Cell culture bottle
By introducing a multilayer structure into the cell culture flask, the problem of gas and nutrient transfer in the cell culture flask was solved, resulting in more efficient cell culture.
Patent Information
- Application Number
- CN202520032557.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-07
- Publication Date
- 2026-01-06
- Estimated Expiration
- 2035-01-07
AI Technical Summary
The single-layer structure of existing cell culture flasks is not conducive to gas exchange and nutrient transfer, which reduces the stability of the cell growth environment.
A multi-layered cell culture flask was designed, comprising an outer shell, an inner shell, and a circulation mechanism. The inner wall of the inner shell is provided with a flow guide groove, and the culture medium and gas are circulated through a diversion pipe and an injection valve to ensure uniform distribution of nutrients. Waste gas is discharged through an exhaust valve.
It improves the stability of the cell growth environment and culture efficiency, ensures uniform distribution of nutrients and gas exchange, reduces cell deposition, and enhances the culture effect.
Smart Images
Figure CN223766350U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, and in particular to a cell culture flask. Background Technology
[0002] Cells are the basic units of life, possessing a variety of complex structures and functions. For example, they perform protein synthesis, using messenger RNA as a template and transport RNA as a transport vehicle on ribosomes to synthesize proteins from amino acids, involving two stages: transcription and translation. Cells also transmit genetic information through DNA replication, passing it on to daughter cells. In sexual reproduction, gametes are produced through meiosis, and after fusion, they pass on the genetic information of both parents to offspring, maintaining the continuation of the species and genetic diversity. Therefore, cell culture is necessary for cell research.
[0003] Cell culture is a method that simulates the in vivo environment in vitro, enabling cells to survive, grow, reproduce, and maintain their main structures and functions. It is also an important tool for studying cell physiology, biochemical characteristics, and intercellular interactions. By culturing cancer cells, we can conduct in-depth research on the mechanisms of cancer development, including gene mutations and abnormal signal transduction pathways. Cell culture can also be used to screen and test anticancer drugs and observe the effects of drugs on cancer cell growth and apoptosis. Furthermore, it requires the use of culture flasks in conjunction with cell culture for observation and research.
[0004] Currently, cell culture flasks have relatively fixed shapes, including cylindrical and cuboid shapes. Cylindrical culture flasks have a relatively rounded curvature, while cuboid culture flasks can make better use of space and are more stable when placed. Furthermore, by changing the shape of the culture flask, such as by using a flat bottom or a U-shaped bottom, different usage needs can be met. However, even after changing the shape, the culture flask is still a single-layer structure of glass or plastic, which limits the efficiency of its internal space utilization. This makes it unfavorable for gas exchange and nutrient transfer, and reduces the stability of the cell growth environment. Utility Model Content
[0005] To overcome the above shortcomings, this invention provides a cell culture flask, which aims to improve the existing single-layer structure of culture flasks, which is not conducive to gas exchange and nutrient transfer, and reduces the stability of the cell growth environment.
[0006] To achieve the above objectives, the present invention adopts the following technical solution: a cell culture flask includes an outer shell, a threaded groove at the bottom of the outer wall of the outer shell, a fixed cap fixedly connected to the top wall of the outer shell, a bottle mouth fixedly connected to the top wall of the fixed cap, an inner shell communicating with the middle of the bottom wall of the fixed cap, a bottom shell provided at the bottom end of the outer shell, a threaded ring fixedly connected to the inner wall of the bottom shell, the bottom end of the outer wall of the outer shell being threadedly connected to the threaded groove through the outer wall of the threaded ring, and a circulation mechanism provided on the top wall of the bottle mouth, the circulation mechanism being used to improve cell culture efficiency.
[0007] As a further description of the above technical solution:
[0008] The circulation mechanism includes a mounting ring, the bottom wall of which is fixedly mounted on the top wall of the bottle opening. A diversion pipe is fixedly connected to the top wall of the mounting ring. An injection valve is connected to both the left and right sides of the diversion pipe. A guide groove is provided on the inner wall of the inner shell. A discharge valve is connected to the right side of the inner shell. The right end of the discharge valve penetrates the interior of the outer shell.
[0009] As a further description of the above technical solution:
[0010] The circulation mechanism also includes a sealing ring, the outer wall of which is fixedly connected to the inner wall of the mounting ring, and the inner wall of which is fixedly installed at the bottom of the outer wall of the diversion pipe.
[0011] As a further description of the above technical solution:
[0012] Rubber pads are fixedly installed on adjacent ends of the outer walls of the two injection valves, and the outer walls of the two rubber pads are respectively fixedly connected to the left and right sides of the diversion pipe.
[0013] As a further description of the above technical solution:
[0014] The inner bottom wall of the bottom shell is provided with a locking groove, and the bottom end of the inner shell engages with the locking groove.
[0015] As a further description of the above technical solution:
[0016] The bottom of the bottom shell is fixedly connected to a base, and the outer wall of the base is designed to be smooth.
[0017] As a further description of the above technical solution:
[0018] A locking block is fixedly connected to the rear side of the base, and a splicing groove is provided on the front side of the base. The rear side of the locking block engages with the splicing groove.
[0019] As a further description of the above technical solution:
[0020] The mounting ring has an annular groove on the outer side of its bottom wall, and the guide groove has an annular spiral design.
[0021] This utility model has the following beneficial effects:
[0022] In this invention, the use of a transparent outer shell facilitates the observation of cell growth. Meanwhile, the inner shell is fixed to the bottom of the cap, creating a multi-layered structure in the culture flask. This ensures effective isolation between the cells and the external environment, providing better gas exchange and nutrient delivery, and improving the stability of the cell growth environment.
[0023] In this invention, the guide groove on the inner wall of the inner shell allows the culture medium or gas to circulate inside the bottle. Thus, the gas or fluid injected through the two injection valves enters the interior of the inner shell through the diversion pipe, allowing nutrients or oxygen to be evenly distributed inside the inner shell. At the same time, the slow flow of air reduces cell deposition and improves culture efficiency. Attached Figure Description
[0024] Figure 1 This is a three-dimensional view of a cell culture flask proposed in this utility model;
[0025] Figure 2 This is a top view of a cell culture flask according to the present invention;
[0026] Figure 3 This is a cross-sectional view of the outer shell of a cell culture flask according to the present invention.
[0027] Figure 4 This is a split view of the inner shell of a cell culture flask according to the present invention.
[0028] Figure 5 This is an exploded view of the circulation mechanism of a cell culture flask proposed in this utility model.
[0029] Legend:
[0030] 1. Outer shell; 2. Circulation mechanism; 201. Mounting ring; 202. Diverter pipe; 203. Injection valve; 204. Guide groove; 205. Discharge valve; 206. Sealing ring; 207. Rubber pad; 3. Threaded groove; 4. Fixing cap; 5. Bottle mouth; 6. Inner shell; 7. Bottom shell; 8. Threaded ring; 9. Engaging groove; 10. Base; 11. Engaging block; 12. Assembly groove. Detailed Implementation
[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0032] Reference Figure 1 , Figure 3 and Figure 4 This utility model provides an embodiment of a cell culture flask, including an outer shell 1. The bottom of the outer wall of the outer shell 1 is provided with a threaded groove 3. A fixing cover 4 is fixedly connected to the top wall of the outer shell 1, so that the fixing cover 4 completes the fixing of the inner shell 6. The top wall of the fixing cover 4 is fixedly connected to the bottle mouth 5, which can be connected to the outer shell 1 through the threaded groove 3. With the fixing of the fixing cover 4 and the bottle mouth 5, the top of the culture flask is connected. The middle of the bottom wall of the fixing cover 4 is connected to the inner shell 6, which completes the multi-layer structure with the outer shell 1. The bottom end of the outer shell 1 is provided with a bottom shell 7, which achieves the effect of installation with the bottom of the outer shell 1. A threaded ring 8 is fixedly connected to the inner wall of the bottom shell 7, which completes the installation of the bottom of the outer shell 1, thereby achieving the purpose of opening or closing. The bottom end of the outer wall of the outer shell 1 is threadedly connected to the threaded groove 3 through the outer wall of the threaded ring 8. The top wall of the bottle mouth 5 is provided with a circulation mechanism 2, which is used to improve the cell culture efficiency.
[0033] Reference Figure 1 , Figure 3 and Figure 5 The circulation mechanism 2 includes a mounting ring 201. The bottom wall of the mounting ring 201 is fixedly installed on the top wall of the bottle mouth 5. With the connection of the mounting ring 201, it can be installed or removed from the top of the bottle mouth 5. The top wall of the mounting ring 201 is fixedly connected to a diversion pipe 202. The diversion pipe 202 allows the injection of fluid and gas. The left and right sides of the diversion pipe 202 are connected to injection valves 203. The injection valves 203 are used to transport fluid or gas. The inner wall of the inner shell 6 is provided with a guide groove 204. The guide groove 204 is used to achieve uniform distribution of nutrients or oxygen. The right side of the inner shell 6 is connected to a discharge valve 205. The discharge valve 205 is used to discharge waste gas or waste liquid. The right end of the discharge valve 205 penetrates the interior of the outer shell 1.
[0034] Reference Figure 1 , Figure 2 and Figure 5The circulation mechanism 2 also includes a sealing ring 206. The outer wall of the sealing ring 206 is fixedly connected to the inner wall of the mounting ring 201. The sealing ring 206 completes the sealing connection between the mounting ring 201 and the diversion pipe 202. The inner wall of the sealing ring 206 is fixedly installed at the bottom of the outer wall of the diversion pipe 202. The bottom of the bottom shell 7 is fixedly connected to a base 10. The base 10 improves the stability of the culture bottle. The outer wall of the base 10 has a smooth design. A locking block 11 is fixedly connected to the rear side of the base 10. A splicing groove 12 is opened on the front side of the base 10. The rear side of the locking block 11 engages with the splicing groove 12, so that the bottoms of multiple culture bottles are connected, improving the space utilization during storage.
[0035] Reference Figure 1 , Figure 4 and Figure 5 Rubber pads 207 are fixedly installed on adjacent ends of the outer walls of the two injection valves 203. The outer walls of the two rubber pads 207 are respectively fixedly connected to the left and right sides of the diversion pipe 202. This reduces wear at the connection point and improves the protection effect. The inner bottom wall of the bottom shell 7 is provided with a locking groove 9. The bottom end of the inner shell 6 engages with the locking groove 9, which ensures the stability of the inner shell 6 and improves the protection effect. The outer side of the bottom wall of the mounting ring 201 is provided with an annular groove. The guide groove 204 adopts an annular spiral design. The guide effect is improved by adopting an annular spiral design.
[0036] Working principle: The outer shell 1 of the culture flask is made of transparent material, which makes it easy for operators to observe the cell growth through the outer shell 1. At the same time, the inner shell 6 is fixed to the bottom of the fixed cap 4, so that the inside of the culture flask forms a multi-layer structure. Cells are cultured through the inner shell 6. The inner shell 6 is also made of transparent material, which ensures the isolation between the cells and the outside world, provides better gas exchange and nutrient delivery for the cells, and improves the stability of the cell growth environment.
[0037] Furthermore, the guide channel 204 on the inner wall of the inner shell 6 allows the culture medium or gas to circulate inside the inner shell 6. Thus, the gas or fluid is injected through the two injection valves 203 and enters the interior of the inner shell 6 through the diversion pipe 202, so that nutrients and oxygen can be evenly distributed inside the inner shell 6, ensuring the culture effect. Under the slow flow of air, the airflow is stirred in the fluid, thereby reducing cell deposition. Waste gas is discharged through the exhaust valve 205, which improves the culture efficiency. At the same time, the guide channel 204 completes the cleaning guidance of the inner shell 6, improving the cleaning effect.
[0038] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A cell culture flask comprising a housing (1), characterized in that: The outer wall bottom end of the shell (1) is provided with a threaded groove (3), the top wall of the shell (1) is fixedly connected with a fixed cover (4), the top wall of the fixed cover (4) is fixedly connected with a bottle mouth (5), the bottom wall of the fixed cover (4) is communicated with an inner shell (6), the bottom end of the shell (1) is provided with a bottom shell (7), the inner wall of the bottom shell (7) is fixedly connected with a threaded ring (8), the outer wall bottom end of the shell (1) is screwed with the threaded groove (3) through the outer wall of the threaded ring (8), the top wall of the bottle mouth (5) is provided with a circulating mechanism (2), and the circulating mechanism (2) is used to improve the cell culture efficiency.
2. The cell culture flask of claim 1, wherein: The circulating mechanism (2) comprises a mounting ring (201), the bottom wall of the mounting ring (201) is fixedly installed on the top wall of the bottle mouth (5), the top wall of the mounting ring (201) is fixedly connected with a shunt pipe (202), the left and right sides of the shunt pipe (202) are communicated with injection valves (203), the inner wall of the inner shell (6) is provided with a flow guide groove (204), the right side of the inner shell (6) is communicated with a discharge valve (205), and the right end of the discharge valve (205) penetrates the inside of the shell (1).
3. The cell culture flask of claim 2, wherein: The circulating mechanism (2) further comprises a sealing ring (206), the outer wall of the sealing ring (206) is fixedly connected to the inner wall of the mounting ring (201), and the inside of the sealing ring (206) is fixedly installed on the outer wall bottom end of the shunt pipe (202).
4. The cell culture flask of claim 2, wherein: The outer wall of the two injection valves (203) is fixedly installed with rubber pads (207) at adjacent ends, and the outer walls of the two rubber pads (207) are fixedly connected to the left and right sides of the shunt pipe (202) respectively.
5. The cell culture flask of claim 1, wherein: The inner bottom wall of the bottom shell (7) is provided with a clamping groove (9), and the bottom end of the inner shell (6) is clamped with the clamping groove (9).
6. The cell culture flask of claim 1, wherein: The bottom end of the bottom shell (7) is fixedly connected with a base (10), and the outer wall of the base (10) is designed to be smooth.
7. The cell culture flask of claim 6, wherein: The rear side of the base (10) is fixedly connected with a clamping block (11), the front side of the base (10) is provided with a split groove (12), and the rear side of the clamping block (11) is clamped with the split groove (12).
8. The cell culture flask of claim 2, wherein: The bottom wall outside of the mounting ring (201) is provided with a ring groove, and the outer shape of the flow guide groove (204) is designed to be a ring-shaped spiral.