Temperature control and uniform mixing integrated device for marking high-capacity latex

Abstract

The utility model relates to a temperature control and uniform mixing integrated device for marking high-capacity latex, which is applied to the technical field of biological detection. The temperature control and uniform mixing integrated device comprises a mixing barrel, a stirrer, a water pump and a heater, wherein the stirrer is arranged above the mixing barrel; the mixing barrel comprises an outer-layer barrel and an inner-layer barrel arranged in the outer-layer barrel, a heating liquid outlet is formed in the upper part of the outer-layer barrel, and a heating liquid inlet is formed in the lower part of the outer-layer barrel; the stirrer comprises a rotating motor, a stirrer shell arranged on the outer side of the rotating motor and a supporting ruler fixed to the shell at the bottom of the stirrer, an output shaft of the rotating motor is fixed to an adjusting gear, and the adjusting gear is fixed to a paddle; by adopting the large-capacity mixing barrel, the latex marking device can perform one-time detection on a large number of samples, and in addition, the device is additionally provided with the heater and the stirrer, so that the device can perform heating and heat preservation on the interior and perform stirring at any time.

Description

Technical Field

[0001] This utility model relates to an integrated temperature control and mixing device, and in particular to an integrated temperature control and mixing device for large-capacity latex labeling applied in the field of biological detection technology. Background Technology

[0002] Latex labeling is a commonly used technique in biological detection and diagnosis. Its main principle is to use latex microspheres to combine with biomolecules such as labeled proteins, antibodies or nucleic acids, thereby forming stable complexes that facilitate subsequent detection and analysis.

[0003] Patent publication number CN111239418A discloses a kit for determining ANA based on latex-enhanced immunoturbidimetric assay, comprising a reaction buffer R1 and a latex reagent R2. The reaction buffer R1 includes a buffer solution, inorganic salts, a turbidifying agent, a preservative, a stabilizer, and a surfactant. The latex reagent R2 comprises at least five latex reagent components formed by coupling ANA antigens with latex microspheres. Each latex reagent component includes a latex preservation solution and ANA antigen-labeled latex microspheres preserved in the latex preservation solution. The type of ANA antigen used in each latex reagent component is different. Its advantages include: ANA can be detected using a fully automated biochemical analyzer; it is simple to operate, fast in detection, has high precision, good repeatability, and the detection results are not affected by operation. However, it cannot simultaneously detect a large number of biomolecules.

[0004] To address the issue that most detection methods cannot simultaneously detect a large number of biomolecules, this solution proposes an integrated temperature control and mixing device for large-capacity latex labeling. Utility Model Content

[0005] The technical problem that this invention aims to solve in view of the above-mentioned prior art is that most detection methods cannot detect a large number of biomolecules simultaneously.

[0006] To address the aforementioned problems, this utility model provides an integrated temperature control and mixing device for marking large-capacity latex, comprising a mixing tank, a stirrer positioned above the mixing tank, and a water pump and a heater both mounted on top of the stirrer. The mixing tank includes an outer tank and an inner tank located inside the outer tank. The upper part of the outer tank has a heating liquid outlet, and the lower part has a heating liquid inlet. The heating liquid outlet is connected to the water pump inlet via a pipe, the water pump outlet is connected to the heater inlet via a pipe, and the heater outlet is connected to the heating liquid inlet via a pipe. The stirrer includes a rotary motor, a stirrer housing positioned outside the rotary motor, and a support ruler fixed to the bottom housing of the stirrer. The output shaft of the rotary motor is fixed to an adjusting gear, and the adjusting gear is fixed to a paddle.

[0007] The aforementioned integrated temperature control and mixing device for large-volume latex labeling enables the simultaneous testing of a large number of samples.

[0008] As a further improvement of this application, the bottom of the stirrer housing is provided with a hole, and a circular rack that meshes with the adjusting gear is installed on the side wall of the hole.

[0009] As a further improvement of this application, a guide block is provided at the bottom of the end of the support ruler away from the adjusting gear, and a guide groove corresponding to the position of the guide block is opened at the bottom of the agitator shell.

[0010] As a further improvement of this application, the outlet of the heater is fitted with an inlet valve, and the inlet of the heating liquid is fitted with an outlet valve. The outlet of the inlet valve and the inlet of the outlet valve are connected by a transparent pipe.

[0011] As another improvement of this application, the contact surface between the rotary motor and the agitator housing is smoothly provided, and the contact surface between the agitator housing and the support ruler is smoothly provided.

[0012] As a further improvement to this application, both the outer and inner drums are made of stainless steel, and a gap is provided between the outer and inner drums.

[0013] In summary, when this latex marking device is running, the heater heats the liquid in the pipes, and the water pump circulates the liquid. The heated liquid enters the mixing tank from the heating liquid inlet of the outer tank. At this point, the heating liquid heats the mixture in the mixing tank. When the temperature of the mixture in the mixing tank is close to that of the heating liquid, the heating liquid then acts as a heat-preserving agent for the mixture. Simultaneously, the rotary motor of the stirrer also operates. When the rotary motor rotates, both the blades and the adjusting gear rotate. The rotation of the blades agitates the mixture in the mixing tank, allowing the latex microspheres and biomolecules to combine rapidly. Furthermore, the stirring of the stirrer while the heating liquid is heating the mixture helps to ensure uniform heating of the mixture. The rotation of the adjusting gear interacts with the bottom of the stirrer housing, causing the position of the blades to change, thereby increasing the stirring range of the stirrer. Attached Figure Description

[0014] Figure 1 This is a perspective view of the latex marking device according to the first embodiment of this application;

[0015] Figure 2 This is a front cross-sectional view of the latex marking device according to the first embodiment of this application;

[0016] Figure 3 This is an exploded view of the stirrer according to the first and second embodiments of this application;

[0017] Figure 4 This is a perspective view of the stirrer structure according to the first and second embodiments of this application;

[0018] Figure 5 This is a three-dimensional structural diagram of the latex marking device according to the second embodiment of this application.

[0019] Explanation of the labels in the diagram:

[0020] 1. Mixing tank; 11. Outer tank; 12. Inner tank; 13. Heating liquid outlet; 14. Heating liquid inlet; 2. Stirrer; 21. Stirrer housing; 22. Rotary motor; 23. Paddle; 24. Support ruler; 25. Adjusting gear; 3. Water pump; 4. Heater; 51. Inlet valve; 52. Outlet valve. Detailed Implementation

[0021] The two embodiments of this application will be described in detail below with reference to the accompanying drawings.

[0022] First implementation method:

[0023] Please see Figures 1-2An integrated temperature control and mixing device for large-volume latex marking includes a mixing tank 1, a stirrer 2 positioned above the mixing tank 1, and a water pump 3 and a heater 4 both mounted on top of the stirrer 2. The mixing tank 1 includes an outer tank 11 and an inner tank 12 positioned inside the outer tank 11. The upper part of the outer tank 11 has a heating liquid outlet 13, and the lower part of the outer tank 11 has a heating liquid inlet 14. This arrangement ensures that when the mixing tank 1 is heated, water flows in from the heating liquid inlet at the lower part of the outer tank 11. The water flows into the mixing tank 1 through outlet 14 and then out through the heating liquid outlet 13 at the top of the outer tank 11. This effectively retains the water flow within the mixing tank 1, preventing the heating liquid from rapidly passing through the mixing tank 1 under gravity, which could lead to poor heating performance. Furthermore, the incoming water from the lower layer impacts and replaces the original lower layer water, thus improving heating and insulation efficiency. The heating liquid outlet 13 is connected to the inlet of the water pump 3 via a pipe. Preferably, the water pump 3 is of type [missing information - likely a specific type]. The water pump DS5004 is a miniature car electronic water pump powered by an external power source. The outlet of the water pump 3 is connected to the inlet of the heater 4 via a pipe, and the outlet of the heater 4 is connected to the heating liquid inlet 14 via a pipe. The agitator 2 includes a rotary motor 22, an agitator housing 21 located outside the rotary motor 22, and a support ruler 24 fixed to the bottom housing of the agitator 2. Preferably, the rotary motor 22 is a 57BL75S10-230TF9 brushless DC motor, which is powered by an external power source connected to the water pump 3. In addition, since there are holes at the bottom of the agitator housing 21, the purpose of the support ruler 24 is to support the rotary motor 22 and prevent it from falling out of the agitator housing 21. The output shaft of the rotary motor 22 is fixed to the adjusting gear 25, and the adjusting gear 25 is fixed to the blade 23. Through the above connection, the agitator 2 can drive the rotation of the blade 23 and change the stirring position of the blade 23 when only one rotary motor 22 is used.

[0024] In summary, when this latex labeling device is in operation, the biomolecules to be detected and the latex microspheres used for detection are first poured into the mixing tank 1. After the device is powered on, the heater 4 heats the liquid in the pipeline, and the water pump 3 causes the liquid in the pipeline to flow. The heated liquid enters the interior of the mixing tank 1 from the heating liquid inlet 14 of the outer tank 11. At this time, the heating liquid heats the mixture in the mixing tank 1. When the temperature of the mixture in the mixing tank 1 is close to that of the heating liquid, the heating liquid becomes a heat-preserving agent for the mixture. Meanwhile, the rotary motor 22 of the stirrer 2 will also run. When the rotary motor 22 rotates, the blade 23 and the adjusting gear 25 will also rotate. The rotation of the blade 23 will stir the mixture in the mixing tank 1, so that the latex microspheres and biomolecules can combine quickly. When the heating liquid heats the mixture, the stirring of the stirrer 2 will also help to make the mixture heat evenly. The rotation of the adjusting gear 25 will interact with the bottom of the stirrer housing 21, causing the position of the blade 23 to change, thereby increasing the stirring range of the stirrer 2.

[0025] In addition, the bottom of the agitator housing 21 has a hole, and a circular rack that meshes with the adjusting gear 25 is installed on the side wall of the hole. The rotary motor 22 drives the adjusting gear 25 to interact with the circular rack, so that the agitator rotates along the path of the circular rack, thereby increasing the agitation range of the blade 23. A guide block is provided at the bottom of the support ruler 24 away from the adjusting gear 25, and a guide groove is provided at the bottom of the agitator housing 21 corresponding to the guide block. Through the interaction between the guide block and the guide groove, and the support of the support ruler 24 on the rotary motor 22, the adjusting gear 25 can smoothly run in a circle along the circular rack. The contact between the rotary motor 22 and the agitator housing 21 The contact surfaces of the stirrer shell 21 and the support ruler 24 are made smooth. By making all the contact surfaces smooth, the internal device of the stirrer 2 will not have large frictional wear during rotation, thereby increasing the service life of the latex marking device. The outer tank 11 and the inner tank 12 are both made of stainless steel. Since both the inner and outer sides of the outer tank 11 and the inner tank 12 need to be in contact with the solution, stainless steel is required for both the inner tank 12 and the outer tank 11 in order to improve the service life of the mixing tank 1. A gap is provided between the outer tank 11 and the inner tank 12. The purpose of this gap is to facilitate the flow of the heating liquid in the mixing tank 1, which can effectively facilitate the heating and heat preservation of the mixture.

[0026] Second implementation method:

[0027] Please see Figures 3-5Unlike the first embodiment, the outlet of the heater 4 is fitted with an inlet valve 51, which has an inlet that can be controlled to open and close. This inlet valve 51 makes it easier to add heating liquid. The heating liquid inlet 14 is fitted with an outlet valve 52, which has an outlet that can be controlled to open and close. This outlet valve 52 makes it easier to remove heating liquid. The outlet of the inlet valve 51 and the inlet of the outlet valve 52 are connected by a transparent pipe, through which the clear liquid level of the heating liquid can be seen, facilitating the addition and removal of developing solution.

[0028] The latex marking device can be equipped with an inlet valve 51 at the outlet of the heater 4 and an outlet valve 52 at the heating liquid inlet 14. When the heating water in the latex marking device needs to be replaced, the heating liquid can be drawn out by opening the control valve of the outlet valve 52. Compared with not installing the outlet valve 52, the operation after installation is more convenient and can prevent unnecessary leakage of heating liquid when the pipeline is disassembled. Then, close the control valve of the outlet valve 52 and open the control valve of the inlet valve 51. The heating liquid can be added to the latex marking device through the inlet of the inlet valve 51. During the addition process, the water level can be observed through the transparent pipe between the inlet valve 51 and the outlet valve 52 to prevent over-addition of heating liquid. As mentioned above, after installing the outlet valve 52 and the inlet valve 51, the process of adding and replacing heating water in the latex marking device is simpler and more convenient.

[0029] In light of current practical needs, the above-described embodiments adopted in this application are not limited to these. Any changes made within the scope of knowledge possessed by those skilled in the art without departing from the concept of this application still fall within the protection scope of this utility model.

Claims

1. A temperature control and mixing integrated device for large volume latex marking, characterized in that, It includes a mixing barrel (1), a stirrer (2) arranged above the mixing barrel (1), and a water pump (3) and a heater (4) both mounted on the top of the stirrer (2); The mixing barrel (1) comprises an outer barrel (11) and an inner barrel (12) arranged inside the outer barrel (11), the upper part of the outer barrel (11) is provided with a heating liquid outlet (13), the lower part of the outer barrel (11) is provided with a heating liquid inlet (14), the heating liquid outlet (13) is connected with the water inlet of the water pump (3) through a pipeline, the water outlet of the water pump (3) is connected with the water inlet of the heater (4) through a pipeline, and the water outlet of the heater (4) is connected with the heating liquid inlet (14) through a pipeline; the stirrer (2) comprises a rotating motor (22), a stirrer shell (21) arranged outside the rotating motor (22), and a support ruler (24) fixed with the bottom shell of the stirrer (2), the output shaft of the rotating motor (22) is fixed with an adjusting gear (25), and the adjusting gear (25) is fixed with a paddle (23).

2. The temperature control and mixing integrated device for large capacity latex marking according to claim 1, characterized in that, The bottom of the stirrer shell (21) is provided with a hole, and the side wall of the hole is provided with a circular rack engaged with the adjusting gear (25).

3. The temperature control and mixing integrated device for large volume latex marking according to claim 1, characterized in that, The bottom of the support ruler (24) away from the adjusting gear (25) is provided with a guide block, and the bottom of the stirrer shell (21) is provided with a guide groove corresponding to the position of the guide block.

4. The temperature control and mixing integrated device for large volume latex marking according to claim 1, characterized in that, The water outlet of the heater (4) is sleeved with an inlet valve (51), and the heating liquid inlet (14) is sleeved with an outlet valve (52), and the water outlet of the inlet valve (51) and the water inlet of the outlet valve (52) are connected through a transparent pipeline.

5. The temperature control and mixing integrated device for large volume latex marking according to claim 1, characterized in that, The contact surface of the rotating motor (22) and the stirrer shell (21) is smoothly arranged, and the contact surface of the stirrer shell (21) and the support ruler (24) is smoothly arranged.

6. The temperature control and mixing integrated device for large volume latex marking according to claim 1, characterized in that, The outer barrel (11) and the inner barrel (12) are both made of stainless steel material, and a gap is arranged between the outer barrel (11) and the inner barrel (12).

Images