Pipetting mechanism and analyzer

Abstract

The utility model discloses a pipetting mechanism and an analyzer, the pipetting mechanism comprises a loading support, a loading piece, a first elastic piece and a second elastic piece, the loading piece is connected with the loading support in a sliding manner and is used for loading a pipetting head; the first elastic piece can deform along with sliding of the loading piece relative to the loading support in the second direction after the loading piece makes contact with the pipetting head, and the second elastic piece can deform along with continuous sliding of the loading piece relative to the loading support in the second direction after the first elastic piece reaches a first preset deformation amount. In the loading process of the pipetting head, the first elastic piece provides the loading acting force, and after the first elastic piece deforms to the first preset deformation amount, the first elastic piece and the second elastic piece jointly provide the loading acting force, so that the pipetting head is stably loaded on the loading piece, and the risk of pipetting failure caused by looseness of the pipetting head in the pipetting process is reduced; and the loading reliability and the sealing performance of the pipetting head can be improved.

Description

Technical Field

[0001] This utility model relates to the field of pipetting structure technology, and in particular to a pipetting mechanism and analyzer. Background Technology

[0002] A pipetting mechanism typically includes a loading component for mounting pipette tips. Using this mechanism, the sample or reagent contained within the pipette tip can be transferred to the appropriate location for testing. In related technologies, during pipette tip loading, the loading component is inserted into the pipette tip, causing the pipette tip to deform and complete the loading. However, because the deformation of different pipette tips is inconsistent, and the deformation of the same pipette tip after multiple uses is uncontrollable, it is easy for the pipette tip to become loose or even fall off during pipetting due to incomplete loading. This reduces the reliability and sealing of the pipette tip loading, easily leading to pipetting failure or inaccurate pipetting volume. Summary of the Invention

[0003] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention proposes a pipetting mechanism that improves the reliability and sealing of pipetting head loading.

[0004] This invention also proposes an analyzer having the above-mentioned pipetting mechanism.

[0005] The pipetting mechanism according to a first aspect embodiment of the present invention includes:

[0006] Loading bracket;

[0007] A loading member, slidably connected to the loading bracket and used for loading a pipette tip, is configured to: follow the loading bracket in a first direction to move closer to the pipette tip, and slide relative to the loading bracket in a second direction after contacting the pipette tip, wherein the first direction is opposite to the second direction;

[0008] A first elastic element is disposed between the loading member and the loading bracket, and is capable of deforming along the second direction after the loading member contacts the pipette head and slides relative to the loading bracket, so as to provide a force to the loading member in the first direction;

[0009] The second elastic element is disposed between the loading member and the loading bracket. The second elastic element can deform as the loading member continues to slide relative to the loading bracket in the second direction after the first elastic element reaches a first preset deformation amount, and provide a force to the loading member in the first direction.

[0010] The pipetting mechanism according to the embodiments of the present invention has at least the following beneficial effects:

[0011] In the embodiments of this utility model, during the loading process of the pipette tip, the elastic force generated by the deformation of the first elastic element first provides the loading force of the pipette tip onto the loading component. After the first elastic element deforms to the first preset deformation amount, the second elastic element begins to deform. Thereafter, the first elastic element and the second elastic element jointly provide the loading force of the pipette tip onto the loading component. The pipetting mechanism can provide sufficient loading force for the loading of the pipette tip, so that the pipette tip is stably loaded onto the loading component, reducing the risk of pipetting failure caused by loosening of the pipette tip during the pipetting process, and improving the reliability and sealing of the pipette tip loading.

[0012] According to some embodiments of the present invention, the pipetting mechanism further includes a driving module, which is connected to the loading bracket and is used to drive the loading bracket to move along the first direction or the second direction. The driving module is configured to stop driving the loading bracket to move along the first direction when the first elastic element reaches a second preset deformation amount or the second elastic element reaches a third preset deformation amount, wherein the second preset deformation amount is greater than the first preset deformation amount.

[0013] According to some embodiments of the present invention, the pipetting mechanism further includes a first sensing element and a first triggering element. The first sensing element is connected to the drive module, and the first triggering element is connected to the loading bracket. The first triggering element moves synchronously with the loading bracket. The drive module is configured such that the first sensing element is triggered by the first triggering element when the first elastic element reaches the second preset deformation amount or the second elastic element reaches the third preset deformation amount. The drive module stops driving the loading bracket to move according to the triggering signal of the first sensing element.

[0014] Alternatively, the pipetting mechanism further includes a second sensing element and a second triggering element. The second sensing element is connected to the loading bracket, and the second triggering element is connected to the loading member. The second triggering element moves synchronously with the loading member. The drive module is configured such that the second sensing element is triggered by the second triggering element when the first elastic member reaches the second preset deformation amount or the second elastic member reaches the third preset deformation amount. The drive module stops driving the loading bracket to move according to the triggering signal of the second sensing element.

[0015] Alternatively, the pipetting mechanism may further include a pressure detection element connected to the loading bracket. The pressure detection element is used to detect the pressure applied to the loading bracket by the first elastic element or the pressure applied to the loading bracket by the second elastic element. The drive module is configured to stop driving the loading bracket to move when the detection value of the pressure detection element reaches a preset pressure range.

[0016] According to some embodiments of the present invention, the pipetting mechanism includes a movable seat, the loading member has a sliding portion and a supporting portion disposed around the sliding portion, the sliding portion has a free end for loading the pipetting head, the movable seat is slidably connected to the sliding portion, one end of the free end of the second elastic member facing away from the sliding portion is connected to the loading bracket, and one end of the free end of the second elastic member facing the sliding portion is connected to the movable seat, the loading member is configured such that: after the first elastic member reaches the first preset deformation amount, the supporting portion abuts against the movable seat and pushes the second elastic member to deform;

[0017] Alternatively, the loading member has a sliding portion and a supporting portion disposed around the sliding portion. The sliding portion is slidably connected to the loading bracket, and the sliding portion has a free end for loading the pipette tip. One end of the second elastic member facing the free end of the sliding portion is connected to the loading bracket, and the other end of the second elastic member facing the free end of the sliding portion is suspended. The loading member is configured such that after the first elastic member reaches the first preset deformation amount, the supporting portion abuts against the suspended end of the second elastic member and pushes the second elastic member to deform.

[0018] According to some embodiments of the present invention, the pipetting mechanism includes a first pre-tightening seat and a second pre-tightening seat. The first pre-tightening seat is connected to the loading member, and the second pre-tightening seat is connected to the loading bracket. The two ends of the first elastic member are respectively connected to the first pre-tightening seat and the second pre-tightening seat. The first pre-tightening seat and / or the second pre-tightening seat can move along the first direction or the second direction to adjust the pre-tightening amount of the first elastic member.

[0019] And / or, the pipetting mechanism includes a third pre-tightening seat and a fourth pre-tightening seat, the third pre-tightening seat being connected to the loading member, the fourth pre-tightening seat being connected to the loading bracket, the two ends of the second elastic member being respectively connected to the third pre-tightening seat and the fourth pre-tightening seat, the third pre-tightening seat and / or the fourth pre-tightening seat being movable along the first direction or the second direction to adjust the pre-tightening amount of the second elastic member.

[0020] According to some embodiments of this utility model, before the loading member contacts the pipette head, the first elastic member has a first deformation amount L. 11 After the pipette head is loaded onto the loading member, the first elastic member has a second deformation amount L. 12 The original length of the first elastic element is L. 10 Where 0.8≤L 11 / L 10 ≤1, 0.5≤L 12 / L10 ≤0.8; Before the loading component contacts the pipette head, the second elastic element has a third deformation amount L. 21 After the pipette head is loaded onto the loading member, the second elastic member has a fourth deformation amount L. 22 The original length of the second elastic element is L. 20 Where 0.9≤L 21 / L 20 ≤1, 0.7≤L 22 / L 20 ≤0.9;

[0021] And / or, the stiffness of the first elastic element is k1, the stiffness of the second elastic element is k2, 0.56N / mm≤k1≤0.84N / mm, 4.64N / mm≤k2≤6.96N / mm.

[0022] According to some embodiments of the present invention, the first elastic member has a first end and a second end disposed along the first direction, the second end being closer than the first end to the end of the loading member used for loading the pipette tip; wherein, the first end is connected to the loading bracket, the second end is connected to the loading member, and after the loading member contacts the pipette tip, the first elastic member is compressed following the sliding of the loading member; or, the first end is connected to the loading member, the second end is connected to the loading bracket, and after the loading member contacts the pipette tip, the first elastic member is stretched following the sliding of the loading member;

[0023] The second elastic member has a third end and a fourth end disposed along the first direction, the fourth end being closer than the third end to the end of the loading member used for loading the pipette tip; wherein the third end is connected to the loading bracket, the fourth end is connected to the loading member, and after the first elastic member reaches the first preset deformation amount, the second elastic member is compressed as the loading member slides; or, the third end is connected to the loading member, the fourth end is connected to the loading bracket, and after the first elastic member reaches the first preset deformation amount, the second elastic member is stretched as the loading member slides.

[0024] According to some embodiments of the present invention, the arrangement direction of the first elastic member and the second elastic member is parallel to the sliding direction of the loading member;

[0025] Alternatively, the arrangement direction of the first elastic member and the second elastic member intersects the sliding direction of the loading member. The first elastic member has a first end and a second end opposite to each other, and the second elastic member has a third end and a fourth end opposite to each other. The end of the first elastic member facing the loading member for loading the pipette tip is the second end, and the end of the second elastic member facing the loading member for loading the pipette tip is the fourth end. The second end is closer to the loading member end for loading the pipette tip than the fourth end.

[0026] Alternatively, the second elastic element is sleeved outside the first elastic element. The first elastic element has a first end and a second end opposite to each other, and the second elastic element has a third end and a fourth end opposite to each other. The end of the first elastic element facing the end of the loading member used to load the pipette tip is the second end, and the end of the second elastic element facing the end of the loading member used to load the pipette tip is the fourth end. The second end is closer to the end of the loading member used to load the pipette tip than the fourth end.

[0027] According to some embodiments of the present invention, the pipetting mechanism further includes a third sensing element and a third triggering element. The third sensing element is connected to the loading bracket, and the third triggering element is connected to the loading component and can move synchronously with the loading component. The third sensing element is configured to: be in a signal shielding state during the loading of the pipetting head onto the loading component; be in a signal acquisition state after the pipetting head is loaded onto the loading component; and be triggered by the third triggering element to issue an alarm signal when the first elastic element reaches a fourth preset deformation amount, wherein the fourth preset deformation amount is less than the first preset deformation amount.

[0028] Alternatively, the third sensing element is configured to: be in a stopped working state during the loading of the pipette head onto the loading component, be in a working state after the pipette head is loaded onto the loading component, and be triggered by the third triggering element to issue an alarm signal when the first elastic element reaches a fourth preset deformation amount, wherein the fourth preset deformation amount is less than the first preset deformation amount.

[0029] The analyzer according to a second aspect embodiment of the present invention includes:

[0030] The nucleic acid extraction module is used to extract nucleic acids from samples to obtain nucleic acid extract.

[0031] The pipetting mechanism in the first aspect embodiment is at least used to transfer the nucleic acid extract into the amplification container;

[0032] An amplification module is used to amplify the nucleic acid extract loaded in the amplification container to obtain a test solution;

[0033] The detection module is used to detect the liquid to be tested.

[0034] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0035] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0036] Figure 1 This is a schematic diagram of one embodiment of the pipetting mechanism of this utility model;

[0037] Figure 2 for Figure 1 A cross-sectional view of an embodiment of a central pipetting mechanism;

[0038] Figure 3 This is a schematic diagram showing the positional arrangement of the various sensing elements in the pipetting mechanism;

[0039] Figure 4 This is a schematic diagram showing the distribution of each pretensioner seat in one embodiment;

[0040] Figure 5 This is a schematic diagram showing the distribution of each pretensioner seat in another embodiment;

[0041] Figure 6 This is a schematic diagram showing the arrangement of the first elastic element and the second elastic element in one embodiment;

[0042] Figure 7 This is a schematic diagram showing the arrangement of the first elastic element and the second elastic element in another embodiment;

[0043] Figure 8 This is a schematic diagram showing the positional engagement of the second elastic member and the supporting part in one embodiment.

[0044] Figure label:

[0045] Pipette 10; First sensing element 101, first triggering element 102, second sensing element 103, second triggering element 104, pressure detection element 105, first pre-tightening seat 106, second pre-tightening seat 107, third pre-tightening seat 108, fourth pre-tightening seat 109, adjusting seat 110, movable seat 111, fixed seat 112, pushing seat 113, third sensing element 114, third triggering element 115; loading bracket 100, mounting groove 120, guide channel 130, second guide part 140; loading component 200, cavity 210, pushing part 220, sliding part 230, free end 231, supporting part 240, first guide part 250; first elastic element 300, first end 310, second end 320; second elastic element 400, third end 410, fourth end 420; drive module 500. Detailed Implementation

[0046] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0047] In the description of this utility model, it should be understood that the directional descriptions, such as up, down, front, back, left, right, etc., indicate the directional or positional relationship based on the directional or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0048] In the description of this utility model, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0049] In the description of this utility model, unless otherwise explicitly defined, terms such as "setting," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this utility model in conjunction with the specific content of the technical solution.

[0050] In the description of this utility model, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this utility model. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0051] This utility model provides a pipetting mechanism. After being equipped with a pipette tip 10, the pipetting mechanism can perform pipetting operations on the sample or reagent being tested. The pipette tip 10 can safely and quantitatively transfer samples or reagents, preventing cross-contamination. The pipette tip 10 is not limited to being a tip, injection needle, etc. (See reference...) Figure 1 In some embodiments, the pipetting mechanism includes a loading bracket 100, a loading member 200, a first elastic member 300, and a second elastic member 400. One end of the loading member 200 is used to load the pipetting head 10. The loading member 200 is slidably connected to the loading bracket 100. The loading member 200 can move synchronously with the loading bracket 100 and can slide relative to the loading bracket 100. The first elastic member 300 and the second elastic member 400 are configured as elastic components that can be driven to produce elastic deformation and can automatically recover, such as springs, sheet metal, etc.

[0052] Both the first elastic element 300 and the second elastic element 400 are disposed between the loading member 200 and the loading bracket 100. When the loading member 200 and the loading bracket 100 slide relative to each other, the first elastic element 300 and the second elastic element 400 can deform following the sliding of the loading member 200. Specifically, during the loading process of the loading member 200 loading the pipette head 10, the loading member 200 moves along the first direction with the loading bracket 100 to approach the pipette head 10. When the loading member 200 contacts the pipette head 10, the loading member 200 is subjected to the force applied by the pipette head 10 and slides relative to the loading bracket 100 along the second direction. The first direction is opposite to the second direction. The first elastic element 300 deforms along with the sliding of the loading member 200 relative to the loading bracket 100 in the second direction and provides a force towards the loading member 200 in the first direction. The force enables the pipette head 10 to be flexibly loaded onto the loading member 200. When the first elastic member 300 reaches the first preset deformation amount, the second elastic member 400 deforms as the loading member 200 continues to slide relative to the loading bracket 100 in the second direction, and provides a force to the loading member 200 in the first direction. At this time, the elastic forces generated by the first elastic member 300 and the second elastic member 400 are in the same direction, and together provide the force required for the loading member 200 to load the pipette head 10 until the pipette head 10 is fully loaded.

[0053] In the initial contact between the pipette tip 10 and the loading member 200, the elastic force generated by the deformation of the first elastic member 300 provides the loading force of the pipette tip 10 onto the loading member 200. After the first elastic member 300 deforms to a first preset deformation amount, the second elastic member 400 begins to deform and applies an elastic force to the loading member 200. Thereafter, the first elastic member 300 and the second elastic member 400 jointly provide the loading force of the pipette tip 10 onto the loading member 200. During the loading process of the pipette tip 10, as the second elastic member 400 begins to deform after the first elastic member 300 deforms to the first preset deformation amount, and as the deformation amounts of the first elastic member 300 and the second elastic member 400 gradually increase, the loading force provided by the first elastic member 300 and the second elastic member 400 gradually increases. The pipetting mechanism can provide sufficient loading force for the loading of the pipette tip 10, so that the pipette tip 10 is stably loaded onto the loading member 200, reducing the risk of pipetting failure caused by loosening of the pipette tip 10 during the pipetting process, and improving the reliability and sealing of the loading of the pipette tip 10.

[0054] It should be noted that during the loading process of the pipette head 10, the loading bracket 100 always drives the loading component 200 to move along the first direction. When the loading component 200 moves along the first direction and comes into contact with the pipette head 10, the loading component 200 will continue to move along the first direction and will be resisted by the pipette head 10. Under the action of this resistance, the loading component 200 slides relative to the loading bracket 100 along the second direction until the pipette head 10 is fully loaded, so that the first elastic element 300 and the second elastic element 400 continuously deform during the loading process of the pipette head 10 and provide a gradually increasing loading force.

[0055] In one embodiment, both the first direction and the second direction are set to vertical, with the first direction vertically downward and the second direction vertically upward. Before loading, the pipette head 10 is located below the loading member 200. After loading begins, the loading member 200 moves downward with the loading support 100 and gradually approaches the pipette head 10. After the lower end of the loading member 200 contacts the pipette head 10, it is subjected to an upward force applied by the pipette head 10 and slides upward relative to the loading support 100, causing the first elastic member 300 to deform. The first elastic member 300 provides a downward force to the loading member 200. After the first elastic member 300 reaches a first preset deformation amount, the second elastic member 400 follows the loading member 200 to slide upward relative to the loading support 100 and deforms, providing a downward force to the loading member 200 until the pipette head 10 is fully loaded.

[0056] Understandably, after the pipette tip 10 is mounted on the loading unit 200, the pipetting mechanism can further perform aspiration and dispensing operations. Specifically, refer to... Figure 2The loading component 200 has a through cavity 210 inside. After the pipette head 10 is loaded into the loading component 200, the end of the loading component 200 is inserted into the pipette head 10. The cavity 210 inside the loading component 200 is connected to the inner cavity of the pipette head 10. The pipetting mechanism uses a pump or other power components to provide the power for aspiration and dispensing, drawing the sample or reagent to be tested into the pipette head 10 or dispensing it from the pipette head 10. During the aspiration and dispensing process, the pipette head 10 is inserted into the container containing the sample or reagent along a first direction. After the pipette head 10 contacts the container, the loading component 200 slides relative to the loading support 100 along a second direction. The first elastic element 300 deforms as the loading component 200 slides and provides a force in the first direction to the loading component 200, ensuring stable contact between the pipette head 10 and the sample container, and making the aspiration and dispensing of the pipette head 10 smoother.

[0057] In one embodiment, the pipetting mechanism further includes a drive module 500 connected to the loading bracket 100. The drive module 500 is configured to at least drive the loading bracket 100 to move up and down, or at least drive the loading bracket 100 to translate, or at least drive the loading bracket 100 to rotate, so that the loading bracket 100 carries the loading component 200 closer to or further away from the pipette head 10 for loading the pipette head 10, or carries the loading component 200 and the pipette head 10 closer to or further away from the sample container for aspiration and dispensing operations, or carries the loading component 200 and the pipette head 10 between different working modules for pipetting operations. The drive module 500 is not limited to being a multi-degree-of-freedom robotic arm, a multi-axis module, etc.

[0058] Understandably, the sum of the elastic forces of the first elastic element 300 and the second elastic element 400 provides the actual loading force during the loading process. When the sum of the elastic forces of the first elastic element 300 and the second elastic element 400 is not less than the actual loading force required for loading the pipette head 10, the pipette head 10 is securely loaded onto the loading member 200. Based on the loading requirements of the pipette head 10, a preset range of the loading force required by the pipetting mechanism can be pre-set. When the sum of the elastic forces of the first elastic element 300 and the second elastic element 400 reaches the preset range of the loading force, the pipette head 10 can be securely loaded onto the loading member 200.

[0059] As one way to provide a loading force within a preset range for the pipetting mechanism, the drive module 500 is configured to drive the loading bracket 100 to move along a first direction or a second direction. During the loading process of the pipetting head 10, the drive module 500 continuously drives the loading bracket 100 to move along the first direction. The first elastic element 300 begins to deform after the loading element 200 contacts the pipetting head 10 and generates a first elastic force, which is directed towards the first direction. When the first elastic element 300 reaches a first preset deformation amount, the second elastic element 400 begins to deform and generates a second elastic force, which is directed towards the first direction. When the sum of the first elastic force and the second elastic force is within the preset range of the loading force, the drive module 500 stops driving the loading bracket 100 to move along the first direction. At this time, the pipetting head 10 is loaded. Since the actual loading force provided by the pipetting mechanism is within the preset range of the loading force, the loading of the pipetting head 10 is more stable and reliable.

[0060] As another way to provide a preset range of loading force for the pipetting mechanism, the drive module 500 is configured to drive the loading bracket 100 to move along a first direction or a second direction. When the drive module 500 drives the loading bracket 100 to move along the first direction, the loading component 200 follows the loading bracket 100 and moves along the first direction, approaching the pipetting head 10. During the loading process of the pipetting head 10, the drive module 500 continuously drives the loading bracket 100 to move along the first direction. The first elastic element 300 begins to deform after the loading component 200 contacts the pipetting head 10. When the first elastic element 300 reaches a first preset deformation amount, the second elastic element 400 begins to deform. When the first elastic element 300 reaches a second preset deformation amount, the pipetting head 10 is loaded, and the drive module 500 stops driving the loading bracket 100 to move along the first direction. The second preset deformation amount is greater than the first preset deformation amount. Alternatively, when the second elastic element 400 reaches a third preset deformation amount, the pipetting head 10 is loaded, and the drive module 500 stops driving the loading bracket 100 to move along the first direction.

[0061] According to the loading requirements of the pipette head 10, a second preset deformation amount of the first elastic element 300 or a third preset deformation amount of the second elastic element 400 can be preset. When the first elastic element 300 deforms to the second preset deformation amount or the second elastic element 400 deforms to the third preset deformation amount, the sum of the elastic force of the first elastic element 300 and the elastic force of the second elastic element 400 is within the preset range of the loading force, so that the loading force provided by the pipetting mechanism can meet the loading requirements of different types and different stages of use of the pipette head 10, and the loading of the pipette head 10 is more stable and reliable.

[0062] Understandably, the preset range of the loading force provided by the pipetting mechanism can be set to be greater than the loading force required for the pipetting head 10 to load. Therefore, when the drive module 500 stops driving the loading bracket 100 to move in the first direction, the actual loading force provided by the pipetting mechanism must be greater than the loading force required for the pipetting head 10 to complete the loading, so as to ensure that the pipetting head 10 is stably loaded on the loading component 200.

[0063] In some embodiments, reference is made to Figure 3 The pipetting mechanism includes a first sensing element 101 and a first triggering element 102. The first sensing element 101 can sense and be triggered by the first triggering element 102. The first sensing element 101 is connected to the drive module 500, and the first triggering element 102 is connected to the loading bracket 100. When the drive module 500 drives the loading bracket 100 to move along a first direction or a second direction, the first triggering element 102 moves synchronously with the loading bracket 100. During the loading process of the pipetting head 10, the drive module 500 continuously drives the loading bracket 100 to move along the first direction, and the loading member 200 moves relative to the loading bracket 100 along the second direction. When the loading bracket 100 moves to the point where the first elastic member 300 reaches a second preset deformation amount or the second elastic member 400 reaches a third preset deformation amount, the first sensing element 101 is triggered by the first triggering element 102. The drive module 500 stops driving the loading bracket 100 to move along the first direction according to the trigger signal of the first sensing element 101, thereby realizing the automatic loading of the pipetting head 10.

[0064] In one implementation of triggering the first sensing element 101, the first sensing element 101 is configured as a position sensor, such as a photoelectric position sensor, an electromagnetic position sensor, or a Hall effect position sensor. The first triggering element 102 is configured as a baffle. The first sensing element 101 can sense the position of the first triggering element 102. When the first triggering element 102 moves to the corresponding position with the loading bracket 100, the first sensing element 101 senses the first triggering element 102 and is triggered. In another implementation of triggering the first sensing element 101, the first sensing element 101 is configured as a displacement sensor, such as a linear grating sensor. The first sensing element 101 can detect the displacement of the first triggering element 102. When the first triggering element 102 moves a preset distance with the loading bracket 100, the first sensing element 101 is triggered by the first triggering element 102.

[0065] In some embodiments, reference is made to Figure 3The pipetting mechanism includes a second sensing element 103 and a second triggering element 104. The second sensing element 103 can sense and be triggered by the second triggering element 104. The second sensing element 103 is connected to the loading bracket 100, and the second triggering element 104 is connected to the loading component 200. During the loading process of the pipetting head 10, after the loading component 200 contacts the pipetting head 10, the loading component 200 continuously moves relative to the loading bracket 100 along the second direction, causing the first elastic element 300 and the second elastic element 400 to deform sequentially. When the first elastic element 300 reaches a second preset deformation amount or the second elastic element 400 reaches a third preset deformation amount, the second sensing element 103 is triggered by the second triggering element 104. The drive module 500 stops driving the loading bracket 100 to move according to the trigger signal of the second sensing element 103, thereby realizing the automatic loading of the pipetting head 10.

[0066] In one implementation of triggering the second sensing element 103, the second sensing element 103 is configured as a position sensor, such as a photoelectric position sensor, an electromagnetic position sensor, or a Hall effect position sensor. The second triggering element 104 is configured as a baffle. The second sensing element 103 can sense the position of the second triggering element 104. When the second triggering element 104 moves to the corresponding position along with the loading component 200, the second sensing element 103 senses the second triggering element 104 and is triggered. In another implementation of triggering the second sensing element 103, the second sensing element 103 is configured as a distance sensor. The second sensing element 103 can detect the distance between itself and the second triggering element 104. When the second sensing element 103 detects that the distance between itself and the second triggering element 104 is within a preset range, the second sensing element 103 is triggered.

[0067] In some embodiments, reference is made to Figure 3The pipetting mechanism includes a pressure detection element 105, which is connected to the loading bracket 100. The pressure detection element 105 is used to detect the pressure applied to the loading bracket 100 by the first elastic member 300 or the pressure applied to the loading bracket 100 by the second elastic member 400. Specifically, since both the first elastic element 300 and the second elastic element 400 are disposed between the loading bracket 100 and the loading member 200, the first elastic element 300 deforms as the loading member 200 moves relative to the loading bracket 100 in the second direction, generating a first elastic force. The first elastic force is applied to the loading bracket 100, forming pressure from the first elastic element 300 on the loading bracket 100. Similarly, after the first elastic element 300 reaches a first preset deformation amount, the second elastic element 400 deforms as the loading member 200 moves relative to the loading bracket 100 in the second direction, generating a second elastic force. The second elastic force is applied to the loading bracket 100, forming pressure from the second elastic element 400 on the loading bracket 100. The sum of the first elastic force and the second elastic force is the actual loading force. When the detection value of the pressure detection element 105 reaches the preset pressure range, the sum of the first elastic force and the second elastic force is within the preset range of the loading force. At this time, the drive module 500 stops driving the loading bracket 100 to move in the first direction, realizing the automatic loading of the pipette head 10.

[0068] Understandably, the drive module 500 can also be configured to stop driving the loading bracket 100 to move along the first direction and drive the loading bracket 100 to move along the second direction when the first sensing element 101 is triggered by the first triggering element 102, or the second sensing element 103 is triggered by the second triggering element 104, or the detection value of the pressure detection element 105 reaches a preset pressure range. This allows the pipette head 10, after being loaded onto the loading member 200, to be transferred to other positions along with the loading member 200. For example, the pipette head 10 is placed centrally in the pipette head holder before loading. After loading, the pipette head 10 moves along the second direction along with the loading member 200 and detaches from the pipette head holder.

[0069] Different types of pipette tips 10, or pipette tips 10 that need to be used multiple times, have different loading force requirements at different stages of use. For example, after multiple uses, the pipette tip 10 may deform uncontrollably, requiring a greater loading force in subsequent uses to improve the reliability of loading. Based on this, in some embodiments, the pipetting mechanism is provided with pre-tightening measures for the first elastic element 300 and / or the second elastic element 400. That is, before loading the pipette tip 10, the first elastic element 300 and / or the second elastic element 400 are pre-deformed. During the loading process, the first elastic element 300 and the second elastic element 400... The elastic element 400 further deforms based on the initial deformation amount, thereby increasing the first elastic force provided by the first elastic element 300 when it reaches the second preset deformation amount, and / or the second elastic force provided by the second elastic element 400 when it reaches the third preset deformation amount. This enhances the loading force provided by the pipetting mechanism during the loading of the pipetting head 10, without changing the travel of the loading support 100 during the loading process. By changing the preload of the first elastic element 300 and / or the second elastic element 400, the pipetting mechanism can meet the loading requirements of different types of pipetting heads 10 and pipetting heads 10 at different stages of use.

[0070] Specifically, refer to Figure 4 The pipetting mechanism includes a first pre-tightening seat 106 and a second pre-tightening seat 107. In some embodiments, the first pre-tightening seat 106 is connected to the loading member 200, and the second pre-tightening seat 107 is connected to the loading bracket 100. The two ends of the first elastic member 300 are respectively connected to the first pre-tightening seat 106 and the second pre-tightening seat 107. The first pre-tightening seat 106 and / or the second pre-tightening seat 107 can move along a first direction or a second direction to change the distance between the first pre-tightening seat 106 and the second pre-tightening seat 107, thereby adjusting the pre-tightening amount of the first elastic member 300.

[0071] The preload of the first elastic element 300 can be adjusted by pre-compressing or stretching the first elastic element 300. As one way to adjust the preload of the first elastic element 300, based on the free state of the first elastic element 300, by moving the first preload seat 106 and / or the second preload seat 107, the distance between the first preload seat 106 and the second preload seat 107 is shortened, which can compress the first elastic element 300. By changing the distance between the first preload seat 106 and the second preload seat 107, the pre-compression amount of the first elastic element 300 can be adjusted. During the loading process of the pipette head 10, the first elastic element 300 is further compressed as the loading component 200 slides relative to the loading bracket 100, and sequentially reaches the first preset deformation amount and the second preset deformation amount. As another way to adjust the preload of the first elastic element 300, in the free state of the first elastic element 300, by moving the first preload seat 106 and / or the second preload seat 107, the distance between the first preload seat 106 and the second preload seat 107 is increased, which can cause the first elastic element 300 to elongate. Increasing the distance between the first preload seat 106 and the second preload seat 107 can adjust the pre-elongation of the first elastic element 300. During the loading process of the pipette head 10, the first elastic element 300 further elongates as the loading component 200 slides relative to the loading bracket 100, and sequentially reaches the first preset deformation amount and the second preset deformation amount.

[0072] The pipetting mechanism also includes a third pre-tightening seat 108 and a fourth pre-tightening seat 109. In some embodiments, the third pre-tightening seat 108 is connected to the loading member 200, and the fourth pre-tightening seat 109 is connected to the loading bracket 100. The two ends of the second elastic member 400 are respectively connected to the third pre-tightening seat 108 and the fourth pre-tightening seat 109. The third pre-tightening seat 108 and / or the fourth pre-tightening seat 109 can move along a first direction or a second direction to change the distance between the third pre-tightening seat 108 and the fourth pre-tightening seat 109, thereby adjusting the pre-tightening amount of the second elastic member 400. Similarly, the preload adjustment of the second elastic element 400 can be achieved by pre-compressing or stretching the second elastic element 400. That is, by changing the distance between the third preload seat 108 and the fourth preload seat 109, the pre-compression amount of the second elastic element 400 can be adjusted. During the loading process of the pipette head 10, the second elastic element 400 is further compressed as the loading component 200 slides relative to the loading bracket 100, and reaches the third preset deformation amount. Alternatively, by changing the distance between the third preload seat 108 and the fourth preload seat 109, the pre-extension amount of the second elastic element 400 can be adjusted. During the loading process of the pipette head 10, the second elastic element 400 is further extended as the loading component 200 slides relative to the loading bracket 100, and reaches the third preset deformation amount.

[0073] Before loading the pipette head 10, the preload of either the first elastic element 300 or the second elastic element 400 can be adjusted according to the loading requirements of the pipette head 10, or both the preload of the first elastic element 300 and the second elastic element 400 can be adjusted; the preload or pre-extension of both the first elastic element 300 and the second elastic element 400 can be adjusted, or one of the first elastic element 300 and the second elastic element 400 can be adjusted for preload, and the other for pre-extension.

[0074] In some embodiments, such as Figure 4 As shown, the first elastic member 300 and the second elastic member 400 are arranged along the first direction. The first elastic member 300 is closer to the end of the loading member 200 used to load the pipette head 10 than the second elastic member 400. The first pre-tightening seat 106, the second pre-tightening seat 107, the third pre-tightening seat 108 and the fourth pre-tightening seat 109 are arranged sequentially along the first direction. The first pre-tightening seat 106 is closer to the end of the loading member 200 used to load the pipette head 10 than the second pre-tightening seat 107. Taking the compression of the first elastic element 300 and the second elastic element 400 during the loading process of the pipette head 10 as an example, when adjusting the preload of the first elastic element 300, the position of the first preload seat 106 connected to the loading component 200 is changed along the second direction, and / or the position of the second preload seat 107 connected to the loading bracket 100 is changed along the first direction, so that the first elastic element 300 is compressed; when adjusting the preload of the second elastic element 400, the position of the third preload seat 108 connected to the loading component 200 is changed along the second direction, and / or the position of the fourth preload seat 109 connected to the loading bracket 100 is changed along the first direction, so that the second elastic element 400 is compressed.

[0075] It should be noted that simultaneously changing the position of the first pretensioner 106 connected to the loading member 200 and the position of the second pretensioner 107 connected to the loading bracket 100 can not only change the distance between the first pretensioner 106 and the second pretensioner 107, but also adjust the distance between the first elastic member 300 and the second elastic member 400. This allows the first elastic member 300 to move closer to the second elastic member 400, thereby reducing the first preset deformation amount of the first elastic member 300, or to move the first elastic member 300 further away from the second elastic member 400, thereby increasing the first preset deformation amount of the first elastic member 300. Similarly, simultaneously changing the position of the third pretensioner 108 connected to the loading member 200 and the position of the fourth pretensioner 109 connected to the loading bracket 100 can also adjust the pretension amount of the second elastic member 400 and the first preset deformation amount of the first elastic member 300.

[0076] Reference Figure 2 and Figure 5In some embodiments, the pipetting mechanism includes an adjusting seat 110 connected to the loading bracket 100. A first pre-tightening seat 106 and a fourth pre-tightening seat 109 are located on opposite sides of the adjusting seat 110. The opposite ends of a first elastic member 300 are connected to the adjusting seat 110 and the first pre-tightening seat 106, respectively. The opposite ends of a second elastic member 400 are connected to the adjusting seat 110 and the fourth pre-tightening seat 109, respectively. That is, the second pre-tightening seat 107 and the third pre-tightening seat 108 form the same structure, which simplifies the structure of the pipetting mechanism. The adjusting seat 110 and the first pre-tightening seat 106 cooperate to adjust the pre-tightening amount of the first elastic member 300, and the adjusting seat 110 and the fourth pre-tightening seat 109 cooperate to adjust the pre-tightening amount of the second elastic member 400. At the same time, changing the position of the adjusting seat 110 and the first pre-tightening seat 106 or changing the position of the adjusting seat 110 and the fourth pre-tightening seat 109 can adjust the first preset deformation amount of the first elastic member 300.

[0077] The connection method between the first preload seat 106 and the loading component 200 is not limited to threaded connection, snap-fit, magnetic connection, etc., such as Figure 4 and Figure 5 As shown, in some embodiments, the first pretensioner 106 is sleeved on the outside of the loading member 200 and threadedly connected to the loading member 200. Tightening the first pretensioner 106 can change its installation position on the loading member 200, thereby achieving stepless adjustment of the pretension amount of the first elastic member 300. Understandably, the third pretensioner 108 can be connected to the loading member 200 using the same connection method.

[0078] The connection method between the second preload seat 107 and the loading bracket 100 is not limited to threaded connection, snap-fit, magnetic connection, etc., such as Figure 3 As shown, in some embodiments, the side of the second preload seat 107 is threadedly connected to the loading bracket 100. The loading bracket 100 is provided with a mounting groove 120 extending in a first direction. A threaded fastener passes through the mounting groove 120 and is connected to the second preload seat 107. The threaded fastener can move within the mounting groove 120 in either the first or second direction. By changing the position of the threaded fastener within the mounting groove 120, the position of the second preload seat 107 connected to the loading bracket 100 can be adjusted, thereby achieving stepless adjustment of the preload amount of the first elastic element 300. It is understood that the fourth preload seat 109 can be connected to the loading bracket 100 using the same connection method.

[0079] The connection methods between the first pretensioning seat 106 and the first elastic member 300, between the second pretensioning seat 107 and the first elastic member 300, between the third pretensioning seat 108 and the second elastic member 400, and between the fourth pretensioning seat 109 and the second elastic member 400 are not limited to abutment, welding, or bonding. In some embodiments, the third pretensioning seat 108 and the fourth pretensioning seat 109 abut against the second elastic member 400 through other components, such as... Figure 2 As shown, the loading member 200 passes through the first pre-tightening seat 106, the adjusting seat 110, and the fourth pre-tightening seat 109. The pipetting mechanism also includes a movable seat 111, which is sleeved on the outside of the loading member 200 and slidably connected to it. The end of the second elastic member 400 facing the loading member 200 for loading the pipetting head 10 is connected to the movable seat 111. The side of the movable seat 111 facing the loading member 200 for loading the pipetting head 10 abuts against the adjusting seat 110. The adjusting seat 110 is connected to the loading bracket 100. Changing the adjusting seat 110... The preload of the second elastic element 400 can be adjusted at the connection position on the loading bracket 100. The pipetting mechanism also includes a fixed seat 112. The loading element 200 is connected to the fourth preload seat 109 through the fixed seat 112. The movable seat 111 is closer to the end of the loading element 200 used for loading the pipetting head 10 than the fixed seat 112. The side of the fixed seat 112 facing away from the end of the loading element 200 used for loading the pipetting head 10 abuts against the fourth preload seat 109. By changing the connection position of the fourth preload seat 109 on the loading bracket 100, the preload of the second elastic element 400 can be adjusted.

[0080] The fixing seat 112 is provided to facilitate the fixed connection between the second elastic element 400 and the fourth pretension seat 109. It is understood that the fixing seat 112 and the fourth pretension seat 109 can be an integral structure, or they can be independent structures connected to each other. Alternatively, in other embodiments, the fixing seat 112 can be omitted, and the second elastic element 400 can be directly connected to the fourth pretension seat 109.

[0081] In some embodiments, before the loading member 200 contacts the pipette head 10, the first elastic member 300 has a first deformation amount L. 11 First deformation L 11 This refers to the preload of the first elastic element 300. After the pipette head 10 is loaded onto the loading member 200, the first elastic element 300 has a second deformation amount L. 12 The second deformation amount L 12 This refers to the deformation amount when the first elastic element 300 reaches the second preset deformation amount, where the original length of the first elastic element 300 is L. 10 Where 0.8≤L 11 / L 10 ≤1, 0.5≤L 12 / L 10 ≤0.8, L 11 / L 10 It can be set to values ​​such as 0.8, 0.85, 0.88, 0.9, 0.92, 0.95, 0.98, 1, etc., L 12 / L 10The values ​​are not limited to 0.5, 0.52, 0.55, 0.6, 0.65, 0.68, 0.7, 0.75, 0.8, etc.; by setting the preload of the first elastic element 300 and the second preset deformation amount within a preset range, on the one hand, the first elastic element 300, with a certain preload, further deforms during the loading of the pipette head 10, increasing the elastic force provided by the first elastic element 300 during loading; on the other hand, the first elastic element 300 can provide elastic force within the preset range when the second preset deformation amount is reached, so that the loading force provided by the pipetting mechanism can meet the loading requirements of the pipette head 10, and the loading of the pipette head 10 is more reliable.

[0082] In some embodiments, before the loading member 200 contacts the pipette head 10, the second elastic member 400 has a third deformation amount L. 21 The third deformation amount L 21 This refers to the preload of the first elastic element 300. After the pipette head 10 is loaded onto the loading member 200, the second elastic element 400 has a fourth deformation amount L. 22 The fourth deformation amount L 22 This refers to the deformation amount when the second elastic element 400 reaches the third preset deformation amount, and the original length of the second elastic element 400 is L. 20 Where 0.9≤L 21 / L 20 ≤1, 0.7≤L 22 / L 20 ≤0.9, L 21 / L 20 It is not limited to setting it to 0.9, 0.92, 0.93, 0.95, 0.97, 0.98, 1, etc., L 22 / L 20 The values ​​are not limited to 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 0.98, 1, etc. Similarly, by setting the preload of the second elastic element 400 and the third preset deformation amount within a preset range, on the one hand, the second elastic element 400, with a certain preload, further deforms during the loading of the pipette head 10, increasing the elastic force provided by the second elastic element 400 during loading; on the other hand, the second elastic element 400 can provide elastic force within the preset range when the third preset deformation amount is reached, so that the loading force provided by the pipetting mechanism can meet the loading requirements of the pipette head 10, achieving reliable loading of the pipette head 10.

[0083] It should be noted that the aforementioned deformation amount refers to the deformation length of the first elastic element 300 or the second elastic element 400 based on its original length. Specifically, the first deformation amount L 11 This refers to the first elastic element 300 before the loading element 200 contacts the pipette head 10, compared to its original length L. 10The deformation length, the second deformation amount L 12 This refers to the first elastic element 300 after the pipette head 10 is loaded onto the loading element 200, compared to its original length L. 10 The deformation length, or the length of the first elastic element 300 when it reaches the second preset deformation amount compared to the original length L. 10 The deformation length, the third deformation amount L 21 This refers to the second elastic element 400 before the loading element 200 contacts the pipette head 10, compared to its original length L. 20 The deformation length, the fourth deformation amount L 22 This refers to the second elastic element 400 after the pipette head 10 is loaded onto the loading element 200, compared to its original length L. 20 The deformation length, or the length of the second elastic element 400 when it reaches the third preset deformation amount compared to the original length L. 20 The deformation length.

[0084] In some embodiments, the original length L 10 This refers to the length of the first elastic element 300 in its natural state (unstressed state), and the first deformation amount L. 11 Second deformation amount L 12 This refers to the compressed length or elongated length of the first elastic element 400 after being subjected to force, compared to its natural state; the original length L 20 This refers to the length of the second elastic element 400 in its natural state (unstressed state), and the third deformation amount L. 21 Fourth deformation amount L 22 It refers to the compression length or elongation length of the second elastic element 400 after being subjected to force, compared to its natural state.

[0085] In some embodiments, the stiffness of the first elastic element 300 and / or the second elastic element 400 can be changed to enable the first elastic element 300 and the second elastic element 400 to provide a larger elastic force based on a preset deformation amount, so as to meet the loading force requirements of different types or different stages of use of the pipette head 10. Specifically, the stiffness of the first elastic element 300 is k1, 0.56 N / mm ≤ k1 ≤ 0.84 N / mm, and k1 is not limited to being set to 0.56, 0.6, 0.65, 0.68, 0.7, 0.75, 0.8, 0.84, etc. When having the same deformation amount, the first elastic element 300 with a larger stiffness can provide a larger first elastic force. By setting the stiffness k1 of the first elastic element 300 within the corresponding range, the first elastic element 300 can provide an elastic force within the preset range when the second preset deformation amount is reached, so that the loading force provided by the pipetting mechanism can meet the loading requirements of the pipette head 10, and the loading of the pipette head 10 is more reliable.

[0086] The stiffness of the second elastic element 400 is k2, where 4.64 N / mm ≤ k2 ≤ 6.96 N / mm. k2 is not limited to values ​​such as 4.64, 4.68, 4.5, 4.55, 4.6, 4.7, 4.8, 5, 5.2, 5.3, 5.5, 5.6, 5.8, 6, 6.2, 6.5, 6.6, 6.7, 6.8, 6.9, 6.95, or 6.96. With the same amount of deformation, the second elastic element 400 with greater stiffness can provide a larger second elastic force. By setting the stiffness k2 of the second elastic element 400 within the corresponding range, the second elastic element 400 can provide an elastic force within the preset range when the third preset deformation is reached. This ensures that the loading force provided by the pipetting mechanism can meet the loading requirements of the pipetting head 10, making the loading of the pipetting head 10 more reliable.

[0087] The first elastic element 300 and the second elastic element 400 can provide elastic force during the loading process of the pipette head 10 by means of elongation or compression, thereby achieving flexible loading of the pipette head 10. In some embodiments, refer to Figure 5 The first elastic member 300 has a first end 310 and a second end 320 disposed along a first direction. The second end 320 is closer to the end of the loading member 200 used for loading the pipette tip 10 than the first end 310. The first end 310 is connected to the loading bracket 100, and the second end 320 is connected to the loading member 200. For example, the first end 310 is connected to the loading bracket 100 via an adjusting member 110, and the second end 320 is connected to the loading member 200 via a first preload seat 106. During the loading of the pipette tip 10, after the loading member 200 contacts the pipette tip 10, the loading member 200 moves relative to the loading bracket 100 along the second direction. The second end 320 moves synchronously with the loading member 200 and gradually approaches the first end 310. The first elastic member 300 is compressed as the loading member 200 slides. In this case, the first elastic member 300 provides elastic force in a compressed manner. Alternatively, in some other embodiments, refer to Figure 4 The first end 310 of the first elastic element 300 is connected to the loading element 200, and the second end 320 is connected to the loading bracket 100. For example, the first end 310 is fixedly connected to the loading element 200 through the second preload seat 107, and the second end 320 is fixedly connected to the loading bracket 100 through the first preload seat 106. During the loading process of the pipette head 10, after the loading element 200 contacts the pipette head 10, the loading element 200 moves relative to the loading bracket 100 in the second direction. The first end 310 moves synchronously with the loading element 200 and gradually moves away from the second end 320. The first elastic element 300 is stretched as the loading element 200 slides. In this case, the first elastic element 300 provides elastic force in an elongated manner.

[0088] Similarly, in some embodiments, reference is made to Figure 5The second elastic member 400 has a third end 410 and a fourth end 420 disposed along a first direction. The fourth end 420 is closer to the end of the loading member 200 used for loading the pipette head 10 than the third end 410. The third end 410 is connected to the loading bracket 100, and the fourth end 420 is connected to the loading member 200. For example, the third end 410 is fixedly connected to the loading bracket 100 via a fourth pre-tightening seat 109, and the fourth end 420 is connected to the loading member 200 via a movable seat 111. During the loading of the pipette head 10, after the first elastic member 300 reaches a first preset deformation amount, the loading member 200 continues to move relative to the loading bracket 100 along the second direction. The fourth end 420 moves synchronously with the loading member 200 and gradually approaches the third end 410. The second elastic member 400 is compressed as the loading member 200 slides. In this case, the second elastic member 400 provides elastic force in a compressed manner. Alternatively, in some other embodiments, refer to Figure 4 The third end 410 of the second elastic member 400 is connected to the loading member 200, and the fourth end 420 is connected to the loading bracket 100. For example, the third end 410 is fixedly connected to the loading member 200 through the fourth pre-tightening seat 109, and the fourth end 420 is fixedly connected to the loading bracket 100 through the third pre-tightening seat 108. After the first elastic member 300 reaches the first preset deformation amount, the loading member 200 continues to move relative to the loading bracket 100 in the second direction. The third end 410 moves synchronously with the loading member 200 and gradually moves away from the fourth end 420. The second elastic member 400 is stretched along with the sliding of the loading member 200. In this case, the second elastic member 400 provides elastic force in an elongated manner.

[0089] It should be noted that both the first elastic element 300 and the second elastic element 400 can provide elastic force by compression, or both can provide elastic force by stretching, or one of the first elastic element 300 and the second elastic element 400 can provide elastic force by compression, and the other can provide elastic force by stretching. Figure 2 and Figure 5As shown, taking the example where both the first elastic element 300 and the second elastic element 400 provide elastic force through compression, the first end 310 of the first elastic element 300 is connected to the loading bracket 100, and the second end 320 is connected to the loading component 200. The third end 410 of the second elastic element 400 is connected to the loading bracket 100, and the fourth end 420 is connected to the loading component 200. During the loading process of the pipette head 10, after the loading component 200 contacts the pipette head 10, the loading component 200 slides relative to the loading bracket 100 in the second direction. The second end 320 of the first elastic element 300 gradually approaches the first end 310, and the first elastic element 300 is compressed. When the first elastic element 300 is compressed to the first preset deformation amount, the fourth end 420 of the second elastic element 400 gradually approaches the third end 410, and the second elastic element 400 is compressed until the first elastic element 300 reaches the second preset deformation amount, or the second elastic element 400 reaches the third preset deformation amount.

[0090] In some embodiments, reference is made to Figures 2 to 5 The arrangement direction of the first elastic element 300 and the second elastic element 400 is parallel to the sliding direction of the loading member 200. That is, the first elastic element 300 and the second elastic element 400 are arranged along the first direction or the second direction. This arrangement can make full use of the space along the sliding direction of the loading member 200, and the pipetting mechanism is more compactly arranged in the radial direction of the loading member 200. For example, the first end 310 of the first elastic element 300 is closer to the end of the loading member 200 used to load the pipetting head 10 than the fourth end 420 of the second elastic element 400. Both the first elastic element 300 and the second elastic element 400 can be deformed by stretching or compressing. After the loading member 200 contacts the pipetting head 10, as the loading member 200 slides relative to the loading support 100 along the second direction, the first elastic element 300 and the second elastic element 400 deform sequentially.

[0091] In some embodiments, reference is made to Figure 6 The arrangement direction of the first elastic element 300 and the second elastic element 400 intersects the sliding direction of the loading member 200, that is, the first elastic element 300 and the second elastic element 400 are arranged radially along the loading member 200, so as to make full use of the space along the radial direction of the loading member 200 and reduce the length of the pipetting mechanism in the sliding direction of the loading member 200, such as... Figure 6In the illustrated embodiment, the arrangement direction of the first elastic member 300 and the second elastic member 400 is perpendicular to the sliding direction of the loading member 200. Specifically, the second end 320 of the first elastic member 300 is closer to the end of the loading member 200 used for loading the pipette head 10 than the fourth end 420 of the second elastic member 400. The first end 310 of the first elastic member 300 and the third end 410 of the second elastic member 400 are both connected to the loading bracket 100. A pushing part 220 is provided on the periphery of the loading member 200. The second end 320 of the first elastic member 300 is connected to the pushing part 220. There is a gap between the fourth end 420 of the second elastic member 400 and the pushing part 220. 0. The pusher 220 is connected to the loading member 200. During the loading process of the pipette head 10, after the loading member 200 contacts the pipette head 10, the loading member 200 slides relative to the loading bracket 100 in the second direction. The pusher 220 pushes the second end 320 gradually closer to the first end 310, so that the first elastic member 300 is compressed. When the first elastic member 300 is compressed to reach the first preset deformation amount, the pusher 220 contacts the fourth end 420 of the second elastic member 400 and pushes the fourth end 420 gradually closer to the third end 410, so that the second elastic member 400 is compressed.

[0092] It should be noted that in this embodiment, multiple second elastic elements 400 can be provided, and multiple second elastic elements 400 are arranged around the periphery of the first elastic element 300. The distance between the fourth end 420 of the multiple second elastic elements 400 and the pushing part 220 is the same, so that the multiple second elastic elements 400 are compressed by the pushing part 220 at the same time and provide a second elastic force together; or, the distance between the fourth end 420 of different second elastic elements 400 and the pushing part 220 is different. During the sliding of the loading member 200 relative to the loading bracket 100 in the second direction, the pushing part 220 contacts different second elastic elements 400 in sequence, so that the different second elastic elements 400 begin to deform one by one, thereby providing a multi-level buffering effect, which helps to avoid excessive deformation of the pipette head 10.

[0093] In some embodiments, reference is made to Figure 7The second elastic member 400 is sleeved outside the first elastic member 300 to fully utilize the space along the radial direction and sliding direction of the loading member 200, and to reduce the length of the pipetting mechanism in both the sliding and radial directions of the loading member 200. Specifically, the second end 320 of the first elastic member 300 is closer to the end of the loading member 200 used for loading the pipetting head 10 than the fourth end 420 of the second elastic member 400. The first end 310 of the first elastic member 300 and the third end 410 of the second elastic member 400 are both connected to the loading bracket 100. A pushing part 220 is provided on the periphery of the loading member 200. The second end 320 of the first elastic member 300 is connected to the pushing part 220, and the fourth end 420 of the second elastic member 400 is connected to the pushing part 220. There is a gap between 0, and the fourth end 420 of the second elastic member 400 is suspended; during the loading process of the pipette head 10, after the loading member 200 contacts the pipette head 10, the loading member 200 slides relative to the loading bracket 100 in the second direction, and the loading member 200 drives the pushing part 220 to slide in the second direction. The pushing part 220 pushes the second end 320 to gradually approach the first end 310, so that the first elastic member 300 is compressed. When the first elastic member 300 is compressed to reach the first preset deformation amount, the pushing part 220 contacts the fourth end 420 of the second elastic member 400 and pushes the fourth end 420 to gradually approach the third end 410, so that the second elastic member 400 is compressed.

[0094] As one way to trigger the deformation of the second elastic element 400, refer to Figure 2 and Figure 5The pipetting mechanism includes a movable seat 111, a loading member 200 having a sliding part 230 and a supporting part 240 disposed around the sliding part 230, the sliding part 230 having a free end 231 for loading a pipetting head 10, the movable seat 111 being slidably connected to the sliding part 230, the sliding part 230 being slidably connected to the loading bracket 100, the supporting part 240 sliding synchronously with the sliding part 230 relative to the loading bracket 100, one end of the second elastic member 400 facing away from the free end 231 of the sliding part 230 (i.e., the third end 410) being connected to the loading bracket 100, and one end of the second elastic member 400 facing the free end 231 of the sliding part 230 (i.e., the fourth end 420) being connected to the movable seat 111. For example, the fourth pre-tightening seat 109 is connected to the loading bracket 100, the third end 410 of the second elastic member 400 is connected to the fourth pre-tightening seat 109, and the fourth end 420 of the second elastic member 400 is connected to the movable seat 111. During the loading process of the pipette head 10, when the first elastic member 300 reaches the first preset deformation amount, the supporting part 240 continues to slide relative to the loading bracket 100 in the second direction following the sliding part 230, and the supporting part 240 abuts against the movable seat 111. The supporting part 240 pushes the movable seat 111 to gradually approach the end of the second elastic member 400 connected to the loading bracket 100, so that the second elastic member 400 is compressed, realizing the deformation of the second elastic member 400. By using the movable seat 111 to connect with the second elastic member 400 and push the second elastic member 400 to deform, the structure of the loading member 200 can be simplified, and the positional matching of the movable seat 111 and the second elastic member 400 can be facilitated.

[0095] As another way to trigger the deformation of the second elastic element 400, refer to Figure 8One end (i.e., the third end 410) of the free end 231 of the second elastic member 400 facing away from the sliding part 230 is connected to the loading bracket 100. The other end (i.e., the fourth end 420) of the second elastic member 400 facing the free end 231 of the sliding part 230 is suspended. For example, the fourth pre-tightening seat 109 is connected to the loading bracket 100, and the third end 410 of the second elastic member 400 is connected to the fourth pre-tightening seat 109. During the loading process of the pipette head 10, when the first elastic member 300 reaches the first preset deformation amount, the supporting part 240 continues to slide relative to the loading bracket 100 in the second direction along with the sliding part 230, and the supporting part 240 abuts against the suspended end of the second elastic member 400. The supporting part 240 directly pushes the suspended end of the second elastic member 400 to gradually approach the end of the second elastic member 400 connected to the loading bracket 100, so that the second elastic member 400 is compressed, thereby realizing the deformation of the second elastic member 400. By using the supporting portion 240 to deform the suspended end of the second elastic member 400, the structure and assembly process of the pipetting mechanism can be simplified. In another embodiment, a movable seat 111 is slidably fitted around the outer periphery of the sliding portion 230. When the supporting portion 240 slides relative to the loading bracket 100 in the second direction, it abuts against the movable seat 111, indirectly compressing the second elastic member 400. The movable seat 111 ensures reliable contact between the movable seat 111 and the second elastic member 400, thereby reliably compressing the second elastic member 400 during loading to provide a stable and reliable cushioning effect.

[0096] It should be noted that the above-mentioned deformation mode of the second elastic element 400 is applicable to the arrangement mode where the arrangement direction of the first elastic element 300 and the second elastic element 400 is parallel to the sliding direction of the loading element 200, the arrangement direction of the first elastic element 300 and the second elastic element 400 intersects with the sliding direction of the loading element 200, and the arrangement mode where the second elastic element 400 is sleeved on the outside of the first elastic element 300.

[0097] In some embodiments, the pushing part 220 and the supporting part 240 are sleeved on the outside of the sliding part 230; in other embodiments, the pushing part 220 and the supporting part 240 are configured as protrusions on the periphery of the sliding part 230, so that the pushing part 220 and the sliding part 230 form an integral connection structure, and the supporting part 240 and the sliding part 230 form an integral connection structure. For example, when the sliding part 230 is a shaft, the pushing part 220 and the supporting part 240 are configured as shoulders of the shaft.

[0098] In some embodiments, reference is made to Figure 1The pipetting mechanism includes a pusher seat 113. One end of the first elastic member 300 facing the loading member 200 for loading the pipetting head 10 is connected to the pusher seat 113, and the other end of the first elastic member 300 facing away from the loading member 200 for loading the pipetting head 10 is connected to the loading bracket 100. The pusher seat 113 is movably connected to a sliding part 230. Changing the connection position of the pusher seat 113 on the sliding part 230 adjusts the preload of the first elastic member 300. After the loading member 200 contacts the pipetting head 10, the pusher seat 113 slides relative to the loading bracket 100 in a second direction along the sliding part 230, causing the pusher seat 113 to deform the first elastic member 300. The pusher seat 113 has the functions of adjusting the preload of the first elastic member 300 and causing deformation of the first elastic member 300 during the loading of the pipetting head 10. That is, the pusher seat 113 and the first preload seat 106 are set as the same structure, effectively simplifying the structure of the pipetting mechanism.

[0099] Figure 1 In the illustrated embodiment, the push seat 113 and the first pre-tightening seat 106 are configured with the same structure, and the second pre-tightening seat 107 and the third pre-tightening seat 108 form the same structure, namely the adjusting seat 110. This simplifies the structure of the pipetting mechanism. In this embodiment, the push seat 113 or the first pre-tightening seat 106 is connected to the loading member 200, and the adjusting seat 110 and the fourth pre-tightening seat 109 are connected to the loading bracket 100. When the loading member 200 moves relative to the loading bracket 100 in the second direction, it drives the first pre-tightening seat 106 to move and compress the first elastic member 300. When the loading member 200 moves to the abutting portion 240 of the loading member 200 abutting the second elastic member 400, the second elastic member 400 is compressed as the loading member 200 continues to move in the second direction. Therefore, during the loading process of the pipette head 10, the elastic force generated by the sequential compression of the first elastic element 300 and the second elastic element 400 provides a flexible loading force, thereby protecting the loading component 200 and the pipette head 10 and preventing excessive deformation of the pipette head 10 from affecting the sealing of the loading.

[0100] In other embodiments, the push seat 113 and the first pre-tightening seat 106 are independent of each other and can be configured with different structures. The push seat 113 is connected to the first pre-tightening seat 106, and the second end 320 of the first elastic member 300 is connected to the push seat 113 through the first pre-tightening seat 106, thereby making it easier to adjust the pre-tightening amount of the first elastic member 300. In addition, the second pre-tightening seat 107 and the third pre-tightening seat 108 are independent of each other and configured with different structures. In this case, the second pre-tightening seat 107 is connected to the loading bracket 100, and the third pre-tightening seat 108 is connected to the fourth end 420 of the second elastic member 400, and the third pre-tightening seat 108 can slide along the loading member 200. When the loading member 200 moves relative to the loading bracket 100 in the second direction until the supporting part 240 of the loading member 200 abuts against the third pretension seat 108, the loading member 200 continues to move relative to the loading bracket 100 in the second direction and pushes the third pretension seat 108 to move, and the third pretension seat 108 compresses the second elastic member 400.

[0101] Additionally, refer to Figure 1 and Figure 2 The loading bracket 100 has a guide channel 130, through which the loading member 200 slidably passes. The guide channel 130 guides the sliding of the loading member 200 relative to the loading bracket 100, allowing the loading member 200 to slide relative to the loading bracket 100 along a first direction or a second direction. The cross-sectional shape of the portion of the guide channel 130 and the portion of the loading member 200 slidably connected within the guide channel 130 is the same. In some embodiments, to prevent rotation of the loading member 200 during sliding relative to the loading bracket 100, the cross-sections of the guide channel 130 and the loading member 200 are set as polygons. In some embodiments, the loading member 200 includes a sliding portion 230, and the push seat 113 or the first pre-tightening seat 106 is provided with a first guide portion 250. The first guide portion 250 is disposed on the periphery of the sliding portion 230. The loading bracket 100 includes a second guide portion 140, which is disposed on the side of the guide channel 130. Part 250 and second guide part 140 are slidably connected along a first direction or a second direction. One of the first guide part 250 and the second guide part 140 is configured as a column, and the other is configured as a slot structure. When the loading member 200 slides relative to the loading bracket 100 and drives the push seat 113 or the first pre-tightening seat 106 to slide, the sliding part 230 slides along the guide channel 130, and the second guide part 140 slides along the first guide part 250. Since the loading member 200 is fixedly connected to the push seat 113 or the first pre-tightening seat 106, the cooperation between the first guide part 250 and the second guide part 140 plays a role in preventing the loading member 200 from rotating.

[0102] In some embodiments, the first guide portion 250 and the first trigger element 102 are respectively disposed on different sides of the push seat 113 to make full use of the circumferential space of the loading member 200.

[0103] Taking the first elastic element 300 and the second elastic element 400 as examples, where the first elastic element 300 and the second elastic element 400 are deformed by compression, and their arrangement direction is parallel to the sliding direction of the loading member 200, for instance... Figure 1 and Figure 2 As shown, the first elastic member 300 and the second elastic member 400 are both sleeved on the outside of the sliding part 230. The first pre-tightening seat 106 is fixedly connected to the loading member 200. The sliding part 230 slidably passes through the adjusting seat 110 and the fourth pre-tightening seat 109. The first elastic member 300 is closer to the free end 231 of the loading member 200 than the second elastic member 400. The second end 320 of the first elastic member 300 is connected to the first pre-tightening seat 106, and the first end 310 is connected to the adjusting seat 110. The fourth end 420 of the second elastic member 400 is connected to the movable seat 111, and the third end 410 is connected to the fixed seat 112. The fixed seat 112 abuts against the fourth pre-tightening seat 109, and the movable seat 111 abuts against the adjusting seat 110. During the loading process of the pipette head 10, the drive module 500 drives the loading bracket 100 to move along the first direction. The free end 231 of the loading component 200 gradually approaches the pipette head 10. After the free end 231 of the loading component 200 contacts the pipette head 10, the loading component 200 slides relative to the loading bracket 100 along the second direction. The first pre-tightening seat 106 pushes the first elastic member 300 along the second direction, and the first elastic member 300 is compressed. When the first elastic member 300 is compressed to reach the first preset deformation amount, the abutment part 240 abuts against the movable seat 111 and pushes the movable seat 111 along the second direction, so that the second elastic member 400 is compressed until the first elastic member 300 reaches the second preset deformation amount or the second elastic member 400 reaches the third preset deformation amount, thus completing the loading of the pipette head 10.

[0104] To prevent the pipetting head 10 from failing during pipetting due to unknown operating conditions such as being subjected to forces in a second direction, or accidental collisions with other components, some embodiments refer to... Figure 3 The pipetting mechanism also includes a third sensing element 114 and a third triggering element 115. The third sensing element 114 is connected to the loading bracket 100, and the third triggering element 115 is connected to the loading member 200 and can move synchronously with the loading member 200. If the pipetting head 10 is subjected to an unknown force in the second direction during the pipetting process, the pipetting head 10 drives the loading member 200 to slide relative to the loading bracket 100 in the second direction. The first elastic member 300 deforms along with the sliding of the loading member 200 relative to the loading bracket 100 in the second direction. When the first elastic member 300 reaches a fourth preset deformation amount, the third sensing element 114 is triggered by the third triggering element 115 to issue an alarm signal. The pipetting mechanism outputs abnormal alarm information to facilitate fault diagnosis and prevent the fault from worsening. The alarm information output by the pipetting mechanism is not limited to displaying the fault location, displaying the fault type, or playing a prompt sound.

[0105] It should be noted that during the aspiration and dissipation process, the pipette tip 10 is inserted into the container along the first direction. If the pipette tip 10 is inserted too deeply, it may cause the aspiration and dissipation to be unsuccessful or damage to the transfer component 200 or the pipette tip 10. After the pipette tip 10 is inserted into the container along the first direction, if it is inserted too deeply and comes into contact with the bottom of the container, it will be subjected to a force from the container in the second direction. The loading component 200 will slide relative to the loading support 100 in the second direction, and the first elastic element 300 will deform to provide cushioning, which can better protect the loading component 200 and the pipette tip 10. Furthermore, since the pipetting mechanism is equipped with a third sensing element 114 and a third triggering element 115, when the pipette tip 10 is inserted into the sample container to a preset depth, the first elastic element 300 reaches a fourth preset deformation amount. The third sensing element 114 is triggered by the third triggering element 115 to issue an alarm signal, which prevents the pipette tip 10 from being inserted too deeply into the container and damaging the loading component 200 and the pipette tip 10, and makes the aspiration and dissipation process smoother.

[0106] Among them, the fourth preset deformation amount is less than the first preset deformation amount, that is, the deformation amount of the first elastic element 300 required to trigger the third sensing element 114 is less than the deformation amount of the first elastic element 300 required to cause the second elastic element 400 to start deforming when the pipetting head 10 is loaded, and the abnormal alarm of the pipetting mechanism during the pipetting process is more sensitive.

[0107] Since the first preset deformation amount reached by the first elastic element 300 during the loading of the pipette head 10 is greater than the fourth preset deformation amount reached by the first elastic element 300 after the pipette head 10 is loaded, the first elastic element 300 continues to deform to reach the first preset deformation amount after reaching the fourth preset deformation. In order to prevent the third triggering element 115 from triggering the third sensing element 114 and causing a false alarm during the loading of the pipette head 10 due to the first elastic element 300 reaching the fourth preset deformation amount, the pipetting mechanism is also equipped with a false alarm prevention measure to make the working conditions of the pipetting mechanism loading the pipette head 10, the pipetting working conditions after the pipette head 10 is loaded, and the aspiration and dispensing working conditions after the pipette head 10 is loaded independent of each other and do not affect each other.

[0108] As a method to prevent false alarms, the third sensing element 114 is in a signal shielding state during the loading process of the pipette head 10 onto the loading member 200. During this process, even if the first elastic member 300 reaches a fourth preset deformation amount, the third sensing element 114 will not trigger an alarm signal. After the pipette head 10 is loaded onto the loading member 200, the third sensing element 114 is in a signal acquisition state. When the first elastic member 300 reaches the fourth preset deformation amount, the third sensing element 114 is triggered by the third triggering element 115. The third sensing element 114 can trigger an alarm signal due to a malfunction caused by unknown operating conditions during the pipetting and aspiration / discharging process of the pipetting mechanism. The signal shielding method of the third sensing element 114 is not limited to adding a filter to the signal transmission line of the third sensing element 114. The filter attenuates or blocks the signal of the third sensing element 114, cutting off the outward signal transmission of the third sensing element 114.

[0109] As another way to prevent false alarms, the third sensing element 114 is set to be in a stopped state during the loading process of the pipette head 10 onto the loading member 200. During this process, even if the first elastic member 300 reaches a fourth preset deformation amount, the third sensing element 114 will not trigger an alarm signal. The third sensing element 114 is in a working state after the pipette head 10 is loaded onto the loading member 200. When the first elastic member 300 reaches the fourth preset deformation amount, the third sensing element 114 is triggered by the third triggering element 115 to trigger an alarm signal. The way to switch the working state of the third sensing element 114 is not limited to adding a circuit switch to the wire connected to the third sensing element 114. During the loading process of the pipette head 10, the circuit switch is turned off, and the third sensing element 114 stops working. After the pipette head 10 is loaded, the circuit switch is turned on, and the third sensing element 114 starts working.

[0110] In one implementation of triggering the third sensing element 114, the third sensing element 114 is configured as a position sensor, such as a photoelectric position sensor, an electromagnetic position sensor, or a Hall effect position sensor. The third triggering element 115 is configured as a baffle. The third sensing element 114 can sense the position of the third triggering element 115. When the third triggering element 115 moves to the corresponding position with the loading component 200, the third sensing element 114 senses the third triggering element 115 and is triggered. In another implementation of triggering the third sensing element 114, the third sensing element 114 is configured as a distance sensor. The third sensing element 114 can detect the distance between itself and the third triggering element 115. When the third sensing element 114 detects that the distance between itself and the third triggering element 115 is within a preset range, the third sensing element 114 is triggered.

[0111] This invention also provides an analyzer, which is not limited to being configured as a molecular diagnostic analyzer, an immunoassay analyzer, etc. In some embodiments, the analyzer includes a nucleic acid extraction module, an amplification module, a detection module, and the aforementioned pipetting mechanism. The nucleic acid extraction module is used to extract nucleic acids from a sample to obtain a nucleic acid extract, such as extracting nucleic acids from a biological sample using a magnetic bead method. The pipetting mechanism is at least used to transfer the nucleic acid extract to an amplification container. For example, the pipetting mechanism draws the nucleic acid extract through a pipette tip 10, then transfers the pipette tip 10 to the amplification container and discharges the nucleic acid extract into the amplification container. The amplification module is used to amplify the nucleic acid extract loaded in the amplification container to significantly increase the amount of nucleic acid in a short time and obtain a test solution, such as using polymerase chain reaction for nucleic acid amplification. The detection module is used to detect the test solution.

[0112] In some embodiments, the pipetting mechanism can also be used to transfer nucleic acid extraction reagents to a nucleic acid extraction container and to transfer biological samples from a sample container to a nucleic acid extraction container.

[0113] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof can be combined with each other unless otherwise specified.

Claims

1. A pipetting mechanism, characterized in that, include: Loading bracket; A loading member, slidably connected to the loading bracket and used for loading a pipette tip, is configured to: follow the loading bracket in a first direction to move closer to the pipette tip, and slide relative to the loading bracket in a second direction after contacting the pipette tip, wherein the first direction is opposite to the second direction; A first elastic element is disposed between the loading member and the loading bracket, and is capable of deforming along the second direction after the loading member contacts the pipette head and slides relative to the loading bracket, so as to provide a force to the loading member in the first direction; The second elastic element is disposed between the loading member and the loading bracket. The second elastic element can deform as the loading member continues to slide relative to the loading bracket in the second direction after the first elastic element reaches a first preset deformation amount, and provide a force to the loading member in the first direction.

2. The pipetting mechanism according to claim 1, characterized in that, The pipetting mechanism further includes a drive module, which is connected to the loading bracket and is used to drive the loading bracket to move along the first direction or the second direction. The drive module is configured to stop driving the loading bracket to move along the first direction when the first elastic element reaches a second preset deformation amount or the second elastic element reaches a third preset deformation amount, wherein the second preset deformation amount is greater than the first preset deformation amount.

3. The pipetting mechanism according to claim 2, characterized in that, The pipetting mechanism further includes a first sensing element and a first triggering element. The first sensing element is connected to the drive module, and the first triggering element is connected to the loading bracket. The first triggering element moves synchronously with the loading bracket. The drive module is configured such that the first sensing element is triggered by the first triggering element when the first elastic element reaches the second preset deformation amount or the second elastic element reaches the third preset deformation amount. The drive module stops driving the loading bracket to move according to the triggering signal of the first sensing element. Alternatively, the pipetting mechanism further includes a second sensing element and a second triggering element. The second sensing element is connected to the loading bracket, and the second triggering element is connected to the loading member. The second triggering element moves synchronously with the loading member. The drive module is configured such that the second sensing element is triggered by the second triggering element when the first elastic member reaches the second preset deformation amount or the second elastic member reaches the third preset deformation amount. The drive module stops driving the loading bracket to move according to the triggering signal of the second sensing element. Alternatively, the pipetting mechanism may further include a pressure detection element connected to the loading bracket. The pressure detection element is used to detect the pressure applied to the loading bracket by the first elastic element or the pressure applied to the loading bracket by the second elastic element. The drive module is configured to stop driving the loading bracket to move when the detection value of the pressure detection element reaches a preset pressure range.

4. The pipetting mechanism according to any one of claims 1 to 3, characterized in that, The pipetting mechanism includes a movable seat, the loading member has a sliding portion and a supporting portion disposed around the sliding portion, the sliding portion has a free end for loading the pipetting tip, the movable seat is slidably connected to the sliding portion, one end of the free end of the second elastic member facing away from the sliding portion is connected to the loading bracket, and one end of the free end of the second elastic member facing the sliding portion is connected to the movable seat, the loading member is configured such that: after the first elastic member reaches the first preset deformation amount, the supporting portion abuts against the movable seat and pushes the second elastic member to deform; Alternatively, the loading member has a sliding portion and a supporting portion disposed around the sliding portion. The sliding portion is slidably connected to the loading bracket, and the sliding portion has a free end for loading the pipette tip. One end of the free end of the second elastic member facing away from the sliding portion is connected to the loading bracket, and one end of the second elastic member facing the free end of the sliding portion is suspended. The loading member is configured such that after the first elastic member reaches the first preset deformation amount, the supporting portion abuts against the suspended end of the second elastic member and pushes the second elastic member to deform.

5. The pipetting mechanism according to any one of claims 1 to 3, characterized in that, The pipetting mechanism includes a first pre-tightening seat and a second pre-tightening seat. The first pre-tightening seat is connected to the loading component, and the second pre-tightening seat is connected to the loading bracket. The two ends of the first elastic element are respectively connected to the first pre-tightening seat and the second pre-tightening seat. The first pre-tightening seat and / or the second pre-tightening seat can move along the first direction or the second direction to adjust the pre-tightening amount of the first elastic element. And / or, the pipetting mechanism includes a third pre-tightening seat and a fourth pre-tightening seat, the third pre-tightening seat being connected to the loading member, the fourth pre-tightening seat being connected to the loading bracket, the two ends of the second elastic member being respectively connected to the third pre-tightening seat and the fourth pre-tightening seat, the third pre-tightening seat and / or the fourth pre-tightening seat being movable along the first direction or the second direction to adjust the pre-tightening amount of the second elastic member.

6. The pipetting mechanism according to any one of claims 1 to 3, characterized in that, Before the loading component contacts the pipette head, the first elastic element has a first deformation amount L. 11 After the pipette head is loaded onto the loading member, the first elastic member has a second deformation amount L. 12 The original length of the first elastic element is L. 10 Where 0.8≤L 11 / L 10 ≤1, 0.5≤L 12 / L 10 ≤0.8; Before the loading component contacts the pipette head, the second elastic element has a third deformation amount L. 21 After the pipette head is loaded onto the loading member, the second elastic member has a fourth deformation amount L. 22 The original length of the second elastic element is L. 20 Where 0.9≤L 21 / L 20 ≤1, 0.7≤L 22 / L 20 ≤0.9; And / or, the stiffness of the first elastic element is k1, the stiffness of the second elastic element is k2, 0.56N / mm≤k1≤0.84N / mm, 4.64N / mm≤k2≤6.96N / mm.

7. The pipetting mechanism according to claim 1, characterized in that, The first elastic member has a first end and a second end disposed along the first direction, the second end being closer than the first end to the end of the loading member used for loading the pipette tip; wherein, the first end is connected to the loading bracket, the second end is connected to the loading member, and after the loading member contacts the pipette tip, the first elastic member is compressed as the loading member slides; or, the first end is connected to the loading member, the second end is connected to the loading bracket, and after the loading member contacts the pipette tip, the first elastic member is stretched as the loading member slides; The second elastic member has a third end and a fourth end disposed along the first direction, the fourth end being closer than the third end to the end of the loading member used for loading the pipette tip; wherein the third end is connected to the loading bracket, the fourth end is connected to the loading member, and after the first elastic member reaches the first preset deformation amount, the second elastic member is compressed as the loading member slides; or, the third end is connected to the loading member, the fourth end is connected to the loading bracket, and after the first elastic member reaches the first preset deformation amount, the second elastic member is stretched as the loading member slides.

8. The pipetting mechanism according to claim 1, characterized in that, The arrangement direction of the first elastic element and the second elastic element is parallel to the sliding direction of the loading element; Alternatively, the arrangement direction of the first elastic member and the second elastic member intersects the sliding direction of the loading member. The first elastic member has a first end and a second end opposite to each other, and the second elastic member has a third end and a fourth end opposite to each other. The end of the first elastic member facing the loading member for loading the pipette tip is the second end, and the end of the second elastic member facing the loading member for loading the pipette tip is the fourth end. The second end is closer to the loading member end for loading the pipette tip than the fourth end. Alternatively, the second elastic element is sleeved outside the first elastic element, the first elastic element has a first end and a second end opposite to each other, the second elastic element has a third end and a fourth end opposite to each other, the end of the first elastic element facing the end of the loading member for loading the pipette tip is the second end, the end of the second elastic element facing the end of the loading member for loading the pipette tip is the fourth end, and the second end is closer to the end of the loading member for loading the pipette tip than the fourth end.

9. The pipetting mechanism according to claim 1, characterized in that, The pipetting mechanism further includes a third sensing element and a third triggering element. The third sensing element is connected to the loading bracket, and the third triggering element is connected to the loading component and can move synchronously with the loading component. The third sensing element is configured to: be in a signal shielding state during the loading of the pipetting head onto the loading component; be in a signal acquisition state after the pipetting head is loaded onto the loading component; and be triggered by the third triggering element to issue an alarm signal when the first elastic element reaches a fourth preset deformation amount, wherein the fourth preset deformation amount is less than the first preset deformation amount. Alternatively, the third sensing element is configured to: be in a stopped working state during the loading of the pipette head onto the loading component, be in a working state after the pipette head is loaded onto the loading component, and be triggered by the third triggering element to issue an alarm signal when the first elastic element reaches a fourth preset deformation amount, wherein the fourth preset deformation amount is less than the first preset deformation amount.

10. An analyzer, characterized in that, include: The nucleic acid extraction module is used to extract nucleic acids from samples to obtain nucleic acid extract. The pipetting mechanism according to any one of claims 1 to 9 is at least used for transferring the nucleic acid extract into an amplification container; An amplification module is used to amplify the nucleic acid extract loaded in the amplification container to obtain a test solution; The detection module is used to detect the liquid to be tested.

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