged in the reactor wall and / or the containing cavity; A temperature probe is arranged on the top cover; A test sample carrier placement area; A lifting mechanism, which comprises a stepper motor, a screw rod, a bracket and a support rod, the stepper motor is arranged on the bracket and connected to the top cover below the stepper motor through the screw rod, the top cover is connected to the test sample carrier placement area through a connecting rod, the maximum distance between the top cover and the test sample carrier placement area is less than or equal to the depth of the containing cavity, and the support rod supports the bracket through the edge of the top cover and is used for the upward or downward movement of the top cover along the support rod; A control unit, which comprises a temperature control module and a driving module, the temperature control module is electrically connected to the temperature probe and used for controlling the heating element based on the temperature reading of the temperature probe, and the driving module is electrically connected to the lifting mechanism and used for driving the lifting mechanism to move upward or downward. An automatic transfer module connected to the control unit, which is electrically connected to a component comprising a three-dimensional motion platform, a turnover motor, a mechanical arm, a first electromagnetic valve, a suction cup, a vacuum interface and a vacuum pump; 2. The high pressure dewaxing thermal repair device for immunohistochemistry according to claim 1, characterized in that, The mechanical arm is connected to the three-dimensional motion platform through a fixed shaft, the mechanical arm rotates around the fixed shaft, the turnover motor is electrically connected to the mechanical arm and controlled by the control unit, the vacuum interface is arranged in the suction cup and connected to the pipeline of the vacuum pump, the suction cup is arranged on the mechanical arm, and the vacuum pump is controlled by the first electromagnetic valve to realize the suction and release of the test sample carrier. A gas pressure detection module is arranged in the pipeline between the suction cup and the vacuum pump to detect whether the suction cup adsorbs the test sample carrier.

3. The high pressure dewaxing thermal repair device for immunohistochemistry according to claim 2, characterized in that, A water pump and a second electromagnetic valve are connected to the control unit to realize water injection into the containing cavity or water discharge from the containing cavity.

4. The immunohistochemistry high-pressure dewaxing thermal repair device according to claim 1, characterized in that, The containing cavity is provided with a water outlet, a water inlet and an exhaust port, the water outlet is arranged at the lower part of the containing cavity close to the bottom cover, the water inlet is arranged at the middle upper part of the containing cavity close to the top cover, and the exhaust port is arranged at the upper part of the containing cavity close to the top cover.

5. The immunohistochemistry high-pressure dewaxing thermal repair device according to claim 4, characterized in that, The test sample carrier placement area is provided with a plurality of vertical grooves arranged opposite to each other and matched with each other for vertically inserting or pulling out the test sample carrier.

6. The immunohistochemistry high-pressure dewaxing thermal repair device according to claim 1, characterized in that, The lower part of the reactor body is further provided with a base.

7. The immunohistochemistry high-pressure dewaxing thermal repair device according to claim 1, characterized in that, The periphery of the reactor body is further provided with a protective sleeve.

8. The immunohistochemistry high-pressure dewaxing thermal repair device according to claim 1, characterized in that, A transfer module is further arranged for placing the test sample carrier after high-pressure dewaxing and hot repair.

9. The immunohistochemistry high-pressure dewaxing thermal repair device according to claim 1, characterized in that, The top cover is matched with the reactor body and opens or seals the reactor with the upward or downward movement of the support rod.

10. The immunohistochemistry high-pressure dewaxing thermal repair device according to claim 1, characterized in that, ​

Images