Low-temperature storage device for cow egg cells

Abstract

The utility model relates to the technical field of low-temperature storage of cow egg cells, and discloses a low-temperature storage device for cow egg cells, which comprises a fixed frame, supporting legs are fixedly connected to the bottom surface of the fixed frame, a storage mechanism is arranged in the fixed frame, and a dehydration mechanism is arranged on the top surface of the storage mechanism; the storage mechanism comprises a circulating assembly and a placing assembly, the circulating assembly is fixedly connected in the fixing frame, and the placing assembly is fixedly connected to the top surface of the circulating assembly; the placement assembly comprises a placement box, the placement box is arranged on the left side of the liquid nitrogen box, a second water pump is fixedly connected to the surface of the placement box, a first conveying pipe is fixedly connected to the output end of the second water pump, a molecular sieve is fixedly connected into the liquid nitrogen box, and a third water pump is fixedly connected to the surface of the liquid nitrogen box; a one-way air valve is fixedly connected to the top face of the containing box. The low-temperature storage device is provided with the storage mechanism, so that liquid nitrogen is reused, the cost is saved, and damage to egg cells is weakened by arranging the dehydration mechanism.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of low temperature storage for cow ovum, specifically to a low temperature storage device for cow ovum. BACKGROUND

[0002] A low temperature storage device for cow ovum is a specially designed refrigeration equipment for storing cow ovum. The main function of this low temperature storage device is to provide a stable low temperature environment to ensure the activity and quality of cow ovum during storage. Through fine design and advanced technical means, this device can meet the demand of livestock and reproductive medicine field for low temperature storage of cow ovum.

[0003] According to the low temperature storage device for cow ovum (authorization announcement number: CN202499862U) disclosed by the patent network, it is described that "the utility model discloses a low temperature storage device applied to large scale animal cell culture, and is characterized by comprising a storage tank with a closed chamber and a heat preservation jacket sleeved on the storage tank, wherein the storage tank is mainly formed by connecting a top cover and a tank body through a locking mechanism, the top cover is provided with a liquid inlet, a liquid outlet and a gas exchange port for connecting with an air filter, the bottom of the tank body is provided with an openable and closable blowdown port, and one end of the liquid outlet in the storage tank is connected with a liquid guide pipe, the heat preservation jacket comprises an inner shell and an outer shell, and the inner shell and the outer shell have a cavity for containing cooling water, and the outer shell is provided with a cooling water inlet connected with the water outlet end of a cooling circulating water machine and a cooling water outlet connected with the water inlet end of the cooling circulating water machine. The storage device can low temperature store the culture medium needed to be injected into the cell bioreactor or the cell culture liquid discharged from the reactor".

[0004] According to the above description, the applicant believes that the following problems exist:

[0005] In the use process of the utility model, the device does not set up the dehydration mechanism, cannot carry out the dehydration to the ovum cell before the low temperature storage, is easy to cause the damage of the ovum cell, the device does not set up the circulating assembly, makes the device waste a lot of cooling liquid, greatly increases the cost, therefore needs to improve a kind of low temperature storage device for cow ovum to solve the above problems. UTILITY MODEL CONTENTS

[0006] The utility model aims at providing a low temperature storage device for cow ovum to solve the problems raised in the above background.

[0007] In order to achieve the above object, the utility model provides the following technical scheme: A low temperature storage device for cow ovum, including fixed frame, the fixed frame bottom surface is connected with support leg, the fixed frame inside is provided with storage mechanism, the storage mechanism top is provided with dehydration mechanism, the storage mechanism includes circulation component and placement component, the circulation component fixedly connected in the fixed frame inside, the placement component fixedly connected on the circulation component top, the circulation component includes liquid nitrogen tank, the liquid nitrogen tank fixedly connected in the fixed frame inside, the liquid nitrogen tank surface is fixedly connected with transparent window, the liquid nitrogen tank surface is fixedly connected with liquid inlet, the liquid nitrogen tank top is fixedly connected with import, the liquid nitrogen tank inside is fixedly connected with protection shell, the protection shell inside is fixedly connected with storage box, the storage box top is fixedly connected with first water pump, the first water pump output is fixedly connected with egg tube, the storage box top is fixedly connected with air exchange, the liquid nitrogen tank bottom is fixedly connected with outlet, the liquid nitrogen tank bottom is fixedly connected with liquid outlet, the liquid outlet bottom is fixedly connected with limit groove, the limit groove inside is connected with collection box, the liquid outlet surface is fixedly connected with first valve.

[0008] Preferably, the transparent window passes through the liquid nitrogen tank to the inner wall of the storage box, facilitating observation of the ovum condition inside the storage box.

[0009] Preferably, the liquid outlet top surface passes through the liquid nitrogen tank to the inner wall of the storage box, facilitating collection and treatment of the residual liquid inside the storage box.

[0010] Preferably, the placement component includes a placement box, the placement box is arranged on the left side of the liquid nitrogen tank, the placement box surface is fixedly connected with a second water pump, the second water pump output is fixedly connected with a first transport pipe, the liquid nitrogen tank inside is fixedly connected with a molecular sieve, the liquid nitrogen tank surface is fixedly connected with a third water pump, the third water pump output is fixedly connected with a second transport pipe, and the placement box top is fixedly connected with a one-way air valve.

[0011] Preferably, the first transport pipe far away from the second water pump one end is fixedly connected with the liquid outlet, and the second transport pipe far away from the third water pump one end is fixedly connected on the placement box surface, facilitating circulation use of the liquid nitrogen inside the liquid nitrogen tank and saving cost.

[0012] Preferably, the dewatering mechanism includes a dewatering tank arranged at the right side of the fixed frame, a top cover is fixedly connected to the top surface of the dewatering tank, a feeding port is arranged on the top surface of the top cover, a fourth water pump is fixedly connected to the top surface of the top cover, a third conveying pipe is fixedly connected to the output end of the fourth water pump, the distal end of the third conveying pipe away from the fourth water pump is fixedly connected to the inside of the liquid inlet, a motor is fixedly connected to the inside of the dewatering tank, a stirring rod is fixedly connected to the output end of the motor, stirring blades are fixedly connected to the surface of the stirring rod, a screen is fixedly connected to the bottom surface of the dewatering tank, a drain pipe is fixedly connected to the bottom surface of the dewatering tank, and a second valve is fixedly connected to the surface of the drain pipe, so that the egg cells are first subjected to dewatering treatment, thereby reducing the damage of the egg cells in the freezing process.

[0013] Compared with the prior art, the low-temperature storage device for cow egg cells has the following beneficial effects:

[0014] 1. The low-temperature storage device for cow egg cells is provided with a storage mechanism, a placing assembly is arranged, a second water pump is arranged, a first conveying pipe is arranged, liquid nitrogen in the liquid nitrogen tank after use is collected and treated, a third water pump and a second conveying pipe are arranged, the used liquid nitrogen can be placed in the placing box, a one-way air valve is arranged, the gas mixed in the liquid nitrogen can be removed, the liquid nitrogen can be reused, and cost is saved; a circulating assembly is arranged, the egg cells can be stored, and a transparent window is arranged, so that the condition of the egg cells in the storage box can be better observed.

[0015] 2. The low-temperature storage device for cow egg cells is provided with a dewatering mechanism, a dewatering solution is placed in the dewatering tank through the feeding port, a motor and stirring blades are arranged, the egg cells can be fully dewatered, and the damage of the egg cells in the freezing process can be greatly reduced. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor under the premise of the drawings:

[0017] Figure 1 It is an appearance structure schematic view of the present application;

[0018] Figure 2 It is an appearance structure schematic view of the present application;

[0019] Figure 3 It is a front sectional view structure schematic view of the present application;

[0020] Figure 4 It is a front cross-section structure schematic view of the circulating assembly of the utility model.

[0021] Figure 5 It is a front cross-section structure schematic view of the dehydration assembly of the utility model.

[0022] In the figure: 1, storage mechanism; 11, circulating assembly; 111, liquid nitrogen tank; 112, transparent window; 113, protective shell; 114, storage tank; 115, first water pump; 116, egg outlet pipe; 117, air inlet; 118, inlet; 119, liquid inlet; 1110, sewage outlet; 1111, liquid outlet; 1112, limiting groove; 1113, collection tank; 1114, first valve; 12, placing assembly; 121, placing tank; 122, molecular sieve; 123, one-way air valve; 124, first conveying pipe; 125, second water pump; 126, second conveying pipe; 127, third water pump; 2, fixed frame; 3, supporting leg; 4, dehydration mechanism; 41, dehydration tank; 42, top cover; 43, feed inlet; 44, fourth water pump; 45, third conveying pipe; 46, motor; 47, stirring rod; 48, stirring blade; 49, screen; 410, drain pipe; 411, second valve. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] In the utility model, unless explicitly defined and limited, the terms "mounting", "connection", "connecting", "fixing" and the like should be understood broadly, for example, can be fixed connection, or can be detachable connection, or can be integrated; can be mechanical connection, or can be electrical connection; can be directly connected, or can be indirectly connected through an intermediate medium; can be the communication inside two elements or the interaction relationship between two elements. For those skilled in the art, the specific meanings of the above terms in the utility model can be understood according to specific circumstances.

[0025] Embodiment one:

[0026] Based on the fact that the prior art low-temperature storage device for cow ovum cells damages the ovum cells too much, it is inconvenient to observe the internal ovum cells during storage, and a large amount of liquid nitrogen and other resources are consumed, the embodiment provides a low-temperature storage device for cow ovum cells, please refer to Figures 1-3The utility model provides a kind of low-temperature storage device for cow ovum, can save cost, greatly reduce the damage to ovum, the low-temperature storage device for cow ovum includes fixed frame 2, and fixed frame 2 bottom surface is fixedly connected with support leg 3, and fixed frame 2 inside is provided with storage mechanism 1, and the dehydration mechanism 4 is arranged on the top surface of storage mechanism 1;Storage mechanism 1 includes circulation assembly 11 and placing assembly 12, and circulation assembly 11 is fixedly connected in fixed frame 2 inside, and placing assembly 12 is fixedly connected on the top surface of circulation assembly 11;Circulation assembly 11 includes liquid nitrogen tank 111, and liquid nitrogen tank 111 is fixedly connected in fixed frame 2 inside, and transparent window 112 is fixedly connected on the surface of liquid nitrogen tank 111, and inlet 119 is fixedly connected on the surface of liquid nitrogen tank 111, and inlet 118 is fixedly connected on the top surface of liquid nitrogen tank 111, and protection shell 113 is fixedly connected in liquid nitrogen tank 111 inside, and storage tank 114 is fixedly connected in protection shell 113 inside, and first water pump 115 is fixedly connected on the top surface of storage tank 114, and outlet egg tube 116 is fixedly connected on the output end of first water pump 115, and air exchange port 117 is fixedly connected on the top surface of storage tank 114, and outlet 1110 is fixedly connected on the bottom surface of liquid nitrogen tank 111, and outlet 1111 is fixedly connected on the bottom surface of liquid nitrogen tank 111, and limit groove 1112 is fixedly connected on the bottom surface of outlet 1111, and collection tank 1113 is slidably connected in limit groove 1112 inside, and valve is fixedly connected on the surface of outlet 1111.

[0027] Transparent window 112 passes through liquid nitrogen tank 111 to the inner wall of storage tank 114, to facilitate the observation of the ovum condition in the inside of storage tank 114.

[0028] Outlet 1111 top surface passes through liquid nitrogen tank 111 to the inner wall of storage tank 114, to facilitate the collection and processing of residual liquid in the inside of storage tank 114.

[0029] Example two:

[0030] On the basis of example one, refer to Figures 4-5 The device can not make liquid nitrogen be reused when carrying out low-temperature storage to ovum, increase cost, therefore the device is also provided with placing assembly 12, and placing assembly 12 includes placing tank 121, and placing tank 121 is arranged on the left side of liquid nitrogen tank 111, and second water pump 125 is fixedly connected on the surface of placing tank 121, and first transport pipe 124 is fixedly connected on the output end of second water pump 125, and molecular sieve 122 is fixedly connected in liquid nitrogen tank 111, and third water pump 127 is fixedly connected on the surface of liquid nitrogen tank 111, and second transport pipe 126 is fixedly connected on the output end of third water pump 127, and one-way air valve 123 is fixedly connected on the top surface of placing tank 121, and liquid nitrogen is handled after use, so that liquid nitrogen can be reused, save cost.

[0031] The first conveying pipe 124 is fixedly connected with the liquid outlet 1111 away from one end of the second water pump 125, and the second conveying pipe 126 is fixedly connected on the surface of the placing box 121 away from one end of the third water pump 127, so that the liquid nitrogen in the liquid nitrogen tank 111 can be recycled, and cost is saved.

[0032] In order to reduce the damage to the egg cells, the device is also provided with a dehydration mechanism 4, which comprises a dehydration tank 41 arranged on the right side of the fixed frame 2, a top cover 42 fixedly connected to the top surface of the dehydration tank 41, an inlet 43 formed in the top surface of the top cover 42, a fourth water pump 44 fixedly connected to the top surface of the top cover 42, a third conveying pipe 45 fixedly connected to the inlet 119 away from one end of the fourth water pump 44, a motor 46 fixedly connected in the dehydration tank 41, a stirring rod 47 fixedly connected to the output end of the motor 46, stirring blades 48 fixedly connected to the surface of the stirring rod 47, a screen 49 fixedly connected to the bottom surface of the dehydration tank 41, a drain pipe 410 fixedly connected to the bottom surface of the dehydration tank 41, and a second valve 411 fixedly connected to the surface of the drain pipe 410, so that the egg cells are first subjected to dehydration treatment, thereby reducing the damage to the egg cells during freezing.

[0033] In actual operation, when the device is used, first, the dehydration solution is placed in the dehydration tank 41 through the inlet 43, and then the egg cells are placed in, the motor 46 is turned on, the stirring rod 47 is rotated by the motor 46, the stirring blades 48 are rotated by the stirring rod 47, after the egg cells are in contact with the dehydration solution, the second valve 411 is opened, the dehydration solution is discharged through the screen 49, then the second valve 411 is closed, the fourth water pump 44 is opened, and the third conveying pipe 45 is used to transport the dehydrated egg cells to the storage tank 114, the liquid is placed in the liquid nitrogen tank 111 through the inlet 118, so that the egg cells are stored at low temperature, when used, the liquid nitrogen in the liquid nitrogen tank 111 is discharged to the first conveying pipe 124 through the liquid outlet 1111, the used liquid nitrogen is transported to the placing box 121 through the opening of the second water pump 125, the liquid nitrogen is treated by standing through the molecular sieve 122 and the one-way air valve 123, the treated liquid nitrogen is transported back to the liquid nitrogen tank 111 through the opening of the third water pump 127 and the second conveying pipe 126, cost is saved, the egg cells in the storage tank 114 are discharged through the first water pump 115 and the egg outlet pipe 116, after the work is completed, the first valve 1114 is opened, and the residual liquid in the storage tank is discharged to the collection tank 1113, which is convenient for treatment.

[0034] It is to be noted that, as used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to "a component" can include a combination of two or more components. Additionally, the terms "comprise," "comprises," and "comprising," or any variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements is not necessarily limited to those elements, but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. Furthermore, unless otherwise indicated herein, the terms "first," "second," "third," etc., are not intended to denote a physical order, quantity, or importance, but rather are used for the purpose of nomenclature.

Claims

1. A device for cryopreservation of cow oocytes comprising a fixed frame (2) characterised in that: The bottom surface of the fixed frame (2) is fixedly connected with a supporting leg (3), and the inside of the fixed frame (2) is provided with a storage mechanism (1), and the top surface of the storage mechanism (1) is provided with a dehydration mechanism (4); The storage mechanism (1) comprises a circulating assembly (11) and a placing assembly (12), the circulating assembly (11) is fixedly connected in the fixed frame (2), and the placing assembly (12) is fixedly connected to the top surface of the circulating assembly (11); The circulating assembly (11) comprises a liquid nitrogen tank (111), the liquid nitrogen tank (111) is fixedly connected in the fixed frame (2), a transparent window (112) is fixedly connected to the surface of the liquid nitrogen tank (111), a liquid inlet (119) is fixedly connected to the surface of the liquid nitrogen tank (111), an inlet (118) is fixedly connected to the top surface of the liquid nitrogen tank (111), a protection shell (113) is fixedly connected in the liquid nitrogen tank (111), a storage tank (114) is fixedly connected in the protection shell (113), a first water pump (115) is fixedly connected to the top surface of the storage tank (114), an egg outlet tube (116) is fixedly connected to the output end of the first water pump (115), an air exchange port (117) is fixedly connected to the top surface of the storage tank (114), a sewage outlet (1110) is fixedly connected to the bottom surface of the liquid nitrogen tank (111), a liquid outlet (1111) is fixedly connected to the bottom surface of the liquid nitrogen tank (111), a limiting groove (1112) is fixedly connected to the bottom surface of the liquid outlet (1111), a collection tank (1113) is slidably connected in the limiting groove (1112), and a first valve (1114) is fixedly connected to the surface of the liquid outlet (1111).

2. A device for cryopreservation of oocytes in cows as claimed in claim 1, wherein: The transparent window (112) penetrates through the liquid nitrogen tank (111) to the inner wall of the storage tank (114).

3. A device for cryopreservation of oocytes in cows as claimed in claim 1 wherein: The top surface of the liquid outlet (1111) penetrates through the liquid nitrogen tank (111) to the inner wall of the storage tank (114).

4. A device for cryopreservation of oocytes in cows as claimed in claim 1, wherein: The placing assembly (12) comprises a placing tank (121), the placing tank (121) is arranged on the left side of the liquid nitrogen tank (111), a second water pump (125) is fixedly connected to the surface of the placing tank (121), a first conveying pipe (124) is fixedly connected to the output end of the second water pump (125), a molecular sieve (122) is fixedly connected in the liquid nitrogen tank (111), a third water pump (127) is fixedly connected to the surface of the liquid nitrogen tank (111), a second conveying pipe (126) is fixedly connected to the output end of the third water pump (127), and a one-way air valve (123) is fixedly connected to the top surface of the placing tank (121).

5. A device for cryopreservation of oocytes in a cow according to claim 4, characterized in that: The first conveying pipe (124) is fixedly connected to the liquid outlet (1111) away from the second water pump (125), and the second conveying pipe (126) is fixedly connected to the surface of the placing tank (121) away from the third water pump (127).

6. A device for cryopreservation of oocytes in cows as claimed in claim 1, wherein: The dewatering mechanism (4) includes a dewatering box (41), which is arranged at the right side of the fixed frame (2). The top surface of the dewatering box (41) is fixedly connected with a top cover (42). The top surface of the top cover (42) is provided with an inlet (43). The top surface of the top cover (42) is fixedly connected with a fourth water pump (44). The output end of the fourth water pump (44) is fixedly connected with a third conveying pipe (45). The far end of the third conveying pipe (45) from the fourth water pump (44) is fixedly connected inside the liquid inlet (119). The inside of the dewatering box (41) is fixedly connected with a motor (46). The output end of the motor (46) is fixedly connected with a stirring rod (47). The surface of the stirring rod (47) is fixedly connected with stirring blades (48). The bottom surface of the dewatering box (41) is fixedly connected with a screen (49). The bottom surface of the dewatering box (41) is fixedly connected with a drain pipe (410). The surface of the drain pipe (410) is fixedly connected with a second valve (411).

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