Reactant mixing device for biological detection

Abstract

The utility model provides a reactant mixing device for biological detection, which belongs to the technical field of cell detection and comprises a mixing box, and a filtering mechanism, an isolating mechanism and a waste liquid guide pipe are arranged in the mixing box. The interior of the mixing box is divided into a reaction area X, a transition area Y and a mixing post-treatment area Z from left to right through the filtering mechanism and the isolation mechanism, the waste liquid flow guide pipe penetrates through the bottom of the mixing box, one end of the waste liquid flow guide pipe is arranged in the mixing post-treatment area Z, a siphon mechanism is arranged in the mixing post-treatment area Z, and the other end of the waste liquid flow guide pipe is arranged in the mixing post-treatment area Z. The siphon mechanism is arranged on the waste liquid flow guide pipe in a sleeving manner; the siphon mechanism comprises an upper cover and a connecting pipe, and can solve the problem that in biological detection, some impurities or incompletely reacted substances may be generated after reactants are mixed, and if effective filtration and separation are not performed, the impurities interfere with subsequent detection and analysis, resulting in inaccurate detection results.

Description

TECHNICAL FIELD

[0001] The utility model belongs to cell detection technical field, and specifically, it relates to a kind of reaction mixture mixing devices for biological detection. BACKGROUND

[0002] Biological detection refers to the detection of substances within an organism (such as blood, urine, tissue samples) or environmental samples outside the organism (such as water, soil microorganisms, etc.), to obtain information about the health of individual organisms, ecological environment or other related information. Common methods of biological detection include biochemical detection methods: based on biochemical reactions to detect target substances. For example, using enzyme-catalyzed reactions to detect glucose content in blood. Colorimetric method is also a common biochemical detection method. It is based on the color change of substances under certain conditions and certain reagents, and the color depth is determined by colorimeter or spectrophotometer, so as to determine the content of the substance. Immunological detection method: using the specific binding of antigens and antibodies to detect target substances. Enzyme-linked immunosorbent assay (ELISA) is a widely used immunological detection method. Molecular biology detection method: polymerase chain reaction (PCR) is a powerful molecular biology detection technology. It can rapidly amplify specific DNA fragments in vitro.

[0003] In biological detection, after mixing the reactants, some impurities or incompletely reacted substances may be produced, and if effective filtration and separation are not performed, these impurities will interfere with subsequent detection analysis, resulting in inaccurate detection results. UTILITY MODEL CONTENT

[0004] Therefore, the utility model provides a kind of reaction mixture mixing devices for biological detection, to solve in biological detection, after mixing the reactants, some impurities or incompletely reacted substances may be produced, and if effective filtration and separation are not performed, these impurities will interfere with subsequent detection analysis, resulting in inaccurate detection results.

[0005] The utility model is realized as follows:

[0006] The utility model provides a kind of reaction mixture mixing devices for biological detection, wherein: including mixing box, the filter mechanism, isolation mechanism and waste liquid flow guide pipe are equipped in the mixing box inside, the filter mechanism and the isolation mechanism divide the mixing box from left to right into reaction zone X, transition zone Y and mixed post-processing zone Z, the waste liquid flow guide pipe penetrates the mixing box bottom, and one end of the waste liquid flow guide pipe is set in the mixed post-processing zone Z, the mixed post-processing zone Z is equipped with siphon mechanism, the siphon mechanism is sleeved on the waste liquid flow guide pipe.

[0007] On the basis of the above technical solution, the reaction mixture mixing device for biological detection of the utility model can also be improved as follows:

[0008] The siphon mechanism comprises an upper cover and a connecting pipe, the upper cover is arranged on the top of the connecting pipe, the connecting pipe is sleeved on the outer periphery of the waste liquid guide pipe, and the bottom of the connecting pipe is arranged in a spaced manner with the bottom of the mixing box, and the upper cover is arranged in a spaced manner with the top of the waste liquid guide pipe.

[0009] Further, the filtering mechanism comprises a filter sheet and a biological filter layer, the filter sheet is longitudinally arranged in the mixing box and divides the mixing box, the filter sheet is provided with a through hole, and the biological filter layer is arranged in the through hole.

[0010] Further, the through holes are arranged in a matrix manner in the upper part of the filter sheet.

[0011] Further, the isolation mechanism comprises an isolation plate and a guide plate, the isolation plate is longitudinally arranged at the bottom of the mixing box, the guide plate is longitudinally arranged at the top of the mixing box, and the guide plate and the isolation plate form a guide opening.

[0012] Further, the guide plate is a folding plate, the lower end of the folding plate extends into the mixing post-treatment area Z, and the height of the lower end of the folding plate is lower than the height of the top of the isolation plate, and a guide channel is arranged between the isolation plate and the guide plate.

[0013] Further, the top of the mixing box is provided with a sealable sealing cover, and the sealing cover is provided with a rubber sealing gasket at the edge in contact with the mixing box.

[0014] Further, the bottom of the mixing box is provided with a heating mechanism, and the heating mechanism is attached to the bottom of the mixing box.

[0015] Further, the inner wall of the mixing box is coated with an anti-corrosion coating.

[0016] Further, the anti-corrosion coating is made of polytetrafluoroethylene material.

[0017] Compared with the prior art, the reaction mixture mixing device for biological detection has the following advantages:

[0018] By arranging the filtering mechanism, the filter sheet with the biological filter layer, and the through holes arranged in a matrix manner in the upper part, impurities and unreacted substances generated in the reaction mixture mixing process can be effectively intercepted, the purity of the reaction mixture entering the subsequent area is ensured, and the accuracy and reliability of the detection result are improved.

[0019] The isolation plate and the flow guide plate in the isolation mechanism cooperate to form reasonable flow guide openings and flow guide channels, ensure that the reactants in the reaction zone X can be fully mixed and orderly pass through the transition zone Y into the mixed post-processing zone Z, improve the reaction efficiency, reduce the uncertainty in the reaction process, and make the experimental results more repeatable and stable.

[0020] The siphon mechanism can realize stable discharge of waste liquid in the mixed post-processing zone Z, prevent waste liquid accumulation; the sealing cover ensures that the internal environment is not polluted by the outside world, the heating mechanism can meet the temperature requirements of specific biological reactions, and the anti-corrosion coating prolongs the service life of the device, which collectively provides a stable and suitable environment for biological detection reactions, is conducive to improving the detection accuracy and efficiency, and reduces the maintenance cost and experimental error of the device.

[0021] The siphon mechanism can realize stable discharge of waste liquid in the mixed post-processing zone Z, prevent waste liquid accumulation; the sealing cover ensures that the internal environment is not polluted by the outside world, the heating mechanism can meet the temperature requirements of specific biological reactions, and the anti-corrosion coating prolongs the service life of the device, which collectively provides a stable and suitable environment for biological detection reactions, is conducive to improving the detection accuracy and efficiency, and reduces the maintenance cost and experimental error of the device. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the description of the embodiments of the present application. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0023] Fig. 1 It is a structural schematic view of a reactant mixing device for biological detection.

[0024] Fig. 2 It is a sectional view of a reactant mixing device for biological detection.

[0025] Fig. 3 It is a structural schematic view of a filtering mechanism.

[0026] In the drawings, the components represented by each reference numeral are listed as follows:

[0027] 1, mixing box; 2, waste liquid flow guide pipe; 3, siphon mechanism; 31, upper cover; 32, connecting pipe; 4, filtering mechanism; 41, filter piece; 42, biological filter layer; 5, isolation mechanism; 51, isolation plate; 52, flow guide plate; 6, sealing cover; 7, heating mechanism. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be clearly and completely described below in combination with the drawings in the embodiments of the present application.

[0029] As Figs. 1-3 shown is a first embodiment of a reaction mixture mixing device for biological detection provided by the utility model, in the embodiment: including mixing box 1, the inside of mixing box 1 is equipped with filter mechanism 4, isolation mechanism 5 and waste liquid flow guide pipe 2, filter mechanism 4 and isolation mechanism 5 divide the inside of mixing box 1 from left to right into reaction zone X, transition zone Y and mixed post-processing zone Z, waste liquid flow guide pipe 2 penetrates the bottom of mixing box 1, and one end of waste liquid flow guide pipe 2 is arranged in mixed post-processing zone Z, siphon mechanism 3 is arranged in mixed post-processing zone Z, and siphon mechanism 3 is sleeved on waste liquid flow guide pipe 2.

[0030] When using, check whether the sealing cover 6 of the mixing box 1 is closed tightly, ensure that the rubber sealing washer plays a good sealing role, prevent foreign matters from entering the mixing box 1 and affecting the reaction. According to the demand of the biological reaction to be detected, set the appropriate reaction temperature through the heating mechanism 7, and open the heating mechanism 7 in advance to make the temperature in the mixing box 1 reach the preset value, ensure the stability of the reaction environment. Open the sealing cover 6, and then add the reactants required for biological detection into the reaction zone X in sequence, and then close the sealing cover 6. Start the mixing device (if there is a stirring and mixing function), so that the reactants are fully mixed in the reaction zone X, and in the mixing process, the filter mechanism 4 starts to work and preliminarily filters the reactants to remove possible impurities. With the progress of the reaction, the reactants flow into the mixed post-processing zone Z from the reaction zone X through the transition zone Y under the action of the isolation mechanism 5 through the flow guide port and the flow guide channel. In this process, the folded plate structure of the flow guide plate 52 helps to guide the flow of liquid, ensures that the reaction products can smoothly enter the mixed post-processing zone Z, and the isolation plate 51 prevents the substances that have not been fully reacted from entering the subsequent area too early. When the waste liquid in the mixed post-processing zone Z reaches a certain amount, the siphon mechanism 3 is automatically started (which can be controlled by the liquid level difference or other control methods), and the waste liquid is discharged outside the device through the connecting pipe 32 and the waste liquid flow guide pipe 2. At this time, the flow rate of the waste liquid can be controlled through the flow regulating valve on the waste liquid flow guide pipe 2, so as to ensure the stability of the siphon process and not to affect the reaction environment in the device.

[0031] In the above technical solution, the siphon mechanism 3 includes an upper cover 31 and a connecting pipe 32, the upper cover 31 is arranged on the top of the connecting pipe 32, the connecting pipe 32 is sleeved on the outer periphery of the waste liquid flow guide pipe 2, and the bottom of the connecting pipe 32 is arranged in a spaced manner with the bottom of the mixing box 1, and the upper cover 31 is arranged in a spaced manner with the top of the waste liquid flow guide pipe 2.

[0032] Further, in the above technical solution, the filter mechanism 4 includes a filter sheet 41 and a biological filter layer 42, the filter sheet 41 is longitudinally arranged in the mixing box 1 and divides the mixing box 1, the filter sheet 41 is provided with a through hole, and the biological filter layer 42 is arranged in the through hole.

[0033] Further, in the above technical solution, the number of through holes is several, and the several through holes are located in the upper part of the filter sheet 41, and the through holes are arranged in a matrix.

[0034] Further, in the above technical solution, the isolation mechanism 5 includes an isolation plate 51 and a flow guide plate 52, the isolation plate 51 is longitudinally arranged on the bottom of the mixing box 1, the flow guide plate 52 is longitudinally arranged on the top of the mixing box 1, and the flow guide opening is formed between the isolation plate 51 and the flow guide plate 52.

[0035] Further, in the above technical solution, the flow guide plate 52 is a folded plate, the lower end of the folded plate extends into the mixing post-processing area Z, and the height of the lower end of the folded plate is lower than the height of the top of the isolation plate 51, and the flow guide channel is arranged between the isolation plate 51 and the flow guide plate 52.

[0036] Further, in the above technical solution, the top of the mixing box 1 is provided with a sealable sealing cover 6, and the sealing cover 6 is provided with a rubber sealing ring at the edge in contact with the mixing box 1.

[0037] Further, in the above technical solution, the bottom of the mixing box 1 is provided with a heating mechanism 7, and the heating mechanism 7 is attached to the bottom of the mixing box 1.

[0038] Further, in the above technical solution, the inner wall of the mixing box 1 is coated with an anti-corrosion coating.

[0039] Further, in the above technical solution, the anti-corrosion coating is made of polytetrafluoroethylene material.

[0040] Specific, the principle of the utility model is: check whether the sealing cover 6 of mixed box 1 is closed tightly, ensure that rubber sealing washer plays good sealing effect, prevent foreign matter from entering mixed box 1 and influencing reaction. According to the demand of the biological reaction to be detected, through heating mechanism 7 sets suitable reaction temperature, and opens heating mechanism 7 in advance to make the temperature in mixed box 1 reach preset value, ensures that the reaction environment is stable. Open the sealing cover 6, and then close the sealing cover 6 after adding the reactant required by biological detection into reaction area X in turn. Start mixing device (if there is mixing function such as stirring), and make the reactant mix fully in reaction area X, and filtering mechanism 4 starts working in the mixing process, and carries out preliminary filtration to the reactant, and removes possible impurities. Along with the reaction, the reactant flows gradually into mixed post-processing area Z from reaction area X through transition area Y under the action of isolation mechanism 5 through flow guide port and flow guide channel. In this process, the folding plate structure of flow guide plate 52 helps to guide liquid flow, ensures that reaction product can enter mixed post-processing area Z smoothly, and isolation plate 51 prevents the material not fully reacted from entering subsequent area prematurely. When the waste liquid in mixed post-processing area Z reaches a certain amount, siphon mechanism 3 is started automatically (can be controlled by liquid level difference or other control mode), and the waste liquid is discharged outside the device through connecting pipe 32 and waste liquid flow guide pipe 2. At this time, the flow rate of waste liquid discharge can be controlled through flow regulating valve on waste liquid flow guide pipe 2, ensures that the siphon process is stable, and does not affect the reaction environment in the device.

Claims

1. A reagent mixing device for biological detection, characterized by: The utility model provides a mixed box (1) is equipped with filter mechanism (4), isolation mechanism (5) and waste liquid guide pipe (2) inside, filter mechanism (4) and isolation mechanism (5) divide the mixed box (1) from left to right into reaction area X, transition area Y and mixed post -treatment area Z in, waste liquid guide pipe (2) penetrates the bottom of mixed box (1), and one end of waste liquid guide pipe (2) is located in mixed post -treatment area Z, and mixed post -treatment area Z is equipped with siphon mechanism (3), and siphon mechanism (3) is sleeved on waste liquid guide pipe (2).

2. The reagent mixing device for biological detection according to claim 1, wherein The siphon mechanism (3) includes an upper cover (31) and a connecting pipe (32), the upper cover (31) is arranged on the top of the connecting pipe (32), the connecting pipe (32) is sleeved on the outer periphery of the waste liquid guide pipe (2), and the bottom of the connecting pipe (32) is spaced apart from the bottom of the mixed box (1), and the upper cover (31) is spaced apart from the top of the waste liquid guide pipe (2).

3. The reagent mixing device for biological detection according to claim 2, wherein The filter mechanism (4) includes a filter sheet (41) and a biological filter layer (42), the filter sheet (41) is longitudinally arranged in the mixed box (1) and divides the mixed box (1), the filter sheet (41) is provided with a through hole, and the biological filter layer (42) is arranged in the through hole.

4. The reagent mixing device for biological detection according to claim 3, wherein The number of through holes is several, and the through holes are arranged in the upper part of the filter sheet (41) in a matrix manner.

5. The reagent mixing device for biological detection according to claim 4, wherein The isolation mechanism (5) includes an isolation plate (51) and a guide plate (52), the isolation plate (51) is longitudinally arranged at the bottom of the mixed box (1), and the guide plate (52) is longitudinally arranged at the top of the mixed box (1), and the isolation plate (51) and the guide plate (52) form a guide opening.

6. The reagent mixing device for biological detection according to claim 5, wherein The guide plate (52) is a folding plate, the lower end of the folding plate extends into the mixed post-treatment area Z, and the height of the lower end of the folding plate is lower than the height of the top of the isolation plate (51), and a guide channel is arranged between the isolation plate (51) and the guide plate (52).

7. The reagent mixing device for biological detection according to claim 6, wherein The top of the mixed box (1) is provided with a sealable sealing cover (6), and the sealing cover (6) is provided with a rubber sealing ring at the edge in contact with the mixed box (1).

8. The reagent mixing device for biological detection according to claim 7, wherein The bottom of the mixed box (1) is provided with a heating mechanism (7), and the heating mechanism (7) is attached to the bottom of the mixed box (1).

9. The reagent mixing device for biological detection according to claim 8, wherein The inner wall of the mixed box (1) is coated with an anticorrosive coating.

10. The reagent mixing device for biological detection according to claim 9, wherein The anticorrosive coating is made of polytetrafluoroethylene material.

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