Grass carp hybridoma antibody affinity purification column

Abstract

The utility model discloses a grass carp hybridoma antibody affinity purification column which comprises an affinity purification column, the affinity purification column comprises a column body, a liquid outlet pipe and a lower cover, the liquid outlet pipe is integrally formed and connected to the lower end of the column body and communicated with the interior of the column body, and the lower cover is connected to the liquid outlet pipe in a sleeved mode; the anti-lost assembly comprises a connecting soft strip, a through hole, a clamping column and a limiting plate, one end of the connecting soft strip is movably connected to the side edge of the top of the column body, the other end of the connecting soft strip is movably connected to the lower end of the lower cover, the through hole is formed in the end, close to the lower cover, of the connecting soft strip, one end of the clamping column is fixed to the end, close to the lower cover, of the connecting soft strip, and the other end of the clamping column is inserted into the through hole; the limiting plate is fixed to the upper end of the connecting soft strip. The affinity purification column solves the problems that when an existing affinity purification column is used, a column body is fixed to a support, a lower cover and an upper cover are sequentially opened, liquid is separated out, the opened upper cover and the opened lower cover are placed on an operation table, and due to the fact that the lower cover is small and cylindrical, the lower cover is prone to being lost, and the material cost is increased.

Description

Technical Field

[0001] This utility model relates to the field of affinity purification column technology, and in particular to a grass carp hybridoma antibody affinity purification column. Background Technology

[0002] Grass carp hybridoma antibody affinity purification column is a highly efficient tool for purifying monoclonal antibodies. It mainly consists of a column body, upper cap, lower cap, and sieve plate.

[0003] In existing affinity purification columns, the column is fixed on a support, and the lower and upper caps are opened sequentially to precipitate the liquid. The opened upper and lower caps are then placed on the operating table. Because the lower cap is small and cylindrical, it is easy to lose, which increases the material cost. Therefore, there is a need to provide a grass carp hybridoma antibody affinity purification column to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a grass carp hybridoma antibody affinity purification column to solve the problem mentioned in the background art. In the use of existing affinity purification columns, the column body is fixed on a support, and the lower and upper caps are opened in sequence to precipitate liquid. The opened upper and lower caps are placed on the operating table. Since the lower cap is small and cylindrical, it is easy to lose, which increases the material cost.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0006] This utility model relates to a grass carp hybridoma antibody affinity purification column, comprising:

[0007] An affinity purification column, comprising a column body, an outlet tube, and a lower cap, wherein the outlet tube is integrally formed and connected to the lower end of the column body and communicates with the interior of the column body, and the lower cap is fitted onto the outlet tube;

[0008] An anti-loss component includes a connecting strip, a through hole, and a locking post. One end of the connecting strip is movably connected to the top side of the post, and the other end is movably connected to the lower end of the lower cover. The through hole is opened at the end of the connecting strip near the lower cover. One end of the locking post is fixed to the end of the connecting strip near the lower cover, and the other end is inserted into the through hole.

[0009] Furthermore, the anti-loss component also includes a first U-shaped connecting plate and a second U-shaped connecting plate. One end of the first U-shaped connecting plate is fixed to both sides of the top of the column, and the second U-shaped connecting plate is fixed to the middle of the lower end of the lower cover.

[0010] Furthermore, the anti-loss component also includes a limiting plate, which is fixed to one end of the connecting strip near the top of the column.

[0011] Furthermore, the upper end of the connecting strip is inserted into the inner side of the first U-shaped connecting plate, and the lower end is inserted into the inner side of the second U-shaped connecting plate.

[0012] Furthermore, the affinity purification column also includes a packing port and a top cover. The packing port is located at the top of the column and communicates with the inside of the column and the outlet pipe. The lower end of the top cover is plugged into the packing port.

[0013] Furthermore, two identical sieve plates are placed inside the column, and there is a gap between the sieve plates.

[0014] Furthermore, it also includes a blocking component, which includes a support column and a conical rubber column. The lower end of the support column is fixed to the top of the limiting plate, the conical rubber column is fixed to the top of the support column, and the lower cover is movably connected to the conical rubber column.

[0015] Compared with existing technologies, the advantages of this utility model are:

[0016] I. This utility model, through the setting of an anti-loss component, allows the connecting soft strip to pass through the inside of the first U-shaped connecting plate and then through the inside of the second U-shaped connecting plate, so that the locking post passes through the second U-shaped connecting plate and is inserted into the through hole. In use, the lower cover is removed, and the limiting plate is hung on the upper end of the first U-shaped connecting plate, so that the connecting soft strip hangs the lower cover on the side of the post. This setting can prevent the lower cover from being placed randomly after being removed, thus avoiding the occurrence of loss.

[0017] Second, with the anti-blocking component, after the lower cover is opened, it is hung on the side of the column body by the connecting soft strip. At this time, the lower cover can be held by hand, flipped over and locked downwards on the conical rubber column at the top of the support column. This setting can prevent the lower cover from hanging on the side of the column body and suspending around the liquid outlet pipe, which would affect the operation. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the affinity purification column structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the upper and lower covers of this utility model after they are opened;

[0022] Figure 4 For the present utility model Figure 1 Enlarged view of point A in the middle;

[0023] Figure 5 For the present utility model Figure 3 Enlarged view of section B in the middle.

[0024] The attached diagram lists the components represented by each number as follows:

[0025] 10. Column; 11. Packing port; 12. Liquid outlet pipe; 13. Top cover; 14. Bottom cover; 20. First U-shaped connecting plate; 21. Second U-shaped connecting plate; 22. Connecting strip; 23. Through hole; 24. Locking column; 25. Limiting plate; 30. Support column; 31. Conical rubber column. Detailed Implementation

[0026] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0027] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0028] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be described in further detail below with reference to the accompanying drawings.

[0029] Please see Figure 1-5 As shown, this embodiment is a grass carp hybridoma antibody affinity purification column, comprising:

[0030] The affinity purification column includes a column body 10, an outlet tube 12, and a lower cover 14. The outlet tube 12 is integrally formed and connected to the lower end of the column body 10 and communicates with the interior of the column body 10. The lower cover 14 is fitted onto the outlet tube 12.

[0031] The affinity purification column also includes a packing port 11 and a top cover 13. The packing port 11 is located at the top of the column body 10 and is connected to the inside of the column body 10 and the outlet pipe 12. The lower end of the top cover 13 is plugged into the packing port 11.

[0032] Inside the column 10, there are two identical sieve plates with a gap between them;

[0033] The column 10 serves as a storage container, the packing port 11 is used to place packing material and the liquid to be purified into the column 10, the liquid outlet 12 is used to discharge the liquid from the column 10, and the upper cover 13 and the lower cover 14 serve as a sealing function.

[0034] The anti-loss component includes a connecting strip 22, a through hole 23, and a locking post 24. One end of the connecting strip 22 is movably connected to the top side of the post 10, and the other end is movably connected to the lower end of the lower cover 14. The through hole 23 is opened at one end of the connecting strip 22 near the lower cover 14. One end of the locking post 24 is fixed to the end of the connecting strip 22 near the lower cover 14, and the other end is inserted into the through hole 23.

[0035] The anti-loss component also includes a first U-shaped connecting plate 20, a second U-shaped connecting plate 21, and a limiting plate 25. One end of the first U-shaped connecting plate 20 is fixed to both sides of the top of the column 10, the second U-shaped connecting plate 21 is fixed to the middle of the lower end of the lower cover 14, and the limiting plate 25 is fixed to one end of the connecting strip 22 near the top of the column 10.

[0036] The upper end of the connecting strip 22 is inserted into the inner side of the first U-shaped connecting plate 20, and the lower end is inserted into the inner side of the second U-shaped connecting plate 21;

[0037] The first U-shaped connecting plate 20 serves a connecting function, the second U-shaped connecting plate 21 serves a connecting function, the connecting strip 22 serves a connecting function, the through hole 23 serves a limiting function, the locking post 24 serves a connecting function, and the limiting plate 25 serves a limiting function.

[0038] Working principle:

[0039] The connecting strip 22 passes through the inside of the first U-shaped connecting plate 20 and then through the inside of the second U-shaped connecting plate 21, so that the locking post 24 passes through the second U-shaped connecting plate 21 and is inserted into the through hole 23. When in use, the lower cover 14 is removed and the limiting plate 25 is hung on the upper end of the first U-shaped connecting plate 20, so that the connecting strip 22 hangs the lower cover 14 on the side of the column 10.

[0040] This step prevents the lower cover 14 from being carelessly placed after being removed, thus avoiding the possibility of it being lost.

[0041] Please see Figure 1 This embodiment, based on the above embodiments, further includes:

[0042] The anti-blocking component includes a support column 30 and a conical rubber column 31. The lower end of the support column 30 is fixed to the top of the limiting plate 25, the conical rubber column 31 is fixed to the top of the support column 30, and the lower cover 14 is movably connected to the conical rubber column 31.

[0043] Support column 30 provides support, while tapered rubber column 31 provides connection.

[0044] Working principle:

[0045] After the lower cover 14 is opened, it is hung on the side of the column 10 by the connecting soft strip 22. At this time, the lower cover 14 can be held and flipped over and locked downwards onto the conical rubber column 31 at the top of the support column 30.

[0046] This step prevents the lower cover 14 from hanging on the side of the column 10 and dangling around the liquid outlet pipe 12, thus avoiding interference with operation.

[0047] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0048] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A grass carp hybridoma antibody affinity purification column, characterized in that, The application relates to an affinity purification column. The anti-lost assembly comprises a connecting soft strip (22), a through hole (23) and a clamping column (24), one end of the connecting soft strip (22) is movably connected to the top side of the column body (10), the other end is movably connected to the lower end of the lower cover (14), the through hole (23) is arranged on the end of the connecting soft strip (22) close to the lower cover (14), and one end of the clamping column (24) is fixed to the end of the connecting soft strip (22) close to the lower cover (14), and the other end is inserted into the through hole (23). The anti-lost assembly further comprises a first U-shaped connecting plate (20) and a second U-shaped connecting plate (21), one end of the first U-shaped connecting plate (20) is fixed to the top of the column body (10) on both sides, and the second U-shaped connecting plate (21) is fixed to the lower end of the lower cover (14) in the middle.

2. The grass carp hybridoma antibody affinity purification column according to claim 1, characterized in that, The anti-lost assembly further comprises a limiting plate (25), and the limiting plate (25) is fixed to one end of the connecting soft strip (22) close to the top of the column body (10).

3. The grass carp hybridoma antibody affinity purification column according to claim 1, characterized in that, The upper end of the connecting soft strip (22) is inserted into the inner side of the first U-shaped connecting plate (20), and the lower end is inserted into the inner side of the second U-shaped connecting plate (21).

4. The grass carp hybridoma antibody affinity purification column according to claim 1, characterized in that, The affinity purification column further comprises a filler port (11) and an upper cover (13), the filler port (11) is arranged at the top of the column body (10) and communicates with the interior of the column body (10) and the liquid outlet pipe (12), and the lower end of the upper cover (13) is inserted into the filler port (11).

5. The grass carp hybridoma antibody affinity purification column according to claim 1, characterized in that, Two identical sieve plates are arranged in the column body (10), and a gap exists between the sieve plates.

6. The grass carp hybridoma antibody affinity purification column according to claim 1, characterized in that, The anti-blocking assembly comprises a supporting column (30) and a conical rubber column (31), the lower end of the supporting column (30) is fixed to the top end of the limiting plate (25), the conical rubber column (31) is fixed to the top end of the supporting column (30), and the lower cover (14) is movably connected to the conical rubber column (31).

7. The grass carp hybridoma antibody affinity purification column according to claim 3, characterized in that, ​

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