Immunoassay device for stopping bleeding by biological enzyme

Abstract

The utility model discloses a biological enzyme hemostasis immunoassay device, which belongs to the field of biological enzymes and comprises an analysis system and a colorimetric component. The colorimetric assembly comprises a shell, a monochromatic light irradiation lamp group positioned at the top end of the shell, a photoelectric detector positioned at the bottom surface end of the shell and a detachable elisa plate positioned between the monochromatic light irradiation lamp group and the photoelectric detector. Compared with the prior art, the device has the advantages that the screw rod is rotated, so that the sliding block connected with the screw rod performs one-way translation along the sliding chute at the open end of the U-shaped sliding chute plate, and the longitudinal side wall of the detachable elisa plate is clamped in the translation process of the sliding block; therefore, the three side surfaces of the outer wall of the detachable elisa plate are fixed, so that the situation that the detachable elisa plate is vibrated away from the original position due to vibration during rotation of the gear transmission assembly is avoided.

Description

Technical Field

[0001] This utility model relates to the field of biological enzymes, specifically to an immunoassay device for hemostasis using biological enzymes. Background Technology

[0002] Bioenzymes are a class of proteins with biocatalytic functions. They can accelerate the rate of chemical reactions in living organisms without being consumed in the process. In industry and medicine, bioenzymes are widely used in food processing, detergent production, drug synthesis, disease diagnosis and treatment, and more. With ongoing research into the medical applications of bioenzymes, broad-spectrum bioenzyme sterilizers are on the horizon. Experiments have shown that for small-area wounds, a simple spray application can quickly disinfect and stop bleeding, resulting in good healing. Further research is needed to analyze the specific properties of this bioenzyme. To determine the suitability and cultivation protocol for this hemostatic enzyme, a routine analytical procedure is required. This procedure includes sampling, enzymatic analysis, reaction equilibrium, product inhibition, reaction rate method, and data analysis. Enzymatic analysis uses enzymatic reactions to determine substances that are difficult to detect in the sample. After the enzyme has been cultured and developed in a directional manner at reaction equilibrium, the amount of the product is determined by colorimetry and electrophoresis, thereby calculating the concentration of the analyte. Colorimetry is widely used in the immunoassay of enzymes (such as in commonly used microplate readers) due to its simplicity and low cost.

[0003] The existing immunoassay device for bio-enzyme hemostasis consists of an analysis system and a colorimetric component. The detachable enzyme-labeled plate containing the bio-enzyme is inserted into the groove of the moving module through the through-hole on the side of the colorimetric component housing. The moving module is retracted into the top of the fixed module via a gear transmission component. The analysis system selects the corresponding light illumination switch to activate the corresponding monochromatic light illumination lamp group of the colorimetric component. The light emitted by the monochromatic light illumination lamp group passes through the bio-enzyme, and the photodetector below the detachable enzyme-labeled plate receives the corresponding light signal. After conversion by the photodetector, it is converted into an electrical signal that can be recognized by the analysis system. Finally, the analysis system amplifies and filters the electrical signal and converts it into data for display.

[0004] The number of enzymes required varies in different bio-enzyme analysis processes. Therefore, the number and size of detachable ELISA plates also vary. As a result, detachable ELISA plates have different specifications. This may cause them to be loosely fixed when placed in the moving module, resulting in positional displacement due to vibration during gear transmission. Consequently, the photodetector may fail to detect normal data, thus affecting the final data results. Utility Model Content

[0005] The technical problem this invention aims to solve is that, when the detachable enzyme-labeled plate of the existing bio-enzyme hemostasis immunoassay device is placed into the moving module, its shape may not match, resulting in positional deviation during transmission and affecting the detection results of the photoelectric detector.

[0006] To solve the above-mentioned technical problems, the technical solution provided by this utility model is as follows: an immunoassay device for biological enzyme hemostasis, comprising an analysis system and a colorimetric component. The colorimetric component includes a housing, a monochromatic light irradiation lamp group located at the top of the housing, a photodetector located at the bottom of the housing, and a detachable enzyme-labeled plate located between the monochromatic light irradiation lamp group and the photodetector. The detachable enzyme-labeled plate reciprocates within the housing via a gear transmission assembly. The gear transmission assembly includes a fixed module and a moving module. The moving module passes through the side wall of the housing. The interior of the moving module is provided with a longitudinal width adjustment mechanism adapted to detachable enzyme-labeled plates of different sizes. The longitudinal width adjustment mechanism includes a U-shaped sliding groove plate that slides along the direction of the fixed module, a sliding block that passes through the open end side wall of the U-shaped sliding groove plate, and a spiral rod and an auxiliary support rod that pass through the two side walls of the sliding block respectively.

[0007] As an improvement, both sides of the sliding block are provided with H-shaped grooves to limit the offset of the sliding block, and the open end of the sliding block groove of the U-shaped sliding groove plate is provided with a sealing plate connected by a pin. The spiral rod and the auxiliary support rod both pass through the sealing plate.

[0008] As an improvement, the fixed module includes a fixed convex sliding groove located on the inner wall of the housing, a drive gear located on one side of the outer wall of the convex sliding groove, and a servo motor driving the drive gear. A ruler strip that meshes with the drive gear is provided on one side of the outer wall of the U-shaped sliding groove plate, and the ruler strip passes through the side wall of the fixed convex sliding groove.

[0009] As an improvement, a dust cover is provided inside the moving module through hole on the side wall of the housing, and the dust cover is connected to the outer wall of the housing through an automatic rebound assembly.

[0010] As an improvement, the analysis system includes a lighting control system for adjusting the number and position of the monochromatic light irradiation lamps, a detachable ELISA plate handling system for controlling the start and stop of the gear transmission assembly, and a data analysis system for receiving electrical signals from a photodetector.

[0011] As an improvement, the monochromatic light illumination lamp group includes several light sources arranged in an array and a filter plate located at the lamp opening of the light source.

[0012] The advantages of this invention compared with the prior art are as follows: This device rotates the screw rod, causing the sliding block connected to it to move unidirectionally along the open end of the U-shaped sliding groove plate. During the sliding block's translation, the longitudinal sidewall of the detachable enzyme labeling plate is clamped, thereby fixing the three sides of the outer wall of the detachable enzyme labeling plate. This ensures that the detachable enzyme labeling plate will not be shaken off its original position due to vibration when the gear transmission assembly rotates. Attached Figure Description

[0013] Figure 1 This is a general structural diagram of an immunoassay device for bio-enzyme hemostasis according to the present invention.

[0014] Figure 2 This is a structural diagram of the gear transmission component of an immunoassay device for bio-enzyme hemostasis according to this utility model.

[0015] Figure 3 This is a structural diagram of a modular immunoassay device for hemostasis based on biological enzymes, according to this utility model.

[0016] Figure 4 This is an exploded view of the longitudinal width adjustment mechanism of an immunoassay device for bio-enzyme hemostasis according to this utility model.

[0017] Figure 5 This is a structural diagram of the sliding block of an immunoassay device for bio-enzyme hemostasis according to this utility model.

[0018] Figure 6 This is a general sectional view of an immunoassay device for hemostasis using biological enzymes, according to this utility model.

[0019] Figure 7 This is a structural diagram of the monochromatic light irradiation lamp group of an immunoassay device for bio-enzyme hemostasis according to this utility model.

[0020] As shown in the figure: 1. Analysis system; 2. Colorimetric component; 21. Housing; 211. Dust cover; 22. Monochromatic light irradiation lamp assembly; 221. Light source lamp; 222. Filter plate; 23. Photodetector; 24. Detachable enzyme-labeled plate; 3. Gear transmission assembly; 31. Fixed module; 311. Fixed convex slide groove; 312. Drive gear; 313. Servo motor; 314. Ruler strip; 32. Moving module; 4. Longitudinal width adjustment mechanism; 41. U-shaped sliding groove plate; 42. Sliding block; 421. H-shaped slot; 43. Spiral rod; 44. Auxiliary support rod; 45. Sealing plate; 5. Automatic rebound assembly. Detailed Implementation

[0021] The present invention will now be described in further detail with reference to the accompanying drawings.

[0022] As per the instruction manual Figure 1 , 6As shown in Figure 7, the system includes an analysis system 1 and a colorimetric component 2. The colorimetric component 2 includes a housing 21, a monochromatic light irradiation lamp group 22 located at the top of the housing 21, a photodetector 23 located at the bottom of the housing 21, and a detachable enzyme-linked immunosorbent assay (ELISA) plate 24 located between the monochromatic light irradiation lamp group 22 and the photodetector 23. The monochromatic light irradiation lamp group 22 includes several light source lamps 221 arranged in an array and a filter plate 222 located at the lamp opening of the light source lamps 221. The light source lamps 221 are arranged in an array according to the spacing of the detachable ELISA plate 24, and a fixed... The fixing plate secures the light sources 221, and the wires connecting the light sources 221 are connected in parallel to the inlet of the fixing plate. The filter lens of the filter plate 222 is aligned with the lamp port of the light source 221. The fixing plate and the filter plate 222 are fixedly connected with screws, and the fixing plate is fixed to the top of the inside of the housing 21 with screws. The photodetector 23 is placed directly below the filter plate 222, and the position of the light receiving port of the photodetector 23 is aligned with the position of the filter lens. The photodetector 23 is fixed to the bottom surface of the housing 21 with screws.

[0023] The analysis system 1 includes a lighting control system for adjusting the number and position of the monochromatic light irradiation lamps 22, a detachable ELISA plate handling system for controlling the start and stop of the gear transmission assembly 3, and a data analysis system for receiving electrical signals from the photodetector 23. The lighting control system controls the activation of each light source lamp 221. These circuits are controlled by the PLC control board of the lighting control system (the lighting control system, data analysis system, and detachable ELISA plate handling system are all common basic systems that come with existing ELISA readers).

[0024] As per the instruction manual Figure 1 , 2 As shown in Figures 3, 4, and 5, the detachable enzyme-labeled plate 24 reciprocates within the housing 21 via a gear transmission assembly 3. The gear transmission assembly 3 includes a fixed module 31 and a moving module 32. The moving module 32 passes through the side wall of the housing 21. A dust cover 211 is provided inside the through hole of the moving module 32 on the side wall of the housing 21. The dust cover 211 is connected to the outer wall of the housing 21 via an automatic spring-loaded assembly 5. The dust cover 211 is fixed to the through hole of the moving module 32 in the housing 21 using the automatic spring-loaded assembly 5. This ensures that the moving module 32 can move in and out normally while preventing dust from entering the machine when it is not in use. The dust cover 211 is opened by contact with the moving module 32. Under normal conditions, the dust cover 211 automatically closes under the action of the automatic spring-loaded assembly 5, preventing the through hole of the moving module 32 from being forgotten to be closed.

[0025] The moving module 32 is internally equipped with a longitudinal width adjustment mechanism 4 to accommodate detachable enzyme-labeled plates 24 of different sizes. The longitudinal width adjustment mechanism 4 includes a U-shaped sliding groove plate 41 that slides along the direction of the fixed module 31, a sliding block 42 passing through the open end sidewall of the U-shaped sliding groove plate 41, and a spiral rod 43 and an auxiliary support rod 44 passing through the two sidewalls of the sliding block 42 respectively. Both sides of the sliding block 42 are provided with H-shaped slots 421 to limit its offset. The open end of the sliding block 42 of the U-shaped sliding groove plate 41 is provided with a sealing plate 45 connected by a pin. The spiral rod 43 and the auxiliary support rod 44 both pass through the sealing plate 45. The opening of the U-shaped sliding groove plate 41... The inner wall of the end slide groove is provided with a groove corresponding to the H-shaped groove 421 to limit the displacement of the side of the sliding block 42. The spiral rod 43 and the auxiliary support rod 44 are screwed into the screw holes and through holes on both sides of the sliding block 42, respectively, and the sliding block 42 is inserted into the open end slide groove of the U-shaped slide groove plate 41. The bottom end of the spiral rod 43 and the auxiliary support rod 44 passes through the bottom side wall of the open slide groove of the U-shaped slide groove plate 41. The other end of the spiral rod 43 and the auxiliary support rod 44 passes through the central through holes of the two sealing plates 45, respectively, and the two sealing plates 45 are sealed to the open end of the U-shaped slide groove plate 41 with pins. Finally, a rotating handle is installed on the side shaft of the sealing plate 45 of the spiral rod 43.

[0026] The fixed module 31 includes a fixed convex sliding groove 311 located on the inner wall of the housing 21, a drive gear 312 located on one side of the outer wall of the convex sliding groove 311, and a servo motor 313 driving the drive gear 312. A ruler strip 314 meshing with the drive gear 312 is provided on one side of the outer wall of the U-shaped sliding groove plate 41. The ruler strip 314 passes through the side wall of the fixed convex sliding groove 311, sliding the U-shaped sliding groove plate 41 containing the ruler strip 314 into the inner wall of the fixed convex sliding groove 311. A sliding groove corresponding to the protruding end of the fixed convex sliding groove 311 is provided on the outer wall of the U-shaped sliding groove plate 41. During sliding, the protruding end of the fixed convex sliding groove 311 is engaged with the U-shaped sliding groove plate 41. In the sliding groove of 1, the ruler strip 314 passes through the through groove of the ruler strip 314 on one side of the fixed convex sliding groove 311. The output shaft of the servo motor 313 is fixed to the center through hole of the drive gear 312 using a connecting key. The drive gear 312 is placed on the ruler strip 314. The servo motor 313 is fixed to the fixing block on the outer wall of the fixed convex sliding groove 311 using screws. Finally, the fixed convex sliding groove 311 is fixed to the inner wall of the housing 21 using screws. Note that the zero point position of the U-shaped sliding groove plate 41 is aligned with the zero point position of the monochromatic light irradiation lamp group 22 to ensure that when the detachable enzyme label plate 24 is placed, the monochromatic light can accurately irradiate the liquid storage tank of the detachable enzyme label plate 24.

[0027] In practical implementation, clicking the detachable ELISA plate loading and unloading system causes the servo motor 313 to reverse, driving the ruler strip 314 to move towards the opening of the moving module on the side of the housing 21 via the drive gear 312. During the movement, the U-shaped sliding groove plate 41 pushes open the dust cover 211. After the U-shaped sliding groove plate 41 moves out, the detachable ELISA plate 24 is placed on one side corner of the U-shaped sliding groove plate 41. Rotating the handle of the screw rod 43 causes the sliding block 42 to move onto the side wall of the detachable ELISA plate 24 (both the U-shaped sliding groove plate 41 and the sliding block 42 are L-shaped stepped plates, and the detachable ELISA plate 24...). 4. Place the plate at the inner right angle of the L-shaped stepped plate. After clamping, activate the retrieval function of the detachable ELISA plate handling system to move the detachable ELISA plate 24 to below the lamp opening of the lower filter plate 222. Select the location of the biological enzyme through the light control system and activate the monochromatic light at the corresponding location. Under the illumination of the monochromatic light, part of the monochromatic light will be absorbed by the biological enzyme, while the other part of the monochromatic light will pass through the biological enzyme and illuminate the photodetector 23 below the detachable ELISA plate 24. The photodetector 23 converts the received light signal into an electrical signal and then transmits the electrical signal to the data analysis system for analysis.

[0028] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit of the present invention, such designs should fall within the protection scope of the present invention.

Claims

1. An immunoassay device for bio-enzyme hemostasis, comprising an analysis system (1) and a colorimetric component (2), wherein the colorimetric component (2) comprises a housing (21), a monochromatic light irradiation lamp group (22) located at the top of the housing (21), a photodetector (23) located at the bottom of the housing (21), and a detachable enzyme-labeled plate (24) located between the monochromatic light irradiation lamp group (22) and the photodetector (23), wherein the detachable enzyme-labeled plate (24) reciprocates within the housing (21) via a gear transmission assembly (3), wherein the gear transmission assembly (3) comprises a fixed module (31) and a moving module (32), the moving module (32) passing through the side wall of the housing (21), characterized in that: The moving module (32) is equipped with a longitudinal width adjustment mechanism (4) to adapt to detachable enzyme labeling plates (24) of different sizes. The longitudinal width adjustment mechanism (4) includes a U-shaped sliding groove plate (41) that slides along the direction of the fixed module (31), a sliding block (42) that passes through the side wall of the open end of the U-shaped sliding groove plate (41), and a spiral rod (43) and an auxiliary support rod (44) that pass through the side walls of the sliding block (42) respectively.

2. The immunoassay device for bio-enzyme hemostasis according to claim 1, characterized in that: Both sides of the sliding block (42) are provided with H-shaped slots (421) to limit the offset of the sliding block (42). The open end of the sliding block (42) of the U-shaped sliding groove plate (41) is provided with a sealing plate (45) connected by a pin. The spiral rod (43) and the auxiliary support rod (44) both pass through the sealing plate (45).

3. The immunoassay device for bio-enzyme hemostasis according to claim 1, characterized in that: The fixed module (31) includes a fixed convex slide groove (311) located on the inner wall of the housing (21), a drive gear (312) located on one side of the outer wall of the convex slide groove (311), and a servo motor (313) driving the drive gear (312). A ruler strip (314) that meshes with the drive gear (312) is provided on one side of the outer wall of the U-shaped sliding groove plate (41). The ruler strip (314) passes through the side wall of the fixed convex slide groove (311).

4. The immunoassay device for bio-enzyme hemostasis according to claim 1, characterized in that: A dust cover (211) is provided inside the through hole of the moving module (32) on the side wall of the housing (21). The dust cover (211) is connected to the outer wall of the housing (21) through the automatic rebound assembly (5).

5. The immunoassay device for bio-enzyme hemostasis according to claim 1, characterized in that: The analysis system (1) includes a lighting control system for adjusting the number and position of the monochromatic light irradiation lamp group (22), a detachable enzyme-labeled plate picking and placing system for controlling the start and stop of the gear transmission assembly (3), and a data analysis system for receiving electrical signals from the photodetector (23).

6. The immunoassay device for bio-enzyme hemostasis according to claim 1, characterized in that: The monochromatic light illumination lamp group (22) includes a number of light source lamps (221) arranged in an array and a filter plate (222) located at the lamp opening of the light source lamps (221).

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