Micro-fluidic chip

Abstract

The utility model discloses a micro-fluidic chip. The micro-fluidic chip comprises a chip body and a rubber connecting piece, the chip body is internally provided with a detection cavity, the detection cavity is connected with a liquid inlet flow channel and a liquid outlet flow channel, the liquid inlet flow channel is communicated with a liquid inlet, and the liquid outlet flow channel is communicated with a liquid outlet; the rubber connecting pieces are arranged at the liquid inlet and the liquid outlet, the rubber connecting pieces are connected with the inner wall of the liquid inlet / liquid outlet in a sealed mode, and the rubber connecting pieces are provided with liquid through holes communicated with the liquid inlet flow channel / liquid outlet flow channel. According to the micro-fluidic chip, the liquid inlet and the liquid outlet are provided with the rubber connecting pieces, and the rubber connecting pieces can be used for being in sealed butt joint with an injection device / a liquid discharging device, so that the sealing performance when the micro-fluidic chip is in butt joint with the injection device / the liquid discharging device is improved, and the condition of liquid leakage is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological analysis detection technical field, in particular to a kind of microfluidic chip. BACKGROUND

[0002] Microfluidic is a kind of scientific technology with the main feature of accurately manipulating fluid in micro-nano scale space, with the ability to miniaturize the basic functions of biological, chemical and other laboratories such as sample preparation, reaction, separation and detection to a few square centimeter chip, and its basic characteristics and greatest advantage is that various unit technologies are flexibly combined and scale integrated on an overall controllable micro platform. Microfluidic chip has the advantages of light volume, small amount of sample and reagent, fast reaction speed, large-scale parallel processing and disposable use, and has great potential in biological, chemical, medical and other fields. The micron-level structure of microfluidic chip has high requirements for the docking place between microfluidic chip and injection device / liquid discharge device. At present, the docking place of microfluidic chip and injection device / liquid discharge device is not well sealed, which leads to the problem of liquid leakage during injection / liquid discharge. SUMMARY

[0003] Therefore, the utility model provides a kind of microfluidic chip, to solve the problem of microfluidic chip and easily appear liquid leakage when injecting / liquid.

[0004] The scheme provided by the utility model comprises:

[0005] A kind of microfluidic chip, comprising:

[0006] Chip body and rubber connecting piece;

[0007] Detection cavity is equipped in the chip body, the detection cavity is connected with liquid inlet flow channel and liquid outlet flow channel, the liquid inlet flow channel is communicated with liquid inlet, and the liquid outlet flow channel is communicated with liquid outlet;

[0008] The rubber connecting piece is arranged in the liquid inlet and the liquid outlet, the rubber connecting piece is sealedly connected with the inner wall of the liquid inlet / liquid outlet, and the rubber connecting piece is equipped with liquid passage hole communicated with the liquid inlet flow channel / liquid outlet flow channel.

[0009] As a further optional scheme, the chip body includes base layer, intermediate layer and cover layer which are sequentially stacked;

[0010] Perforation is opened on the intermediate layer, and the base layer and cover layer are respectively arranged at both ends of the perforation, so that the perforation forms the detection cavity;

[0011] The first groove and the second groove are formed on one side of the intermediate layer close to the cover layer or one side of the cover layer close to the intermediate layer; after the intermediate layer and the cover layer are stacked, the first groove forms the liquid inlet channel and the second groove forms the liquid outlet channel.

[0012] The cover layer is provided with the liquid inlet and the liquid outlet.

[0013] As a further optional solution, the liquid inlet and the liquid outlet are both holes;

[0014] The rubber connecting piece is arranged in the liquid inlet / liquid outlet, and the rubber connecting piece is in interference fit with the liquid inlet / liquid outlet.

[0015] As a further optional solution, the liquid inlet and the liquid outlet are both formed with a first limiting step;

[0016] The rubber connecting piece comprises a first column part and a second column part which are integrally connected, the width of the second column part is greater than that of the first column part, a second limiting step is formed between the first column part and the second column part, the second limiting step abuts against the first limiting step, and the second column part is located on the side close to the intermediate layer.

[0017] As a further optional solution, at least part of the first column part protrudes from the outer surface of the cover layer.

[0018] As a further optional solution, the outer periphery profile of the first column part and / or the second column part is non-circular.

[0019] As a further optional solution, the through-hole is formed with a tapered open structure on the first column part.

[0020] As a further optional solution, the base layer, the intermediate layer and the cover layer are all transparent plate members.

[0021] As a further optional solution, the base layer, the intermediate layer and the cover layer are bonded, hot-melt connected or ultrasonically welded.

[0022] As a further optional solution, the detection cavity, the liquid inlet channel, the liquid outlet channel, the liquid inlet and the liquid outlet on the chip body are all provided with a plurality of.

[0023] Compared with the prior art, the microfluidic chip has at least the following beneficial effects:

[0024] The microfluidic chip is provided with a rubber connecting piece on the liquid inlet and the liquid outlet, the rubber connecting piece can be used for sealed docking with the injection device / liquid discharge device, the sealing property of the microfluidic chip and the injection device / liquid discharge device when they are docked is improved, and the situation of liquid leakage is avoided. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 is a structural schematic view of a microfluidic chip according to an embodiment of the present application;

[0026] Figure 2 is a structural perspective view of a microfluidic chip according to an embodiment of the present application;

[0027] Figure 3 is Figure 1 is a sectional view of A-A in Figure 1;

[0028] Figure 4 is one of the exploded schematic views of a microfluidic chip according to an embodiment of the present application;

[0029] Figure 5 is the second of the exploded schematic views of a microfluidic chip according to an embodiment of the present application;

[0030] Figure 6 is a sectional view of the cover layer and the rubber connecting piece according to an embodiment of the present application;

[0031] Figure 7 is a structural schematic view of the rubber connecting piece according to an embodiment of the present application;

[0032] Figure 8 is a structural schematic view of the intermediate layer according to another embodiment of the present application.

[0033] In the figure: 1, chip body; 1a, base layer; 1b, intermediate layer; 1c, cover layer; 11, detection cavity; 12, liquid inlet channel; 13, liquid outlet channel; 14, liquid inlet; 15, liquid outlet; 16, perforation; 17, first groove; 18, second groove; 19, first limiting step;

[0034] 2, rubber connecting piece; 21, liquid passage hole; 22, first column part; 23, second column part; 24, second limiting step. DETAILED DESCRIPTION

[0035] The specific embodiments of the present application will be further described in detail below with reference to the accompanying drawings and embodiments. The following embodiments are used to illustrate the present application, but are not used to limit the scope of the present application.

[0036] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "rear", "vertical", "horizontal" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0037] In the utility model, unless another definite provision and limitation, the terms "mount", "link", "connect", "fix" and so on should do broad sense understanding, for example, can be fixed connection, also can be detachable connection, or be integrated;Can be mechanical connection, also can be electrical connection;Can be direct connection, also can indirectly connect through intermediate medium, can be two element internal communication or two element mutual action relation. For ordinary skilled person in the art, the specific meaning of the above terms in the utility model can be understood according to specific circumstances.

[0038] In the utility model, unless another definite provision and limitation, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Moreover, the first feature "above", "over" and "on" the second feature can be directly above or obliquely above the first feature, or simply indicate that the first feature is higher than the second feature in horizontal height. The first feature "below", "under" and "under" the second feature can be directly below or obliquely below the first feature, or simply indicate that the first feature is lower than the second feature in horizontal height.

[0039] Reference Figures 1-3 An embodiment of the utility model discloses a microfluidic chip, including chip body 1 and rubber connecting piece 2, the chip body 1 is equipped with detection cavity 11, detection cavity 11 is connected with liquid inlet flow channel 12 and liquid outlet flow channel 13, liquid inlet flow channel 12 is communicated with liquid inlet 14, and liquid outlet flow channel 13 is communicated with liquid outlet 15, the rubber connecting piece 2 is arranged in liquid inlet 14 and liquid outlet 15, the inner wall of rubber connecting piece 2 and liquid inlet 14 / liquid outlet 15 are sealedly connected, and the rubber connecting piece 2 is equipped with the liquid passage 21 that communicates liquid inlet flow channel 12 / liquid outlet flow channel 13.

[0040] Specifically, the injection device / liquid discharge device (external device not shown) is docked with the rubber connecting piece 2, the rubber connecting piece 2 can be sealedly docked with the injection device / liquid discharge device, the injection device can inject liquid such as sample, reagent or sample pretreated by reagent into the liquid passage 21 of the rubber connecting piece 2, and the liquid such as sample, reagent or sample pretreated by reagent flows into the detection cavity 11 through the liquid inlet flow channel 12. The detection device can be used to count the fluorescent points of the liquid in the detection cavity 11. When the liquid in the detection cavity 11 needs to be discharged, the liquid is discharged to the liquid discharge device along the liquid outlet flow channel 13.

[0041] Wherein, the microfluidic chip is provided with rubber connecting piece 2 on liquid inlet 14 and liquid outlet 15, and the rubber connecting piece 2 can be used for sealed docking with the injection device / liquid discharge device, thereby improving the sealing performance of the microfluidic chip and the injection device / liquid discharge device when they are docked, and avoiding liquid leakage.

[0042] In some embodiments, in order to facilitate the formation of the detection cavity 11, the liquid inlet flow channel 12, the liquid outlet flow channel 13, the liquid inlet 14 and the liquid outlet 15 and other structures on the chip body 1, the chip body 1 comprises a base layer 1a, an intermediate layer 1b and a cover layer 1c which are sequentially stacked, as shown in Figure 4 and Figure 5 The intermediate layer 1b is provided with a through hole 16, and the base layer 1a and the cover layer 1c are respectively arranged at both ends of the through hole 16, so that the through hole 16 forms the detection cavity 11.

[0043] The reason for forming the detection cavity 11 by arranging the through hole 16 is to avoid forming the detection cavity 11 by integral molding. If the detection cavity 11 is formed by integral molding, it will deform during high-temperature cooling, resulting in a large structural error and a large deviation of the volume of the detection cavity 11 from the preset parameters. Therefore, in this embodiment, the base layer 1a, the intermediate layer 1b and the cover layer 1c are all transparent plates, and the through hole 16 is milled on the intermediate layer 1b, so that the detection cavity 11 formed by the through hole 16 has high structural precision.

[0044] In addition, in order to facilitate the formation of the liquid inlet flow channel 12 and the liquid outlet flow channel 13, in this embodiment, as shown in Figure 4 and Figure 5 The cover layer 1c is provided with a first groove 17 and a second groove 18 on the side close to the intermediate layer 1b. After the intermediate layer 1b and the cover layer 1c are stacked, the first groove 17 forms the liquid inlet flow channel 12, and the second groove 18 forms the liquid outlet flow channel 13.

[0045] In other embodiments, as shown in Figure 8 The intermediate layer 1b is provided with a first groove 17 and a second groove 18 on the side close to the cover layer 1c. After the intermediate layer 1b and the cover layer 1c are stacked, the first groove 17 forms the liquid inlet flow channel 12, and the second groove 18 forms the liquid outlet flow channel 13.

[0046] In addition, the cover layer 1c is provided with the liquid inlet 14 and the liquid outlet 15.

[0047] In some embodiments, in order to facilitate the sealing connection between the rubber connecting piece 2 and the inner wall of the liquid inlet 14 / liquid outlet 15, as shown in Figure 3 and Figure 6 The liquid inlet 14 and the liquid outlet 15 are both holes, and the rubber connecting piece 2 is arranged in the liquid inlet 14 / liquid outlet 15, and the rubber connecting piece 2 and the liquid inlet 14 / liquid outlet 15 are in interference fit. In this way, there is no gap between the rubber connecting piece 2 and the inner wall of the liquid inlet 14 / liquid outlet 15, avoiding liquid leakage.

[0048] Specifically, to ensure the stable installation of the rubber connecting piece 2, as shown in Figure 6 and Figure 7 , a first limiting step 19 is formed in the liquid inlet 14 and the liquid outlet 15; the rubber connecting piece 2 comprises a first column part 22 and a second column part 23 which are integrally connected, the width of the second column part 23 is greater than that of the first column part 22, a second limiting step 24 is formed between the first column part 22 and the second column part 23, the second limiting step 24 abuts against the first limiting step 19, and the second column part 23 is located on the side close to the intermediate layer 1b. Through the setting of the first limiting step 19 and the second limiting step 24, when the intermediate layer 1b and the cover layer 1c are stacked and fixed, it can be ensured that the rubber connecting piece 2 will not be separated from the cover layer 1c.

[0049] As shown in Figure 7 , the outer periphery profile of the first column part 22 and / or the second column part 23 is non-circular, so that the rubber connecting piece 2 cannot rotate in the liquid inlet 14 / liquid outlet 15, further ensuring the stable installation of the rubber connecting piece 2.

[0050] In addition, as shown in Figure 1 and Figure 3 , at least part of the first column part 22 protrudes from the outer surface of the cover layer 1c; in this way, when the rubber connecting piece 2 is docked with the injection device / liquid discharge device, the cover layer 1c will not be collided with the injection device / liquid discharge device. Further, as shown in Figure 6 , the liquid passage hole 21 forms a tapered open structure (not marked in the figure) on the first column part 22, which can facilitate the docking and insertion of the liquid injection head on the injection device, or the insertion of the liquid discharge head on the liquid discharge device.

[0051] In the above-mentioned solution, the base layer 1a, the intermediate layer 1b and the cover layer 1c are bonded, heat-welded or ultrasonically welded. In addition, as shown in Figure 2 , the detection cavity 11, the liquid inlet flow channel 12, the liquid outlet flow channel 13, the liquid inlet 14 and the liquid outlet 15 on the chip body 1 are each provided with a plurality of.

[0052] In summary, the present application provides a microfluidic chip, which is provided with a rubber connecting piece 2 on the liquid inlet 14 and the liquid outlet 15, the rubber connecting piece 2 can be used for sealed docking with the injection device / liquid discharge device, improving the sealing performance of the microfluidic chip when it is docked with the injection device / liquid discharge device, and avoiding the situation of liquid leakage.

[0053] In the above-mentioned embodiments, the description of each embodiment has its own focus, and the parts not described in detail in a certain embodiment can be referred to the relevant description of other embodiments.

[0054] The above merely is the preferred implementation form of the present application, and it should be noted that, for the ordinary skilled in the art, without departing from the technical principles of the present application, a number of improvements and substitutions can be made, and these improvements and substitutions should also be considered as the protection scope of the present application.

Claims

1. A microfluidic chip, characterized by, The chip body and the rubber connecting piece are included. A detection cavity is arranged in the chip body, and a liquid inlet channel and a liquid outlet channel are connected to the detection cavity. The rubber connecting piece is arranged at the liquid inlet and the liquid outlet, and is in sealing connection with the inner wall of the liquid inlet / liquid outlet.

2. The microfluidic chip according to claim 1, wherein: The chip body includes a base layer, an intermediate layer and a cover layer arranged in sequence. The intermediate layer is provided with a through hole, and the base layer and the cover layer are arranged at two ends of the through hole, so that the through hole forms the detection cavity. The intermediate layer is provided with a first groove and a second groove on one side close to the cover layer or on one side close to the intermediate layer of the cover layer. The cover layer is provided with the liquid inlet and the liquid outlet.

3. The microfluidic chip according to claim 2, wherein: The liquid inlet and the liquid outlet are both holes. The rubber connecting piece is arranged in the liquid inlet / liquid outlet, and the rubber connecting piece is in interference fit with the liquid inlet / liquid outlet.

4. The microfluidic chip according to claim 3, wherein: First limiting steps are formed in the liquid inlet and the liquid outlet. The rubber connecting piece includes a first column part and a second column part connected integrally, the width of the second column part is greater than that of the first column part, a second limiting step is formed between the first column part and the second column part, the second limiting step is in abutment with the first limiting step, and the second column part is located on the side close to the intermediate layer.

5. The microfluidic chip according to claim 4, wherein: At least part of the first column part protrudes from the outer surface of the cover layer.

6. The microfluidic chip according to claim 4, wherein: The outer peripheral contour of the first column part and / or the second column part is non-circular.

7. The microfluidic chip according to claim 4, wherein: The through hole forms a tapered open structure on the first column part.

8. The microfluidic chip according to claim 2, wherein: The base layer, the intermediate layer and the cover layer are all transparent plates.

9. The microfluidic chip according to claim 8, wherein: The base layer, the intermediate layer and the cover layer are connected by adhesion, hot melting or ultrasonic welding.

10. The microfluidic chip according to claim 1, wherein: The detection cavity, the liquid inlet channel, the liquid outlet channel, the liquid inlet and the liquid outlet on the chip body are all provided with multiple. ​

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