Continuous row centrifugal tube consistent with centrifugal tube rack, cryopreservation box and centrifugal machine in position

Abstract

The utility model provides a row centrifuge tube which is consistent with a centrifuge tube rack, a cryopreservation box and a centrifuge in position, and relates to the field of centrifuge tube preparation, the row centrifuge tube comprises tube bodies and tube covers, a plurality of tube covers are fixedly connected side by side, and the tube covers are connected with the tube bodies in a one-to-one matching manner; each tube cover comprises a tail part, a cover body and a head part, one end of the cover body is connected with the tail part, the tail part is connected to the tube body through a connecting belt, the other end of the cover body is connected with the head part, and two adjacent tube covers are connected through a flexible connecting piece; or the two adjacent tube covers are directly connected. A plurality of centrifugal tubes are placed side by side, so that the problem that the centrifugal tubes need to be placed one by one and replaced is solved, and the efficiency is greatly improved; a plurality of centrifugal tubes are connected, so that the problem that the working efficiency is greatly reduced due to the fact that each centrifugal tube needs to be repeatedly opened and closed in the operation process is solved, and the centrifugal tube covers can be opened and closed in batches through the continuous row design; and by adopting the design of the continuous row centrifugal tubes, different sample sizes and operation habits of different operators are met, the experiment is quickly completed, and the working time is saved.

Description

Technical Field

[0001] This utility model relates to the field of centrifuge tube preparation, specifically to a row of centrifuge tubes positioned in the same location as centrifuge tube racks, cryopreservation boxes, and centrifuges. Background Technology

[0002] In clinical testing, a large number of samples (mostly blood and urine samples) are processed daily, typically ranging from 100 to 300. The preprocessing of these samples requires numerous centrifuge tubes, primarily 1.5 mL and 2.0 mL. A typical procedure involves: 1) Arranging the centrifuge tubes in a rack or cryovial (first arrangement). The sample to be tested is placed in each tube (tube #1). Next, a precipitant or other reagent is added to precipitate proteins or dilute the sample. After adding the reagent, the tubes are capped and thoroughly vortexed to mix (first capping). During vortexing, the caps must be tightly closed to prevent splashing and errors. 2) After precipitation, the centrifuge tubes are arranged one by one... Place the tubes in a centrifuge (second placement of centrifuge tubes), centrifuge at high speed. After centrifugation, remove the centrifuge tubes one by one and place them back into the centrifuge tube rack or cryovial (third placement of centrifuge tubes). Then open the centrifuge tube (first opening of centrifuge tube cap) and transfer the supernatant to a new centrifuge tube (tube number 2; tube number 2 needs to be placed in advance, i.e., fourth placement of centrifuge tubes) for the next step; 3) Next, other reagents need to be added to the new centrifuge tube (tube number 2). Taking liquid-liquid extraction as an example, the liquid-liquid extraction extractant needs to be added. After adding the extractant, 4) Close the centrifuge tube cap again (second time closing the centrifuge tube cap) and vortex thoroughly; 5) Place the centrifuge tube in the centrifuge (fifth time placing the centrifuge tube), and centrifuge at high speed to complete the separation of the two liquids in the liquid-liquid extraction; 6) After centrifugation, remove the centrifuge tube from the centrifuge and place it back in the centrifuge tube rack or cryopreservation box (sixth time placing the centrifuge tube), open the centrifuge tube cap (second time opening the centrifuge tube cap), and transfer one layer of liquid from the liquid-liquid extraction process into a new centrifuge tube (centrifuge tube No. 3, which needs to be placed in advance, i.e., the seventh time placing the centrifuge tube); 7) Obtain the liquid-liquid extraction result. The extraction solvent was dried under nitrogen (i.e., the 8th time the centrifuge tubes were placed). After drying, the centrifuge tubes were placed back in the centrifuge tube rack or cryopreservation box (i.e., the 9th time the centrifuge tubes were placed). The reconstitution solution was added to the centrifuge tubes, and the centrifuge tube caps were closed (the 3rd time the centrifuge tube caps were closed). The tubes were then vortexed thoroughly to dissolve the reconstitution solution. The reconstitution solution was centrifuged at high speed in a centrifuge (i.e., the 10th time the centrifuge tubes were placed). After centrifugation, the centrifuge tubes were placed back in the centrifuge tube rack or cryopreservation box (i.e., the 11th time the centrifuge tubes were placed). The centrifuge tube caps were opened (the 3rd time the centrifuge tube caps were opened). The supernatant was transferred to a 96-well plate and injected into a liquid chromatography-mass spectrometry instrument for analysis.

[0003] The above description, using liquid-liquid extraction as an example, illustrates the process of repeatedly opening and closing centrifuge tube caps during sample pretreatment. Similar processes involving derivatization reactions, solid-phase extraction, and other pretreatment operations involve numerous opening and closing of centrifuge tubes. Furthermore, not only are repeated opening and closing of caps required, but also the replacement of many new centrifuge tubes. These details will not be elaborated further. In summary, actual sample processing involves a significant amount of centrifuge tube replacement and repeated opening and closing of caps. If the sample volume is large and the pretreatment procedures are complex, these repetitive tasks of opening and closing caps and replacing centrifuge tubes can consume a substantial amount of time.

[0004] Based on our practical experience, 1) with skilled operators, closing a centrifuge tube takes approximately 4 seconds, and opening it takes approximately 3 seconds. Therefore, opening and closing a centrifuge tube once takes at least 7 seconds. In liquid-liquid extraction, this requires at least 3 openings and 3 closings, totaling 21 seconds. For 100 samples, this means processing 100 samples takes at least 35 minutes. 2) Each time a centrifuge tube is replaced, it needs to be placed one by one into the centrifuge tube rack, cryopreservation box, or centrifuge. This manual placement of centrifuge tubes also generates a significant workload. Assuming 3 seconds to place one centrifuge tube, even with highly skilled operators, placing 11 centrifuge tubes for 100 samples would take 100 × 11 × 3 = 55 minutes. Therefore, placing and replacing centrifuge tubes, and opening and closing the caps, takes at least 90 minutes, significantly delaying the sample pretreatment process.

[0005] Besides wasting a lot of time on arranging centrifuge tubes and opening and closing the caps, these two tasks are also very tedious, tiring, and boring, often causing a lot of trouble for the operators. Utility Model Content

[0006] In view of the deficiencies in the existing technology, the purpose of this utility model is to provide a row of centrifuge tubes that are aligned with the positions of the centrifuge tube rack, cryopreservation box and centrifuge.

[0007] According to the present invention, a series of centrifuge tubes are arranged in the same position as centrifuge tube racks, cryopreservation boxes and centrifuges, comprising: tube bodies and tube caps, multiple tube caps being fixedly connected side by side, and each tube cap being adapted and connected to the tube body.

[0008] The pipe cap includes a tail, a cap body, and a head. One end of the cap body is connected to the tail, and the tail is connected to the pipe body via a connecting strap. The other end of the cap body is connected to the head. Adjacent pipe caps are connected by a flexible connector; or, adjacent pipe caps are directly connected.

[0009] Preferably, the distance between two adjacent pipe caps is 0-3mm.

[0010] Preferably, when 0 < the distance between two adjacent pipe covers and 1 ≤ mm, two adjacent cover bodies are fixedly connected, and multiple pipe bodies are connected side by side through integrated pipe covers.

[0011] Preferably, when 1 mm < the distance between two adjacent centrifuge tubes ≤ 3 mm, multiple tubes are connected side by side through the cooperation of flexible connectors and tube caps.

[0012] Preferably, the flexible connector is located between two adjacent covers.

[0013] Preferably, the flexible connector is located between two adjacent heads.

[0014] Preferably, the straight-line distance between the flexible connector and the head is 8-10mm.

[0015] Preferably, the straight-line distance between the flexible connector and the head is 0.8-1.2 mm.

[0016] Preferably, the diameter of the cover body in the directly connected pipe cover is greater than the diameter of the cover body in the pipe cover connected by the flexible connector.

[0017] Preferably, the number of tubes is 8, 9, 10 or 12.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) This application arranges multiple centrifuge tubes side by side, which solves the problem of needing to place and replace centrifuge tubes one by one, and greatly improves efficiency;

[0020] (2) This application connects multiple centrifuge tubes, which solves the problem that repeatedly opening and closing the cap of each centrifuge tube during operation will greatly reduce work efficiency. The centrifuge tube caps can be opened and closed in batches through the continuous design.

[0021] (3) This application adopts a design of continuous centrifuge tubes to meet the different sample sizes and different operators' operating habits, which can enable the experimenters to complete the experiment quickly and save about 0.75-3.0 hours of working time per day. Attached Figure Description

[0022] Other features, objects, and advantages of this invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0023] Figure 1 This is a schematic diagram of the overall structure in Embodiment 1;

[0024] Figure 2 This is a schematic diagram of the overall structure in Embodiment 2;

[0025] Figure 3This is a schematic diagram of the overall structure in Embodiment 3.

[0026] The figure shows: 1-pipe cap; 11-tail; 12-cap body; 13-head; 14-flexible connector. Detailed Implementation

[0027] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the present invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0028] Example 1

[0029] According to this utility model, a row of centrifuge tubes is positioned in the same location as the centrifuge tube rack, cryopreservation box, and centrifuge. Figure 1 As shown, it includes: a tube body and a tube cap 1, multiple tube caps 1 are fixedly connected side by side, and each tube cap 1 is fitted and connected to the tube body. The outer diameter of the tube cap 1 is larger than the outer diameter of the tube body, the diameter of the tube body is 13-14 mm, and the volume includes but is not limited to 1.5 mL and 2 mL.

[0030] The pipe cap 1 includes a tail 11, a cap body 12, and a head 13. One end of the cap body 12 is connected to the tail 11, and the tail 11 is connected to the pipe body via a connecting strap. The other end of the cap body 12 is connected to the head 13. The distance between two adjacent pipe caps 1 is 0-3mm, and two adjacent pipe caps 1 are connected by a flexible connector 14.

[0031] like Figure 1 As shown, when 1 mm < the distance between two adjacent centrifuge tubes 1 ≤ 3.0 mm, multiple tubes are connected side-by-side through the cooperation of flexible connector 14 and tube cap 1. Preferably, the flexible connector 14 is located between two adjacent heads 13. The straight-line distance between the flexible connector 14 and the head 13 is 0.8-1.2 mm.

[0032] More specifically, 0 < the distance between two adjacent tube caps 1 ≤ 3.0 mm to accommodate different types of centrifuge tube containers (including centrifuge tube racks, cryopreservation boxes, and centrifuges, etc.). The flexible connector 14 has a width of 1.2-2.0 mm, a length of 4-8.5 mm, and a thickness of 1.2-1.5 mm. This eliminates the need to open and close the caps of centrifuge tubes one by one and replace them individually during the entire experiment, greatly reducing the time spent on such repetitive operations during sample pretreatment.

[0033] The flexible connector 14 has a certain degree of flexibility to facilitate the transfer of centrifuge tubes from centrifuge tube racks and cryovials to centrifuges. Centrifuge tubes are typically arranged in a circular pattern, while those in centrifuge tube racks and cryovials are usually arranged in a straight line. It is important to note that the connecting material between the centrifuge tubes 1 needs to have this flexibility when transitioning from a straight to a circular arrangement. Based on the most common specifications of centrifuge tube racks, cryovials, and centrifuges, the most common connection distances should be: 1) 0.35mm; 2) 1.00mm.

[0034] According to the background information and calculations, "100 samples would require 100 × 11 × 3 = 55 minutes. Therefore, the entire operation, including placing and changing centrifuge tubes and opening and closing the caps, takes at least 90 minutes. This application, however, uses a design with 12×1, 8×1, 10×1, or 9×1 centrifuge tubes in a row to accommodate different sample volumes and different operator habits, allowing researchers to complete experiments quickly and saving approximately 0.75 (processing 50 samples) to 3.0 (processing 200 samples) hours of working time per day."

[0035] Example 2

[0036] This embodiment 2 is based on embodiment 1, mainly changing the position of the flexible connector 14, specifically:

[0037] like Figure 2 As shown, when 1 mm < the distance between two adjacent centrifuge tubes 1 ≤ 3.0 mm, multiple tubes are connected side-by-side through the cooperation of flexible connector 14 and tube cap 1. Preferably, the flexible connector 14 is located between two adjacent caps 12. The straight-line distance between the flexible connector 14 and the head 13 is 8-10 mm. The flexible connector 14 has a width of 1.2-2.0 mm, a length of 0.5-3.5 mm, and a thickness of 1.2-1.5 mm.

[0038] Example 3

[0039] This embodiment 2 is based on embodiment 1, changing the connection relationship between two adjacent pipe caps 1. Specifically:

[0040] like Figure 3 As shown, when 0 < the distance between two adjacent pipe caps 1 ≤ 1mm, the two adjacent pipe caps 1 are directly connected, and in this embodiment, the diameter of the cap 12 is larger than the diameter of the cap 12 in embodiments 1-2. The connection effect is achieved by changing the diameter of the pipe cap; the diameter of the pipe cap 1 is 13.15mm-13.30mm. Preferably, two adjacent caps 12 are fixedly connected, and multiple pipes are connected side by side through the integrally connected pipe caps 1.

[0041] In the description of this application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0042] The specific embodiments of this utility model have been described above. It should be understood that this utility model is not limited to the specific embodiments described above, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the substantive content of this utility model. Unless otherwise specified, the embodiments and features described in this application can be arbitrarily combined with each other.

Claims

1. A connected row of centrifuge tubes in alignment with a centrifuge tube rack, cryo box and centrifuge location, characterized by, The utility model relates to a centrifugal tube, which comprises: a tube body and a tube cover (1), a plurality of the tube cover (1) is connected side by side, and the tube cover (1) is connected with the tube body one by one; the tube cover (1) includes a tail (11), a cover body (12) and a head (13), one end of the cover body (12) is connected with the tail (11), the tail (11) is connected on the tube body through a connecting belt, the other end of the cover body (12) is connected with the head (13), and adjacent two tube covers (1) are connected through a flexible connecting piece (14); or, adjacent two tube covers (1) are directly connected.

2. The aligned stackable centrifuge tubes of claim 1, wherein, The distance between adjacent two tube covers (1) is 0-3mm.

3. The aligned stackable centrifuge tubes of claim 2, wherein, When 0 < the distance between adjacent two tube covers (1) ≤1mm, adjacent two cover bodies (12) are fixedly connected, and a plurality of tube bodies are connected side by side through the integrally connected tube cover (1).

4. The aligned stackable centrifuge tubes of claim 2, wherein, When 1mm < the distance between adjacent two centrifugal tubes ≤3mm, a plurality of tube bodies are connected side by side through the cooperation of the flexible connecting piece (14) and the tube cover (1).

5. The aligned stackable centrifuge tubes of claim 4, wherein, The flexible connecting piece (14) is located between adjacent two cover bodies (12).

6. The aligned stackable centrifuge tubes of claim 4, wherein, The flexible connecting piece (14) is located between adjacent two heads (13).

7. The aligned stackable centrifuge tubes of claim 5, wherein, The straight line distance of the flexible connecting piece (14) from the head (13) is 8-10mm.

8. The aligned stackable centrifuge tubes of claim 6, wherein, The straight line distance of the flexible connecting piece (14) from the head (13) is 0.8-1.2mm.

9. The aligned stackable centrifuge tubes of claim 6, wherein, The diameter of the cover body (12) in the directly connected tube cover (1) is greater than the diameter of the cover body (12) in the tube cover (1) connected by the flexible connecting piece (14).

10. The aligned stackable centrifuge tubes of claim 1, wherein, The number of the tube body is 8, 9, 10 or 12.

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